| characterization of pseudomonas spp. associated with spoilage of gilt-head sea bream stored under various conditions. | the population dynamics of pseudomonads in gilt-head sea bream mediterranean fish (sparus aurata) stored under different conditions were studied. phenotypic analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins were performed to identify a total of 106 pseudomonas strains isolated from s. aurata stored under different temperatures (at 0, 10, and 20 degrees c) and packaging conditions (air and a modified atmosphere of 40% co(2)-30% n(2)-30% o(2)). pseudomon ... | 2002 | 11772610 |
| new degenerate cytophaga-flexibacter-bacteroides-specific 16s ribosomal dna-targeted oligonucleotide probes reveal high bacterial diversity in river taff epilithon. | river microbial communities play an important role in global nutrient cycles, and aggregated bacteria such as those in epilithic biofilms may be major contributors. in this study the bacterial diversity of river taff epilithon in south wales was investigated. a 16s ribosomal dna (rdna) clone library was constructed and analyzed by partial sequencing of 76 of 347 clones and hybridization with taxon-specific probes. the epilithon was found to be very diverse, with an estimated 59.6% of the bacteri ... | 2002 | 11772628 |
| salt stress proteins induced in listeria monocytogenes. | the ability of listeria monocytogenes to tolerate salt stress is of particular importance, as this pathogen is often exposed to such environments during both food processing and food preservation. in order to understand the survival mechanisms of l. monocytogenes, an initial approach using two-dimensional polyacrylamide gel electrophoresis was performed to analyze the pattern of protein synthesis in response to salt stress. of 400 to 500 visible proteins, the synthesis of 40 proteins (p < 0.05) ... | 2002 | 11916660 |
| role of glucose in enhancing the temperature-dependent growth inhibition of escherichia coli o157:h7 atcc 43895 by a pseudomonas sp. | growth of escherichia coli o157:h7 strain atcc 43895 was monitored at 5, 10, 15, and 25 degrees c in both pure and mixed (1:1) cultures with a gluconate-producing pseudomonas sp. found in meat to evaluate the effect of the absence and presence of 1% glucose in broth on temperature-dependent competition. the number of colonies of the pseudomonas strain exceeded 9 log cfu/ml under all conditions tested. the pathogen grew better as the temperature increased from 10 to 15 and 25 degrees c and grew b ... | 2002 | 11976143 |
| a new inhibitor of mitochondrial fatty acid oxidation. | the mitochondrial enoyl-coa hydratase/3-hydroxyacyl-coa dehydrogenase/3-ketoacyl-coa thiolase trifunctional protein (trifunctional protein) plays a major role in mitochondrial fatty acid oxidation. the enzyme complex consists of four molecules of alpha-subunit containing both hydratase and dehydrogenase domains and four molecules of beta-subunit containing the thiolase domain. the primary structure of a gastrin-binding protein (gbp) was highly homologous to that of the alpha-subunit of the trifu ... | 1996 | 8827458 |
| axenic aerobic biofilms inhibit corrosion of sae 1018 steel through oxygen depletion. | corrosion inhibition of sae 1018 steel by pure-culture biofilms of pseudomonas fragi and escherichia coli dh5 alpha has been evaluated in complex luria-bertani medium, seawater-mimicking medium, and modified baar's medium at 30 degrees c. in batch cultures, both bacteria inhibited corrosion three to six fold compared to sterile controls, and the corrosion was comparable to that observed in anaerobic sterile media. to corroborate this result, a continuous reactor and electrochemical impedance spe ... | 1997 | 9274042 |
| the cold-active lipase of pseudomonas fragi. heterologous expression, biochemical characterization and molecular modeling. | a recombinant lipase cloned from pseudomonas fragi strain ifo 3458 (pfl) was found to retain significant activity at low temperature. in an attempt to elucidate the structural basis of this behaviour, a model of its three-dimensional structure was built by homology and compared with homologous mesophilic lipases, i.e. the pseudomonas aeruginosa lipase (45% sequence identity) and burkholderia cepacia lipase (38%). in this model, features common to all known lipases have been identified, such as t ... | 2002 | 12084074 |
| yfcx enables medium-chain-length poly(3-hydroxyalkanoate) formation from fatty acids in recombinant escherichia coli fadb strains. | expression of escherichia coli open reading frame yfcx is shown to be required for medium-chain-length polyhydroxyalkanoate (pha(mcl)) formation from fatty acids in an e. coli fadb mutant. the open reading frame encodes a protein, yfcx, with significant similarity to the large subunit of multifunctional beta-oxidation enzymes. e. coli fadb strains modified to contain an inactivated copy of yfcx and to express a medium-chain-length synthase are unable to form pha(mcl)s when grown in the presence ... | 2002 | 12270828 |
| control of pyrimidine synthesis in pseudomonas fragi. | to study the regulation of the de novo pyrimidine biosynthetic enzymes in the food-spoilage agent pseudomonas fragi atcc 4973. | 2002 | 12390485 |
| the gene for albicidin detoxification from pantoea dispersa encodes an esterase and attenuates pathogenicity of xanthomonas albilineans to sugarcane. | albicidin phytotoxins are pathogenicity factors in a devastating disease of sugarcane known as leaf scald, caused by xanthomonas albilineans. a gene (albd) from pantoea dispersa has been cloned and sequenced and been shown to code for a peptide of 235 amino acids that detoxifies albicidin. the gene shows no significant homology at the dna or protein level to any known sequence, but the gene product contains a gxsxg motif that is conserved in serine hydrolases. the albd protein, purified to homog ... | 1997 | 9275238 |
| nutrition and physiology of pseudomonas fragi. | | 1957 | 13502296 |
| taxonomic relationships among the pseudomonads. | colwell, r. r. (university of washington, seattle), and j. liston. taxonomic relationships among the pseudomonads. j. bacteriol. 82:1-14. 1961.-an electronic computer technique, utilizing the adansonian principle that every feature should have equal weight, was applied in an effort to derive a taxonomy of the pseudomonas-achromobacter group of gram-negative, asporogenous, rodlike bacteria. the validity of the general method was tested by an analysis of 40 well defined strains, principally derive ... | 1961 | 13694873 |
| the cold shock response of the psychrotrophic bacterium pseudomonas fragi involves four low-molecular-mass nucleic acid-binding proteins. | the psychrotrophic bacterium pseudomonas fragi was subjected to cold shocks from 30 or 20 to 5 degrees c. the downshifts were followed by a lag phase before growth resumed at a characteristic 5 degrees c growth rate. the analysis of protein patterns by two-dimentional gel electrophoresis revealed overexpression of 25 or 17 proteins and underexpression of 12 proteins following the 30- or 20-to-5 degrees c shift, respectively. the two downshifts shared similar variations of synthesis of 20 protein ... | 1997 | 9393697 |
