| characterization of bacteria in ballast water using maldi-tof mass spectrometry. | to evaluate a rapid and cost-effective method for monitoring bacteria in ballast water, several marine bacterial isolates were characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms). since international maritime organization (imo) regulations are concerned with the unintended transportation of pathogenic bacteria through ballast water, emphasis was placed on detecting species of vibrio, enterococci and coliforms. seawater samples collected fr ... | 2012 | 22685576 |
| systems biology of microbial exopolysaccharides production. | exopolysaccharides (epss) produced by diverse group of microbial systems are rapidly emerging as new and industrially important biomaterials. due to their unique and complex chemical structures and many interesting physicochemical and rheological properties with novel functionality, the microbial epss find wide range of commercial applications in various fields of the economy such as food, feed, packaging, chemical, textile, cosmetics and pharmaceutical industry, agriculture, and medicine. epss ... | 2015 | 26734603 |
| comparison of traditional phenotypic identification methods with partial 5' 16s rrna gene sequencing for species-level identification of nonfermenting gram-negative bacilli. | correct identification of nonfermenting gram-negative bacilli (nfb) is crucial for patient management. we compared phenotypic identifications of 96 clinical nfb isolates with identifications obtained by 5' 16s rrna gene sequencing. sequencing identified 88 isolates (91.7%) with >99% similarity to a sequence from the assigned species; 61.5% of sequencing results were concordant with phenotypic results, indicating the usability of sequencing to identify nfb. | 2010 | 20164273 |
| pseudomonas oleovorans subsp. lubricantis subsp. nov., and reclassification of pseudomonas pseudoalcaligenes atcc 17440t as later synonym of pseudomonas oleovorans atcc 8062 t. | isolate rs1(t) isolated from used metalworking fluid was found to be a gram-negative, motile, and non-spore forming rod. based on phylogenetic analyses with 16s rrna, isolate rs1(t) was placed into the mendocina sublineage of pseudomonas. the major whole cell fatty acids were c(18:1)omega7c (32.6%), c(16:0) (25.5%), and c(15:0) iso 2oh/c(16:1)omega7c (14.4%). the sequence similarities of isolate rs1(t) based on gyrb and rpod genes were 98.9 and 98.0% with pseudomonas pseudoalcaligenes, and 98.5 ... | 2010 | 19936829 |
| changes in microbial community composition and function during a polyaromatic hydrocarbon phytoremediation field trial. | the purpose of this study was to investigate the mechanism by which phytoremediation systems promote hydrocarbon degradation in soil. the composition and degradation capacity of the bulk soil microbial community during the phytoremediation of soil contaminated with aged hydrocarbons was assessed. in the bulk soil, the level of catabolic genes involved in hydrocarbon degradation (ndob, alkb, and xyle) as well as the mineralization of hexadecane and phenanthrene was higher in planted treatment cel ... | 2003 | 12514031 |
| polyhydroxyalkanoate granules are complex subcellular organelles (carbonosomes). | | 2009 | 19270094 |
| substrate interactions during the biodegradation of btex and thf mixtures by pseudomonas oleovorans dt4. | the efficient tetrahydrofuran (thf)-degrading bacterium, pseudomonas oleovorans dt4 was used to investigate the substrate interactions during the aerobic biotransformation of thf and btex mixtures. benzene and toluene could be utilized as growth substrates by dt4, whereas cometabolism of m-xylene, p-xylene and ethylbenzene occurred with thf. in binary mixtures, thf degradation was delayed by xylene, ethylbenzene, toluene and benzene in descending order of inhibitory effects. conversely, benzene ... | 2011 | 21511464 |
| biotechnological strategies to improve production of microbial poly-(3-hydroxybutyrate): a review of recent research work. | poly-(3-hydroxybutyrate) [p(3hb)] is a polyester synthesized as a carbon and energy reserve material by a wide number of bacteria. this polymer is characterized by its thermo-plastic properties similar to plastics derived from petrochemical industry, such as polyethylene and polypropylene. furthermore, p(3hb) is an inert, biocompatible and biodegradable material which has been proposed for several uses in medical and biomedical areas. currently, only few bacterial species such as cupriavidus nec ... | 2014 | 24898500 |
| modeling lipid accumulation in oleaginous fungi in chemostat cultures. ii: validation of the chemostat model using yeast culture data from literature. | a model that predicts cell growth, lipid accumulation and substrate consumption of oleaginous fungi in chemostat cultures (meeuwse et al. in bioproc biosyst eng. doi: 10.1007/s00449-011-0545-8 , 2011) was validated using 12 published data sets for chemostat cultures of oleaginous yeasts and one published data set for a poly-hydroxyalkanoate accumulating bacterial species. the model could describe all data sets well with only minor modifications that do not affect the key assumptions, i.e. (1) ol ... | 2011 | 21516366 |
| rapid detection of rrna group i pseudomonads in contaminated metalworking fluids and biofilm formation by fluorescent in situ hybridization. | metalworking fluids (mwfs), used in different machining operations, are highly prone to microbial degradation. microbial communities present in mwfs lead to biofilm formation in the mwf systems, which act as a continuous source of contamination. species of rrna group i pseudomonas dominate in contaminated mwfs. however, their actual distribution is typically underestimated when using standard culturing techniques as most fail to grow on the commonly used pseudomonas isolation agar. to overcome t ... | 2011 | 22042232 |
| recent advances in petroleum microbiology. | recent advances in molecular biology have extended our understanding of the metabolic processes related to microbial transformation of petroleum hydrocarbons. the physiological responses of microorganisms to the presence of hydrocarbons, including cell surface alterations and adaptive mechanisms for uptake and efflux of these substrates, have been characterized. new molecular techniques have enhanced our ability to investigate the dynamics of microbial communities in petroleum-impacted ecosystem ... | 2003 | 14665675 |
| identification of the haloarchaeal type phasin (phap) that functions in polyhydroxyalkanoate accumulation and granule formation in haloferax mediterranei. | the polyhydroxyalkanoate (pha) granule-associated proteins (pgaps) are important for pha synthesis and granule formation, but currently little is known about the haloarchaeal pgaps. this study focused on identification and functional analysis of the pgaps in a haloarchaeon haloferax mediterranei. these pgaps were visualized with two-dimensional gel electrophoresis (2-de) and identified by maldi tof/tof ms. the most abundant protein on the granules was identified as a hypothetical protein, design ... | 2012 | 22247127 |
