| comparison of inhibitory effects of prolinal-containing peptide derivatives on prolyl endopeptidases from bovine brain and flavobacterium. | the inhibitory effects of proline-containing peptides and their derivatives on prolyl endopeptidases from flavobacterium meningosepticum and bovine brain were compared. replacement of the carboxyl terminal proline in n-blocked peptides with prolinal resulted in remarkable decreases in ki values for both prolyl endopeptidases. further reduction of the prolinal to prolinol led to a decrease in their inhibitory effects. z-pro-, z-val-, and suc-pro-prolinals were similarly inhibitory for both the en ... | 1985 | 3908451 |
| deglycosylation of alpha 1-proteinase inhibitor by endo-beta-n-acetylglucosaminidase f. | the glycosidase endo-beta-n-acetylglucosaminidase f (endo f) from flavobacterium meningosepticum was used for the deglycosylation of rat alpha 1-proteinase inhibitor (alpha 1 pi). alpha 1 pi containing three oligosaccharide side chains of the complex type was isolated from rat serum or from the medium of rat hepatocyte primary cultures. high-mannose-type alpha 1 pi or hybrid-type alpha 1 pi was isolated from the media of hepatocytes treated with 1-deoxymannojirimycin or swainsonine, respectively ... | 1985 | 3935164 |
| flavobacterium meningosepticum ventriculitis: in vivo and in vitro results with the combinations rifampicin-erythromycin and mezlocillin-cefoxitin. | a case of flavobacterium meningosepticum ventriculitis is described in which the failure of therapy with a combination of rifampicin and erythromycin is attributed to inadequate antibiotic levels in cerebrospinal fluid. the successful eradication of the organism was achieved with the use of mezlocillin and cefoxitin given by the intravenous and intraventricular route. in vitro sensitivity tests of recently isolated strains of flavobacterium meningosepticum suggested that the combination mezlocil ... | 1982 | 6217065 |
| in vitro antimicrobial activity of ceftizoxime against glucose-nonfermentative gram-negative rods. | ceftizoxime, a new cephalosporin, was active against pseudomonas cepacia, flavobacterium meningosepticum, alcaligenes faecalis, and acinetobacter calcoaceticus and was more potent against pseudomonas aeruginosa and pseudomonas putida than was carbenicillin. | 1981 | 6269480 |
| biological activities of partially purified elastase produced by flavobacterium meningosepticum. | injection of viable cells of flavobacterium meningosepticum at doses of 10(6) colony-forming units/eye caused ophthalmia in rabbits' corneas. elastase, free of protease activity, was partially purified from broth cultures of f. meningosepticum atcc12535 and tms516. the optimum ph of the elastase activity was about 8.0. the activity was suppressed by ni, zn, co, edta, and o-phenanthroline, and accelerated by cu and fe. the minimum dose of the elastase causing ophthalmia in the eyes of rabbits was ... | 1984 | 6335217 |
| requirements of cleavage of high mannose oligosaccharides in glycoproteins by peptide n-glycosidase f. | peptide n-glycosidase from flavobacterium meningosepticum cleaves complex as well as neutral glycoproteins (plummer, t.h., jr., elder, j.h., alexander, s., phelan, a.w., and tarentino, a.l. (1984) j. biol. chem. 259, 10700-10704). examples of neutral glycoprotein substrates include ribonuclease b (one high mannose oligosaccharide chain) and yeast external invertase (nine chains/invertase subunit). the rate of deglycosylation by the glycosidase was greatly enhanced if the glycoprotein substrate w ... | 1986 | 3941069 |
| successful treatment of prosthetic valve endocarditis due to flavobacterium meningosepticum. | | 1985 | 4049054 |
| collaborative evaluation of the abbott avantage system for identification of frequently isolated nonfermentative or oxidase-positive gram-negative bacilli. | the capability of the abbott avantage system to identify 10 species of commonly isolated glucose nonfermentative or oxidase-positive gram-negative bacilli in a 5-h test period was evaluated in a collaborative study. the avantage nonenteric data base uses 20 biochemical test reactions performed in an expanded abbott bacterial identification cartridge plus the results of a manual oxidase test. the species included in the avantage data base are acinetobacter anitratus, acinetobacter iwoffi, aeromon ... | 1984 | 6392323 |
| [meningitis caused by flavobacterium meningosepticum in premature and newborn infants]. | | 1984 | 6398420 |
| the mechanism of experimental adipocere formation: hydration and dehydrogenation in microbial synthesis of hydroxy and oxo fatty acids. | the enzyme preparations from flavobacterium meningosepticum solubilized by sonication catalyzed not only hydration of oleic acid to produce 10-hydroxystearic acid but dehydrogenation of this product. the mechanism of the hydration and dehydrogenation was proved by gas chromatography-mass spectrometry of 10-hydroxy and 10-oxostearic acids produced in the presence of d2o or h2(18)o. the activity of these enzymes was increased by preincubating flavobacterium meningosepticum with oleic acid. | 1988 | 3410394 |
| in vitro activity of cefoperazone against nonfermenters and aeromonas hydrophila. | the in vitro activity of cefoperazone against 380 strains (33 species and unnamed groups) of nonferenters and 20 strains of aeromonas hydrophila was studied by a microdilution method of determining minimal inhibitory concentrations. for comparison, the activities of ampicillin, ticarcillin, and cefamandole were simultaneously studied. cefoperazone was the most active drug against pseudomonas sp., achromobacter xylosoxidans, flavobacterium meningosepticum. flavobacterium sp. (group iib), group iv ... | 1980 | 6448581 |
