| deletion plasmids from transformants of pseudomonas aeruginosa trp cells with the rsf1010-trp hybrid plasmid and high levels of enzyme activity from the gene on the plasmid. | a rsf1010-trp hybrid plasmid which contained the tryptophan operon of escherichia coli was introduced into pseudomonas aeruginosa trp cells by transformation. from the trp+ transformants several deletion plasmids were obtained, and their physical maps with restriction endonucleases were constructed. p. aeruginosa trp cells with these plasmids showed at first more than 100 times higher levels of tryptophan synthetase beta activity over that of the control p. aeruginosa wild-type cells, but these ... | 1978 | 101514 |
| heat-stable somatic antigens of a group of unclassified fluorescent pseudomonads (ufp). | (i) isolates belonging to a group of unclassified fluorescent pseudomonads (ufp) are similar to pseudomonas aeruginosa not only in cultural behaviour but also in basic serological properties of their somatic antigens. living bacteria and cultures subjected to prolonged heating at 100 degrees c or above agglutinated readily in homologous o serum, whereas after exposure to 60 degrees c, ethanol, saturated sodium chloride and formalin the cells of both species became practically inagglutinable. sur ... | 1979 | 94240 |
| linkage map of pseudomonas aeruginosa pat. | the locations of new markers relative to markers previously mapped on the chromosome of pseudomonas aeruginosa strain pat were defined by generalized transduction with phage f116l and f1083. although the marker orders of the various marker groups were deduced mainly from the results of two-factor crosses, the locations of a number of markers were confirmed by three-factor crosses. a linkage map of the chromosome of p. aeruginosa pat was constructed which shows the relative locations of 50 genes. ... | 1978 | 101525 |
| relationship between catabolism of glycerol and metabolism of hexosephosphate derivatives by pseudomonas aeruginosa. | the relationship between catabolism of glycerol and metabolism of hexosephosphate derivatives in pseudomonas aeruginosa was studied by comparing the growth on glycerol and enzymatic constitution of strain pao with these characteristics of glucose-catabolic mutants and revertants. growth of strain pao on glycerol induced a catabolic oxidized nicotinamide adenine dinucleotide-linked glyceraldehyde-phosphate dehydrogenase and seven glucose-catabolic enzymes. the results indicated that these enzymes ... | 1978 | 101528 |
| mezlocillin: a new acyl ureidopenicillin. antimicrobial activity and combination effects with four aminoglycoside antibiotics. | mezlocillin (baypen), carbenicillin and mezlocillin combined with gentamicin, sisomicin, tobramycin and amikacin, respectively, were tested in vitro against 325 fresh clinical isolates of pathogenic gram-negative bacteria using the tube dilution procedure. the new acyl ureidopenicillin has a greater antimicrobial activity than carbenicillin against nearly all species tested. the comparison of the effectiveness of the mezlocillin-aminoglycoside combinations demonstrates the superiority of mezloci ... | 1978 | 95925 |
| effectiveness of immunization with single and multi-component vaccines prepared from a common antigen (oep), protease and elastase toxoids of pseudomonas aeruginosa on protection against hemorrhagic pneumonia in mink due to p. aeruginosa. | toxoids of protease and elastase of pseudomonas aeruginosa were successfully prepared by treatment with 8% formalin plus 0.2 m lysine and by 4% formalin respectively. the two toxoids proved sufficiently potent to elicit high antibody titers as estimated by both the enzyme-neutralizing and passive hemagglutination tests. the effectiveness of immunizing minks with a single component-vaccine consisting of the common antigen (oep) of p. aeruginosa or the protease toxoid (pt) or the elastase toxoid ( ... | 1978 | 101698 |
| studies on the detoxification of pseudomonas aeruginosa exotoxin. | | 1978 | 101699 |
| l-form of pseudomonas aeruginosa. 1. an effective method for the production of stable l-forms. | | 1978 | 101700 |
| the role of oep-antibodies in pseudomonas aeruginosa infection. | when mice were challenged intraperitoneally with 2.2 x 10(4) pseudomonas aeruginosa in 0.5 ml of 2.7% mucin solution, the bacteria markedly increased in the peritoneal cavity, rapidly entered the blood stream, and the mice died, probably of bacteremia, within a short time. however, when mice were injected subcutaneously 4 hours prior to the challenge with 0.2 ml of 0.75% igg (oep-ha = 12.8) or igm (oep-ha = 64) separated from human plasma or 3% s-iga ep-ha = 19.2) from fresh human milk, the bact ... | 1978 | 101701 |
| l-form of pseudomonas aeruginosa. 2. antibiotic sensitivity of l-forms and their parent forms. | | 1978 | 101702 |
| effect of certain sulphydryl compounds and dimethyl sulphoxide on potassium release by certain amines and proteins from pseudomonas aeruginosa. | dithiothreitol, cysteine, and mercapto-ethanol inhibit k+ release from cells of pseudomonas aeruginosa by amines and nh4cl incubated at ph 8.5. the inhibitions are competitive for the monamines and nh4cl but non-competitive for the diamines and spermidine. the percent inhibition is largest for tryptamine, least for spermidine and ethylene diamine. dmso inhibits k+ release by monamines and certain proteins but not significantly by diamines and histamine. the inhibitions are competitive. | 1977 | 101739 |
| [natural immunity. phagocytosis of pseudomonas aeruginosa in guinea pigs determined in vitro]. | | 1978 | 102011 |
| [lysine biosynthesis in pseudomonas aeruginosa pao 1. ii. first studies of dap decarboxylase of lysine auxotrophic mutant of p. aeruginosa pao 1]. | | 1978 | 102088 |
| pattern of phenazine pigment production by a strain of pseudomonas aeruginosa. | an atypical strain of pseudomonas aeruginosa capable of synthesizing three phenazine pigments was isolated. cultural conditions, under which the strain forms either chlororaphin, oxychlororaphin, or pyocyanine, are described. this broad spectrum of pigment production, as well as some other characteristics, sets this strain apart from previously described chlororaphin producers. | 1978 | 96094 |