| diversity of endophytic bacteria in malaysian plants as revealed by 16s rrna encoding gene sequence based method of bacterial identification. | bacterial endophytes do have several potential applications in pharmacy, medicine and agricultural biotech industry. the main objective of this study was to understand types of bacterial endophytes associated with dicotyledonous (dicot) and monocotyledonous (monocot) plant species. isolation of the endophytic bacteria was performed using surface-sterilized various tissue samples, and identification of the endophytic bacterial isolates (ebis) was completed using 16s rrna encoding gene sequence si ... | 2013 | 24396249 |
| impurities of crude glycerol and their effect on metabolite production. | glycerol is a valuable raw material for the production of industrially useful metabolites. among many promising applications for the use of glycerol is its bioconversion to high value-added compounds, such as 1,3-propanediol (1,3-pd), succinate, ethanol, propionate, and hydrogen, through microbial fermentation. another method of waste material utilization is the application of crude glycerol in blends with other wastes (e.g., tomato waste hydrolysate). however, crude glycerol, a by-product of bi ... | 2014 | 25100926 |
| carbon sources for polyhydroxyalkanoates and an integrated biorefinery. | polyhydroxyalkanoates (phas) are a group of bioplastics that have a wide range of applications. extensive progress has been made in our understanding of phas' biosynthesis, and currently, it is possible to engineer bacterial strains to produce phas with desired properties. the substrates for the fermentative production of phas are primarily derived from food-based carbon sources, raising concerns over the sustainability of their production in terms of their impact on food prices. this paper give ... | 2016 | 27447619 |
| draft genome sequence of pseudomonas sp. eps/l25, isolated from the medicinal plant echinacea purpurea and able to synthesize antimicrobial compounds. | we announce here the draft genome sequence of pseudomonas sp. strain eps/l25, isolated from the stem/leaves of the medicinal plant echinacea purpurea this genome will allow for comparative genomics in order to identify genes associated with the production of bioactive compounds and antibiotic resistance. | 2016 | 27151804 |
| impurities of crude glycerol and their effect on metabolite production. | glycerol is a valuable raw material for the production of industrially useful metabolites. among many promising applications for the use of glycerol is its bioconversion to high value-added compounds, such as 1,3-propanediol (1,3-pd), succinate, ethanol, propionate, and hydrogen, through microbial fermentation. another method of waste material utilization is the application of crude glycerol in blends with other wastes (e.g., tomato waste hydrolysate). however, crude glycerol, a by-product of bi ... | 2014 | 25100926 |
| selection of specific endophytic bacterial genotypes by plants in response to soil contamination. | plant-bacterial combinations can increase contaminant degradation in the rhizosphere, but the role played by indigenous root-associated bacteria during plant growth in contaminated soils is unclear. the purpose of this study was to determine if plants had the ability to selectively enhance the prevalence of endophytes containing pollutant catabolic genes in unrelated environments contaminated with different pollutants. at petroleum hydrocarbon contaminated sites, two genes encoding hydrocarbon d ... | 2001 | 11375152 |
| positively regulated bacterial expression systems. | regulated promoters are useful tools for many aspects related to recombinant gene expression in bacteria, including for high-level expression of heterologous proteins and for expression at physiological levels in metabolic engineering applications. in general, it is common to express the genes of interest from an inducible promoter controlled either by a positive regulator or by a repressor protein. in this review, we discuss established and potentially useful positively regulated bacterial prom ... | 2008 | 21261879 |
| positively regulated bacterial expression systems. | regulated promoters are useful tools for many aspects related to recombinant gene expression in bacteria, including for high-level expression of heterologous proteins and for expression at physiological levels in metabolic engineering applications. in general, it is common to express the genes of interest from an inducible promoter controlled either by a positive regulator or by a repressor protein. in this review, we discuss established and potentially useful positively regulated bacterial prom ... | 2008 | 21261879 |
| bacterial biosensors for measuring availability of environmental pollutants. | traditionally, pollution risk assessment is based on the measurement of a pollutant's total concentration in a sample. the toxicity of a given pollutant in the environment, however, is tightly linked to its bioavailability, which may differ significantly from the total amount. physico-chemical and biological parameters strongly influence pollutant fate in terms of leaching, sequestration and biodegradation. bacterial sensorreporters, which consist of living micro-organisms genetically engineered ... | 2008 | 27879922 |
| cloning and characterisation of (r)-3-hydroxyacyl-acyl carrier protein-coenzyme a transferase gene (phag) from pseudomonas sp. usm 4-55. | the (r)-3-hydroxyacyl-acp-coa transferase catalyses the conversion of (r)-3-hydroxyacyl-acp to (r)-3-hydroxyacyl-coa derivatives, which serves as the ultimate precursor for polyhydroxyalkanoate (pha) polymerisation from unrelated substrates in pseudomonads. phag was found to be responsible for channelling precursors for polyhydroxyalkanoate (pha) synthase from a de novo fatty acid biosynthesis pathway when cultured on carbohydrates, such as glucose or gluconate. the phag gene was cloned from pse ... | 2009 | 24575175 |
| the pha depolymerase engineering database: a systematic analysis tool for the diverse family of polyhydroxyalkanoate (pha) depolymerases. | polyhydroxyalkanoates (phas) can be degraded by many microorganisms using intra- or extracellular pha depolymerases. pha depolymerases are very diverse in sequence and substrate specificity, but share a common alpha/beta-hydrolase fold and a catalytic triad, which is also found in other alpha/beta-hydrolases. | 2009 | 19296857 |
| advances in utilization of renewable substrates for biosurfactant production. | biosurfactants are amphiphilic molecules that have both hydrophilic and hydrophobic moieties which partition preferentially at the interfaces such as liquid/liquid, gas/liquid or solid/liquid interfaces. such characteristics enable emulsifying, foaming, detergency and dispersing properties. their low toxicity and environmental friendly nature and the wide range of potential industrial applications in bioremediation, health care, oil and food processing industries makes them a highly sought after ... | 2011 | 21906330 |
| host-microbe interactions in caenorhabditis elegans. | a good understanding of how microbes interact with hosts has a direct bearing on our capability of fighting infectious microbial pathogens and making good use of beneficial ones. among the model organisms used to study reciprocal actions among microbes and hosts, c. elegans may be the most advantageous in the context of its unique attributes such as the short life cycle, easiness of laboratory maintenance, and the availability of different genetic mutants. this review summarizes the recent advan ... | 2013 | 23984180 |
