Publications

TitleAbstractYear
Filter
PMID
Filter
cellular events and intracellular survival of campylobacter jejuni during infection of hep-2 cells.invasion and intracellular survival of campylobacter jejuni in hep-2 cells were analyzed by transmission electron microscopy and by viable counts after killing of extracellular bacteria by gentamicin. during the first 30 min after challenge, no bacteria were seen in association with the host cell. after 1 h, campylobacters apparently attached to the cell membrane, with areas of close appositions. in these areas, an intracellular network of actin-like filaments was seen beneath the plasma membran ...19892731988
prospective study of the etiology of diarrhea in adult outpatients and inpatients.a prospective study of the etiology of diarrhea in 253 adult outpatients and inpatients was conducted in helsinki in 1985-86. the outpatients constituted 84.6% of all patients studied. a broad panel of diagnostic techniques was applied, including detection of bacteria and parasites in the feces and determination of the serologic responses to campylobacter, salmonella, yersinia enterocolitica, and several viruses. pathogens were identified for 28.5% of the patients. the commonest findings were ca ...19892734591
[epidemiological surveillance].a case of campylobacter infection in a 2-day old infant is described. the possibility for a newborn infant to get infected with campylobacter jejuni directly from the infected mother in the process of parturition is considered. the methods of the laboratory diagnosis of campylobacter infection, including the main principles of the identification of campylobacter species, are presented. the possibility of using the enzyme immunoassay for the serological diagnosis of campylobacter infection is sho ...19892741606
identification of campylobacter jejuni and c. coli by gel electrophoresis of the outer membrane proteins.analysis of the electrophoretic profiles of the outer membrane proteins could be used to differentiate campylobacter jejuni (16 strains) from campylobacter coli (10 strains). this observation was confirmed by the study of dna homology obtained by a quantitative filter hybridization method. the hippurate hydrolysis test gave a poor correlation with the results of differentiation obtained by dna homology studies and outer membrane protein profile.19892745680
semisolid blood-free selective-motility medium for the isolation of campylobacters from stool specimens.isolation of campylobacter jejuni and c. coli from stool specimens is done by growing campylobacter colonies on solid selective media with or without blood. however, recognition of these colonies can be difficult. therefore, we decided to evaluate an isolation procedure based on the swarming of campylobacters through a semisolid medium. we developed a semisolid blood-free selective motility (ssm) medium which is composed of mueller-hinton broth with 0.4% agar and supplemented with cefoperazone ( ...19892745681
in vitro binding of campylobacter jejuni surface proteins to murine small intestinal cell membranes.surface proteins of nine campylobacter jejuni strains belonging to three different serovia were extracted with lysozyme/ethylenediamine-tetraacetic acid. the preparations bound to isolated murine small intestinal cells and to a membrane fraction (mf) of isolated brush borders obtained by detergent treatment with triton x-100 and nonidet p40. binding was demonstrated by an enzyme-linked immunosorbent assay procedure. using lipopolysaccharide (lps)- and flagella-specific antisera the contribution ...19892747590
campylobacter jejuni antibody in patients with diarrhoea and asymptomatic individuals in lagos, nigeria.seventy patients with acute diarrhoea and sixty asymptomatic children in lagos, nigeria were screened for complement fixing and agglutinating antibodies to campylobacter jejuni in wells of microtitre plates using automatic microtitre pipettes. out of the seventy patients with acute diarrhoea, 55 (78.6%) had complement fixing antibody, while 48 (68.5%) had agglutinating antibody in their sera. twenty-nine (48.3%) and 21 (35%) out of the sixty asymptomatic children screened had complement fixing a ...19892743910
cell surface protein antigen from wolinella recta atcc 33238t.a high-molecular-weight (approximately 150,000) protein was selectively isolated by acid extraction from the cell surface of wolinella recta and purified by negative adsorption on deae-cellulose and gel filtration. sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that this protein was found in w. recta but not in other wolinella species, such as w. curva and w. succinogenes. sera from patients with periodontitis reacted strongly with this protein antigen, whereas sera fr ...19892753998
enterotoxin production and serogroups of campylobacter jejuni and campylobacter coli from patients with diarrhea and from healthy laying hens.enterotoxin production, a possible virulence factor, was determined in campylobacter jejuni and campylobacter coli by two different techniques, the cho cell test and the gm1 enzyme-linked immunosorbent assay. the frequency of enterotoxigenic campylobacter strains was 32% in strains from both humans with acute enteritis and healthy laying hens, as measured by the cho cell test. the cho cell test was significantly more sensitive than the gm1 enzyme-linked immunosorbent assay in the detection of en ...19892754001
virulence of campylobacter jejuni for chicken embryos.the pathogenicity of campylobacter jejuni was examined in chicken embryos. in this system, mortality data and histopathological findings induced by organisms and by bacterium-free filtered broth were identical. the absence in chicken embryo tissues both of organisms and of an inflammatory infiltrate suggests a toxin etiology.19892754005
effects of sample holding time, temperature, and atmosphere on the isolation of campylobacter jejuni from dogs.stool specimens were collected from 39 dogs, inoculated onto campylobacter blood agar plates, and divided into four subsamples. subsamples were held at 4 and 25 degrees c in room air and in a microaerobic environment and were reinoculated at 1, 2, 3, 4, 6, and 8 h. c. jejuni survived at least 3 h when it was held at 4 degrees c, but less than 2 h when it was held at 25 degrees c. the holding atmosphere was not associated with a difference in isolation rates.19892754014
[endocarditis due to campylobacter jejuni and acquired immunodeficiency syndrome]. 19892756206
