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a competitive enzyme immunoassay for the detection of bovine antibodies to brucella abortus using monoclonal antibodies.a competitive enzyme immunoassay (eia) for the detection of circulating bovine antibodies to brucella abortus has been developed using horseradish peroxidase conjugated monoclonal antibodies (mab) raised against b. abortus cell surface antigens. antibodies present in the serum of either vaccinated or infected cattle can apparently displace the conjugated mab from the lipopolysaccharide antigen (lps) in a quantitatively different manner allowing an assessment of immune status of the animal. the r ...19853922105
inhibition of phagosome-lysosome fusion in macrophages by soluble extracts of virulent brucella abortus.water extracts of virulent brucella abortus were able to inhibit phagosome-lysosome fusion in unelicited murine peritoneal macrophages following ingestion of yeast. extracts from an avirulent strain were unable to produce a similar effect. lipopolysaccharide from b abortus did not appear to be involved with the ability of the extracts to inhibit fusion.19853922258
brucella abortus in wildlife on selected cattle farms in alabama.two studies of brucellosis in wildlife on farms where the brucellosis infection prevalence in cattle was known are reported. on a research farm, 233 feral animals of 22 mammalian species and 12 of seven avian species were trapped during three time periods. sixty were studied before cattle were introduced, 128 were studied while 501 cattle infected with brucella abortus were calving and aborting, and 60 specimens were collected 20 mo after the last infected cow calved. selected tissues from 229 w ...19853923213
reduction of non-specific reactions to the brucella abortus serum agglutination test by the addition of edta.serum samples taken from cattle known to be infected with brucella abortus, and samples routinely collected as part of the brucellosis eradication scheme were tested by the serum agglutination test (sat), complement fixation test (cft) and the sat modified by the addition of ethylene diamine tetra-acetic acid (edta). in 64 per cent of the samples giving an sat titre greater than 100 iu, but a cft titre 8.3 icftu or less, the agglutination reaction was sufficiently affected by the action of edta ...19853925508
specific antibody-containing cells in the mammary gland of non-lactating sheep after intraperitoneal and intramammary immunisation.ewes were immunised intraperitoneally with ovalbumin and brucella abortus in freund's complete adjuvant, followed seven days later by intramammary immunisation in which ovalbumin was presented to one mammary gland and brucella abortus to the other. mammary tissue taken after a further seven days contained more antigen-specific plasma cells than ewes given intraperitoneal or intramammary immunisation alone. these cells were found predominantly in the specifically immunised gland and only a few we ...19853925509
non-specific reactions to the brucella abortus sat. 19853925612
delayed-type hypersensitivity responses induced by bovine colostral components.transfer factor-type substances obtained from leukocytic cells and fluid portions of bovine colostrum caused effective passive transfer of delayed-type hypersensitivity responses across species barriers. passive transfer of brucella abortus sensitivity was obtained with equal regularity when using components derived from peripheral blood and colostrum of dams sensitized at 3 and 9 months of age. colostral feedings to calves caused the passive transfer of delayed-type hypersensitivity as early as ...19853925823
characterization of brucella abortus strain 19 isolated from human and bovine tissues and fluids.one hundred isolates of brucella abortus, which were recovered from bovine and human tissues or fluids, were identified as strain 19 by conventional bacteriologic methods. each isolate was examined using a warburg respirometer to determine oxidative rates on substrates of d- and l-alanine, l-glutamic acid, d(+)-galactose, d-ribose, and i-erythritol. these results were compared with those of repository (seed) cultures of strain 19 used for making antigens and vaccines. except on the substrate of ...19853925824
comparison of enzyme-linked immunosorbent assay and complement fixation test for the detection of specific antibody in cattle vaccinated and challenged with brucella abortus.an enzyme-linked immunosorbent assay (elisa) and a complement fixation test (cft) were applied to sera collected regularly from 60 cattle challenged with brucella abortus strain 544. of the 60 cattle, 48 were vaccinated with either b. abortus strain 19 or b. abortus strain 45/20 as calves or adults. the remaining 12 cattle were not vaccinated. of the 27 sera from cattle found to be infected at slaughter, 9 showed aberrant reactions to the cft in that a positive titer after challenge was delayed ...19853926817
