TitleAbstractYear(sorted ascending)
serotyping of campylobacter fetus subsp. fetus and c. fetus subsp. venerealis by passive hemagglutination technique based on soluble autoclaved antigens.antigens for sensitization of sheep erythrocytes were released from c. fetus subsp. fetus and c. fetus subsp. venerealis suspensions by autoclaving in alkaline and neutral saline and by boiling in alkaline but not in neutral saline. serotyping with rabbit antisera showed the same two serotypes of o-antigens, a and b, as shown by tube agglutination of heated bacteria. all c. fetus subsp. venerealis strains had the a antigen. the c. fetus subsp. fetus strains tested had either the a or b antigen. ...19863751579
campylobacter infection associated with raw milk. an outbreak of gastroenteritis due to campylobacter jejuni and thermotolerant campylobacter fetus subsp fetus.raw milk is identified with increasing numbers of outbreaks of gastroenteritis and is an important vehicle for transmission of campylobacter infection. unlike campylobacter jejuni, campylobacter fetus subsp fetus has not been associated with common-source outbreaks of gastroenteritis. this report describes an outbreak of gastroenteritis involving c jejuni and a thermotolerant strain of c fetus subsp fetus associated with raw milk. fifteen (39%) of 38 persons who attended a banquet in wisconsin i ...19863753617
strain characterization and grouping of campylobacter jejuni and campylobacter coli by interaction with lectins.strains of campylobacter jejuni and campylobacter coli were characterized and grouped by their distinct reaction patterns with lectins. heating of the campylobacter cultures to 100 degrees c and holding for 30 to 60 min greatly enhanced their reactivity with lectins and permitted the grouping of all but 3 of 155 cultures tested in this study without interference of autoagglutination and other nonspecific activities. the lectin reaction patterns of the heated cultures were stable and reproducible ...19863754264
the chicken embryo as a model for campylobacter invasion: comparative virulence of human isolates of campylobacter jejuni and campylobacter coli.eleven-day-old chicken embryos were used to compare the relative virulence of minimally passaged human isolates of campylobacter jejuni and campylobacter coli. graded doses of bacteria were inoculated onto the chorioallantoic membrane, and 50% lethal doses were calculated at 72 h postinfection. strains varied markedly in their ability to invade the chorioallantoic membrane and kill the embryos. the 50% lethal doses varied by about 6 logs for 25 strains of c. jejuni, and by 2 logs for 5 strains o ...19863759232
comparative efficacy of seven selective media for isolating campylobacter jejuni.diarrheal stools from 263 patients were inoculated on seven selective media: butzler selective medium, blaser medium, skirrow blood agar, preston campylobacter selective medium, preston campylobacter blood-free medium, butzler virion medium, and modified preston medium (with amphotericin b [2 mg/liter]). a similar number of campylobacter jejuni strains were isolated from all the media studied; nevertheless, the presence of competing fecal flora (ff) made the detection of suspect colonies difficu ...19863760137
viable but nonculturable stage of campylobacter jejuni and its role in survival in the natural aquatic environment.conditions influencing the survival of campylobacter jejuni in the natural aquatic environment have been determined. release of campylobacter spp. into natural waters by animal hosts is postulated to play a key role in the maintenance of viability and transmission of the organism in the environment. laboratory flask microcosms containing filter-sterilized stream water were used to test c. jejuni for the ability to remain viable in simulated natural systems. the microcosms were compared with the ...19863767358
association with hela cells of campylobacter jejuni and campylobacter coli isolated from human feces.we developed a rapid in vitro test for determining the association of campylobacter jejuni and c. coli with hela cells. association was expressed as a weighted mean of the number of bacteria associated with one cell in an association index (ai). the reproducibility of the ai was checked by repeating the test six times, using four strains chosen at random. means and standard deviations of the means were 7.3 +/- 1.2, 6.8 +/- 0.9, 1.8 +/- 1.2, and 0.1 +/- 0.2. the experimental conditions for which ...19863770943
modified selective medium for isolation of campylobacter spp. from feces: comparison with preston medium, a blood-free medium, and a filtration system.our previously described (h. goossens, m. de boeck, and j. p. butzler, eur. j. clin. microbiol. 2:389-393, 1983) selective medium, consisting of cefoperazone (15 mg/liter), rifampin (10 mg/liter), colistin (10,000 iu/liter), and amphotericin b (2 mg/liter) (medium m1), for the isolation of campylobacter jejuni and campylobacter coli from stool specimens was modified as follows: cefoperazone (30 mg/liter), rifampin (10 mg/liter), and amphotericin b (2 mg/liter) (medium m2). a comparative study of ...19863771769
biotype and serogroup distribution of campylobacter isolates from children in nigeria.a total of 101 campylobacter isolates from nigerian children with or without gastroenteritis were biotyped and serogrouped by using the lior typing schemes (h. lior, j. clin. microbiol. 20:636-640, 1984; h. lior, d. l. woodward, j. a. edgar, l. j. laroche, and p. gill, j. clin. microbiol. 15:761-768, 1982). fifty-three (52.5%) of the isolates were campylobacter jejuni biotype i, 29 (28.7%) were c. jejuni biotype ii, 10 (9.9%) were campylobacter coli biotype i, and 9 (8.9%) were c. coli biotype i ...19863771772
rate of campylobacter spp. isolation in three regions of ontario, canada, from 1978 to 1985.isolation rates of campylobacter spp. were analyzed for an 8-year period, 1978 through 1985. three laboratories of the ontario ministry of health examined 146,842 human feces samples for bacterial pathogens, including campylobacter jejuni and campylobacter coli. campylobacter spp. were isolated from 5,580 specimens (3.8%), with monthly isolation rates ranging from 1.1 to 7.4%. the data showed a seasonal distribution of isolations, with peaks during the summer months (june to september). most inf ...19863771774
vaccination studies for the control of campylobacteriosis in jamaican cattle.following the first diagnosis of campylobacteriosis in jamaican cattle a field study was undertaken to determine the pathogenicity of campylobacter fetus subspecies venerealis jam (jamaican strain) and to evaluate the effectiveness of vaccination in controlling the disease. a total of 46 nonpregnant yearling heifers and four two-year-old bulls were used in two separate experiments. the results showed that c fetus subspecies venerealis jam readily colonised the reproductive tract of susceptible h ...19863776032
