helicobacter-associated duodenitis and gastric metaplasia in duodenal ulcer patients.biopsy specimens were taken from the duodenal bulb and the distal duodenum in 45 duodenal ulcer patients before and after treatment with histamine-2 antagonists, prostaglandin analogues or antacids. after four weeks of treatment, the ulcer had healed in 31 patients. the treatment did not lead to a reduced frequency of helicobacter-associated duodenitis or gastric metaplasia of the duodenal epithelium. we found gastric metaplasia in 52.3% of all biopsy specimens from the duodenal bulb, chronic ac ...19911683540
[diagnosis of campylobacter pylori in chronic gastritis, gastric ulcer and duodenal ulcer (review of the literature)]. 19901695273
iv workshop on gastroduodenal pathology and helicobacter pylori. bologna, november 28-30, 1991. abstracts. 19911684305
acridine orange fluorescence, campylobacter pylori, and chronic gastritis.endoscopic gastric biopsy specimens from 230 consecutive patients in a north liverpool district were histologically studied by routine light microscopy and ultraviolet fluorescence after acridine orange staining. eighty patients with chronic gastritis were further studied with regard to type of gastritis and its activity, presence of campylobacter pylori (cp), and degree of colonization of the gastric mucosa. miscellaneous gastritis, gastric ulcers, erosions, neoplasms, and histologically normal ...19901689506
comparison of culture and histology for the identification of helicobacter pylori in endoscopic biopsies.the aim of the study was to compare culture with microscopic examination of haematoxylin and eosin stained tissue sections in the identification of helicobacter pylori in gastric antral mucosa. duplicate gastric antral biopsies from 89 patients were cultured and examined histologically for h pylori. h pylori was cultured in 46 cases (51%) and identified histologically in 63 (71%). results from the two tests concurred in 63 cases (71%). the presence of h pylori was strongly correlated with histol ...19901697451
use of dna restriction endonuclease digest and ribosomal rna gene probe patterns to fingerprint helicobacter pylori and helicobacter mustelae isolated from human and animal hosts.variation amongst strains of helicobacter pylori and helicobacter mustelae was examined by dna restriction endonuclease digestion and rrna gene patterns generated using a non-radioactive probe. chromosomal dna was extracted from 30 cultures of h. pylori from human, rhesus monkey and pig gastric mucosa, and from three h. mustelae isolates from ferret gastric mucosa. dna fingerprinting with hae iii and hind iii showed h. mustelae was relatively homogeneous but revealed genomic heterogeneity within ...19901698260
detection of antibodies to helicobacter pylori cell surface antigens.serum igg antibodies of helicobacter pylori were detected in single-dilution elisa using glycine extracted material. among 148 endoscopy patients 59% displayed antibodies; as expected, a higher occurrence (90%) was found in patients with positive gastric culture for h. pylori than in culture negative patients (37%). among 68 blood donors the frequency of h. pylori antibodies was 28%. in 73 children less than 15 years of age examined for unrelated disorders the occurrence was 4%. by immunoblottin ...19901699265
a spiral microorganism in the stomach of pigs.light and electron microscopic and microbiologic evaluations were performed on mucosa of stomachs from 120 healthy slaughtered pigs. helicobacter pylori was not found, but a tightly spiralled bacterium, not previously described, was seen in histological sections and/or in carbol fuchsin stained smears in 13 (10.8%) stomachs. in paraffin sections stained with carbol fuchsin, the bacteria were seen in the mucus of the lumen of the antral pits and in the mucosa surface within and beneath the mucus. ...19901700535
[a method of isolating campylobacter pylori from the gastrointestinal mucosa]. 19901692368
[diagnosis of campylobacter pylori based on the level of urea in the gastric juice].comparison of urea levels in 3 portions of gastric juice, obtained on an empty stomach, basal, and stimulated, from 28 patients, c. pylori carriers, and 20 patients without c. pylori carriership, has shown that the presence of this bacterium in the gastric mucosa is related not so much with a decrease in the absolute level of urea in the portion collected on an empty stomach but mostly with changes in this parameter in three portions of gastric juice collected in succession. decreased urea level ...19901695282
comparison of four stains and a urease test for rapid detection of helicobacter pylori in gastric biopsies.gastric biopsies were obtained from 125 subjects to compare detection of helicobacter pylori by culture, a rapid urease test and histopathologic examination using haematoxylin-eosin, gram, giemsa, warthin-starry silver and acridine orange stains. helicobacter pylori was isolated from 39 specimens. acridine orange and giemsa were the most sensitive stains, detecting 85% and 79% of positive specimens respectively. all stains showed high specificity (97-100%). the sensitivity and specificity of the ...19901695573
direct gram stain and urease test to detect helicobacter pylori.antral biopsies were obtained by gastrointestinal endoscopy on 143 adult patients with dyspeptic symptoms of gastritis or peptic ulcer disease. a direct gram stain and a direct urease test were performed on each biopsy in addition to culture. forty-three biopsies (30%) were considered positive for helicobacter pylori based on culture or histologic examination, or both. thirty-one biopsies (72% sensitivity) were positive for both direct tests, whereas 95 of 100 negative cultures were negative for ...19901703938
direct detection and amplification of helicobacter pylori ribosomal 16s gene segments from gastric endoscopic biopsies.helicobacter pylori is an organism thought to play an important causative role in gastritis and peptic ulcer diseases. we have designed an rna dot blot assay for the detection of h. pylori, using as probe a synthetic oligonucleotide complementary to its 16s rrna. we have also used oligonucleotide primers, complementary to conserved sequences within bacterial ribosomal 16s genes, to amplify a h. pylori ribosomal 16s dna fragment via the polymerase chain reaction (pcr). after determining the dna s ...19901703940