| reconstitution, morphology and crystallization of a fatty acid beta-oxidation multienzyme complex from pseudomonas fragi. | the fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, hdt, exhibits predominantly the three enzymic activities of 2-enoyl-coa hydratase (ec 4.2.1.17), 3-hydroxyacyl-coa dehydrogenase (ec 1.1.1.35) and 3-oxoacyl-coa thiolase (ec 2.3.1.16). the hdt complex is encoded by the faoab operon, consisting of the faoa and faob genes that encode two individual constituents, the alpha-subunit and the beta-subunit. we have constructed escherichia coli overexpression systems for the faoab ... | 1997 | 9396725 |
| acyl coenzyme a synthetase from pseudomonas fragi catalyzes the synthesis of adenosine 5'-polyphosphates and dinucleoside polyphosphates. | acyl coenzyme a (coa) synthetase (ec 6.2.1.8) from pseudomonas fragi catalyzes the synthesis of adenosine 5'-tetraphosphate (p4a) and adenosine 5'-pentaphosphate (p5a) from atp and tri- or tetrapolyphosphate, respectively. datp, adenosine-5'-o-[gamma-thiotriphosphate] (atp gamma s), adenosine(5')tetraphospho(5')adenosine (ap4a), and adenosine(5')pentaphospho(5')adenosine (ap5a) are also substrates of the reaction yielding p4(d)a in the presence of tripolyphosphate (p3). utp, ctp, and amp are not ... | 1998 | 9620965 |
| characterization of axenic pseudomonas fragi and escherichia coli biofilms that inhibit corrosion of sae 1018 steel. | corrosion inhibition of sae 1018 steel by pseudomonas fragi and escherichia coli biofilms has been evaluated using batch cultures in rich medium (lb) and seawater-mimicking medium (vnss) at 23 degrees c and 30 degrees c with or without daily medium replenishment. biofilm components have been stained simultaneously for polysaccharide (calcofluor) and live and dead cells (live/dead baclit viability kit) and visualized using confocal scanning laser microscopy (cslm). image analysis was used to quan ... | 1998 | 9633647 |
| a sensitive assay of acyl-coenzyme a oxidase by coupling with beta-oxidation multienzyme complex. | a highly sensitive and reliable method for assaying acyl-coa oxidase (ec 1.3.99.3) activity was developed. an acyl-coa oxidase-dependent [1-14c]palmitoyl-coa degradation to acetyl-coa, acid-soluble products, was measured by coupling with the multienzyme complex for fatty acid oxidation from pseudomonas fragi. the activity, more than 2 pmol/min, could be assessed using this method. the activity was dependent on the coupling enzyme (multienzyme complex), coenzymes such as nad+ and coa, and oxygen, ... | 1994 | 7810878 |
| effect of growth temperatures on the protein levels in a psychrotrophic bacterium, pseudomonas fragi. | pseudomonas fragi has the ability to grow between 0 and 35 degrees c and grows optimally at 30 degrees c. cellular proteins from mid-log-phase cells growing from 4 to 34 degrees c were labeled with l-[35s]methionine during 1 generation time and analyzed by two-dimensional gel electrophoresis. the electrophoretic patterns revealed differences in the patterns of protein synthesis over this temperature span. a qualitative comparison of cellular proteins led to their separation into five thermal cla ... | 1994 | 8021184 |
| rapid and specific detection of salmonella spp. in animal feed samples by pcr after culture enrichment. | a pcr procedure has been developed for routine analysis of viable salmonella spp. in feed samples. the objective was to develop a simple pcr-compatible enrichment procedure to enable dna amplification without any sample pretreatment such as dna extraction or cell lysis. pcr inhibition by 14 different feed samples and natural background flora was circumvented by the use of the dna polymerase tth. this dna polymerase was found to exhibit a high level of resistance to pcr inhibitors present in thes ... | 2004 | 14711627 |
| continuous recording of long-chain acyl-coenzyme a synthetase activity using fluorescently labeled bovine serum albumin. | the fluorescence-based long-chain fatty acid probe bsa-hca (bovine serum albumin labeled with 7-hydroxycoumarin-4-acetic acid) is shown to respond to binding of long-chain acyl-coa thioesters by quenching of the 450 nm fluorescence emission. as determined by spectrofluorometric titration, binding affinities for palmitoyl-, stearoyl-, and oleoyl-coa (kd = 0.2-0.4 microm) are 5-10 times lower than those for the corresponding nonesterified fatty acids. in the presence of detergent (chaps, triton x- ... | 2001 | 11476543 |
| combined effects of nacl, naoh, and biocides (monolaurin or lauric acid) on inactivation of listeria monocytogenes and pseudomonas spp. | this study highlighted combinations of chemical stresses that could decrease or eliminate listeria monocytogenes and pseudomonas spp. surviving in food processing plants. strains of l. monocytogenes, pseudomonas fragi, and pseudomonas fluorescens isolated from processing environments (meat and milk) were grown at 20 degrees c up to the early stationary phase. the strains were then subjected to 30 min of physicochemical treatments. these treatments included individual or combined acid (acetic aci ... | 2001 | 11563526 |
| myeloperoxidase produces nitrating oxidants in vivo. | despite intense interest in pathways that generate reactive nitrogen species, the physiologically relevant mechanisms for inflammatory tissue injury remain poorly understood. one possible mediator is myeloperoxidase, a major constituent of neutrophils, monocytes, and some populations of macrophages. the enzyme uses hydrogen peroxide and nitrite to generate 3-nitrotyrosine in vitro. to determine whether myeloperoxidase produces nitrating intermediates in vivo, we used isotope dilution gas chromat ... | 2002 | 12021246 |
| microtiter plate assay for assessment of listeria monocytogenes biofilm formation. | listeria monocytogenes has the ability to form biofilms on food-processing surfaces, potentially leading to food product contamination. the objective of this research was to standardize a polyvinyl chloride (pvc) microtiter plate assay to compare the ability of l. monocytogenes strains to form biofilms. a total of 31 coded l. monocytogenes strains were grown in defined medium (modified welshimer's broth) at 32 degrees c for 20 and 40 h in pvc microtiter plate wells. biofilm formation was indirec ... | 2002 | 12039754 |
| modeling the lag time of listeria monocytogenes from viable count enumeration and optical density data. | the following two factors significantly influence estimates of the maximum specific growth rate ( micro (max)) and the lag-phase duration (lambda): (i) the technique used to monitor bacterial growth and (ii) the model fitted to estimate parameters. in this study, nine strains of listeria monocytogenes were monitored simultaneously by optical density (od) analysis and by viable count enumeration (vce) analysis. four usual growth models were fitted to our data, and estimates of growth parameters w ... | 2002 | 12450800 |
| molecular fingerprinting of dairy microbial ecosystems by use of temporal temperature and denaturing gradient gel electrophoresis. | numerous microorganisms, including bacteria, yeasts, and molds, constitute the complex ecosystem present in milk and fermented dairy products. our aim was to describe the bacterial ecosystem of various cheeses that differ by production technology and therefore by their bacterial content. for this purpose, we developed a rapid, semisystematic approach based on genetic profiling by temporal temperature gradient electrophoresis (ttge) for bacteria with low-g+c-content genomes and denaturing gradien ... | 2004 | 15345452 |
