| quality control and stability studies with the monoclonal antibody, trastuzumab: application of 1d- vs. 2d-gel electrophoresis. | recombinant monoclonal antibodies (rmabs) are medicinal products obtained by rdna technology. consequently, like other biopharmaceuticals, they require the extensive and rigorous characterization of the quality attributes, such as identity, structural integrity, purity and stability. the aim of this work was to study the suitability of gel electrophoresis for the assessment of charge heterogeneity, post-translational modifications and the stability of the therapeutic, recombinant monoclonal anti ... | 2014 | 24739811 |
| multiresistant elizabethkingia meningoseptica infections in tertiary care. | | 2014 | 26288498 |
| multiresistant elizabethkingia meningoseptica infections in tertiary care. | | 2014 | 26288498 |
| new β-lactamase inhibitors: a therapeutic renaissance in an mdr world. | as the incidence of gram-negative bacterial infections for which few effective treatments remain increases, so does the contribution of drug-hydrolyzing β-lactamase enzymes to this serious clinical problem. this review highlights recent advances in β-lactamase inhibitors and focuses on agents with novel mechanisms of action against a wide range of enzymes. to this end, we review the β-lactamase inhibitors currently in clinical trials, select agents still in preclinical development, and older the ... | 2014 | 24379206 |
| impact of myeloperoxidase-ldl interactions on enzyme activity and subsequent posttranslational oxidative modifications of apob-100. | oxidation of ldl by the myeloperoxidase (mpo)-h2o2-chloride system is a key event in the development of atherosclerosis. the present study aimed at investigating the interaction of mpo with native and modified ldl and at revealing posttranslational modifications on apob-100 (the unique apolipoprotein of ldl) in vitro and in vivo. using amperometry, we demonstrate that mpo activity increases up to 90% when it is adsorbed at the surface of ldl. this phenomenon is apparently reflected by local stru ... | 2014 | 24534704 |
| current and emerging therapy for celiac disease. | at present, strict and lifelong gluten-free diet is the only effective treatment for celiac disease. even small amounts of gluten (50 mg/day) can be immunogenic; therefore all food and food items and drugs that contain gluten and its derivatives must be eliminated completely from the diet. while prescribing gluten-free diet is easy; the key to the success is the dietary counseling by a nutrition specialist and maintenance of adherence to gfd by the patient. in recent times, a number of targets t ... | 2014 | 25705619 |
| endoe from enterococcus faecalis hydrolyzes the glycans of the biofilm inhibiting protein lactoferrin and mediates growth. | glycosidases are widespread among bacteria. the opportunistic human pathogen enterococcus faecalis encodes several putative glycosidases but little is known about their functions. the identified endo-β-n-acetylglucosaminidase endoe has activity on the n-linked glycans of the human immunoglobulin g (igg). in this report we identified the human glycoprotein lactoferrin (hlf) as a new substrate for endoe. hydrolysis of the n-glycans from hlf was investigated using lectin blot, uhplc and mass spectr ... | 2014 | 24608122 |
| bacteriology and drug susceptibility analysis of pus from patients with severe intra-abdominal infection induced by abdominal trauma. | the aim of the present study was to retrospectively analyze the bacteriology and drug susceptibility of pus flora from abdominal trauma patients with severe intra-abdominal infection (siai). a total of 41 patients with siai induced by abdominal trauma were enrolled in the study, from which 123 abdominal pus samples were obtained. the results from laboratory microbiology and drug sensitivity were subjected to susceptibility analysis using whonet software. a total of 297 strains were isolated in w ... | 2014 | 24940451 |
| exploring anopheles gut bacteria for plasmodium blocking activity. | malaria parasite transmission requires the successful development of plasmodium gametocytes into flagellated microgametes upon mosquito blood ingestion, and the subsequent fertilization of microgametes and macrogametes for the development of motile zygotes, called ookinetes, which invade and transverse the anopheles vector mosquito midgut at around 18-36 h after blood ingestion. within the mosquito midgut, the malaria parasite has to withstand the mosquito's innate immune response and the detrim ... | 2014 | 24428613 |
| neonatal meningitis by multidrug resistant elizabethkingia meningosepticum identified by 16s ribosomal rna gene sequencing. | clinical and microbiological profile of 9 neonates with meningitis by elizabethkingia meningosepticum identified by 16s ribosomal gene sequencing was studied. all the clinical isolates were resistant to cephalosporins, aminoglycosides, trimethoprim-sulfamethoxazole, β -lactam combinations, carbapenems and only one isolate was susceptible to ciprofloxacin. all the isolates were susceptible to vancomycin. six of nine neonates died even after using vancomycin, based on susceptibility results. e. me ... | 2014 | 24678326 |
| structural analysis of the endogenous glycoallergen hev b 2 (endo-β-1,3-glucanase) from hevea brasiliensis and its recognition by human basophils. | endogenous glycosylated hev b 2 (endo-β-1,3-glucanase) from hevea brasiliensis is an important latex allergen that is recognized by ige antibodies from patients who suffer from latex allergy. the carbohydrate moieties of hev b 2 constitute a potentially important ige-binding epitope that could be responsible for its cross-reactivity. here, the structure of the endogenous isoform ii of hev b 2 that exhibits three post-translational modifications, including an n-terminal pyroglutamate and two glyc ... | 2014 | 24531467 |
| filter-aided n-glycan separation (fangs): a convenient sample preparation method for mass spectrometric n-glycan profiling. | we have developed a simple method for the release and isolation of glycoprotein n-glycans from whole-cell lysates using less than a million cells, for subsequent implementation with mass spectrometric analysis. cellular protein extracts prepared using sds solubilization were sequentially treated in a membrane filter device to ultimately release glycans enzymatically using pngase f in the volatile buffer ammonium bicarbonate. the released glycans are recovered in the filtrate following centrifuga ... | 2014 | 24450425 |
| low-molecular-mass penicillin binding protein 6b (dacd) is required for efficient gob-18 metallo-β-lactamase biogenesis in salmonella enterica and escherichia coli. | metallo-β-lactamases (mbls) are zn(2+)-containing secretory enzymes of clinical relevance, whose final folding and metal ion assembly steps in gram-negative bacteria occur after secretion of the apo form to the periplasmic space. in the search of periplasmic factors assisting mbl biogenesis, we found that dacd null (δdacd) mutants of salmonella enterica and escherichia coli expressing the pre-gob-18 mbl gene from plasmids showed significantly reduced resistance to cefotaxime and concomitant lowe ... | 2014 | 24145538 |