profiles of serological reactions following adult cow inoculation with standard dose brucella abortus strain 19 vaccine.experimental use of the standard dose (4-12 x 10(10) viable organisms per dose) brucella abortus strain 19 vaccine (s19) in adult cattle led to a serological response in the complement fixation test (cft) within 2 weeks. the antibody titres recorded could not be distinguished from those arising from infection for a period of 6-11 months. this was in the absence of the booster effect of brucella antigen from either field or vaccine strains. the illegal or unintentional use of s19 in adult cattle ...19853927001
brucella abortus infection in the bank vole (clethrionomys glareolus). 19853928085
cytotoxic antibody dependent cells in mice experimentally infected with brucella abortus.in this study we investigated the cytotoxic activity mediated by antibody dependent cells (k cell) in c57bl/6 mice experimentally infected with brucella abortus. the involvement of the k cells in experimental infection from brucella was studied by evaluating the bacteriolysis in the presence of specific antibodies. after 3 h of incubation, approximately 30 +/- 3% of the labeled brucellae undergo lysis, whereas the presence of lymphocytic populations alone, or of a specific decomplemented serum, ...19853929026
the role of brucella abortus in spontaneous abortion among the black population.a prospective study on brucellosis in 125 black patients presenting with inevitable or incomplete spontaneous abortion not obviously due to another cause is presented. they were all consecutive cases presenting during weekdays to edendale hospital, pietermaritzburg. brucellosis was investigated by means of the history and serological and culture studies of intra-uterine swabs. no case of acute brucella infection was demonstrated; however, there were 5 cases in which the serological finding was c ...19853929401
a "dipstick" enzyme immunoassay for detection of antibody to brucella abortus in cattle sera.an enzyme immunoassay that utilizes antigen bound to a matrix which can be removed from the substrate to stop development is described. the assay which is performed in glass or plastic disposable tubes uses gel-bond film strips for attachment of antigen. the only equipment requirements are a rotary shaker and a spectrophotometer (optional). the antigen coated strips are passed through a series of tubes containing test serum, wash solution, antibody-enzyme conjugate, wash solution and substrate-c ...19853930057
cleavage of bovine immunoglobulin g1 in whey by an extracellular material from brucella abortus.culture extracts of in vitro grown brucella abortus were demonstrated to cleave a part of the fc portion of bovine immunoglobulin g1 in whey but not in serum or as a purified protein from serum. supernates from strains 19 and 2308 of b. abortus were both capable of this hydrolysis whereas living cells were not. the cleavage process was independent of antibody activity to b. abortus, appeared to require factor(s) found only in some whey samples and was ineffective with the other bovine immunoglob ...19853930059
delayed development of humoral immunity in chickens following in ovo treatment with mibolerone.in order to determine if mibolerone causes rapid development of the humoral immune system as previously hypothesized, 12-day-old chicken embryos were injected with 0.1, 1, or 10 micrograms mibolerone. mibolerone caused bursal atrophy in a dose-dependent manner with severe atrophy at the 10 micrograms dose, substantial atrophy at the 1 microgram dose, and very little or no atrophy at the 0.1 microgram dose. mibolerone-treated chicks were subsequently challenged with sheep red blood cells (srbc) a ...19853930306
application of a timing protocol to the reduction of inter-plate variability in the indirect enzyme immunoassay for detection of anti-brucella antibody.a simple timing protocol was developed to monitor chromogen conversion in an enzyme immunoassay, performed in microtiter plates, for the detection of antibody to brucella abortus in bovine serum. application of this protocol decreased the inter-plate coefficient of variation from 28.6% to 6.8% when optical density (od) values, subsequent to the reaction of a standard antibody reagent, were compared to a static development time. substantial reductions in variation were also observed for low titer ...19853930575
