| the reaction of pseudomonas aeruginosa cytochrome c-551 oxidase with oxygen. | the reaction of ascorbate-reduced pseudomonas cytochrome oxidase with oxygen was studied by using stopped-flow techniques at ph 7.0 and 25 degrees c. the observed time courses were complex, the reaction consisting of three phases. of these, only the fastest process, with a second-order rate constant of 3.3 x 10(4) m-1.s-1, was dependent on oxygen concentration. the two slower processes were first-order reactions with rates of 1.0 +/- 0.4s-1 and 0.1 +/- 0.03s-1. a kinetic titration experiment rev ... | 1978 | 210764 |
| evolutionary change of the heme c electronic structure: ferricytochrome c-551 from pseudomonas aeruginosa and horse heart ferricytochrome c. | | 1978 | 213063 |
| influence of suspending media upon the susceptibility of pseudomonas aeruginosa nctc 6750 and its spheroplasts to polymyxin b. | the lytic and bactericidal actions of polymyxin b on whole cells and spheroplasts of pseudomonas aeruginosa varied markedly with the suspending media, and there was little correlation between them. relative rates of lysis of these preparations and also of bacillus megaterium protoplasts suggested that polymyxin causes progressive damage to the cytoplasmic membrane, such that membrane permeability towards various ions increased as follows: k(+) > na(+) > no(3) (-) > cl(-), ca(2+), h(2)po(4) (-)/h ... | 1979 | 218501 |
| mössbauer studies of cytochrome c-551. intrinsic heterogeneity related to g-strain. | the mössbauer spectra of oxidized and reduced cytochrome c-551 from pseudomonas aeruginosa are analyzed. excess broadening is observed in the 4.2 k spectra of oxidized c-551 which is consistent with a gaussian distribution of the crystal field parameters delta and r as inferred from the g-strain model of epr line shapes. | 1979 | 218635 |
| chemical alterations in cell envelopes of polymyxin-resistant pseudomonas aeruginosa isolates. | cell envelopes from pseudomonas aeruginosa strains resistant to polymyxin were compared with cell envelopes from polymyxin-sensitive strains as to their content of total protein, carbohydrate, and 2-keto-3-deoxyoctonate and as to their protein composition as determined by slab polyacrylamide gel electrophoresis. the cell envelopes of the polymyxin-resistant strains had reduced amounts of lipopolysaccharide, as indicated a reduction in both carbohydrate and 2-keto-3-deoxyoctonate concentrations, ... | 1979 | 222726 |
| [pseudomonas aeruginosa melanin]. | the epr, ir, uv and visible absorption spectra of a melanin-like pigment from p. aeruginosa were studied. by the whole complex of the spectral characteristics the pigment may be classified as belonging to the melanin group. the study of the antibiotic properties of the pigment showed that it did not inhibit the growth of p. aeruginosa and e. coli. still, it possessed some antistaphylococcal activity. | 1979 | 223498 |
| topical antibiotic therapy of pseudomonas aeruginosa keratitis. | the in vivo antibacterial effectiveness in the rabbit cornea of several commercially available ophthalmic antibiotic preparations was determined against a single strain of pseudomonas aeruginosa isolated from a human corneal ulcer. each antibiotic was instilled topically at hourly intervals, and the number of residual viable organisms in the cornea subsequently was ascertained. in vivo measurements correlated well with in vitro data and with generally held clinical impressions. three antibiotics ... | 1979 | 224848 |
| allosteric cooperative interactions among redox sites of pseudomonas cytochrome oxidase. | anaerobic reductive spectrophotometric titrations of pseudomonas aeruginosa cytochrome oxidase were performed. both types of hemes (c and d) of the dimeric enzyme were monitored. the reduction process was found to involve cooperative allosteric and spectroscopic interactions between the two subunits. the model fitting the data best involves the following features. (1) the redox potential of heme c is about 60 mv higher than that of heme d. (2) in the electron uptake, a positive cooperativity of ... | 1979 | 224908 |
| isolation of oxidase-negative pseudomonas aeruginosa from sputum culture. | two isolates of pseudomonas aeruginosa lacking characteristic indophenol oxidase were recovered from a sputum specimen. a discussion of the characteristic biochemical tests and antibiograms along with a possible explanation for this phenomenon is presented. | 1979 | 225349 |
| biosynthesis of proline in pseudomonas aeruginosa. partial purification and characterization of gamma-glutamyl kinase. | a gamma-glutamyl kinase (atp-l-glutamate 5-phosphotransferase) was purified about 85-fold from crude extracts of pseudomonas aeruginosa strain pao 1 by (nh4)2so4 precipitation, molecular-sieving by sephadex g-150 and deae-cellulose chromatography. the molecular weight of this enzyme was 84,000. the preparation catalysed formation of gamma-glutamyl hydroxamate from l-glutamate, atp and mg2+ or mn2+ with concomitant hydrolysis of atp to adp + pi. l-proline inhibited the gamma-glutamyl kinase activ ... | 1979 | 226082 |
| circular dichroism studies on cytochrome c peroxidase and cytochrome c-551 of pseudomonas aeruginosa. | circular dichroism (cd) spectra of ferric, ferrous and ferrous-carbonyl forms of pseudomonas cytochrome c peroxidase have been recorded in the wave length range 200 to 650 nm. cd spectra in the soret region show that in the oxidized enzyme the two hemes are degenerate, whereas in the reduced form the hemes are perturbed differently and one of the hemes appears to be non-degenerate. changes in optical activity upon formation of the carbonylderivative suggest a spin-state conversion and indicate t ... | 1979 | 226160 |
| electron transfer kinetics between rhus vernicifera stellacyanin and cytochrome c (horse heart cytochrome c and pseudomonas cytochrome c551). | the electron transfer reactions between rhus vernicifera stellacyanin and either horse heart cytochrome c or pseudomonas aeruginosa cytochrome c551 were investigated by rapid reaction techniques. the time course of electron transfer is monophasic under all conditions, and thus consistent with a simple formulation of the reaction. both stopped-flow and temperature-jump experiments yield equilibrium constants in reasonable agreement with values calculated from the redox potentials. the differences ... | 1979 | 228006 |
| [clinical studies on pivmecillinam in urinary tract infections (author's transl)]. | pivmecillinam (pmpc), semisynthetic penicillin for oral use, was studied clinically and following results were obtained. 1) twenty-eight patients with acute simple cystitis treated with the drug 200 mg/day for 4 days, clinical results were excellent in 23 cases and moderate in 5 cases. six patients with chronic simple cystitis treated with the drug 400 mg/day for 7 days, clinical results were excellent in 4 cases, moderate in 1 case and poor in 1 case. eight patients with complicated urinary tra ... | 1979 | 228097 |