| pore size dependence on growth temperature is a common characteristic of the major outer membrane protein oprf in psychrotrophic and mesophilic pseudomonas species. | pseudomonas species adapt well to hostile environments, which are often subjected to rapid variations. in these bacteria, the outer membrane plays an important role in the sensing of environmental conditions such as temperature. in previous studies, it has been shown that in the psychrotrophic strain p. fluorescens mf0, the major porin oprf changes its channel size according to the growth conditions and could affect outer membrane permeability. studies of the channel-forming properties of oprfs ... | 2004 | 15528532 |
| pentose oxidation by pseudomonas fragi. | | 1961 | 13783864 |
| studies with a constitutive aldose dehydrogenase in pseudomonas fragi. | | 1963 | 13999575 |
| production of lipase by pseudomonas fragi in a synthetic medium. | alford, john a. (u.s. department of agriculture, beltsville, md.) and david a. pierce. production of lipase by pseudomonas fragi in a synthetic medium. j. bacteriol. 86:24-29. 1963.-the growth and production of lipase by pseudomonas fragi in 41 dehydrated media were determined. from a chemical analysis of the peptone medium which gave the maximal lipase production, numerous synthetic media were prepared. good cell growth, but no lipase production, was obtained on a buffered ammonium sulfate-gluc ... | 1963 | 14051818 |
| the metabolism of galactarate, d-glucarate and various pentoses by species of pseudomonas. | 1. when nad(+) was present, cell extracts of pseudomonas (a) grown with d-glucarate or galactarate converted 1mol. of either substrate into 1mol. each of 2-oxoglutarate and carbon dioxide; 70-80% of the gas originated from c-1 of the hexarate. 2. the enzyme system that liberated carbon dioxide from galactarate was inactive in air and was stabilized by galactarate or fe(2+) ions; the system that acted on d-glucarate was more stable and was stimulated by mg(2+) ions. 3. when nad(+) was not added, ... | 1965 | 14333567 |
| plasmid donor affects host range of promiscuous incp-1beta plasmid pb10 in an activated-sludge microbial community. | horizontal transfer of multiresistance plasmids in the environment contributes to the growing problem of drug-resistant pathogens. even though the plasmid host cell is the primary environment in which the plasmid functions, possible effects of the plasmid donor on the range of bacteria to which plasmids spread in microbial communities have not been investigated. in this study we show that the host range of a broad-host-range plasmid within an activated-sludge microbial community was influenced b ... | 2005 | 16151119 |
| effect of microorganism characteristics on leak size critical to predicting package sterility. | the effects of microorganism size and motility on the leak size critical to the sterility of a package, along with the imposed pressure required to initiate liquid flow for the critical leak size, were measured. pseudomonas fragi lacy-1052, bacillus atrophaeus atcc 49337, and enterobacter aerogenes atcc 29007 were employed to assess package sterility. one hundred twenty-six 7-mm-long microtubes with interior diameters of 5, 10, and 20 microm were used to simulate package defects. forty-two solid ... | 2003 | 14503733 |
| antimicrobial properties of human lysozyme transgenic mouse milk. | the antimicrobial properties of standard human lysozyme and the milk of transgenic mice expressing human lysozyme were investigated using bacterial strains important to the dairy industry. standard human lysozyme was found to be effective at significantly slowing the growth of the milk cold-spoilage organism pseudomonas fragi (p < 0.001), of a clinical isolate of the mastitis-causing organism staphylococcus aureus (p < 0.005), and a nonpathogenic strain of e. coli (p < 0.05). milk from transgeni ... | 1998 | 9708253 |
| effect of environmental and physiological conditions on the phase of adjustment of pseudomonas fragi. | the duration of the phase of adjustment of pseudomonas fragi was affected by the physiological age and growth temperature of the inoculum, as well as by the temperature at which the growth curve was determined. cultures in the exponential phase of growth gave shorter lags than stationary-phase and resting-phase inocula. inocula from the latter phase gave the longest lags. inocula grown at the temperature at which the growth curve was determined usually gave the shortest lags: the greater the dif ... | 1963 | 16349631 |
| acyl-coa synthetase catalyzes the synthesis of diadenosine hexaphosphate (ap6a). | the synthesis of diadenosine hexaphosphate (ap6a), a potent vasoconstrictor, is catalyzed by acyl-coa synthetase from pseudomonas fragi. in a first step amp is transferred from atp to tetrapolyphosphate (p4) originating adenosine pentaphosphate (p5a) which, subsequently, is the acceptor of another amp moiety from atp generating diadenosine hexaphosphate (ap6a). diadenosine pentaphosphate (ap5a) and diadenosine tetraphosphate (ap4a) were also synthesized in the course of the reaction. in view of ... | 1999 | 10385004 |
| effect of growth temperature on hydrolytic and esterifying activities from pseudomonas fragi crda 037 grown on whey | the production of hydrolytic and esterifying activities of pseudomonas fragi crda 037 grown on a whey-based medium was investigated at different temperatures over time. the optimal temperature was found to be critical and different for the production of both activities. the highest hydrolytic activity was detected with bacteria cultivated at between 24 degrees c (149.2 u/liter) and 27 degrees c (133.8 u/liter), while the highest production of ethyl valerate (esterifying activity) was observed by ... | 1999 | 10388711 |
| isolation and identification of antimicrobial furocoumarins from parsley. | photoactive furocoumarins extracted from four varieties of fresh and freeze-dried parsley leaves inhibited a dna repair-deficient escherichia coli in a photobiological assay. using media-modified assays, the human pathogens e. coli o157:h7 and listeria monocytogenes, the spoilage microorganism erwinia carotovora, and listeria innocua were also inhibited. pseudomonas fragi was not inhibited. minimum concentrations of forest green parsley powder in agar which showed inhibition ranged from 0.12% to ... | 1997 | 10465045 |
| differential protein expression by pseudomonas fragi submitted to various stresses. | we have analyzed the impact of various stressing conditions on the physiological and molecular responses of the main psychrotrophic spoilage bacterium of refrigerated meat and meat products, pseudomonas fragi. a survival study using conventional plating was first performed to select the stressing agents and parameters. some of these mimicked cleaning-disinfection processes but with less drastic conditions in order to keep alive enough bacterial cells for the protein expression characterization. ... | 1999 | 10493125 |
| transcriptional analysis of long-term adaptation of yersinia enterocolitica to low-temperature growth. | to analyze the transcriptional response of yersinia enterocolitica cells to prolonged growth at low temperature, a collection of luxcdabe transposon mutants was cultivated in parallel at optimal (30 degrees c) and suboptimal (10 degrees c) temperatures and screened for enhanced promoter activities during growth until entering stationary phase. among 5,700 y. enterocolitica mutants, 42 transcriptional units were identified with strongly enhanced or reduced promoter activity at 10 degrees c compar ... | 2006 | 16585756 |
