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heat injury and repair in campylobacter jejuni.a procedure for detecting and quantitating heat injury in campylobacter jejuni was developed. washed cells of c. jejuni a7455 were heated in potassium phosphate buffer (0.1 m, ph 7.3) at 46 degrees c. samples were plated on brucella agar supplemented with na2s2o3, feso4 x 7h2o, and sodium pyruvate and on a medium containing brilliant green, bile, na2s2o3, feso4 x 7h2o, and sodium pyruvate. colonies were counted after 5 days of incubation at 37 degrees c in an atmosphere containing 5% o2, 10% co2 ...19846497368
use of an ammonia electrode to study bacterial deamination of amino acids with special reference to d-asparagine breakdown by campylobacters.a method using an ammonia electrode is being developed for investigating the deamination of amino acids and amides by bacteria. application of this method to campylobacter jejuni and c. coli has led to the demonstration of d-asparaginase activity in some strains. this has allowed the subdivision of both species into d-asparaginase-positive and -negative biotypes. even though the method is in the developmental stage, it was found to be generally reproducible and easy to perform. areas for further ...19846501873
epidemiological investigations on campylobacter jejuni in households with a primary infection.fifty-four rotterdam patients in which a primary infection with campylobacter jejuni had been detected (index patients) were compared with 54 control subjects with regard to the consumption and preparation of foods 7 days before onset of illness and the keeping of pet animals. significantly more index patients than controls had eaten chicken meat (47 v. 29; p = 0.0002), particularly at barbecues (14 v. 2; p = 0.0015). marginally more index patients had eaten pork (47 v. 39; p = 0.048) or inadequ ...19846501878
a search for the source of campylobacter jejuni in milk.samples of milk from 1501 cows with mastitis were negative for campylobacter jejuni. the faeces of 74 healthy friesian cows were screened for c. jejuni: 13% of the samples were positive during the summer when the cows were on pasture, and 51% were positive in the winter when the cows were housed. positive samples contained on average 1 x 10(4) campylobacters per g faeces. it is concluded that faecal contamination rather than udder infection is the means by which campylobacters enter milk and the ...19846501879
quantitative evaluation of a transport-enrichment medium for campylobacter fetus.the enrichment feature of a selective serum-based transport medium for campylobacter fetus was quantitatively examined. preputial samples from artificial insemination bulls were spiked with known numbers of c fetus strains and inoculated into transport-enrichment medium (tem). the survival and multiplication of these strains in tem under different incubation periods and temperatures were assessed by plate counts. mean enrichment values of 3.72 log and 4.42 log were observed after incubation at 3 ...19846506432
comparison of antimicrobial susceptibility patterns of campylobacter jejuni and campylobacter coli.to determine whether employing antibiograms is useful to separate campylobacter jejuni and campylobacter coli, we determined the mics of 12 antibiotics for 104 human clinical strains and 74 swine strains. of 74 swine strains, 5 (7%) were hippurate positive, as were 93 (89%) of 104 human strains. the 12 antimicrobial agents tested were ampicillin, amoxicillin, clindamycin, chloramphenicol, erythromycin, furazolidone, norfloxacin, nalidixic acid, rosoxacin, rosaramicin, tetracycline, and sch 32063 ...19846508265
improved biotyping schemes for campylobacter jejuni and campylobacter coli.campylobacter jejuni (20 strains) and campylobacter coli (12 strains) were assigned to four biovars for each species based on phenotypic tests that were easy to perform and interpret. the resulting biotyping schemes offer a greater degree of distinction among c. jejuni and c. coli strains than any of the other biotyping schemes previously described for these organisms.19846511880
serotyping and serology studies of campylobacteriosis associated with consumption of raw milk.a community outbreak of 15 cases of gastroenteritis was traced to consumption of unpasteurized milk produced at one commercial dairy. using two different testing schemes, we found that a campylobacter jejuni isolate from an ill patient and an isolate from a sick cow were the same serotype. bacteriological studies suggested that a single epidemic strain of campylobacter jejuni caused this outbreak.19846511884
antibacterial activities of nitrothiazole against campylobacter jejuni and campylobacter coli.niridazole (ambilhar) and three other newly synthesized nitrothiazole derivatives were highly active against 19 microaerophilic campylobacters (minimum concentration required to inhibit 50% of strains [mic50], 0.0075 to 0.015 mg/liter). there were, however, considerable differences in the susceptibility among strains tested, and one nitrothiazole derivative was rather inactive (mic50, 2 mg/liter). nitroimidazole derivatives, such as metronidazole and tinidazole, were less active (mic50, 2 and 4 ...19846517542
composition of the antigenic material removed from campylobacter jejuni by heat.the antigenic material removed form campylobacter jejuni by the boiling of whole cells in saline was examined biochemically. analyses showed that the extracted material contained 3 micrograms of protein per ml per mg of wet cells and ca. 2.6 micrograms of carbohydrate per ml per mg of wet cells. further extraction of the material with chloroform-methanol produced about 0.5 microgram of water-insoluble residue per ml per mg of wet cells, suggesting the presence of lipid as well. additional analys ...19846520219