identification, characterization, and spatial localization of two flagellin species in helicobacter pylori flagella.flagellar filaments were isolated from helicobacter pylori by shearing, and flagellar proteins were further purified by a variety of techniques, including cscl density gradient ultracentrifugation, ph 2.0 acid disassociation-neutral ph reassociation, and differential ultracentrifugation followed by molecular sieving with a sephacryl s-500 column or mono q anion-exchange column, and purified to homogeneity by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transfer to an ...19911704004
fine structure of active and healed duodenal order to characterize the fine structure of active and healed duodenal ulcers, we examined tissue specimens of patients with active duodenal ulcer disease (n = 30) before and after treatment with either antacids (n = 16) or h2-receptor antagonists (n = 14), by light microscopy and various electron microscopic techniques, e.g., scanning and transmission electron microscopy. the characteristic histological feature of both the active and healed duodenal ulcer was the appearance of periodic acid- ...19911704181
phylogeny of helicobacter felis sp. nov., helicobacter mustelae, and related bacteria.strain cs1t (t = type strain) is a gram-negative, microaerophilic, urease-positive, spiral-shaped bacterium that was isolated from the gastric mucosa of a cat. additional strains which possessed biochemical and ultrastructural characteristics similar to those of strain cs1t were isolated from the gastric mucosa of cats and dogs. the guanine-plus-cytosine content of the dna of strain cs1t was 42.5 mol%. the 16s rrna sequences of strain cs1t, strain ds3 (a spiral-shaped isolate from a dog), and he ...19911704791
cytodiagnosis of campylobacter pylori in papanicolaou-stained imprints of gastric biopsy specimens.the use of papanicolaou-stained touch preparations of gastric antral biopsies for the identification of campylobacter pylori was examined using specimens obtained from 63 consecutive patients with endoscopic evidence of antral gastritis, with the results compared to routine histologic examination and warthin-starry silver staining. organisms were readily identifiable in the papanicolaou-stained imprints of the gastric mucus. the sensitivity in detecting organisms was 92.5% for the warthin-starry ...19911709322
characterisation of the urease from helicobacter pylori and comparison with the ureases from related spiral gastric bacteria.the urease enzyme of helicobacter pylori was partially purified from whole cell extracts and found to have a molecular weight of 484 +/- 12 kda. ten monoclonal antibodies (mabs) were produced against four different epitopes of the native enzyme. these mabs also recognised the ureases of h. pylori-like organisms isolated from monkeys and pigs and the h. mustelae urease from ferrets. the urease enzymes of each of these organisms were found to be of the same molecular weight. the urease enzyme of h ...19911711875
comparison of sensitivity and specificity of three different methods for the detection of helicobacter pylori infection. 19901701286
[helicobacter pylori--a pathogen with many faces]. 19911714173
[virulence factors of helicobacter pylori and pathomechanisms of mucosal damage]. 19911714174
[effect of helicobacter pylori on stomach physiology]. 19911714175
[helicobacter pylori infection: a facilitator of stomach cancer?]. 19911714176
[epidemiology and diagnosis of helicobacter pylori infection]. 19911714177
[helicobacter pylori infection, gastritis and non-ulcer dyspepsia--is there a connection?]. 19911714178
[methods of isolating and identifying campylobacter pylori (review of the literature)]. 19911714997
rapid diagnosis of campylobacter pylori infection by urea test paper.antral biopsy specimens from 106 patients were examined by culture, gram stain and silver stain for campylobacter pylori. biopsies were also examined by a urea test paper test (utpt). of 106 patients studied c. pylori was detected in 68 (64.2%) by gram stain, silver stain and culture. the utpt was positive in 63 (59.4%) specimens. five had false negative results using the utpt with no false positive subjects. thus, utpt has a sensitivity of 92.6% (63/68) and a specificity of 100%. of the 63 spec ...19901717032
detection of helicobacter pylori in stomach tissue by use of a monoclonal antibody.monoclonal antibodies were produced against an acid glycine extract of helicobacter pylori atcc 43504t. one of these appeared to be specific for h. pylori; it recognized all h. pylori isolates by an indirect immunofluorescence assay (iif) but it did not cross-react with the other strains tested, including different species of the genera helicobacter, campylobacter, and wolinella. different strains of members of the families enterobacteriaceae and pseudomonadaceae or other gram-negative bacteria ...19911721923
a high frequency of detection of helicobacter pylori in whitish exudate of gastric ulcer.helicobacter pylori has been implicated in the pathogenesis of chronic gastritis and peptic ulcer. however, no report to date has been made on the presence of h. pylori in the whitish exudate of peptic ulcers. we examined the biopsy specimens of 52 patients (42 men and 10 women, aged 29-78, not taking steroids or nonsteroid antiinflammatory drugs), and evaluated the frequency of detecting the organism in the whitish exudate of gastric ulcers in comparison with that in the ulcer border, gastric f ...19911722231
superior stain for helicobacter pylori using toluidine o. 19901702103