| cloning and sequencing of the ompa gene of enterobacter sakazakii and development of an ompa-targeted pcr for rapid detection of enterobacter sakazakii in infant formula. | enterobacter sakazakii is an emerging, infant formula-borne pathogen that causes severe meningitis, meningoencephalitis, sepsis, and necrotizing enterocolitis in neonates and infants, with a high fatality rate. traditional detection methods take up to 7 days to identify e. sakazakii. the outer membrane protein a gene (ompa), along with its flanking sequences from e. sakazakii (atcc 51329), was cloned in the pgem-t easy vector and sequenced. comparison of the nucleotide and deduced amino acid seq ... | 2006 | 16597955 |
| inhibiting sulfate-reducing bacteria in biofilms on steel with antimicrobial peptides generated in situ. | in batch and continuous fermentations, the reduction in corrosion of sae 1018 mild steel and 304 stainless steel caused by inhibition of the reference sulfate-reducing bacterium (srb) desulfovibrio vulgaris by a protective, antimicrobial-producing bacillus brevis biofilm was investigated. the presence of d. vulgaris produced a thick black precipitate on mild steel and a higher corrosion rate in batch cultures than that seen in a mono-culture of non-antimicrobial-producing pseudomonas fragi k upo ... | 1999 | 10499267 |
| isolation and characterization of nickel uptake by nickel resistant bacterial isolate (nirbi). | bioremediation technology has gained importance because microbes could be the convenient source of bio-absorption/bioaccumulation of metals from effluent streams. | 2006 | 17044648 |
| effect of industrial and natural biocides on spoilage, pathogenic and technological strains grown in biofilm. | this study aimed at investigating bactericide solutions effective on spoilage and pathogenic bacteria while preserving technological bacteria. two compounds of essential oil (thymol and eugenol), one essential oil of satureja thymbra and two industrial biocides (pe 270-30, brillo) were tested on technological strains (staphylococcus equorum, staphylococcus succinus and lactobacillus sakei) grown in monoculture biofilm and on a mixed biofilm of pathogenic bacteria (staphylococcus aureus, listeria ... | 2007 | 17188206 |
| axenic aerobic biofilms inhibit corrosion of copper and aluminum. | the corrosion behavior of unalloyed copper and aluminum alloy 2024 in modified baar's medium has been studied with continuous reactors using electrochemical impedance spectroscopy. an axenic aerobic biofilm of either pseudomonas fragi k or bacillus brevis 18 was able to lessen corrosion as evidenced by a consistent 20-fold increase in the low-frequency impedance value of copper as well as by a consistent four- to seven-fold increase in the polarization resistance of aluminum 2024 after six days ... | 1999 | 10616712 |
| simultaneous detection of pseudomonas fragi, p. lundensis, and p. putida from meat by use of a multiplex pcr assay targeting the cara gene. | species-specific primers and a multiplex pcr assay were developed for the simultaneous identification and differentiation of pseudomonas fragi, p. lundensis, and p. putida based on the coamplification of different portions of the small subunit of the carbamoyl phosphate synthase gene (cara). the cara multiplex pcr was used to detect the presence of the three pseudomonas species from beef, chicken, and pork samples and proved to be effective in showing their evolution during the storage of meat. | 2007 | 17293505 |
| the growth and resistance to sodium hypochlorite of listeria monocytogenes in a steady-state multispecies biofilm. | a constant-depth film fermenter (cdff) was used to culture a steady-state multispecies biofilm consisting of one strain each of listeria monocytogenes, pseudomonas fragi and staphylococcus xylosus. these bacteria were initially grown together in a conventional chemostat to achieve a steady state before being inoculated into the cdff over an 18-h period. a dilute tryptone soya broth (tsb) medium was supplied to the cdff and the biofilm allowed to develop over a 28-d period. this mature biofilm wa ... | 2000 | 10747232 |
| [biochemistry and structural biology of microbial enzymes and their medical applications]. | microbial enzymes were studied from two medicinal viewpoints. first, we examined proline-specific peptidases from pathogenic microorganisms. we found several proline-specific peptidases in pathogenic bacteria. among them, prolyl tripeptidyl aminopeptidase from porphylomonas gingivals and prolyl aminopeptidase from serratia marcescens were crystallized. the complex structures of those enzymes and inhibitors were clarified in x-ray crystallography. aminopeptidase n, which has wide specificity for ... | 2007 | 17603262 |
| application of polynomial models to predict growth of mixed cultures of pseudomonas spp. and listeria in meat. | three models for one rapid and one slow growing strain of pseudomonas fragi and one slow growing strain of p. fluorescens were developed in a meat broth; they were designed to take account of variations in growth and to provide a growth response interval. these models, and another for listeria monocytogenes (lm14 model), were used to predict the growth of spoilage pseudomonas spp. and pathogenic listeria in meat products. the pseudomonas and listeria models provided satisfactory predictions conc ... | 2000 | 11028957 |
| oppa of listeria monocytogenes, an oligopeptide-binding protein required for bacterial growth at low temperature and involved in intracellular survival. | we identified a new oligopeptide permease operon in the pathogen listeria monocytogenes. this opp operon consists of five genes (oppa, oppb, oppc, oppd, and oppf) and displays the same genetic organization as those of several bacterial species. the first gene of this operon, oppa, encodes a 62-kda protein sharing 33% identity with oppa of bacillus subtilis and is expressed predominantly during exponential growth. the function of oppa was studied by constructing an oppa deletion mutant. the pheno ... | 2000 | 11083832 |
| attachment of bacteria to beef from steam-pasteurized carcasses. | the extent to which a bacterial cocktail containing equal numbers of pseudomonas fragi nctc 10689, listeria monocytogenes bl5/2, salmonella typhimurium lt2, and escherichia coli jm 109 attached to loin surface cuts (7 by 5 cm) derived from steam-pasteurized beef carcasses has been evaluated. the extent of attachment was categorized as loosely attached (removed by rinsing), firmly attached (released by stomaching), and irreversibly bound. no significant difference (p > 0.10) in the attachment of ... | 2001 | 11307885 |
| shewanella putrefaciens adhesion and biofilm formation on food processing surfaces. | laboratory model systems were developed for studying shewanella putrefaciens adhesion and biofilm formation under batch and flow conditions. s. putrefaciens plays a major role in food spoilage and may cause microbially induced corrosion on steel surfaces. s. putrefaciens bacteria suspended in buffer adhered readily to stainless steel surfaces. maximum numbers of adherent bacteria per square centimeter were reached in 8 h at 25 degrees c and reflected the cell density in suspension. numbers of ad ... | 2001 | 11319118 |
| altered protein expression of streptococcus oralis cultured at low ph revealed by two-dimensional gel electrophoresis. | streptococcus oralis is the predominant aciduric nonmutans streptococcus isolated from the human dentition, but the role of this organism in the initiation and progression of dental caries has yet to be established. to identify proteins that are differentially expressed by s. oralis growing under conditions of low ph, soluble cellular proteins extracted from bacteria grown in batch culture at ph 5.2 or 7.0 were analyzed by two-dimensional (2-d) gel electrophoresis. thirty-nine proteins had alter ... | 2001 | 11472910 |