| structural basis of metallo-β-lactamase inhibition by captopril stereoisomers. | β-lactams are the most successful antibacterials, but their effectiveness is threatened by resistance, most importantly by production of serine- and metallo-β-lactamases (mbls). mbls are of increasing concern because they catalyze the hydrolysis of almost all β-lactam antibiotics, including recent-generation carbapenems. clinically useful serine-β-lactamase inhibitors have been developed, but such inhibitors are not available for mbls. l-captopril, which is used to treat hypertension via angiote ... | 2015 | 26482303 |
| structural basis of metallo-β-lactamase inhibition by captopril stereoisomers. | β-lactams are the most successful antibacterials, but their effectiveness is threatened by resistance, most importantly by production of serine- and metallo-β-lactamases (mbls). mbls are of increasing concern because they catalyze the hydrolysis of almost all β-lactam antibiotics, including recent-generation carbapenems. clinically useful serine-β-lactamase inhibitors have been developed, but such inhibitors are not available for mbls. l-captopril, which is used to treat hypertension via angiote ... | 2015 | 26482303 |
| the soil microbiota harbors a diversity of carbapenem-hydrolyzing β-lactamases of potential clinical relevance. | the origin of carbapenem-hydrolyzing metallo-β-lactamases (mbls) acquired by clinical bacteria is largely unknown. we investigated the frequency, host range, diversity, and functionality of mbls in the soil microbiota. twenty-five soil samples of different types and geographical origins were analyzed by antimicrobial selective culture, followed by phenotypic testing and expression of mbl-encoding genes in escherichia coli, and whole-genome sequencing of mbl-producing strains was performed. carba ... | 2015 | 26482314 |
| the soil microbiota harbors a diversity of carbapenem-hydrolyzing β-lactamases of potential clinical relevance. | the origin of carbapenem-hydrolyzing metallo-β-lactamases (mbls) acquired by clinical bacteria is largely unknown. we investigated the frequency, host range, diversity, and functionality of mbls in the soil microbiota. twenty-five soil samples of different types and geographical origins were analyzed by antimicrobial selective culture, followed by phenotypic testing and expression of mbl-encoding genes in escherichia coli, and whole-genome sequencing of mbl-producing strains was performed. carba ... | 2015 | 26482314 |
| structure-function characterization reveals new catalytic diversity in the galactose oxidase and glyoxal oxidase family. | alcohol oxidases, including carbohydrate oxidases, have a long history of research that has generated fundamental biological understanding and biotechnological applications. despite a long history of study, the galactose 6-oxidase/glyoxal oxidase family of mononuclear copper-radical oxidases, auxiliary activity family 5 (aa5), is currently represented by only very few characterized members. here we report the recombinant production and detailed structure-function analyses of two homologues from ... | 2015 | 26680532 |
| performances and reliability of bruker microflex lt and vitek ms maldi-tof mass spectrometry systems for the identification of clinical microorganisms. | in clinical microbiology laboratories, routine microbial identification is mostly performed using culture based methodologies requiring 24 to 72 hours from culturing to identification. matrix assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) technology has been established as a cost effective, reliable, and faster alternative identification platform. in this study, we evaluated the reliability of the two available maldi-tof ms systems for their routine clinical ... | 2015 | 26793718 |
| lysosome associated membrane proteins maintain pancreatic acinar cell homeostasis: lamp-2 deficient mice develop pancreatitis. | the pathogenic mechanism of pancreatitis is poorly understood. recent evidence implicates defective autophagy in pancreatitis responses; however, the pathways mediating impaired autophagy in pancreas remain largely unknown. here, we investigate the role of lysosome associated membrane proteins (lamps) in pancreatitis. | 2015 | 26693174 |
| noncontiguous finished genome sequence and description of weeksella massiliensis sp. nov. | strain ff8(t) (= csur p860 = dsm 28259) was isolated in dakar, senegal, from the urine of a 65-year-old man with acute cystitis. this strain shows a similarity of sequence of 16s rrna of 98.38% with weeksella virosa, and its genbank accession numbers are hg931340 and ccmh00000000. matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis had a poor score, ranging from 1.32 to 1.56, that did not allow identification of the bacterium. using a polyphasic study made of ph ... | 2015 | 26649182 |
| empirical antibiotic therapy for pneumonia in intensive care units: a multicentre, retrospective analysis of potentially pathogenic microorganisms identified by endotracheal aspirates cultures. | the purpose of this investigation was to explore the presumed relationship between the days of hospitalisation and microorganisms identified by endotracheal aspirate cultures in relation to adequate empirical treatment strategies of pneumonia in the intensive care unit (icu). all potentially pathogenic microorganisms identified by (surveillance) cultures of endotracheal aspirates obtained in the icus of two dutch teaching hospitals in 2007 and 2012 were retrospectively collected and analysed. an ... | 2015 | 26385348 |
| overcoming differences: the catalytic mechanism of metallo-β-lactamases. | metallo-β-lactamases are the latest resistance mechanism of pathogenic and opportunistic bacteria against carbapenems, considered as last resort drugs. the worldwide spread of genes coding for these enzymes, together with the lack of a clinically useful inhibitor, have raised a sign of alarm. inhibitor design has been mostly impeded by the structural diversity of these enzymes. here we provide a critical review of mechanistic studies of the three known subclasses of metallo-β-lactamases, analyze ... | 2015 | 26297824 |
| host response to the lung microbiome in chronic obstructive pulmonary disease. | the relatively sparse but diverse microbiome in human lungs may become less diverse in chronic obstructive pulmonary disease (copd). this article examines the relationship of this microbiome to emphysematous tissue destruction, number of terminal bronchioles, infiltrating inflammatory cells, and host gene expression. | 2015 | 25945594 |
| complementary lc-ms/ms-based n-glycan, n-glycopeptide, and intact n-glycoprotein profiling reveals unconventional asn71-glycosylation of human neutrophil cathepsin g. | neutrophil cathepsin g (ncg) is a central serine protease in the human innate immune system, but the importance of its n-glycosylation remains largely undescribed. to facilitate such investigations, we here use complementary lc-ms/ms-based n-glycan, n-glycopeptide, and intact glycoprotein profiling to accurately establish the micro- and macro-heterogeneity of ncg from healthy individuals. the fully occupied asn71 carried unconventional n-glycosylation consisting of truncated chitobiose core (glc ... | 2015 | 26274980 |