campylobacter fetus ss. jejuni: a cause of solitary cecal ulcer. 19863782763
lipopolysaccharide structures of campylobacter fetus are related to heat-stable determine whether lipopolysaccharide (lps) structures of campylobacter fetus are related to the three known heat-stable serogroups, proteinase k-treated whole cell lysates obtained from strains of each serogroup were electrophoresed in polyacrylamide gels. all strains had smooth-type lps with multiple high-molecular-weight repeating units. the profiles of serogroup a from c. fetus subsp. fetus and from c. fetus subsp. venerealis were identical, but they were different from those of c. fetus s ...19863510169
two-dimensional thin-layer chromatography for the specific detection of hippurate hydrolysis by microorganisms.glycine, one of the end products of hippurate hydrolysis by microorganisms, was detected by a rapid, specific technique utilizing two-dimensional thin-layer chromatography. a loopful of growth of each organism from its suitable agar medium was washed, suspended, and incubated with 0.1% sodium hippurate for 30 min at 37 degrees c. the supernatant of the incubated suspension from each organism was then dansylated, and the dansyl derivatives were separated by two-dimensional thin-layer chromatograp ...19863517036
enrichment for detection of campylobacter jejuni.gifu anaerobe-modified semisolid medium supplemented with vancomycin, polymyxin b, trimethoprim, and amphotericin b can serve as an efficient enrichment for the recovery of campylobacter jejuni under aerobic conditions. it is superior to the direct plating method in its recovery of very small numbers of c. jejuni. blood supplementation, agitation, and elevated temperatures are not required for this improved recovery.19863519661
antigenicity of campylobacter jejuni flagella.we studied the antigenicity of a wild-type flagellate and motile (f+m+) campylobacter jejuni strain (81116) and two daughter mutants, one flagellate and immotile (f+m-) and one aflagellate and immotile (f-m-). by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of acid-extracted surface proteins, a 63-kilodalton (kda) band identified from sheared flagella as the flagellar protein was present in the f+m+ and f+m- strains but not in the f-m- strain. no other differences in protein profile ...19863522430
abortion and perinatal sepsis associated with campylobacter infection.fetal loss or neonatal sepsis associated with campylobacter infection during pregnancy is infrequently recognized. as reported herein, one case of premature labor and neonatal sepsis due to campylobacter fetus subspecies fetus was treated successfully with ampicillin and gentamicin. only 19 similar cases have been cited in the literature. a review of these 19 cases reveals that the campylobacter species involved were probably c. fetus subspecies fetus in nine instances, campylobacter jejuni in n ...19863523697
pathogenesis of campylobacter spp. in athymic and euthymic germfree athymic (nu/nu) and euthymic (+/nu) germfree balb/c mice were orally challenged with pure cultures of campylobacter jejuni (human clinical fecal strains) and a human blood isolate of campylobacter fetus subsp. fetus. after a period of adaptation to the mouse intestinal tract, all three c. jejuni strains caused disease in gnotobiotic mice. mouse-adapted, weakly cytotoxic c. jejuni 45100 consistently induced disease symptoms (transient diarrhea, cecal shrinkage, and acute inflammatory change ...19863525411
direct immunofluorescence microscopy for rapid screening of campylobacter enteritis.diagnostic use of a direct fluorescent-antibody test for detection of campylobacter jejuni and c. coli in human fecal specimens (n = 497) was compared with detection by culturing (specificity, 99.7%; sensitivity, 40%). conjugates were prepared from immunoglobulin g antibody against 22 lior c. jejuni and c. coli reference strains (h. lior, d. l. woodward, j. a. edgar, l. j. laroche, and p. gill, j. clin. microbiol. 15:761-768, 1982). interestingly, the serotypes of cultures tested by the direct f ...19863533990
[right-sided endocarditis caused by campylobacter fetus]. 19863602699
plasmid-mediated tetracycline resistance in campylobacter jejuni: expression in escherichia coli and identification of homology with streptococcal class m determinant.plasmid pua466, a 45-kilobase transmissible tetracycline resistance plasmid from campylobacter jejuni was mapped with avai, avaii, bcli, hincii, psti, xhoi, and xbai. the resistance determinant was cloned and expressed in escherichia coli and was homologous with a class m determinant from streptococcus spp.19863005233
an epidemiological study of selected calf pathogens on holstein dairy farms in southwestern ontario.fecal samples from calves on 78 randomly selected holstein dairy farms in southwestern ontario were screened for salmonella, campylobacter jejuni/coli, enteropathogenic escherichia coli, rotavirus and coronavirus. based on the observed prevalence, 22% of farms had calves infected with salmonella, 13% with campylobacter jejuni/coli, 41% with enteropathogenic e. coli, 19% with rotavirus and 5% with coronavirus. these estimates can be modified, using a method developed by mullen and prost (1983) fo ...19863017528
cytotoxic and cytotonic factors produced by campylobacter jejuni, campylobacter coli, and campylobacter laridis.complete toxigenicity studies were performed on 341 strains of campylobacter spp., including 23 nonhuman isolates. toxin profiles based on both cytotonic and cytotoxic factors were determined after analyzing responses in vero, hela, cho and y-1 cells. suckling mouse assays were consistently negative for all culture filtrates tested. toxin-producing strains were frequently encountered among both the human and nonhuman strains of campylobacter jejuni, c. coli, and c. laridis investigated. strains ...19863018039
comparison between children treated at home and those requiring hospital admission for rotavirus and other enteric pathogens associated with acute diarrhea in melbourne, australia.the etiology of acute diarrhea in children less than 42 months of age attending one pediatric hospital in melbourne, australia, was studied during a 7-month period encompassing the winter of 1984. pathogens identified in 157 children treated as outpatients with mild disease were compared with those in 232 children hospitalized with severe disease. the pathogens (and frequencies among outpatients and inpatients, respectively) detected were rotaviruses (32.5 and 50.9%), enteric adenoviruses (8.9 a ...19863020082