levels of some reproductive diseases in the dairy cattle of colombia.of 4,144 serum samples collected from cows on 113 farms from eight areas of colombia 3.3% had positive and 8.8% inconclusive titres to brucella abortus, 21.7, 6.3, 1.6, 0.6 and 0.7% of cows had positive titres to leptospira serovars hardjo, pomona, canicola, icterohaemorrhagiae and grippotyphosa respectively. questionnaires completed on 110 farms revealed that 6, 2.5 and 4.6% of cows had had metritis, aborted or retained their placentas respectively in the previous 12 months. trichomonas foetus ...19846523582
[diarrhea caused by campylobacter fetus jejuni and other infective agents in children of the rural area of puriscal, costa rica].between september 1979 and september 1981 a field study was conducted on the etiology of diarrheal disease in the area of puriscal, costa rica. the presence of enteric pathogens was investigated in the stools of 267 diarrheic children and 190 healthy controls. both groups belong to yearly cohorts recruited at birth as part of a longitudinal multidisciplinary study of mothers and children. campylobacter fetus jejuni was identified as the only pathogen in the stools of 24 diarrheic children (9%) a ...19846535179
[campylobacter fetus subsp. jejuni: etiological, epidemiological and preventive aspects].the authors, after a brief introduction, examine on the basis of the most recent literature, the taxonomy and the microbiological features of campylobacter fetus subsp. jejuni, giving a detailed description of the chemical composition, of the biochemical, physiological and nutritional characters, of the antigenic composition and of the cultural methods. later on, they state the problems arising from the epidemiology of the infections caused by this germ either in man or in animals, examining the ...19846536547
growth and survival characteristics of campylobacter jejuni in liquid egg.growth and survival of four campylobacter jejuni strains in yolk, in liquid whole egg and in white during aerobic storage at 37, 20 and 4 degrees c was followed. in 48 h at 37 degrees c the cell counts of c. jejuni increased by about 3 log10 units in yolk and 1.60-3.35(10) log units in liquid whole egg. the growth of c. jejuni was slightly better in yolk than in liquid whole egg. at 20 degrees c during 48 h the cell counts decreased by about 0.5-1.5 log10 units in yolk and in liquid whole egg. a ...19846537959
[rotavirus and campylobacter fetus jejuni associated with an outbreak of diarrhea in calves].rotaviruses and campylobacter fetus jejuni are ubiquitous agents of diarrheal disease in animals and humans. under natural conditions they do not seem to cross inter-species barriers; a zoonosis has not been documented for man. however, animal rotaviruses might contribute to the emergence of new reassortment strains in view of their segmented genome, and thus, produce new antigenic variants. on the contrary, campylobacter fetus jejuni produces a true zoonosis. man acquires bacilli by ingesting w ...19846545623
the effect of storage in liquid nitrogen on the recovery of human dental plaque bacteria.centrifuged deposits of various individual bacteria from type-culture collections and dental plaque samples were immersed in liquid nitrogen for periods of up to 2 years. all the organisms were recovered after storage in liquid nitrogen but fusobacterium nucleatum (atcc 10953) and campylobacter fetus (nctc 10354) consistently showed a reduction in the number of colony-forming units after freezing. the main effect on bacterial proportions in plaque samples was a reduction in aerobic gram-negative ...19846596040
the microbial evaluation of acute cellulitis.twenty consecutive patients with acute cellulitis were studied by retrospective chart review. patients with suffusion and edema, with or without vesicles, were included. patients with breaks in the skin, with focal suppurations or with concurrent antimicrobial therapy were excluded. in nineteen of the twenty patients, aspirates (95 percent) failed to grow organisms. pasteurella multocida was grown from subcutaneous aspirate and blood cultures of a single patient; he was immunocompromised by wald ...19836602690
hla-b27-negative arthritis related to campylobacter jejuni enteritis in three children and two adults.five out of 37 patients with proven campylobacter jejuni enteritis developed arthritis. two adult patients presented with classical reiter's syndrome. one of the three children had reactive arthritis, and clinical suspicion of septic arthritis could not be confirmed in two. the acute synovitis subsided usually without treatment in all patients within 3-7 days, while arthralgia persisted longer in 4 patients. hla-b27 was not present in the 5 patients with arthritis, but was found in 4 others. in ...19836605028
a case of a double infection with salmonella typhi and campylobacter fetus subspecies jejuni.a case of a double infection with salmonella typhi and campylobacter subspecies jejuni proven by isolation and serology is reported. the patient was effectively treated with erythromycin and chloramphenicol.19836612785
campylobacter-induced toxic megacolon.a case of a compromised host with myeloproliferative syndrome who presented with severe, relentless but nonbloody diarrhea, fever, severe colitis, and toxic megacolon is presented. blood cultures grew out campylobacter fetus ssp. fetus. specific treatment with erythromycin reversed the grave clinical picture to normal in a 5-day period.19836613968