serological investigation on cattle vaccinated with a killed brucella abortus strain 45/20 adjuvant vaccine.serum antibody reactions were monitored for a period of 18 months in 18 heifers which had been vaccinated with brucella abortus killed 45/20 adjuvant vaccine, using four standard serological procedures: the rose bengal, serum agglutination, complement fixation and coombs antibovine globulin tests. the rose bengal and serum agglutination tests were persistently negative during the experiment. titres to the complement fixation test rose sharply for only four weeks after the second dose of the vacc ...19853932247
evaluation of brucellin skin test for bovine brucellosis.the diagnostic efficiency of the brucellin skin test for bovine brucellosis was evaluated using cattle of known history. the test was negative in six out of 14 heifers (42.9%) infected with virulent brucella abortus (br. abortus) strain 544. in four cattle vaccinated with a reduced dose of br. abortus strain 19 vaccine (5 x 10(9) live organisms) the skin test became positive in all the animals but two weeks after immunization. however, all the vaccinates became negative 14 weeks after vaccinatio ...19853932248
efficacy of long-acting oxytetracycline alone or combined with streptomycin in the treatment of bovine brucellosis.twenty-nine brucella abortus culture-positive cows were treated with a long-acting oxytetracycline (20 mg/kg of body weight, im) alone or combined with streptomycin (25 mg/kg, im or iv) or were re-treated with the same product. there appeared to be a synergism by the 2 drugs. of 21 courses of treatment with the combined antibiotics, 14 (67%) were considered successful. only 3 of 14 (21%) were successful using oxytetracycline alone. the period from onset of therapy to cessation of shedding in udd ...19853932272
an evaluation of a biphasic medium for the isolation of brucella abortus from bovine tissues.the isolation of virulent brucella abortus from specimens taken from cattle was studied using culture in biphasic medium in which solid and liquid media were contained in the same flask and on solid medium in petri plates. a total of 8638 specimens from 441 cattle, reactors to one or more of several serological tests or to an allergic test, were used. the specimens consisted of lymph nodes, udder, spleen, uterine caruncles, cotyledons, foetal tissues, foetal stomach contents and mammary secretio ...19853933471
an enzyme-linked immunosorbent assay for detection of brucella abortus in cattle using monoclonal antibodies.one group of 24 cattle was vaccinated with the usual calfhood dose of b. abortus strain 19 and a further 27 cattle were similarly vaccinated but as adults. twenty-four cattle (12 from each group) and a control group of 12 cattle were subsequently challenged with b. abortus strain 544. two monoclonal antibodies (ma (a) and ma (b) ) conjugated to horseradish peroxidase were used independently in a competitive enzyme-linked immunosorbent assay (elisa) to test the serums. after vaccination with b. a ...19853933472
the effects of in ovo mibolerone treatment on the bursa of fabricius and the humoral system of chickens: a dose-response study.the effects of various doses of mibolerone, an analog of testosterone, in the development of the bursa of fabricius and the humoral immune system were examined by injecting 0.01, 0.1, 1, 10 or 100 micrograms mibolerone into the chorio-allantoic cavity of 12-day-old chick embryos. treatment with 0.01 or 0.1 micrograms of mibolerone caused slight or no bursal atrophy, 1 microgram caused substantial bursal atrophy, and 10 or 100 micrograms caused severe bursal atrophy at the time of hatching and at ...19853934110
the instability of brucella abortus strain 45/20 and a note on significance of using an unstable rough strain in the diagnosis of bovine brucellosis.the serum agglutinin titres of six heifers experimentally inoculated with live br. abortus strain 45/20 were similar to those produced in six bullocks inoculated with virulent br. abortus strain 544. however, the agglutinin response appeared slowly and disappeared earlier in the heifers than in the bullocks. it is concluded that br. abortus strain 45/20 is an unstable strain, could revert to the smooth form and become highly agglutinogenic in non-pregnant heifers. the significance of using an un ...19853935591
differentiation of brucella abortus and yersinia enterocolitica serotype 09 infections: use of lymphocyte transformation test.cell-mediated immune responses in cattle infected with brucella abortus strain 544, cattle infected with yersinia enterocolitica serotype 09 and non exposed cattle were studied by an in vitro whole-blood lymphocyte stimulation procedure. a soluble brucella polypeptide containing some lipopolysaccharide prepared from brucella abortus strain 99 was used as antigen while concanavalin a was used as mitogen. results were assayed for (6-3h) thymidine incorporation into deoxyribonucleic acid. all the c ...19853935592