genetic studies of kanamycin resistance in campylobacter jejuni.campylobacter jejuni 3h40 and 4b20 harbored 59-kilobase (kb) self-transmissible plasmids encoding resistance to kanamycin and tetracycline. although the two antibiotic resistances were more frequently inherited together, some transconjugants and ethidium bromide segregants which were resistant to only one of these antibiotics were recovered. the kanamycin-susceptible, tetracycline-resistant segregants carried plasmids 4 kb smaller than the 59-kb plasmids of their parents, whereas the kanamycin-r ...19863021049
mucus colonization as a determinant of pathogenicity in intestinal infection by campylobacter jejuni: a mouse cecal model.human isolates of the intestinal pathogen campylobacter jejuni have been shown to colonize mucus on the outer surface and deep within the intestinal crypts of gnotobiotic or germfree mice. the cecal crypts are preferentially colonized. a model of mucus colonization by c. jejuni in the mouse cecum has been developed, using antibiotic- and magnesium sulfate-treated specific-pathogen-free animals. these spiral-shaped bacteria colonize the mucus in a similar manner to the normal spiral-shaped microb ...19862935499
[campylobacter fetus ssp. fetus endocarditis]. 19862942861
[antibiotic resistance and distribution of plasmid-encoded beta- lactamases among agents of traveller's diarrhea].the polymicrobial aetiology of travellers's diarrhoea in 356 tourists travelling in thailand and burma was investigated. besides enterotoxigenic e. coli, salmonella sp. and campylobacter fetus ssp. jejuni were identified as the most important enteric pathogens. minimal inhibitory concentrations of several commonly used antibiotics were determined to reveal the percentage of enteric pathogens being resistant. 36.2% e. coli strains were found to be resistant to ampicillin and 14.3% of the campylob ...19862943094
influence of iron on growth, morphology, outer membrane protein composition, and synthesis of siderophores in campylobacter jejuni.three human isolates of campylobacter jejuni were grown in a biphasic culture medium with and without the addition of a synthetic chelator to induce iron limitation. cells grown in low-iron medium exhibited slower growth rates and altered cellular morphology. increased numbers of longer, more filamentous forms were seen in gram-stained smears. three proteins, with apparent mrs of 82,000, 76,000, and 74,000, were consistently present in the outer membrane of cells grown in low-iron medium. at lea ...19862944843
subdural empyema caused by campylobacter fetus ssp. fetus. 19862871115
identification and characterization of two campylobacter jejuni adhesins for cellular and mucous substrates.campylobacter jejuni is able to colonize the human intestinal mucosa and cause disease. for this reason, it was important to investigate mechanisms by which c. jejuni adheres to epithelial cells and intestinal mucus gel. all strains of c. jejuni used were able to adhere to int 407 epithelial cells and mucus, but high adherence to one substrate did not necessarily indicate comparable adherence to the other. the adherence of c. jejuni to cells was inhibited partially by treating the bacterial cell ...19862873103
substrate utilization by campylobacter jejuni and campylobacter attempt was made to elucidate in campylobacter spp. some of the physiologic characteristics that are reflected in the kinetics of co2 formation from four 14c-labeled substrates. campylobacter jejuni and c. coli were grown in a biphasic medium, and highly motile spiral cells were harvested at 12 h. of the media evaluated for use in the metabolic tests, minimal essential medium without glutamine, diluted with an equal volume of potassium sodium phosphate buffer (ph 7.2), provided the greatest s ...19862877615
monoclonal antibodies directed against the flagella of campylobacter jejuni: production, characterization and lack of effect on the colonization of infant mice.eight monoclonal antibodies have been derived from balb/c mice hyperimmunized with the purified flagella from campylobacter jejuni strain 81116. these monoclonal antibodies are directed against flagella as demonstrated by reaction in elisa against flagellate and aflagellate antigens, radio-immunoprecipitation and electro-immunoblotting techniques. some of the antibodies react with a 60k minor protein as well as the 62k flagella protein. this protein may be related to an antigen expressed on the ...19862422256
isolation and characterization of a common antigen in campylobacter jejuni and campylobacter coli.flagellin protein was isolated and purified from two serotype reference strains of campylobacter jejuni, pen 1 and pen 3. each preparation was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to consist of a diffuse band with a molecular mass of approximately 62 kilodaltons. antisera were prepared against flagellin from pen 1, and specific antibody was isolated by affinity chromatography with flagellin protein covalently bound to cyanogen bromide-activated sepharose. the high-a ...19862424932
location of epitopes on campylobacter jejuni flagella.flagella were isolated from strains of campylobacter jejuni belonging to different heat-labile serogroups and from a strain of campylobacter fetus, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the flagellin molecular weights (mr) were approximately 62,000. the flagellins were cleaved by hydrolysis with cyanogen bromide, and sodium dodecyl sulfate-urea peptide gel electrophoresis showed that the c. jejuni flagellins were structurally similar, and differed from c. fetu ...19862430943
lipopolysaccharide structures in enterobacteriaceae, pseudomonas aeruginosa, and vibrio cholerae are immunologically related to campylobacter determine whether lipopolysaccharide (lps) structures of campylobacter species are immunologically related to those of 11 other gram-negative organisms, we immunoblotted from polyacrylamide gels the lps of these strains with immune rabbit serum raised against six campylobacter jejuni strains and two campylobacter fetus strains. the lps studied were from salmonella minnesota wild type and ra to re mutants, salmonella typhi, escherichia coli, yersinia enterocolitica, vibrio cholerae, and pseudo ...19863079730
[two cases of campylobacter fetus meningitis]. 19863095453
[clinico-bacteriological study of diarrhea caused by campylobacter fetus subsp. fetus]. 19863095456