| characterization of pseudomonas aeruginosa mutants deficient in the establishment of lysogeny. | mutants of pseudomonas aeruginosa with impaired ability to establish a lysogenic relationship with temperate bacteriophage (les-) have been isolated. these les mutations map to two areas of the p. aeruginosa chromosomal map as determined by conjugational and transductional analyses. two phenotypic classes of les- mutants were identified. one class of mutations has pleiotropic effects on dna metabolism. these mutants are unable to recombine genetic material acquired as a result of either conjugat ... | 1978 | 96103 |
| new antipseudomonal penicillin, pc-904: affinity to penicillin-binding proteins and inhibition of the enzyme cross-linking peptidoglycan. | the mechanism of action of a new antipseudomonal penicillin, pc-904, was studied with respect to its binding affinities to penicillin-binding proteins (pbps) and its inhibitory activities on cross-linking enzymes of peptidoglycan synthesis in vitro. pc-904 showed especially high affinity (compared with that of penicillin g) to escherichia coli pbp-3. it also had high affinities to pbp-2 and -1bs and low affinities to pbp-1a, -4, -5, and -6. similar results were obtained with pseudomonas aerugino ... | 1978 | 102247 |
| [pseudomonas aeruginosa epidemiology in egypt: study of 80 hospital strains isolated at alexandria]. | eighty hospital strains of p. aeruginosa, isolated in alexandria, were examined with the aid of three epidemiological markers (serotype, lysotype and o. n. p. g.-test). the most frequent serotypes in alexandria are 0:11, 0:12, 0:2, 0:4, 0:6 et 0:1; this situation is different from those reported in european countries and in u. s. a. sixty-five lysotypes were found and the strains of serotype 0:11 fall in fifteen lysotypes. one strain 0:12 was found o. n. p. g. positive. | 1978 | 102436 |
| in vitro activity of hr 756, a new cephalosporin compound. | the in vitro activity of hr 756, a new cephalosporin, has been determined against recent clinical isolates and compared with that of other beta-lactam antibiotics. the geometric means of the minimum inhibitory concentrations (mic) for different isolates of escherichia coli (100 isolates), klebsiella pneumoniae (84), pseudomonas aeruginosa (121), proteus mirabilis (52), indole-positive proteus species (9), salmonella species (19), staphylococcus aureus penicillin-sensitive (29) and penicillin-res ... | 1978 | 102631 |
| an explanation for the apparent host specificity of pseudomonas plasmid r91 expression. | pseudomonas aeruginosa strain 9169 has been reported to contain a plasmid that expresses resistance to carbenicillin (cb), kanamycin (km), and tetracycline (tc) in escherichia coli but resistance only to cb in certain pseudomonas recipients. the triply resistant plasmid in e. coli belonged to incompatibility (inc) group p or p-1, whereas the singly resistant plasmid in p. aeruginosa was compatible with incp-1 plasmids and other plasmids of established inc specificity but incompatible with plasmi ... | 1978 | 102634 |
| comparative toxicities of diphtherial toxin and pseudomonas aeruginosa exotoxin a: evidence for different cell receptors. | | 1978 | 102734 |
| [appearance of various pyocin types and subtypes of pseudomonas aeruginosa in the same patient]. | | 1978 | 100965 |
| [a study of serotypes of pseudomonas aeruginosa isolated in man in saigon (vietnam) (author's transl)]. | the authors study the serological distribution of 143 strains of pseudomonas aeruginosa isolated in hospital grall (saigon-vietnam) according to the country (south vietnam), the kind of pathological specimens and the various departments of the hospial. they describe a special distribution of serological groups in vietnam with a large predominance of groups 0 = 6 and 0 = 11. they consider the possibility of adapting a polyvalent vaccine including the 10 serological groups now prevailing in vietna ... | 1978 | 102901 |
| pseudomonas cytochrome c551 at 2.0 a resolution: enlargement of the cytochrome c family. | the structure of respiratory cytochrome c551 of pseudomonas aeruginosa, with 82 amino acids, has been solved by x-ray analysis and refined to a crystallographic r factor of 16.2%. it has the same basic folding pattern and hydrophobic heme environment as cytochromes c, c2, and c550, except for a large deletion at the bottom of the heme crevice. this same "cytochrome fold" appears to be present in photosynthetic cytochromes c of green and purple sulfur bacteria, and algal cytochromes f, suggesting ... | 1978 | 96440 |
| plasmid-determined resistance to hexachlorophene in pseudomonas aeruginosa. | plasmid-containing strains of pseudomonas aeruginosa were screened for resistance to disinfectants. two plasmids, pmg1 and pmg2, were found to determine hexachlorophene resistance. | 1978 | 96729 |
| plasmid-determined resistance to boron and chromium compounds in pseudomonas aeruginosa. | plasmids determining resistance to arsenic, mercury, silver, and tellurium compounds in escherichia coli and pseudomonas aeruginosa were tested for resistance to 40 other metal compounds. resistance to trivalent boron and hexavalent chromium compounds was a property of certain p. aeruginosa plasmids. | 1978 | 96730 |
| beta-lactamases: determination of their isoelectric points. | important discrepancies in isoelectric points (pi) of beta-lactamases were observed depending on the experimental procedure used for their determination: isoelectric focusing (ief) in sucrose density gradients or analytical ief in thin-layer polyacrylamide gels (pa). the variations, negligable in the case of tem-like beta-lactamases, appeared to be important in the case of cephalosporinases and are related to an artifact which appears in pa-ief. this has been clearly shown with beta-lactamase pr ... | 1978 | 96731 |
| recurrent pseudomonas infection associated with neutrophil dysfunction. | a 72-year-old male is described with a history of 4 episodes of pseudomonas aeruginosa sepsis and chronic otitis media caused by pseudomonas species. in vitro testing of the patient's polymorphonuclear leukocytes (pmns) revealed profoundly abnormal chemotactic responses and defective intracellular killing of ps. aeruginosa, staphylococcus aureus and escherichia coli. chemiluminescence production by the patient's pmns in response to opsonized zymosan as well as endotoxin stimulated nitroblue tetr ... | 1978 | 103188 |