[campylobacter fetus bacteremia]. 19836618327
identification and characterization of campylobacter jejuni outer membrane proteins.outer membrane proteins from isolates of campylobacter jejuni were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. sarcosinate-insoluble membrane preparations were outer membrane enriched based on increased ketodeoxyoctonate concentrations, the presence of surface-exposed 125i-labeled proteins that were hydrophobic, and similarity to membrane vesicle (bleb) sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles. most isolates contained a single major band with ...19836618667
extended scheme for serotyping campylobacter jejuni: results obtained in israel from 1980 to 1981.the serotyping scheme for campylobacter jejuni previously developed in the national center for campylobacter, jerusalem, was extended by the use of 20 new sera and modified by the absorption of the sera, when necessary, with homologous boiled cultures or heterologous live cultures. the extended scheme is based on slide agglutination of live suspensions and is performed in two stages: pretesting with four pooled sera and final testing with monovalent sera. so far, 34 serotypes have been recognize ...19836619282
electron microscopy of the coccoid form of campylobacter jejuni.confluent cultures of campylobacter jejuni incubated for 24 and 48 h each were examined by electron microscopy. although the 24-h-old cells exhibited typical curved morphology, the 48-h-old cells showed rounded morphology with a loss of cell integrity. this appeared to be an autolytic process that occurred very rapidly after the culture became mature. these results confirm previous evidence that the coccoid form of this organism is a degenerate state.19836619291
[occurrence of campylobacter fetus subsp. jejuni in mice and guinea pigs from experimental animal establishments]. 19836624307
inhibitory and lethal activities of rosaramicin, erythromycin, and clindamycin against campylobacter fetus subsp jejuni and intestinalis.in a comparative study of the inhibitory and lethal effects of rosaramicin, erythromycin, and clindamycin on strains of campylobacter fetus subsp jejuni and c intestinalis, c jejuni was more readily killed by rosaramicin and clindamycin than was c intestinalis. erythromycin exhibited an equally lethal effect against both subspecies. however, it was the least active of the macrolides tested against both c jejuni and c intestinalis.19836625312
[erythema nodosum and campylobacter fetus infection]. 19836625463
isolation of campylobacter jejuni from raw milk.campylobacter jejuni was isolated from raw milk by a method that can routinely detect less than or equal to 1 organism per ml. this procedure was used in a survey of 195 separate farms and showed a 1.5% incidence of c. jejuni in milk from bulk tanks.19836625570
enhanced isolation of campylobacter jejuni by cold enrichment in campy-thio broth.isolation of campylobacter jejuni from human feces by direct inoculation to campy-bap (scott laboratories, inc., fiskeville, r.i.) was compared with isolation after overnight enrichment at 4 degrees c in campy-thio broth followed by subculture to campy-bap. of 54 positive specimens, 19 were positive only after enrichment, and 5 were positive only on the direct plate. among 36 positive patients, 10 were detected by enrichment only and 2 by direct plating only. laboratories using campy-bap should ...19836630456
effect of ferrous sulfate, sodium metabisulfite, and sodium pyruvate on survival of campylobacter jejuni.a combination of ferrous sulfate, sodium metabisulfite and sodium pyruvate, incorporated in solid medium, maintained the characteristic morphology, motility, and viability of six isolates of campylobacter jejuni stored at room temperature and 4 degrees c for up to 20 and 30 days, respectively, under normal atmospheric conditions.19836630476
campylobacter fetus subsp. fetus in homosexual males.campylobacter fetus subsp. fetus was isolated from the stools of two homosexual males. one was asymptomatic at the time of isolation. the other presented with diarrhea. both isolates were initially grown at 42 degrees c. this organism should be included among the list of organisms that are found in homosexual males.19836630480
demonstration of a cytotoxin from campylobacter jejuni.a 48-hour culture filtrate of campylobacter jejuni was found to produce cytopathic effects on three human cell lines--that is, hela, mrc-5 and hep-2. the cytopathic effects observed include cell rounding, loss of adherence and cell death after 24-48 h of incubation. such morphological changes were observed with eight of the eleven strains of campylobacter jejuni isolated from the blood/stools of patients who suffered from either acute gastroenteritis or septicaemia. the toxic factor did not reta ...19836630575
molecular identification of surface protein antigens of campylobacter jejuni.the technique of immunoblotting was used to identify the surface protein antigens of campylobacter jejuni. polyclonal antisera were raised in rabbits to formalinized cells of a typical human fecal isolate, c. jejuni vc74. surface components were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. fractions analyzed included whole cell lysates, sarcosinate-extracted outer membranes, released outer membrane blebs (fragments), isolated flagella, 0.2 m glycine-hydrochloride (ph 2 ...19836642648