[morphological changes induced by ampicillin in campylobacter].morphologic changes in campylobacter fetus ssp. jejuni were evaluated as cultivated on thioglycollute broth, containing minimum of 0.1, 1.3 and 10 ampicillin minimal inhibitory concentration (mic). during the first four hours of incubation, no changes occurred in the presence of 0.1 mic; but with the rest of the ampicillin doses, even at the first hour, a proportion larger than 5% of filament-policurved forms was found, and it increased with the incubation time. the greatest number of altered ba ...19863671809
low incidence of campylobacter enteritis in northern a prospective survey carried out over 9 months in 1984 in the department of bacteriology, belfast city hospital, campylobacter jejuni was isolated from 24 out of 1200 faecal specimens (2%) from patients with acute diarrhoea. this isolation rate is much lower than that from other parts of mainland britain which report an isolation rate of between 8 and 15%. it is difficult to explain this large discrepancy but the limited availability of unpasteurized milk and the generally worse summer here ( ...19863794324
treatment of campylobacter gastroenteritis.twin boys suffered from recurrent diarrhoea due to campylobacter jejuni after entering a day nursery. stool sampling of all 17 children at the nursery revealed c. jejuni in 12. simultaneous treatment with antibiotics of all children with positive cultures successfully eradicated the infection.19873813645
demonstration of a flagellar antigen shared by a diverse group of spiral-shaped bacteria that colonize intestinal mucus.western blot analysis showed that there is little immunological cross-reactivity of the human pathogens campylobacter jejuni and campylobacter coli with campylobacter pyloridis, except for a very strong cross-reaction between the flagellins. this same antigenic cross-reaction was found with two isolates of gram-negative spiral microaerophilic bacteria that are known to colonize the intestinal mucosa of rodents, but not with the flagellins of a number of other motile bacteria. it is proposed that ...19873818099
pathogenesis of campylobacter fetus infections: serum resistance associated with high-molecular-weight surface proteins.campylobacter fetus subspecies fetus causes both systemic and diarrheal illnesses. we studied 38 strains of c. fetus isolated from 34 patients; underlying illness was present in eight (89%) of nine patients with only systemic isolates compared with three (20%) of 15 patients with only fecal isolates (p = .002). in a standardized assay of susceptibility to normal human serum, 27 (71%) strains were resistant, six (16%) had intermediate susceptibility, and five (13%) were serum sensitive. major pro ...19873819475
[septicemia caused by enteritis due to campylobacter fetus subspecies fetus].in a 55 year old man with renal insufficiency requiring hemodialysis after bilateral nephrectomy who had been hospitalized with febrile illness and enteritis, campylobacter fetus subspecies fetus could be isolated from the blood and feces. antibodies against the homologous strain (isolated from the blood) could be detected in the serum by means of the widal test and the indirect immunofluorescence test. culturing of campylobacter fetus subspecies fetus (especially from blood cultures) may be dif ...19873829936
[campylobacter fetus bursitis of the elbow]. 19873672019
survival of thermophilic campylobacters on fingertips and their elimination by washing and disinfection.a simple impression-plate technique has been used to investigate the survival of four thermophilic campylobacter strains applied to fingertips. campylobacters suspended in 0.1% peptone water and dried on the fingertips survived for different periods of time ranging from less than 1 to greater than or equal to 4 min. however, campylobacters suspended in chicken liquor or blood survived for much longer periods. the most resilient organism was campylobacter jejuni nctc 11392 which, when suspended i ...19873678391
laboratory and clinical evaluation of isolation media for campylobacter jejuni.six selective isolation media were evaluated for their ability to support the growth of campylobacter jejuni. colony counts of 70 isolated strains of c. jejuni and recovery studies on these strains in simulated positive feces samples demonstrated that bolton and hutchinson' charcoal, cefoperazone, deoxycholate agar and karmali's charcoal-based selective medium produced the highest recovery rates with the greatest suppression of other fecal flora. c. jejuni colonies were more easily recognized on ...19873429621
bovine abortion due to campylobacter fetus. 19873439950
the demonstration of the k-antigen of campylobacter fetus venerealis using a microtitre agglutination test.using specific antisera prepared against heat-treated and untreated cells in a microtitre system, 7 strains of campylobacter fetus venerealis were examined for the presence of k-antigen. the technique was effective for distinguishing between strains and it was found that 4 out of 7 isolates contained microcapsules, therefore being potentially suitable for vaccine production.19873444618
an examination of campylobacter fetus subsp. fetus by restriction endonuclease analysis and serology.restriction endonuclease analysis was used to examine 70 different strains of campylobacter fetus subsp. fetus, which were isolated from aborted sheep foetuses. the strains could be divided into seven types based on the dna fragment patterns obtained by electrophoresis after digestion with the enzyme bsteii. with one exception, digestion with the enzyme xhoi allowed the strains to be grouped identically to that for bsteii. antisera were made against formalized whole cells representative of each ...19872820112
gene transfer from escherichia coli to campylobacter species: development of shuttle vectors for genetic analysis of campylobacter jejuni.a shuttle cloning vector (pil550) has been constructed which can be mobilized from escherichia coli to campylobacter jejuni, campylobacter coli, and campylobacter fetus by complementation with the transfer functions of an incp plasmid in trans, with a frequency of 10(-4) transconjugants per donor. we also present evidence for a dna modification system in c. jejuni.19872822671
cloning and expression of a tetracycline resistance determinant from campylobacter jejuni in escherichia coli.the tetracycline resistance gene (tet) from the campylobacter jejuni plasmid pfkt1025 was cloned into both puc18 and pbr322 and was expressed when the chimeric plasmids were introduced into escherichia coli. the location of the tet determinant on the chimeric plasmids was determined by bal 31 deletion mapping within a 2.25-kilobase (kb) rsai-hincii fragment. a protein of approximately 70 kilodaltons was consistently produced by e. coli maxicells harboring the cloned tet determinant. a 500-base-p ...19872823694
