| comparison of an enzyme-linked immunospecific assay (elisa) with the cold complement fixation test for the serodiagnosis of brucella ovis infection. | an enzyme-linked immunospecific assay (elisa) for the serodiagnosis of brucella ovis infection in sheep is described and compared with the cold complement fixation (cf) test. elisa was performed in microtiter plates, using horse-radish peroxidase conjugated to anti-normal sheep serum globulins, and hydrogen peroxide plus o-phenylenediamine as substrate. a heated, cell-free b. ovis extract was used as antigen in both tests. elisa was easier to perform, distinguished better between positive and ne ... | 1984 | 16031008 |
| a comparison of three serological tests for the diagnosis of b. ovis infection in rams. | the complement fixation test (cft), the enzyme labelled immunosorbent assay (elisa) and the gel diffusion precipitin test (gd) were compared, for the diagnosis of brucella ovis infection in rams. the sensitivities of the tests in 109 rams which were shedding b. ovis in their semen were: cft 96.3%; elisa 97.2%; gd 91.7%. the specificities of the tests in 141 rams from non-infected flocks were: cfi 99.3%; elisa 98.6%; gd 100%. predictive values of the three tests were measured in 285 rams from inf ... | 1984 | 16031047 |
| serology and semen culture for the diagnosis of brucella ovis infection in chronically infected rams. | the serological response to brucella ovis and the shedding of the organism in semen was followed for a period of 13-14 months in 42 naturally infected rams. most rams remained chronically infected and excreted the organism in their semen throughout the investigation. b. ovis was isolated from 87.9% of the semen samples from the infected rams. the most common sites from which b. ovis could be isolated at necropsy were the epididymides and accessory sexual glands. in one ram the organism was isola ... | 1985 | 16031169 |
| restriction endonuclease (ecor1) analysis of brucella ovis dna. | seven brucella ovis isolates from diverse sources (including two used in the manufacture of a br. ovis vaccine, four field strains and a reference strain from the national culture type collection in london) were subjected to bacterial restriction endonuclease dna analysis using the endonuclease ecor1. no genetic differences were detected. it was concluded that strain variation is unlikely to be a problem in br. ovis control schemes, whether these are based on vaccination or on the eradication of ... | 1987 | 16031329 |
| the serological response of rams to four methods of vaccination against brucella ovis infection. | a comparative study was made of the serological response of rams to an inactivated brucella ovis saline in-oil vaccine administered either once or twice by either the subcutaneous or the intraperitoneal route. the serological response of rams to two spaced doses of vaccine was more consistent and more persistent than when a single dose of vaccine was used. the rise in titre was more rapid and the final titre of greater magnitude when the subcutaneous route of administration was used in compariso ... | 1987 | 16031388 |
| local tissue reaction to the administration of an inactivated brucella ovis saline-in-oil vaccine by the intraperitoneal route. | an inactivated brucella ovis saline-in-oil vaccine was administered to 14 adult ewes using both the intraperitoneal route and the subcutaneous route. pairs of animals were necropsied at intervals between 24 hours and ten weeks after injection. the nature of the local inflammatory reaction to the administration of the vaccine was similar at all sites. the lesion consisted of granulomatous inflammation arranged around droplets of oily vaccine. diffuse peritonitis was seen at necropsy in 12 of the ... | 1988 | 16031427 |
| the response of vaccinated rams to experimental challenge using brucella ovis. | one hundred and thirty eight rams were allocated to four experimental groups. an inactivated br. ovis vaccine was administered either once by the intraperitoneal route (1 i/p), twice by the intraperitoneal route (2 i/p), or twice by the subcutaneous route (2 s/c), and the last group was left unvaccinated. they were then challenged by the intravenous inoculation of between 123 and 1.23 x 108 live br. ovis bacteria. the number of rams that succumbed to infection within the four groups was 4135 (11 ... | 1988 | 16031445 |
| observations on the eradication of brucella ovis infection from a ram flock. | the measures taken to eradicate brucella ovis infection from a naturally infected flock of 64 rams are described. lesions of epididymitis were detected in 18 rams, all of which gave either positive or suspicious reactions in the complement fixation test. a further 20 rams gave serological reactions in the complement fixation test. subsequently, semen was collected from 14 of these 20 rams and b. ovis was cultured from the semen of all 14 rams. serum samples from two rams failed to react in the c ... | 1991 | 16031612 |
| serological and necropsy findings for rams infected with brucella ovis which were not identified by the complement fixation test. | the eradication of brucella ovis from a commercial flock of 36 romney rams was complicated by four infected rams remaining undetected despite four successive flock examinations using the complement fixation test. these four rams were subsequently tested using an enzyme-linked immunosorbent assay and a gel diffusion test and shown to be infected by semen culture. all four rams could have been identified as infected at the initial test if the enzyme-linked immunosorbent assay had been used in addi ... | 1993 | 16031700 |
| use of the double immuno gel diffusion test and the enzyme-linked immunosorbent assay to distinguish false from true reactors in the complement fixation test for brucella ovis. | a gel diffusion test with sonicated brucella ovis antigen and an enzyme-linked immunosorbent assay based on heat-extracted antigen were used to distinguish false from true reactions in a complement fixation test based on heat-extracted antigen. of 142 complement fixing reactors (occurring in supposedly brucella ovis-free, accredited flocks), the gel diffusion test correctly identified the status of 139 animals as compared to 128 with the enzyme-linked immunosorbent assay. a combination of the tw ... | 1993 | 16031707 |
| seroprevalence of brucella ovis infection in commercial ram flocks in the tamworth area. | commercial ram flocks in the tamworth area of northern new south wales were surveyed to estimate the proportion of flocks, and rams, infected with brucella ovis. the flock prevalence (percentage of flocks containing seropositive rams) of 9.1% for merino flocks was significantly lower than that for british-breed flocks (43.8%, p=0.006) and mixed-breed flocks (46.7%, p=0.017). the mean flock prevalence over all flock types was 32.9%. these estimates were supported by data obtained from diagnostic ... | 1994 | 16031755 |