| [an in vitro study of susceptibility of flavobacterium meningosepticum to antibiotics]. | the present study concern in vitro observation on the susceptibility to 20 antibacterial agents of the strains of flavobacterium meningosepticum from clinical specimens at juntendo university hospital during the 1 year period of 1981. the tests for susceptibility of the 144 strains to the drugs were all performed by the serial 2-fold agar plate dilution method on mueller hinton agar (difco), standardized by the japan society of chemotherapy using the microplanter apparatus with one loop of appro ... | 1983 | 6551461 |
| antibiotic resistance pattern of flavobacterium meningosepticum. | | 1986 | 3489618 |
| direct dilution sampling, quantitation, and microbial assessment of open-system ventilation circuits in intensive care units. | in a systematic approach, 37 duplicate samples of open system circuits (bennett ma-1 ventilators) of patients in medical and surgical intensive care units were processed by direct and serial (apha guidelines) dilutions. the paired difference test on 15 of the in-use circuitry solution samples indicated no difference between the direct and serial dilution methods (p less than 0.001). seventy-seven additional respiratory therapy circuitry samples from similar intensive care patients were analyzed ... | 1983 | 6575015 |
| trimethoprim sulphamethoxazole in neonatal flavobacterium meningosepticum infection. | during an outbreak of flavobacterium meningosepticum septicaemia in a neonatal intensive care unit 9 infants were treated with intravenous trimethoprim sulphamethoxazole. bacteriological cure was achieved in 8 patients; one infant died of massive intraventricular haemorrhage on the first day of treatment. apart from prolonged persistence of pre-existing thrombocytopenia there was no evidence of side effects. trimethoprim sulphamethoxazole should be considered in the treatment of neonatal f menin ... | 1984 | 6611137 |
| identification and distribution of flavobacterium meningosepticum in clinical material. | during the 19 year period ending december 1984, 82 (1.7%) of 4840 strains of gram-negative non-fermentative bacteria submitted to the national collection of type cultures (nctc) for computer-assisted identification were flavobacterium meningosepticum. these figures suggest either that f. meningosepticum occurs only rarely in clinical material in the uk, or that when it does occur it is not always recognized. the sources from which the nctc strains were isolated are reported and also the characte ... | 1987 | 3571032 |
| comparative in vitro antibacterial activities of two new oral cephalosporins, ceftetrame (ro 19-5247) and cefetamet (ro 15-8074). | the in vitro activities of two new oral cephalosporins, ceftetrame (ro 19-5247) and cefetamet (ro 15-8074), were tested against 990 clinical bacterial isolates in comparison with that of cephalexin. both compounds were more active than cephalexin against gram-negative bacteria, inhibiting most isolates of the family enterobacteriaceae at concentrations of less than or equal to 4 micrograms/ml, but were not active against acinetobacter species, most pseudomonas species, campylobacter jejuni, and ... | 1987 | 3579264 |
| antimicrobial susceptibility of flavobacterium meningosepticum strains identified by dna-dna hybridization. | antimicrobial susceptibilities of 52 strains of flavobacterium meningosepticum were determined by an agar plate dilution technique and by a tablet diffusion method. no useful susceptibility differences between the two main dna relatedness groups within f. meningosepticum were found. all strains produced beta-lactamase and were resistant to most beta-lactam antibiotics. ciprofloxacin, rifampicin and clindamycin appear to be the most promising for treating infections caused by f. meningosepticum. | 1987 | 3591313 |
| crossed immunoelectrophoretic analysis of flavobacterium meningosepticum and allied flavobacterium taxa. | a total of 55 different antigens were demonstrated in pooled lysate of six flavobacterium meningosepticum strains by crossed immunoelectrophoresis against homologous rabbit antibody. the six strains used represented the two dna relatedness groups within this species. all 52 strains of f. meningosepticum investigated were found to cross-react with 53 to 55 of these f. meningosepticum reference antigens. using this f. meningosepticum reference system, cross-reactions between the f. meningosepticum ... | 1987 | 3673581 |
| [bacteremia due to flavobacterium meningosepticum. clinical and bacteriological aspects of patients (author's transl)]. | | 1980 | 6782178 |
| isolation of a pseudomonas stutzeri strain that degrades o-xylene. | a pseudomonas stutzeri strain capable of growing on o-xylene was isolated from enrichment cultures. the organism grew on 2,3- and 3,4-dimethylphenol but not on 2-methylbenzyl alcohol, o-tolualdehyde, or o-toluate. p. stutzeri was not able to utilize m-xylene, p-xylene, or 1,2,4-trimethylbenzene, but growth was observed in the presence of the corresponding alcohols and acids. from the pseudomonas cultures supplied with o-xylene, 2,3-dimethylphenol was isolated and identified. when resting p. stut ... | 1987 | 3674872 |
| neonatal meningitis by flavobacterium meningosepticum. | | 1987 | 3689534 |
| the importance of temperature for the growth of flavobacterium meningosepticum. | | 1966 | 6006195 |
| a clinical analysis of 10 cases of postoperative infection with flavobacterium meningosepticum. | | 1967 | 6016783 |