| draft genome sequence of strain atcc 33958, reported to be elizabethkingia miricola. | we report the draft genome of elizabethkingia strain atcc 33958, which has been classified as elizabethkingia miricola. similar to other elizabethkingia species, the atcc 33958 draft genome contains numerous β-lactamase genes. atcc 33958 also harbors a urease gene cluster which supports classification as e. miricola. | 2015 | 26205869 |
| structure-based mechanism of oleate hydratase from elizabethkingia meningoseptica. | hydratases provide access to secondary and tertiary alcohols by regio- and/or stereospecifically adding water to carbon-carbon double bonds. thereby, hydroxy groups are introduced without the need for costly cofactor recycling, and that makes this approach highly interesting on an industrial scale. here we present the first crystal structure of a recombinant oleate hydratase originating from elizabethkingia meningoseptica in the presence of flavin adenine dinucleotide (fad). a structure-based mu ... | 2015 | 26077980 |
| structure and functional properties of norrin mimic wnt for signalling with frizzled4, lrp5/6, and proteoglycan. | wnt signalling regulates multiple processes including angiogenesis, inflammation, and tumorigenesis. norrin (norrie disease protein) is a cystine-knot like growth factor. although unrelated to wnt, norrin activates the wnt/β-catenin pathway. signal complex formation involves frizzled4 (fz4), low-density lipoprotein receptor related protein 5/6 (lrp5/6), tetraspanin-12 and glycosaminoglycans (gags). here, we report crystallographic and small-angle x-ray scattering analyses of norrin in complex wi ... | 2015 | 26158506 |
| mutations in plasmalemma vesicle associated protein result in sieving protein-losing enteropathy characterized by hypoproteinemia, hypoalbuminemia, and hypertriglyceridemia. | severe intestinal diseases observed in very young children are often the result of monogenic defects. we used whole exome sequencing (wes) to examine the genetic cause in a patient with a distinct severe form of protein losing enteropathy (ple) characterized by hypoproteinemia, hypoalbuminemia, and hypertriglyceridemia. | 2015 | 26207260 |
| complete genome sequence of elizabethkingia meningoseptica, isolated from a t-cell non-hodgkin's lymphoma patient. | an elizabethkingia meningoseptica infection was detected at the end stage of a patient with t-cell non-hodgkin's lymphoma. the complete genome of this isolated strain, fms-007, was generated in one contig with a total size of 3,938,967 bp. a preliminary screening indicated that the genome contains drug resistance genes to aminoglycosides and β-lactams. a clustered regularly interspaced short palindromic repeats (crispr)/crispr-associated proteins (crispr/cas) system with 16 direct repeats and 15 ... | 2015 | 26112786 |
| characterization of the glycosylation site of human psa prompted by missense mutation using lc-ms/ms. | prostate specific antigen (psa) is currently used as a diagnostic biomarker for prostate cancer. it is a glycoprotein possessing a single glycosylation site at n69. during our previous study of psa n69 glycosylation, additional glycopeptides were observed in the psa sample that were not previously reported and did not match glycopeptides of impure glycoproteins existing in the sample. this extra glycosylation site of psa is associated with a mutation in klk3 genes. among single nucleotide polymo ... | 2015 | 26022737 |
| paratransgenesis: a promising new strategy for mosquito vector control. | the three main mosquito genera, anopheles, aedes and culex, transmit respectively malaria, dengue and lymphatic filariasis. current mosquito control strategies have proved unsuccessful, and there still is a substantial number of morbidity and mortality from these diseases. genetic control methods have now arisen as promising alternative strategies, based on two approaches: the replacement of a vector population by disease-refractory mosquitoes and the release of mosquitoes carrying a lethal gene ... | 2015 | 26104575 |
| comparative analysis of the glycosylation profiles of membrane-anchored hiv-1 envelope glycoprotein trimers and soluble gp140. | the human immunodeficiency virus type 1 (hiv-1) envelope glycoprotein (env) trimer, which consists of the gp120 and gp41 subunits, is the focus of multiple strategies for vaccine development. extensive env glycosylation provides hiv-1 with protection from the immune system, yet the glycans are also essential components of binding epitopes for numerous broadly neutralizing antibodies. recent studies have shown that when env is isolated from virions, its glycosylation profile differs significantly ... | 2015 | 26018173 |
| complete genome sequence and transcriptomic analysis of the novel pathogen elizabethkingia anophelis in response to oxidative stress. | elizabethkingia anophelis is an emerging pathogen that can cause life-threatening infections in neonates, severely immunocompromised and postoperative patients. the lack of genomic information on e. anophelis hinders our understanding of its mechanisms of pathogenesis. here, we report the first complete genome sequence of e. anophelis nuhp1 and assess its response to oxidative stress. elizabethkingia anophelis nuhp1 has a circular genome of 4,369,828 base pairs and 4,141 predicted coding sequenc ... | 2015 | 26019164 |
| pneumonia in the neutropenic cancer patient. | pneumonia is the leading cause of death among neutropenic cancer patients, particularly those with acute leukaemia. even with empiric therapy, case fatality rates of neutropenic pneumonias remain unacceptably high. however, recent advances in the management of neutropenic pneumonia offer hope for improved outcomes in the cancer setting. this review summarizes recent literature regarding the clinical presentation, microbiologic trends, diagnostic advances and therapeutic recommendations for cance ... | 2015 | 25784246 |
| leprous lesion presents enrichment of opportunistic pathogenic bacteria. | leprosy is a chronic infectious disease that remains a major challenge to public health in endemic countries. increasing evidence has highlighted the importance of microbiota for human general health and, as such, the study of skin microbiota is of interest. but while studies are continuously revealing the complexity of human skin microbiota, the microbiota of leprous cutaneous lesions has not yet been characterized. here we used sanger and massively parallel small sub-unit rrna (ssu) rrna gene ... | 2015 | 25918684 |
| breaking the allergic response by disrupting antibody glycosylation. | | 2015 | 25847971 |
| self-cleavage of the pseudomonas aeruginosa cell-surface signaling anti-sigma factor foxr occurs through an n-o acyl rearrangement. | the fox system of pseudomonas aeruginosa is a cell-surface signaling (css) pathway employed by the bacterium to sense and respond to the presence of the heterologous siderophore ferrioxamine in the environment. this regulatory pathway controls the transcription of the foxa ferrioxamine receptor gene through the extracytoplasmic function sigma factor σ(foxi). in the absence of ferrioxamine, the activity of σ(foxi) is inhibited by the transmembrane anti-sigma factor foxr. upon binding of ferrioxam ... | 2015 | 25809487 |