direct polymerase chain reaction test for detection of helicobacter pylori in humans and animals.we designed a polymerase chain reaction (pcr) for amplifying the helicobacter pylori gene encoding 16s rrna. primers for the specific detection of h. pylori were designed for areas of the 16s rrna gene in which there is the least sequence homology between h. pylori and its closest relatives. the specificity of detection was confirmed by ensuring that the primers did not amplify dna extracts from the campylobacters h. cinaedi, h. mustelae, and wolinella succinogenes, which are the closest relativ ...19911723072
[determination of the degree of seeding by helicobacter pylori in the gastric mucosa]. 19911724047
different effect of helicobacter pylori on the human gastric antral and body mucosal intracellular elucidate the role of helicobacter pylori infection in the pathogenesis of gastric ulcer, we investigated the intracellular mucin content by measuring the periodic acid-schiff-alcian blue (pas-ab)-stained substances, by means of computer, in the biopsy sample of gastric mucosa from patients with and without h. pylori infection. in the antral mucosa the intracellular pas-ab-stained mucin content was significantly smaller in patients with infection than in patients without infection, whereas in ...19901702231
[a rapid indication of helicobacter pylori in patients with gastroduodenal pathology].rapid detection of h. pylori in samples from gastroduodenal patients is based on the use of standard ingredients made in the ussr: disks with urea from kits and indicator strips to show ph values of the rifan type, within the ph range 5.8-7.4. this method helps detect ph changes emerging in h. pylori urea disintegration in the liquid and gaseous (air) phases. the sensitivity, specificity, and rate of the test are not inferior to those of the commercial urease test.19911722536
[ultrastructural study of the antral mucosa to determine the presence of helicobacter pylori and its association with chronic active gastritis].we present the results of ultrastructural studies of gastric mucosa obtained through upper digestive endoscopy of eight patients with suspicious symptoms of non ulcer dyspepsia or peptic ulcer. two were male and six female with a median age of 54 years. the urease test to determine the presence of hp and hematoxylin-eosin and warthin-starry staining techniques were practiced with the purpose of a better detection of bacteria and gastritis. we did not find any correlation between the endoscopic r ...19911726915
the usefulness of the acridine-orange stain in identifying helicobacter pylori in gastric biopsies.the efficacy of the acridine-orange stain (aos) in identifying helicobacter pylori (hp)-like organisms in biopsy smears from adults with gastroduodenal disease was studied. the results obtained indicate that aos can replace gram stain in hp organism identification in gastroduodenal mucosa specimen.19911713287
[histologic study of helicobacter pylori in 265 consecutive gastric biopsies].we evaluated 265 consecutive endoscopic gastric biopsies of subjects attending the gastric cancer mass survey that is taken place in the state of tachira, venezuela. they were 152 males and 113 females between 18 and 70 years of age. we established the prevalence of helicobacter pylori in relation with histologic diagnosis and location of the lesions in the stomach. we performed hematoxylin eosin, giemsa and gimenez stains. the bacteria was identified in chronic non atrophic gastritis in 81% of ...19911726912
[helicobacter pylori]. 19911726914
symptoms and risk factors of helicobacter pylori infection in a cohort of identify symptoms and risk factors associated with helicobacter pylori infection, a cohort of 341 epidemiologists was studied. all subjects had one banked serum (collected between 1969 and 1987) and one recent serum sample (collected in 1988) evaluated for h. pylori immunoglobulin g by enzyme-linked immunosorbent assay; subjects provided information on gastrointestinal symptoms and risk factors for gastritis and peptic ulcer disease. prevalence of infection decreased from the early 1970s to t ...19921727779
h. pylori, the most common bacterial infection in africa: a random serological study.this study was carried out to determine the prevalence of antibodies to helicobacter pylori in northern nigeria, a region with a low incidence of peptic ulceration. in a random, serological survey of 268 subjects, 228 (85%) of the population studied had igg antibodies to h. pylori. fifty-eight of these subjects had experienced dyspepsia in the preceding 6 months. the majority of the population (82%) is infected between the ages of 5 and 10. despite the high prevalence of antibodies to h. pylori, ...19921728121
treponema pallidum and helicobacter pylori recovered in a case of chronic active gastritis.a 49-year-old man complaining of epigastric pain underwent endoscopy, during which thickened stomach folds below the fundus were observed. microscopic examination of gastric tissue biopsy specimens revealed chronic active gastritis. dieterle stain revealed overwhelming numbers of "corkscrew-like" spirochetes. these were proved to be consistent with treponema pallidum. a comprehensive study of the tissue revealed the added presence of helicobacter pylori. this appears to be the first case report ...19921728852
cytology: a simple, rapid, sensitive method in the diagnosis of helicobacter pylori.cytology and the rapid urease test on gastric biopsies may diagnose helicobacter pylori (h. pylori) infection within an hour. we evaluated the sensitivity and reproducibility of touch cytology (imprints from biopsies). in 19 patients with duodenal ulcer, biopsies were obtained from the antrum, fundus, and bulb. h. pylori was diagnosed in 42 sites on smears: 28 by culture and 23 by histology. h. pylori was present in the antrum and fundus in 16 and in the bulb in 11. assessment of paired antral b ...19921728119
factors influencing the eradication of helicobacter pylori with triple therapy.helicobacter pylori infection has been associated with gastritis, duodenal ulcer, gastric ulcer, and the epidemic form of gastric carcinoma. eradication of h. pylori infection has proven to be difficult. recently, combinations of antimicrobial drugs have been shown to eradicate greater than 50% of infections; however, the results have proven variable, and the factors influencing effectiveness of therapy are unclear. in the present study, the effectiveness of a triple therapy for eradication of h ...19921732120