| the effects of temperature and ph on the growth of eight enteric and nine glucose non-fermenting species of gram-negative rods. | we studied the heat resistance and the range of growth temperature o gram-negative rods to find one of the bacterial factors governing their infectivity in exogenous and endogenous infections in predisposed patients. escherichia coli, klebsiella pneumoniae, serratia marcescens, pseudomonas aeruginosa, and acinetobacter calcoaceticus grew equally well at 25, 30, 37, and 42 c. among other sugar non-fermenting gram-negative rods, six species showed suppressed growth at either or both ends of the in ... | 1982 | 7087800 |
| an epidemiological study of hospital infection with flavobacterium meningosepticum. | | 1967 | 6016789 |
| antimicrobial treatment of flavobacterium meningosepticum infection. | a six-month-old boy in hospital developed septicaemia and meningitis due to flavobacterium meningosepticum type a. although the organism was susceptible to amikacin, chloramphenicol and sulphonamides when tested by the disc diffusion method, therapy with these drugs was ineffective. intravenous therapy with cotrimoxazole gave complete recovery. various strains of flavobacteria including f. meningosepticum type b were isolated from the environment. antimicrobial susceptibilities of patients and e ... | 1983 | 6197021 |
| demonstration of peptide:n-glycosidase f activity in endo-beta-n-acetylglucosaminidase f preparations. | endo-beta-n-acetylglucosaminidase f preparations from flavobacterium meningosepticum have been found to contain peptide:n-glycosidase activity. only the second activity, designated as peptide:n-glycosidase f, readily cleaves the beta-aspartylglycosylamine linkage of a fetuin triantennary complex glycopeptide, as shown by the isolation of the corresponding carbohydrate-free peptide containing aspartic acid and of an intact oligosaccharide with a di-n-acetylchitobiosyl moiety at the reducing end. ... | 1984 | 6206060 |
| [isolation, identification and clinical significance of species of the flavobacterium genus]. | strictly aerobic gram negative bacteria are found more and more often in human pathological specimens and in the environment. amongst these bacteria, flavobacterium poses problems of bacteriological diagnosis as their precise taxonomy is still recent. in this study, conducted on 321 strains of flavobacterium from various sources, but essentially from patients in the intensive care unit, the authors define the methods and features of identification and the clinical significance of the species of ... | 1983 | 6354010 |
| evidence showing that a proline-specific endopeptidase has an absolute requirement for a trans peptide bond immediately preceding the active bond. | the proline-specific endopeptidase (ec 3.4.21.26) from flavobacterium meningosepticum is specific for the cleavage of peptide bonds on the c-terminal side of prolyl residues. such bonds will normally exist in the all-trans configuration. however, the preceding peptide bond in the sequence (i.e., on the n-terminal side of the prolyl residue) will exist as a mixture of cis and trans forms in solution. in this study, the activity of the proline-specific endopeptidase toward the substrates n-cbz-gly ... | 1983 | 6354257 |
| meningitis in children of riyadh. | one hundred and forty cases of meningitis admitted to the children's hospital in riyadh from june 1980 to the end of may 1982 were included in a prospective study. sixty-seven percent were under 1 year of age and 90% were under 5 years old. one hundred and twenty-four (88.6%) had purulent csf, 12 (8.6%) had viral and four (2.9%) had tuberculous meningitis. e. coli was the commonest causative organism in the neonates. after that age s. pneumoniae was the predominant isolate. the implication of in ... | 1984 | 6398834 |
| icu-acquired pneumonia due to flavobacterium meningosepticum. | | 1974 | 4406261 |
| a comparison of strains of king's group iib of flavobacterium with flavobacterium meningosepticum. | | 1974 | 4599090 |
| an endo-acting proline-specific oligopeptidase from treponema denticola atcc 35405: evidence of hydrolysis of human bioactive peptides. | an endo-acting proline-specific oligopeptidase (prolyl oligopeptidase [popase], ec 3.4.21.26) was purified to homogeneity from the triton x-100 extracts of cells of treponema denticola atcc 35405 (a human oral spirochete) by a procedure that comprised five successive fast protein liquid chromatography steps. the popase is a cell-associated 75- to 77-kda protein with an isoelectric point of ca. 6.5. the enzyme hydrolyzed (optimum ph 6.5) the pro-pna bond in carbobenzoxy-gly-pro-p-nitroanilide (z- ... | 1994 | 7523301 |
| bacterial contamination of aerosol solutions containing antibiotics. | in an investigation of microbial contamination of aerosol solutions containing antibiotics (hospital pharmaceutical preparations, no preservatives added), five of six residual solutions after multiple use for 7 days were contaminated at a concentration of 10(6) viable counts/ml. contaminants were glucose-nonfermenting gram-negative bacilli such as pseudomonas cepacia and flavobacterium meningosepticum. the major contaminant, p. cepacia, multiplied rapidly in the aerosol solution under simulated ... | 1995 | 7541888 |
| self-induced bacteremia. case report. | we describe a patient with perforated appendicitis who postoperatively suffered repeated episodes of shaking chills and temperature spikes. initial blood cultures yielded growth of flavobacterium meningosepticum, pseudomonas putida and pseudomonas paucimobilis, and succeeding blood cultures growth of pseudomonas acidoverans. these bacteria in combination led to a suspicion of self-inoculation of contaminated water through an intravenous catheter. antibiotic treatment had no effect on the symptom ... | 1995 | 7546651 |
| [cerebrospinal meningitis due to flavobacterium meningosepticum in an infant]. | | 1973 | 4731040 |