| n-linked glycan profiling in neuroblastoma cell lines. | although mycn amplification has been associated with aggressive neuroblastoma, the molecular mechanisms that differentiate low-risk, mycn-nonamplified neuroblastoma from high-risk, mycn-amplified disease are largely unknown. genomic and proteomic studies have been limited in discerning differences in signaling pathways that account for this heterogeneity. n-linked glycosylation is a common protein modification resulting from the attachment of sugars to protein residues and is important in cell s ... | 2015 | 25730103 |
| multidomain human peroxidasin 1 is a highly glycosylated and stable homotrimeric high spin ferric peroxidase. | human peroxidasin 1 (hspxd01) is a multidomain heme peroxidase that uses bromide as a cofactor for the formation of sulfilimine cross-links. the latter confers critical structural reinforcement to collagen iv scaffolds. here, hspxd01 and various truncated variants lacking nonenzymatic domains were recombinantly expressed in hek cell lines. the n-glycosylation site occupancy and disulfide pattern, the oligomeric structure, and unfolding pathway are reported. the homotrimeric iron protein contains ... | 2015 | 25713063 |
| whole-genome sequence of chryseobacterium oranimense, a colistin-resistant bacterium isolated from a cystic fibrosis patient in france. | for the first time, we report the whole-genome sequence analysis of chryseobacterium oranimense g311, a multidrug-resistant bacterium, from a cystic fibrosis patient in france, including resistance to colistin. whole-genome sequencing of c. oranimense g311 was performed using ion torrent pgm, and rast, the embl-ebi server, and the antibiotic resistance gene-annotation (arg-annot) database were used for annotation of all genes, including antibiotic resistance (ar) genes. general features of the c ... | 2015 | 25583710 |
| human neutrophils secrete bioactive paucimannosidic proteins from azurophilic granules into pathogen-infected sputum. | unlike plants and invertebrates, mammals reportedly lack proteins displaying asparagine (n)-linked paucimannosylation (mannose(1-3)fucose(0-1)n-acetylglucosamine(2)asn). enabled by technology advancements in system-wide biomolecular characterization, we document that protein paucimannosylation is a significant host-derived molecular signature of neutrophil-rich sputum from pathogen-infected human lungs and is negligible in pathogen-free sputum. five types of paucimannosidic n-glycans were carrie ... | 2015 | 25645918 |
| evidence for elizabethkingia anophelis transmission from mother to infant, hong kong. | elizabethkingia anophelis, recently discovered from mosquito gut, is an emerging bacterium associated with neonatal meningitis and nosocomial outbreaks. however, its transmission route remains unknown. we use rapid genome sequencing to investigate 3 cases of e. anophelis sepsis involving 2 neonates who had meningitis and 1 neonate's mother who had chorioamnionitis. comparative genomics revealed evidence for perinatal vertical transmission from a mother to her neonate; the 2 isolates from these p ... | 2015 | 25625669 |
| identification and characterization of a novel prokaryotic peptide: n-glycosidase from elizabethkingia meningoseptica. | peptide:n-glycosidase (pngase) f, the first pngase identified in prokaryotic cells, catalyzes the removal of intact asparagine-linked oligosaccharide chains from glycoproteins and/or glycopeptides. since its discovery in 1984, pngase f has remained as the sole prokaryotic pngase. recently, a novel gene encoding a protein with a predicted pngase domain was identified from a clinical isolate of elizabethkingia meningoseptica. in this study, the candidate protein was expressed in vitro and was subj ... | 2015 | 25614628 |
| bacteremia due to elizabethkingia meningoseptica. | elizabethkingia meningoseptica is a nonfermentative gram-negative bacillus that is ubiquitously found in hospital environments and as such, it has been associated with various nosocomial infections. immunocompromised individuals are particularly at increased risk for developing severe infections due to e. meningoseptica, including bacteremia. e. meningoseptica is resistant to multiple antimicrobials commonly used for gram-negative bacteria and conventional empirical antimicrobials targeting thos ... | 2015 | 26793448 |
| automated annotation and quantitation of glycans by liquid chromatography/electrospray ionization mass spectrometric analysis using the multiglycan-esi computational tool. | liquid chromatography/mass spectrometry (lc/ms) is currently considered to be a conventional glycomics analysis strategy due to the high sensitivity and ability to handle complex biological samples. interpretation of lc/ms data is a major bottleneck in high-throughput glycomics lc/ms-based analysis. the complexity of lc/ms data associated with biological samples prompts the needs to develop computational tools capable of facilitating automated data annotation and quantitation. | 2015 | 25462374 |
| septin dynamics are essential for exocytosis. | septins are a family of 14 cytoskeletal proteins that dynamically form hetero-oligomers and organize membrane microdomains for protein complexes. the previously reported interactions with snare proteins suggested the involvement of septins in exocytosis. however, the contradictory results of up- or down-regulation of septin-5 in various cells and mouse models or septin-4 in mice suggested either an inhibitory or a stimulatory role for these septins in exocytosis. the involvement of the ubiquitou ... | 2015 | 25575596 |
| discovery, primary, and crystal structures and capacitation-related properties of a prostate-derived heparin-binding protein wga16 from boar sperm. | mammalian sperm acquire fertility through a functional maturation process called capacitation, where sperm membrane molecules are drastically remodeled. in this study, we found that a wheat germ agglutinin (wga)-reactive protein on lipid rafts, named wga16, is removed from the sperm surface on capacitation. wga16 is a prostate-derived seminal plasma protein that has never been reported and is deposited on the sperm surface in the male reproductive tract. based on protein and cdna sequences for p ... | 2015 | 25568322 |
| recombinant α-nacetylgalactosaminidase from marine bacterium-modifying a erythrocyte antigens. | a plasmid based on pet-40b was constructed to synthesize recombinant α-n-acetylgalactosaminidase of the marine bacterium arenibacter latericius kmm 426t (α-alnagal) in escherichia coli cells. the yield of α-al- nagal attains 10 mg/ml with activity of 49.7 ± 1.3 u at 16°c, concentration of inductor 2 mm, and cultivation for 12 h. techniques such as anion exchange, metal affinity and gel filtration chromatography to purify α-alnagal were applied. α-alnagal is a homodimer with a molecular weight of ... | 2015 | 25927009 |
| elizabethkingia meningoseptica outbreak in intensive care unit. | | 2015 | 25722622 |