| identification of a protein biomarker unique to the pandemic o3:k6 clone of vibrio parahaemolyticus. | the present method of characterizing vibrio parahaemolyticus strains involves serotyping or detection methods based on assessment of the presence or absence of genes thought to be markers of an organism's pathogenicity. it is unclear whether these assays detect all pathogenic v. parahaemolyticus strains since a clear correlation between the presence of a particular gene and the organism's pathogenicity has not yet been observed. we have described a proteomics-based method to distinguish individu ... | 2004 | 15071022 |
| immunoaffinity purification and characterization of mitochondrial membrane-bound d-3-hydroxybutyrate dehydrogenase from jaculus orientalis. | the interconversion of two important energy metabolites, 3-hydroxybutyrate and acetoacetate (the major ketone bodies), is catalyzed by d-3-hydroxybutyrate dehydrogenase (bdh1: ec 1.1.1.30), a nad+-dependent enzyme. the eukaryotic enzyme is bound to the mitochondrial inner membrane and harbors a unique lecithin-dependent activity. here, we report an advanced purification method of the mammalian bdh applied to the liver enzyme from jerboa (jaculus orientalis), a hibernating rodent adapted to extre ... | 2008 | 18826626 |
| pseudomonas fluorescens biofilms subjected to phage phiibb-pf7a. | pseudomonas fluorescens is an important food spoilage organism, usually found in the form of biofilms. bacterial biofilms are inherently resistant to a variety of antimicrobial agents, therefore alternative methods to biofilm control, such as bacteriophages (phages) have been suggested. phage behavior on biofilms is still poorly investigated and needs further understanding. here we describe the application of phage phiibb-pf7, a newly isolated phage, to control p. fluorescens biofilms. the biofi ... | 2008 | 18954451 |
| a modeling study by response surface methodology and artificial neural network on culture parameters optimization for thermostable lipase production from a newly isolated thermophilic geobacillus sp. strain arm. | thermostable bacterial lipases occupy a place of prominence among biocatalysts owing to their novel, multifold applications and resistance to high temperature and other operational conditions. the capability of lipases to catalyze a variety of novel reactions in both aqueous and nonaqueous media presents a fascinating field for research, creating interest to isolate novel lipase producers and optimize lipase production. the most important stages in a biological process are modeling and optimizat ... | 2008 | 19105837 |
| closed complex of the d-3-hydroxybutyrate dehydrogenase induced by an enantiomeric competitive inhibitor. | d-3-hydroxybutyrate dehydrogenase (hbdh) from pseudomonas fragi showed a strict stereospecificity to the d-enantiomer of 3-hydroxybutyrate (d-3-hb) as a substrate. the l-enantiomer acts as a competitive inhibitor, with a k(i) value comparable to the k(m) value for d-3-hb. we have determined the crystal structures of the ternary complex of hbdh-nad(+)-l-3-hb and the binary complex of hbdh-nad(+). the former structure showed a so-called closed-form conformation, which is considered an active form ... | 2009 | 19122202 |
| regulation of pyrimidine nucleotide formation in pseudomonas taetrolens atcc 4683. | the regulation of the de novo pyrimidine biosynthetic enzymes in the food spoilage agent pseudomonas taetrolens atcc 4683 was investigated. the de novo pyrimidine biosynthetic enzyme activities were determined in p. taetrolens atcc 4683 cells and in cells from an auxotroph deficient for orotidine 5'-monophosphate decarboxylase activity. pyrimidine supplementation to the culture medium affected the biosynthetic enzyme activities in atcc 4683 cells. transcriptional regulation of the biosynthetic p ... | 2004 | 15160604 |
| structural basis for channelling mechanism of a fatty acid beta-oxidation multienzyme complex. | the atomic view of the active site coupling termed channelling is a major subject in molecular biology. we have determined two distinct crystal structures of the bacterial multienzyme complex that catalyzes the last three sequential reactions in the fatty acid beta-oxidation cycle. the alpha2beta2 heterotetrameric structure shows the uneven ring architecture, where all the catalytic centers of 2-enoyl-coa hydratase (ech), l-3-hydroxyacyl-coa dehydrogenase (hacd) and 3-ketoacyl-coa thiolase (kact ... | 2004 | 15229654 |
| establishment of oxidative d-xylose metabolism in pseudomonas putida s12. | the oxidative d-xylose catabolic pathway of caulobacter crescentus, encoded by the xylxabcd operon, was expressed in the gram-negative bacterium pseudomonas putida s12. this engineered transformant strain was able to grow on d-xylose as a sole carbon source with a biomass yield of 53% (based on g [dry weight] g d-xylose(-1)) and a maximum growth rate of 0.21 h(-1). remarkably, most of the genes of the xylxabcd operon appeared to be dispensable for growth on d-xylose. only the xyld gene, encoding ... | 2009 | 19270113 |
| heterogeneity of heat-resistant proteases from milk pseudomonas species. | pseudomonas fragi, pseudomonas lundensis and members of the pseudomonas fluorescens group may spoil ultra high temperature (uht) treated milk and dairy products, due to the production of heat-stable proteases in the cold chain of raw milk. since the aprx gene codes for a heat-resistant protease in p. fluorescens, the presence of this gene has also been investigated in other members of the genus. for this purpose an aprx-screening pcr test has been developed. twenty-nine representatives of import ... | 2009 | 19481283 |
| cleavage and proteasome-mediated degradation of the basal transcription factor tfiia. | the transcription factor tfiia is encoded by two genes, tfiiaalphabeta and tfiiagamma. in higher eukaryotes, the tfiiaalphabeta is translated as a precursor and undergoes proteolytic cleavage; the regulation and biological implications of the cleavage have remained elusive. we determined by edman degradation that the tfiiabeta subunit starts at asp 278. we found that a cleavage recognition site (crs), a string of amino acids qvdg at positions -6 to -3 from asp 278, is essential for cleavage. mut ... | 2004 | 15257296 |
| cold shock of a hyperthermophilic archaeon: pyrococcus furiosus exhibits multiple responses to a suboptimal growth temperature with a key role for membrane-bound glycoproteins. | the hyperthermophilic archaeon, pyrococcus furiosus, was grown on maltose near its optimal growth temperature, 95 degrees c, and at the lower end of the temperature range for significant growth, 72 degrees c. in addition, cultures were shocked by rapidly dropping the temperature from 95 to 72 degrees c. this resulted in a 5-h lag phase, during which time little growth occurred. transcriptional analyses using whole-genome dna microarrays representing 2,065 open reading frames (orfs) in the p. fur ... | 2005 | 15601718 |