| flavobacterium meningosepticum. | lavobacterium meningosepticum is an opportunistic pathogen of low virulence found in the hospital environment in water-containing equipment. of primary importance is its role in outbreaks of neonatal meningitis which tends to be severe with a high mortality rate and serious sequelae. changing all equipment concerned with humidifying or administering gases every 24 hours can help prevent these outbreaks in neonatal nurseries. treatment is difficult because of the resistance of f. meningosepticum ... | 1984 | 6563023 |
| preparation of deglycosylated egg white avidin. | a simple procedure for the preparation of deglycosylated avidin is described. commercially obtained avidin was treated with a mixed microbial culture. the cells were capable of growing on the oligosaccharide residues, but generally ignored the polypeptide portion of the egg white glycoprotein. the resultant deglycosylated avidin retained its biotin-binding characteristics. the major bacterial strain (strain bech080), responsible for the deglycosylation, was isolated. on the basis of elementary b ... | 1995 | 7625822 |
| crystal structure of endo-beta-n-acetylglucosaminidase h at 1.9 a resolution: active-site geometry and substrate recognition. | endo-beta-n-acetylglucosaminidase h (endo h), an endoglycosidase secreted by streptomyces plicatus, hydrolyzes the glycosidic bond between the core n-acetyglucosamine residues of asparagine-linked high-mannose oligosaccharides. endo h is a commonly used reagent in glycobiology research, including the characterization of oligosaccharides in glycoproteins. on-going crystallographic studies of endo h and related endoglycosidases are aimed at identifying the molecular features that determine the dif ... | 1995 | 7663942 |
| neonatal meningitis caused by flavobacterium meningosepticum. | | 1973 | 4773223 |
| substrate specificity of flavobacterium meningosepticum endo f2 and endo f3: purity is the name of the game. | | 1994 | 7734840 |
| meningitis caused by flavobacterium meningosepticum after transsphenoidal hypophysectomy with recovery. | adult meningitis caused by flavobacterium meningosepticum is rare. five cases have been reported in the literature. the case reported herein developed in a woman after a transsphenoidal hypophysectomy. the isolate was resistant to erythromycin. treatment with oral rifampicin combined with intravenous chloramphenicol and cefoperazone resulted in complete recovery. | 1983 | 6623338 |
| flavobacterium meningosepticum from neonatal meningitis. | | 1970 | 5311075 |
| successful treatment of neonatal flavobacterium meningosepticum infection. | an 8-day-old, 2.48-kg, 35-week gestation infant developed neonatal sepsis and meningitis due to flavobacterium meningosepticum serotype f. treatment with a new antibiotic, azlocillin, in combination with chloramphenicol, led to complete recovery. | 1983 | 6628455 |
| rifampin therapy for brucellosis, flavobacterium meningitis, and cutaneous leishmaniasis. | rifampin has a broad antibacterial spectrum. at high concentrations it also is active in vitro against protozoa, i.e., different species of leishmania. rifampin has been used against bacterial and occasionally protozoal infections. addition of rifampin to a tetracycline regimen was found to reduce the number of relapses in patients with acute and chronic brucellosis. in a few cases of meningitis due to flavobacterium meningosepticum, which failed to respond to other drugs, patients were treated ... | 1983 | 6635449 |
| proline-specific dipeptidyl aminopeptidase from flavobacterium meningosepticum. | a proline-specific dipeptidyl aminopeptidase was highly purified from cell-free extract of flavobacterium meningosepticum by a series of column chromatographies on deae-sephadex a-50, sephadex g-150, hydroxyapatite, and a second gel filtration on sephadex g-150. the enzyme was most active at ph 7.4-7.8 for both gly-pro-beta-naphthylamide (gly-pro-2-nnap) and gly-pro-p-nitroanilide (gly-pro-pna) and was stable between ph 7 and 9.5. the enzyme was markedly inhibited by diisopropylphosphofluoridate ... | 1982 | 6749829 |
| endo-beta-n-acetylglucosaminidase f: endoglycosidase from flavobacterium meningosepticum that cleaves both high-mannose and complex glycoproteins. | we have detected an endoglycosidase activity produced by flavobacterium meningosepticum. this enzyme, named endo f, cleaves glycans of both the high-mannose and the complex type linked through asparagine to the protein backbone. the data indicate that cleavage occurs via hydrolysis of the glycosidic bond of the n,n'-diacetylchitobiose core structure adjacent to asparagine, similar to that due to endo h and endo d. extreme variability was noted in the availability of this cleavage site among n-li ... | 1982 | 6812050 |
| flavobacterium meningosepticum. a bacteriological, epidemiological and clinical study. | | 1970 | 5454400 |
| the sensitivity of flavobacterium meningosepticum to antibiotics at different temperatures. an in vitro study. | | 1967 | 5584517 |
| microbial endoglycosidases for analyses of oligosaccharide chains in glycoproteins. | microbial endoglycosidases are useful for elucidating the structure and function of the oligosaccharide chains of glycoconjugates. most of the microbial endo-beta-n-acetylglucosaminidases including endo-h can preferentially act on high-mannose type chains of asparagine-linked oligosaccharides of various glycoproteins. among them, flavobacterium sp. enzyme is produced in large amounts by the inducing cultivation. using this enzyme, the role of oligosaccharide chains in various microbial glycoenzy ... | 1994 | 7822234 |
| flavobacterium meningosepticum isolated from outside hospital surroundings and during routine examination of patient specimens. | | 1969 | 5822968 |