| structural insights into diversity and n-alkane biodegradation mechanisms of alkane hydroxylases. | environmental microbes utilize four degradation pathways for the oxidation of n-alkanes. although the enzymes degrading n-alkanes in different microbes may vary, enzymes functioning in the first step in the aerobic degradation of alkanes all belong to the alkane hydroxylases. alkane hydroxylases are a class of enzymes that insert oxygen atoms derived from molecular oxygen into different sites of the alkane terminus (or termini) depending on the type of enzymes. in this review, we summarize the d ... | 2013 | 23519435 |
| gacs-dependent regulation of polyhydroxyalkanoate synthesis in pseudomonas putida ca-3. | to date, limited reports are available on the regulatory systems exerting control over bacterial synthesis of the biodegradable polyester group known as polyhydroxyalkanoates (phas). in this study, we performed random mini-tn5 mutagenesis of the pseudomonas putida ca-3 genome and screened transconjugants on nitrogen-limited medium for reduced pha accumulation phenotypes. disruption of a gacs sensor kinase in one such mutant was found to eliminate medium-chain-length pha production in pseudomonas ... | 2013 | 23291549 |
| association with soil bacteria enhances p38-dependent infection resistance in caenorhabditis elegans. | the importance of our inner microbial communities for proper immune responses against invading pathogens is now well accepted, but the mechanisms underlying this protection are largely unknown. in this study, we used caenorhabditis elegans to investigate such mechanisms. since very little is known about the microbes interacting with c. elegans in its natural environment, we began by taking the first steps to characterize the c. elegans microbiota. we established a natural-like environment in whi ... | 2013 | 23230286 |
| an enhancer mutant of arabidopsis salt overly sensitive 3 mediates both ion homeostasis and the oxidative stress response. | the myristoylated calcium sensor sos3 and its interacting protein kinase, sos2, play critical regulatory roles in salt tolerance. mutations in either of these proteins render arabidopsis thaliana plants hypersensitive to salt stress. we report here the isolation and characterization of a mutant called enh1-1 that enhances the salt sensitivity of sos3-1 and also causes increased salt sensitivity by itself. enh1 encodes a chloroplast-localized protein with a pdz domain at the n-terminal region and ... | 2007 | 17485445 |
| metabolic network modeling of microbial interactions in natural and engineered environmental systems. | we review approaches to characterize metabolic interactions within microbial communities using stoichiometric metabolic network (smn) models for applications in environmental and industrial biotechnology. smn models are computational tools used to evaluate the metabolic engineering potential of various organisms. they have successfully been applied to design and optimize the microbial production of antibiotics, alcohols and amino acids by single strains. to date however, such models have been ra ... | 2016 | 27242701 |
| oprg harnesses the dynamics of its extracellular loops to transport small amino acids across the outer membrane of pseudomonas aeruginosa. | oprg is an outer membrane protein of pseudomonas aeruginosa whose function as an antibiotic-sensitive porin has been controversial and not well defined. circumstantial evidence led to the proposal that oprg might transport hydrophobic compounds by using a lateral gate in the barrel wall thought to be lined by three conserved prolines. to test this hypothesis and to find the physiological substrates of oprg, we reconstituted the purified protein into liposomes and found it to facilitate the trans ... | 2015 | 26655471 |
| effects of nacl concentration on anode microbes in microbial fuel cells. | understanding of how operational parameters affect the composition of exoelectrogenic microbes is an important step in the development of efficient microbial fuel cells (mfcs). in the present study, single-chamber mfcs were inoculated with rice paddy-field soil and continuously supplied with an acetate medium containing different concentrations of nacl (0-1.8 m). polarization analyses showed that power output increased as the nacl concentration increased to 0.1 m, while it was markedly diminishe ... | 2015 | 26061773 |
| bioenergetics and the role of soluble cytochromes c for alkaline adaptation in gram-negative alkaliphilic pseudomonas. | very few studies have been conducted on alkaline adaptation of gram-negative alkaliphiles. the reversed difference of h(+) concentration across the membrane will make energy production considerably difficult for gram-negative as well as gram-positive bacteria. cells of the alkaliphilic gram-negative bacterium pseudomonas alcaliphila al15-21(t) grown at ph 10 under low-aeration intensity have a soluble cytochrome c content that is 3.6-fold higher than that of the cells grown at ph 7 under high-ae ... | 2015 | 25705691 |
| biofilm as a production platform for heterologous production of rhamnolipids by the non-pathogenic strain pseudomonas putida kt2440. | although a transition toward sustainable production of chemicals is needed, the physiochemical properties of certain biochemicals such as biosurfactants make them challenging to produce in conventional bioreactor systems. alternative production platforms such as surface-attached biofilm populations could potentially overcome these challenges. rhamnolipids are a group of biosurfactants highly relevant for industrial applications. however, they are mainly produced by the opportunistic pathogen pse ... | 2016 | 27776509 |
| molecular cloning and functional expression of a δ9- fatty acid desaturase from an antarctic pseudomonas sp. a3. | fatty acid desaturase enzymes play an essential role in the synthesis of unsaturated fatty acids. pseudomonas sp. a3 was found to produce a large amount of palmitoleic and oleic acids after incubation at low temperatures. using polymerase chain reaction (pcr), a novel δ9- fatty acid desaturase gene was isolated, cloned, and successfully expressed in escherichia coli. the gene was designated as pa3fad9 and has an open reading frame of 1,185 bp which codes for 394 amino acids with a predicted mole ... | 2016 | 27494717 |
| gcsr, a tyrr-like enhancer-binding protein, regulates expression of the glycine cleavage system in pseudomonas aeruginosa pao1. | glycine serves as a major source of single carbon units for biochemical reactions within bacterial cells. utilization of glycine is tightly regulated and revolves around a key group of proteins known as the glycine cleavage system (gcs). our lab previously identified the transcriptional regulator gcsr (pa2449) as being required for catabolism of glycine in the opportunistic pathogen pseudomonas aeruginosa pao1. in an effort to clarify and have an overall better understanding of the role of gcsr ... | 2016 | 27303730 |
| metallic copper as an antimicrobial surface. | bacteria, yeasts, and viruses are rapidly killed on metallic copper surfaces, and the term "contact killing" has been coined for this process. while the phenomenon was already known in ancient times, it is currently receiving renewed attention. this is due to the potential use of copper as an antibacterial material in health care settings. contact killing was observed to take place at a rate of at least 7 to 8 logs per hour, and no live microorganisms were generally recovered from copper surface ... | 2010 | 21193661 |