clinical and immunologic significance of cholera-like toxin and cytotoxin production by campylobacter species in patients with acute inflammatory diarrhea in the usa.the humoral immune response to both campylobacter jejuni cell surface antigens and to potential toxins of the organism was studied in 64 adults with inflammatory diarrhea. in an enzyme-linked immunosorbent assay (elisa) for surface antigens, 17 (71%) of 24 persons with campylobacter enteritis showed seroconversion in more than one immunoglobulin class, versus only 2 (5%) of 40 patients with non-campylobacter enteritis. in a gm1, ganglioside-based elisa for detecting serum igg to cholera-like ent ...19892760498
colonization of mice by campylobacter jejuni.both streptomycin-treated and untreated swiss white mice were irregularly colonized when challenged orogastrically with between 1 and 10(11) viable organisms of either of two strains of campylobacter jejuni. the organisms were occasionally recovered from portions of the intestinal tracts of these animals in numbers ranging from 10(1) to 10(3)/g when the challenge doses were 10(10) or more. when germfree mice were challenged with 10(8) organisms of either strain, the entire intestinal tracts of a ...19892744846
the role of macrophages in experimental campylobacter-infections of mice in lagos, nigeria.the role of macrophages in experimental campylobacteriosis was studied in mice whose macrophages had been blocked using colloidal carbon particles as the blockading agent. two sets of mice received 0.4 ml intraperitoneally and 0.4 ml intravenously of colloidal carbon, respectively, and each set was then inoculated with a clinical isolate of campylobacter jejuni intraperitoneally. the treatment resulted in a lethality of 66.6% in mice given colloidal carbon intraperitoneally and 58.0% in mice giv ...19892763361
immune response to campylobacter jejuni and campylobacter coli in a cohort of children from birth to 2 years of age.a cohort of 111 children from bangui, central african republic, was surveyed for enteric campylobacter infections from birth to the age of 2 years; stools were examined biweekly in these children until 6 months of age and at least four times per year thereafter until 2 years of age and after each diarrheal episode. blood samples were obtained at birth and at 3, 6, 9, 12, 18, and 24 months of age. antibodies against glycine-extracted membrane antigens, purified flagella, and cholera toxin (ct) we ...19892744860
enhancement of campylobacter jejuni virulence by serial passage in chicks.four isolates of campylobacter jejuni were studied to determine changes in virulence following six serial passages in chicks. chicks that received invasive isolates exhibited diarrhea and depressed weight gain. immature mice were used to assess virulence of the passaged isolates of c. jejuni. nine-day-old mice infected with passaged isolates showed lethargy, dehydration, depression, decreased weight gain, and occult blood in feces. mouse pups inoculated with the third and sixth chick passage lev ...19892775092
waterborne outbreak of campylobacter enteritis after outdoors infantry drill in utti, finland.diarrhoea, abdominal pain, malaise and fever affected 75 of the 88 conscript soldiers in utti, finland after an outdoors infantry drill. campylobacter jejuni, heat-stable serotype 3/43/59, was isolated from 37 out of 63 men investigated. a clear serological response was evident in the risk group and negligible in the control group. the entire population at risk was interviewed. the outbreak was associated with the consumption of untreated surface water. c. jejuni, heat-stable serotype 3/43/59, w ...19892776848
[biochemical and serologic characteristics of campylobacter jejuni/coli strains causing diarrhea in children].one hundred strains of campylobacter jejuni/coli isolated from faeces of children with diarrhoea were characterised. frequency of isolation of these microorganisms from faeces of children with enterocolitis symptoms was evaluated. in this group campylobacter jejuni/coli constituted 11.4% of all isolates, being the dominant etiologic agent of these infections. biotype pattern of 100 campylobacter jejuni/coli strains was determined. biotype i c.jejuni prevailed and c. coli constituted as much as 3 ...19892761320
inhibition of enteropathogenic bacteria by human milk whey in vitro.diarrhea caused by enteropathogenic bacteria is a leading cause of childhood mortality world-wide, particularly in less developed regions. breast-feeding has been advocated to protect infants and children from infectious illnesses. we examined the antibacterial activity of human whey in vitro against multiple strains of the following species of enteropathogenic bacteria, campylobacter jejuni, escherichia coli, salmonella typhimurium, shigella flexneri, shigella sonnei and vibrio cholerae, all is ...19892787902
interaction of campylobacter species with antibody, complement and phagocytes.the opsonisation of four different campylobacter species for human neutrophils was studied using a chemiluminescence system and electron microscopy. opsonisation of campylobacter fetus, campylobacter coli, and campylobacter jejuni was mediated by antibody and enhanced by complement. antibody was not, however, required for the phagocytosis of campylobacter pylori because it activates the classical pathway of complement directly. this unusual property may be important in the pathogenesis of c pylo ...19892788111
[antibodies to campylobacter jejuni in patients with acute enteritis].in 233 patients with acute diarrhoea in paired sera campylobacter antibodies classes igg and iga were assessed by the elisa method. as antigen the external membrane protein of the strain campylobacter jejuni was used. raised igg levels (greater than 50 u.) and/or iga (greater than 80 u.) were found in 15% of all examined patients. a quadruple increase of values in one or both classes was recorded in 12.4% of the patients. the incidence of antibodies against campylobacter jejuni provides evidence ...19892790981
comparative in vitro activity of lomefloxacin, a difluoro-quinolone.lomefloxacin is a new difluoro-quinolone. in this study, we have determined the in vitro activity of lomefloxacin against a wide range of clinical bacterial isolates and compared it with that of other fluoro-quinolones and some unrelated antimicrobials. lomefloxacin was very active against enterobacteriaceae (mic90, 0.5 micrograms/ml) with activity comparable to that of ofloxacin (mic90, 0.25 micrograms/ml). lomefloxacin was moderately active against isolates of pseudomonas aeruginosa (mic90, 4 ...19892791500