| lethal effect of complement and lysozyme on polymyxin-treated, serum-resistant gram-negative bacilli. | when genetically serum-resistant escherichia coli, klebsiella pneumoniae, and citrobacter freundii, but not pseudomonas aeruginosa or proteus mirabilis, were exposed to polymyxin b, they became susceptible to the bactericidal action of normal human and rabbit sera. in constrast, beta-lactam and aminoglycoside antibiotics did not render any serum-resistant bacteria serum-sensitive. synergy between polymyxin b and the serum bactericidal system could be demonstrated by the addition of polymyxin b t ... | 1979 | 229175 |
| crystal data, molecular dimensions and molecular symmetry in cytochrome oxidase from pseudomonas aeruginosa. | | 1979 | 231108 |
| effect of divalent cations on the inhibition of alkylsulfatase induction and the generation of atp in pseudomonas aeruginosa after exposure to exogenous uridine 5'-triphosphate. | in the absence of added mg2+, alkylsulfatase induction in resting cells of pseudomonas aeruginosa was inhibited 17% by exogenous 0.05 mm utp. under these conditions, the cells converted utp to atp and rapid degradation of these nucleotides did not occur. in the presence of 0.73 mm mg2+, 0.05 mm utp repressed the synthesis of the enzyme by 71%. under these conditions, the cells rapidly degraded both atp derived from utp as well as residual utp. in the presence of mg2+ and 0.1 mm utp, full repress ... | 1979 | 232003 |
| synergism between chlorhexidine and polymyxins against pseudomonas aeruginosa. | | 1979 | 232100 |
| the influence of calcium or manganese on the resistance to edta, polymyxin b or cold shock, and the composition of pseudomonas aeruginosa grown in glucose- or magnesium-depleted batch cultures. | | 1979 | 232101 |
| chromosomal mobilization from a reca mutant of pseudomonas putida. | a methyl methane sulfonate sensitive mutant of p. putida strain ppg1 is also extremely sensitive to uv-rays, compared to parent wild type cells. this mutant behaves typically as recombination less (reca) mutants of escherichia coli and pseudomonas aeruginosa, since as a recipient, it exhibits extremely low frequency of recombination following conjugational, transductional, and transformational gene transfer. sex factor plasmids such as k-xyl or tol can mobilize chromosomal genes equally well bot ... | 1979 | 232230 |
| [nonspecific prophylaxis and therapy of pseudomonas aeruginosa wound-infections with paramunization using a mouse-model (author's transl)]. | the effectiveness of paramunization as a antigen nonspecific method to activate mechanisms against wound-infections due to pseudomonas aeruginosa was studied using a model of direct infection of mice with a "mice-pathogenic" ps. aeruginosa strain on artificially set wounds. active paramunization by means of a biological inducer "pind-avi" (m-hp 438) significantly reduced the mortality rate between treated and placebo animals. the best results were obtained by parenteral prophylactic application. ... | 1979 | 232340 |
| glycine metabolism by pseudomonas aeruginosa: hydrogen cyanide biosynthesis. | hydrogen cyanide (hcn) production by pseudomonas aeruginosa in a synthetic medium is stimulated by the presence of glycine. methionine enhances this stimulation but will not substitute for glycine as a stimulator of cyanogenesis. threonine and phenylalanine are effective substitutes for glycine in the stimulation of hcn production. glycine, threonine, and serine are good radioisotope precursors of hcn, but methionine and phenylalanine are not. cell extracts of p. aeruginosa convert [14c]threonin ... | 1977 | 233722 |
| gram-negative bacillary endocarditis. interpretation of the serum bactericial test. | although the serum bactericidal test is commonly used in the management of infective endocarditis, little has been written about its validity or limitations. we report three cases of gram-negative bacillary endocarditis (pseudomonas aeruginosa, vibrio fetus and serratia marcescens) encountered in 1 year at a veterans administration hospital. serum bactericidal titers were considered necessary to identify inadequate antibiotic regimens or to avoid unnecessary drug toxicity. the limitations of the ... | 1975 | 234679 |
| purification and properties of a constitutive beta-lactamase from pseudomonas aeruginosa strain dalgleish. | 1. the beta-lactamase (penicillin amido-beta-lactamhydrolase ec 3.5.2.6) appeared to be periplasmic rather than truly intracellular, since it was released by freeze-thawing without gross morphological changes in the cell. 2. the partially purified enzyme had pi between 5.0 and 5.5, mol. wt 32 000 and a broad ph vs activity profile with a maximum at ph 8. 3. the cephalosporins tested were hydrolysed less rapidly than most of the penicillins, and the km values for penicillins were lower than for c ... | 1975 | 235307 |
| homogeneity and variability in the structure of azurin molecules studied by fluorescence decay and circular polarization. | the fluorescence decay of apoazurin derived from pseudomonas aeruginosa is monoexponential. by this criterion the population of molecules of apoazurin is homogeneous. the emission anisotropy factor and the absorption anisotropy factor at the red edge of the absorption band assume similar values, showing that the tryptophan residue in apoazurin has the same asymmetric environment both in the ground and excited states. this finding suggests tight packing of the protein at the tryptophan environmen ... | 1975 | 235970 |
| problems and progress towards vaccination against bacterial infections of the respiratory tract. | a large number of bacterial species are causative agents of respiratory tract disease. the discussion will center on three infections selected because they represent different problems in control on the basis of epidemiologic and immunochemical factors. group a hemolytic streptococci are common upper respiratory tract pathogens which may initiate severe non-suppurative sequelae such as rheumatic fever and glomerulonephritis. recent progress concerning m protein vaccines will be reviewed. pneumoc ... | 1975 | 236208 |
| nationwide epidemic of septicemia caused by contaminated infusion products. iv. growth of microbial pathogens in fluids for intravenous infusions. | septicemia caused by contaminated infusion fluid is a newly appreciated hazard of intravenous infusion therapy. microorganisms of the tribe klebsielleae (klebsiella, enterobacter, and serratia) have predominated in these infections. members of this tribe found to possess a selecive ability over common non-klebsielleae microbial pathogens to proliferate rapidly in commerical parential fluids contaning clucose at room temperautre. fifty-one klebsielleae strains, washed twice before inoculation of ... | 1975 | 236343 |
| aldehyde dehydrogenase from pseudomonas aeruginosa. | | 1975 | 236458 |
| the isolation and enumeration of pseudomonas aeruginosa from oily cream. | | 1975 | 237079 |