in vitro activity of a-56268 (te-031), a new macrolide, compared with that of erythromycin and clindamycin against selected gram-positive and gram-negative organisms.the in vitro activity of a-56268 was determined and compared with that of erythromycin and clindamycin against a limited spectrum of 401 gram-positive and gram-negative organisms. a-56268 was quite active against erythromycin-susceptible staphylococcus aureus, neisseria gonorrhoeae, listeria monocytogenes, streptococcus pneumoniae, streptococcus pyogenes, and group b streptococci and was moderately active against campylobacter fetus subsp. fetus. a-56268 was consistently bactericidal only for s. ...19872952063
comparative in vitro activity of a-56268 (te-031) against gram-positive and gram-negative bacteria and chlamydia trachomatis.the in vitro activity of a-56268 (te-031) was determined and compared with that of 13 antibiotics against 401 gram-positive and gram-negative bacteria and 11 strains of chlamydia trachomatis. a-56268 was very active against methicillin-susceptible staphylococcus aureus and neisseria gonorrhoeae, and was among the most active of the agents tested against listeria monocytogenes, streptococci and chlamydia trachomatis. it was moderately active against haemophilus spp., vibrio spp., campylobacter je ...19872954818
characterization and expression of a cloned tetracycline resistance determinant from campylobacter jejuni plasmid pua466.a tetracycline resistance (tcr) determinant previously cloned from the campylobacter jejuni plasmid pua466 (d. e. taylor, j. bacteriol. 165:1037-1039, 1986) was localized by restriction endonuclease mapping, subcloning, and tn1000 insertion mutagenesis to a 2-kilobase region consisting of 1.8- and 0.2-kilobase hincii fragments. tcr encoded by the cloned fragment (puoa1) was expressed constitutively in escherichia coli. a protein with an apparent molecular weight of 68,000 encoded by puoa1 was pr ...19873036765
antimicrobial resistance of campylobacter jejuni and campylobacter coli with special reference to plasmid profiles of japanese clinical isolates.a total of 111 clinical isolates of campylobacter jejuni and 10 clinical isolates of campylobacter coli were characterized by their susceptibility to nine antimicrobial agents and by their plasmid profiles on agarose gel electrophoresis. all of the c. jejuni isolates were susceptible to chloramphenicol, ciprofloxacin, erythromycin, kanamycin, and nalidixic acid, but 55% were tetracycline resistant. in the 10 c. coli isolates, a high prevalence of multiple-antibiotic resistance was noted. plasmid ...19873038005
two-year survey of etiologic agents of diarrheal disease at san lazaro hospital, manila, republic of the philippines.the prevalence of bacterial pathogens and rotavirus in 2,908 patients with diarrhea who were admitted to san lazaro hospital in manila in 1983 and 1984 was determined. one or more enteric pathogens were isolated or detected in samples from 1,698 (58.4%) patients. isolation rates for the various enteropathogens were as follows: rotavirus, 30.6%; shigella spp., 11.6%; salmonella spp., 9.2%; enterotoxigenic escherichia coli (1983 only), 7.8%; vibrio cholerae biotype eltor, 3.8%; non-o1 v. cholerae, ...19873038946
isolation of campylobacter spp. from pigeon feces by a combined enrichment-filtration technique.a technique combining enrichment in preston enrichment broth and direct filtration onto chocolate agar was used to isolate campylobacter species from pigeon feces. campylobacter jejuni was isolated from 106 of 200 samples tested; 105 strains were isolated by enrichment-filtration, and 84 strains were isolated by direct plating. most of the strains grew after 48 h at 37 degrees c.19873606114
atypical campylobacters associated with gastroenteritis.nine strains of campylobacter species other than campylobacter jejuni, campylobacter coli, and campylobacter laridis were isolated from patients with acute diarrhea. all nine strains showed preferred growth at 37 degrees c under microaerophilic conditions. conventional microbiological tests and dna-dna dot blotting were used to identify these strains. three of the nine campylobacter strains hydrolyzed hippurate, reduced nitrate, produced catalase, were resistant to cephalothin, and were shown to ...19873611317
kinetics of anti-campylobacter jejuni monomeric and polymeric immunoglobulin a1 and a2 responses in serum during acute enteritis.the intensity and kinetics of the serum polymeric and monomeric immunoglobulin a1 (iga1) and iga2 antibody responses to campylobacter jejuni were analyzed. a rapid and marked serum iga antibody response involving both the monomeric and polymeric components of iga was observed after c. jejuni infections. iga antibodies reached a peak of activity in serum during week 2 after the first symptoms of enteritis, about 10 days before the peak of igg activity. polymeric iga accounted for most of the anti ...19873611318
utilization of monoclonal antibodies to evaluate the involvement of campylobacter jejuni in proliferative ileitis in syrian hamsters (mesocricetis auratus).the role of campylobacter jejuni in the pathogenesis of proliferative ileitis in syrian hamsters was evaluated with monoclonal antibodies of different specificities. monoclonal antibodies were produced with two different specificities: one for all members of the genus campylobacter tested (antibody 8322-2e6) and one for c. jejuni and campylobacter coli (antibodies 841-2a11, 841-4c6, and 841-5b1). heal sections from healthy hamsters, from hamsters with naturally occurring proliferative ileitis, a ...19873623701
evaluation of gamma radiation levels for reducing pathogenic bacteria and fungi in animal sewage and laboratory effluents.sewage samples collected from animal wastes and from effluents at an animal disease laboratory were inoculated with known numbers of pathogenic organisms and subjected to various doses of gamma radiation from a 60co source. surviving test organisms were quantitatively determined by selective and enrichment techniques. the experiment was modeled as a quantal assay in which probit analysis was applied to obtain d10 values. the d10 value represents the irradiating dose required to reduce the popula ...19873651881
production of a unique cytotoxin by campylobacter jejuni.campylobacter jejuni is an important diarrheal pathogen worldwide; the mechanisms by which it causes disease remain unclear. because of its association with inflammatory diarrhea, we postulated that c. jejuni might produce a cytotoxin similar to that produced by shigella sp., enterohemorrhagic escherichia coli o157, or clostridium difficile. filtrates of 12 polymyxin-treated isolates of c. jejuni were placed on hela cells (sensitive to shiga toxin cytotoxicity) and chinese hamster ovary (cho) ce ...19873653987