the gnotobiotic piglet as a model for the pathogenesis of campylobacter jejuni infection.the pathogenesis of enteric changes was studied in gnotobiotic piglets which, after hysterectomy had been infected orally with campylobacter jejuni on the first day of their life. the involvement of the entire large intestine became clinically manifest by scouring on days post infection (dpi) 4 to dpi 5, and pathomorphologically, by simultaneous inflammation and severe edema of the intestinal wall. histology and sem revealed inflammatory edema with abundant neutrophils, microulcerations, focal p ...19892765093
microbial etiology of acute gastroenteritis in hospitalized children in kuwait.during a period of 15 months 621 hospitalized children with acute gastroenteritis and 152 control children were investigated for etiologic agents of the disease. putative enteropathogens were identified in 86% of the patients and 10% of the controls. common viral agents associated with gastroenteritis among children included rotaviruses (45%) and enteric adenoviruses (4%). bacterial pathogens infecting children were salmonella serotypes (24%), enterotoxigenic escherichia coli (9%), campylobacter ...19892797954
aeromonas-associated diarrhea in filipino children.aeromonas was isolated from the stools of ten out of sixty-six young (15%) suffering from acute diarrhea. these are the first reported cases in the philippines and the prevalence cited was higher than reported elsewhere. three species isolated were a. hydrophilia (50%), a. caviae (30%) and a. sobria (20%), either singly (60%) or in combination with etec and campylobacter jejuni. mean age of patients was 12.9 months with mean pre-admission duration of diarrhea of 48.6 hours. mean stool frequency ...19892798995
inhibition of campylobacter jejuni in vitro by broiler chicken caecal contents. 19892800258
in vitro activities of lomefloxacin and temafloxacin against pathogens causing diarrhea.the in vitro activities of temafloxacin (a63004) and lomefloxacin (sc-47111; ny-198) were compared with those of seven other antibiotics against 146 isolates of bacterial enteric pathogens, including campylobacter jejuni and campylobacter coli. ciprofloxacin was the most active drug against the salmonella, shigella, yersinia, and vibrio spp. tested. lomefloxacin, temafloxacin, and difloxacin were the most active drugs tested against campylobacter spp. (mic for 90% of strains, 0.125 to 0.25 micro ...19892802563
natural bactericidal activity of human serum against strains of campylobacter jejuni isolated in lagos, nigeria.the sensitivity of campylobacter jejuni strains la4, la12, la36, la53, la1214, la1308 and la1309 constituting the most prevalent strains of c. jejuni isolated in lagos, nigeria to the bactericidal activity of normal human serum was determined using microtiter technique. results obtained shows that only strains la 36 and la53 were serum resistant while others (strains la4, la12, la1214, la1308 and la1309) were serum sensitive. this finding is important epidemiologically and is the first report on ...19892805054
in-vitro activity of meropenem against clinical isolates obtained in canada.the in-vitro activity of meropenem, a new parenteral carbapenem, was compared with that of imipenem, ceftazidime, cefotaxime, piperacillin, gentamicin and, where appropriate, other antibiotics against recent clinical isolates and characterized beta-lactamase producers. mics were determined by a standard agar dilution procedure and two inocula (10(4) and 10(6) cfu) were used throughout. meropenem inhibited 90% of isolates of escherichia coli, klebsiella pneumoniae, proteus mirabilis, indole-posit ...19892808216
comparative in vitro activity of ceftibuten (sch 39720) against bacterial enteropathogens.ceftibuten is a new orally active cephalosporin with significant bioavailability. its in vitro activity was compared with those of other agents against 383 strains of enteric pathogens derived from clinical specimens. ceftibuten was very active against the strains of the family enterobacteriaceae tested (overall mic for 90% of strains tested, 0.25 microgram/ml) but was less active against campylobacter jejuni (mic for 90% of strains, 16 micrograms/ml). the mbc was one to two dilutions higher tha ...19892751289
etiology of acute childhood diarrhoea in calcutta.of the 152 cases of acute diarrhoea, 124 (81.5%) revealed potential pathogens. altogether 27 (21.2%) out of 127 strains of escherichia coli, klebsiella pneumoniae, enterobacter, proteus and acinetobacter produced enterotoxin. single pathogenic bacteria (40 cases 26.3%), parasite (6; 6%), rota virus (6; 6%), toxigenic bacteria (19; 12.5%) and mixed agents (37; 24.24.3%) were recorded in 108 cases (71.0%). another 14 (9.2%) cases exclusively revealed moderate to heavy growth of suspected enteric p ...19892815324
[dynamics of campylobacter jejuni invasion in gnotobiotic piglets].the study deals with the results of the investigation of c. jejuni populations in the intestines of gnotobiotic piglets after oral infection with c. jejuni in the span of one to five days after infection (dai). the infection of the whole large intestine was revealed, with the onset of pathological lesions 3rd dai. c. jejuni was isolated from liver, gallbladder, ileum, rectum, colon, mesenterial lymphatic node, rectal smears and blood. from 1st to 5th dai c. jejuni was demonstrated in liver, gall ...19892815491
early treatment of campylobacter jejuni enteritis.the bacteriologic and clinical effects of early antibiotic treatment of campylobacter jejuni enteritis were studied. erythromycin rapidly eliminated c. jejuni from stools, whereas trimethoprim-sulfamethoxazole did not. despite its bacteriologic effectiveness, erythromycin did not reduce the duration or severity of diarrhea, abdominal pain, or other symptoms.19892818711
[survey of campylobacter jejuni/coli infection and risk factors in children].from march to may 1987, 798 children under 7 years of the outpatients in chengdu district were examined and their stool samples cultured for campylobacter jejuni/coli. c. jejuni/coli was isolated from 31 (9.51%) of 326 children with diarrhea and 22 (4.66%) of 472 children with non-diarrhea. the difference was significant (p less than 0.05). this result indicated that c. jejuni/coli is an important pathogen of children diarrhea in chengdu district. meanwhile, by inquiry we investigated risk facto ...19892793148