| inhibition of pathogenic enteric bacteria by hyperbaric oxygen: enhanced antibacterial activity in the absence of carbon dioxide. | the antibacterial effects of 24-h exposures to high-pressure oxygen in relation to environmental co(2) were studied at 3 atm absolute (ata) and at 1 ata. eight gram-negative, aerobic and facultatively aerobic, pathogenic enteric bacteria (salmonella typhosa, salmonella paratyphi, salmonella schottmuelleri, shigella dysenteriae, shigella flexneri, proteus vulgaris, pseudomonas aeruginosa, and escherichia coli) were exposed as shallow-broth cultures and agar surface cultures. although broths suppl ... | 1975 | 238466 |
| purification and partial characterization of a collagenolytic enzyme from pseudomonas aeruginosa. | a proteinase from pseudomonas aeruginosa exhibiting collagenolytic activity was purified 1575-fold with a recovery of 24% by use of chemical and chromatographic technics. the enzyme preparation appeared to be homogeneous when subjected to chromatographic, electrophoretic and ultracentrifugational analyses. a standard state sedimentation coefficient of 2.10 s was calculated and further analyses indicated that the enzyme had a molecular weight of 17 500 and dimerizes under certain conditions to yi ... | 1975 | 238605 |
| dual role for n-2-acetylornithine 5-aminotransferase from pseudomonas aeruginosa in arginine biosynthesis and arginine catabolism. | in pseudomonas aeruginosa n-2-acetylornithine 5-aminotransferase (acoat), the fourth enzyme of arginine biosynthesis is induced about 15-fold by cultivating the organism on a medium with l-arginine as the sole carbon and nitrogen source. synthesis of the enzyme is subject to catabolite repression and nitrogen source. synthesis of the enzyme is subject to catabolite repression by a variety of carbon sources. acoat from strain pao 1 was purified over 40-fold to electrophoretic homogeneity. a molec ... | 1975 | 238949 |
| [clinical effects of lividomycin on respiratory tract infections, primarily wet cases of bronchiectasis (author's transl)]. | patients with various respiratory infections, primarily wet cases of bronchiectasis, were treated with lividomycin (lvdm) at a dose of 1 g/day for about a week. the results obtained are as follows: 1. of the 15 patients with refractory bronchiectasis who had previously been treated with various other antibiotics, 11 patients (73.3%) responded to lvdm. although there was no patient who responded to lvdm excellently, good cures were achieved in 5 pateints and fair cures in 6 patients. 2. in bronc ... | 1975 | 239263 |
| [comparison of the antibacterial activity of amikacin (bb-k8) with other aminoglycosides against pathogens recently isolated from clinical materials (author's transl) ]. | we determined the antibacterial activity of amikacin against 1,277 strains of pathogenic bacteria isolated from clinical materials during 1974, including beta hemolytic streptococci, pneumococci, enterococci, staphylococcus aureus, staph. epidermidis, escherichia coli, klebsiella, enterobacter, citrobacter, serratia, proteus morganii and pseudomonas aeruginosa, and compared the minimum inhibitory concentration (mic) of this drug with gentamicin, dibekacin, tobramycin and kanamycin. 1)antibacteri ... | 1975 | 240047 |
| circular dichroism of holo- and apoprotocatechuate 3,4-dioxygenase from pseudomonas aeruginosa. | circular dichroism studies have been carried out on both apo- and holoprotocatechuate 3,4-dioxygenase from pseudomonas aeruginosa, in the absence and presence of competitive inhibitors, protocatechualdehyde and 4-nitrocatechol. the apo- and holoenzyme showed identical spectra in the ultraviolet region between 200 and 250 nm (peptide back bone region), but the low intensity negative bands at 330 and 480 nm of the holoenzyme were completely absent in the apoenzyme. on the side chain region, the po ... | 1975 | 240396 |
| n-acetylglutamate 5-phosphotransferase of pseudomonas aeruginosa. catalytic and regulatory properties. | some kinetic properties of n-acetylglutamate 5-phosphotransferase (atp: n-acetyl-l-glutamate 5-phosphotransferase ec 2.7.2.8) purified approx. 2000-fold from pseudomonas aeruginosa have been studied. the enzyme required mg2+ for activity. mn2+, zn2+, co2+, and ca2+, in this order, could replace mg2+ partially. the substrate specificity was narrow: n-carbamoyl-l-glutamate and n-formyl-l-glutamate were phosphorylated, but at a lower rate than n-acetyl-l-glutamate; n-propionyl-l-glutamate was almos ... | 1975 | 240684 |
| survival of anaerobic and aerobic bacteria on cotton swabs in three transport systems. | the capacity of aerobic and anaerobic bacteria to survive on cotton swabs placed into a dry gassed-out co2-filled tube (dgt), dry sterile aerobic tube (dat), and a tube containing a modified stuarts' transport medium (mst), was assessed. pseudomonas aeruginosa increased in numbers by 2 and 3 logs when stored in mst and dat, respectively. the viability of p. aeruginosa, although retarded when compared to msa and dat, was not adversely affected by the co2 environment in the dgt. the mst maintaine ... | 1975 | 240870 |
| determination of the isotope effect of the enzymatic oxidation of (r)carnitine by displacement of the equilibrium via mass action. | an isotope effect of the dehydrogenation of (r) carnitine [(r) 3-hydroxy-4-trimethylaminobutyric acid hydrochloride] catalyzed by (r) carnitine dehydrogenase [(r) carnitine: nad oxidoreductase e.c. 1.1.1.108] from pseudomonas aeruginosa was measured at different temperatures. it was found that k1h/k3h does not vary greatly with changes of temperature. the value of 3 for k1h/k3h measured at small initial conversions strongly indicated that the rate limiting step of the oxidation of (r) carnitine ... | 1975 | 241170 |
| secondary ocular bacterial infection in hypovitaminosis a xerophthalmia. | extraocular bacterial culture was performed in 100 patients with hypovitaminosis a xerophthalmia. there was corneal ulceration in 29 cases and corneal perforation in 22 cases. eighty-six percent of the patients harbored frankly pathogenic bacteria (46 patients) and potentially pathogenic bacteria (40 patients). all but two patients with either cornal ulceration or perforation harbored potentially pathogenic or frankly pathogenic bacteria. pseudomonas aeruginosa, diplococcus pneumoniae, and morax ... | 1975 | 241260 |
| antibacterial activity of cationic proteins from human granulocytes. | human granulocytes contain several cationic proteins with a molecular weight of approximately 25,000, almost identical amino acid composition, and complete immunologic identity. these proteins possess a chymotrypsin-like protease activity at a neutral ph. the antibacterial activity of the cationic proteins has been studied. bactericidal activities are found against both gram-positive (streptococcus faecalis and staphylococcus aureus) and gram-negative (escherichia coli and pseudomonas aeruginosa ... | 1975 | 241758 |
| infectious complications of burns casualties during the yom-kippur war. | during the october 1973 military conflict, 41 burned patients hospitalized at the sheba medical center were closely followed up. all the patients who had burns occupying 20% or more of the body surface developed wound infections. pseudomonas aeruginosa was the major pathogen isolated. most infections occurred during the second week following the injury. serial cultures revealed that only the isolation of proteus species and p. aeruginosa from wounds could predict an infection caused by those mic ... | 1977 | 244453 |