| genotypic and phenotypic responses of a riverine microbial community to polycyclic aromatic hydrocarbon contamination. | the phenotypic and genotypic adaptation of a freshwater sedimentary microbial community to elevated (22 to 217 microgram [dry weight] of sediment-1) levels of polycyclic aromatic hydrocarbons (pahs) was determined by using an integrated biomolecular approach. central to the approach was the use of phospholipid fatty acid (plfa) profiles to characterize the microbial community structure and nucleic acid analysis to quantify the frequency of degradative genes. the study site was the little scioto ... | 1998 | 9726892 |
| microbial phyllosphere populations are more complex than previously realized. | phyllosphere microbial communities were evaluated on leaves of field-grown plant species by culture-dependent and -independent methods. denaturing gradient gel electrophoresis (dgge) with 16s rdna primers generally indicated that microbial community structures were similar on different individuals of the same plant species, but unique on different plant species. phyllosphere bacteria were identified from citrus sinesis (cv. valencia) by using dgge analysis followed by cloning and sequencing of t ... | 2001 | 11274410 |
| metallic copper as an antimicrobial surface. | bacteria, yeasts, and viruses are rapidly killed on metallic copper surfaces, and the term "contact killing" has been coined for this process. while the phenomenon was already known in ancient times, it is currently receiving renewed attention. this is due to the potential use of copper as an antibacterial material in health care settings. contact killing was observed to take place at a rate of at least 7 to 8 logs per hour, and no live microorganisms were generally recovered from copper surface ... | 2010 | 21193661 |
| a side by side comparison of bruker biotyper and vitek ms: utility of maldi-tof ms technology for microorganism identification in a public health reference laboratory. | matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) has emerged as a rapid, highly accurate, and cost-effective method for routine identification of a wide range of microorganisms. we carried out a side by side comparative evaluation of the performance of bruker biotyper versus vitek ms for identification of a large and diverse collection of microorganisms. most difficult and/or unusual microorganisms, as well as commonly encountered microorganisms were se ... | 2015 | 26658918 |
| linear array of conserved sequence motifs to discriminate protein subfamilies: study on pyridine nucleotide-disulfide reductases. | the pyridine nucleotide disulfide reductase (pndr) is a large and heterogeneous protein family divided into two classes (i and ii), which reflect the divergent evolution of its characteristic disulfide redox active site. however, not all the pndr members fit into these categories and this suggests the need of further studies to achieve a more comprehensive classification of this complex family. | 2007 | 17367536 |
| desaturases: emerging models for understanding functional diversification of diiron-containing enzymes. | desaturases and related enzymes perform o(2)-dependent dehydrogenations initiated at unactivated c-h groups with the use of a diiron active site. determination of the long-sought oxidized desaturase crystal structure facilitated structural comparison of the active sites of disparate diiron enzymes. experiments on the castor desaturase are discussed that provide experimental support for a hypothesized ancestral oxidase enzyme in the context of the evolution of the diiron enzyme diverse functional ... | 2009 | 19363032 |
| coupled chemo(enzymatic) reactions in continuous flow. | this review highlights the state of the art in the field of coupled chemo(enzymatic) reactions in continuous flow. three different approaches to such reaction systems are presented herein and discussed in view of their advantages and disadvantages as well as trends for their future development. | 2011 | 22238518 |
| differential expression of in vivo and in vitro protein profile of outer membrane of acidovorax avenae subsp. avenae. | outer membrane (om) proteins play a significant role in bacterial pathogenesis. in this work, we examined and compared the expression of the om proteins of the rice pathogen acidovorax avenae subsp. avenae strain rs-1, a gram-negative bacterium, both in an in vitro culture medium and in vivo rice plants. global proteomic profiling of a. avenae subsp. avenae strain rs-1 comparing in vivo and in vitro conditions revealed the differential expression of proteins affecting the survival and pathogenic ... | 2012 | 23166741 |
| engineering escherichia coli for production of c₁₂-c₁₄ polyhydroxyalkanoate from glucose. | demand for sustainable materials motivates the development of microorganisms capable of synthesizing products from renewable substrates. a challenge to commercial production of polyhydroxyalkanoates (pha), microbially derived polyesters, is engineering metabolic pathways to produce a polymer with the desired monomer composition from an unrelated and renewable source. here, we demonstrate a metabolic pathway for converting glucose into medium-chain-length (mcl)-pha composed primarily of 3-hydroxy ... | 2012 | 23141473 |
| whole-genome microarray and gene deletion studies reveal regulation of the polyhydroxyalkanoate production cycle by the stringent response in ralstonia eutropha h16. | poly(3-hydroxybutyrate) (phb) production and mobilization in ralstonia eutropha are well studied, but in only a few instances has phb production been explored in relation to other cellular processes. we examined the global gene expression of wild-type r. eutropha throughout the phb cycle: growth on fructose, phb production using fructose following ammonium depletion, and phb utilization in the absence of exogenous carbon after ammonium was resupplied. our results confirm or lend support to previ ... | 2012 | 22961894 |
| role of genetic redundancy in polyhydroxyalkanoate (pha) polymerases in pha biosynthesis in rhodospirillum rubrum. | this study investigated the apparent genetic redundancy in the biosynthesis of polyhydroxyalkanoates (phas) in the rhodospirillum rubrum genome revealed by the occurrence of three homologous pha polymerase genes (phac1, phac2, and phac3). in vitro biochemical assays established that each gene product encodes pha polymerase. a series of single, double, and triple phac deletion mutants were characterized with respect to pha production and growth capabilities on acetate or hexanoate as the sole car ... | 2012 | 22865850 |
| gmca is a putative glucose-methanol-choline oxidoreductase required for the induction of asexual development in aspergillus nidulans. | aspergillus nidulans asexual differentiation is induced by upstream developmental activators (udas) that include the bzip-type transcription factor (tf) flbb. a 2d-page/ms-ms-coupled screen for proteins differentially expressed in the presence and absence of flbb identified 18 candidates. most candidates belong to go term classes involved in osmotic and/or oxidative stress response. among these, we focused on gmca, a putative glucose-methanol-choline oxidoreductase which is upregulated in a δflb ... | 2012 | 22792266 |