cloning and expression of a tetracycline resistance determinant from campylobacter jejuni in escherichia coli.the tetracycline resistance gene (tet) from the campylobacter jejuni plasmid pfkt1025 was cloned into both puc18 and pbr322 and was expressed when the chimeric plasmids were introduced into escherichia coli. the location of the tet determinant on the chimeric plasmids was determined by bal 31 deletion mapping within a 2.25-kilobase (kb) rsai-hincii fragment. a protein of approximately 70 kilodaltons was consistently produced by e. coli maxicells harboring the cloned tet determinant. a 500-base-p ...19872823694
genomic rearrangements associated with antigenic variation in campylobacter coli.campylobacter coli and campylobacter jejuni share a limited number of highly conserved dna sequences with members of the family enterobacteriaceae. one of these sequences was cloned from c. coli vc167, and the region of homology to the enteric sequences was determined to be confined to a 700-base-pair region. the dna represented in this clone undergoes a programmed, reversible rearrangement in vc167 that is associated with flagellar antigenic variation.19882826396
[rotavirus infection in the older child and adult].rotaviruses were detected in 1.3% of more than 10,000 stool samples from children--older than 6 years--and adults. beside rotaviruses the main enteropathogenic agents found were salmonellae (6.3%) and campylobacter jejuni/coli (1.7%). similar as the rotavirus infection of infants, in adults symptoms and signs were nearly the same except that the disease was not so severe. a correlation of the rotavirus infection with certain behaviour or eating habits was not found.19872830184
gene disruption and replacement as a feasible approach for mutagenesis of campylobacter jejuni.campylobacter jejuni and campylobacter coli are important causes of human enteric infections. several determinants of pathogenicity have been proposed based on the clinical features of diarrheal disease and on the phenotypic properties of campylobacter strains. to facilitate an understanding of the genetic determinants of campylobacter virulence, we have developed a method for constructing c. jejuni mutants by shuttle mutagenesis. in the example described here, a kanamycin resistance gene was in ...19882832375
nucleotide sequence analysis and expression of a tetracycline-resistance gene from campylobacter jejuni.resistance to tetracycline in the microaerophilic gram-negative bacterium campylobacter jejuni is plasmid-mediated. a 6.9-kb hindiii dna fragment containing the tetracycline-resistance (tcr) gene (designated teto) from the c. jejuni conjugative plasmid pua466 was cloned into puc8, and the resultant plasmid puoa1 was used to transform escherichia coli to tc resistance. the teto gene was localized at a 2.0-kb region comprising 0.2-kb and 1.8-kb hincii fragments, and the nucleotide sequences were d ...19882836268
the relationship between intestinal campylobacter species isolated from animals and humans as determined by brenda.intestinal thermophilic campylobacter species produce stable patterns when subjected to bacterial restriction endonuclease dna analysis (brenda); this technique is therefore of considerable value in epidemiological studies. brenda was used to examine thermophilic campylobacter species from humans, wild and domestic animals. one hundred and ninety-four (61%) of 316 isolates of campylobacter jejuni from humans had brenda patterns which could be matched to those of animal isolates. poultry appear t ...19882837404
severe guillain-barré syndrome: an association with iga anti-cardiolipin antibody in a series of 92 patients.to determine whether anti-cardiolipin antibodies (aca) are associated with acute guillain-barré syndrome (gbs), we studied sera from 92 patients with gbs, 82 age- and sex-matched hospital controls and 24 patients with uncomplicated cytomegalovirus or campylobacter jejuni infection, using an isotype-specific aca enzyme-linked immunosorbent assay (elisa). iga aca titres (but not igg aca or igm aca) were significantly elevated (p = 0.002) in gbs patients compared to controls, and associated with pe ...19882840454
investigation of zoonotically acquired campylobacter jejuni enteritis with serotyping and restriction endonuclease dna analysis.five strains of campylobacter jejuni were isolated from asymptomatic coyotes being maintained in a vivarium. according to the results of serotyping with thermostable antigens and of bacterial chromosomal restriction endonuclease analysis, one of the c. jejuni strains isolated from the coyotes was responsible for enteritis in a laboratory animal technician providing husbandry for the laboratory-maintained coyotes.19892808666
study of plasmid screening amongst pathogenic bacteria isolated in nigeria.clinical isolates of neisseria gonorrhoeae, campylobacter jejuni, escherichia coli, shigella dysenteriae, shigella boydii, yersinia spp. and salmonella spp. were screened for the presence of plasmids. approximately 80% of these strains harboured plasmids ranging in molecular weight from 1.0 to 45 x 10(6) daltons.19882845756
a new heat-labile cytolethal distending toxin (cldt) produced by campylobacter spp.a new heat-labile toxin cytolethal to cho, vero, hela, and hep-2 cells and negative in y-1 cells has been demonstrated in culture filtrates of many strains of campylobacter spp. this new toxin was termed a cytolethal distending toxin (cldt) to reflect the progressive cell distention and eventual cytotoxicity observed in all sensitive tissue cells. cldt was distinct from previously reported cytotoxins and cholera-like enterotoxin produced by some campylobacter spp. since cho elongation induced by ...19882849028
rotaviruses in infants with diarrhea studied by viral rna electrophoresis in ankara, turkey.incidence of rotavirus in children under 2 years of age, admitted to 2 children's hospitals i n ankara, turkey, from july 1984-june 1985 was measured using viral rna electrophoresis in agarose gels. 375 children were selected at random from over 14,000 diarrhea admissions, and their stool specimens taken on admission were compared to those of 333 children without diarrhea. 61 (16.3%) of the diarrhea cases had detectable rotavirus, compared to 0% in controls. bacteriologic tests for salmonella ...19872851886