| correction for pradeep et al., draft genome sequence of elizabethkingia anopheles, isolated from a postoperative endophthalmitis patient. | | 2016 | 27932658 |
| taxonogenomics and description of vaginella massiliensis gen. nov., sp. nov., strain marseille p2517(t), a new bacterial genus isolated from the human vagina. | an obligate aerobic, gram-negative, nonmotile and nonsporulating rod designated marseille p2517 was isolated from the vaginal flora. we describe its features, annotate the genome and compare it to the closest species. the 16s rrna analysis shows 93.03% sequence similarity with weeksella virosa, the phylogenetically closest species. its genome is 2 434 475 bp long and presents 38.16% g+c. on the basis of these data, it can be considered as a new genus in the flavobacteriaceae family, for which we ... | 2016 | 28053707 |
| taxonogenomics and description of vaginella massiliensis gen. nov., sp. nov., strain marseille p2517(t), a new bacterial genus isolated from the human vagina. | an obligate aerobic, gram-negative, nonmotile and nonsporulating rod designated marseille p2517 was isolated from the vaginal flora. we describe its features, annotate the genome and compare it to the closest species. the 16s rrna analysis shows 93.03% sequence similarity with weeksella virosa, the phylogenetically closest species. its genome is 2 434 475 bp long and presents 38.16% g+c. on the basis of these data, it can be considered as a new genus in the flavobacteriaceae family, for which we ... | 2016 | 28053707 |
| cp40 from corynebacterium pseudotuberculosis is an endo-β-n-acetylglucosaminidase. | c. pseudotuberculosis is an important animal pathogen that causes substantial economical loss in sheep and goat farming. zoonotic infections in humans are rare, but when they occur they are often severe and difficult to treat. one of the most studied proteins from this bacterium, the secreted protein cp40 is being developed as a promising vaccine candidate and has been characterized as a serine protease. in this study we have investigated if cp40 is an endoglycosidase rather than a protease. | 2016 | 27821068 |
| genome sequence of elizabethkingia meningoseptica em1, isolated from a patient with a bloodstream infection. | elizabethkingia meningoseptica em1 was isolated from a whole-blood sample from a female patient. the draft genome sequence of em1 contains 4,038,467 bp, with a g+c content of 36.37%. a preliminary genome analysis showed that em1 contains genes conferring resistance to β-lactams. the bacterium has hemolysin genes and a set of genes involved in heme uptake and heme utilization, showing its potential to cause bloodstream infections. a clustered regularly interspaced short palindromic repeat (crispr ... | 2016 | 27789634 |
| protein n-glycosylation and n-glycan trimming are required for postembryonic development of the pest beetle tribolium castaneum. | in holometabolous insects the transition from larva to adult requires a complete body reorganization and relies on n-glycosylated proteins. n-glycosylation is an important posttranslational modification that influences protein activity but its impact on the metamorphosis has not been studied yet. here we used the red flour beetle, tribolium castaneum, to perform a first comprehensive study on the involvement of the protein n-glycosylation pathway in metamorphosis. the transcript levels for genes ... | 2016 | 27731363 |
| the structural basis for cd36 binding by the malaria parasite. | cd36 is a scavenger receptor involved in fatty acid metabolism, innate immunity and angiogenesis. it interacts with lipoprotein particles and facilitates uptake of long chain fatty acids. it is also the most common target of the pfemp1 proteins of the malaria parasite, plasmodium falciparum, tethering parasite-infected erythrocytes to endothelial receptors. this prevents their destruction by splenic clearance and allows increased parasitaemia. here we describe the structure of cd36 in complex wi ... | 2016 | 27667267 |
| persistent infection by wolbachia walbb has no effect on composition of the gut microbiota in adult female anopheles stephensi. | the bacteria in the midgut of anopheles stephensi adult females from laboratory colonies were studied by sequencing the v4 region of 16s rrna genes, with respect to three experimental factors: stable or cured wolbachia infection; sugar or blood diet; and age. proteobacteria and bacteroidetes dominated the community [>90% of operational taxonomic units (otus)]; most taxa were in the classes flavobacteriia, gammaproteobacteria, and alphaproteobacteria, and were assigned to elizabethkingia (46.9%), ... | 2016 | 27708633 |
| carbapenem breakpoints for acinetobacter baumannii group: supporting clinical outcome data from patients with bacteremia. | the carbapenem breakpoints set by different organizations for acinetobacter are discordant, but supporting clinical data are lacking. this study aimed to provide the first clinical outcome data to support the carbapenem breakpoints for acinetobacter baumannii (ab) group in patients with bacteremia. this study included 117 adults who received carbapenems for treatment of ab group bacteremia in taipei veterans general hospital over an 8-year period. we analyzed 30-day mortality rates among patient ... | 2016 | 27644087 |
| functional integration of the circulatory, immune, and respiratory systems in mosquito larvae: pathogen killing in the hemocyte-rich tracheal tufts. | as both larvae and adults, mosquitoes encounter a barrage of immune insults, ranging from microbe-rich communities in larval habitats to ingested blood-borne pathogens in adult blood meals. given that mosquito adults have evolved an efficient means of eliminating infections in their hemocoel (body cavity) via the coordinated action of their immune and circulatory systems, the goal of the present study was to determine whether such functional integration is also present in larvae. | 2016 | 27643786 |
| applicability of an in-house saponin-based extraction method in bruker biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry system for identification of bacterial and fungal species in positively flagged blood cultures. | we used an in-house saponin-based extraction method to evaluate the performance of the bruker biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof/ms) system for the identification of bacteria and fungi in 405 positively flagged blood culture bottles. results obtained from maldi-tof/ms were compared with those obtained using conventional phenotypic identification methods. of the 405 positively flagged blood culture bottles, 365 showed monomicrobal grow ... | 2016 | 27695442 |
| isolation, antimicrobial susceptibility profile and detection of sul1, blatem, and blashv in amoxicillin-clavulanate-resistant bacteria isolated from retail sausages in kampar, malaysia. | due to the overuse of antibiotics in livestock as a growth-promoting agent, the emergence of multi-antibiotic resistant bacteria is becoming a concern. | 2016 | 27942365 |