| a comparative study of ticarcillin plus tobramycin versus carbenicillin plus gentamicin for the treatment of serious infections due to gram-negative bacilli. | the combination of ticarcillin plus tobramycin (tt) or carbenicillin plus gentamicin (cg) was used to treat 82 patients with severe systemic gram-negative infection in a prospective, randomized study. pseudomonas aeruginosa was the primary pathogen in 7 (93 per cent) of these patients. patients treated with tt responded more frequently (92 per cent or 37 of 40) than patients treated with cg (71 per cent or 30 of 42) (p is less than 0.05). this difference was primarily due to a greater response t ... | 1978 | 247895 |
| antibacterial activity of apalcillin (pc-904) against gram-negative bacilli, especially ampicillin-, carbenicillin-, and gentamicin-resistant clinical isolates. | apalcillin (pc-904) is active against carbenicillin- and ampicillin-resistant strains of gram-negative bacilli. among pseudomonas aeruginosa strains highly resistant to carbenicillin (>/=3,200 mug/ml), half of them were susceptible to pc-904 at a concentration of 50 to 1,600 mug/ml. the minimal inhibitory concentration of pc-904 against p. aeruginosa strains resistant to carbenicillin (400 to 1,600 mug/ml) ranged from 3.1 to 25 mug/ml. ampicillin- and carbenicillin-resistant enterobacteriaceae s ... | 1978 | 248269 |
| comparative in vivo activities of cefsulodin, sulbenicillin, and gentamicin against pseudomonas aeruginosa. | the in vivo activities of cefsulodin, sulbenicillin, and gentamicin were compared in mice infected intraperitoneally with pseudomonas aeruginosa. in mice infected with sulbenicillin- and gentamicin-susceptible strains, cefsulodin was about 12 to 60 times more active than sulbenicillin and had an activity similar to gentamicin. in mice, the activity of cefsulodin was independent whether the strains were resistant to gentamicin or not, but it was considerably affected by resistance of the strains ... | 1978 | 249623 |
| carbenicillin for treatment of bacteroides fragilis infections: why not penicillin g? | carbenicillin has been advocated for treatment of infections caused by bacteroides fragilis and other anaerobic bacteria. wide-scale use of the drug in this setting could result in a substantial increase in carbenicillin-resistant pseudomonas aeruginosa, an effect that would have serious implications. thirty-four strains of b. fragilis, one-half from bacteremic infections, were tested in vitro, and penicillin g was found to be twice as active as carbenicillin on an equal weight basis; 94% of the ... | 1978 | 253006 |
| hr 756, a highly active cephalosporin: comparison with cefazolin and carbenicillin. | hr 756, a new parenteral cephalosporin, was compared with cefazolin and carbenicillin for activity against a total of 264 strains of pseudomonas aeruginosa, escherichia coli, klebsiella spp., proteus mirabilis, proteus spp. (indole positive), enterobacter spp., salmonella typhi, serratia marcescens, providencia stuartii, and staphylococcus aureus. in every comparison, except that with the last organism, hr 756 was clearly more active than cefazolin and carbenicillin. all three compounds had simi ... | 1978 | 253572 |
| comparative evaluation of piperacillin in vitro. | the minimal inhibitory concentrations of piperacillin and seven other betalactam antibiotics were determined against 407 bacterial isolates. piperacillin was found to be more active than ampicillin against susceptible gram-negative bacilli and more active than either carbenicillin or ticarcillin against pseudomonas aeruginosa and streptococci. although piperacillin was active against klebsiella pneumoniae, this activity was less than that of the cephalosporins. piperacillin was not active agains ... | 1978 | 253573 |
| continuous infusion tobramycin combined with carbenicillin for infections in cancer patients. | the cure rate of infections in cancer patients is adversely affected by neutropenia (less than 1,000/mm3). in particular, patients with severe neutropenia (less than 100/mm3) have shown a poor response to antibiotics. to overcome the adverse effects of neutropenia, tobramycin was given by continuous infusion and combined with intermittent carbenicillin. tobramycin was given to a total daily dose of 300 mg/m2 and carbenicillin was given at a dose of 5 gm every four hours. there were 125 infectiou ... | 1979 | 256433 |
| comparative activities of ampicillin, epicillin and amoxycillin in vitro and in vivo. | the antibacterial activities of three aminopenicillins ampicillin, epicillin and amoxycillin were compared in vitro and in vivo. the minimum inhibitory concentrations (mic) of the three penicillins were very similar and the compounds were active against non-beta-lactamase-producing strains of escherichia coli, salmonella and shigella species, proteus mirabilis, haemophilus influenzae and neisseria gonorrhoeae. streptococci including streptococcus faecalis, and non-beta-lactamase-producing staphy ... | 1979 | 256524 |
| experimental pseudomonas aeruginosa pneumonia. | | 1979 | 262511 |
| gram-negative sepsis in thermally injured sheep. | burn wound sepsis was studied for four days in awake, unanesthetized sheep. each of the animals was given a 40% third-degree burn, and the wound was infected with pseudomonas aeruginosa. according to their cardiopulmonary response, the animals were divided into three groups: hyperdynamic, normodynamic, and hypodynamic. the hyperdynamic group had an increased cardiac index and a decreased total peripheral resistance index. the hypodynamic group was characterized by the following: decreased cardia ... | 1979 | 262801 |
| specific gonadotropin binding to pseudomonas maltophilia. | binding of 125i-labeled human chorionic gonadotropin to pseudomonas maltophilia is dependent on time, temperature, and ph and the binding to this procaryotic species is hormone-specific and saturable. the equilibrium dissociation constant is 2.3 x 10(-9) m. there are no cooperative interactions between binding sites (hill coefficient, 1.05). the number of sites is estimaated as 240 fmol/100 mug of protein. nacl and kcl, at concentrations from 1 to 10 mm, have no effect on binding. divalent catio ... | 1977 | 265583 |
| prevalence and survival of microbial contaminants in heated nebulizers. | over a 2-year period, 600 cultures of fluid in heated nebulizers in use by patients in the peter bent brigham hospital were performed. the most commonly isolated microorganisms were pseudomonas and alcaligenes species. pseudomonas aeruginosa was never isolated. three types of heated nebulizers were in use, and their contamination rate was significantly different (45 percent, 21 percent, and 8 percent, respectively; p less than 0.001). in the course of the study, the overall contamination rate de ... | 1978 | 273384 |