in vitro activity of azithromycin (cp-62,993), a novel macrolide, against enteric pathogens.azithromycin (cp-62,993 [9-deoxy-9a-methyl-9a-aza-9a-homoerythromycin]) is a novel macrolide antimicrobial. in this study the in vitro activity of cp-62,993 has been determined against selected enteropathogens, including clostridium difficile, and compared with that of erythromycin. mics were determined using an agar incorporation technique in mueller-hinton medium, containing saponin-lysed horse blood at a final concentration of 10% v/v, with an inoculum of 10(4) cfu. cp-62,993 was considerably ...19882854515
polymicrobial aetiology of travellers' diarrhoea.of 35 us peace corps volunteers in thailand, 20 (57%) had a total of 30 episodes of diarrhoea during their first 6 weeks in the country. enteric pathogens were associated with 90% of the episodes. a single pathogen was identified in 17 (57%) episodes, 2-4 pathogens were identified in 10 (33%) episodes, and there were 15 symptomless infections. enterotoxigenic escherichia coli (etec) was identified in 37% of these episodes, and various salmonella serotypes were isolated in 33%. infections with 9 ...19852857430
cytotoxic activity on chinese hamster ovary cells in culture filtrates of campylobacter jejuni/coli. 19852863540
isolation and characterization of catalase-negative and catalase-weak strains of campylobacter species, including "campylobacter upsaliensis," from humans with gastroenteritis.during 1987 and 1988, nine strains of catalase-negative or -weak campylobacter species were isolated in alberta, canada. dna hybridization studies demonstrated that seven strains were "campylobacter upsaliensis," one strain was highly homologous with campylobacter jejuni dna, and one strain was a campylobacter unrelated to the other two species. all "c. upsaliensis" strains were hippurate negative, and six of seven were susceptible to cephalothin. the unusual variant of c. jejuni was hippurate p ...19892778067
identification and characterization of two campylobacter jejuni adhesins for cellular and mucous substrates.campylobacter jejuni is able to colonize the human intestinal mucosa and cause disease. for this reason, it was important to investigate mechanisms by which c. jejuni adheres to epithelial cells and intestinal mucus gel. all strains of c. jejuni used were able to adhere to int 407 epithelial cells and mucus, but high adherence to one substrate did not necessarily indicate comparable adherence to the other. the adherence of c. jejuni to cells was inhibited partially by treating the bacterial cell ...19862873103
nosocomial outbreak of campylobacter jejuni meningitis in newborn infants.in a nosocomial outbreak of campylobacter jejuni infection 11 newborn infants (7 female, 4 male) had meningitis. the outbreak was caused by a single strain of c jejuni, as demonstrated by biotyping (biotype i), serotyping (lau 7/pen 18 on heat-stable antigens, a new serotype on heat-labile antigens), and the identical susceptibility pattern and outer-membrane-protein profile on sodium dodecyl sulphate/polyacrylamide gel electrophoresis. specific antibodies against the outbreak strain (enzyme-lin ...19862873408
restriction endonuclease digest patterns of chromosomal dna from nitrate-negative campylobacter jejuni-like organisms.high molecular weight chromosomal dna was isolated from ten nitrate-negative campylobacters of human origin (gastric biopsies and faecal specimens). the dna was digested with various restriction endonucleases and the patterns obtained were compared with those of campylobacter coli, c. fetus subsp. fetus, c. jejuni and c. laridis reference strains. electrophoresis of hae iii digests of dna in 0.5% agarose gave excellent patterns which comprised at least 25 well-resolved bands with fragment sizes ...19852876913
prevalence of various enteropathogens in the feces of diarrheic and healthy calves.the presence of various enteropathogens was examined in the feces of homebred dairy calves reared in a restricted geographical area of france (north west of county of indre-et-loire) during winter 1983-1984. two distinct surveys were carried on: a case-control study including 32 diarrheic calves and 21 healthy calves bred in 53 different farms; and a separate study on nine diarrheic calves in another farm. the following infectious agents were looked for, by specific methods of detection: escheri ...19862877609
substrate utilization by campylobacter jejuni and campylobacter coli.an attempt was made to elucidate in campylobacter spp. some of the physiologic characteristics that are reflected in the kinetics of co2 formation from four 14c-labeled substrates. campylobacter jejuni and c. coli were grown in a biphasic medium, and highly motile spiral cells were harvested at 12 h. of the media evaluated for use in the metabolic tests, minimal essential medium without glutamine, diluted with an equal volume of potassium sodium phosphate buffer (ph 7.2), provided the greatest s ...19862877615
nucleotide sequence analysis of tetracycline resistance gene teto from streptococcus mutans dl5.streptococcus mutans dl5, isolated from the dental plaque of a pig, was resistant to high levels of streptomycin (sm, 20 mg/ml), erythromycin (em, 1 mg/ml), and tetracycline (tc, greater than 100 micrograms/ml), but contained no detectable plasmid dna. the smr and emr determinants were cloned from cellular dna on the self-replicating 5-kilobase-pair (kbp) ecori fragment of pam beta 1 and the 4.2-kbp cryptic plasmid pva380-1, respectively, by transformation of streptococcus sanguis challis. helpe ...19882841293
urine and faecal iga response during naturally acquired infection with campylobacter jejuni. 19872883461
raw beef, pork and chicken in japan contaminated with salmonella sp., campylobacter sp., yersinia enterocolitica, and clostridium perfringens--a comparative study.one hundred and twenty samples each of raw ground beef, pork and chicken from ten local grocery stores in shimane prefecture, japan, were examined for the presence of salmonella sp. (sal), campylobacter jejuni (cj), campylobacter coli (cc), yersinia enterocolitica (ye), and clostridium perfringens (cp) from april 1984 to march 1985. a total of 205 isolates of sal (112 strains), cj (64 strains), cc (one strain), ye (7 strains) and cp (21 strains) were recovered from 17 beef (14.2%), 31 pork (25.8 ...19872887078