| neonatal meningitis caused by flavobacterium meningosepticum type f. | two infants, one premature and one full-term, died from meningitis due to flav. meningosepticum type f. careful bacteriological studies are required to prevent possible confusion with other gram-negative bacilli which may cause meningitis in this age group. | 1966 | 5904987 |
| resistance to and hydrolysis of imipenem in nosocomial strains of flavobacterium meningosepticum. | | 1994 | 7889956 |
| evaluation of minocycline and cefuzonam for antimicrobial activity against clinical isolates. | the antibacterial activity of minocycline (mino) and that of cefuzonam (czon) were assessed with clinical isolates of 19 species, and compared with that of other antibiotics. mino was highly active against methicilli-sensitive staphylococcus aureus (mssa), neisseria gonorrhoeae, moraxella (branhamella) catarrhalis, haemophilus influenzae, helicobacter pylori, flavobacterium meningosepticum, acinetobacter calcoaceticus, peptostreptococcus spp. and propionibacterium acnes, but not as effective aga ... | 1994 | 7933531 |
| [hospital infection caused by flavobacterium meningosepticum]. | | 1982 | 7078943 |
| successful therapy with rifampin--flavobacterium meningosepticum meningitis developing while on erythromycin therapy. | | 1982 | 7173459 |
| a case of neonatal meningitis due to flavobacterium meningosepticum successfully treated with rifampin. | | 1982 | 7177894 |
| flavobacterium meningosepticum infection: an epidemiological study in a newborn nursery. | after an outbreak of flavobacterium meningitis in the newborn nursery of the university hospital, an investigation was carried out to determine the possible sources of this organisms in the nursery. various serotypes of the organism were recovered from a variety of sources such as wash basins, sinks, suction apparatus, and disinfectants in the nursery as well as the neighbouring wards. colonisation of the pharynx with this organism was demonstrated in several clinically healthy babies in the nur ... | 1981 | 7240432 |
| flavobacterium meningosepticum in the neonatal period. | we have described a term newborn infant who developed flavobacterium meningosepticum meningitis at 9 days of age. the case demonstrates the tenacity and changing antibiotic sensitivity of this organism. we used trimethoprim-sulfamethoxazole (bactrim) intravenously to obtain csf sterilization. on follow-up this child was neurologically intact. | 1981 | 7244761 |
| purification and crystallization of the endoglycosidase pngase f, a peptide:n-glycosidase from flavobacterium meningosepticum. | the endoglycosidase peptide: n-glycosidase, secreted by the gram-negative bacterium flavobacterium meningosepticum (pngase f), has been isolated, purified to homogeneity and crystallized from polyethylene glycol solutions using vapour diffusion and seeding techniques. the crystals are orthorhombic, space group p2(1)2(1)2, with unit cell dimensions a = 85.07 a, b = 85.14 a, c = 48.50 a, and are suitable for high resolution x-ray structure analysis. | 1994 | 8057384 |
| molecular cloning and characterization of prolyl endopeptidase from human t cells. | the prolyl endopeptidase (pep) gene of human t cells was amplified by the pcr method and cloned in escherichia coli. the complete gene consisted of 2,130 nucleotides corresponding to 710 amino acid residues with a calculated molecular mass of 80,750. the nucleotide sequence of this clone revealed that t cell pep dna is 48, 50, and 91% homologous to those of flavobacterium meningosepticum, aeromonas hydrophila, and porcine brain pep, respectively. this gene was fused to the lacz sequence from e. ... | 1994 | 8089089 |
| flavobacterium meningosepticum sepsis: disease due to bacteria with unusual antibiotic susceptibility. | the three cases we have presented implicate f meningosepticum as a significant pathogen causing disease in immunocompromised adult patients. since this organism is of low pathogenicity but may be found in the hospital environment, its identification as a pathogen raises the suspicion of a nosocomial source of infection, and a search for the source should be made. furthermore, with regard to the choice of antimicrobial therapy, it must be remembered that this organism is resistant to most antibio ... | 1981 | 7244763 |
| [perinatal infections with unusual pathogens. 3. flavobacterium meningosepticum]. | | 1981 | 7279415 |
| purification and characterization of a neutral zinc endopeptidase secreted by flavobacterium meningosepticum. | flavobacterium meningosepticum, elder strain (atcc 33958), secretes into the medium a neutral zinc endoprotease as a major component of the extracellular proteins. the enzyme was purified to homogeneity in a simple two-step procedure involving ammonium sulfate precipitation and hydrophobic interaction chromatography. the molecular weight of this metalloprotease was determined to be about 27,000 (p27) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. p27 was comparable to thermolysin ... | 1994 | 8185308 |
| [2 cases of flavobacterium meningosepticum infection in a neonatology ward]. | | 1980 | 7412550 |
| meningitis of infants caused by flavobacterium meningosepticum: report of a patient and analysis of 63 infections. | | 1980 | 7420510 |
| jtp-4819: a novel prolyl endopeptidase inhibitor with potential as a cognitive enhancer. | jtp-4819 ((s)-2-[[(s)-2-(hydroxyacetyl)-1-pyrrolidinyl]carbonyl]-n- phenylmethyl)-1-pyrrolidinecarboxamide) is a potent (ic50: 0.83 +/- 0.09 nm in rat brain supernatant; 5.43 +/- 0.81 nm in flavobacterium meningosepticum) and specific inhibitor of prolyl endopeptidase (pep). jtp-4819 (3 mg/kg p.o.) exhibited a strong and durable ex vivo inhibitory effect on pep in various regions of the rat brain. in addition, jtp-4819 inhibited the degradation of substance p, arginine-vasopressin, thyrotropin-r ... | 1995 | 7562510 |