| fadd from pseudomonas putida ca-3 is a true long-chain fatty acyl coenzyme a synthetase that activates phenylalkanoic and alkanoic acids. | a fatty acyl coenzyme a synthetase (fadd) from pseudomonas putida ca-3 is capable of activating a wide range of phenylalkanoic and alkanoic acids. it exhibits the highest rates of reaction and catalytic efficiency with long-chain aromatic and aliphatic substrates. fadd exhibits higher k(cat) and k(m) values for aromatic substrates than for the aliphatic equivalents (e.g., 15-phenylpentadecanoic acid versus pentadecanoic acid). fadd is inhibited noncompetitively by both acrylic acid and 2-bromooc ... | 2009 | 19820085 |
| rna-binding domain of the key structural protein p7 for the rice dwarf virus particle assembly. | the rice dwarf virus (rdv) p7 structural protein is the key protein in the rdv particle assembly. the p7 protein was digested partially or completely by staphylococcus aureus v8 protease and/or pseudomonas fragi asp-n protease. the molecular mass and the n-terminal amino acid sequence of the polypeptide fragments of the p7 protein were determined by sds-page and the edman degradation method, respectively. then the polypeptides were located in the deduced amino acid sequence of the rdv p7 protein ... | 2005 | 15645082 |
| mutations in the "lid" region affect chain length specificity and thermostability of a pseudomonas fragi lipase. | the cold-adapted pseudomonas fragi lipase (pfl) displays highest activity on short-chain triglyceride substrates and is rapidly inactivated at moderate temperature. sequence and structure comparison with homologous lipases endowed with different substrate specificity and stability, pointed to three polar residues in the lid region, that were replaced with the amino acids conserved at equivalent positions in the reference lipases. substitutions at residues t137 and t138 modified the lipase chain- ... | 2005 | 15848176 |
| production of volatile compounds in cheese by pseudomonas fragi strains of dairy origin. | volatile compounds produced in cheese by five pseudomonas fragi strains isolated from 1-day-old raw milk cheeses were investigated. each strain was representative of a different biochemical group of isolates of identical phenotypic characteristics, according to identification with api 20 ne strips. the five strains were ascribed to the species p. fragi after 16s rrna sequencing because of their high degree of coincidence with p. fragi atcc 4973. in each of two experiments, carried out on differe ... | 2005 | 16013377 |
| posttranslational control of transcription factor fixk2, a key regulator for the bradyrhizobium japonicum-soybean symbiosis. | rhizobial fixk-like proteins play essential roles in activating genes for endosymbiotic life in legume root nodules, such as genes for micro-oxic respiration. in the facultative soybean symbiont, bradyrhizobium japonicum, the fixk(2) protein is the key player in a complex regulatory network. the fixk(2) gene itself is activated by the 2-component regulatory system fixlj in response to a moderate decrease of the oxygen tension, and the fixk(2) protein distributes and amplifies this response to th ... | 2009 | 19955406 |
| identification and characterization of di- and tripeptide transporter dtpt of listeria monocytogenes egd-e. | listeria monocytogenes is a gram-positive intracellular pathogen responsible for opportunistic infections in humans and animals. here we identified and characterized the dtpt gene (lmo0555) of l. monocytogenes egd-e, encoding the di- and tripeptide transporter, and assessed its role in growth under various environmental conditions as well as in the virulence of l. monocytogenes. uptake of the dipeptide pro-[14c]ala was mediated by the dtpt transporter and was abrogated in a deltadtpt isogenic de ... | 2005 | 16204487 |
| d-3-hydroxybutyrate dehydrogenase from pseudomonas fragi: molecular cloning of the enzyme gene and crystal structure of the enzyme. | the gene coding for d-3-hydroxybutyrate dehydrogenase (hbdh) was cloned from pseudomonas fragi. the nucleotide sequence contained a 780 bp open reading frame encoding a 260 amino acid residue protein. the recombinant enzyme was efficiently expressed in escherichia coli cells harboring phbdh11 and was purified to homogeneity as judged by sds-page. the enzyme showed a strict stereospecificity to the d-enantiomer (3r-configuration) of 3-hydroxybutyrate as a substrate. crystals of the ligand-free hb ... | 2006 | 16325199 |
| isolation and characterization of bacteria resistant to metallic copper surfaces. | metallic copper alloys have recently attracted attention as a new antimicrobial weapon for areas where surface hygiene is paramount. currently it is not understood on a molecular level how metallic copper kills microbes, but previous studies have demonstrated that a wide variety of bacteria, including escherichia coli, staphylococcus aureus, and clostridium difficile, are inactivated within minutes or a few hours of exposure. in this study, we show that bacteria isolated from copper alloy coins ... | 2010 | 20048058 |
| dehydrogenase activity of pseudomonas species. | single-strain cultures of pseudomonas fragi, p. fluorescens, p. putrefaciens, and strains of two marine species, pseudomonas type i and pseudomonas type ii, were found to be capable of reducing added acetaldehyde, proprionaldehyde, and butyraldehyde to the corresponding alcohols at 21 c. all species studied reduced propionaldehyde at 6 c. p. fragi, pseudomonas type i, and pseudomonas type ii reduced butanone at 6 and 21 c. p. fragi and pseudomonas type ii reduced acetone at 21 c. dehydrogenase a ... | 1967 | 16349730 |
| virulence and immunomodulatory roles of bacterial outer membrane vesicles. | outer membrane (om) vesicles are ubiquitously produced by gram-negative bacteria during all stages of bacterial growth. om vesicles are naturally secreted by both pathogenic and nonpathogenic bacteria. strong experimental evidence exists to categorize om vesicle production as a type of gram-negative bacterial virulence factor. a growing body of data demonstrates an association of active virulence factors and toxins with vesicles, suggesting that they play a role in pathogenesis. one of the most ... | 2010 | 20197500 |
| lipase from pseudomonas fragi. i. purification of the enzyme. | the experimental conditions required to isolate a lipase from pseudomonas fragi were determined. the organism was grown in a buffered tryptone medium for 4 to 5 days at 20 c. the lipase in the culture supernatant fluid was isolated by fractionation with ammonium sulfate at 60% saturation, followed by acetone precipitation at 30-60% concentration. further purification was made by using sephadex g-200 gel-filtration and diethylaminoethyl cellulose chromatography. electrophoretic analysis of the pu ... | 1969 | 16349851 |
| widespread head-to-head hydrocarbon biosynthesis in bacteria and role of olea. | previous studies identified the oleabcd genes involved in head-to-head olefinic hydrocarbon biosynthesis. the present study more fully defined the oleabcd protein families within the thiolase, alpha/beta-hydrolase, amp-dependent ligase/synthase, and short-chain dehydrogenase superfamilies, respectively. only 0.1 to 1% of each superfamily represents likely ole proteins. sequence analysis based on structural alignments and gene context was used to identify highly likely ole genes. selected microor ... | 2010 | 20418421 |
| lipase from pseudomonas fragi. ii. properties of the enzyme. | the optimal ph value of a lipase from pseudomonas fragi was between 7.5 and 8.9, and a high reaction rate was observed at 54 c. heating the enzyme solution at 63 c for 30 min inactivated only 27.6% of its activity; however, total inactivation was observed at 66 c after 1 hr and at 71 c after 10 min. the lipase was inhibited strongly by fe and fe ions, and to a lesser extent by co, cu, zn. no inhibition was observed with ca or naf. ethylenediaminetetraacetate was effective in removing the toxicit ... | 1969 | 16349852 |