transient enhancement of the serum antibody response to brucella abortus strain 19 in cattle treated with levamisole.two experiments were done on 57 steers. these cattle were allotted to 8 groups (4 groups/experiment) and vaccinated with 1 to 3 x 10(9) colony-forming units of brucella abortus strain 19. cattle in 3 of the 4 groups/experiment were given 6 mg of levamisole/kg, subcutaneously, either at the time of vaccination (day 0), 7 days later, or at both times. serum antibody titers to b abortus were measured sequentially for 28 days in experiment 1 and for 56 days in experiment 2, using the card test, riva ...19853936383
the lymph-borne response of foetal lamb lymph nodes to challenge with brucella abortus in utero.the cannulated prescapular lymph node of the foetal lamb was challenged with killed brucella abortus. usually, both efferent prescapular ducts were cannulated and one node left as a control. nodes were given primary or secondary challenges with doses of 10(9) - 2 x 10(10) brucella organisms and the lymph-borne response of the nodes followed. the foetal lymph node produced a vigorous cellular response to the injected brucella, restricted to the challenged side. the total cell output and, more str ...19853936464
antibody response in partridge (perdix perdix l.) 1. effect of sex and age on the immune response to sheep red blood cells (srbc), newcastle disease virus (ndv) and brucella abortus (buck 19).to ascertain the immune response capacity of partridges to classical experimental and natural antigens, dose and time response curves to sheep red blood cells (srbc), newcastle disease virus (ndv) and brucella abortus (buck 19 strain), were determined in two strains reared in two different farms. 1 ml of 20% srbc, 10(9) ndv particles and 10(7) buck 19 microorganisms, gave optimal antibody responses seven (srbc and buck 19) or fourteen days after immunization. as far as srbc is concerned, the ant ...19853936735
cell titration assay for measuring blastogenesis of bovine lymphocytes.the blastogenic response of bovine peripheral blood lymphocytes to phytohemagglutinin (pha) and to microbial antigens was measured using a lymphocyte titration assay. culture conditions, including lymphocyte concentrations, incubation periods and medium formulation, were established which produced linear or nearly linear responses over a range of cell concentrations. these conditions were established by testing lymphocytes from unimmunized cattle and from heifers infected with brucella abortus w ...19853937321
immune response of cattle to brucella abortus outer membrane proteins measured by lymphocyte blastogenesis.lymphocytes from cattle were tested in a blastogenesis test with outer membrane proteins isolated from smooth strain 2308 and rough strain 45/20 of brucella abortus. the titration assay developed for measuring blastogenesis to microbial antigens (baldwin, antczak and winter, this issue, pp. 319-333) was used to assess the response to both group 2 (porins) (douglas et al., 1984) and group 3 proteins (verstreate et al., 1982). blastogenesis was evaluated for distinguishing cattle infected with vir ...19853937324
[incorporation of the protective antigen of brucella abortus into liposomes and the immunogenic properties of the antigen-containing liposomes].the incorporation of b. abortus protective antigen into liposomes and its localization in liposomes have been found to depend on the lipid composition of liposomes. after the injection of the protective antigen conjugated with negatively charged liposomes humoral response is more pronounced than after the injection of the protective antigen incorporated into neutral liposomes. the immunization of guinea pigs with antigen-containing liposomes ensures the production of "incomplete antibodies" in t ...19853937400
structure and serology of the brucella abortus o-antigen. 19853937754
effects of challenge dose on the clinical and immune responses of cattle vaccinated with reduced doses of brucella abortus strain 19.fifty-seven pregnant beef heifers that were unvaccinated or previously vaccinated with brucella abortus s19, at a dose of either 10(9) or 10(10) colony-forming units (cfu), were challenge-exposed intraconjunctivally with virulent b. abortus s2308 at a dose of 9.4 x 10(6) cfu (experiment 1) or 5.2 x 10(7) cfu (experiment 2). in experiment 1, s19 afforded significant protection (p less than 0.01) against challenge exposure in that 8 of 9 unvaccinated heifers, 1 of 11 vaccinated with 10(9) cfu, and ...19853938101