naturally occurring auxotrophs of campylobacter jejuni and campylobacter coli.the nutritional requirements for 439 campylobacter jejuni isolates and 46 campylobacter coli isolates were determined by using a previously described chemically defined medium, campylobacter defined medium. with this medium, 45% of both human and nonhuman c. jejuni isolates demonstrated auxotrophic requirements. none of the 46 c. coli isolates studied demonstrated requirements for amino acids on campylobacter defined medium. the most common auxotrophic requirement among c. jejuni isolates was fo ...19873654939
prevalence and characterization of hippurate-negative campylobacter jejuni in king county, washington.a total of 593 strains of thermophilic campylobacter species were isolated either from humans with diarrhea or from poultry in king county, washington. of these strains, 98 (52 hippurate-positive strains and all 46 of the hippurate-negative strains) were selected for further phenotypic characterization and genetic classification. hippurate hydrolysis, the test typically used to differentiate campylobacter jejuni and c. coli, did not always correlate with the genetic classification. all hippurate ...19873654945
rapid and improved gas-liquid chromatography technique for detection of hippurate hydrolysis by campylobacter jejuni and campylobacter coli.a gas-liquid chromatographic method which requires no chloroform extraction of the split products has been investigated for the detection of hippurate hydrolysis by campylobacter spp. this technique gave better reproducibility than other tests also used in this study and allows the routine use of the gas-liquid chromatographic method for identification of campylobacter isolates.19873654950
problems in identification of campylobacter jejuni associated with acquisition of resistance to nalidixic a patient with campylobacteriosis, we observed development of resistance to nalidixic acid and norfloxacin under adequate treatment with the latter antibiotic. isolates before and after treatment differed in their total protein profile but were otherwise identical with respect to enzymatic activity, serotype, crude membrane protein profiles, and other phenotypic characteristics.19873654954
susceptibility of campylobacter jejuni and yersinia enterocolitica to uv radiation.two enteric pathogens, campylobacter jejuni and yersinia enterocolitica serogroup o:3, together with escherichia coli, were investigated for susceptibility to uv radiation at 254 nm. the uv dose required for a 3-log reduction (99.9% inactivation) of c. jejuni, y. enterocolitica, and e. coli was 1.8, 2.7, and 5.0 mws/cm2, respectively. using e. coli as the basis for comparison, it appears that c. jejuni and y. enterocolitica serogroup o:3 are more sensitive to uv than many of the pathogens associ ...19873551844
variations in the virulence, for pregnant guinea pigs, of campylobacters isolated from man.pregnant guinea pigs were used to compare the virulence of four human isolates of campylobacter fetus ss. fetus and four of c. jejuni on the basis of their ability to cause abortion and bacteraemia. of the four strains of c. fetus ss. fetus two produced abortion readily after intramuscular injection. the four c. jejuni isolates were, however, of comparatively low virulence and no differences between them were demonstrated. some of the isolates differed in their ability to survive in vitro in hum ...19873560189
[infections caused by campylobacter fetus]. 19873561577
intestinal mucus gel and secretory antibody are barriers to campylobacter jejuni adherence to int 407 in vitro mucus assay was developed to study the role of mucus gel and secretory immunoglobulin a (siga) in preventing attachment of campylobacter jejuni to int 407 cells. an overlay of rabbit small intestinal mucus was found to impede the attachment of c. jejuni to a monolayer of int 407 cells. mucus from rabbits previously colonized with c. jejuni was found to completely inhibit bacterial adherence to the underlying cells. anti-campylobacter siga was readily detected in mucus samples from pr ...19873570473
campylobacter-associated appendicitis: prevalence and clinicopathologic determine the prevalence of campylobacter fetus subspecies jejuni-associated appendicitis, we studied, retrospectively, by means of immunohistochemistry, the appendectomy specimens of 116 consecutive patients, operated upon because of suspected acute appendicitis. we found immunohistochemical evidence of campylobacter fetus subspecies jejuni infection in three patients. these findings were confirmed by electron microscopy. based upon these three cases and five additional appendectomy specimen ...19873574983
seasonal occurrence of campylobacter spp. in surface waters and their correlation with standard indicator bacteria.campylobacter jejuni, campylobacter coli, and a campylobacter-like organism were isolated from a number of natural water sources in central washington, including ponds, lakes, and small mountain streams at elevations ranging from 1,460 to 5,400 feet (ca. 445 to 1,646 m) above sea level. at the two sites where extensive sampling was done, the bacteria were recovered throughout the year. generally, the recovery rates were highest in the fall and winter months and lowest during the spring and summe ...19873579268
campylobacter jejuni in dairy cows and raw milk.twelve herds of dairy cows were examined by rectal swabbing for the presence of campylobacter jejuni. ten herds were positive with the incidence of colonized animals ranging from 10 to 72% of those tested. with the exception of the two negative herds where mains water only was consumed, all animals drank from rivers or streams when grazing. there was no relationship between total and coliform counts and the presence of c. jejuni in raw milk. however, milk from one farm that consistently gave pos ...19873595744
acid and alkaline campylobacter extracts in the demonstration of antibody response in hyperimmunized rabbits.acid and alkaline extracts from several campylobacter strains were used as antigens in enzyme immunoassay (eia). antibodies were detected in rabbits immunized with formalin-treated, boiled, or autoclaved whole bacteria of different campylobacter subspecies. for the preparation of the extracts, three campylobacter fetus subsp. venerealis strains, three campylobacter fetus subsp. fetus strains, four campylobacter coli, and five campylobacter jejuni strains were used. the acid extracts of campyloba ...19873296648