simple adult rabbit model for campylobacter jejuni enteritis.we tested the usefulness of the removable intestinal tie adult rabbit diarrhea model to establish campylobacter jejuni infection in rabbits. the procedure involved ligation of the cecum, placement of a slip knot at the terminal ileum, and injection of the test inoculum into the mid-small bowel. the ends of the slip knot were externalized, and the tie was released 4 h later. fifty-five rabbits received c. jejuni, and 16 received uninoculated medium as controls. daily rectal swabs were positive fo ...19836642664
campylobacter fetus ssp. fetus bacteremia in a patient with liver cirrhosis. 19836642681
probable campylobacter fetus subsp. fetus gastroenteritis.three strains of campylobacter fetus subsp. fetus isolated from cases of gastroenteritis are reported. dna-dna hybridizations in addition to biochemical tests were used to confirm the identification of the isolates as c. fetus since all strains grew at 42 degrees c. these isolates, like other c. fetus strains, are susceptible to cephalothin and thus would not have been detected in laboratories with campylobacter isolation media containing this component.19836643676
the isolation of campylobacter jejuni from flies.living flies collected from three locations were cultured on selective medium for campylobacter spp. campylobacter jejuni was isolated from five (2.4%) of 210 flies examined. these results suggest that the potential hazard to health from the transmission of campylobacters from animals to human food by flies is small.19836644008
milk-borne campylobacter enteritis in a rural area.during november and december 1981 more than 50 residents in a village in derbyshire had an acute gastrointestinal illness. one month later a second outbreak occurred affecting another 22 people. campylobacter jejuni was isolated from 12 patients; no other gastrointestinal pathogens were identified. a case-control study showed an association with the consumption of unpasteurized milk from one particular farm. no new cases were identified for 6 months following the application of a pasteurization ...19836644009
[ulcerative colitis and campylobacter fetus ss. jejuni]. 19836645707
morphological differences in flagella in campylobacter fetus subsp. intestinalis and c. fetus subsp. jejuni.intact flagella were isolated from human pathogenic strains of campylobacter, c. fetus subsp. intestinalis and c. fetus subsp. jejuni, by the method of depamphilis and adler and examined by electron microscopy. the isolated flagella were composed of a filament, a hook, a basal body, and a large disk associated with the end of the hook region covering the basal body. the width of the hook was approximately 28 nm, somewhat greater than that of the filament (20 nm in diameter). the hook region of c ...19836645983
medium for isolating campylobacter fetus. 19836649358
isolation and enumeration of campylobacter jejuni from poultry products by a selective enrichment method.a direct selective enrichment procedure was developed for the isolation of campylobacter jejuni from poultry products. the selective enrichment medium (atb) consisted of (per liter) tryptose (20 g), yeast extract (2.5 g), sodium chloride (5 g), fbp supplement (ferrous sulfate [0.25 g], sodium metabisulfite [0.25 g], sodium pyruvate [0.25 g]), bicine (10 g), and agar (1 g). hematin solution (6.25 ml; prepared by dissolving 0.032 g of bovine hemin in 10 ml of 0.15 n sodium hydroxide solution and a ...19836651294
investigation of a waterborne outbreak of campylobacter jejuni enteritis with a serotyping scheme based on thermostable antigens.serotyping of 11 human and 2 water isolates of campylobacter jejuni associated with a waterborne outbreak revealed two serotypes among the human isolates. one of these (serotype 58) was a new serotype and was added to the serotyping scheme. serotypes were defined by using extracted thermostable antigens and passive hemagglutination titrations of both unabsorbed and cross-absorbed antisera. two water isolates of the same serotype as six human isolates provided evidence to link a contaminated wate ...19836655043
detection of menaquinone-6 and a novel methyl-substituted menaquinone-6 in campylobacter jejuni and campylobacter fetus subsp. fetus.menaquinone-6 (2-methyl-3-farnesyl-farnesyl-1,4-naphthoquinone) and a methyl-substituted menaquinone-6 (2,[5 or 8]-dimethyl-3-farnesyl-farnesyl-1,4-naphthoquinone) were the major isoprenoid quinones found in membrane preparations of campylobacter jejuni and campylobacter fetus subsp. fetus. by reverse-phase high-performance liquid chromatography (hplc) and thin-layer chromatography (tlc) the faster-eluting menaquinone-6 co-chromatographed with a menaquinone-6 standard. the identity of menaquinon ...19836663282
[neonatal meningitis caused by campylobacter fetus]. 19836674590
[campylobacter infection and the hemolytic-uremic syndrome].a 20-year-old man with an acute enteritis caused by campylobacter fetus developed a haemolytic-uraemic syndrome on the fifth day of his disease. the pathogenesis of the disseminated intravascular coagulopathie , the nephropathie and the haemolysis are discussed. with increasing rate of isolation of campylobacter, the connection of this infection with hus may be seen more frequently.19836676172
[isolation of campylobacter fetus ssp. jejuni from products of animal origin]. 19836680922