hypotheses on the pathogenesis and natural history of helicobacter pylori-induced inflammation.although helicobacter pylori is now recognized as playing an etiologic role in chronic gastritis and peptic ulcer disease, information on the pathogenesis and natural history of infection is limited. a model is proposed in which luminal h. pylori secrete substances that mediate inflammation that is beneficial to the organism but ultimately deleterious for the host; in addition to tissue damage, inflammation also affects gastric secretory function. in this model, the host may attempt to suppress ...19921732141
helicobacter pylori gastric atrophy and pernicious anemia. 19921732153
surface proteins from helicobacter pylori exhibit chemotactic activity for human leukocytes and are present in gastric mucosa.the mechanism by which helicobacter pylori, a noninvasive bacterium, initiates chronic antral gastritis in humans is unknown. we now show that h. pylori releases products with chemotactic activity for monocytes and neutrophils. this chemotactic activity was inhibited by antisera to either h. pylori whole bacteria or h. pylori-derived urease. moreover, surface proteins extracted from h. pylori and purified h. pylori urease (a major component of the surface proteins) exhibited dose-dependent, anti ...19921732414
helicobacter pylori and duodenal ulcer recurrence.preliminary evidence suggests that eradication of helicobacter pylori (h. pylori) may lead to prolonged remission of duodenal ulcer (du). the aim of this study was to assess the long-term effect of eradication of h. pylori on the natural history of du. fifty-one patients with endoscopically proven duodenal ulcers, who were found to have h. pylori infection on histology and culture, and who were successfully eradicated of h. pylori with combination treatment of colloidal bismuth subcitrate and an ...19921728120
helicobacter pylori and gastric lymphonodular hyperplasia in children.the association of gastric lymphonodular hyperplasia and helicobacter pylori infection has been reported only in children. lymphonodular hyperplasia of the stomach is a well known radiographic and endoscopic entity. over the past three decades, it has been associated with many conditions, ranging from a normal variant to a premalignant lesion. we have recently encountered five children with gastric lymphonodular hyperplasia, all of whom had h. pylori infection of the antrum. the literature regar ...19921728111
topographic mapping of helicobacter pylori colonization in long-term-infected pigs.four barrier-born pigs were inoculated with helicobacter pylori during gastroscopy. infection in all pigs was established after 3 weeks, and the animals were kept isolated from other pigs in ordinary experimental sites. the pigs were sacrificed and examined 3, 5, 6, and 6.5 months postinoculation. a detailed urease mapping of the pig stomachs showed a patchy distribution of h. pylori. the bacteria colonized in all pigs, with a concentration of h. pylori-positive areas in the antrum and fundus. f ...19921730499
diagnosis of helicobacter pylori infection in adult and pediatric patients by using pyloriset, a rapid latex agglutination test.pyloriset (orion diagnostica, espoo, finland) is a rapid antibody test using latex particles coated with acid-extracted antigen of helicobacter pylori. we evaluated its ability to predict infection in 100 adult patients and 50 pediatric patients referred for gastric endoscopy. sixty of 65 h. pylori-infected adults were correctly identified by the test. there were 12 false-positive and 5 false-negative reactions seen. pyloriset had a sensitivity of 92% and a specificity of 66%. the positive predi ...19921734072
evaluation of a commercially available second-generation immunoglobulin g enzyme immunoassay for detection of helicobacter pylori infection.we evaluated a commercially available second-generation anti-h. pylori immunoglobulin g enzyme immunoassay (eia) (cobas core anti-helicobacter pylori eia; roche s. a., basel, switzerland) for serodiagnosis of h. pylori infection. the results of the assay were assessed in relation to the results of bacterial culture, urease testing, and histological giemsa stain of gastric biopsy specimens from 1,134 patients with a variety of symptoms relating to the upper gastrointestinal tract. h. pylori was d ...19921734050
15nh4+ excretion test: a new method for detection of helicobacter pylori infection.a noninvasive test for the detection of helicobacter pylori infection that uses [15n]urea as a tracer has been established. the principle the test is based on is the strong urease activity of h. pylori. after oral ingestion, [15n]urea is broken down into ammonia and carbon dioxide by h. pylori urease in the stomach. the ammonia is absorbed into the blood and excreted in the urine. the amount of [15n]urea, reflecting the magnitude of h. pylori infection, is evaluated by measuring the abundance an ...19921734051
sensitive detection of helicobacter pylori by using polymerase chain reaction.a polymerase chain reaction (pcr) for the specific detection of helicobacter pylori was developed with a single primer pair derived from the nucleotide sequence of the urease a gene of h. pylori. we achieved specific amplification of a 411-bp dna fragment in h. pylori. after 35 cycles of amplification, the product could be detected by agarose gel electrophoresis and contained conserved single hinfi and alui restriction sites. this fragment was amplified in all 50 strains of h. pylori tested, but ...19921734052
intestinal metaplasia and helicobacter pylori: an endoscopic bioptic study of the gastric study the relationship between intestinal metaplasia and helicobacter pylori infection, 2274 gastroscopic antral biopsies taken from 533 patients were examined. overall, intestinal metaplasia was found in 135 patients (25.3%) and h pylori in 289 patients (54.2%). the prevalence of intestinal metaplasia and h pylori was age related, being more common in patients greater than or equal to 50 years compared with patients less than 50 years (intestinal metaplasia, p less than 0.001 and h pylori, p ...19921740271