| attempted definition by immunoblotting of the causes of reactivity in suspected false-positive sera in the brucella ovis complement fixation test. | seventy-nine suspected false-positive sera, obtained over 1 year from routine submissions for brucella ovis serological testing, were used in this study. these sera, which exhibited titres in the complement fixation test, but which because of their epidemiological history and their reactions in the enzyme-linked immunosorbent assay and gel diffusion test were suspected to be false positives, were further analysed by immunoblotting. in blots, using b. ovis antigens, rough lipopolysaccharide was i ... | 1996 | 16031926 |
| an improved immunoblotting technique for the serodiagnosis of brucella ovis infections. | two antigen preparations, the routinely used brucella ovis sodium dodecylsulfate-mercapto ethanol extract and a b. ovis triton x-114-derived detergent-rich phase, were compared under standard conditions for their use in electrophoretic immunoblotting for confirmafory, serological testing for b. ovis infections, by using 88 sera from ram flocks with a history of freedom from b. ovis infections, 80 sera from chronically infected rams, which were shedding b. ovis in their semen at the time of sampl ... | 1997 | 16031955 |
| evaluation of electrophoretic immunoblotting for brucella ovis infection in deer using ram and deer serum. | recently the first case of natural infection of deer with brucella ovis was discovered. the aim of this study was to develop and evaluate an electrophoretic immunoblotting method for testing deer serum for specific b. ovis antibodies. | 1998 | 16032007 |
| performance of an enzyme-linked immunosorbent assay for the diagnosis of brucella ovis infection in rams. | to describe the performance characteristics (sensitivity and specificity) of an enzyme-linked immunosorbent assay (elisa) for the diagnosis of brucella ovis infection in rams. | 1999 | 16032074 |
| transmission of brucella ovis from rams to red deer stags. | to determine whether b. ovis will transmit from infected rams to non-infected red deer stags (cervus elaphus) grazing together in the same paddock. | 2000 | 16032119 |
| attempted transmission of brucella ovis between red deer stags by successive grazing or adjacent-paddock grazing. | to determine whether brucella ovis can be transmitted from stag to stag by successive grazing of infected and noninfected stags in the same paddock, or by grazing infected and non-infected stags in adjacent paddocks. | 2000 | 16032138 |
| effects of brucella ovis infection on semen characteristics of 16-month-old red deer stags. | to determine the effects of brucella ovis infection on semen characteristics of 16-month-old red deer stags (cervus elaphus). | 2002 | 16032204 |
| control and eradication of animal diseases in new zealand. | new zealand is free from all the major epidemic (office international des epizooties list a) diseases of animals and other important diseases, such as rabies and the transmissible spongiform encephalopathies. the once endemic conditions of sheep scab (psoroptes ovis), bovine brucellosis (brucella abortus), hydatids (echinococcus granulosus) and aujeszky's disease have been eradicated. anthrax (bacillus anthracis) is no longer considered endemic and pullorum disease (salmonella pullorum) has effe ... | 2002 | 16032229 |
| an overview of brucella ovis infection in new zealand. | | 2002 | 16032250 |
| effects of vaginal brucella ovis infection of red deer hinds on reproductive performance, and venereal transmission to stags. | to investigate the effects of vaginal brucella ovis infection on the reproductive performance of red deer (cervus elaphus) hinds. to determine whether stags may become infected with b. ovis by venereal transmission from mating infected hinds. | 2002 | 16032258 |
| characterization of the genome composition of bartonella koehlerae by microarray comparative genomic hybridization profiling. | bartonella henselae is present in a wide range of wild and domestic feline hosts and causes cat-scratch disease and bacillary angiomatosis in humans. we have estimated here the gene content of bartonella koehlerae, a novel species isolated from cats that was recently identified as an agent of human endocarditis. the investigation was accomplished by comparative genomic hybridization (cgh) to a microarray constructed from the sequenced 1.93-mb genome of b. henselae. control hybridizations of labe ... | 2005 | 16109957 |
| whole-genome analyses of speciation events in pathogenic brucellae. | despite their high dna identity and a proposal to group classical brucella species as biovars of brucella melitensis, the commonly recognized brucella species can be distinguished by distinct biochemical and fatty acid characters, as well as by a marked host range (e.g., brucella suis for swine, b. melitensis for sheep and goats, and brucella abortus for cattle). here we present the genome of b. abortus 2308, the virulent prototype biovar 1 strain, and its comparison to the two other human patho ... | 2005 | 16299333 |
| brucella outer membrane complex-loaded microparticles as a vaccine against brucella ovis in rams. | due to the important drawbacks of the brucella melitensis rev 1 vaccine, a safer vaccine based on an outer membrane complex from brucella ovis encapsulated in poly-epsilon-caprolactone (pec) microparticles (mp) was developed and tested in rams. homogeneous batches of microparticles were prepared by a new double emulsion solvent evaporation method called "total recirculation one-machine system" (troms). such microparticles presented a mean diameter of 2 microm and displayed an antigen loading of ... | 2006 | 16337315 |
| highly sensitive real-time pcr for specific detection and quantification of coxiella burnetii. | coxiella burnetii, the bacterium causing q fever, is an obligate intracellular biosafety level 3 agent. detection and quantification of these bacteria with conventional methods is time consuming and dangerous. during the last years, several pcr based diagnostic assays were developed to detect c. burnetii dna in cell cultures and clinical samples. we developed and evaluated taqman-based real-time pcr assays that targeted the singular icd (isocitrate dehydrogenase) gene and the transposase of the ... | 2006 | 16423303 |
| evaluation of the sprague-dawley rat as a model for vertical transmission of brucella abortus. | vertical transmission of brucella abortus in sprague-dawley (sd) rats was verified with microbiologic, serologic, and polymerase chain reaction (pcr) methods. the 38 initially brucella-free sd rats, weighing 200 to 250 g, were injected subcutaneously with 50 microl of a suspension containing 1 x 10(9) colony-forming units (cfu) of b. abortus biotype 1 korean isolate. the rats were allowed to mate with uninfected sd rats. the isolate was detected by culture and by amos (abortus, melitensis, ovis, ... | 2005 | 16479730 |