| autoclaving of lubricated dental instruments. | test organisms forced mechanically into lubricated, rotating dental instruments (handpieces) were all killed during autoclaving at 134 degrees c for 8 min, even when protected by serum and oil. the test organisms were: escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, candida albicans, and spores of bacillus stearothermophilus. also when testing the sterility of autoclaved simulated instrument surfaces (brass cylinders and pieces of a cotton fabric) which had been inoculated with ... | 1978 | 274800 |
| transposition of plasmid dna segments specifying hydrocarbon degradation and their expression in various microorganisms. | the conjugative tol plasmid (75 mdal), specifying biodegradation of xylenes, toluene, and trimethylbenzene derivatives, undergoes dissociation in pseudomonas aeruginosa pao to a nonconjugative tol(*) plasmid (28 mdal) and a transfer plasmid termed toldelta (48 mdal). the tol(*) plasmid is rendered transmissible through introduction of a number of conjugative plasmids such as factor k, cam, and toldelta but not by the fp2 derivative pr0271. transfer of tol(*) via factor k or toldelta is mediated ... | 1978 | 277912 |
| subnanosecond motions of tryptophan residues in proteins. | the dynamics of protein molecules in the subnanosecond and nanosecond time range were investigated by time-resolved fluorescence polarization spectroscopy. synchrotron radiation from a storage ring was used as a pulsed light source to excite the single tryptophan residue in a series of proteins. the full width at half maximum of the detected light pulse was 0.65 nsec, making it feasible to measure emission anisotropy kinetics in the subnanosecond time range and thereby to resolve internal rotati ... | 1979 | 284374 |
| ultraviolet light induction of diphtheria toxin-resistant mutants of normal and xeroderma pigmentosum human fibroblasts. | the uv induction of diphtheria toxin-resistant (dtr) mutants in normal and xeroderma pigmentosum human fibroblasts has been quantitatively characterized. a concentration of diphtheria toxin at which dtr cells are cross-resistant to pseudomonas aeruginosa exotoxin a was determined and used in the selection of resistant mutants. recovery of mutants was not influenced by the presence of wild-type cell densities of 1-8 x 10(5) per 9-cm plate, indicating no metabolic cooperation exists, in contrast t ... | 1979 | 291058 |
| case report: pseudomonas aeruginosa infection of the lips. | | 1979 | 298751 |
| [depressor effect and primary resistance of the cornea against pseudomonas pyocyanea (author's transl)]. | as ps. pyocyanea seems to replace previously dominant germs as the main cause of corneal infections, the influence of chloromycetin and actinomycin d on the primary resistance of the cornea and the so-called depressor effect of corneal superinfection with ps. pyocyanea was tested. the experimental results showed that primary resistance of the mouse cornea against ps. pyocyanea could be reduced by local application of chloromycetin drops, but not by actinomycin d. likewise, the depressor effect o ... | 1977 | 304689 |
| the limulus lysate test. a rapid test for diagnosis of pseudomonas keratitis or endophthalmitis. | the limulus amebocyte lysate test has been shown to be a highly sensitive indicator of endotoxin. our studies showed that as little as 5 ng of endotoxin could be detected in aqueous or vitreous humor in vitro, although 10 microgram endotoxin injected into the aqueous could not be detected. subsequent studies showed that by diluting the aqueous equally with saline solution, this inhibitory effect could be overcome. detection of endotoxin elaborated from pseudomonas aeruginosa was made as early as ... | 1978 | 307380 |
| beta-lactamases and r-plasmids of haemophilus influenzae. | the emergence of resistance to ampicillin and other antibiotics in haemophilus influenzae has been a relatively recent event. in contrast, drug resistance has been rampant in the enterobacteriaceae for many years. ampicillin-resistance in h. influenzae is almost invariably attributable to possession of the tem (type iii a)beta-lactamase. as is common in other bacteria the gene specifying this enzyme is plasmid-borne in haemophilus. some ampicillin-resistant strains of h. influenzae can transfer ... | 1978 | 308259 |
| combined activitiy of clavulanic acid and ticarcillin against ticarcillin-resistant, gram-negative bacilli. | fifty-one clinical isolates of ticarcillin-resistant, gram-negative bacilli were tested for susceptibility to combinations of ticarcillin and clavulanic acid (brl 14151), a potent beta-lactamase inhibitor. minimal inhibitory concentrations (mics) were measured by a microdilution method, and minimal bactericidal concentrations for selected strains were measured by the broth dilution method. ticarcillin mics were >/=128 mug/ml for all and >/=512 mug/ml for 38 (75%) of the strains. thirty-nine stra ... | 1978 | 308792 |
| clavulanic acid, a novel inhibitor of beta-lactamases. | clavulanic acid, z-(2r,5r)-3-(beta-hydroxyethylidene)-7-oxo-4-oxa-1-azabicyclo-[3,2,0] heptane-2-carboxylic acid, has been shown to be an effective inhibitor of the beta-lactamases of the richmond types ii, iii, iv, and v. inhibition is a time-dependent reaction and is irreversible. clavulanic acid had poor antibacterial activity against staphylococcus aureus, enterobacteriaceae, and pseudomonas aeruginosa, with minimal inhibitory levels greater than 25 mug/ml. it did inhibit the majority of nei ... | 1978 | 310279 |
| implications of heterophile antigens in immunological infertility in males. | immunological study was conducted on serum and hydrocel fluids from males belonging to an equatorial african population heavily contaiminated by filaria and schistosoma. anti-spermatozoa antibodies were detected by agglutination in 31 of 64 sera and 24 of 60 fluids, and by cytotoxicity in 19 of 64 sera and 8 of 60 fluids. antinuclear antibodies were detected in 21 of 64 sera and 8 of 60 fluids. however, the titers of these antibodies were low, as were the frequency and titers of other tissues an ... | 1978 | 310662 |
| antibacterial activity of a new 1-oxa cephalosporin compared with that of other beta-lactam compounds. | the in vitro activity of (6r,7r)-7-{[carboxy(4-hydroxyphenyl)acetyl]amino}-7-methoxy-3-[[(1-methyl -1h-tetrazol-5-yl)thio]methyl]-8-oxo-5-oxa-1-azabicyclo-[4.2.0]oct-2-ene -2-carboxylic acid was tested against isolates of gram-positive and negative bacteria and compared with those of cephalothin, cefuroxime, cefamandole, cefoxitin, cefotaxime, and carbenicillin. the compound was less active than the other compounds when tested against staphylococcus aureus and staphylococcus epidermidis. it had ... | 1979 | 314774 |