multiple enteric pathogens in a patient with diarrhoea.a woman who recently arrived in canada from india developed a febrile, diarrhoeal illness. stool culture revealed the presence of salmonella typhi, campylobacter fetus subspecies jejuni and shigella flexneri. concurrent infection with these three pathogens does not appear to have been previously reported. the possibility of multiple pathogens should be considered in travellers who fail to respond to treatment of a single organism.19873299934
structural and antigenic properties of lipopolysaccharides from serotype reference strains of campylobacter investigate the molecular basis for heat-stable antigenic diversity in campylobacter jejuni, lipopolysaccharides (lpss) from serotype reference strains and serotyped isolates were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis coupled with silver staining and immunoblotting. by silver staining, only low-mr components, consisting of one major band and as many as three minor bands ranging in mr from 4,500 to 5,000, were detected. however, by immunoblotting with homologous ...19873301678
expression of campylobacter jejuni invasiveness in cell cultures coinfected with other bacteria.enteroinvasive salmonella, shigella, and escherichia coli strains were found to exert an effect which rendered campylobacter jejuni capable of intracellular localization in epithelial cells in vitro. when monolayers of hep-2 or a-549 cells were challenged with pure cultures of c. jejuni or campylobacter coli, none of the eight strains tested invaded the cells. in contrast, four of these strains were able to localize intracellularly when the cells were challenged with a mixture of campylobacters ...19873312015
meningitis due to campylobacter fetus intestinalis in a kidney transplant recipient. a case report.a 47-year-old man developed campylobacter fetus intestinalis meningitis 3 years after cadaver renal transplantation. the infection was successfully controlled with chloramphenicol followed by erythromycin. there were no relapses during the past 5 years and the allograft function remains normal. the available evidence suggests that the campylobacter sepsis was associated with nutritional therapy, the patient received in a mexican clinic. the present case is reported because of its clinical import ...19873324765
a comparative study of the growth of campylobacter fetus strains in liquid media.the growth of c. fetus fetus, c. fetus venerealis and c. fetus venerealis biotype intermedius was examined in 10 liquid media. from the data obtained, a 10% inoculum size and an oxygen level of 6% seemed imperative for consistent growth, especially for the c. fetus venerealis strain. a lowered redox potential obtained by the addition of 0, 1% cysteine-hc1 to the media was stimulatory. the medium which yielded the best growth was the one described by dennis & jones (1959). the fastidious c. fetus ...19873328136
cytotoxic and enterotoxic activities of campylobacter jejuni are not specified by tetracycline resistance plasmids pmak175 and pua466.the 45-kilobase tetracycline resistance plasmids pmak175 and pua466 from campylobacter jejuni were examined using curing and mating experiments. however, these plasmids encoded neither cytotoxin production, as determined in vero cells, nor enterotoxin activity, as determined in chinese hamster ovary cells.19873793867
evaluation of four enrichment media for isolation of campylobacter jejuni.diarrheal stool specimens were inoculated into the following media: alkaline peptone water (apw), bruce-zochowsky medium (bz), campylobacter enrichment broth (ceb), campy-thio broth (ct), and skirrow blood-agar (sk) plate. all media were incubated at 42 degrees c in microaerophilic conditions for 24 h. afterwards, a new sk plate was inoculated from every liquid medium. campylobacter jejuni was isolated from 43 of the 259 specimens when ct was used, from 45 when apw was used, from 46 when bz was ...19873793873
mucosal and systemic immunity to campylobacter jejuni in rabbits after gastric inoculation.the mucosal and systemic immune responses to campylobacter jejuni were studied in rabbits receiving gastric inoculation with live organisms. a lavage procedure was used to facilitate repeated monitoring of the intestinal immune response to c. jejuni. immunity to c. jejuni was determined by secondary challenge by using the removable intestinal tie adult rabbit diarrhea (ritard) model and monitoring for resistance to colonization and bacteremia. oral-gastric inoculation of normal rabbits produced ...19883335413
the extent of surface contamination of retailed chickens with campylobacter jejuni serogroups.eighty-two chickens purchased at 11 retailers (supplied by 12 wholesalers) in the south of england were cultured for campylobacter jejuni by a method involving total immersion. the organism was isolated from 22 (48%) of 46 fresh birds, 12 of 12 uneviscerated (new york dressed) birds, but only 1 of 24 frozen birds. viable counts of up to 1.5 x 10(6)/chicken were obtained from fresh birds and 2.4 x 10(7)/chicken from uneviscerated birds. surface swabbing of breasts, thighs, wings and vents of fres ...19883338503
experimental colonization of broiler chicks with campylobacter jejuni.minimal colonization inocula for two broiler strains of campylobacter jejuni were determined in broiler chicks aged 2-3 days and 2 weeks. individually housed chicks were exposed to a single oral or cloacal challenge. diarrhoeal symptoms were absent in all 380 chicks included in the study. chick susceptibility to the two c. jejuni strains varied. colonization was effected by less than 10(2)-10(4) colony forming units (c.f.u.) via cloacal challenge and 10(4)-10(6) c.f.u. via the oral route. coloni ...19883338504
enteropathogens associated with acute infantile diarrhoea in cape town.the results of a year-long controlled study on the causation of acute infectious diarrhoea in infants are reported. campylobacter fetus subspecies jejuni, rotavirus and certain enteropathogenic escherichia coli (epec) types were identified as the most common enteropathogens. shigella, salmonella group b and yersinia enterocolitica were significant but less common pathogens. campylobacter, rotavirus, the significant epec types and shigella infections were more frequent in the warm summer and autu ...19883340928
characterization of an unclassified microaerophilic bacterium associated with gastroenteritis.four isolates of an unclassified microaerophilic bacterium resembling campylobacter species were characterized by growth requirements, microscopic examination, biochemical characteristics, antimicrobial susceptibility tests, and protein profile analysis. the unclassified isolates were differentiated from campylobacter jejuni, campylobacter coli, campylobacter fetus subsp. fetus, campylobacter laridis, campylobacter pylori, and an ovine isolate. the bacterium was fusiform shaped with a corrugated ...19883343302