mycotic aortic aneurysm. a complication of campylobacter fetus septicemia.the first surviving case, to our knowledge, of a campylobacter fetus mycotic aortic aneurysm is reported. bacteremia and an ileofemoral thrombophlebitis preceded the development of the infected aneurysm, reconfirming the vascular tropism of this organism. the clinical similarity with infections caused by salmonella choleraesuis is illustrated by this case. the full recovery of our patient attests to the efficacy of extralanatomic bypass combined with long-term antibiotic therapy in the treatment ...19836687525
infection with campylobacter fetus.over a 4-yr period (1978-1982) about 1 000 cases of campylobacter infection were diagnosed at our laboratory in helsinki, but only 4 proved to be due to campylobacter fetus. all these 4 patients had fever, 3 had diarrhoea and 1 arthritis and thrombophlebitis. the diagnosis was made from blood cultures in all cases; in 1 case the bacterium was also isolated from stools.19846695155
endemic campylobacter jejuni infection in colorado: identified risk factors.a study of persons with laboratory-confirmed sporadic campylobacter jejuni infection and of controls matched for age and sex in colorado in the summer of 1981 yielded odds ratio estimates significantly greater than 1 for the following risk factors: drinking raw water (10.74), drinking raw milk (6.93), eating undercooked chicken (2.77), and living in a household with a cat (3.21).19846696155
bacterial etiology of diarrhea in children with special reference to campylobacter fetus jejuni. 19846698587
campylobacter jejuni diarrhea model in infant chickens.to study the pathogenic mechanisms of campylobacter jejuni infection, 36- to 72-h-old chickens were fed 10(3) to 10(6) live cells, using strains isolated from 40 patients with watery diarrhea and 6 with bloody mucoid diarrhea from whom no other known enteropathogen was detected. chickens of starbro strain were more likely to develop c. jejuni-induced diarrhea than were white leghorn chickens. diarrhea was defined on the basis of amounts of gut fluid in 288 chicks fed with live c. jejuni versus 1 ...19846698612
serotyping and biotyping of campylobacter jejuni and campylobacter coli from sporadic cases and outbreaks in norway.of 172 thermophilic campylobacters isolated from human cases of gastroenteritis in norway, 149 (86.6%) were classified as campylobacter jejuni, whereas 23 isolates (13.4%) belonged to campylobacter coli. c. jejuni biotype 1 comprised 66.3% and c. jejuni biotype 2 comprised 20.3% of the total number. using 50 unabsorbed antisera, we were able to serotype 109 (80.1%) of 136 campylobacters on the basis of heat-stable antigens identified by means of passive hemagglutination. the typable strains fell ...19846699144
blood-free selective medium for isolation of campylobacter jejuni from feces.a blood-free selective agar is described which contains charcoal, ferrous sulfate, sodium pyruvate, casein hydrolysates, cefazolin, and sodium deoxycholate (ccd agar). ccd agar was compared with preston medium for isolation of campylobacter jejuni from human feces, and isolation rates were similar on both media, but ccd agar was less selective. temperature studies at 37 and 42 degrees c confirmed that incubation of direct plates at 42 degrees c for 48 h was necessary for maximum isolation of c. ...19846699146
improved preservation medium for campylobacter jejuni.an egg-based medium was found to be superior to the conventional wang transport medium and the recently developed biphasic medium for the preservation of campylobacter jejuni in the laboratory. strains of c. jejuni preserved in egg-based medium maintained at 4 degrees c were viable for over 3 months. the survival of c. jejuni in egg-based medium held at room temperature (27 +/- 2 degrees c) was also relatively longer than in wang transport medium and biphasic medium.19846699154
in vitro susceptibilities of 40 campylobacter fetus subsp. jejuni strains to niridazole and metronidazole.the activities of niridazole and metronidazole were compared by an agar dilution method against 40 strains of campylobacter fetus subsp. jejuni of human origin. niridazole had a markedly higher activity than metronidazole.19846703680
[frequency of campylobacter fetus ss. jejuni and yersinia enterocolitica in children with acute diarrhea]. 19846704228
diagnostic studies of the fetus, placenta and maternal blood from 265 bovine abortions.during 1981, the fetus, placenta and maternal serum were received from each of 265 bovine abortions. these specimens were examined using histopathological, histochemical, bacteriological, mycological, endocrinological, immunological, serological and virological techniques. the cause of abortion was identified in 98 (37%) cases. of these diagnosed abortions 27 (28%) were due to infection with fungi, 17 (17%) to salmonella spp, 11 (11%) to campylobacter fetus and 10 (10%) to corynebacterium pyogen ...19846705541
association of campylobacter jejuni with laying hens and eggs.laying hens were individually caged at 20 weeks of age and tested for fecal excretion of campylobacter jejuni (minimum level of detection was 100 cfu/g) during a 42-week period. peak rates of c. jejuni isolation (approximately 25% of hens positive) occurred at two different times, in october and in late april to early may. before being segregated in late september, birds were allowed to consume fecal matter, litter, and communal drinking water, all likely sources of c. jejuni. the increased excr ...19846712220