serological response of cattle following brucella abortus strain 19 vaccination and simultaneous administration of levamisole.the serological response of heifers to injection with brucella abortus strain 19 vaccine and simultaneous administration of levamisole hydrochloride were compared with those of heifers inoculated with brucella abortus strain 19 vaccine alone. the rose bengal, serum agglutination, complement fixation and coombs' antibovine globulin tests were used to measure the humoral responses for a period of sixteen weeks after vaccination. the results indicated that antibody titres in the heifers injected wi ...19853938779
[production of brucella abortus strain 19]. 19853939262
[detection in bovine serum of non-agglutinating antibodies directed against occult antigens of brucella abortus strain 19].injection of different animal species with particulate t-dependent antigens induces the appearance of two antibody populations belonging to igg class, one of them with agglutinating activity and the other lacking this capacity, that is, non-agglutinating. in this paper, 3 adult female hereford were inoculated subcutaneously, fortnightly, four times, with 5 x 10(10) heat killed smooth s19 brucella abortus cells. blood samples were taken ten days after the last inoculation and were individually pr ...19853939693
a case of brucellosis complicated by endocarditis and disseminated intravascular coagulation.brucellosis, being eradicated among domestic animals in some countries, is still prevalent in some others where it poses a potential threat to the consumers of milk and cheese and those working with animals and meat. the patient presented below had contracted a severe and long-standing brucella abortus infection by ingesting raw camel milk. she had signs of endocarditis and disseminated intravascular coagulation, but recovered when treated with tetracyclin and streptomycin.19853976429
immunomodulation of the antibody response to lipopolysaccharide in c3h/hej mice by complexing with heterologous ribosomes.we show that formaldehyde fixation of salmonella typhimurium lipopolysaccharide (lps) to ribosomes purified from brucella abortus induced a primary immunoglobulin m (igm) response to lps in c3h/hej mice and upon revaccination resulted in elevated titers of igm and induction of igg antibody to the o antigens of lps, as measured by an enzyme-linked immunosorbent assay. a similar lps-aspergillus fumigatus ribosomal complex yielded igm and igg antibody to lps only after secondary stimulation. these ...19853980087
non-specific reactions to the brucella abortus sat. 19853984182
non-specific reactions to the brucella abortus sat. 19853992840
immune responsiveness and lymphoreticular morphology in cattle fed hypo- and hyperalimentative diets.immune responses and lymphoreticular morphology were studied in 3 groups of yearling hereford steers fed hypoalimentative, maintenance and hyperalimentative diets for 142 days. significant decreases in plasma protein levels and circulating lymphocyte levels occurred in low energy intake steers. percent circulating lymphocytes bearing surface immunoglobulins and serum levels of igg and igm did not vary significantly within or between groups. antibody responses to brucella abortus bacterin inocula ...19853992909
the effect of reduced-dose brucella abortus strain 19 vaccination in accredited dairy herds. 19854063777
reversion of rough (r) to smooth (s) forms in brucella abortus. i. the manner of manifestation of r-s reversion. 19704099090
blocking serum lysis of brucella abortus by hyperimmune rabbit immunoglubulin a. 19714103920
the relationship of brucella abortus agglutinogenic antigens to the receptor sites for tbilisi phage. 19724111734
the direct fluorescent antibody test for detection of brucella abortus in bovine abortion material.the direct fluorescent antibody test was assessed for detection of brucella abortus in bovine abortion material. br. abortus organisms could be readily detected as characteristic intra-cellular clumps, in smears stained with fluorescent antibody. counterstaining with evans blue was necessary to suppress tissue auto-fluorescence. the method was specific and enabled brucella infection to be differentiated, inter alia, from q-fever infection.19734121013
serological cross-reactions between different brucella species and yersinia enterocolitica. immunochemical studies on phenol-water extracted lipopolysaccharides from brucella abortus and yersinia enterocolitica type ix. 19734125692
serological cross-reactions between different brucella species and yersinia enterocolitica. agglutinating activity of 19 s and 7 s antibodies aginst somatic antigen of brucella abortus and yersinia enterocolitica type ix. 19734128117
serological cross-reactions between brucella abortus and yersinia enterocolitica ix. 19744141713