evaluation of the campyslide agglutination test for confirmatory identification of selected campylobacter species.the utility of a rapid latex slide agglutination test (campyslide; bbl microbiology systems, cockeysville, md.) in detecting selected campylobacter spp. was evaluated and compared with that of conventional identification methods. isolated colonies suggestive of campylobacter spp. were tested directly from primary selective media after incubation at 42 degrees c under microaerophilic conditions. stock cultures of campylobacter jejuni (n = 27) and c. coli (n = 3) were correctly confirmed to the ge ...19883343315
infertility in cattle in south-west scotland caused by an 'intermediate' strain of campylobacter fetus subspecies fetus (formerly campylobacter fetus intestinalis). 19883345413
concurrent bovine virus diarrhoea virus and campylobacter fetus infection in an aborted bovine fetus. 19883354166
characterization of freshly isolated campylobacter coli strains and suitability of selective media for their growth.typical and atypical campylobacter strains were isolated from the colons of cattle and swine by techniques that enabled the selective pressures of antibiotics to be avoided. some cephalothin-susceptible strains and a strain with an indeterminate hippurate reaction were classified as campylobacter coli by dna homology testing. tetracycline-resistant isolates were obtained from animals with no recorded exposure to antibiotics. a selection of 12 c. coli and 6 c. jejuni strains was used to determine ...19883356790
campylobacter jejuni antibodies in nigerian children.titers of complement-fixing (cf) antibody to campylobacter jejuni were demonstrated in 87 (36.7%) of 237 infants 6 to 15 months old in jos, nigeria. of the total number of children examined, 81 had acute diarrhea and 27 of them (33.3%) were found to have cf antibodies in their serum. the remaining 156 children were asymptomatic, and 60 (38.4%) of them had cf antibodies. in the diarrheal group, 27 of 75 children 6 to 8 months old were cf antibody positive. there was no significant difference in t ...19883356797
purification and characterization of a family of high molecular weight surface-array proteins from campylobacter fetus.a variety of gram-negative and gram-positive bacteria possess crystalline surface layers, although little is known of their function. we previously have shown that the high molecular weight surface-array proteins of campylobacter fetus are important in both the pathogenicity and antigenicity of this organism. for biochemical and immunological characterization, we purified high molecular weight (100,000, 127,000, 149,000) surface-array proteins from three c. fetus strains using sequential gel fil ...19883360785
pathogenesis of campylobacter fetus infections. failure of encapsulated campylobacter fetus to bind c3b explains serum and phagocytosis resistance.campylobacter fetus ssp. fetus strains causing systemic infections in humans are highly resistant to normal and immune serum, which is due to the presence of high molecular weight (100,000, 127,000, or 149,000) surface (s-layer) proteins. using serum-resistant parental strains (82-40 lp and 23d) containing the 100,000-mol wt protein and serum-sensitive mutants (82-40 hp and 23b) differing only in that they lack the 100,000-mol wt protein capsule, we examined complement binding and activation, an ...19883366901
cryopreservation studies of strains of campylobacter fetus ss. fetus, one of campylobacter fetus ss. venerealis, and one of campylobacter jejuni were preserved using a variety of cryopreservation methods. organisms were frozen to -150 degrees c in a liquid nitrogen refrigerator, in the freezer compartment of a refrigerator (-20 degrees c), and in a mechanical freezer (-65 degrees c). in the latter two cases, viabilities of the organisms were compared after being frozen in brucella albimi broth and 10% glycerol. viabi ...19883371059
chemotactic behavior of campylobacter jejuni.the chemotactic behavior of campylobacter jejuni was determined in the presence of different amino acids, carbohydrates, organic acids, and preparations and constituents of mucin and bile. l-fucose was the only carbohydrate and l-aspartate, l-cysteine, l-glutamate, and l-serine were the only amino acids producing a chemotactic (positive) response. several salts of organic acids, including pyruvate, succinate, fumarate, citrate, malate, and alpha-ketoglutarate, were also chemoattractants, as were ...19883372020
the immune system response to campylobacter infection.campylobacter may be one of the most common causes of bacterial gastroenteritis (ge) in children. it has recently been suggested that it is one of the bacterial pathogens most likely to infect immune-compromised children, and it may facilitate colonization of enteric pathogens. the immune system response was studied in 12 children with campylobacter fetus subspecies jejuni (cbj) infections. serum concentrations of iga, igm, and igg were analyzed using a beckman auto-analyzer. sera specific ab to ...19883374406
small-scale dna preparation for rapid genetic identification of campylobacter species without radioisotope.a simplified and rapid genetic identification method for campylobacter species without radioisotope was established. three different amounts of dna (200, 50, and 12.5 ng) extracted from each type strain of campylobacter species with standard marmur's procedure were spotted on a nitrocellulose filter. dna obtained from one ml bacterial suspension at a concentration of mcfarland standard turbidity no. 1 of campylobacter fetus, c. jejuni, c. coli, and c. pylori isolates were sufficiently labeled wi ...19883374408
vaccination of pregnant guinea pigs with campylobacter fetus: effects of antigen dose, campylobacter strain, and adjuvant type.for the purpose of developing and improving vaccines to protect cattle against bovine genital campylobacteriosis, the interaction of vaccine dose, strain(s) of campylobacter fetus, and type of adjuvant was studied in the pregnant guinea pig model. five strains of c fetus were used in the preparation of 20 monovalent vaccines containing various doses of bacteria (0.1 mg to 20 mg, dry weight) in freund incomplete adjuvant, freund complete adjuvant, or aluminum hydroxide gel adjuvant. vaccinal prot ...19883377303
improved blood free selective medium for isolating campylobacter jejuni from faecal specimens. 19883385002
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