| [susceptibility of pseudomonas aeruginosa in relation to the methods of investigation]. | | 1978 | 103321 |
| [research in the stability of pyocin-types and subtypes in pseudomonas aeruginosa]. | | 1978 | 103322 |
| [sensitivity of bacteria to antibiotics. evaluation of a rapid automatic method (author's transl)]. | a rapid automated sensitivity testing system is evaluated in this report. the results have been compared with the standard disk diffusion method (ics). three hundred strains recently isolated from clinical cases : enterobacteriaceae, staphylococcus aureus, streptococci d, pseudomonas aeruginosa, have been tested. the general agreement between the two techniques was 89 p. cent. | 1978 | 97613 |
| antibodies to proteases and exotoxin a of pseudomonas aeruginosa in patients with cystic fibrosis: demonstration by radioimmunoassay. | sera from 33 patients with cystic fibrosis and two pediatric patients being treated for chronic pulmonary infections not related to cystic fibrosis and six sera or serum pools from uninfected individuals were tested with a microtiter radioimmunoassay for reactivity against exotoxin a and two proteases from pseudomonas aeruginosa. exotoxin a was purified from a low-protease strain of p. aeruginosa and shown to have adenosine diphosphate-ribose transferase activity and mouse lethality. proteases w ... | 1978 | 98596 |
| a comparison of media used for the early detection of the bluish-green pigment of pseudomonas aeruginosa. | | 1978 | 103694 |
| comparison of silver sulfadiazine and gentamicin for topical prophylaxis against burn wound sepsis. | daily prophylactic application of either 1.0% silver sulfadiazine cream or 0.1% gentamicin cream was compared for effectiveness in preventing bacterial colonization of burn wounds and sepsis. pseudomonas aeruginosa colonized the wounds of 37% of the 38 patients treated with silver sulfadiazine and 30% of the 33 patients treated with gentamicin; gentamicin-resistant p. aeruginosa colonized the wounds of 21% of the patients treated with gentamicin. staphylococcus aureus colonization occurred in 55 ... | 1978 | 99223 |
| beta-lactamase stability of hr 756, a novel cephalosporin, compared to that of cefuroxime and cefoxitin. | the stability to beta-lactamase hydrolysis of hr 756, a new cephalosporin antibiotic, was compared to the beta-lactamase stability of cefoxitin and cefuroxime. hr 756, cefoxitin, and cefuroxime were not hydrolyzed by richmond type i, iii, iv, and v beta-lactamases. antibacterial activity of hr 756 correlated well with resistance to beta-lactamase hydrolysis except against pseudomonas aeruginosa. hr 756, cefoxitin, and cefuroxime inhibited type i beta-lactamases, but not type iii, iv, or v enzyme ... | 1978 | 101128 |
| effect of different lots of mueller-hinton agar on the interpretation of the gentamicin susceptibility of pseudomonas aeruginosa. | population distributions and quality control data for strains of pseudomonas aeruginosa tested for gentamicin susceptibility on six lots of mueller-hinton agar were analyzed. the lots of agar were used in three university of washington hospitals from april 1975 through october 1977. the analyses indicated that the performance of members of the p. aeruginosa populations in each hospital closely followed the performance of the quality control strain, p. aeruginosa atcc 27853, when tested on each l ... | 1978 | 101130 |
| analysis of the interactions between piperacillin, ticarcillin, or carbenicillin and aminoglycoside antibiotics. | enhanced activity against clinical isolates of pseudomonas aeruginosa was demonstrated with piperacillin, ticarcillin, and carbenicillin in combination with gentamicin, tobramycin, and amikacin. | 1978 | 101133 |
| proposed ticarcillin disk control values for escherichia coli and pseudomonas aeruginosa: multicenter cooperative study. | in a multicenter cooperative controlled study, individual test, accuracy, and precision control values were determined for 75-mug ticarcillin disks with escherichia coli atcc 25922 and pseudomonas aeruginosa atcc 27853. | 1978 | 101134 |
| in vitro comparison of kanamycin, kanendomycin, gentamicin, amikacin, sisomicin, and dibekacin against 200 strains of pseudomonas aeruginosa. | the antimicrobial activity of kanamycin, kanendomycin, gentamicin, amikacin, sisomicin, and dibekacin against 200 strains of pseudomonas aeruginosa was compared. dibekacin was found to be the most active against the tested organisms, whereas the other aminoglycoside antibiotics fell in the following order of diminishing antibacterial potency: amikacin, sisomicin, gentamicin, kanendomycin, and kanamycin. seven strains showed high-level resistance to gentamicin (minimal inhibitory concentration, 4 ... | 1978 | 101135 |
| pseudomonas ribosomal vaccines: preparation, properties, and immunogenicity. | the preparation, properties, and immunogenicity of ribosomal vaccines from pseudomonas aeruginosa are described. these preparations, containing protein and rna, were tested for immunogenicity by active immunization of mice and subsequent challenge with homologous, live bacteria. the results demonstrated that vaccines prepared from a majority of serotypes used were immunogenic, i.e., afforded 60 to 100% mouse protection against a challenge inoculum containing 8 to 50 50% lethal doses. in some cas ... | 1978 | 101464 |
| outer membranes of gram-negative bacteria. xix. isolation from pseudomonas aeruginosa pao1 and use in reconstitution and definition of the permeability barrier. | a method for separating the outer and inner membranes of pseudomonas aeruginosa pao1 in the absence of added ethylenediaminetetraacetic acid was devised. the method yields two outer membrane fractions which show the same protein pattern on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, but differ substantially in their relative contents of phospholipids. one of these outer membrane fractions and the inner membrane fraction are less than 4% cross-contaminated, as judged by the content ... | 1978 | 101518 |