helicobacter pylori and luminal gastric ph. relationships in nonulcer dyspepsia.the relationships between gastric ph and helicobacter pylori infection were studied in 37 consecutive subjects affected with nonulcer dyspepsia. each underwent esophagogastroduodenoscopy with multiple gastric biopsies for both h. pylori and histologic assessment, and 24-hr antral ph monitoring. h. pylori was harbored by 59.5% of the subjects with whole gastric spread of infection in all but one patient. histologic gastritis was shown in 70.3% of the subjects. h. pylori was strongly associated wi ...19921735361
compositional analysis of helicobacter pylori rough-form lipopolysaccharides.sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to analyze the macromolecular heterogeneity of lipopolysaccharides (lps) from seven fresh clinical isolates and three culture collection strains of the human pathogen helicobacter pylori. all the clinical isolates produced smooth-form lps with o side chains of relatively homogeneous chain length, whereas the culture collection strains yielded rough-form lps. a better yield of the latter lps was obtained when combined protease pre ...19921735724
effect of helicobacter pylori on serum pepsinogen i and plasma gastrin in duodenal ulcer patients.duodenal ulcer patients have increased serum pepsinogen i (pgi) concentrations and an increased prevalence of helicobacter pylori infection. we have examined the effect of eradicating the infection on pgi. in 12 duodenal ulcer patients in whom h. pylori was successfully eradicated, the median basal pgi was 90 ng/ml (range, 37-252) before treatment and fell to 74 ng/ml (28-197) 1 month after treatment (p less than 0.01). in 12 patients in whom therapy failed to eradicate the infection, the pgi wa ...19921736337
[helicobacter pylori allergy].a case of a 44 year old woman with antrum gastritis and h. pylori infection was reported. after unsuccessful treatment of the disorder with bismuth and tinidazole, an auto-vaccine was prepared from the bacterium in order to eliminate the infection. after the first injection of the vaccine a generalised urticaria was observed. in the development of the skin eruptions a type i, and a type iv allergic reaction could be demonstrated using the h. pylori specific rast-test and leukocyte migration inhi ...19921741153
association of helicobacter pylori with hla-dr antigen expression in assess the association between helicobacter pylori-associated gastritis and hla-dr antigen (class ii antigen) expression.19921740510
[morpho-functional characteristics of reflux gastritis in patients after cholecystectomy and without cholecystectomy].the aim of this study was to evaluate some biochemical and histopathological aspects in a group of patients with a view to identifying any differences depending on whether the pathology was associated with previous cholecystectomy or idiopathic. the study involved 23 patients (8 post-cholecystectomy cases and 15 ulcer-free dyspeptic patients) with the diagnosis of duodenogastric reflux gastritis confirmed by endoscopic histopathological evaluation. the following parameters were considered: 1) ph ...19911742396
effect of omeprazole on h. pylori after two weeks of treatment.the "clearing" effect of omeprazole 20 mg/daily on helicobacter pylori (hp) was studied in 32 hp positive patients with duodenal ulcer (du). endoscopy and antral biopsies were made before and after two weeks of treatment with omeprazole. hlo test and histology after giemsa stain were used for the detection of hp. fourteen of 28 (50%) initially hlo positive and 12 of 30 (40%) initially histological hp positive patients became hp negative. nine of 23 (39%) patients, initially hp positive by both m ...19911742527
gastric antral erosions and helicobacter pylori infection in cirrhotic patients: a pilot controlled study of oral bismuth vs ranitidine therapy.a controlled study was performed comparing bismuth and ranitidine oral therapy in the treatment of chronic antral erosions and chronic active gastritis and in clearing helicobacter pylori in cirrhotic patients. forty four patients took part in the randomized study. h pylori was present in more than 50% of the patients in the study. there was a close association between the presence of h pylori and inflammatory activity of gastritis at an antral level (p less than 0.001). cbs was significantly be ...19911742507
[development of genetic and molecular approaches for the diagnosis and study of the pathogenicity of helicobacter pylori, agent of gastric inflammatory diseases].helicobacter pylori (h. pylori) is a small gram-negative bacillus, recently discovered, found in the stomach of patients with active chronic gastritis and duodenal ulcers. production of a potent urease has been described as a trait common to all h. pylori so far isolated. to clarify the role of urease in the pathogenic process, as well as to engineer genetic tools useful for the diagnosis of h. pylori, we cloned the genes responsible for urease activity. a genomic library was constructed in esch ...19911742619
in vitro endoscopic polaroid photography for clo test of helicobacter pylori. 19911744411
[helicobacter pylori gastritis: a new infectious disease. reflections from personal experience].to determine the relationship of microscopic chronic antral gastritis to helicobacter pylori, 69 consecutive patients were studied. in a prospective longitudinal study, at least 2 successive gastroscopies with antral biopsies were performed (177 investigations on the whole). sixty six of the 69 patients were treated with various therapeutic regimens i.e. antiulcer drugs and/or antibiotics. the type and intensity of inflammation (as based on whole inflammatory infiltrate density, polymorphonuclea ...19911746875