| experiments on a sub-unit vaccine encapsulated in microparticles and its efficacy against brucella melitensis in mice. | the aim of this study was to evaluate the effect of the excipients used to facilitate the encapsulation of high hydrophobic antigenic complex extracted from brucella ovis (hs) on the physico-chemical properties of the resulting microparticles. poly(epsilon-caprolactone) (pec) microparticles containing hs were prepared by the solvent extraction/evaporation method using total recirculation one-machine system (troms). different excipients, beta-cyclodextrin (beta-cd), pluronic f68, tween 20 or twee ... | 2006 | 16481077 |
| brucella outer membrane protein omp31 is a haemin-binding protein. | the expression of haemin-binding proteins (hbps) in the outer membrane is one of the strategies used by gram-negative bacteria to obtain iron from the host. no hbp has been described in brucella spp. we investigated whether omp31, an outer membrane protein from brucella with homology to hbps from bartonella quintana, is an hbp. soluble recombinant omp31 bound specifically to haemin-agarose, while an unrelated brucella protein (sura) did not. a similar experiment showed that native omp31 found in ... | 2006 | 16517201 |
| identification of an immunogenic protein of actinobacillus seminis that is present in microvesicles. | actinobacillus seminis is a gram-negative bacterium of the pasteurellaceae family that is involved in ovine epididymitis. looking for a protein specific to this species, we determined the protein profile of subcellular fractions of a. seminis (american type culture collection number 15768): proteins from the outer membrane (omps), inner membrane (imps), and cytoplasm (cps). these profiles provide the first data, to our knowledge, regarding subcellular fractions of a. seminis. in the omp fraction ... | 2006 | 16548331 |
| brucella spp noncanonical lps: structure, biosynthesis, and interaction with host immune system. | brucella spp. are facultative intracellular pathogens that have the ability to survive and multiply in professional and non-professional phagocytes, and cause abortion in domestic animals and undulant fever in humans. several species are recognized within the genus brucella and this classification is mainly based on the difference in pathogenicity and in host preference. brucella strains may occur as either smooth or rough, expressing smooth lps (s-lps) or rough lps (r-lps) as major surface anti ... | 2006 | 16556309 |
| persistence, serodiagnosis and effects on semen characteristics of artificial brucella ovis infection in red deer stags. | to investigate the persistence of infection and serum antibody titres after infection of red deer (cervus elaphus) stags with brucella ovis, and compare these with those of rams. to assess the effects of recent and chronic infection on semen characteristics of stags. | 2006 | 16596160 |
| protective immunity elicited by a divalent dna vaccine encoding both the l7/l12 and omp16 genes of brucella abortus in balb/c mice. | this study was designed to evaluate the immunogenicity and the protective efficacy of a divalent fusion dna vaccine encoding both the brucella abortus l7/l12 protein (ribosomal protein) and omp16 protein (outer membrane lipoprotein), designated pcdna3.1-l7/l12-omp16. intramuscular injection of this divalent dna vaccine into balb/c mice elicited markedly both humoral and cellular immune responses. the specific antibodies exhibited a dominance of immunoglobulin g2a (igg2a) over igg1. in addition, ... | 2006 | 16622210 |
| cghscan: finding variable regions using high-density microarray comparative genomic hybridization data. | comparative genomic hybridization can rapidly identify chromosomal regions that vary between organisms and tissues. this technique has been applied to detecting differences between normal and cancerous tissues in eukaryotes as well as genomic variability in microbial strains and species. the density of oligonucleotide probes available on current microarray platforms is particularly well-suited for comparisons of organisms with smaller genomes like bacteria and yeast where an entire genome can be ... | 2006 | 16638145 |
| rapid detection of brucella spp. in blood cultures by fluorescence in situ hybridization. | brucellosis is a severe systemic disease in humans. we describe a new 16s rrna-based fluorescence in situ hybridization assay that facilitates rapid and specific detection of all human pathogenic species of brucella and that can be applied directly to positive blood cultures. | 2006 | 16672413 |
| detection of antibodies to brucella ovis in sheep milk using b. ovis and b. canis antigen. | the diagnostic techniques most widely used for detecting brucellosis caused by brucella ovis are serological tests such as complement fixation (cft), agar gel immunodiffusion (agid), and elisas. however, to our knowledge, milk tests, with the advantage that samples may be taken in a non invasive manner, have not been investigated as diagnostic tools. we studied 144 samples of milk and sera from lactating ewes, comparing bacteriological studies, serological and milk tests using brucella canis and ... | 2006 | 16678362 |
| ram epididymitis: a clinical report. | two hundred and five ram lambs originating from several sources were assembled for a ram performance trial. all rams were immunized with a commercial brucella ovis bacterin. four rams developed clinical evidence of epididymitis during the 150 day trial. actinobacillus seminis was identified as the causative agent. | 1982 | 16725695 |
| semen characteristics, scrotal circumference and bacterial isolates of fine wool range rams. | during 1983, 887 fine wool rams were subjected to breeding soundness evaluation to determine effects of age and scrotal circumference on semen characteristics. of rams evaluated, 94.4, 84.8, and 81.9% of young (</=1.5 yr), mature (2 to 5 yr), and old (>6 yr), respectively, were rated as satis-factory. old rams had fewer (p < 0.05) motile cells and more (p < 0.05) abnormal cells than young rams. among older rams there was a higher percentage (p < 0.05) with testicular lesions than among young or ... | 1987 | 16726345 |
| relationships between brucella ovis semen culture and various semen and serology parameters. | semen and blood samples from 154 rams from two montana range flocks (flock a, vaccinated for brucella ovis ; flock b, nonvaccinated) were evaluated to determine the relationship between brucella ovis (b. ovis ) semen culture results and various semen and blood parameters. all rams utilized in this study exhibited no palpable ram epididymitis lesions. thirteen and 25.6% of the rams tested in flocks a and b, respectively, had positive b. ovis semen cultures. only age of ram and ram condition score ... | 1988 | 16726417 |
| adherence of brucella ovis to preimplantation ovine ova. | seventy-three zona pellucida-intact ova were collected surgically from 15 superovulated, brucella -free mixed-breed ewes. twenty-one groups containing one to five ova were incubated in medium containing brucella ovis . subsequently, seven and five groups were incubated for 24 and 4 h, respectively, at 37 degrees c in medium containing penicillin and streptomycin, while nine groups were not treated with antibiotics. all groups of ova were washed 10 times, and ova and sequential washes were cultur ... | 1988 | 16726479 |