| a test for 'hygienic' hand disinfection. | a standardised test procedure is described in which finger-tips are inoculated with broth cultures of organisms (staphylococcus aureus, staphyloccocus saprophyticus, escherichia coli, and pseudomonas aeruginosa): counts are made from washings of hands after disinfection with various antiseptic-detergents, alcoholic solutions, or unmedicated soap. 70% alcohol, with or without chlorhexidine, was the most effective preparation. the two antiseptic detergents showed variable results, but against gram ... | 1978 | 101554 |
| relationship between mutant amidases of pseudomonas aeruginosa and hydroxyurea as an inhibitor. | hydroxyurea inhibited growth of pseudomonas aeruginosa strain ai 3 on media containing either acetanilide (n-phenyl acetamide) or acetamide as sole carbon sources. mutants resistant to hydroxyurea inhibition of growth on acetanilide (ouch strains) and acetamide (amouch strains) displayed altered growth properties on various amide media compared with the parent strain ai3. ai3 amidase, which catalyses the initial step in the metabolism of acetanilide and acetamide, was inhibited by hydroxyurea in ... | 1978 | 104140 |
| chromosomal location of genes participating in the degradation of purines in pseudomonas aeruginosa. | genetic mapping of the genes (puu) that encode the enzymes catalysing degradation of purines in pseudomonas aeruginosa strain pao has been carried out. mutants that are deficient in adenine deaminase (puua), guanine deaminase (puub), xanthine dehydrogenase (puuc), uricase (puud), allantoinase (puue), and/or allantoicase (puuf) were isolated and used for the genetic study. conjugation by fp5 factor and generalized transduction by phage g101 gave the following map locations of these six genes on t ... | 1978 | 104142 |
| comparison of the in vitro activity of bay k 4999 and piperacillin, two new antipseudomonal broad-spectrum penicillins, with other beta-lactam drugs. | bay k 4999 and piperacillin, two new substituted ampicillins, were compared with other beta-lactam antibiotics, including carbenicillin, azlocillin, mezlocillin, benzylpenicillin, ampicillin, and cefoxitin, against a wide range of gram-positive and -negative organisms. bay k 4999 and piperacillin were extremely active against pseudomonas aeruginosa (50% inhibited by 2 mug/ml), being about 16-fold more active than carbenicillin. bay k 4999 was the most active drug against escherichia coli (50% in ... | 1978 | 102245 |
| [flagella specific h antigenic schema of pseudomonas aeruginosa (author's transl)]. | serogrouping of p. aeruginosa on the basis of heat-stable somatic (o-)agglutinogens is well established, but h-typing on the basis of heat-labile agglutinogens is not accepted generally because of uncertain flagella specificity. in contrast to the agglutination reaction, the indirect fluorescent antibody technique allows the differentiation of soma and flagellum of p. aeruginosa cells at the morphological and serological level simultaneously, and thereby the specific analysis of flagellar (h-)an ... | 1978 | 104481 |
| [l-forms and pigment production in "pseudomonas aeruginosa" (author's transl)]. | | 1978 | 104666 |
| [microbiological studies on bacterial spectra of milk samples from healthy udder quarters and from those with increased cell counts and/or conductivity values]. | the germ levels of 2,182 milk samples obtained from udder quarters with subclinical mastitis were compared to milk sampled from 2,061 udder quarters with physiological cell counts or conductivity values. three cattle herds were involved in the test programme. no germ growth was established from 9.5 per cent of all samples taken from udder quarters with increased cell counts and conductivities and from 4.1 per cent of those samples taken from intact udder quarters. samples taken from udder quarte ... | 1978 | 104694 |
| pulmonary function and morbidity in 40 adult patients with cystic fibrosis. | pulmonary function and cardiopulmonary complications were studied in a group of 40 patients with cystic fibrosis who reached the age of 25 years. mean values for vital capacity (vc), functional residual capacity, residual volume (rv), the ratio of rv over total lung capacity (rv/tlc), conductance, and the ratio of the forced expiratory volume in one second over vc were abnormal. there was a variable pattern of progression from patient to patient. the men differed from the women only in that they ... | 1978 | 104832 |
| effectiveness of gentamicin and carbenicillin in a rat model against infections with pseudomonas aeruginosa resistant to gentamicin or gentamicin and carbenicillin. | | 1979 | 104952 |
| chemotaxis by pseudomonas aeruginosa. | chemotaxis by pseudomonas aeruginosa rm46 has been studied, and conditions required for chemotaxis have been defined, by using the adler capillary assay technique. several amino acids, organic acids, and glucose were shown to be attractants of varying effectiveness for this organism. ethylenediaminetetraacetic acid was absolutely required for chemotaxis, and magnesium was also necessary for a maximum response. serine taxis was greatest when the chemotaxis medium contained 1.5 x 10(-5) m ethylene ... | 1979 | 104961 |
| isolation of an iron-binding compound from pseudomonas aeruginosa. | an iron-binding compound was isolated from ethyl acetate extracts of culture supernatant fluids of pseudomonas aeruginosa and was purified by successive paper and thin-layer chromatographic procedures. the purified compound was characterized by uv, visible, infrared, and fluorescence spectroscopy. the compound possesses phenolic characteristics, with little or no similarity to dihydroxybenzoates and no indication of a hydroxamate group. p. aeruginosa synthesized the compound during active growth ... | 1979 | 104968 |
| homology of the gene coding for outer membrane lipoprotein within various gram-negative bacteria. | the mrna for a major outer membrane lipoprotein from escherichia coli was found to hybridize specifically with one of the ecori and one of the hindiii restriction endonuclease-generated fragments of total dna from nine bacteria in the family enterobacteriaceae: e. coli, shigella dysenteriae, salmonella typhimurium, citrobacter freundii, klebsiella aerogenes, enterobacter aerogenes, edwardsiella tarda, serratia marcescens, and erwinia amylovora. however, among the enterobacteriaceae, dna from two ... | 1979 | 104972 |