long-term follow up of patients with gastritis associated with helicobacter pylori infection.the aim of this study was to evaluate the long-term prognosis for patients suffering from gastritis associated with helicobacter pylori infection, and in particular the proportion of cases progressing to peptic ulcer. the study was carried out in one urban general practice. one hundred and three patients who had presented with dyspepsia over the 1973-80 period and who were found to have a macroscopically normal endoscopy were reassessed between seven and 14 years later. gastric antral biopsies h ...19911747267
omeprazole vs. ranitidine in short-term treatment of helicobacter pylori positive duodenal ulcer patients.forty-three patients with active duodenal ulcer and helicobacter pylori positivity in gastric antrum were randomly assigned to either omeprazole treatment (20 mg once a day) or ranitidine treatment (300 mg once a day) for 28 days. re-evaluation of the patients (clinical and endoscopic examination and assessments for h pylori detection) was repeated after 2 weeks and at the end of the treatment. healing rates in the omeprazole group were 40% after 2 weeks and 90% after 4 weeks, in the ranitidine ...19911747501
gastrospirillum hominis associated chronic active gastritis: the first report from italy.recently a spiral bacterium different from helicobacter pylori (hp) was observed in the human stomach and the name of gastrospirillum hominis (gh) was proposed for this organism. gh presence is reported to be not associated to hp but related to chronic active gastritis. we describe the case of a 31 year old male suffering from upper abdominal symptoms, who underwent oesophagogastroduodenoscopy, which revealed a picture of duodenal hyperemia. gastric body showed a normal mucosa and absence of hp, ...19911747510
eradicating helicobacter pylori and symptoms of non-ulcer examine the effect of eradication of helicobacter pylori on symptoms of non-ulcer dyspepsia.19911747644
what does the absence of helicobacter pylori from the antrum imply?helicobacter pylori is absent in the antrum of approximately 5, 25, and 35% of patients with duodenal ulcer, gastric ulcer, and non-ulcer dyspepsia respectively and in the majority of asymptomatic healthy subjects from the west. the absence of h. pylori from the antrum could result from failure of exposure to (group a) or colonization by (group b) or temporary (group c) or permanent (group d) clearance of h. pylori, after initial colonization. marked differences in the incidence of absence of h. ...19911744380
leakiness of gastric superficial and foveolar cells. a quantitative electron microscopic study using tannic acid.two adjacent antral biopsies were taken endoscopically from 41 patients attending a clinic for the investigation of upper gastrointestinal tract symptoms. one was processed for histopathological grading and assessment of the helicobacter pylori by light microscopy. the other was fixed in tannic acid, which demonstrates cell leakiness, and processed for electron microscopy. on each grid, the light (electron lucent) and dark (electron dense-leaky) gastric superficial and foveolar cells away from m ...19911744797
pattern of upper gastrointestinal disease in the eastern province of saudi arabia. endoscopic evaluation of 2,982 patients.the records of 2,982 patients who were subjected to upper gastrointestinal (ugi) endoscopy were retrospectively analyzed. all patients were seen at king fahd hospital of the university, al-khobar during the period 1982-1986. all were adult patients with a mean age of 46 years, of whom two thousand nine (67.4%) were males. the commonest endoscopic diagnosis was chronic gastritis (21.4%) followed by duodenal ulcer (17%) and chronic oesophagitis (11.7%). of 1,256 biopsied patients a 'histologically ...19911750116
immunological response to helicobacter pylori among nigerians.among 420 nigerians examined, 39% had antibodies to helicobacter pylori. the prevalence was significantly higher in males (55%) than in females (29%). the prevalence tended to rise with age in both sexes and was significantly higher in the 30-34 years age group. socio-economic class and rural dwellage did not affect the prevalence. the study may indicate that h. pylori is endemic in nigeria. it also shows that the detection of antibodies to h. pylori may be a rapid and cheap method for diagnosin ...19911750125
helicobacter pylori and the etiology of duodenal ulcer: necessary but not sufficient. 19911750423
is helicobacter pylori a cause of duodenal ulcer? a methodologic critique of current evidence.helicobacter pylori (hp) in the gastric antrum has been strongly associated with both duodenal ulcer (du) and chronic active gastritis (cag). the relationship between hp and du has been interpreted as causal by many observers. an alternate hypothesis is that hp coincidently colonizes cag, which is independently associated with du by some yet-unknown mechanism.19911750424
evaluation of various laboratory techniques to diagnose helicobacter pylori in patients with upper gastro-intestinal tract symptoms.helicobacter (campylobacter) pylori is strongly associated with type b gastritis. the detection of h. pylori, which entails histological examination and culture of gastric biopsy specimens, takes several days. there has been much interest in developing more rapid tests, including non-invasive ones. using histology and/or culture as the 'gold standard', several methods to detect h. pylori were compared and evaluated. the organism was detected in 84 of 100 consecutive patients attending the gastro ...19911745946
effect of partial gastrectomy on serum anti-helicobacter pylori immunoglobulins in peptic ulcer patients.since biliary enterogastric reflux is suggested to eradicate gastric infection with helicobacter pylori (hp), we have investigated in a prospective randomized study the effect of partial gastrectomy with either billroth ii or roux-en-y anastomosis on infection with hp as assessed by the titers of igg and iga antibodies against hp in serum. these antibodies were measured by elisa in serum of 22 patients before and at 10 days and 6, 15, and 24 months after either billroth ii (n = 11) or roux-en-y ...19911748037