| seroconversion of recipient ewes after transfer of ova exposed to brucella ovis in vitro. | zona pellucida-intact ova collected from ewes seronegative to brucella ovis were exposed in vitro to b ovis and washed 10 times in medium that contained no antibiotics. after exposure and washing, nontransferable ova were cultured for isolation of brucella , and the viable ova were transferred into seven b ovis seronegative ewes. no pregnancies resulted, thus recipient ewes were bred during the next breeding season, and blood samples were collected for bacteriological and serological examination ... | 1990 | 16726896 |
| optimization of the entrapment of bacterial cell envelope extracts into microparticles for vaccine delivery. | the encapsulation of a brucella ovis extract (hs) in microparticles has been proved effective against experimental infections in mice. this work describes different strategies to increase the hs loading and prepare large batches as necessary to test this vaccine in ovine. the mixture of hs with beta-cyclodextrin was optimized in order to increase the hs loading in microparticles. on the other hand, troms ('total recirculation one-machine system') led microparticles with a more homogeneous size t ... | 2006 | 16754373 |
| evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative rods. | to evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ... | 2007 | 16822636 |
| cloning, characterization, and expression of bartonella henselae p26. | in order to identify immunoreactive bartonella henselae proteins, b. henselae antiserum from an experimentally infected cat was used to screen a b. henselae genomic dna expression library. one immunoreactive phage clone contained a gene (p26) with significant nucleotide identity with orthologs in brucellae, bartonellae, and several plant-associated bacteria. p26 gene sequences from four b. henselae strains, one b. koehlerae strain, and one b. clarridgeiae strain were cloned. comparative nucleoti ... | 2006 | 16893981 |
| intrinsic and selected resistance to antibiotics binding the ribosome: analyses of brucella 23s rrn, l4, l22, ef-tu1, ef-tu2, efflux and phylogenetic implications. | brucella spp. are highly similar, having identical 16s rna. however, they have important phenotypic differences such as differential susceptibility to antibiotics binding the ribosome. neither the differential susceptibility nor its basis has been rigorously studied. differences found among other conserved ribosomal loci could further define the relationships among the classical brucella spp. | 2006 | 17014718 |
| multiplex pcr for the detection of brucella ovis, actinobacillus seminis and histophilus somni in ram semen. | to develop a multiplex polymerase chain reaction (pcr) assay for the rapid detection of brucella ovis, actinobacillus seminis, histophilus somni in fresh ram semen samples. | 2007 | 17300467 |
| epididymitis caused by brucella ovis in a southern ontario sheep flock. | epididymitis was diagnosed in three rams in a commercial sheep flock in southern ontario. the affected rams had palpably enlarged epididymides and two rams had semen which contained inflammatory cells and was of poor quality. serum compliment fixation titers for brucella ovis were 1:20, 1:80 and 1:90. five other rams in the flock were clinically normal and without titers. two of the affected rams had lesions similar to those produced by experimental infection with b. ovis. the infection in the r ... | 1985 | 17422577 |
| ovine brucellosis in alberta. | two parallel surveys of rams from alberta sheep flocks were conducted to determine the presence of infection with brucella ovis. in a retrospective study over a period of 24 months, using complement fixation test, 12 flocks out of 142 tested were considered infected. in another 17-month survey of slaughter rams by serology and culture methods 11 flocks out of 124 were found to be infected. the overall prevalence of ovine brucellosis was 8.6% of the flocks tested which represented 12.5% of the es ... | 1986 | 17422669 |
| ontario. brucella ovis, une cause d'avortement chez le mouton. | | 1991 | 17423724 |
| improved immunogenicity of a vaccination regimen combining a dna vaccine encoding brucella melitensis outer membrane protein 31 (omp31) and recombinant omp31 boosting. | in the present study, we report an attempt to improve the immunogenicity of the omp31 antigen by a dna prime-protein boost immunization regimen. we immunized balb/c mice with an omp31 dna vaccine (pciomp31) followed by boosting with recombinant omp31 (romp31) in incomplete freund's adjuvant and characterized the resulting immune responses and the protective efficacy against brucella ovis and b. melitensis infection. immunoglobulin g1 (igg1) and igg2a titers were higher in sera from pciomp31/romp ... | 2007 | 17428946 |
| a recombinant subunit vaccine based on the insertion of 27 amino acids from omp31 to the n-terminus of bls induced a similar degree of protection against b. ovis than rev.1 vaccination. | the development of an effective subunit vaccine against brucellosis is a research area of intense interest. the enzyme lumazine synthase from brucella spp. (bls) is highly immunogenic, presumably due to its decameric arrangement and remarkable stability. in this work we decided to develop a chimera with the scaffold protein bls decorated with 10 copies of a known protective epitope derived from an outer membrane protein of 31kda (omp31) from brucella spp. vaccination of balb/c mice with the chim ... | 2007 | 17442465 |
| characterisation of the genetic diversity of brucella by multilocus sequencing. | brucella species include economically important zoonotic pathogens that can infect a wide range of animals. there are currently six classically recognised species of brucella although, as yet unnamed, isolates from various marine mammal species have been reported. in order to investigate genetic relationships within the group and identify potential diagnostic markers we have sequenced multiple genetic loci from a large sample of brucella isolates representing the known diversity of the genus. | 2007 | 17448232 |
| brucella isolated in humans and animals in latin america from 1968 to 2006. | we report a retrospective analysis of 1933 brucella strains isolated from humans and animals in latin american countries between 1968 and 1991 and in argentina between 1994 and 2006. during the first period 50% of strains were from humans, mainly from argentina, mexico and peru but, while b. suis was the main cause of infection in argentina, b. melitensis was responsible for most infections in the other countries. in argentina in the later years, b. melitensis and b. suis were observed more freq ... | 2008 | 17559694 |
| brucella isolated in humans and animals in latin america from 1968 to 2006. | we report a retrospective analysis of 1933 brucella strains isolated from humans and animals in latin american countries between 1968 and 1991 and in argentina between 1994 and 2006. during the first period 50% of strains were from humans, mainly from argentina, mexico and peru but, while b. suis was the main cause of infection in argentina, b. melitensis was responsible for most infections in the other countries. in argentina in the later years, b. melitensis and b. suis were observed more freq ... | 2008 | 17559694 |