| effect of pyocin r1 on the glucose metabolism of sensitive cells of pseudomonas aeruginosa. | the effect of pyocin r1 on the glucose metabolism of sensitive pseudomonas cells was investigated. upon treatment with pyocin r1, although the rate of o2 uptake of the sensitive cells for glucose or gluconate was not very much affected at first, the final level of o2 uptake was greatly reduced. when 2-oxogluconate was used as a substrate, o2 uptake was immediately halted by pyocin. by determining the amounts of glucose, gluconate, and 2-oxogluconate before and after the reaction and the amount o ... | 1978 | 104980 |
| quantitative assessment of bactericidal activities of beta-lactam antibiotics by agar plate method. | quantitative bactericidal activities of beta-lactam antibiotics were determined by the agar plate method. broth cultures, of which the colony-forming units were counted before the study, were inoculated on antibiotic-containing agar plates, utilizing a 10(-3), 10(-2), or 10(-1) dilution or undiluted culture plated with each 0.001-ml calibrated loop. these plates were incubated at 37 degrees c overnight, and the minimal drug concentration at which no bacterial growth was observed on the plates wa ... | 1978 | 102246 |
| in vitro effect of purified proteases of pseudomonas aeruginosa on rabbit lung macrophages. | | 1978 | 99330 |
| complement-mediated phagocytosis of pseudomonas aeruginosa. | the nature of the opsonic factors in nonimmune human serum for six blood culture isolates of pseudomonas aeruginosa was investigated by measuring uptake of [3h] adenine-labeled bacteria by human pmns. normal human serum, c2- and c4-deficient sera, zymosan-treated serum, and immunoglobulin-deficient sera were used as opsonic sources. heat inactivation of each of these serum sources markedly reduced its opsonic capacity for all pseudomonas strains, suggesting that the serum c system was essential ... | 1978 | 105067 |
| transient defect in the bactericidal capacity of rabbit alveolar macrophages following sublethal shock. | the phagocytic function of alveolar macrophages obtained from rabbits after they were given sublethal shock by tumbling in a modified noble-collip drum was studied. as compared to the phagocytic function of alveolar macrophages from normal rabbits, tumbling produced a statistically significant reduction in in vitro bactericidal capacity for both pseudomonas aeruginosa and escherichia coli. however, there was no alteration in the ingestive phase. the reduced bactericidal capacity was evident with ... | 1979 | 105150 |
| pseudomonas sternotomy wound infection and sternal osteomyelitis. complications after open heart surgery. | pseudomonas aeruginosa sternotomy wound infections occurred in five patients who underwent open heart surgery. the initial isolate in each case was from a mediastinal chest tube routinely cultured on removal. soft-tissue infection developed in two patients, and sternal osteomyelitis developed in three patients. pseudomonas-typing studies showed a correlation between five isolates from chest-tube suction pumps used postoperatively and the wound isolates. analysis of antibiograms of pseudomonas wo ... | 1979 | 105156 |
| [colonization of pseudomonas aeruginosa in the digestive tract of limited-flora mice (author's transl)]. | | 1978 | 105173 |
| [serotype characteristics of clinical strains of pseudomonas aeruginosa]. | a total of 348 p. aeroginosa strains isolated from patients with pulmonary and pleural diseases were studied, and 87% of the test showed the possibility of their serotyping with the use of group-specific agglutinating antisera. serogroups ii, iii, iv were found to be prevalent among the strains isolated from patients with bronchopulmonary pathological states. correlations between definite groups of p. aeroginosa in their sensitivity to antibiotics were established; thus, the cultures belonging t ... | 1979 | 105521 |
| [experience with epidemiologic studies of pyocyaneus infections. i. feasibility of using serotypes and antibiotic resistance as a epidemiologic label for clinical strains]. | the authors carried out serological typing of 98 pseudomonas aeruginosa strains, isolated from patients of burn department of the sklifosovsky first aid institute in january-july, 1974, and of 215 strains obtained from other sources; their sensitivity to 13 antibiotics was determined. pseudomonas aeruginosa cultures isolated from the patients were typed with o-sera of 10 serological types. the presence of several hospital strains of pseudomonas aeruginosa was found by means of serological typing ... | 1978 | 105523 |
| [morphogenesis of pseudomonas aeruginosa colonies]. | | 1978 | 105746 |
| therapeutic experience with netilmicin. | netilmicin, a new aminoglycoside antibiotic, was used to treat 19 patients with urinary tract infection and 5 with systemic infection. the causal organisms were escherichia coli (in 2), klebsiella pneumoniae (in 4), serratia marcescens (in 12) and pseudomonas aeruginosa (in 7); 1 patient was infected with two of these organisms. all the isolates of causal organisms except one of serratia were initially sensitive to netilmicin but many were resistant to other aminoglycosides. sixteen of the urina ... | 1978 | 105797 |
| [evidence for plasmidic localization of "beta-lactamase" gene in a pseudomonas aeruginosa strain, highly resistant to carbenicillin]. | pseudomonas aeruginosa strain hl, isolated in besançon (france), shows a high level of resistance for carbenicillin, as proved in preliminary studies. in this paper, we report the transfer of carbenicillin resistance from pseudomonas aeruginosa hl1 carrying helper plasmids to other strains of the same species. comparative study of beta-lactamase activity in the hl1 strain and in the conjugated strains allows confirmation of the transfer of an r-factor from the hl1 strain: in all these strains, t ... | 1978 | 105822 |
| amikacin and netilmicin resistance in pseudomonas aeruginosa: incidence and transfer. | | 1979 | 105839 |