| use of maldi-tof ms and culturomics to identify mosquitoes and their midgut microbiota. | mosquitoes transmit a wide range of human parasitic and viral diseases. in recent years, new techniques such as maldi-tof ms have been developed to identify mosquitoes at the species level, which is key for entomological surveys. additionally, there is increasing interest in the mosquito microbiota and its role in vector capacity. | 2016 | 27613238 |
| elevated plasma matrix metalloproteinase-9 and its correlations with severity of disease in patients with ventilator-associated pneumonia. | ventilator-associated pneumonia (vap) increases patient mortality and medical expenditure, and a real-time and reliable method for the rapid diagnosis of vap may help reduce fatal complications. matrix metalloproteinases-9 (mmp-9) is considered significant in the pathogenesis of lung inflammation and infection. therefore, we examined its relationship with the clinical course of vap. this retrospective observational study recruited 30 healthy volunteers, 12 patients who used mechanical ventilatio ... | 2016 | 27499696 |
| a complete profile on blind-your-eye mangrove excoecaria agallocha l. (euphorbiaceae): ethnobotany, phytochemistry, and pharmacological aspects. | traditional system of medicine consists of large number of plants with various medicinal and pharmacological importances. this article provides a comprehensive review of the complete profile of an important mangrove plant excoecaria agallocha l. (euphorbiaceae) and elaborately describing the ethnobotany, phytochemistry, and pharmacological properties. it is used traditionally in the treatment of various diseases such as epilepsy, ulcers, leprosy, rheumatism, and paralysis. the latex obtained fro ... | 2016 | 28082796 |
| complete genome sequences of four strains from the 2015-2016 elizabethkingia anophelis outbreak. | the complete circularized genome sequences of selected specimens from the largest known elizabethkingia anophelis outbreak to date are described here. genomic rearrangements observed among the outbreak strains are discussed. | 2016 | 27313304 |
| cross-class metallo-β-lactamase inhibition by bisthiazolidines reveals multiple binding modes. | metallo-β-lactamases (mbls) hydrolyze almost all β-lactam antibiotics and are unaffected by clinically available β-lactamase inhibitors (βlis). active-site architecture divides mbls into three classes (b1, b2, and b3), complicating development of βlis effective against all enzymes. bisthiazolidines (btzs) are carboxylate-containing, bicyclic compounds, considered as penicillin analogs with an additional free thiol. here, we show both l- and d-btz enantiomers are micromolar competitive βlis of al ... | 2016 | 27303030 |
| antibacterial activity of blue light against nosocomial wound pathogens growing planktonically and as mature biofilms. | the blue wavelengths within the visible light spectrum are intrinisically antimicrobial and can photodynamically inactivate the cells of a wide spectrum of bacteria (gram positive and negative) and fungi. furthermore, blue light is equally effective against both drug-sensitive and -resistant members of target species and is less detrimental to mammalian cells than is uv radiation. blue light is currently used for treating acnes vulgaris and helicobacter pylori infections; the utility for deconta ... | 2016 | 27129967 |
| new β-lactamase inhibitors in the clinic. | given the serious medical burden of β-lactamases, many approaches are being used identify candidate agents for β-lactamase inhibition. here, we review two β-lactam-β-lactamase inhibitor (bl-bli) combinations, ceftolozane-tazobactam and ceftazidime-avibactam that recently entered the clinic. in addition, we focus on bl-bli combinations in preclinical development that have demonstrated activity in clinical isolates via susceptibility testing and/or in in vivo models of infection. we highlight only ... | 2016 | 27208767 |
| taxonomer: an interactive metagenomics analysis portal for universal pathogen detection and host mrna expression profiling. | high-throughput sequencing enables unbiased profiling of microbial communities, universal pathogen detection, and host response to infectious diseases. however, computation times and algorithmic inaccuracies have hindered adoption. | 2016 | 27224977 |
| ispd produces cdp-ribitol used by fktn and fkrp to transfer ribitol phosphate onto α-dystroglycan. | mutations in genes required for the glycosylation of α-dystroglycan lead to muscle and brain diseases known as dystroglycanopathies. however, the precise structure and biogenesis of the assembled glycan are not completely understood. here we report that three enzymes mutated in dystroglycanopathies can collaborate to attach ribitol phosphate onto α-dystroglycan. specifically, we demonstrate that isoprenoid synthase domain-containing protein (ispd) synthesizes cdp-ribitol, present in muscle, and ... | 2016 | 27194101 |
| the role of toll-like receptor 4 in infectious and noninfectious inflammation. | toll-like receptor 4 (tlr4) belongs to the family of pattern recognition receptors (prrs). they are highly conserved receptors that recognize conserved pathogen-associated molecular patterns (pamps), thus representing the first line of defense against infections. tlr4 has been long recognized as the sensing receptor for gram-negative lipopolysaccharide (lps). in addition, it also binds endogenous molecules produced as a result of tissue injury. hence, tlr4 represents a key receptor on which both ... | 2016 | 27293318 |
| elizabethkingia anophelis bacteremia is associated with clinically significant infections and high mortality. | unlike elizabethkingia meningoseptica, the clinical importance of e. anophelis is poorly understood. we determined the clinical and molecular epidemiology of bacteremia caused by elizabethkingia-like species from five regional hospitals in hong kong. among 45 episodes of elizabethkingia-like bacteremia, 21 were caused by elizabethkingia, including 17 e. anophelis, three e. meningoseptica and one e. miricola; while 24 were caused by other diverse genera/species, as determined by 16s rrna gene seq ... | 2016 | 27185741 |
| a gene of the β3-glycosyltransferase family encodes n-acetylglucosaminyltransferase ii function in trypanosoma brucei. | the bloodstream form of the human pathogen trypanosoma brucei expresses oligomannose, paucimannose, and complex n-linked glycans, including some exceptionally large poly-n-acetyllactosamine-containing structures. despite the presence of complex n-glycans in this organism, no homologues of the canonical n-acetylglucosaminyltransferase i or ii genes can be found in the t. brucei genome. these genes encode the activities that initiate the elaboration of the manα1-3 and manα1-6 arms, respectively, o ... | 2016 | 27189951 |
| production of functionally active and immunogenic non-glycosylated protective antigen from bacillus anthracis in nicotiana benthamiana by co-expression with peptide-n-glycosidase f (pngase f) of flavobacterium meningosepticum. | bacillus anthracis has long been considered a potential biological warfare agent, and therefore, there is a need for a safe, low-cost and highly efficient anthrax vaccine with demonstrated long-term stability for mass vaccination in case of an emergency. many efforts have been made towards developing an anthrax vaccine based on recombinant protective antigen (rpa) of b. anthracis, a key component of the anthrax toxin, produced using different expression systems. plants represent a promising reco ... | 2016 | 27101370 |