hemolytic uremic syndrome after campylobacter-induced diarrhea in an adult.campylobacter fetus subspecies jejuni is a recognized pathogen of the gastrointestinal (gi) tract resulting in a spectrum of illness from mild gastroenteritis to severe colitis with bloody diarrhea. campylobacter is also being recognized as capable of producing systemic illness. furthermore, antibody response, hypocomplementemia, and bacteremia with enterotoxic organisms have been described. many of the clinical features, both local (le, in the gi tract) and systemic, parallel those of shigella. ...19846712399
comparative efficacy of liquid enrichment medium for isolation of campylobacter jejuni.isolation of campylobacter jejuni from 1,249 human fecal specimens by direct inoculation on selective columbia agar and liquid enrichment medium was compared. the use of liquid enrichment medium provided a 30% higher isolation rate of c. jejuni. the overall isolation rate achieved by using direct plating and enrichment together was 8.2%.19846715514
comparative studies on competitive exclusion of three isolates of campylobacter fetus subsp. jejuni in chickens by native gut microflora.resistance of young chicks to campylobacter fetus subsp. jejuni was substantially increased by early exposure to native gut microflora. protection was demonstrated against two human isolates and a chicken isolate of c. fetus subsp. jejuni. significant protection against the chicken isolate was observed throughout a 91-day test period. infection reached 100% (25/25) in the untreated group at 56 days of age and only 4% (1/25) in the group treated with native gut microflora. campylobacter fetus sub ...19846721792
is enrichment culture necessary for the isolation of campylobacter jejuni from faeces? 19846725607
a study of the spread of campylobacter jejuni in four large kitchens.campylobacters were sought in swabs taken from work surfaces, sinks and floors of four kitchens-i.e. hospital, university, cook-freeze and commercial, processing frozen or fresh chickens. each kitchen was visited on four occasions. in the large commercial kitchen environmental contamination was found on each visit, whereas campylobacters were isolated on six of the twelve visits to the other kitchens. the hands of operatives were contaminated with campylobacters on only two of the 45 swabs taken ...19846736643
[a case of septicemia by campylobacter fetus subsp. fetus]. 19846737735
campylobacter septic abortion.we have reported a case of a septic abortion caused by campylobacter fetus ssp jejuni. this organism is best known for producing diarrhea, but is now being linked with more serious diseases, including pregnancy wastage.19846740362
[an outbreak of campylobacter fetus subspecies jejuni enteritis in newborn infants in a maternity hospital]. 19846742699
properties of crude campylobacter jejuni heat-labile enterotoxin.the amount of crude campylobacter jejuni enterotoxin present in culture products was quantitated by comparing the response of these preparations with that of pure escherichia coli heat-labile toxin (lt) in the chinese hamster ovary assay and in enzyme-linked immunosorbent assays that used gm ganglioside or antisera to lt or both. maximum c. jejuni enterotoxin production was achieved by growth at 42 degrees c for 24 h under agitation in supplemented gc medium. adding polymyxin separately to eithe ...19846746090
dna relatedness among strains of campylobacter jejuni and campylobacter coli with divergent serogroup and hippurate reactions.eleven strains of campylobacter from earlier fluorescent-antibody studies were examined by dna hybridization to determine their species. three of the strains hydrolyzed sodium hippurate, and eight did not. four of the hippurate-negative strains were in campylobacter jejuni serogroups, and the remaining strains were in both c. jejuni and campylobacter coli serogroups. dna relatedness to type strains of c. jejuni and c. coli indicated that all three of the hippurate-positive strains and two of the ...19846746886
systematic investigation of enrichment media for wild-type campylobacter jejuni strains.of the media examined, thioglycolate broth supplemented with 5% lysed sheep blood, butzler antibiotic mixture, and 0.1% lauryl sulfate was the most sensitive enrichment medium for recovery of wild-type strains of campylobacter jejuni from cecal contents of chickens and chicken livers. it allowed the retrieval of 1 cfu as did solid media but permitted the screening of 50-times larger volumes. double-strength enrichment medium required 5 to 10 cfu for growth. omission of lauryl sulfate reduced the ...19846746890
factors affecting the lethality of campylobacter fetus subspecies jejuni in mice.intraperitoneal injection of campylobacter fetus ss. jejuni into ham/1cr mice was lethal, but viable counts of bacteria from whole body homogenates, organs and blood indicated that death was not due to sustained bacterial multiplication. heat-killed organisms (5 x 10(9) cfu) injected into 7-day-old mice caused death within 24 h and this was shown to be due to endotoxin. both ferric iron and heterologous lipopolysaccharide enhanced virulence; the ld50 was lowered from 1.8 x 10(9) cfu to 2.7 x 10( ...19846748038
comparison of campypak ii with standard 5% oxygen and candle jars for growth of campylobacter jejuni from human feces.to determine optimal temperature and atmospheric conditions for isolating campylobacter jejuni from fecal specimens of humans, we studied six laboratory isolates and 19 fecal specimens that were known to contain c. jejuni. we compared incubations in 5% oxygen, the campypak ii (bbl microbiology systems, cockeysville, md.) with 6 plates per jar (cp-6) and 12 plates per jar (cp-12), and candle jars at 37 and 42 degrees c. at both temperatures, the colony sizes for the laboratory strains were larger ...19826749892