| time and dose studies of the effect of cobra venom factor on the in vivo immune response in galleria mellonella to pseudomonas aeruginosa. | the induced resistance of wax moth larvae vaccinated with formolized pseudomonas aeruginosa 18 hr prior to challenge with the live organism was significantly inhibited by 0.2 to 1.0 units of cobra venom factor (cvf) per insect given 6 hr after vaccination. approximately 90% inhibition of the protective response was caused by 1.0 units cvf/insect. administration of this dose 6 hr before vaccination, with the vaccine, and 6 hr before challenge had no significant effect. the cvf effect was inhibite ... | 1978 | 99334 |
| separation and characterization of the outer membrane of pseudomonas aeruginosa. | a method is described for the preparation of outer and cytoplasmic membranes of pseudomonas aeruginosa, and the outer membrane proteins characterized. isolated outer and cytoplasmic membranes differed markedly in the content of 2-keto-3-deoxyoctonate (lipopolysaccharide) and phospholipid as well as in the localization of certain enzymes (nadh oxidase, succinate dehydrogenase, d-lactate dehydrogenase, malate dehydrogenase, and phospholipase), and also in the microscopic morphology. the outer memb ... | 1978 | 99443 |
| defective cellular immunity to gram-negative bacteria in cystic fibrosis patients. | in vitro lymphocyte responses to pseudomonas aeruginosa have been found to be impaired in cystic fibrosis patients with advanced clinical disease. the responses to klebsiella pneumoniae, serratia marcescens, and proteus mirabilis were studied in a similar group of cystic fibrosis patients and normal individuals. cystic fibrosis patients found to be unresponsive to pseudomonas were also unresponsive to klebsiella, serratia, and proteus. responsiveness to staphylococcus aureus was not impaired in ... | 1979 | 106011 |
| evaluation of some methods for the laboratory examination of sputum. | methods for the quantitation of leucocytes, squamous epithelial cells, and potential pathogens in sputa are described. microscopic examination showed that 58% of sputa tested (554/957) were purulent or moderately purulent and 48% were moderately or heavily contaminated by squamous epithelial cells. the presence of squamous epithelial cells indicated oropharyngeal contamination. a simple dilution technique was chosen to compare the isolation of potential pathogens from direct cultures and from di ... | 1978 | 99457 |
| sensitivity of pseudomonas aeruginosa envelope mutants to alkylbenzyldimethylammonium chlorides. | a series of stepwise polymyxin-resistant envelope mutants of pseudomonas aeruginosa was used to test the activity of a homologous series (c10-c18) of alkylbenzyldimethylammonium chlorides. a sterilization kinetics procedure in deionized water was devised to avoid amounts quaternary compound above the critical micelle concentration. in all cases, there was a linear relationship between the logarithm of the rate of change of the colony count with time and the logarithm of the homolog concentration ... | 1979 | 106105 |
| [electrophoretic differences in the proteins of the species pseudomonas aeruginosa and pseudomonas denitrificans capable of oxidizing n-alkanes]. | pseudomonas aeruginosa and ps. denitrificans are capable of growing on n-alkanes, but differ in assimilation of higher and lower alkanes. the proteins of their cell-free homogenates were analysed by disc-electrophoresis in a system containing sds. the "preliminarily adapted" strain of ps. aeruginosa did not differ from the "non-adapted" to n-hexane strain, though both differed considerably from ps. denitrificans in the composition of protein spectrum. therefore, disc-electrophoresis of total pro ... | 1978 | 106218 |
| [isolation and general characteristics of a group of pseudomonas aeruginosa temperate phages]. | eighteen temperate phages were isolated from 38 natural strains of pseudomonas aeruginosa. the phages belong to five heteroimmune groups according to the ability of lysogenic variants of the strains pao1 and pat2 to exclude the growth of the superinfecting phage. phages of the ii and v immunity groups are serologically similar; the remaining phages are not inactivated by antisera against phages b26 (ii group) and k1338 (v group). prophages of the ii, iv, and v groups are induced by uv for vegeta ... | 1979 | 106221 |
| antibacterial activity of nitroxoline and sulphamethizole alone and in combination in urinary tract infections. | in vitro studies on the antibacterial activity of nitroxoline and sulphamethizole, alone and in combination, were undertaken and minimal inhibitory concentrations (mics) determined on a range of urinary pathogens. eighty per cent of the strains tested were sensitive to less than or equal to 16 mg/l of nitroxoline, and all strains, including pseudomonas aeruginosa and streptococcus faecalis, were sensitive to less than or equal to 64 mg/l of nitroxoline. no synergism could be demonstrated with su ... | 1978 | 106479 |
| [antibody induced phase variation in pseudomonas aeruginosa (author's transl)]. | to extend the flagella specific h antigenic schema of p. aeruginosa (ansorg, 1978), investigations are carried out to reveal phase variation in this species as in salmonella or arizona. with conventional methods, i.e. examination of single colonies after plating the cultures on standard nutrient agar or the phase reversal techniques of craigie (1931) and gard (1938) by adding homologous anti-oh-serum of different amounts in semisolid agar medium, no change of the flagellar antigens of various p. ... | 1978 | 106580 |
| in vitro and in vivo effect of pc 904, a new broad-spectrum penicillin, on ocular strains of pseudomonas aeruginosa. | the in vitro and in vivo activities of pc 904 were tested against pseudomonas aeruginosa isolated from infected eyes and were compared with carbenicillin and gentamicin. by the microtiter plate method, we tested 30 strains of p. aeruginosa in vitro; pc 904 was very active against 29, but one strain was resistant. in a comparative study, pc 904 was more active than carbenicillin but less active than gentamicin. in vivo tests were conducted on rabbit corneas infected with a strain of p aeruginosa ... | 1978 | 100194 |