| cellulitis associated with bacteremia due to flavobacterium meningosepticum. | | 1993 | 8286646 |
| [meningitis after myelography]. | lumbar myelography is still frequently used in cases of suspected lumbar radiculopathy. since 1984, iohexol has been the contrast medium of choice in myelography, and so far only a few cases of chemical meningitis have been reported. bacterial meningitis cannot be distinguished from chemical meningitis on the basis of clinical findings. a cerebrospinal fluid gram stain and culture are the only truly reliable tests in deciding the etiology of the meningitis. we describe two patients who developed ... | 1995 | 7570474 |
| [relationship between postmortem change and biological reaction]. | in the adipocere which is one of postmortem changes, some specific fatty acids possessing higher melting points together with soap play an important role in the formation of adipocere. these fatty acids were clarified to be mainly 10-hydroxystearic and 10-hydroxypalmitic acids. moreover, slight amounts of 10-oxostearic and 10-oxopalmitic acids, which have higher melting points than those of hydroxy fatty acids, exist in the adipocere as well. the substantial adipocere is formed and stabilized by ... | 1993 | 8309098 |
| [antibiotic treatment for pneumonia caused by flavobacterium meningosepticum]. | two cases of critical nosocomial pneumonia caused by flavobacterium meningosepticum (fm) were reported and both of them were successfully cured. there were 2 other cases of fm pneumonia reported in chinese literature previously, but none of them survived. it has been found that the treatment for fm respiratory infection was very difficult because of its resistance to majority of antibiotics, including the third generation cephalosporins. the symptoms of fm pneumonia are similar to those of other ... | 1994 | 7712928 |
| the major n-linked carbohydrate chains from human urokinase. the occurrence of 4-o-sulfated, (alpha 2-6)-sialylated or (alpha 1-3)-fucosylated n-acetylgalactosamine(beta 1-4)-n-acetylglucosamine elements. | the primary structure of the major n-linked carbohydrate chains attached to asn302 of urinary-type plasminogen activator (urokinase) have been determined. urokinase was completely deglycosylated with peptide-n4-(n-acetyl-beta-glucosaminyl)asparagine amidase f from flavobacterium meningosepticum. released oligosaccharides were separated from the remaining protein using gel-permeation chromatography on bio-gel p-100, and then on bio-gel p-6. fractionation of the oligosaccharides was achieved by a ... | 1995 | 7737145 |
| cloning of proline-specific endopeptidase gene from flavobacterium meningosepticum: expression in escherichia coli and purification of the heterologous protein. | proline-specific endopeptidase (pse) (ec 3.4.21.26) from flavobacterium meningosepticum was subjected to partial amino acid sequencing. according to the peptide sequences obtained, oligonucleotides were used to amplify a pse-specific dna fragment of 930 bp from f. meningosepticum genomic dna, employing the polymerase chain reaction technique. this fragment served as a molecular probe to isolate the respective gene. dna sequencing revealed that the pse gene consists of 2118 bp coding for a 78,634 ... | 1993 | 7764331 |
| enzymatic peptide synthesis by the recombinant proline-specific endopeptidase from flavobacterium meningosepticum and its mutationally altered cys-556 variant. | proline-specific endopeptidase (pse) (ec 3.4.21.26) was investigated for its potential as a catalyst in peptide synthesis. using an activated peptide ester or a peptide amide as the acyl component, the enzyme catalyzed kinetically controlled aminolysis and transpeptidation respectively, with various amino acid amides as acyl acceptors. to a certain extent the nucleophile preference reflected the amino acid preference in the s1'-position of the enzyme in peptide hydrolysis: the highest fractions ... | 1995 | 7766083 |
| novel, specific o-glycosylation of secreted flavobacterium meningosepticum proteins. asp-ser and asp-thr-thr consensus sites. | a new type of o-linked oligosaccharide has been discovered on several proteins secreted by the gram-negative bacterium flavobacterium meningosepticum, including endo f2 (three sites), endo f3 (one site), and a p40 protease (one site). the oligosaccharide moiety is covalently attached via a mannose residue to a serine or threonine at consensus sites corresponding to asp-ser* or asp-thr*-thr. preliminary characterization by mass spectroscopy revealed an oligosaccharide of 1244 da at each of the pr ... | 1995 | 7768916 |
| quinolone susceptibility of multiply-resistant flavobacterium meningosepticum clinical isolates in one urban hospital. | | 1995 | 8549290 |
| detailed structural analysis of a novel, specific o-linked glycan from the prokaryote flavobacterium meningosepticum. | in the preceding paper, preliminary analysis revealed a new type of o-linked oligosaccharide of 1244 da at each of two proposed glycosylation sites on several proteins secreted by the gram-negative bacterium flavobacterium meningosepticum (plummer, t. h., jr., tarentino, a. l., and hauer, c. r. (1995) j. biol. chem. 270, 13192-13196). in this report we detail the linkage, sequence, and branching of this unusual heptasaccharide by electrospray (es) ionization mass spectrometry (ms), and collision ... | 1995 | 7768917 |
| successful combination vancomycin and rifampin therapy in a newborn with community-acquired flavobacterium meningosepticum neonatal meningitis. | | 1995 | 8584328 |