| role of the omp25/omp31 family in outer membrane properties and virulence of brucella ovis. | the genes coding for the five outer membrane proteins (omps) of the omp25/omp31 family expected to be located in the outer membrane (om) of rough virulent brucella ovis pa were inactivated to evaluate their role in virulence and om properties. the om properties of the mutant strains and of the mutants complemented with the corresponding wild-type genes were analyzed, in comparison with the parental strain and rough b. abortus rb51, in several tests: (i) binding of anti-omp25 and anti-omp31 monoc ... | 2007 | 17562767 |
| gantrez an nanoparticles as an adjuvant for oral immunotherapy with allergens. | the aim of this study was to investigate the adjuvant properties of oral-administered gantrez an nanoparticles with ovalbumin (as allergen model) and, in some cases, lipopolysaccharide of brucella ovis as immunomodulator. for this purpose, balb/c mice were administered by oral gavage with ova nanoparticles and both th1 and th2 markers (igg2a and igg1, respectively) were enhanced. on the other hand, these carriers administered by oral route were able to protect a model of sensitized mice to ovalb ... | 2007 | 17576025 |
| a dna vaccine coding for the chimera blsomp31 induced a better degree of protection against b. ovis and a similar degree of protection against b. melitensis than rev.1 vaccination. | in the present study, we reported an attempt to improve the immunogenicity and protective capacity of the chimera blsomp31 using a different antigen delivery: dna vaccination. vaccination of balb/c mice with the dna vaccine coding for the chimera blsomp31 (pciblsomp31) provided the best protection level against brucella ovis, which was significantly higher than the given by the co-delivery of both plasmids coding for the whole proteins (pcdnabls+pciomp31) and even higher than the control vaccine ... | 2007 | 17600596 |
| detection of ovine antibody to brucella ovis by indirect enzyme immunoassay. | because some batch-to-batch variation in the preparation of rough lipopolysaccharide (rlps) from brucella ovis has been experienced, several protocols were tested to establish the most reliable method for detection of antibody in indirect enzyme immunoassay. an early version of the assay gave a performance index (pi=sum of optimum percent sensitivity and percent specificity, determined by receiver operator characteristic analysis) of 198.6. this assay used rlps from b. ovis as the antigen and a ... | 2007 | 17613670 |
| brucella abortus uses a stealthy strategy to avoid activation of the innate immune system during the onset of infection. | to unravel the strategy by which brucella abortus establishes chronic infections, we explored its early interaction with innate immunity. | 2007 | 17637846 |
| bvrr/bvrs-controlled outer membrane proteins omp3a and omp3b are not essential for brucella abortus virulence. | the brucella abortus two-component regulatory system bvrr/bvrs controls the expression of outer membrane proteins (omp) omp3a (omp25) and omp3b (omp22). disruption of bvrs or bvrr generates avirulent mutants with altered cell permeability, higher sensitivity to microbicidal peptides, and complement. consequently, the role of omp3a and omp3b in virulence was examined. similar to bvrs or bvrr mutants, omp3a and omp3b mutants displayed increased attachment to cells, indicating surface alterations. ... | 2007 | 17664262 |
| novel brucella strain (bo1) associated with a prosthetic breast implant infection. | we report the microbiological, biochemical, and molecular characterization of an unusual brucella strain (bo1) isolated from a breast implant wound in a 71-year-old woman with clinical symptoms consistent with brucellosis. initial phenotypic analysis, including biochemical and antimicrobial susceptibility testing, cellular fatty acid analysis, and molecular analysis based on dna-dna reassociation and the presence of multiple copies of is711 element suggested that the isolate was a brucella-like ... | 2008 | 17977982 |
| novel brucella strain (bo1) associated with a prosthetic breast implant infection. | we report the microbiological, biochemical, and molecular characterization of an unusual brucella strain (bo1) isolated from a breast implant wound in a 71-year-old woman with clinical symptoms consistent with brucellosis. initial phenotypic analysis, including biochemical and antimicrobial susceptibility testing, cellular fatty acid analysis, and molecular analysis based on dna-dna reassociation and the presence of multiple copies of is711 element suggested that the isolate was a brucella-like ... | 2008 | 17977982 |
| phylogenomics and signature proteins for the alpha proteobacteria and its main groups. | alpha proteobacteria are one of the largest and most extensively studied groups within bacteria. however, for these bacteria as a whole and for all of its major subgroups (viz. rhizobiales, rhodobacterales, rhodospirillales, rickettsiales, sphingomonadales and caulobacterales), very few or no distinctive molecular or biochemical characteristics are known. | 2007 | 18045498 |
| demonstration of is711 transposition in brucella ovis and brucella pinnipedialis. | the brucella genome contains an insertion sequence (is) element called is711 or is6501, which is specific to the genus. the copy number of is711 varies in the genome of the different brucella species, ranging from 7 in b. abortus, b. melitensis and b. suis to more than 30 in b. ovis and in brucella strains isolated from marine mammals. at present, there is no experimental evidence of transposition of is711, but the occurrence of this element with a high copy number in some species, and the isola ... | 2008 | 18218072 |
| brucella microti sp. nov., isolated from the common vole microtus arvalis. | two gram-negative, non-motile, non-spore-forming, coccoid bacteria (strains ccm 4915(t) and ccm 4916), isolated from clinical specimens of the common vole microtus arvalis during an epizootic in the czech republic in 2001, were subjected to a polyphasic taxonomic study. on the basis of 16s rrna (rrs) and reca gene sequence similarities, both isolates were allocated to the genus brucella. affiliation to brucella was confirmed by dna-dna hybridization studies. both strains reacted equally with bru ... | 2008 | 18218934 |
| putative quorum-sensing regulator blxr of brucella melitensis regulates virulence factors including the type iv secretion system and flagella. | brucella melitensis is an intracellular pathogen that establishes a replicative niche within macrophages. while the intracellular lifestyle of brucella is poorly understood and few virulence factors have been identified, components of a quorum-sensing pathway in brucella have recently been identified. the luxr-type regulatory protein, vjbr, and an n-acylhomoserine lactone signaling molecule are both involved in regulating expression of the virb-encoded type iv secretion system. we have identifie ... | 2008 | 18310341 |