| [antibiotic resistance study of clinical strains of pseudomonas aeruginosa]. | the study of 40 clinical strains of ps. aeruginosa isolated from the wound surfaces of the patients showed that all the isolates were resistant to one or several antibiotics. the number of the strains resistant to 5, 4, 3, 2 or 1 drug was 5, 22.5, 25. 30 or 17.5 per cent respectively. fifteen strains carried resistance plasmids capable of conjugative transfer. eleven out of 21 plasmids controlled resistance to chloramphenicol, 7 plasmids controlled resistance to streptomycin and sulfanylamides, ... | 1979 | 106769 |
| pathogenesis of experimental pseudomonas keratitis in the guinea pig: bacteriologic, clinical, and microscopic observations. | uniformly severe corneal infections were produced in guinea pigs by intracorneal injection of about 10 viable pseudomonas aeruginosa. after a brief lag period, multiplication of bacteria was rapid, reaching geometric means of 280,000 after 24 hr and of 5 million after 48 hr. within 8 hr after inoculation, polymorphonuclear leukocytes (pmns) began to infiltrate the anterior two thirds of the stroma. stromal cells adjacent to the injection site became necrotic and appeared to be engulfed by pmns. ... | 1978 | 100468 |
| [hospital infections with pseudomonas aeruginosa. ii. intensive care units (author's transl)]. | 72 newly admitted patients of three surgical intensive care units of the medical school hannover were examined bacteriologically for pseudomonas aeruginosa for a period of 7 months. a total of 810 specimens was examined during therapy. 95 strains of pseudomonas aeruginosa were isolated in 32 of 72 patients, taken either at the time of admission or during the stay of the patients in the hospital. the frequency of contamination increased with the duration of the stay of the patients in the hospita ... | 1978 | 102910 |
| [duodenal bacterial flora in the child during post-surgical stasis]. | the bacterial flora of the small bowel was studied in infants with stasis after surgery. samples were obtained, with radiological assistance, by duodenal aspiration and then immediately analysed by the laboratory in blind tests, both qualitatively and quantitatively. there were two groups of patients: one receiving parenteral nutrition and a second on enteral elementary diet; patients without any gastro-intestinal tract disease formed the control group. pseudomonas aeruginosa and enterobacteria ... | 1978 | 106800 |
| [antibody response to somatic and flagellar antigens of pseudomonas aeruginosa (author's transl)]. | using the indirect fluorescent antibody technique, the production of antiflagellar and antisomatic antibodies in rabbits immunized with different p. aeruginosa strains is measured. after 6 injections of formalinized cultures in graded doses over 3 weeks, the antiflagellar antibodies reach the peak in the 4th to 5th week, followed by a rapid fall. the antisomatic antibodies reacting with native and boiled cells show a plateau-shaped maximum from the 3rd to 5th/6th week and fell slightly up to the ... | 1978 | 102912 |
| persistent bacteriuria in the rat after reflux challenge with pseudomonas aeruginosa. | an experiment based upon reflux of culture suspensions of pseudomonas aeruginosa into the rat kidney was set up to examine possible factors of importance in the development of ascending renal infection. we monitored the persistence of challenge bacteria in the rat urinary tract by quantitative culture of urine collected by spontaneous micturition, despite heavy contamination with commensal microflora. in most animals challenged, pseudomonads gave rise to bacteriuria of only limited duration. dur ... | 1978 | 103062 |
| reversal of succinate-mediated catabolite repression of alkylsulfatase in pseudomonas aeruginosa by 2,4-dinitrophenol and by sodium malonate. | | 1978 | 106946 |
| renal infection in the rat after reflux challenge with pseudomonas aeruginosa. | a rat-pseudomonad reflux model was employed to examine the effect of the size of inoculum on the emergence of renal infection. widely varying degrees of renal disease observed in challenged animals indicated that a number of variables influenced the outcome of the host-parasite interaction. in this regard, animals in which there was a reduction of the colony counts below a threshold figure rarely gave evidence of renal infection when examined at autopsy on the eighth day following reflux. these ... | 1978 | 103063 |
| biosynthesis of peptidoglycan in pseudomonas aeruginosa. 2. mode of action of beta-lactam antibiotics. | the intrinsic effect of various beta-lactam antibiotics on the biosynthesis of peptidoglycan of pseudomonas aeruginosa x-48 was investigated. most of the cephalosporins and penicillins tested already at 0.5 microgram/ml strongly inhibited (a) the incorporation of nascent peptidoglycan into the detergent-insoluble fraction (greater than 75%), (b) the formation of peptide crosslinkages (greater than 60%) and (c) the activity of the dd-carboxypeptidase and partially that of the transpeptidase (appr ... | 1979 | 107030 |
| [automatic rearing system of mice and transmission of pseudomonas aeruginosa]. | mice either excreting or not excreting pseudomonas aeruginosa in feces were maintained in stainless steel mesh cages on an automated rearing apparatus with automatic water-supply nozzles and intermittently flusing metal racks. no evidence of transmission of the organisms from positive mice to negative ones was obtained during at least eight weeks. | 1979 | 107033 |
| effect of active and passive antibacterial immunization on skin allograft survival in rabbits. | the effect of active and passive antibacterial immunization on transplantation immunity was investigated in rabbits. immunization with dead bacterial vaccines prepared from e. coli, proteus mirabilis and pseudmonas aeruginosa had no effect on skin allograft survival, whereas immunization with vaccines prepared from streptococcal and staphylococcal strains shortened allograft survival. similar results were obtained with specific antisera against individual microbial species. skin allografts survi ... | 1979 | 107050 |
| incidence of transferable drug resistance in pseudomonas aeruginosa: transfer of carbenicillin resistance to escherichia coli k12 (f-)--a preliminary report. | | 1979 | 107118 |