a most probable number method for estimating small numbers of campylobacters in water.a most probable number (mpn) method capable of estimating as few as ten campylobacters per 100 ml of water is described. the method gave results close to those obtained by the viable count method of miles, misra & irwin (1938) with graded suspensions of campylobacter jejuni. the method was used to test raw water samples: counts were obtained ranging from 10 to 230 campylobacters per 100 ml for 11 49 coastal and estuary water samples, and from 10 to 36 campylobacters per 100 ml for 7 of 44 river ...19826752269
[cultural demonstration of campylobacter fetus subspecies fetus in preputial rinsing samples from insemination and breeding bulls]. 19826758761
[recent studies on campylobacter fetus jejuni]. 19826764587
enrichment medium and control system for isolation of campylobacter fetus subsp. jejuni from stools.enrichment culture with a semisolid medium increased by 6% the isolation rate of campylobacter fetus subsp. jejuni. the semisolid enrichment medium was also used successfully as a transport medium for campylobacter isolates. a blood agar plate streaked with pseudomonas aeruginosa, clostridium perfringens, and a laboratory strain of campylobacter was a good control system for the microaerophilic atmosphere. good growth of all three organisms indicated satisfactory conditions for culturing campylo ...19826764764
evaluation of the campypak ii gas generator system for isolation of campylobacter fetus subsp. jejuni.the campypak ii (bbl microbiology systems, cockeysville, md.) method for isolating campylobacter fetus subsp. jejuni was evaluated with clinical specimens by comparison with an evacuation replacement procedure. of 757 specimens, 26 (3.4%) were positive for c. fetus subsp. jejuni. all 26 were recovered by both systems. no difference was found in the time necessary for isolation, except with one isolate that required 3 days for isolation with campypak ii and 2 days with the established procedure. ...19826764774
comparison of atmospheres of incubation for primary isolation of campylobacter fetus subsp. jejuni from animal specimens: 5% oxygen versus candle jar.an atmosphere with reduced oxygen tension is required for the primary isolation of campylobacter fetus subsp. jejuni. therefore, we compared use of the conventional atmosphere of 5% oxygen and 8% carbon dioxide with use of a candle jar (17% oxygen and 3% carbon dioxide) for primary isolation of c. fetus subsp. jejuni from 263 positive canine, cattle, and turkey fecal or cecal specimens. at an incubation temperature of 42 degrees c, the atmosphere with 5% oxygen resulted in more campylobacter col ...19826764778
dark-field microscopy of human feces for presumptive diagnosis of campylobacter fetus subsp. jejuni enteritis.to determine the value of direct dark-field microscopy for diagnosing enteritis due to campylobacter fetus subsp. jejuni, we examined 1,377 human fecal specimens for bacteria with typical campylobacter darting motility, leukocytes, and erythrocytes. eighty-four specimens (6.1%) grew c.fetus subsp. jejuni. of the 48 specimens showing campylobacter motility, 30 (62%) grew c. fetus subsp. jejuni. the sensitivity, specificity, and predictive value of observing campylobacter motility were 36%, 99%, a ...19826764779
[cases of gastroenteritis caused by campylobacter fetus jejuni in vienna].campylobacter fetus jejuni is a gram-negative spiral or comma-shaped rod, belonging to the family of spirillaceae. it is now recognised as a cause of human gastroenteritis with fever. recently, many reports have been published about sporadic cases as well as epidemic outbreaks. a symptomless carrier-state is also known. the mode of transmission is not yet fully understood. direct, faecal-oral and indirect transmission especially via food contaminated with faeces are suspected. recently, we isola ...19806765653
campylobacter fetus subspecies jejuni: the need for surveillance. 19806768813
[diarrheal disease associated with campylobacter fetus subspecies jejuni (author's transl)]. 19806768818
plasma progesterone concentration in the bovine before abortion or parturition in pregnant animals exposed to sarcocystis cruzi, campylobacter fetus, or aspergillus fumigatus.pregnant cows in the 4th and 5th month of the gestation were exposed to sarcocystis cruzi, campylobacter fetus, or aspergillus fumigatus. plasma progesterone concentrations were determined at intervals from before the cows were exposed until they had aborted or calved. the plasma progesterone concentration of the exposed pregnant cattle remained at 3.95 +/- 2.0 ng/ml until 24 to 48 hours before abortion or parturition, when it decreased to below 1.00 ng/ml. this pattern was similar for cattle ex ...19806782916
prepartum changes of plasma concentrations of prostaglandin f and 13,14-dihydro-15-ketoprostaglandin metabolites in pregnant animals exposed to sarcocystis cruzi or campylobacter fetus.pregnant cows at 4- to 5-months' of gestation were exposed to sarcocystis cruzi or campylobacter fetus. plasma prostaglandin f (pgf) and 13,14-dihydro-15-ketoprostaglandin metabolite (pgm) concentrations were determined at intervals from before exposure until abortion or parturition. the plasma pgf concentration of pregnant infected cattle remained at 0.02 +/- 0.04 ng/ml until 24 to 48 hours before abortion or parturition when it increased 5-fold to 0.11 +/- 0.12 ng/ml. the plasma pgm concentrat ...19816784618