| polymicrobial infection of the cornea due to contact lens wear. | a 38-year-old male presented with pain and redness in his left eye. he had a history of wearing contact lenses. his ophthalmic examination revealed a large corneal ulcer with surrounding infiltrate. cultures were isolated from the contact lenses, lens solutions, storage cases, and conjunctivae of both eyes and also corneal scrapings of the left eye. fortified vancomycin and amikacin drops were started hourly. culture results of conjunctivae of each eye and left cornea were positive for pseudomon ... | 2016 | 27800266 |
| association between elevated alanine aminotransferase and urosepsis in children with acute pyelonephritis. | the aim of this study is to investigate the association between elevated alanine aminotransferase (alt) and urosepsis in children with acute pyelonephritis (apn). | 2016 | 27066449 |
| mosquito c-type lectins maintain gut microbiome homeostasis. | the long-term evolutionary interaction between the host immune system and symbiotic bacteria determines their cooperative rather than antagonistic relationship. it is known that commensal bacteria have evolved a number of mechanisms to manipulate the mammalian host immune system and maintain homeostasis. however, the strategies employed by the microbiome to overcome host immune responses in invertebrates still remain to be understood. here, we report that the gut microbiome in mosquitoes utilize ... | 2016 | 27170846 |
| draft genome sequences of strains representing each of the elizabethkingia genomospecies previously determined by dna-dna hybridization. | draft genome sequences of elizabethkingia meningoseptica and representatives of each of its four historically described genomospecies were sequenced here. preliminary analysis suggests that elizabethkingia miricola belongs to genomospecies 2, and both elizabethkingia anophelis and elizabethkingia endophytica are most similar to genomospecies 1. | 2016 | 26966213 |
| native conformation and canonical disulfide bond formation are interlinked properties of hiv-1 env glycoproteins. | we investigated whether there is any association between a native-like conformation and the presence of only the canonical (i.e., native) disulfide bonds in the gp120 subunits of a soluble recombinant human immunodeficiency virus type 1 (hiv-1) envelope (env) glycoprotein. we used a mass spectrometry (ms)-based method to map the disulfide bonds present in nonnative uncleaved gp140 proteins and native-like sosip.664 trimers based on the bg505 env gene. our results show that uncleaved gp140 protei ... | 2016 | 26719247 |
| biochemical characterization of cps-1, a subclass b3 metallo-β-lactamase from a chryseobacterium piscium soil isolate. | cps-1 is a subclass b3 metallo-β-lactamase from a chryseobacterium piscium isolate collected from soil, showing 68% amino acid identity to the gob-1 enzyme. cps-1 was overproduced in escherichia coli rosetta (de3), purified by chromatography, and biochemically characterized. this enzyme exhibits a broad-spectrum substrate profile, including penicillins, cephalosporins, and carbapenems, which overall resembles those of l1, gob-1, and acquired subclass b3 enzymes aim-1 and smb-1. | 2016 | 26666948 |
| evaluation of the filmarray blood culture identification panel: results of a multicenter controlled trial. | sepsis is a major cause of morbidity, mortality, and increased medical expense. rapid diagnosis improves outcomes and reduces costs. the filmarray blood culture identification panel (biofire diagnostics llc, salt lake city, ut), a highly multiplexed pcr assay, can identify 24 etiologic agents of sepsis (8 gram-positive, 11 gram-negative, and 5 yeast species) and three antimicrobial resistance genes (meca, vana/b, and blakpc) from positive blood culture bottles. it provides results in about 1 h w ... | 2016 | 26739158 |
| comparative study of plasma endotoxin with procalcitonin levels in diagnosis of bacteremia in intensive care unit patients. | both procalcitonin (pct) and plasma endotoxin levels cannot be solely used for a definite diagnosis of bacteremia or sepsis, and there has been few study comparing the values of the two biomarkers for the diagnosis of bacteremia. the aim of this study was to identify bacteria causing bacteremia and evaluate the role of the two biomarkers in the diagnosis of bacteremia in intensive care unit (icu). | 2016 | 26879015 |
| functional and structural analysis of a β-glucosidase involved in β-1,2-glucan metabolism in listeria innocua. | despite the presence of β-1,2-glucan in nature, few β-1,2-glucan degrading enzymes have been reported to date. recently, the lin1839 protein from listeria innocua was identified as a 1,2-β-oligoglucan phosphorylase. since the adjacent lin1840 gene in the gene cluster encodes a putative glycoside hydrolase family 3 β-glucosidase, we hypothesized that lin1840 is also involved in β-1,2-glucan dissimilation. here we report the functional and structural analysis of lin1840. a recombinant lin1840 prot ... | 2016 | 26886583 |
| pathogens in ornamental waters: a pilot study. | in parks, ornamental waters of easy access and populated with animals are quite attractive to children and yet might hide threats to human health. the present work focuses on the microbiota of the ornamental waters of a lisboa park, characterized during 2015. the results show a dynamic microbiota integrating human pathogens such as klebsiella pneumoniae, aeromonas spp. and enterobacter spp., and also antibiotic resistant bacteria. k. pneumoniae and aeromonas spp. were present as planktonic and b ... | 2016 | 26891309 |
| n-glycosylation improves the pepsin resistance of histidine acid phosphatase phytases by enhancing their stability at acidic phs and reducing pepsin's accessibility to its cleavage sites. | n-glycosylation can modulate enzyme structure and function. in this study, we identified two pepsin-resistant histidine acid phosphatase (hap) phytases from yersinia kristensenii (ykappa) and yersinia rohdei (yrappa), each having an n-glycosylation motif, and one pepsin-sensitive hap phytase from yersinia enterocolitica (yeappa) that lacked an n-glycosylation site. site-directed mutagenesis was employed to construct mutants by altering the n-glycosylation status of each enzyme, and the mutant an ... | 2016 | 26637601 |
| expression, purification and biological characterization of the extracellular domain of cd40 from pichia pastoris. | cd40, also called bp50, is a novel member of the tnf receptor superfamily. based on its important role in multiple physiological and pathological processes, the cd40 signaling pathway has become a vital target for treating transplantation, autoimmune diseases and cancers. this study generated a protein fragment that disrupts this signaling pathway. | 2016 | 26809818 |