| molecular cloning and sequence analysis of flavastacin: an o-glycosylated prokaryotic zinc metalloendopeptidase. | a new zinc metalloendopeptidase that cleaves peptides on the amino-terminal side of aspartic acid was isolated from the cultural filtrate of flavobacterium meningosepticum. the gene for this new enzyme was cloned into pbluescript, and the complete nucleotide sequence was determined. over 40% of the deduced amino acid sequence was verified independently by direct protein microsequencing. the most important structural features of this new enzyme include (i) the presence of an unusual o-linked olig ... | 1995 | 7771796 |
| investigations on the mechanism of adipocere formation and its relation to other biochemical reactions. | in the adipocere, which is one of the postmortem changes, some specific fatty acids possessing higher melting points, together with soap, play an important role in the formation of adipocere. these fatty acids were shown to be mainly 10-hydroxystearic and 10-hydroxypalmitic acids. moreover, slight amounts of 10-oxostearic and 10-oxopalmitic acids, which have higher melting points than those of hydroxy fatty acids, exist in the adipocere as well. the substantial adipocere is formed and stabilized ... | 1996 | 8690322 |
| cloning, sequencing, and expression of the dipeptidyl peptidase iv gene from flavobacterium meningosepticum in escherichia coli. | the dipeptidyl peptidase iv (dp iv, ec 3.4.14.5) gene from flavobacterium meningosepticum was cloned by southern and colony hybridizations using probes amplified by pcr, and expressed in escherichia coli dh1. e. coli dh1 harboring pfdp-h1, which was a subclone derived from the positive clone pfdp-1, showed 3.5-fold higher activity than f. meningosepticum. nucleotide sequencing analysis revealed an open reading frame of 2133 bp, coding for a protein of 711 amino acids with a predicted molecular w ... | 1995 | 7793970 |
| the laboratory diagnosis of opportunistic infections caused by uncommon bacteria in papua new guinea. | flavobacterium meningosepticum, chromobacterium violaceum and listeria monocytogenes are opportunistic pathogens of man and are occasional but important causes of infections in neonates, infants and adults in papua new guinea. mortality is high. flavobacterium meningosepticum should be suspected when a weak indole producing oxidase positive gram-negative bacillus producing a discrete yellow non-diffusing pigment at room temperature and showing a resistant antibiotic sensitivity pattern is isolat ... | 1981 | 6951339 |
| [meningitis caused by flavobacterium meningosepticum in an adult]. | | 1996 | 8756220 |
| flavobacterium meningosepticum, a pathogen in birds. | five bacterial isolates were recovered from various diseased birds (chickens, a pigeon, and a zebra finch) and were identified as flavobacterium meningosepticum. four of them were isolated in pure or nearly pure culture of samples from internal organs, and one strain was isolated in mixed culture of a tarsal joint fluid sample. except for the last case, there was no evidence of other disease agents. by using phenotypic, chemotaxonomic, and genomic methods, the strains were taxonomically characte ... | 1994 | 7814473 |
| molecular cloning and sequence analysis of flavobacterium meningosepticum glycosylasparaginase: a single gene encodes the alpha and beta subunits. | a full-length insert for the flavobacterium meningosepticum n4-(n-acetyl-beta-glucosaminyl)-l-asparagine amidase gene was located on a 2500-bp hindiii fragment and cloned into the plasmid vector pbluescript. dna sequencing revealed an open reading frame of 1020 nucleotides encoding a putative 45-amino-acid leader sequence and a deduced precursor polypeptide of 295 amino acids. in f. meningosepticum this precursor polypeptide undergoes proteolytic processing by an as yet unknown mechanism to gene ... | 1995 | 7840643 |
| prolyl aminopeptidase gene from flavobacterium meningosepticum: cloning, purification of the expressed enzyme, and analysis of its sequence. | in spite of the numerous studies regarding prolyl aminopeptidase, little is known about its mechanism and the significance of its similarity to a number of hydrolases of diverse specificity that belong to the alpha/beta hydrolase-fold family (pseudomonas 2-hydroxymuconic semialdehyde hydrolase, atropinesterase, and 2-hydroxy-6-oxophenylhexa-2,4-dienoic acid hydrolase; human and rat epoxide hydrolases). we report the cloning and sequencing of the novel prolyl aminopeptidase gene from flavobacteri ... | 1996 | 8951032 |
| [the bacteriostatic and bactericidal effect of amniotic fluid (author's transl)]. | the bacteriostatic and bactericidal effect of 100 samples of amniotic fluid (la) was studied against 5 bacterial species that are responsible for neonatal infections. these results show that there is a bacteriostatic activity in liquor. 52 specimens of liquor amnii (52%) were shown to be active against at least one of the 5 bacterial specimens studied. 32 samples of liquor (32%) were active against listeria monocytogenes, 21 (21%) against flavobacterium meningosepticum, 18 (18%) against escheric ... | 1981 | 7024394 |
| flavobacterium meningosepticum: an unusual pathogen. | | 1996 | 8979561 |
| dipeptidyl aminopeptidase iv from pseudomonas sp. wo24. | dipeptidyl aminopeptidase iv from pseudomonas sp. wo24 was purified as two molecular forms of 84 and 82-kda by sds-page. peptide mapping and n-terminal sequence analyses indicated that both proteins might be derived from the same protein, and that the 82-kda molecule might be a truncated form from the 84-kda molecule at least at the n-terminus. the dap iv gene of pseudomonas sp. wo24 was cloned and expressed in e. coli. the enzyme expressed in e. coli jm109 harboring a hybrid plasmid, pyo-6a, wi ... | 1996 | 8988635 |