hospital epidemic of neonatal campylobacter jejuni infection. 19852865631
an outbreak of campylobacter jejuni infection in a neonatal intensive care unit. 19882895144
gene transfer from escherichia coli to campylobacter species: development of shuttle vectors for genetic analysis of campylobacter jejuni.a shuttle cloning vector (pil550) has been constructed which can be mobilized from escherichia coli to campylobacter jejuni, campylobacter coli, and campylobacter fetus by complementation with the transfer functions of an incp plasmid in trans, with a frequency of 10(-4) transconjugants per donor. we also present evidence for a dna modification system in c. jejuni.19872822671
campylobacter jejuni infection as a hospital problem: an overview. 19882896736
clinical comparison of campylobacter jejuni and c coli diarrhoea. 19882893006
comparison of preservation media and freezing conditions for storage of specimens of faeces.to evaluate the long-term recoverability of bacterial enteropathogens, two freezing conditions (deep-freezing at -70 degrees c and liquid nitrogen) and three preservation media (cary-blair, amies, and buffered glycerol-saline) were tested. these were compared with storage in containers with no preservation medium and refrigeration at 4 degrees c. at 4 degrees c, viability of organisms could not be consistently maintained beyond one month; cary-blair medium generally gave the best results and sto ...19892915366
an outbreak of campylobacter jejuni infection in a neonatal intensive care unit.during a period of 2 weeks campylobacter jejuni infection was diagnosed in seven infants in a neonatal intensive care unit. an identical serotype of camp. jejuni was found in five of the cases. investigations suggested that a common source of infection or infection acquired from mothers during delivery were unlikely. the dates of onset and the common serotype indicate that the outbreak may have been caused by person-to-person spread.19872880901
clinical characteristics of campylobacter jejuni and c coli enteritis. 19882895866
[campylobacter jejuni infections in the netherlands]. 19892919393
campylobacter jejuni isolations from mexican and swedish patients, with repeated symptomatic and/or asymptomatic diarrhoea episodes.the presence of different campylobacter jejuni serotypes in swedish patients with diarrhoea and in mexican patients with or without diarrhoea was investigated with special reference to repeated isolations during the course of infection and to symptomatic and asymptomatic episodes. the study included 136 c. jejuni isolates from 62 mexican children and 173 isolates from 68 swedish patients. the bacteria were serotyped for heat-stable (hs) and heat-labile (hl) antigen. swedish patients, all with sy ...19892917617
mucus colonization as a determinant of pathogenicity in intestinal infection by campylobacter jejuni: a mouse cecal model.human isolates of the intestinal pathogen campylobacter jejuni have been shown to colonize mucus on the outer surface and deep within the intestinal crypts of gnotobiotic or germfree mice. the cecal crypts are preferentially colonized. a model of mucus colonization by c. jejuni in the mouse cecum has been developed, using antibiotic- and magnesium sulfate-treated specific-pathogen-free animals. these spiral-shaped bacteria colonize the mucus in a similar manner to the normal spiral-shaped microb ...19862935499
[yersinia enterocolitica and campylobacter jejuni in their significance for milk hygiene]. 19852931637
in-vitro activity of newer quinolones against aerobic bacteria.nalidixic and five newer 4-quinolones, ciprofloxacin, enoxacin, norfloxacin, ofloxacin and pefloxacin were tested against 576 recent clinical aerobic bacterial isolates. the 4-quinolones were regularly active (mic90 less than 4 mg/l) against the following bacteria: staphylococcus aureus, s. epidermidis, s. saprophyticus, different enterobacteriaceae, haemophilus influenzae, campylobacter jejuni, pseudomonas aeruginosa, agrobacter spp., aeromonas spp., plesiomonas spp., neisseria meningitidis. ot ...19862940214
[campylobacter jejuni enteritis associated with hemolytic-uremic syndrome]. 19862940534
the comparative in vitro activity of twelve 4-quinolone antimicrobials against enteric pathogens.the minimal inhibitory concentrations (mics) of twelve 4-quinolone antimicrobials were determined for salmonella typhi (25), salmonella spp. (50), shigella spp. (50), campylobacter jejuni (100), vibrio cholerae (10), vibrio parahaemolyticus (10), yersinia enterocolitica (25), aeromonas hydrophila (25) and plesiomonas shigelloides (10). mics were determined using an agar dilution technique in mueller-hinton agar (oxoid, england) supplemented with 10% lysed horse blood. antibiotic containing plate ...19852941257
ciprofloxacin in the treatment of acute bacterial diarrhea: a double blind study.in a double-blind, randomized, placebo-controlled trial 50 adult patients with acute diarrhea received either 500 mg ciprofloxacin b.i.d. or a placebo for five days. results were evaluated in 21 patients in the ciprofloxacin group (10 with salmonella spp., 11 with campylobacter jejuni) and 25 patients in the placebo group (16 with salmonella spp., 5 with campylobacter jejuni, 4 with shigella spp.). the duration of fever in patients treated with ciprofloxacin was 1.5 days versus 2.3 days in the p ...19862941290
campylobacter jejuni and campylobacter coli diarrhoea in rural and urban populations in yugoslavia.during a 4-month period during the summer of 1985, campylobacters were isolated from 338 (16.3%) of 2080 patients with acute diarrhoea attending the university hospital of infectious diseases in zagreb. of these isolates 220 (64.1%) were campylobacter jejuni and 118 (34.9%) were c. coli. the patients were drawn from three residential zones in and around zagreb: inner city, peripheral city and rural. incidences of campylobacter diarrhoea ranged from 71 per 100,000 per year in inner city residents ...19892917618
biochemical and serological characteristics of campylobacter jejuni strains isolated from chickens. 19862953191
comparative in vitro activity of a-56268 (te-031) against gram-positive and gram-negative bacteria and chlamydia trachomatis.the in vitro activity of a-56268 (te-031) was determined and compared with that of 13 antibiotics against 401 gram-positive and gram-negative bacteria and 11 strains of chlamydia trachomatis. a-56268 was very active against methicillin-susceptible staphylococcus aureus and neisseria gonorrhoeae, and was among the most active of the agents tested against listeria monocytogenes, streptococci and chlamydia trachomatis. it was moderately active against haemophilus spp., vibrio spp., campylobacter je ...19872954818