| characterization of an extracellular polysaccharide produced by a pseudomonas strain grown on glycerol. | a new extracellular charged polysaccharide composed mainly by galactose, with lower amounts of mannose, glucose and rhamnose, was produced by the cultivation of pseudomonas oleovorans nrrl b-14682 using glycerol as the sole carbon source. thermal and solid-state nmr analysis showed that this polymer is essentially amorphous, with a glass transition temperature of 155.7 degrees c. the exopolysaccharide aqueous solutions have viscoelastic properties similar to that of guar gum, but with affinity t ... | 2009 | 18713662 |
| isolation of environmental bacteria from surface and drinking water in mafikeng, south africa, and characterization using their antibiotic resistance profiles. | the aim of this study was to isolate and identify environmental bacteria from various raw water sources as well as the drinking water distributions system in mafikeng, south africa, and to determine their antibiotic resistance profiles. water samples from five different sites (raw and drinking water) were analysed for the presence of faecal indicator bacteria as well as aeromonas and pseudomonas species. faecal and total coliforms were detected in summer in the treated water samples from the mod ... | 2014 | 25105027 |
| alkane inducible proteins in geobacillus thermoleovorans b23. | initial step of beta-oxidation is catalyzed by acyl-coa dehydrogenase in prokaryotes and mitochondria, while acyl-coa oxidase primarily functions in the peroxisomes of eukaryotes. oxidase reaction accompanies emission of toxic by-product reactive oxygen molecules including superoxide anion, and superoxide dismutase and catalase activities are essential to detoxify them in the peroxisomes. although there is an argument about whether primitive life was born and evolved under high temperature condi ... | 2009 | 19320977 |
| pseudomonas sp. as a source of medium chain length polyhydroxyalkanoates for controlled drug delivery: perspective. | controlled drug delivery technology represents one of the most rapidly advancing areas of science. they offer numerous advantages compared to conventional dosage forms including improved efficacy, reduced toxicity, improved patient compliance and convenience. over the past several decades, many delivery tools or methods were developed such as viral vector, liposome-based delivery system, polymer-based delivery system, and intelligent delivery system. recently, nonviral vectors, especially those ... | 2012 | 22518140 |
| whole cell biotransformation for reductive amination reactions. | whole cell biotransformation systems with enzyme cascading increasingly find application in biocatalysis to complement or replace established chemical synthetic routes for production of, e.g., fine chemicals. recently, we established an escherichia coli whole cell biotransformation system for reductive amination by coupling a transaminase and an amino acid dehydrogenase with glucose catabolism for cofactor recycling. transformation of 2-keto-3-methylvalerate to l-isoleucine by e. coli cells was ... | 2013 | 24406456 |
| whole cell biotransformation for reductive amination reactions. | whole cell biotransformation systems with enzyme cascading increasingly find application in biocatalysis to complement or replace established chemical synthetic routes for production of, e.g., fine chemicals. recently, we established an escherichia coli whole cell biotransformation system for reductive amination by coupling a transaminase and an amino acid dehydrogenase with glucose catabolism for cofactor recycling. transformation of 2-keto-3-methylvalerate to l-isoleucine by e. coli cells was ... | 2013 | 24406456 |
| ω3 fatty acid desaturases from microorganisms: structure, function, evolution, and biotechnological use. | the biosynthesis of very-long-chain polyunsaturated fatty acids involves an alternating process of fatty acid desaturation and elongation catalyzed by complex series of enzymes. ω3 desaturase plays an important role in converting ω6 fatty acids into ω3 fatty acids. genes for this desaturase have been identified and characterized in a wide range of microorganisms, including cyanobacteria, yeasts, molds, and microalgae. like all fatty acid desaturases, ω3 desaturase is structurally characterized b ... | 2013 | 24177732 |
| in vivo application of poly-3-hydroxyoctanoate as peripheral nerve graft. | this study aims to investigate the degree of biocompatibility and neuroregeneration of a polymer tube, poly-3-hydroxyoctanoate (pho) in nerve gap repair. | 2013 | 24190445 |
| radioimmunotherapy of cryptococcus neoformans spares bystander mammalian cells. | previously, we showed that radioimmunotherapy (rit) for cryptococcal infections using radioactively labeled antibodies recognizing the cryptococcal capsule reduced fungal burden and prolonged survival of mice infected with cryptococcus neoformans. here, we investigate the effects of rit on bystander mammalian cells. | 2013 | 24020737 |
| advances in the applications of polyhydroxyalkanoate nanoparticles for novel drug delivery system. | drug delivery technology is emerging as an interdisciplinary science aimed at improving human health. the controlled delivery of pharmacologically active agents to the specific site of action at the therapeutically optimal rate and dose regimen has been a major goal in designing drug delivery systems. over the past few decades, there has been considerable interest in developing biodegradable drug carriers as effective drug delivery systems. polymeric materials from natural sources play an import ... | 2013 | 23984383 |
| analysis of two polyhydroxyalkanoate synthases in bradyrhizobium japonicum usda 110. | bradyrhizobium japonicum usda 110 has five polyhydroxyalkanoate (pha) synthases (phac) annotated in its genome: bll4360 (phac1), bll6073 (phac2), blr3732 (phac3), blr2885 (phac4), and bll4548 (phac5). all these proteins possess the catalytic triad and conserved amino acid residues of polyester synthases and are distributed into four different phac classes. we obtained mutants in each of these paralogs and analyzed phac gene expression and pha production in liquid cultures. despite the genetic re ... | 2013 | 23667236 |
| characterization of site-specific mutations in a short-chain-length/medium-chain-length polyhydroxyalkanoate synthase: in vivo and in vitro studies of enzymatic activity and substrate specificity. | saturation point mutagenesis was carried out at position 479 in the polyhydroxyalkanoate (pha) synthase from chromobacterium sp. strain usm2 (phac(cs)) with specificities for short-chain-length (scl) [(r)-3-hydroxybutyrate (3hb) and (r)-3-hydroxyvalerate (3hv)] and medium-chain-length (mcl) [(r)-3-hydroxyhexanoate (3hhx)] monomers in an effort to enhance the specificity of the enzyme for 3hhx. a maximum 4-fold increase in 3hhx incorporation and a 1.6-fold increase in pha biosynthesis, more than ... | 2013 | 23584780 |
| symptoms of fern distortion syndrome resulting from inoculation with opportunistic endophytic fluorescent pseudomonas spp. | fern distortion syndrome (fds) is a serious disease of leatherleaf fern (rumohra adiantiformis). the main symptom of fds is distortion of fronds, making them unmarketable. additional symptoms include stunting, irregular sporulation, decreased rhizome diameter, and internal discoloration of rhizomes. we previously reported an association of symptoms with increased endophytic rhizome populations of fluorescent pseudomonads (fps). the aim of the current study was to determine if fps from ferns in c ... | 2013 | 23516499 |