| brucella melitensis b115-based complement fixation test to detect antibodies induced by brucella rough strains. | to assess the efficiency of a brucella melitensis b115 rough strain, naturally devoid of anticomplementary activity, used as antigen in a complement fixation test (cft) to detect antibodies induced by brucella strains with rough phenotype, such as brucella abortus rb51, brucella ovis and brucella canis. | 2008 | 18355234 |
| rapid polymerase chain reaction-based screening assay for bacterial biothreat agents. | to design and evaluate a rapid polymerase chain reaction (pcr)-based assay for detecting eubacteria and performing early screening for selected class a biothreat bacterial pathogens. | 2008 | 18370996 |
| cghsweep: an algorithm for analyzing chromosomal aberrations in genome using acgh profiles. | dna copy number aberrations along the genome are vital markers for studying pathogenesis of various diseases including cancers. array-based comparative genome hybridization (acgh), which is a high-throughput cytogenetic method, helps in identifying genome-wide copy number aberrations, both gains and losses. here, we propose a computational technique to analyze acgh data and to identify potential dna copy number alterations along the genome. our technique detects the possible breakpoints by compa ... | 2007 | 18467776 |
| rapid identification of brucella isolates to the species level by real time pcr based single nucleotide polymorphism (snp) analysis. | brucellosis, caused by members of the genus brucella, remains one of the world's major zoonotic diseases. six species have classically been recognised within the family brucella largely based on a combination of classical microbiology and host specificity, although more recently additional isolations of novel brucella have been reported from various marine mammals and voles. classical identification to species level is based on a biotyping approach that is lengthy, requires extensive and hazardo ... | 2008 | 18518958 |
| allergen immunotherapy with nanoparticles containing lipopolysaccharide from brucella ovis. | the adjuvant and protective capacity against anaphylactic shock of the association between rough lipopolysaccharide of brucella ovis (lps) coencapsulated with ovalbumin (ova), as a model allergen, in gantrez an nanoparticles was investigated. several strategies were performed in order to study the adjuvant effect of the lps either encapsulated or coating the nanoparticles. ova, as well as lps, was incorporated either during the manufacturing process (ova-encapsulated or lps-encapsulated nanopart ... | 2008 | 18582571 |
| a bioinformatic approach to understanding antibiotic resistance in intracellular bacteria through whole genome analysis. | intracellular bacteria survive within eukaryotic host cells and are difficult to kill with certain antibiotics. as a result, antibiotic resistance in intracellular bacteria is becoming commonplace in healthcare institutions. owing to the lack of methods available for transforming these bacteria, we evaluated the mechanisms of resistance using molecular methods and in silico genome analysis. the objective of this review was to understand the molecular mechanisms of antibiotic resistance through i ... | 2008 | 18619818 |
| brucellosis vaccines: assessment of brucella melitensis lipopolysaccharide rough mutants defective in core and o-polysaccharide synthesis and export. | the brucellae are facultative intracellular bacteria that cause brucellosis, one of the major neglected zoonoses. in endemic areas, vaccination is the only effective way to control this disease. brucella melitensis rev 1 is a vaccine effective against the brucellosis of sheep and goat caused by b. melitensis, the commonest source of human infection. however, rev 1 carries a smooth lipopolysaccharide with an o-polysaccharide that elicits antibodies interfering in serodiagnosis, a major problem in ... | 2008 | 18648644 |
| rnai screen of endoplasmic reticulum-associated host factors reveals a role for ire1alpha in supporting brucella replication. | brucella species are facultative intracellular bacterial pathogens that cause brucellosis, a global zoonosis of profound importance. although recent studies have demonstrated that brucella spp. replicate within an intracellular compartment that contains endoplasmic reticulum (er) resident proteins, the molecular mechanisms by which the pathogen secures this replicative niche remain obscure. here, we address this issue by exploiting drosophila s2 cells and rna interference (rnai) technology to de ... | 2008 | 18654626 |
| prediction of sinorhizobium meliloti srna genes and experimental detection in strain 2011. | small non-coding rnas (srnas) have emerged as ubiquitous regulatory elements in bacteria and other life domains. however, few srnas have been identified outside several well-studied species of gamma-proteobacteria and thus relatively little is known about the role of rna-mediated regulation in most other bacterial genera. here we have conducted a computational prediction of putative srna genes in intergenic regions (igrs) of the symbiotic alpha-proteobacterium s. meliloti 1021 and experimentally ... | 2008 | 18793445 |
| incidence of brucella species in marine mammals of the german north sea. | in this study, organ samples from 426 common seals phoca vitulina, 298 harbour porpoises phocoena phocoena, 34 grey seals halichoerus grypus and 10 other marine mammals were assessed for the presence of brucella species. forty-seven common seals, 2 harbour porpoises and 1 grey seal were found to be positive for these bacteria. a total of 91 brucella strains were successfully isolated, due to the fact that brucella spp. were found in more than one organ sample in 15 animals. the primary organ in ... | 2008 | 18828563 |
| stability of poly(epsilon-caprolactone) microparticles containing brucella ovis antigens as a vaccine delivery system against brucellosis. | in previous works, our research group has successfully proved the use of subcellular vaccines based on poly(epsilon-caprolactone) (pec) microparticles containing an antigenic extract of brucella ovis (hs) against experimental brucellosis in both mice and rams. however, the successful exploitation of pharmaceutical products, and therefore of this product as veterinary vaccine, requires preservation of both biological activity and native structure in all steps of development from purification to s ... | 2008 | 18923907 |
| evaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against brucella abortus srb51 in vaccinated heifers. | the live attenuated brucella abortus srb51 (srb51) is a partial o-chain-deprived mutant. the relative lack of the polysaccharide prevents it from inducing antibodies detectable by most of the serological tests used for the diagnosis of bovine brucellosis. the performance of three antigens used in an indirect enzyme-linked immunoassay test for detecting srb51 antibodies were evaluated. a homogeneous group of twenty-five 10-month-old hereford heifers was used. the animals were bled on day 0 and th ... | 2009 | 18980780 |