incomplete restoration of the bursa-dependent immune system after transplantation of allogeneic stem cells into immunodeficient chicks.transplantation of allogeneic cells from bursa of fabricius into cyclophosphamide-treated, immunodeficient chicks resulted in immunological tolerance to donor line skin grafts; graft-versus-host disease did not occur. allogeneic bursal stem cells taken from 3-day-old donors induced restoration of bursal morphology, of antibody formation to brucella abortus and of occurrence of pyroninophilic cells and immunoglobulin-bearing cells in the peripheral lymphoid tissues. secondary response to sheep re ...19744150769
[typing of 800 strains of brucella abortus isolated from aborted cattle embryos]. 19644157875
electrophoretic studies of sera from cattle vaccinated or naturally infected with brucella abortus.examinations of the electrophoretic behaviour in cellulose acetate were made on the proteins of sera from 18 field cases of brucellosis, 15 calves recently vaccinated with strain 19 brucella abortus and from 28 normal cattle. the total protein and gamma-globulin content of the sera of the naturally infected, serologically positive cattle tended to be somewhat higher than that of sera from most normal cattle. a few of the serologically-negative normals also had defenitely elevated serum gamma-glo ...19664164271
[contribution on the differentiation within the species brucella abortus by means of antibiotics]. 19664171010
lymphoepithelial tissues of the intestine and differentiation of antibody production.the antibody response of young rabbits to the injection of killed brucella abortus was abolished by prior extirpation of intestinal lymphoepithelial tissues combined with lethal xirradiation and reconstitution with rabbit fetal liver cells. extirpative surgery alone, like lethal x-irradiation and reconstitution without surgery, did not abolish this response. intestinal lymphoepithelial tissues of rabbits apparently play a central lymphoid function and are responsible for the differentiation of t ...19684173040
antibody and immunoglobulin production at the cellular level in bursectomized-irradiated chickens.the effect of bursectomy combined with sublethal x-irradiation in the newly hatched chicken on the immunoglobulin and antibody producing capacity in later life was investigated. the previous findings of a significant incidence of hypogammaglobulinemia in such animals were confirmed. spleen cells from severely hypogammaglobulinemic animals synthesized and secreted little or no immunoglobulin. such spleen lymphoid cells contained fewer immunoglobulin antigenic determinants than spleen cells from i ...19694181832
[ratio of serum antibodies in the immune response to brucella abortus]. 19704191371
[increase in the immunostimulation properties of inactivated brucella abortus b 19 by simultaneous use of a specific immune serum]. 19734197497
[lysogenic variants of brucella abortus. 3. a study of the allergic reaction in experimentally infected laboratory animals]. 19724197603
a serological investigation on adult cattle vaccinated with brucella abortus strain 19. 19734197922
abortion associated with brucella abortus (biotype 1) in the t.b. mare. 19734198441
serological cross-reactions between different brucella species and yersinia enterocolitica. biological and chemical investigations of lipopolysaccharides from brucella abortus and yersinia enterocolitica type ix. 19734199585
the survival of brucella abortus in milk and milk products. 19734200287
stimulation of anti-brucella vaccination in mice by tetramisole, a phenyl-imidothiazole salt.the effect of tetramisole (hydrochloride of racemic 2, 3, 5, 6,-tetrahydro-6-phenyl-imidazo [2, 1-b] thiazole) on immunization was investigated in mice vaccinated by killed brucella melitensis cells suspended in incomplete adjuvant. immunity to brucella abortus challenge was estimated by the reduction in number of b. abortus colonies per gram of spleen in those mice which escaped full immunization and also by calculation of mean infective doses for each group of mice. all tetramisole treatments ...19734200541
immune response to a syngeneic rat tumour: evolution of serum cytotoxicity and blockade. 19734202457
the immuno-diffusion method for the identification of cattle vaccinated with brucella abortus strain 45-20. 19734202541
the sensitivity of brucella abortus to chemotherapeutic agents. 19734202898
assessment of fluorescent antibody absorption procedures for differentiation of the serological response to yersinia enterocolitica serotype ix and brucella abortus in cattle. 19734203694
the incidence of the various biotypes of brucella abortus in cattle in the south-west of scotland. 19734204008