| efficient production of active polyhydroxyalkanoate synthase in escherichia coli by coexpression of molecular chaperones. | the type i polyhydroxyalkanoate synthase from cupriavidus necator was heterologously expressed in escherichia coli with simultaneous overexpression of chaperone proteins. compared to expression of synthase alone (14.55 mg liter(-1)), coexpression with chaperones resulted in the production of larger total quantities of enzyme, including a larger proportion in the soluble fraction. the largest increase was seen when the groel/groes system was coexpressed, resulting in approximately 6-fold-greater ... | 2013 | 23335776 |
| biodegradation kinetics of tetrahydrofuran, benzene, toluene, and ethylbenzene as multi-substrate by pseudomonas oleovorans dt4. | the biodegradation kinetics of tetrahydrofuran, benzene (b), toluene (t), and ethylbenzene (e) were systematically investigated individually and as mixtures by a series of aerobic batch degradation experiments initiated by pseudomonas oleovorans dt4. the andrews model parameters, e.g., maximum specific growth rates (μmax), half saturation, and substrate inhibition constant, were obtained from single-substrate experiments. the interaction parameters in the sum kinetics model (skip) were obtained ... | 2014 | 25561017 |
| biodegradation kinetics of tetrahydrofuran, benzene, toluene, and ethylbenzene as multi-substrate by pseudomonas oleovorans dt4. | the biodegradation kinetics of tetrahydrofuran, benzene (b), toluene (t), and ethylbenzene (e) were systematically investigated individually and as mixtures by a series of aerobic batch degradation experiments initiated by pseudomonas oleovorans dt4. the andrews model parameters, e.g., maximum specific growth rates (μmax), half saturation, and substrate inhibition constant, were obtained from single-substrate experiments. the interaction parameters in the sum kinetics model (skip) were obtained ... | 2014 | 25561017 |
| the near-quantitative sampling of genomic dna from various food-borne eubacteria. | the disruption of the bacterial cell wall plays an important part in achieving quantitative extraction of dna from eubacteria essential for accurate analyses of genetic material recovered from environmental samples. | 2014 | 25551371 |
| pseudomonas putida fis binds to the lapf promoter in vitro and represses the expression of lapf. | the biofilm matrix of the rhizospheric bacterium pseudomonas putida consists mainly of a proteinaceous component. the two largest p. putida proteins, adhesins lapa and lapf, are involved in biofilm development but prevail in different developmental stages of the biofilm matrix. lapa is abundant in the initial stage of biofilm formation whereas lapf is found in the mature biofilm. although the transcriptional regulation of the adhesins is not exhaustively studied, some factors that can be involve ... | 2014 | 25545773 |
| paclitaxel-incorporated nanoparticles using block copolymers composed of poly(ethylene glycol)/poly(3-hydroxyoctanoate). | block copolymers composed of poly(3-hydroxyoctanoate) (pho) and methoxy poly(ethylene glycol) (peg) were synthesized to prepare paclitaxel-incorporated nanoparticle for antitumor drug delivery. in a (1)h-nmr study, chemical structures of pho/peg block copolymers were confirmed and their molecular weight (m.w.) was analyzed with gel permeation chromatography (gpc). paclitaxel as a model anticancer drug was incorporated into the nanoparticles of pho/peg block copolymer. they have spherical shapes ... | 2014 | 25288916 |
| response mechanisms of bacterial degraders to environmental contaminants on the level of cell walls and cytoplasmic membrane. | bacterial strains living in the environment must cope with the toxic compounds originating from humans production. surface bacterial structures, cell wall and cytoplasmic membrane, surround each bacterial cell and create selective barriers between the cell interior and the outside world. they are a first site of contact between the cell and toxic compounds. organic pollutants are able to penetrate into cytoplasmic membrane and affect membrane physiological functions. bacteria had to evolve adapt ... | 2014 | 25057269 |
| biocatalytic anti-prelog reduction of prochiral ketones with whole cells of acetobacter pasteurianus gim1.158. | enantiomerically pure alcohols are important building blocks for production of chiral pharmaceuticals, flavors, agrochemicals and functional materials and appropriate whole-cell biocatalysts offer a highly enantioselective, minimally polluting route to these valuable compounds. at present, most of these biocatalysts follow prelog's rule, and thus the (s)-alcohols are usually obtained when the smaller substituent of the ketone has the lower cip priority. only a few anti-prelog (r)-specific whole ... | 2014 | 24916156 |
| evaluating pha productivity of bioengineered rhodosprillum rubrum. | this study explored the potential of using rhodosprillum rubrum as the biological vehicle to convert chemically simple carbon precursors to a value-added bio-based product, the biopolymer pha. r. rubrum strains were bioengineered to overexpress individually or in various combinations, six pha biosynthetic genes (phac1, phaa, phab, phac2, phac3, and phaj), and the resulting nine over-expressing strains were evaluated to assess the effect on pha content, and the effect on growth. these experiments ... | 2014 | 24840941 |
| scale-up of anaerobic 1,3-propanediol production by clostridium butyricum dsp1 from crude glycerol. | as the production of biofuels from raw materials continuously increases, optimization of production processes is necessary. a very important issue is the development of wasteless methods of biodiesel production. one way of utilization of glycerol generated in biodiesel production is its microbial conversion to 1,3-pd (1,3-propanediol). | 2014 | 24555775 |
| detection of organic compounds with whole-cell bioluminescent bioassays. | natural and manmade organic chemicals are widely deposited across a diverse range of ecosystems including air, surface water, groundwater, wastewater, soil, sediment, and marine environments. some organic compounds, despite their industrial values, are toxic to living organisms and pose significant health risks to humans and wildlife. detection and monitoring of these organic pollutants in environmental matrices therefore is of great interest and need for remediation and health risk assessment. ... | 2014 | 25084996 |
| microbial diversity and activity in the nematostella vectensis holobiont: insights from 16s rrna gene sequencing, isolate genomes, and a pilot-scale survey of gene expression. | we have characterized the molecular and genomic diversity of the microbiota of the starlet sea anemone nematostella vectensis, a cnidarian model for comparative developmental and functional biology and a year-round inhabitant of temperate salt marshes. molecular phylogenetic analysis of 16s rrna gene clone libraries revealed four ribotypes associated with n. vectensis at multiple locations and times. these associates include two novel ribotypes within the ε-proteobacterial order campylobacterale ... | 2015 | 26388838 |