| comparative activity and beta-lactamase stability of cefoperazone, a piperazine cephalosporin. | the in vitro activity and beta-lactamase stability of 7-[d(-)-alpha-(4-ethyl-2,3-dioxopiperazino-carbonylamino) -p-hydroxyphenylacetamido]-3-[(1-methyl)-5-tetrazolylthiomethyl] -delta(3)-cephem-4-carboxylic acid (cefoperazone), a cephalosporin analog of piperacillin, were compared with the activities and stabilities of other cephalosporins and cephamycins. the compound was less active than cephalothin or cefamandole in inhibiting staphylococcus aureus; it was as active as cefamandole and cefoxit ... | 1979 | 314775 |
| predictive value for survival of lymphocytes and polymorphonuclear leukocytes in patients with sepsis. | to study their value in predicting prognosis, tests were performed on peripheral blood lymphomononuclear cells and polymorphonuclear leukocytes in 34 critically ill patients with sepsis. intially, the number of lymphomononuclear cells was reduced by 32% compared with healthy control subjects and was 42% lower in those who died than in survivors. the values remained low in those who died. the numbers of t and b cells, determined by rosette formation using sheep and mouse erythrocytes, did not cha ... | 1979 | 315258 |
| ly-127935: a novel beta-lactam antibiotic with unusual antibacterial activity. | the in vitro activity of ly-127935, a new beta-lactam antibiotic, was examined by using 370 clinical bacterial strains. in comparison with several other beta-lactam agents, ly-127935 was the most inhibitory against the enterobacteriaceae. it was remarkably active against multi-drug-resistant strains of enterobacter spp., serratia spp., and pseudomonas aeruginosa. ly-127935 had four- to eightfold greater activity than did cefoxitin against bacteroides fragilis. production of beta-lactamase by ent ... | 1979 | 315753 |
| ceforanide (bl-s786) in the treatment of community-acquired bacterial pneumonia. | ceforanide (bl-s 786) is a new long-acting parenteral cephalosporin which has the major pharmacologic advantage of requiring only twice a day dosage. we treated 28 adult patients with community-acquired bacterial pneumonia using doses of 500 or 1000 mg every 12 hours. twenty-four of 28 infections were due to streptococcus pneumoniae and/or hemophilus influenzae, and all pathogens were susceptible in vitro to both cephalothin and ceforanide. patients were treated for a mean of 7.5 days, and all s ... | 1979 | 315929 |
| in vitro antibacterial activity and beta-lactamase stability of sce-129, a new cephalosporin. | sce-129 [3-4-carbamoyl-1-pyridiniomethyl-7beta- (d-alpha-sulfophenylacetamido)-ceph-3-em-4-carboxylate] is a cephalosporin that inhibits pseudomonas aeruginosa and staphylococcus aureus. sce-129 is tenfold more active than carbenicillin in inhibiting p. aeruginosa. sce-129 has poor activity against enterobacteriaceae compared with other cephalosporins and is 16-fold less active than the cephalosporins against streptococci. the activity of sce-129 does not correlate with beta-lactamase stability, ... | 1979 | 316683 |
| in vitro antibacterial activity of cefoperazone (t-1551), a new semisynthetic cephalosporin. | cefoperazone, a new semisynthetic cephalosporin, has a broad spectrum of antibacterial activity. it is as active as cefazolin and cefamandole against gram-positive bacteria and is more active than cefazolin and cefamandole against such gram-negative bacilli as escherichia coli, klebsiella pneumoniae, proteus species, pseudomonas aeruginosa, citrobacter freundii, enterobacter cloacae, and serratia marcescens. the superiority of cefoperazone over cefazolin and cefamandole with respect to activity ... | 1979 | 316988 |
| comparison of antibacterial activity of a new cephalosporin, ceftizoxime (fk 749) with other cephalosporin antibiotics. | fk 749 is a distinctive new parenteral cephalosporin antibiotic with a broad antibacterial spectrum which is more potently active against a wide variety of gram-negative bacilli, including the opportunistic pathogens such as citrobacter and enterobacter species and serratia marcescens, than sce 963, t 1551 and cefmetazole. the activity of fk 749 against escherichia coli, klebsiella pneumoniae, pyogenes was by far superior to that of the three other antibiotics. these test organisms were not resi ... | 1979 | 317284 |
| cefoxitin therapy for surgical patients. | cefoxitin was administered to 25 patients on a general surgical service. in 16 of these patients, a bacteriologic diagnosis was made: four patients had peritonitis, one had ascending cholangitis, seven had cellulitis, and four had abscess. the dosage of cefoxitin varied from 4 to 8 g per day and was generally well tolerated, although there were three cases of phlebitis and one superinfection. there was no evidence of renal, liver, or bone marrow toxicity. all isolates of bacteroides (eight), esc ... | 1979 | 318221 |
| clindamycin and gentamicin for aerobic and anaerobic sepsis. | thirty-eight adult patients with serious pleuropulmonary, soft-tissue, bone, and intra-abdominal infections caused by combinations of aerobic, facultative, and anaerobic bacteria were treated with parenterally given clindamycin phosphate and gentamicin sulfate and surgery when appropriate. nine had associated bacteremia. in 29, infections failed to respond to other therapeutic regimens, which included penicillins, cephalosporins, aminoglycosides, and chloramphenicol. results with clindamycin and ... | 1977 | 318824 |
| adjuvant protection against bacterial infection in granulocytopenic mice. | the hypothesis that the induction of nonspecific resistance to infection by immunostimulation prior to drug-induced granulocytopenia would afford increased protection to subsequent bacterial challenge was tested in a murine model of infection with pseudomonas aeruginosa or staphylococcus aureus in mice rendered granulocytopenic with cyclophosphamide. prior intraperitoneal immunostimulation of mice with complete freund's adjuvant (cfa) or mycobacterium bovis (bacille calmette-guèrin; bcg) increas ... | 1977 | 328788 |
| host factor for coliphage q beta rna replication: presence in procaryotes and association with the 30s ribosomal subunit in escherichia coli. | the host factor required for in vitro coliphage q beta rna replication, a heat-stable rna binding protein present in uninfected escherichia coli, has been detected by both immunological and functional tests in acinetobacter calcoaceticus, klebsiella pneumoniae, pseudomonas aeruginosa and pseudomonas putida. it was not detectable by these criteria in bacillus stearothermophilus, bacillus subtilis, caulobacter crescentus, micrococcus lysodeikticus, rhodopseudomonas capsulata or saccharomyces cerev ... | 1977 | 329101 |
| antibody to cell wall glycolipid of gram-negative bacteria: induction of immunity to bacteremia and endotoxemia. | antiserum to the core glycolipid of gram-negative bacteria was prepared by immunization of rabbits with vaccine composed of killed cells of the uridine diphosphate galactose-deficient mutant (j5) of escherichia coli o:111. antiserum to j5 not only prevented death of animals from endotoxin but also prevented the local and generalized shwartzman reactions. antiserum to endotoxin also prevented renal cortical necrosis and disseminated intravascular coagulation during the evolution of the generalize ... | 1977 | 330776 |