| high-quality genome sequence and description of chryseobacterium senegalense sp. nov. | strain ff12(t) was isolated from the mouth of a west african lungfish (protopterus annectens) in senegal. maldi-tof-ms did not provide any identification. this strain exhibited a 97.97% 16s rrna sequence identity with kaistella flava. using a polyphasic study including phenotypic and genomic analyses, strain ff12(t) is gram-negative, aero-anaerobic, oxidase-positive, non-motile, non-spore-forming, and exhibited a genome of 4,397,629 bp with a g+c content of 35.1% that coded 4,001 protein-coding ... | 2016 | 26933502 |
| a novel mva-mediated pathway for isoprene production in engineered e. coli. | to deal with the increasingly severe energy crisis and environmental consequences, biofuels and biochemicals generated from renewable resources could serve as a promising alternative for replacing petroleum as a source of fuel and chemicals, among which isoprene (2-methyl-1,3-butadiene) in particular is of great significance in that it is an important platform chemical, which has been used in industrial production of synthetic rubber for tires and coatings or aviation fuel. | 2016 | 26786050 |
| glycomic characterization of induced pluripotent stem cells derived from a patient suffering from phosphomannomutase 2 congenital disorder of glycosylation (pmm2-cdg). | pmm2-cdg, formerly known as congenital disorder of glycosylation-ia (cdg-ia), is caused by mutations in the gene encoding phosphomannomutase 2 (pmm2). this disease is the most frequent form of inherited cdg-diseases affecting protein n-glycosylation in human. pmm2-cdg is a multisystemic disease with severe psychomotor and mental retardation. in order to study the pathophysiology of pmm2-cdg in a human cell culture model, we generated induced pluripotent stem cells (ipscs) from fibroblasts of a p ... | 2016 | 26785728 |
| a yeast strain associated to anopheles mosquitoes produces a toxin able to kill malaria parasites. | malaria control strategies are focusing on new approaches, such as the symbiotic control, which consists in the use of microbial symbionts to prevent parasite development in the mosquito gut and to block the transmission of the infection to humans. several microbes, bacteria and fungi, have been proposed for malaria or other mosquito-borne diseases control strategies. among these, the yeast wickerhamomyces anomalus has been recently isolated from the gut of anopheles mosquitoes, where it release ... | 2016 | 26754943 |
| probing substrate binding to the metal binding sites in metallo-β-lactamase l1 during catalysis. | metal ions in metallo-β-lactamases (mbls) play a major role in catalysis. in this study we investigated the role of the metal ions in the zn1 and zn2 sites of mbl l1 during catalysis. a znco (with zn(ii) in the invariant zn1 site and co(ii) in the zn2 site) analog of mbl l1 was prepared by using a biological incorporation method. extended x-ray absorption fine structure (exafs) spectroscopic studies were used to confirm that the znco analog was prepared. to study the roles of the zn(ii) and co(i ... | 2016 | 27087914 |
| b1-metallo-β-lactamases: where do we stand? | metallo-β-lactamases (mbls) are class bβ-lactamases that hydrolyze almost all clinically-availableβ-lactam antibiotics. mbls feature the distinctive αβ/βα sandwich fold of the metallo-hydrolase/oxidoreductase superfamily and possess a shallow active-site groove containing one or two divalent zinc ions, flanked by flexible loops. according to sequence identity and zinc ion dependence, mbls are classified into three subclasses (b1, b2 and b3), of which the b1 subclass enzymes have emerged as the m ... | 2016 | 26424398 |
| n-glycosylation profiling of colorectal cancer cell lines reveals association of fucosylation with differentiation and caudal type homebox 1 (cdx1)/villin mrna expression. | various cancers such as colorectal cancer (crc) are associated with alterations in protein glycosylation. crc cell lines are frequently used to study these (glyco)biological changes and their mechanisms. however, differences between crc cell lines with regard to their glycosylation have hitherto been largely neglected. here, we comprehensively characterized the n-glycan profiles of 25 different crc cell lines, derived from primary tumors and metastatic sites, in order to investigate their potent ... | 2016 | 26537799 |
| etymologia: elizabethkingia. | | 2016 | 27057563 |
| waterborne elizabethkingia meningoseptica in adult critical care. | elizabethkingia meningoseptica is an infrequent colonizer of the respiratory tract; its pathogenicity is uncertain. in the context of a 22-month outbreak of e. meningoseptica acquisition affecting 30 patients in a london, uk, critical care unit (3% attack rate) we derived a measure of attributable morbidity and determined whether e. meningoseptica is an emerging nosocomial pathogen. we found monomicrobial e. meningoseptica acquisition (n = 13) to have an attributable morbidity rate of 54% (syste ... | 2016 | 26690562 |
| melanotic pathology and vertical transmission of the gut commensal elizabethkingia meningoseptica in the major malaria vector anopheles gambiae. | the resident gut flora is known to have significant impacts on the life history of the host organism. endosymbiotic bacterial species in the anopheles mosquito gut are potent modulators of sexual development of the malaria parasite, plasmodium, and thus proposed as potential control agents of malaria transmission. | 2013 | 24098592 |
| emergence of carbapenem resistant non-fermenting gram-negative bacilli isolated in an icu of a tertiary care hospital. | the emergence and spread of multi-drug resistant (mdr) non-fermenting gram-negative bacilli (nfgnb) in intensive care units (icu) and their genetic potential to transmit diverse antibiotic resistance regardless of their ability to ferment glucose poses a major threat in hospitals. the complex interplay of clonal spread, persistence, transmission of resistance elements and cell-cell interaction leads to the difficulty in controlling infections caused by these multi drug-resistant strains. among n ... | 2017 | 28273965 |
| aerobic cloacal and pharyngeal bacterial flora in six species of free-living birds. | the purpose of this study was to investigate the culturable aerobic pharyngeal and cloacal bacterial flora of free-living birds, to determine the physiological bacterial microbiota, to identify possible interactions between feeding behaviour and the composition of the pharyngeal and cloacal microflora and to investigate the occurrence of pathogenic bacteria. | 2014 | 25176477 |
| development of a multiplex polymerase chain reaction to detect five common gram-negative bacteria of aquatic animals. | a multiplex polymerase chain reaction (m-pcr) technique was developed as a rapid and accurate diagnostic tool for identifying five major gram-negative bacilli -vibrio vulnificus, v. parahaemolyticus, aeromonas hydrophila, chryseobacterium meningosepticum and edwardsiella tarda- that cause major diseases in cultured aquatic animals in taiwan. the expected amplicons for v. vulnificus, v. parahaemolyticus, a. hydrophila, c. meningosepticum and e. tarda were 410, 368, 685, 180 and 230bp, respectivel ... | 2012 | 22571515 |