[on the bactericidal power of samples of human blood against strains of brucella melitensis and brucella abortus]. 19724205694
glucose transport in brucella abortus.brucella abortus british strain 19 transported glucose with an apparent k(m) of 0.16 mm and an apparent v(max) of 250 nmol per min per mg of n. the only common glucose analogue transported was 2-deoxyglucose (2-dog), with an apparent k(i) of 0.73 mm. alpha- or beta-methyl glucosides and 3-o-methylglucose were not transported. transport was linear for 70 to 90 s, depending on the concentration of substrate used. 2-deoxyglucose was transported as the free sugar and was not further metabolized once ...19744206873
occurrence of quinovosamine in lipopolysaccharides of brucella species.lipopolysaccharides obtained from brucella abortus, b. melitensis, and b. suis, but not b. canis, were found to contain amino sugars identified as glucosamine and quinovosamine by cation exchange and thin-layer cellulose chromatography and ninhydrin degradation.19744207190
isolation of substances responsible for lymphokine activity from sensitized mouse spleen cells.cultures of brucella-sensitized mouse spleen cells exposed to brucella abortus antigens in vitro release macrophage migration inhibition factor (mif) and macrophage spreading factor. subjecting the supernatants from such cultures to preparative scale electrophoresis in acrylamide gel yields several fractions, one of which contains both mif and macrophage spreading factor. this material has properties attributable to guinea pig mif: it is nondialyzable, heat stable, nontoxic to macrophages from h ...19744208528
[greater attenuation of the virulence of brucella abortus 19 with preservation of its immunogenicity]. 19734208623
effect of brucella abortus infection (vaccine strain 19ba) on rauscher leukemia virus and l1210 leukemia in mice. 19744208714
studies on a smooth phage-resistant variant of brucella abortus. i. immunological properties. 19744209104
interferon induction in mice by lipopolysaccharide from brucella abortus.endotoxin or lipopolysaccharide isolated from brucella abortus (strain 456) produced a typical endotoxin-type interferon response in mice with peak levels of the inhibitor occurring 2 h after intravenous injection of the stimuli. brucella lipopolysaccharide was a much less effective interferon stimulus than the lipopolysaccharide isolated from salmonella typhimurium (strain lt-2).19744210341
effect of aflatoxin on serum proteins, complement activity, and the antibody response to brucella abortus in guinea pigs. 19744211951
[virulence of brucella abortus species l forms for guinea pigs]. 19744212877
the rose bengal plate agglutination test in dairy cattle in zambia vaccinated over age with strain 19 brucella abortus. 19734213481
[antigenic competition. response to a second antigen (sheep erythrocytes) in an animal persistently immunized with brucella abortus]. 19744213980
the incidence of brucella infections in producer-retailer herds in north lancashire from 1965 to 1972.the results are presented of testing untreated producer-retailer herd milk samples for the presence of brucella abortus during the period 1965-1972 in the north lancashire region.there was a steady decline in the incidence of infected herds from 22% in 1965 to 12% in 1971. a sharp fall to 5% in 1972 suggests that the introduction of the brucellosis incentives scheme and the eradication programme has helped to reduce the practice of selling brucella-infected cattle in the open market which was pr ...19744213998
brucella abortus biotype 3 in tanzania. 19744215211
genetic (h-2) dependence of the duration of splenomegaly induced by brucella abortus. 19744215683
milk hygiene and bacteriology in the sudan: isolation of brucella abortus from cow's milk. 19744220143
[rapid biochemical methods for the differentiation of brucella suis and brucella abortus]. 19664224555
chromatographic studies of sera from calves vaccinated with brucella abortus, strain 19.the presence of antibody was detected by agglutination tests in the serum of calves four days after vaccination with brucella abortus strain 19. titres had reached a maximum by seven to ten days post-vaccination. sucrose density-gradient ultracentrifugation demonstrated that the earliest antibodies were macroglobulins, igm (19sgamma; gammam)-globulins. lighter antibodies, igg (7sgamma(2); gammag)-globulins, appeared a few days later. with time, antibody titres fell, igm declining somewhat more q ...19674226677