| crystallization and preliminary x-ray characterization of d-3-hydroxybutyrate dehydrogenase from pseudomonas fragi. | a recombinant form of d-3-hydroxybutyrate dehydrogenase (ec 1.1.1.30) from pseudomonas fragi has been crystallized by the hanging-drop method using peg 3000 as a precipitating agent. the crystals belong to the orthorhombic group p2(1)2(1)2, with unit-cell parameters a = 64.3, b = 99.0, c = 110.2 a. the crystals are most likely to contain two tetrameric subunits in the asymmetric unit, with a vm value of 3.29 a3 da(-1). diffraction data were collected to a 2.0 a resolution using synchrotron radia ... | 2005 | 16508084 |
| distribution and genetic diversity of bacterial thiopurine methyltransferases in soils emitting dimethyl selenide. | dimethyl selenide (dmse) and dimethyl diselenide (dmdse) emissions by soil samples spiked with selenite or (methyl)selenocysteine, with or without a supplement of nutrient broth and glucose were measured. dmse was the main form of volatile se produced, and was observed for both se-substrates. dmdse was only emitted from soils spiked with (methyl)selenocysteine. two bacterial thiopurine methyltransferases (tpmts), tpmt-i and tpmt-e, have been reported to be involved in dmse and dmdse emissions [j ... | 2006 | 17007990 |
| human lysozyme expressed in the mammary gland of transgenic dairy goats can inhibit the growth of bacteria that cause mastitis and the cold-spoilage of milk. | the addition of human milk components with intrinsic antimicrobial activity to livestock milk by genetic engineering has the potential to benefit milk safety and production as well as the health of the lactating animal. as a model for the dairy cow, we generated transgenic goats that expressed human lysozyme in their milk at 68% of the levels found in human milk. milk from these transgenic animals had a bacteriostatic effect on both in vitro and in vivo growth of several microorganisms important ... | 2006 | 17199520 |
| survival of campylobacter jejuni under conditions of atmospheric oxygen tension with the support of pseudomonas spp. | campylobacter jejuni is a major food-borne pathogen. despite causing enteritis in humans, it is a well-adapted intestinal microorganism in animals, hardly ever generating disease symptoms. nevertheless, as a true microaerophilic microorganism it is still puzzling how campylobacter cells can survive on chicken meat, the main source of human infection. in this study, we demonstrate that c. jejuni is able to withstand conditions of atmospheric oxygen tension when cocultured with pseudomonas species ... | 2010 | 20639377 |
| quinolones: from antibiotics to autoinducers. | since quinine was first isolated, animals, plants and microorganisms producing a wide variety of quinolone compounds have been discovered, several of which possess medicinally interesting properties ranging from antiallergenic and anticancer to antimicrobial activities. over the years, these have served in the development of many synthetic drugs, including the successful fluoroquinolone antibiotics. pseudomonas aeruginosa and related bacteria produce a number of 2-alkyl-4(1h)-quinolones, some of ... | 2011 | 20738404 |
| quinolones: from antibiotics to autoinducers. | since quinine was first isolated, animals, plants and microorganisms producing a wide variety of quinolone compounds have been discovered, several of which possess medicinally interesting properties ranging from antiallergenic and anticancer to antimicrobial activities. over the years, these have served in the development of many synthetic drugs, including the successful fluoroquinolone antibiotics. pseudomonas aeruginosa and related bacteria produce a number of 2-alkyl-4(1h)-quinolones, some of ... | 2011 | 20738404 |
| quantification of cysteine oxidation in human estrogen receptor by mass spectrometry. | redox-dependent modifications of sulfhydryl groups within the two cys4 zinc fingers of the estrogen receptor dna-binding domain (er-dbd) result in structural damage and loss of er dna-binding function, which parallels the situation observed in many er-positive breast cancers. quantitation of the redox status of cysteinyl thiols within er-dbd employed cysteine-specific oxidants to induce varying degrees of oxidation in recombinant er, followed by differential alkylation with the stable isotopic l ... | 2007 | 17373775 |
| microarray-based characterization of the listeria monocytogenes cold regulon in log- and stationary-phase cells. | whole-genome microarray experiments were performed to define the listeria monocytogenes cold growth regulon and to identify genes differentially expressed during growth at 4 and 37 degrees c. microarray analysis using a stringent cutoff (adjusted p < 0.001; >/=2.0-fold change) revealed 105 and 170 genes that showed higher transcript levels in logarithmic- and stationary-phase cells, respectively, at 4 degrees c than in cells grown at 37 degrees c. a total of 74 and 102 genes showed lower transcr ... | 2007 | 17720827 |
| bacteroides: the good, the bad, and the nitty-gritty. | summary: bacteroides species are significant clinical pathogens and are found in most anaerobic infections, with an associated mortality of more than 19%. the bacteria maintain a complex and generally beneficial relationship with the host when retained in the gut, but when they escape this environment they can cause significant pathology, including bacteremia and abscess formation in multiple body sites. genomic and proteomic analyses have vastly added to our understanding of the manner in which ... | 2007 | 17934076 |
| multiple fadd acyl-coa synthetases contribute to differential fatty acid degradation and virulence in pseudomonas aeruginosa. | a close interconnection between nutrient metabolism and virulence factor expression contributes to the pathophysiology of pseudomonas aeruginosa as a successful pathogen. p. aeruginosa fatty acid (fa) degradation is complicated with multiple acyl-coa synthetase homologs (fadds) expressed in vivo in lung tissue during cystic fibrosis infections. the promoters of two genetically linked p. aeruginosa fadd genes (fadd1 and fadd2) were mapped and northern blot analysis indicated they could exist on t ... | 2010 | 21042406 |
| assessment of transformability of bacteria associated with tomato and potato plants. | transformation of plant-associated bacteria by plant dna has never been demonstrated in agricultural fields. in total 552 bacterial isolates from stems of ralstonia solanacearum-infected and healthy tomato plants and from stems and leaves of healthy potato plants were tested for natural genetic competence using plasmid psktg dna and homologous dna extracts. control strain acinetobacter baylyi adp1 was transformable with both dna extracts. no transformable isolates were observed after treatment w ... | 2007 | 17961482 |
| aerobic degradation of mercaptosuccinate by the gram-negative bacterium variovorax paradoxus strain b4. | the gram-negative bacterium variovorax paradoxus strain b4 was isolated from soil under mesophilic and aerobic conditions to elucidate the so far unknown catabolism of mercaptosuccinate (ms). during growth with ms this strain released significant amounts of sulfate into the medium. tn5::mob-induced mutagenesis was successfully employed and yielded nine independent mutants incapable of using ms as a carbon source. in six of these mutants, tn5::mob insertions were mapped in a putative gene encodin ... | 2010 | 21075928 |