| penetration through the gram-negative cell wall: a co-determinant of the efficacy of beta-lactam antibiotics. | resistance of gram-negative bacteria to beta-lactam antibiotics is based mainly on two mechanisms: hydrolysis by beta-lactamases and exclusion of the antibiotics from their target sites in the inner membrane. this article describes the use of pseudomonas aeruginosa k 799/wt and a mutant of this strain (k 799/61) to assess the role of the outer membrane as a permeability barrier to penicillins and cephalosporins. the data confirm the importance of good penetration for a beta-lactam to be active a ... | 1979 | 107128 |
| serotypes and antibiotic susceptibilities of pseudomonas aeruginosa isolates from single sputa of cystic fibrosis patients. | a phenotypic characterization of pseudomonas aeruginosa from single sputum samples of 21 typical cystic fibrosis patients indicated a high frequency of heterogeneity among isolates on the basis of differences in antibiotic resistance, colony morphology, pigmentation, and serotype. two or more isolates with different but stable susceptibilities to carbenicillin, gentamycin, streptomycin, tetracycline, chloramphenicol, and sulfamethoxazole plus trimethoprim were detected in 38% of the sputa. diffe ... | 1979 | 107186 |
| rapid identification of nonfermentative gram-negative rods by the corning n/f system. | a total of 1,298 nonfermentative gram-negative rods were used to evaluate the performance of the individual biochemical tests in the n/f system and to determine whether the observed results included sufficient key reactions for rapid identification. the system included an oxidase test, two screening tubes providing 5 test reactions designed primarily to identify pigment-producing strains of pseudomonas aeruginosa, and a plate providing 12 test reactions designed to identify other nonfermenters. ... | 1979 | 107190 |
| rapid diagnosis of pseudomonas aeruginosa urinary tract infections by radioimmunoassay. | a solid-phase radioimmunoassay designed to detect serotype 6 pseudomonas aeruginosa antigens was evaluated for its ability to rapidly diagnose urinary tract infections. twelve p. aeruginosa serotypes were easily differentiated in the assay from eight other gram-negative bacterial species. during log-phase growth, the assay detected antigens in culture when approximately 10(6) or more serotype 6 p. aeruginosa organisms were present. both cell-associated and solubilized antigens were detected. the ... | 1979 | 107191 |
| [the influence of anti-oep-igg and -igm upon mouse spleen macrophage. phagocytic activity against pseudomonas aeruginosa and oep-coated latex granules (author's transl)]. | | 1978 | 107248 |
| pseudomonas aeruginosa bacteremia: relationship of bacterial enzyme production and pyocine types with clinical prognosis in 100 patients. | strains of pseudomonas aeruginosa were studied from 100 patients with bacteremia. in vitro quantitation of extracellular enzymes (lecithinase, protease, and elastase) and pyocine typing of these isolates were performed. no significant difference was found in the quantity of the enzymes produced or in the pyocine types by isolates obtained from patients dying from bacteremia or surviving this serious infection. quantitation of the extracellular enzymes and pyocine types of blood isolates were con ... | 1979 | 107256 |
| the instability of mucoid pseudomonas aeruginosa: fluctuation test and improved stability of the mucoid form in shaken culture. | | 1979 | 107270 |
| effect of two cancer chemotherapeutic agents on the antibacterial activity of three antimicrobial agents. | cancer chemotherapeutic agents and antibacterial antibiotics are often given concomitantly. daunorubicin, cytosine arabinoside, and three antibiotics (gentamicin, amikacin, and ticarcillin) were tested individually and in combinations to determine their antimicrobial activity against pseudomonas aeruginosa, klebsiella pneumoniae, and escherichia coli. these cytotoxic agents are commonly employed in the therapy of acute nonlymphocytic leukemia for remission induction therapy, and these antimicrob ... | 1978 | 103494 |
| partial purification of heat-labile hemolysin from pseudomonas aeruginosa. | | 1978 | 107353 |
| [studies of the incidence of healthy carriers of "pathogenic strains" or potentially pathogenic strains in the hospital environment]. | an investigation has been carried out on healthy carriers of "positive staphylococcus coagulase" and "occasional pathogenic" strains among kitchen, canteen and dispensary staff in the ivth geriatric division and first aid clinic of the bologna "m. malpighi" hospital. all stains of "positive staphylococcus coagulase" and "occasional pathogens" considered (pseudomonas aeruginosa and citrobacter) were put through antibiotic and chemotherapeutic sensitivity tests. the results are reported in detail. | 1979 | 107491 |
| [pubic osteitis caused by pseudomonas aeruginosa]. | | 1978 | 107573 |
| frequency of f116-mediated transduction of pseudomonas aeruginosa in a freshwater environment. | transduction of pseudomonas aeruginosa streptomycin resistance by a generalized transducing phage, f116, was shown to occur during a 10-day incubation in a flow-through environmental test chamber suspended in a freshwater reservoir. mean f116 transduction frequencies ranged from 1.4 x 10(-5) to 8.3 x 10(-2) transductants per recipient during the in situ incubation. these transduction frequencies were comparable to transduction frequencies determined in preliminary laboratory transduction experim ... | 1978 | 103503 |
| experimental urinary tract infection with pseudomonas aeruginosa in mice. | urinary tract infection with pseudomonas aeruginosa was induced in mice by transurethral inoculation of the organism into the bladder, followed by urethral obstruction for 6 h. the infection was mostly localized in the urinary organs. p. aeruginosa p9 was selected as the challenge organism from 10 laboratory strains of p. aeruginosa. after the inoculation of 10(7) colony-forming units of p. aeruginosa p9, transient bacteremia was observed in some of the mice from 6 h to 1 day after the inoculati ... | 1978 | 103833 |