| the time at which infected postoperative wounds demonstrate increased strength. | it was again shown in this study that laparotomy wounds purposely infected with a known inoculum of live gram-negative bacteria exhibited greater tensile strength than did those in the normal control group. the organisms used were escherichia coli and pseudomonas aeruginosa. the phenomenon was not present during the first ten days after wounding but was quite evident in wounds tested at 14 and 21 days. by histologic examination, there was evidence of much more new collagen in the wounds that exh ... | 1977 | 333641 |
| acriflavine violet red bile agar for the isolation of klebsiella. | a medium for the isolation and detection of klebsiella is described. it contains 0.06% acriflavine in violet red bile agar (difco). klebsiella appeared as 5 to 7 mm mucoid (24 h at 37 degrees c) golden-yellow colonies. pseudomonas aeruginosa and enterobacter spp. appeared as small, brown to dark brown colonies. escherichia coli as well as many other gram negative organisms and gram positive organisms did not grow on this medium. | 1977 | 334697 |
| preprototype of an automated microbial detection and identification system: a developmental investigation. | the automicrobic system is an automated, computerized instrument that uses highly selective media and an optical system for detection, enumeration, and identification of bacteria and some yeasts in 13 h. a preprototype instrument (automicrobic system-1) and its urine culture kit (identi-pak), developed for the detection, enumeration, and identification of eight species or groups of bacteria and of candida species and torulopsis glabrata in urine specimens, was evaluated during its development. a ... | 1977 | 334797 |
| automated microbiological detection/identification system. | an automated, computerized system, the automicrobic system, has been developed for the detection, enumeration, and identification of bacteria and yeasts in clinical specimens. the biological basis for the system resides in lyophilized, highly selective and specific media enclosed in wells of a disposable plastic cuvette; introduction of a suitable specimen rehydrates and inoculates the media in the wells. an automated optical system monitors, and the computer interprets, changes in the media, wi ... | 1977 | 334798 |
| clinical and bacteriological evaluation of netilmicin in gram-negative infections. | a preliminary study was conducted with netilmicin, a new aminoglycoside antibiotic. its effectiveness was evaluated in vitro against 546 strains of enterobacteriaceae and pseudomonas aeruginosa isolated from clinical material. its effectiveness against gentamicin-susceptible strains was roughly similar to that of gentamicin and other newer aminoglycoside antibiotics. cross-resistance to netilmicin was found in 9 of 44 gentamicin-resistant strains. a favorable clinical response was observed in 70 ... | 1977 | 335960 |
| inhibition of acetylating activity in an enzymatic assay for aminoglycoside antibiotics. | the radioenzymatic technique using aminoglycoside-6'-acetyltransferase is suitable for determining serum concentrations of aminoglycoside antibiotics that have a 6'-amino group. standard curves constructed for each drug in normal human serum are generally satisfactory, but we encountered a pool of human serum (designated in pool) that inhibited the acetylating activity of the assay. standard curves for amikacin, kanamycin, gentamicin, and tobramycin in the acetylating assay were all abnormal wit ... | 1977 | 335965 |
| amikacin and cephalothin: empiric regimen for granulocytopenic cancer patients. | amikacin (15 mg/kg per day) was used in combination with cephalothin (7 g/m(2) per day) as an empiric regimen for de novo febrile (>101 degrees f [38.3 degrees c]) episodes in 93 granulocytopenic (<1,000/mm(3)) cancer patients. both drugs were given intravenously in four equal doses every 6 h. the response rate for all documented infections was 83%, including 11 of 17 (65%) bacteremias. escherichia coli (14 cases) was the most common pathogen, whereas pseudomonas aeruginosa (2 cases) caused fewe ... | 1977 | 335967 |
| antimicrobial agents--part ii. the aminoglycosides: streptomycin, kanamycin, gentamicin, tobramycin, amikacin, neomycin. | aminoglycoside antibiotics are poorly absorbed from the gastrointestinal tract, do not penetrate well into the cerebrospinal fluid, are minimally bound to plasma proteins, and are rapidly excreted by the normal kidney. neomycin is limited by its toxicity to irrigating and topical preparations or to oral medication for surgical bowel preparations or hepatic coma. streptomycin has only a few specific indications, because newer agents are available that have broader spectrums of activity. kanamycin ... | 1977 | 336988 |
| corneal rings with gram-negative bacteria. | corneal rings have been described with corneal ulcerations caused by gram-negative bacteria. corneal rings were produced by intracorneal injections of viable pseudomonas aeruginosa as well as heat-inactivated suspensions of gram-negative bacteria (p aeruginosa and escherichia coli) but not gram-positive bacteria (staphylococcus aureus) or freund's adjuvant. it is suggested that endotoxin is the factor responsible for their production since purified endotoxin produced corneal rings after intracor ... | 1977 | 337946 |
| nocardicin a, a new monocyclic beta-lactam antibiotic v. in vivo evaluation. | nocardicin a is a new monocyclic beta-lactam antibiotic which provides a potent therapeutic effect in mice experimentally infected with gram-negative bacilli. when given subcutaneously to mice, the therapeutic effect of the drug was stronger than had been anticipated from in vitro studies. nocardicin a was more potent in therapeutic effect than carbenicillin against infections due to pseudomonas aeruginosa, proteus mirabilis, pr. vulgaris, pr. rettgeri and pr. inconstans, and was similar in effe ... | 1977 | 338567 |
| nitramino acids. synthesis and biological evaluation of 1-nitroproline, 1-nitropipecolic acid, and n-nitrosarcosine. | the n-nitro derivatives of secondary alpha-amino acids, viz., 1-nitroproline (1a) (l and d), 1-nitro-dl-pipecolic acid (2a), and n-nitrosarcosine (3a), were prepared by the oxidation of the corresponding nitrosamino acids with peroxytrifluoroacetic acid. these nitramino acids (1a-3a) were not active against escherichia coli, candida albicans, pseudomonas aeruginosa, or mycobacterium smegmatis, and 1a and 2a did not show mutagenic activity in a salmonella typhimurium ta-100 system, with or withou ... | 1977 | 338899 |
| evaluation of a multitest system for identification of saccharolytic pseudomonads. | the api-20 enteric strip has been proposed to have the capacity to differentiate the nonfermentative gram-negative bacilli. this multitest strip serves as the basis of an evolving system, which first provided a register, then a selector, and more recently a computer index. twenty-three strains of pseudomonas aeruginosa and p. putida, 21 of p. fluorescens, 27 of p. maltophilia, and 33 of p. cepacia were investigated by duplicate test runs to determine reproducibility. the results were then evalua ... | 1978 | 339710 |