| gpo1 alkb gene expression for improvement of the degradation of diesel oil by a bacterial consortium. | to facilitate the biodegradation of diesel oil, an oil biodegradation bacterial consortium was constructed. the alkane hydroxylase (alkb) gene of pseudomonas putida gpo1 was constructed in a pcom8 expression vector, and the pcom8-gpo1 alkb plasmid was transformed into escherichia coli dh5α. the alkb protein was expressed by diesel oil induction and detected through sds-polyacrylamide gel electrophoresis. the culture of the recombinant (pcom8-gpo1 alkb/e. coli dh5α) with the oil biodegradation ba ... | 2015 | 26413044 |
| "mentor in bioeconomics avant la lettre": a tribute to bernard witholt by those who worked with him. | | 2015 | 26074417 |
| synthesis of microbial elastomers based on soybean oily acids. biocompatibility studies. | biocompatibility studies of the autoxidized and unoxidized unsaturated medium-long chain length (m-lcl) co-poly-3-hydroxyalkanoates (m-lclphas) derived from soya oily acids have been reported. pseudomonas oleovorans was grown on a series of mixtures of octanoic acid (oa) and soya oily acids (sy) with weight ratios of 20:80, 28:72 and 50:50 in order to obtain unsaturated m-lcl copolyesters coded pho-sy-2080, pho-sy-2872 and pho-sy-5050, respectively. the pha films were obtained by solvent cast fr ... | 2009 | 19498224 |
| evaluation of antioxidant, antibacterial, and antidiabetic potential of two traditional medicinal plants of india: swertia cordata and swertia chirayita. | swertia cordata and swertia chirayita are temperate himalayan medicinal plants used as potent herbal drugs in indian traditional systems of medicine (ayurvedic, unani and siddha). | 2015 | 26109789 |
| root-associated bacterial endophytes from ralstonia solanacearum resistant and susceptible tomato cultivars and their pathogen antagonistic effects. | this study was undertaken to assess if the root-associated native bacterial endophytes in tomato have any bearing in governing the host resistance to the wilt pathogen ralstonia solanacearum. internal colonization of roots by bacterial endophytes was confirmed through confocal imaging after syto-9 staining. endophytes were isolated from surface-sterilized roots of 4-weeks-old seedlings of known wilt resistant (r) tomato cultivar arka abha and susceptible (s) cv. arka vikas on nutrient agar after ... | 2015 | 25926818 |
| a newly isolated strain capable of effectively degrading tetrahydrofuran and its performance in a continuous flow system. | a gram-negative strain dt4, capable of growing aerobically on tetrahydrofuran (thf) as the sole carbon and energy source was isolated from a pharmaceutical wastewater treatment plant. it was identified as pseudomonas oleovorans by morphological and physiological characteristics as well as biolog profiling and 16s rdna sequence. cells of p. oleovorans dt4 pre-cultured in thf could degrade 5 mm thf completely without lag phase. the generation time of 2.7 h and the maximum degradation rate of 203.9 ... | 2010 | 20381342 |
| evidence that the yeast desaturase ole1p exists as a dimer in vivo. | desaturase enzymes are composed of two classes, the structurally well characterized soluble class found predominantly in the plastids of higher plants and the more widely distributed but poorly structurally defined integral membrane class. despite their distinct evolutionary origins, the two classes both require an iron cofactor and molecular oxygen for activity and are inhibited by azide and cyanide, suggesting strong mechanistic similarities. the fact that the soluble desaturase is active as a ... | 2010 | 20406812 |
| cloning and expression of poly 3-hydroxybutyrate operon into escherichia coli. | poly 3-hydroxybutyrate (phb), a class of poly hydroxyalkanoates (phas), is a group of bacterial storage polymers, produced by various microorganisms in response to nutrient limitation. phas are biodegradable polymers which could be a good substitute for current petrochemical plastics. phb has been synthesized during three enzymatic steps including three genes. | 2015 | 25834710 |
| occurrence, diversity and community structure of culturable atrazine degraders in industrial and agricultural soils exposed to the herbicide in shandong province, p.r. china. | soil populations of bacteria rapidly degrading atrazine are critical to the environmental fate of the herbicide. an enrichment bias from the routine isolation procedure prevents studying the diversity of atrazine degraders. in the present work, we analyzed the occurrence, diversity and community structure of soil atrazine-degrading bacteria based on their direct isolation. | 2016 | 27821056 |
| editorial: harnessing useful rhizosphere microorganisms for pathogen and pest biocontrol. | | 2016 | 27807429 |
| species diversity of environmental gim-1-producing bacteria collected during a long-term outbreak. | reports of outbreaks concerning carbapenemase-producing gram-negative bacteria in which the main source of transmission is the hospital environment are increasing. this study describes the results of environmental sampling in a protracted polyspecies metallo-beta-lactamase gim-1 outbreak driven by plasmids and bacterial clones of enterobacter cloacae and pseudomonas aeruginosa in a tertiary care center. environmental sampling targeting wet locations (especially sinks) was carried out on a surgic ... | 2016 | 27060121 |
| biosynthesis and characterization of copolymer poly(3hb-co-3hv) from saponified jatropha curcas oil by pseudomonas oleovorans. | polyhydroxyalkanoates (phas) are naturally occurring biodegradable polymers with promising application in the formulation of plastic materials. phas are produced by numerous bacteria as energy/carbon storage materials from various substrates, including sugars and plant oils. since these substrates compete as food sources, their use as raw material for industrial-scale production of pha is limited. therefore, efforts have been focused on seeking alternative sources for bacterial production of pha ... | 2010 | 20467780 |
| reactive oxygen species and small-conductance calcium-dependent potassium channels are key mediators of inflammation-induced hypotension and shock. | septic shock is associated with life-threatening vasodilation and hypotension. to cause vasodilation, vascular endothelium may release nitric oxide (no), prostacyclin (pgi2), and the elusive endothelium-derived hyperpolarizing factor (edhf). although no is critical in controlling vascular tone, inhibiting no in septic shock does not improve outcome, on the contrary, precipitating the search for alternative therapeutic targets. using a hyperacute tumor necrosis factor (tnf)-induced shock model in ... | 2010 | 20496172 |
| pseudomonas toyotomiensis sp. nov., psychrotolerant facultative alkaliphile that utilizes hydrocarbons. | a psychrotolerant facultatively alkaliphilic strain, designated as strain ht-3t, was isolated from a sample of soil immersed in hot spring water containing hydrocarbons in toyotomi, hokkaido, japan. 16s rrna gene sequence phylogeny suggested that strain ht-3t is a member of the genus pseudomonas and belong to the pseudomonas oleovorans group. the cells of the isolate were gram-negative and aerobic straight rods that were motile by one polar flagellum. the strain grew between 4 and 42 degrees c w ... | 2010 | 20817837 |