campylobacters and impaired fetal development in mice.intravenous injection of eight human strains of campylobacter fetus ss fetus and campylobacter jejuni into mice at various stages of pregnancy demonstrated significant strain differences in ability to affect implantation of the fertilised ovum and to cause resorption of the mouse fetus. implantation was significantly impaired when c. fetus ss fetus was injected intravenously on day 2 of pregnancy, but no effect was observed in mice receiving c. jejuni. on day 6 of pregnancy, before the developme ...19882961890
in vitro susceptibility of diarrhoea producing gram negative enteric bacteria to sulfasalazine, 5-aminosalicylic acid, sulfapyridine and four quinolones. brief report.the in vitro susceptibility of diarrhoea producing gram negative enteric bacteria to sulfasalazine, 5-aminosalicylic acid, sulfapyridine and four quinolones was investigated using an agar dilution method. all strains were resistant to 1600 micrograms/ml of sulfasalazine and 5-aminosalicylic acid. mic range of sulfapyridine for y. enterocolitica was 3.1-25 micrograms/ml (median:6.2) and for salmonella 25-100 micrograms/ml (median: 100) campylobacter jejuni/coli were less susceptible to sulfapyrid ...19882899438
[enterotoxigenic escherichia coli and campylobacter jejuni in acute diarrheic syndrome in chilean infants]. 19882965884
[personal experience with the epidemiology of infections caused by campylobacter jejuni]. 19862937545
comparative in-vitro activities of a-56268 (te-031) and erythromycin against 306 clinical isolates.the inhibitory (mic) and bactericidal (mbc) activities of a new macrolide a-56268 (te-031) against 306 clinical aerobic bacterial isolates was compared with that of erythromycin. the mic90/mbc90 ratios for a-56268 were: campylobacter jejuni 4/16, haemophilus influenzae 8/8-16, h. parainfluenzae 8/8-16, legionella pneumophila 0.06/0.5, methicillin-sensitive isolates of staphylococcus aureus 0.5/1, and coagulase negative staphylococci 1/8, methicillin resistant isolates of staph. aureus and coagul ...19882968968
influence of iron on growth, morphology, outer membrane protein composition, and synthesis of siderophores in campylobacter jejuni.three human isolates of campylobacter jejuni were grown in a biphasic culture medium with and without the addition of a synthetic chelator to induce iron limitation. cells grown in low-iron medium exhibited slower growth rates and altered cellular morphology. increased numbers of longer, more filamentous forms were seen in gram-stained smears. three proteins, with apparent mrs of 82,000, 76,000, and 74,000, were consistently present in the outer membrane of cells grown in low-iron medium. at lea ...19862944843
[effect of nacl on the survival of campylobacter jejuni at a temperature of 20 degrees centigrade]. 19872981001
survey of plasmids and resistance factors in campylobacter jejuni and campylobacter coli.a total of 688 isolates of campylobacter jejuni and campylobacter coli were screened for the presence of plasmid dna by agarose gel electrophoresis and were tested for susceptibility to ampicillin, chloramphenicol, erythromycin, streptomycin, and tetracycline. of the isolates examined, 32% were noted to harbor plasmid dna, ranging in size from 2.0 to 162 kilobases. only tetracycline resistance was noted to correlate with the presence of plasmids. plasmids capable of transferring tetracycline res ...19852984981
dna relatedness and biochemical features of campylobacter spp. isolated in central and south australia.investigations of the etiology of diarrhea in patients in south australia and the northern territory showed that campylobacter spp. other than campylobacter jejuni and c. coli were common in children. campylobacters which were hippurate positive, nitrate negative, and susceptible to cephalothin and polymyxins were shown to be closely related to c. jejuni by dna studies. thermotolerant catalase-negative campylobacters were also isolated. these were h2s negative and biochemically resembled the cat ...19852991331
in vitro susceptibility of campylobacter jejuni to 27 antimicrobial agents and various combinations of beta-lactams with clavulanic acid or sulbactam.the in vitro susceptibility of human isolates of campylobacter jejuni was investigated with 27 antibiotics and 8 combinations of beta-lactams with clavulanic acid or sulbactam. ansamycin, the new quinolines, erythromycin, and cefpirome were the most active drugs against c. jejuni; amoxicillin, ampicillin, cefotaxime, and ceftazidime 90% of the isolates, greater than or equal to 50 mg/liter). the activity of various beta-lactams was unchanged by the addition of clavulanic acid or sulbactam.19852994557
diarrheal illness among infants and toddlers in day care centers. i. epidemiology and pathogens.we conducted a 2-year prospective study of diarrheal illness in children ages 0 to 36 months in 22 day care centers in maricopa county, arizona. in 7464 child-months of observation, 465 sporadic cases and 170 outbreak-associated cases of diarrhea were identified. enteric pathogens were identified in 20% of diarrhea episodes. giardia lamblia, rotavirus, and campylobacter jejuni were the most common pathogens. giardia was significantly more common in toddlers than in infants and was found in 19% o ...19852995628
[epidemiology, etiology and laboratory diagnosis of infectious diarrhea diseases in the tropics].diarrhoeal diseases belong to the leading causes of morbidity and mortality in tropical countries, especially in infants and small children. about one billion episodes are estimated for this group of age with 4.6 million fatalities. many causes are discussed to explain the high incidence: bottle feeding of infants, protein malnutrition, unsafe drinking water and unsafe disposal of excrements and sewage, unsufficient consciousness of personal and domestic hygiene, lack of knowledge on the origin ...19853000920