a preliminary study of the development of 19s and 7s immunoglobulins in sera of two sheep vaccinated with brucella abortus, strain 19.the pattern of antibody response to vaccination with brucella abortus, strain 19, was studied in two sheep. agglutinative activity was detected by the third and fifth days and complement - fixing activity by the fifth and seventh days post-vaccination. density gradient centrifugation and deae cellulose column chromatography showed the 19s antibody developed first, followed soon after by 7s antibody. the former had disappeared by the 25th day but the latter persisted longer in both sheep. a small ...19694237299
evaluation of various media and the use of soil infusion in the production of brucella abortus antigen.brucella abortus (adri 413) was grown on various synthetic, commercial, laboratory-formulated and soil infusion-supplemented agar media. of 22 media tested, three soil infusion-supplemented media and a serum dextrose agar yielded more brucella antigen than the meat infusion agar, the standard reference medium. results from agglutination and stability tests indicated that antigens produced from most of the laboratory-formulated media and the soil infusion-supplemented agars compared favourably wi ...19734265551
the utilization of brucella abortus culturing and biotyping results in the epizootiologic investigation of bovine brucellosis. 19734283853
antigenic relationship of the gram-negative organism causing canine abortion to smooth and rough brucellae.the use of whole-cell antigens in agglutination and agglutinin-absorption tests showed that the organism causing abortion in dogs is similar to rough brucella abortus, b. melitensis, and b. ovis, but different from smooth brucella cultures. water-soluble antigens obtained by ultrasonic treatment and examined by immuno-electrophoresis and gel diffusion show extensive cross-reactions within the genus brucella, but little or no cross-reaction with similar antigens from other gram-negative genera in ...19684295848
brucella abortus in fresh cream and cream products. 19684297595
[brucellosis caused by brucella abortus in humans in france. a survey at indre-et-loire]. 19704316963
the hygiene and marketing of fresh cream as assessed by the methylene blue test. a report by a working party to the director of the public health laboratory service.the hygiene and marketing of fresh cream in england and wales was investigated by a working party of the public health laboratory service (phls) between 1 october 1968 and 31 july 1969. thirty-one cream-producing dairies were visited and observations made in the light of the code of practice published by the milk and milk products technical advisory committee of the ministry of agriculture, fisheries and food, and the scottish home and health department. suggestions are made in this report to st ...19714326247
[neutral lipids of brucella abortus]. 19714329697
mechanism of resistance to virus challenge in mice infected with brucella abortus. 19724343370
specific secondary biological properties of purified rabbit immunoglobulin m and immunoglobulin g antibodies to brucella abortus and bordetella pertussis.rabbit immunoglobulin (ig)m and igg antibodies to brucella abortus and bordetella pertussis were isolated as purified products and their specific secondary biological activities were compared. igm antibodies were found to be more active than igg proteins in inducing agglutination and sensitization of b. abortus for the complement-dependent bactericidal effect and in inhibiting b. pertussis-induced lymphocytosis in the mouse. igm and igg antibodies were found to be equally effective in inducing a ...19714343404
protection of rabbits against local vaccinia virus infection by brucella abortus and polyacrylic acid in the absence of systemic interferon production.brucella abortus and polyacrylic acid, two agents whose interferon-inducing capacity in the mouse has been well established, failed to stimulate circulating interferon production in rabbits, yet protected rabbits against local vaccinia virus infection.19734355140
the development of a new selective medium for the isolation of brucella abortus from contaminated sources. 19744369280
antiviral activity of an ether-extracted nonviable preparation of brucella abortus.extraction of living brucella abortus (strain 456) with aqueous ether yielded a nonviable, insoluble residue (bru-pel). when injected into mice, bru-pel was an effective, nontoxic interferon stimulus. mice pretreated with bru-pel were protected against challenge with otherwise lethal doses of semliki forest virus. significant protection was afforded when bru-pel was given as many as 7 days before virus challenge. evidence is presented dealing with the complex nature of bru-pel and with the possi ...19744373393
[duration of the immunity provided by the h 38 killed vaccine against bovine brucella abortus infections]. 19674378356
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