| aerobic degradation of mercaptosuccinate by the gram-negative bacterium variovorax paradoxus strain b4. | the gram-negative bacterium variovorax paradoxus strain b4 was isolated from soil under mesophilic and aerobic conditions to elucidate the so far unknown catabolism of mercaptosuccinate (ms). during growth with ms this strain released significant amounts of sulfate into the medium. tn5::mob-induced mutagenesis was successfully employed and yielded nine independent mutants incapable of using ms as a carbon source. in six of these mutants, tn5::mob insertions were mapped in a putative gene encodin ... | 2010 | 21075928 |
| microbial synthesis of the energetic material precursor 1,2,4-butanetriol. | the lack of a route to precursor 1,2,4-butanetriol that is amenable to large-scale synthesis has impeded substitution of 1,2,4-butanetriol trinitrate for nitroglycerin. to identify an alternative to the current commercial synthesis of racemic d,l-1,2,4-butanetriol involving nabh4 reduction of esterified d,l-malic acid, microbial syntheses of d- and l-1,2,4-butanetriol have been established. these microbial syntheses rely on the creation of biosynthetic pathways that do not exist in nature. oxida ... | 2003 | 14570452 |
| molecular and phenotypic characterization of nonmotile gram-negative bacteria associated with spoilage of freshwater fish. | characterization of nonmotile bacteria associated with freshwater fish spoilage and that phenotypically resembled psychrobacter spp. | 2004 | 15012828 |
| seasonal influence on heat-resistant proteolytic capacity of pseudomonas lundensis and pseudomonas fragi, predominant milk spoilers isolated from belgian raw milk samples. | psychrotolerant bacteria and their heat-resistant proteases play a major role in the spoilage of uht-processed dairy products. summer and winter raw milk samples were screened for the presence of such bacteria. one hundred and three proteolytic psychrotolerant bacteria were isolated, characterized by api tests, rep-pcr fingerprint analysis and evaluated for heat-resistant protease production. twenty-nine strains (representing 79% of the complete collection) were further identified by 16s rrna ge ... | 2009 | 19196277 |
| mesophilic and psychrotrophic bacteria from meat and their spoilage potential in vitro and in beef. | mesophilic and psychrotrophic populations from refrigerated meat were identified in this study, and the spoilage potential of microbial isolates in packaged beef was evaluated by analyzing the release of volatile organic compounds (voc) by gas chromatography-mass spectrometry (gc/ms). fifty mesophilic and twenty-nine psychrotrophic isolates were analyzed by random amplified polymorphic dna-pcr, and representative strains were identified by 16s rrna gene sequencing. carnobacterium maltaromaticum ... | 2009 | 19201980 |
| dolichol phosphate mannose synthase1 mediates the biogenesis of isoprenyl-linked glycans and influences development, stress response, and ammonium hypersensitivity in arabidopsis. | the most abundant posttranslational modification in nature is the attachment of preassembled high-mannose-type glycans, which determines the fate and localization of the modified protein and modulates the biological functions of glycosylphosphatidylinositol-anchored and n-glycosylated proteins. in eukaryotes, all mannose residues attached to glycoproteins from the luminal side of the endoplasmic reticulum (er) derive from the polyprenyl monosaccharide carrier, dolichol p-mannose (dol-p-man), whi ... | 2011 | 21558543 |
| understanding structural features of microbial lipases--an overview. | the structural elucidations of microbial lipases have been of prime interest since the 1980s. knowledge of structural features plays an important role in designing and engineering lipases for specific purposes. significant structural data have been presented for few microbial lipases, while, there is still a structure-deficit, that is, most lipase structures are yet to be resolved. a search for 'lipase structure' in the rcsb protein data bank (http://www.rcsb.org/pdb/) returns only 93 hits (as o ... | 2008 | 19609386 |
| microbial metabolites and bacterial diversity in beef stored in different packaging conditions monitored by pyrosequencing, pcr-dgge, spme-gc/ms and 1hnmr. | beef chops were stored at 4°c in different conditions: air (a); modified atmosphere packaging (map); vacuum (v); bacteriocin-activated antimicrobial packaging (av). after 0 to 45 days of storage, analyses were performed to determine loads of spoilage microorganisms, microbial metabolites by spme-gc/ms and (1)hnmr, microbial diversity by pcr-dgge and pyrosequencing. the microbiological shelf-life of meat increased with increasing selectivity of storage conditions. the culture-independent analysis ... | 2011 | 21803905 |
| antarctic bacteria inhibit growth of food-borne microorganisms at low temperatures. | the aim of this study was to identify antarctic microorganisms with the ability to produce cold-active antimicrobial compounds with potential for use in chilled food preservation. colonies (4496) were isolated from 12 antarctic soil samples and tested against listeria innocua, pseudomonas fragi and brochothrix thermosphacta. thirteen bacteria were confirmed as being growth-inhibitor producers (detection rate 0.29%). when tested against a wider spectrum of eight target organisms, some of the isol ... | 2004 | 19712399 |
| evolution by leaps: gene duplication in bacteria. | sequence related families of genes and proteins are common in bacterial genomes. in escherichia coli they constitute over half of the genome. the presence of families and superfamilies of proteins suggest a history of gene duplication and divergence during evolution. genome encoded protein families, their size and functional composition, reflect metabolic potentials of the organisms they are found in. comparing protein families of different organisms give insight into functional differences and ... | 2009 | 19930658 |
| iron sources used by the nonpathogenic lactic acid bacterium lactobacillus sakei as revealed by electron energy loss spectroscopy and secondary-ion mass spectrometry. | lactobacillus sakei is a lactic acid bacterium naturally found on meat. although it is generally acknowledged that lactic acid bacteria are rare species in the microbial world which do not have iron requirements, the genome sequence of l. sakei 23k has revealed quite complete genetic equipment dedicated to transport and use of this metal. here, we aimed to investigate which iron sources could be used by this species as well as their role in the bacterium's physiology. therefore, we developed a m ... | 2010 | 19933352 |
| iron sources used by the nonpathogenic lactic acid bacterium lactobacillus sakei as revealed by electron energy loss spectroscopy and secondary-ion mass spectrometry. | lactobacillus sakei is a lactic acid bacterium naturally found on meat. although it is generally acknowledged that lactic acid bacteria are rare species in the microbial world which do not have iron requirements, the genome sequence of l. sakei 23k has revealed quite complete genetic equipment dedicated to transport and use of this metal. here, we aimed to investigate which iron sources could be used by this species as well as their role in the bacterium's physiology. therefore, we developed a m ... | 2010 | 19933352 |