| immunization with recombinant brucella species outer membrane protein omp16 or omp19 in adjuvant induces specific cd4+ and cd8+ t cells as well as systemic and oral protection against brucella abortus infection. | available vaccines against brucella spp. are live attenuated brucella strains. in order to engineer a better vaccine to be used in animals and humans, our laboratory aims to develop an innocuous subunit vaccine. particularly, we are interested in the outer membrane proteins (omps) of b. abortus: omp16 and omp19. in this study, we assessed the use of these proteins as vaccines against brucella in balb/c mice. immunization with lipidated omp16 (l-omp16) or l-omp19 in incomplete freund's adjuvant ( ... | 2009 | 18981242 |
| immunization with recombinant brucella species outer membrane protein omp16 or omp19 in adjuvant induces specific cd4+ and cd8+ t cells as well as systemic and oral protection against brucella abortus infection. | available vaccines against brucella spp. are live attenuated brucella strains. in order to engineer a better vaccine to be used in animals and humans, our laboratory aims to develop an innocuous subunit vaccine. particularly, we are interested in the outer membrane proteins (omps) of b. abortus: omp16 and omp19. in this study, we assessed the use of these proteins as vaccines against brucella in balb/c mice. immunization with lipidated omp16 (l-omp16) or l-omp19 in incomplete freund's adjuvant ( ... | 2009 | 18981242 |
| efficacy of bp26 and bp26/omp31 b. melitensis rev.1 deletion mutants against brucella ovis in rams. | brucella melitensis rev.1 is the most effective vaccine against b. ovis infection in sheep but induces antibodies interfering with b. melitensis diagnosis. brucella bp26 and omp31 proteins are differential diagnostic antigens. single or double bp26 and omp31 rev.1 deletion mutants have been proven effective against b. melitensis in sheep. here, the cgv26 (deleted in bp26 gene) and cgv2631 (deleted in both bp26 and omp31 genes) mutants have been tested for efficacy against b. ovis in rams. either ... | 2009 | 19007836 |
| differential expression of inflammatory and immune response genes in rams experimentally infected with a rough virulent strain of brucella ovis. | infection of sheep with brucella ovis results in ovine brucellosis, a disease characterized by infertility in rams, abortion in ewes and increased perinatal mortality in lambs. during the course of the infection both the ovine immune response and host cell gene expression are modified. the objective of this research was to conduct a preliminary characterization of differential gene expression in rams experimentally infected with b. ovis by microarray hybridization and real-time rt-pcr. of the 60 ... | 2009 | 19056128 |
| improved prediction of malaria degradomes by supervised learning with svm and profile kernel. | the spread of drug resistance through malaria parasite populations calls for the development of new therapeutic strategies. however, the seemingly promising genomics-driven target identification paradigm is hampered by the weak annotation coverage. to identify potentially important yet uncharacterized proteins, we apply support vector machines using profile kernels, a supervised discriminative machine learning technique for remote homology detection, as a complement to the traditional alignment ... | 2009 | 19057851 |
| improved prediction of malaria degradomes by supervised learning with svm and profile kernel. | the spread of drug resistance through malaria parasite populations calls for the development of new therapeutic strategies. however, the seemingly promising genomics-driven target identification paradigm is hampered by the weak annotation coverage. to identify potentially important yet uncharacterized proteins, we apply support vector machines using profile kernels, a supervised discriminative machine learning technique for remote homology detection, as a complement to the traditional alignment ... | 2009 | 19057851 |
| phenotypic and molecular characterisation of brucella isolates from marine mammals. | bacteria of the genus brucella are the causative organisms of brucellosis in animals and man. previous characterisation of brucella strains originating from marine mammals showed them to be distinct from the terrestrial species and likely to comprise one or more new taxa. recently two new species comprising brucella isolates from marine mammals, b. pinnipedialis and b. ceti, were validly published. here we report on an extensive study of the molecular and phenotypic characteristics of marine mam ... | 2008 | 19091076 |
| a novel brucella isolate in association with two cases of stillbirth in non-human primates - first report. | brucellosis is veterinary and human health problem. | 2009 | 19187435 |
| brucella tir domain-containing protein mimics properties of the toll-like receptor adaptor protein tirap. | toll-like receptors (tlrs) play essential roles in the activation of innate immune responses against microbial infections. tlrs and downstream adaptor molecules contain a conserved cytoplasmic tir domain. tirap is a tir domain-containing adaptor protein that recruits the signaling adaptor myd88 to a subset of tlrs. many pathogenic microorganisms subvert tlr signaling pathways to suppress host immune responses to benefit their survival and persistence. brucella encodes a tir domain-containing pro ... | 2009 | 19196716 |
| real-time pcr for identification of brucella spp.: a comparative study of is711, bcsp31 and per target genes. | culture is considered as the reference standard assay for diagnosis of brucella spp. in humans and animals but it is time-consuming and hazardous. in this study, we evaluated the performances of newly designed real-time pcr assays using taqman probes and targeting the 3 following specific genes: (i) the insertion sequence is711, (ii) bcsp31 and (iii) per genes for the detection of brucella at genus level. the real-time pcr assays were compared to previously described conventional pcr assays targ ... | 2009 | 19200666 |
| intracellular adaptation of brucella abortus. | macrophages were infected with virulent brucella abortus strain 2308 or attenuated strain 19. intracellular bacteria were recovered at different times after infection and their proteomes compared. the virulent strain initially reduced most biosynthesis and altered its respiration; adaptations reversed later in infection. the attenuated strain was unable to match the magnitude of the virulent strain's adjustments. the results provide insight into mechanisms utilized by brucella to establish intra ... | 2009 | 19216536 |