| hydroxylated ornithine lipids increase stress tolerance in rhizobium tropici ciat899. | ornithine lipids (ols) are widespread among gram-negative bacteria. their basic structure consists of a 3-hydroxy fatty acyl group attached in amide linkage to the α-amino group of ornithine and a second fatty acyl group ester-linked to the 3-hydroxy position of the first fatty acid. ols can be hydroxylated within the secondary fatty acyl moiety and this modification has been related to increased stress tolerance. rhizobium tropici, a nodule-forming α-proteobacterium known for its stress toleran ... | 2011 | 21205018 |
| production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil by engineered ralstonia eutropha strains. | the polyhydroxyalkanoate (pha) copolymer poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [p(hb-co-hhx)] has been shown to have potential to serve as a commercial bioplastic. synthesis of p(hb-co-hhx) from plant oil has been demonstrated with recombinant ralstonia eutropha strains expressing heterologous pha synthases capable of incorporating hb and hhx into the polymer. with these strains, however, short-chain-length fatty acids had to be included in the medium to generate pha with high hhx conten ... | 2011 | 21398488 |
| [characteristics of tetrahydrofuran degradation by pseudomonas oleovorans dt4]. | a tetrahydrofuran (thf)-degrading strain pseudomonas oleovorans dt4 was isolated from the activated sludge of a pharmaceutical plant. p. oleovorans dt4 was able to utilize thf as the sole carbon and energy source under aerobic condition. 5 mmol/l of thf could be completely degraded by 3.2 mg/l inoculums of p. oleovorans dt4 in 14 h at ph 7.2 and 30 degrees c, with the cells concentration increasing to 188.6 mg/l. after the complete consumption of thf, no toc could be detected but ic reached the ... | 2011 | 21404697 |
| Genetic Diversity of Endophytic Diazotrophs of the Wild Rice, Oryza alta and Identification of the New Diazotroph, Acinetobacter oryzae sp. nov. | Thirty-three endophytic diazotrophs were isolated from surface-sterilized leaves, stem, and roots of wild rice Oryza alta. The SDS-PAGE profile of total protein and insertion sequence-based polymerase chain reaction (IS-PCR) fingerprinting grouped the isolates into four clusters (I-IV). The 16S rRNA gene sequence homology of the representative strains B21, B31, B1, and B23 of clusters I, II, III, and IV were assigned to Pseudomonas oleovorans (99.2% similarity), Burkholderia fungorum (99.4% simi ... | 2011 | 22105517 |
| value-added uses for crude glycerol--a byproduct of biodiesel production. | biodiesel is a promising alternative, and renewable, fuel. as its production increases, so does production of the principle co-product, crude glycerol. the effective utilization of crude glycerol will contribute to the viability of biodiesel. in this review, composition and quality factors of crude glycerol are discussed. the value-added utilization opportunities of crude glycerol are reviewed. the majority of crude glycerol is used as feedstock for production of other value-added chemicals, fol ... | 2012 | 22413907 |
| production of polyhydroxybutyrate and alginate from glycerol by azotobacter vinelandii under nitrogen-free conditions. | glycerol is an interesting feedstock for biomaterials such as biofuels and bioplastics because of its abundance as a by-product during biodiesel production. here we demonstrate glycerol metabolism in the nitrogen-fixing species azotobacter vinelandii through metabolomics and nitrogen-free bacterial production of biopolymers, such as poly-d-3-hydroxybutyrate (phb) and alginate, from glycerol. glycerol-3-phosphate was accumulated in a. vinelandii cells grown on glycerol to the exponential phase, a ... | 2015 | 25880041 |
| genome scale prediction of substrate specificity for acyl adenylate superfamily of enzymes based on active site residue profiles. | enzymes belonging to acyl:coa synthetase (acs) superfamily activate wide variety of substrates and play major role in increasing the structural and functional diversity of various secondary metabolites in microbes and plants. however, due to the large sequence divergence within the superfamily, it is difficult to predict their substrate preference by annotation transfer from the closest homolog. therefore, a large number of acs sequences present in public databases lack any functional annotation ... | 2010 | 20105319 |
| initial reactions in anaerobic alkane degradation by a sulfate reducer, strain ak-01. | an alkane-degrading, sulfate-reducing bacterial strain, ak-01, isolated from a petroleum-contaminated sediment was studied to elucidate its mechanism of alkane metabolism. total cellular fatty acids of ak-01 were predominantly c even when it was grown on c-even alkanes and were predominantly c odd when grown on c-odd alkanes, suggesting that the bacterium anaerobically oxidizes alkanes to fatty acids. among these fatty acids, some 2-, 4-, and 6-methylated fatty acids were specifically found only ... | 1999 | 10584014 |
| decolourisation of different dyes by two pseudomonas strains under various growth conditions. | the aim of the present study was the decolourisation of mixture of two dyes belonging to different groups by two pseudomonas fluorescens strains (sz6 and sdz3). influence of different incubation conditions on decolourisation effectiveness was evaluated. dyes used in the experiment were diazo evans blue (eb) and triphenylmethane brilliant green (bg). another goal of the experiment was the estimation of toxicity of process by-products. incubation conditions had a significant influence on the rate ... | 2014 | 24578585 |
| isolation and screening of polyhydroxyalkanoates producing bacteria from pulp, paper, and cardboard industry wastes. | background. polyhydroxyalkanoates (phas) are storage materials that accumulate by various bacteria as energy and carbon reserve materials. they are biodegradable, environmentally friendly, and also biocompatible bioplastics. unlike petrochemical-based plastics that take several decades to fully degrade, phas can be completely degraded within a year by variety of microorganisms into co2 and water. in the present study, we aim to utilize pulp, paper, and cardboard industry sludge and waste water f ... | 2013 | 24288534 |
| bacterial metabolism of methylated amines and identification of novel methylotrophs in movile cave. | movile cave, romania, is an unusual underground ecosystem that has been sealed off from the outside world for several million years and is sustained by non-phototrophic carbon fixation. methane and sulfur-oxidising bacteria are the main primary producers, supporting a complex food web that includes bacteria, fungi and cave-adapted invertebrates. a range of methylotrophic bacteria in movile cave grow on one-carbon compounds including methylated amines, which are produced via decomposition of orga ... | 2014 | 25050523 |
| bacterial metabolism of methylated amines and identification of novel methylotrophs in movile cave. | movile cave, romania, is an unusual underground ecosystem that has been sealed off from the outside world for several million years and is sustained by non-phototrophic carbon fixation. methane and sulfur-oxidising bacteria are the main primary producers, supporting a complex food web that includes bacteria, fungi and cave-adapted invertebrates. a range of methylotrophic bacteria in movile cave grow on one-carbon compounds including methylated amines, which are produced via decomposition of orga ... | 2014 | 25050523 |