| the three-dimensional structure of pngase f, a glycosylasparaginase from flavobacterium meningosepticum. | peptide:n-glycosidase f (pngase f) is an enzyme that catalyzes the complete removal of n-linked oligosaccharide chains from glycoproteins. often called an endoglycosidase, it is more correctly termed an amidase or glycosylasparaginase as cleavage is at the asparagine-sugar amide linkage. the enzyme is widely used in structure-function studies of glycoproteins. | 1994 | 7881905 |
| a cytosolic serine endopeptidase from trypanosoma cruzi is required for the generation of ca2+ signaling in mammalian cells. | an early event in the trypanosoma cruzi cell invasion process, the recruitment of host lysosomes, led us to investigate the involvement of signal transduction. infective trypomastigotes were found to contain a soluble ca2+-signaling activity for mammalian cells that is sensitive to protease inhibitors. inhibitor and substrate utilization profiles were used to purify a candidate peptidase for involvement in this process, from which we isolated a full-length cdna clone. the sequence revealed a nov ... | 1997 | 9024691 |
| septicemia occurring after colonoscopic polypectomy in a splenectomized patient taking corticosteroids. | although a transient bacteremia may occur in approximately 4% of patients after colonoscopic procedures, clinically significant bacteremia or endocarditis is exceedingly rare. to our knowledge, the case we describe herein is the first reported case of septicemia due to flavobacterium meningosepticum and escherichia coli after colonoscopic polypectomy. our patient was probably immunocompromised and, therefore, predisposed to bacteremia after the procedure, because she was asplenic, diabetic, and ... | 1994 | 7977253 |
| effect of small bowel transplantation, denervation and ischaemia on rat intestinal microflora. | the effects of denervation and warm ischaemia on quantitative and qualitative changes in small intestinal microflora following rat heterotopic small-bowel isotransplantation were assessed. animals with thiry-vella fistula, but without transplants, acted as controls. thirty and 40-fold increases in bacterial colony counts were seen in the isografts compared to controls at 2 and 7 days, respectively (p < 0.05). aerobic faecal organisms predominated at 2 and 7 days, but an overgrowth of flavobacter ... | 1994 | 7993569 |
| [infections caused by flavobacterium meningosepticum in patients in a neonatal intensive care unit]. | during a period of three and a half months 7 neonates from a neonatal intensive care unit became infected by f. meningosepticum, serotype b. the pathogens could be isolated from the tracheal secretions and--less frequently--from throat swabs, gastric juice and nose swabs. environmental sampling led to the isolation of f. meningosepticum from the humidification fluid of the respirator, from vaporizers as well as from the artificial ventilation tubing. f. mengingosepticum was found in the water of ... | 1994 | 8011057 |
| isolation of prolylendopeptidase-inhibiting peptides from bovine brain. | the peptides inhibiting prolylendopeptidase were screened and isolated from bovine brain by monitoring the inhibition of prolylendopeptidase produced by flavobacterium meningosepticum. the amino acid sequence of the peptide having the highest inhibitory activity was determined as met-pro-pro-pro-leu-pro-ala-arg-val-asp-phe-ser-leu-ala-gly-ala-leu-asn. this peptide showed the ic50 and ki values of 38.4 and 8.6 microm, respectively, for prolylendopeptidase isolated from bovine brain. | 1994 | 8048952 |
| enzymatic deglycosylation of asparagine-linked glycans: purification, properties, and specificity of oligosaccharide-cleaving enzymes from flavobacterium meningosepticum. | | 1994 | 8139511 |
| comparative activity of trovafloxacin, alone and in combination with other agents, against gram-negative nonfermentative rods. | in the first part of this study, agar dilution mics were used to test the activities of trovafloxacin, ciprofloxacin, ofloxacin, levofloxacin, sparfloxacin, clinafloxacin, ceftazidime, and imipenem against 458 gram-negative nonfermenters. the overall respective mics at which 50% of isolates are inhibited (mic50s) and mic90s were as follows: trovafloxacin, 1.0 and 16.0 microg/ml; ciprofloxacin, 2.0 and 16.0 microg/ml; ofloxacin, 2.0 and 32.0 microg/ml; levofloxacin, 1.0 and 16.0 microg/ml; sparfl ... | 1997 | 9210669 |
| ribotyping for differentiating flavobacterium meningosepticum isolates from clinical and environmental sources. | on the basis of dna-dna hybridization data, two main genomic relatedness groups (i and ii) have been reported for a geographically varied collection of 52 strains of flavobacterium meningosepticum. herein, we have shown that genomic group ii can be further divided into four subgroups (ii:1 to ii:4). to examine the taxonomic relevance of the ribosomal patterns of the 52 f. meningosepticum strains, the patterns were compared with existing dna-dna hybridization data with restriction enzymes psti an ... | 1994 | 8150962 |
| riemerella anatipestifer gen. nov., comb. nov., the causative agent of septicemia anserum exsudativa, and its phylogenetic affiliation within the flavobacterium-cytophaga rrna homology group. | the phylogenetic position of the causative agent of septicemia anserum exsudativa, now most often referred to as [moraxella] anatipestifer (brackets indicate a generically misnamed taxon) or "[pasteurella] anatipestifer," was established by performing rrna cistron similarity studies. [moraxella] anatipestifer belongs to rrna superfamily v, together with the genera flavobacterium, cytophaga, flexibacter, weeksella, capnocytophaga, and sphingobacterium. the detailed structure of rrna superfamily v ... | 1993 | 8240957 |