| rapid differential diagnosis between extrapulmonary tuberculosis and focal complications of brucellosis using a multiplex real-time pcr assay. | arduous to differ clinically, extrapulmonary tuberculosis and focal complications of brucellosis remain important causes of morbidity and mortality in many countries. we developed and applied a multiplex real-time pcr assay (m rt-pcr) for the simultaneous detection of mycobacterium tuberculosis complex and brucella spp. | 2009 | 19225565 |
| analysis of ten brucella genomes reveals evidence for horizontal gene transfer despite a preferred intracellular lifestyle. | the facultative intracellular bacterial pathogen brucella infects a wide range of warm-blooded land and marine vertebrates and causes brucellosis. currently, there are nine recognized brucella species based on host preferences and phenotypic differences. the availability of 10 different genomes consisting of two chromosomes and representing six of the species allowed for a detailed comparison among themselves and relatives in the order rhizobiales. phylogenomic analysis of ortholog families show ... | 2009 | 19346311 |
| lab on a chip genotyping for brucella spp. based on 15-loci multi locus vntr analysis. | brucellosis is an important zoonosis caused by the genus brucella. in addition brucella represents potential biological warfare agents due to the high contagious rates for humans and animals. therefore, the strain typing epidemiological tool may be crucial for tracing back source of infection in outbreaks and discriminating naturally occurring outbreaks versus bioterroristic event. a multiple locus variable-number tandem repeats (vntr) analysis (mlva) assay based on 15 polymorphic markers was pr ... | 2009 | 19351390 |
| comparative proteomics analyses reveal the virb of b. melitensis affects expression of intracellular survival related proteins. | brucella melitensis is a facultative, intracellular, pathogenic bacterium that replicates within macrophages. the type iv secretion system encoded by the virb operon (virb) is involved in brucella intracellular survival. however, the underlying molecular mechanisms, especially the target proteins affected by the virb, remain largely unclear. | 2009 | 19401764 |
| characterization of possible correlates of protective response against brucella ovis infection in rams immunized with the b. melitensis rev 1 vaccine. | vaccination with the live attenuated brucella melitensis rev 1 vaccine is used to control ovine brucellosis caused by brucella ovis in sheep. the objective of this study was to identify possible correlates of protective response to b. ovis infection through the characterization by microarray hybridization and real-time rt-pcr of inflammatory and immune response genes differentially expressed in rams previously immunized with b. melitensis rev 1 and experimentally challenged with b. ovis. gene ex ... | 2009 | 19428917 |
| pathogenicity of different strains of histophilus somni in the experimental induction of ovine epididymitis. | the purpose of this study was to determine any differences in pathogenicity when sheep are experimentally infected with different histophilus somni isolates: a) 2336 bovine origin strain; b) an isolate from ram orchitis and epididymitis; c) an isolate from the brain of a sheep with neurological signs; d) an isolate from the vagina of a clinically healthy ewe. a total of 20 rams divided in groups of 5 animals each were inoculated in the epididymis with 1 x 10(7) cfu/ml of h. somni; a negative con ... | 2009 | 19436586 |
| genome degradation in brucella ovis corresponds with narrowing of its host range and tissue tropism. | brucella ovis is a veterinary pathogen associated with epididymitis in sheep. despite its genetic similarity to the zoonotic pathogens b. abortus, b. melitensis and b. suis, b. ovis does not cause zoonotic disease. genomic analysis of the type strain atcc25840 revealed a high percentage of pseudogenes and increased numbers of transposable elements compared to the zoonotic brucella species, suggesting that genome degradation has occurred concomitant with narrowing of the host range of b. ovis. th ... | 2009 | 19436743 |
| design and influence of gamma-irradiation on the biopharmaceutical properties of nanoparticles containing an antigenic complex from brucella ovis. | despite vaccination campaigns, brucellosis is still one of the most common bacterial zoonosis in the world. this work describes the development of a novel formulation strategy to the delivery of the brucella ovis antigenic extract (hs) into ovine mucosal surfaces. thus, hs was entrapped in conventional and mannosylated poly(anhydride) nanoparticles by the solvent displacement method, and the resulting nanosystems were gamma-irradiated to accomplish the sterilization required for the ophthalmic a ... | 2009 | 19442721 |
| first evidence of brucella ovis infection in republic of croatia. | we researched the spread of brucella ovis (b. ovis) infection in sheep during 2002 and 2003 in croatia. a total of 30,635 sheep blood samples were examined using the enzyme-linked immunosorbent assay (elisa). in 2002, 1014 out of 14,404 examined sheep blood samples (7%) from six counties gave positive reactions while 2060 (14.3%) were found suspicious. in 2003, 638 out of 16,221 examined sheep blood samples in nine counties (3.9%) tested positive while 1083 (6.7%) were suspicious. in rams and sh ... | 2009 | 19537042 |
| a novel nanoparticulate adjuvant for immunotherapy with lolium perenne. | specific immunotherapy implies certain drawbacks which could be minimized by the use of appropriate adjuvants, capable of amplifying the right immune response with minimal side effects. in this context, previous studies of our group have demonstrated the adjuvant capacity of gantrez an nanoparticles, which can effectively enhance the immune response. in this work, two types of nanoparticles (with and without lps of brucella ovis as immunomodulator) with encapsulated lolium perenne extract are te ... | 2009 | 19545572 |
| rapid real-time pcr assays for detection of klebsiella pneumoniae with the rmpa or maga genes associated with the hypermucoviscosity phenotype: screening of nonhuman primates. | the relationship of mucoviscosity-associated (maga) and/or regulator of mucoid phenotype (rmpa) genes to the klebsiella pneumoniae hypermucoviscosity (hmv) phenotype has been reported. we previously demonstrated that rmpa+ k. pneumoniae can cause serious disease in african green monkeys and isolated rmpa+ and maga+ hmv k. pneumoniae from other species of non-human primates. to rapidly screen african green monkeys/non-human primates for these infections, we developed three real-time pcr assays. t ... | 2009 | 19644019 |
| brucella microti: the genome sequence of an emerging pathogen. | using a combination of pyrosequencing and conventional sanger sequencing, the complete genome sequence of the recently described novel brucella species, brucella microti, was determined. b. microti is a member of the genus brucella within the alphaproteobacteria, which consists of medically important highly pathogenic facultative intracellular bacteria. in contrast to all other brucella species, b. microti is a fast growing and biochemically very active microorganism with a phenotype more simila ... | 2009 | 19653890 |