| qualitative study of paradoxical zone phenomenon of penicillins against 17 bacterial species of clinical importance. | using a triple agar layer technique and enzymatic inactivation of penicillin, the occurrence of a paradoxical zone phenomenon (illustrated by a typical 'target' image around the reservoir of antibiotic) was determined for several bacterial species specially chosen with regards to their taxonomic position, clinical importance and penicillin susceptibility. among gram-positive bacteria, a paradoxical zone was obtained for approximately 43% of the strains studied here (all 10 strains of staphylococ ... | 1978 | 340171 |
| skin protothecosis in a patient with renal allograft. | the case history of a 30-year-old male kidney transplant patient who developed cutaneous protothecosis is presented. the lesions, initially located over the forearm and around the site of previous av shunt, consisted of maculopapular areas which opened spontaneously and drained purulent sanguineous material. culutre of drainage grew prototheca wickerhamii. other virulent organisms, ie. klebsiella pneumoniae and pseudomonas aeruginosa, were also grown. the patient died of klebsiella septicemia an ... | 1978 | 341343 |
| fever and infection in leukemic patients: a study of 494 consecutive patients. | all of the febrile episodes occurring in 494 adults with acute leukemia were reviewed. there were an average of 2.39 febrile episodes per patient and the patients spent 28% of their days in the hospital with fever. sixty-four percent of the febrile episodes were due to infection. the most common types of infection were disseminated infection and pneumonia, which together accounted for 69% of the total episodes of documented infection. the etiologic agent was identified in 73% of the documented ... | 1978 | 346201 |
| increased antimetabolite sensitivity with variation of carbon source during growth. | in serratia marcescens, analogs of leucine (norleucine), methionine (alpha-methylmethionine), histidine (3-amino-1,2,4-triazolealanine), tyrosine (p-aminophenylalanine), and tryptophan (7-methylindole) are conditional inhibitors of growth; inhibition occurs during the metabolism of some carbon sources but not with others. a further increase in sensitivity to growth inhibition by these analogs can be accomplished through the use of particular combinations of carbon sources present in the inoculum ... | 1978 | 346563 |
| [in vitro activity of cefoxitin compared with the activity of other antibiotics and trimethoprim-sulfamethoxazole (author's transl)]. | the new developed antibiotic cefoxitin has been tested in vitro on its effectiveness against bacteria isolated from human material. pseudomonas aeruginosa and streptococci of the serological group d were not tested, because these microorganisms are not sensitive against this antibiotic. besides cefoxitin other antibiotics (cephalothin, cephalexin, ampicillin, carbenicillin, tetracycline, gentamicin, penicillin g, oxacillin) and trimethoprim-sulfamethoxazole were also tested. as to the results ce ... | 1978 | 347234 |
| l-arabinose-ornithine-irgasan medium for differentiating serratia species. | a semisolid medium (designated serratia differentiation medium) containing l-arabinose, ornithine, and selective inhibitor was used to differentiate three clinically encountered serratia species. the inhibitor, irgasan dp-300, was incorporated to eliminate false-positive reactions from most remaining enterobacteriaceae. the suspected serratia colony was inoculated as a stab into the medium. serratia marcescens was indicated by a change in color from olive to purple following 18 h of incubation, ... | 1978 | 348720 |
| performance of converted pressure cookers and two conventional jars for anaerobic bacterial culture. | the simple conversion of commercial pressure cookers into inexpensive anaerobic jars is described. these containers were shown to be as good as the small conventional bbl polycarbonate gaspak and large vented 150 gas-replacement jars when assessed by means of three biological indicators: pseudomonas aeruginosa, bacteroides melaninogenicus, and bacteroides fragilis. ps. aeruginosa seeded on simmond's citrate agar was shown to be the most sensitive indicator of the three for traces of oxygen. | 1978 | 348726 |
| pathogenesis of bacteremia due to pseudomonas aeruginosa in cyclophosphamide-treated mice and potentiation of virulence of endogenous streptococci. | mice treated with cyclophosphamide on day 0 were killed at sequential intervals during subsequent administration of pseudomonas aeruginosa in drinking water and were examined for sites of bacterial colonization and multiplication with use of peroxidase-labeled antibody and culture techniques. these studies were coordinated with those of clinical and histopathologic changes. bacterial antigen was first detected on day 4 on the surface of nasal squamocolumnar junctions and the stratum corneum of g ... | 1978 | 351090 |
| evaluation of the oxi/ferm tube system for identification of nonfermentative gram-negative bacilli. | the oxi/ferm tube system designed for identification of oxidative-fermentative gram-negative bacteria was evaluated for identification of nonfermentative gram-negative bacilli by comparing it with the conventional system. the nine biochemical reactions used as the initial tests in the oxi/ferm tube system showed a close agreement with the same conventional reactions. however, the system was only 41% accurate to species or biotype for complete identification of 239 isolates which were well distri ... | 1978 | 353066 |
| [microbiological examinations in the intensive surgical care unit of the general calixto garcia teaching hospital]. | microbiological examinations carried out in the intensive surgical-care unit of the "calixto garcía" teaching hospital between october, 1974 and february, 1976 are exposed. nineteen examinations were carried out in the unit and results were as follows: 53,1% of the personnel had pathogenic germs (43,8%, coagulase-positive staphylococci; 6,3%, beta-hemolytic streptococci; and 6,3%, klebsiella). acceptable results were obtained in the unit air but it was not the case in laboratory and nursery sect ... | 1977 | 354643 |
| [serratia marcescens, pseudomonas aeruginosa and urinary tract infections]. | | 1978 | 357787 |
| nonvalue of neomycin instillation after intermittent urinary catheterization. | this study evaluated weekly urine cultures of patients with neurogenic bladder disease who underwent intermittent urinary catheterization for bladder retraining. one group of 53 patients in 1974 received regular instillations of 0.1% neomycin after each catheterization. a similar group of 55 patients in 1975 did not receive neomycin and constituted a control group. distribution of age, sex, diagnosis, and duration of bladder retraining was comparable in both groups. quantitative bacterial colony ... | 1978 | 360984 |