differentiation of strains of helicobacter pylori by numerical analysis of 1-d sds-page protein patterns: evidence for post-treatment recrudescence.twenty-three pre- and post-treatment isolates of helicobacter pylori from the antral mucosa of eight patients with dyspepsia and gastritis were compared using 1-d sds page of proteins. the protein patterns were highly reproducible and were used as the basis for two numerical analyses. the first, based on the total protein patterns, showed that a number of the strains did not cluster with their respective patient set. this was thought to be due to differences in both mobility and intensity of pro ...19911752309
a solid-phase enzyme-linked immunospot (elispot) assay for detection of helicobacter pylori antibody-producing cells in gastric mucosa. 19911752401
is helicobacter pylori associated hypergastrinaemia due to the bacterium's urease activity or the antral gastritis?eradication of helicobacter pylori is associated with a fall in serum gastrin but the way in which the infection raises the serum gastrin concentration is not clear. it may be related to the ammonia produced by the bacterium's urease stimulating gastrin release by the antral g cells. alternatively, the antral gastritis induced by the infection may modify the regulation of gastrin release. we have examined serum gastrin in 10 patients before and 24 hours after starting triple anti-h pylori treatm ...19911752456
long term serological surveillance after treatment of helicobacter pylori infection.fifteen patients with type b gastritis caused by helicobacter pylori infection were treated with 'triple' therapy consisting of colloidal bismuth subcitrate, amoxycillin, and metronidazole. all were followed up as outpatients every three months for at least one year. after 'triple' therapy a significant (p less than 0.01) and persistent reduction in iga and igg antibody levels against h pylori was detected. in three patients recurrent active infection with h pylori at nine and 12 months was dete ...19911752457
acute helicobacter pylori infection: clinical features, local and systemic immune response, gastric mucosal histology, and gastric juice ascorbic acid concentrations.the symptomatology of a case of acute infection with helicobacter pylori is described, together with the accompanying changes in gastric mucosal histology, local and systemic humoral immune response, and gastric ascorbic acid concentration. the patient was an endoscopist, previously negative for the carbon-14 urea breath test, who had a week of epigastric pain and then became asymptomatic. h pylori was detected by culture of antral biopsy specimens and was still present after 74 days. five days ...19911752479
helicobacter pylori infection in healthy people. 19911752483
helicobacter pylori infection and non-ulcer dyspepsia: the effect of treatment with colloidal bismuth subcitrate.a study was undertaken to determine the role of helicobacter pylori in non-ulcer dyspepsia (nud) and to determine the efficacy of colloidal bismuth subcitrate (cbs) in the treatment of nud. seventy-one patients were randomly allocated (double blind) to cbs or placebo, two tablets twice daily for 4 weeks. the severity of dyspepsia was scored and endoscopies performed before and after treatment, and antral biopsy specimens were taken for bacteriologic and histologic examination. forty patients had ...19911754846
adhesion of helicobacter pylori to human gastric mucosal biopsy specimens cultivated in vitro.the therapeutic advances in helicobacter pylori infection is hampered by the lack of suitable animal model systems. we have previously reported on successful adherence of h. pylori to pig gastric mucosa cultured in vitro. the aim of this study was to verify the technique in human biopsy specimens cultured in vitro. mucosal samples were taken from h. pylori-negative and h. pylori-positive patients undergoing gastric surgery. the non-infected tissue was infected with h. pylori in vitro, and the in ...19911754854
dental plaque: a permanent reservoir of helicobacter pylori?the aim of the study was to observe the relationship between the two reservoirs of helicobacter pylori--that is, dental plaque and the stomach. with the campylobacter-like organism (clo) test, h. pylori was detected in dental plaque and in gastric antral and body mucosa in 98%, 67% and 70%, respectively, of 43 consecutive patients with dyspepsia. the rapidity of the clo test indicates that the density of h. pylori is heaviest in dental plaque, less in the antrum, and least in the body mucosa of ...19911754858
[bismuth revisited in helicobacter pylori gastro-duodenal infection].the various hypothesis for the explantation of reversible bismuth encephalopathy are reviewed and discussed. today, this side effect appears to be devoid of mystery, due to uncontrolled and inhibited ingestion of bismuth salts which increased the emergence of genetic and physiopathologic risk factors and brought about the cases of neurotoxicity. bismuth therapy has shown major efficacy in the treatment of gastroduodenal ulcers. colloidal bismuth subcitrate is as effective as the h2-receptor anta ...19911754987
non-ulcer dyspepsia in nigeria: a case-control study.non-ulcer dyspepsia is common in africa, yet there are few data on its possible cause. in this study 40 patients with strictly defined non-ulcer dyspepsia were matched with 40 asymptomatic volunteers. both groups were questioned concerning their intake of alcohol, cola nut, non-steroidal anti-inflammatory drugs, and smoking. they then underwent upper gastrointestinal endoscopy with mucosal biopsy of the gastric mucosa: these biopsies were examined for gastritis and helicobacter pylori. ingestion ...19911755074