the ultrastructure and atpase nature of polar membrane in campylobacter jejuni.polar membrane in campylobacter jejuni has been visualized on membrane vesicles. it was composed of doughnut-shaped particles 5-6 nm in diameter, with stalks, arranged in a hexagonal array. this structure was stabilized on the membrane by a high ionic strength buffer in the presence of 2-mercaptoethanol. histochemical staining indicated localized atpase activity at the poles of the cells. an atpase with distinctive properties has been isolated and purified from this organism; it gives a specific ...19882974756
plasmid-mediated tetracycline resistance in campylobacter jejuni: expression in escherichia coli and identification of homology with streptococcal class m determinant.plasmid pua466, a 45-kilobase transmissible tetracycline resistance plasmid from campylobacter jejuni was mapped with avai, avaii, bcli, hincii, psti, xhoi, and xbai. the resistance determinant was cloned and expressed in escherichia coli and was homologous with a class m determinant from streptococcus spp.19863005233
enteropathogens associated with acute diarrhea in hospitalized infants.thirty-five infants of low socioeconomic status who were living in urban santiago were hospitalized for acute diarrhea were prospectively evaluated for the presence of enteropathogens associated with the episode. some degree of malnutrition was evident in 20 infants (57.1%); 15 of these (75%) were under 6 months of age. mean duration of the hospital stay was 11.8 days for well-nourished patients and 15.7 days for the malnourished patients. one or more enteropathogens were found in 60% of the cas ...19863007718
comparative in-vitro activity of a-56268 (te-031), a new macrolide antibiotic.the comparative in-vitro activity of a-56268 (te-031), a new semisynthetic macrolide antibiotic, was assessed against approximately 400 bacterial isolates. the new drug demonstrated excellent activity against penicillin-susceptible streptococci (mic90s less than or equal to 0.06 mg/l) and methicillin-susceptible staphylococci (mic90 = 0.25 mg/l). among other gram-positive organisms tested, a significant number were resistant to a-56268 as well as to erythromycin and clindamycin. a-56268 was at l ...19872962974
pathogens that cause travelers' diarrhea in latin america and africa.with the advent of rapid and convenient means of transportation, millions of persons travel each year from industrialized to developing countries in the tropics and subtropics. these travelers are at risk for a variety of infectious diseases that are endemic in these areas; the most frequently occurring of these is diarrhea. studies of groups of travelers to latin america and africa have found that approximately one-half develop diarrhea during their stay abroad. etiologic investigations of thes ...19863014633
an epidemiological study of selected calf pathogens on holstein dairy farms in southwestern ontario.fecal samples from calves on 78 randomly selected holstein dairy farms in southwestern ontario were screened for salmonella, campylobacter jejuni/coli, enteropathogenic escherichia coli, rotavirus and coronavirus. based on the observed prevalence, 22% of farms had calves infected with salmonella, 13% with campylobacter jejuni/coli, 41% with enteropathogenic e. coli, 19% with rotavirus and 5% with coronavirus. these estimates can be modified, using a method developed by mullen and prost (1983) fo ...19863017528
cytotoxic and cytotonic factors produced by campylobacter jejuni, campylobacter coli, and campylobacter laridis.complete toxigenicity studies were performed on 341 strains of campylobacter spp., including 23 nonhuman isolates. toxin profiles based on both cytotonic and cytotoxic factors were determined after analyzing responses in vero, hela, cho and y-1 cells. suckling mouse assays were consistently negative for all culture filtrates tested. toxin-producing strains were frequently encountered among both the human and nonhuman strains of campylobacter jejuni, c. coli, and c. laridis investigated. strains ...19863018039
comparison between children treated at home and those requiring hospital admission for rotavirus and other enteric pathogens associated with acute diarrhea in melbourne, australia.the etiology of acute diarrhea in children less than 42 months of age attending one pediatric hospital in melbourne, australia, was studied during a 7-month period encompassing the winter of 1984. pathogens identified in 157 children treated as outpatients with mild disease were compared with those in 232 children hospitalized with severe disease. the pathogens (and frequencies among outpatients and inpatients, respectively) detected were rotaviruses (32.5 and 50.9%), enteric adenoviruses (8.9 a ...19863020082
genetic studies of kanamycin resistance in campylobacter jejuni.campylobacter jejuni 3h40 and 4b20 harbored 59-kilobase (kb) self-transmissible plasmids encoding resistance to kanamycin and tetracycline. although the two antibiotic resistances were more frequently inherited together, some transconjugants and ethidium bromide segregants which were resistant to only one of these antibiotics were recovered. the kanamycin-susceptible, tetracycline-resistant segregants carried plasmids 4 kb smaller than the 59-kb plasmids of their parents, whereas the kanamycin-r ...19863021049
characterization and expression of a cloned tetracycline resistance determinant from campylobacter jejuni plasmid pua466.a tetracycline resistance (tcr) determinant previously cloned from the campylobacter jejuni plasmid pua466 (d. e. taylor, j. bacteriol. 165:1037-1039, 1986) was localized by restriction endonuclease mapping, subcloning, and tn1000 insertion mutagenesis to a 2-kilobase region consisting of 1.8- and 0.2-kilobase hincii fragments. tcr encoded by the cloned fragment (puoa1) was expressed constitutively in escherichia coli. a protein with an apparent molecular weight of 68,000 encoded by puoa1 was pr ...19873036765
antimicrobial resistance of campylobacter jejuni and campylobacter coli with special reference to plasmid profiles of japanese clinical isolates.a total of 111 clinical isolates of campylobacter jejuni and 10 clinical isolates of campylobacter coli were characterized by their susceptibility to nine antimicrobial agents and by their plasmid profiles on agarose gel electrophoresis. all of the c. jejuni isolates were susceptible to chloramphenicol, ciprofloxacin, erythromycin, kanamycin, and nalidixic acid, but 55% were tetracycline resistant. in the 10 c. coli isolates, a high prevalence of multiple-antibiotic resistance was noted. plasmid ...19873038005
Displaying items 501 - 600 of 12694