helicobacter pylori affects the quality of experimental gastric ulcer healing in a new animal model.helicobacter pylori has been implicated in the genesis of human gastritis, dyspepsia, and peptic ulcers. however, its influence in the quality of experimental gastric ulcer healing has not been previously investigated. standardized gastric fundic ulcers were produced in 50 male sprague-dawley rats (150-200 g) by a 4 mm in diameter focal, serosal application of 100% acetic acid. thirty rats were administered 2 ml h. pylori suspension (urease producing, atcc 43504) in normal saline (10(8) cfu/ml) ...19911748215
evaluation of liquid media for growth of helicobacter pylori.helicobacter pylori has routinely been isolated and grown on solid media. recently, we have succeeded in obtaining growth of this organism in several liquid media in large volumes, including tryptic soy broth, mueller-hinton broth, brucella broth, brain heart infusion broth, and columbia broth. growth was tested in the media with and without supplementation. growth was obtained after incubation under microaerobic conditions and with co2 enrichment. growth in a stationary system versus that in an ...19911757556
effect of chewing qat on mucosal histology and prevalence of helicobacter pylori in the oesophagus, stomach and duodenum of yemeni patients.this investigation was aimed at assessing whether the yemeni habit of chewing qat on a regular basis had a significant effect on the upper alimentary tract. seventy patients with dyspepsia attending al-thawra hospital in taiz, yemen republic were examined by endoscopy. biopsies were taken from the oesophagus, stomach and duodenum. the patients included 28 who gave a history of daily qat intake, 21 with less frequent intake and 21 who took none. the only statistically significant finding associat ...19911757083
prevalence of helicobacter pylori in military barracks. 19911751818
in vitro inhibition of helicobacter pylori urease: biochemical and ultrastructural analysis.inhibition of h. pylori urease was studied by means of electron-microscopy and electrophoretic methods using different urease inhibitors, such as acetohydroxamic-acid (aha), l-ascorbic acid (asa), copper ions, a combination of l-ascorbic acid with copper ions and uv light. aha in two different concentrations and asa at a concentration of 0.1 mg ml-1 showed incomplete inhibition of h. pylori urease activity in our electrophoretic experiments. only membrane-bound activity was inhibited with aha bu ...19911752295
young onset peptic ulcer disease and non-ulcer dyspepsia are separate entities.the characteristics of peptic ulcer and non-ulcer dyspepsia in young men were studied in 202 consecutive conscripts who attended central military hospital in helsinki because of long-standing upper abdominal complaints. active peptic ulceration (apu) was found in 48 patients, inactive peptic ulcer disease (ipu) was diagnosed in 77 patients, non-ulcer dyspepsia (nud) was diagnosed in 52 patients. in 25 cases the reason for symptoms was another disease, and these patients were excluded from the st ...19911759126
helicobacter pylori and duodenal ulcer. a study of duodenal ulcer patients and their first-degree relatives.out of 59 duodenal ulcer (du) probands and their 199 first-degree relatives giemsa-staining for the determination of helicobacter pylori (hp) was performed in 51 probands and 155 relatives. controls were matched by age and sex from a family sample representing the same geographical area. in all, 155 controls were found for the probands and relatives. the occurrence and score of hp density showed an excellent correlation with morphology of the mucosa, signs of acute inflammation and presence of g ...19911759128
helicobacter pylori and chronic gastritis: an increased risk of peptic ulcer? a review. 19911759130
grade of helicobacter pylori colonisation, chronic gastritis and relative risks of contracting high gastric ulcers: a seven-year follow-up.a series of 38 patients with high gastric ulcer (gu) was examined twice a seven-year interval. one-third of the patients had an active ulcer at the second examination. chronic gastritis was evaluated, and the level of helicobacter pylori (hp) colonisation assessed semiquantitatively. the results were compared with age and sex matched non-ulcer controls. the gu series differed from the controls in having a higher degree of hp colonisation in gastric mucosa. the relative risks (rr) in predicting h ...19911759132
long-term effect of vagotomy on gastric mucosa and helicobacter pylori in duodenal ulcer patients.the purpose of the study was to evaluate the long-term effects of vagotomy on the morphological status and helicobacter pylori infestation of the gastric mucosa. endoscopy with biopsies (2 from antrum and 2 from corpus) was performed in 317 patients on whom vagotomy had been performed on an average 8 years earlier. the success of the vagotomy was estimated by the endoscopic congo red technique in 270 cases. non-operated 187 duodenal ulcer patients were examined endoscopically and bioptically in ...19911759134
helicobacter pylori: histological and serological study on gastric and duodenal ulcer patients in estonia.we have examined the occurrence of helicobacter pylori (hp) infection in 86 estonian gastric ulcer (gu) and 25 duodenal ulcer (du) patients. diagnosis of the hp infection was made histologically (modified giemsa) from gastric biopsy specimens, and serologically by parallel use of two enzyme-linked immunosorbent assays for igg antibodies to hp in patient sera. the infection was diagnosed simultaneously by all three methods in 84% of the gu and 84% of the du patients. the infection was revealed hi ...19911759135
helicobacter pylori and gastritis in children with abdominal complaints.gastroscopy with biopsies was performed in 178 children with upper abdominal pain. of them 109 (61%) had gastritis and 104 (58%) helicobacter pylori (hp) in antrum and/or corpus. gastritis consisted in most cases (87% of antral and 86% of corpus gastritis) of mild superficial round cell infiltration. mucosal atrophy was not found. accumulation of granulocytes was present in 43 patients and lymphonoduli were found in 33 patients, usually in connection with gastritis. gastritis affected only the a ...19911759137
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