acute ulceration of ileal stoma due to campylobacter fetus subspecies jejuni. 19816786499
milk-borne campylobacter infection.the common factor in 13 recent outbreaks of campylobacter jejuni enteritis was the consumption of unpasteurised or incompletely pasteurised milk. c jejuni is a common commensal in the alimentary tract of milking cows, but it is not clear how the milk becomes contaminated with the organism. pasteurisation will readily eliminate the organism from milk. in england and wales 3% of milk retailed is still unpasteurised, and in the light of these findings it is suggested that only pasteurised milk shou ...19816786504
infective dose of campylobacter jejuni in milk. 19816786571
passive hemagglutination technique for serotyping campylobacter fetus subsp. jejuni on the basis of soluble heat-stable antigens.antigenic materials were extracted from campylobacter fetus subsp. jejuni strains by heating bacterial suspensions in saline at 100 degrees c and by exposure to ethylenediaminetetraacetic acid. the antigens were heat stable at 100 degrees c, capable of sensitizing sheep erythrocytes for agglutination in antisera, and able to elicit production of specific antibody in rabbits; they occurred with different immunological specificities in 23 strains. antisera against the 23 strains could be used for ...19806796598
isolation of campylobacter fetus subsp jejuni from zoo animals.over a 1-year period, 619 fecal specimens from animals at the denver zoo were cultured for campylobacter fetus subsp jejuni. the organism was isolated from 35 animals, including 12 primates, 2 felids, a red panda, 13 hooved animals, 6 birds, and 1 reptile. of 44 cultured fecal specimens from diarrheal animals, 31.8% were positive for campylobacter, whereas only 5.6% of 575 specimens from animals without diarrhea were positive (p less than 0.001). among 25 isolates tested, 12 serotypes were repre ...19816799468
campylobacter jejuni survival in chicken meat as a function of temperature.recognition of campylobacter fetus subsp. jejuni (referred to hereafter as c. jejuni) as an important human pathogen and its isolation from meat products indicate the need for knowledge of its survival characteristics in meats. thermal death times (d-values) for a single strain and a five-strain composite were determined in 1% peptone and autoclaved ground chicken meat at temperatures ranging from 49 to 57 degrees c. survival was determined for these strains in chicken meat at 4, 23, 37, and 43 ...19826812501
comparison of selective media for isolation of campylobacter jejuni/coli.a comparison of skirrow's, butzler's, blaser's, campy-bap and preston media for campylobacter spp was made using human, animal and environmental specimens. butzler's medium gave the lowest isolation rate and preston medium, which was the most selective, the highest isolation rate. enrichment culture using preston enrichment broth gave a higher isolation rate than direct plating onto preston medium.19836822680
inflammatory abdominal aortic aneurysm infected by campylobacter fetus. 19836823082
campylobacter enteritis in 188 hospitalized patients.the clinical picture and epidemiologic characteristics of infection due to campylobacter fetus subspecies jejuni were studied in 188 patients hospitalized in finland during a three-year period. all but two patients had diarrhea; 90% had abdominal pain, fever, and fatigue; half had vomiting and headache; one third experienced electrolyte disturbances; and one fifth of the patients had other complications, most commonly pancreatitis (6%) and arthritis (5%). all age groups were affected, most usual ...19836824388
relapsing septicemia caused by campylobacter fetus subsp. fetus. 19836825036
prevalence of campylobacter jejuni in two california chicken processing plants.two federally inspected california chicken processing plants participated in campylobacter jejuni prevalence studies. twelve sampling sites were included in each of four groups. groups were based on bird age, scald water temperature, and plant sampled. scald water temperatures of 60 degrees c (140 degrees f) did not contribute to a lower prevalence of c. jejuni in edible parts, as did temperatures of 53 degrees c (127 degrees f) and 49 degrees c (120 degrees f). the feather picker and chilling t ...19836830212
house flies (musca domestica) as possible vectors of campylobacter fetus subsp. jejuni.a total of 161 strains of campylobacter fetus subsp. jejuni were isolated from house flies (musca domestica). the carrier rates detected were 50.7% in flies captured on a chicken farm and 43.2% in flies from a piggery. the relative prevalences of campylobacter coli, c. jejuni, and nalidixic acid-resistant thermophilic campylobacters were 90.1, 6.2, and 3.7%, respectively. the results indicate that flies may play a linking role in the epidemiology of campylobacter infection in humans by transmitt ...19836830213
experimental campylobacter jejuni infection of adult mice.ha-icr adult mice were studied to develop an animal model for campylobacter jejuni enteritis in humans. fecal and ileal cultures made by selective and nonselective methods showed that c. jejuni and related organisms are not bowel commensals. intragastric feeding of 10(8) cfu of three different strains of c. jejuni produced infection in 100% of the animals, and infection rates were dose dependent. pretreatment with antibiotics or opiates was not necessary to induce infection. fresh isolates and s ...19836832823
culture supernatants of campylobacter jejuni induce a secretory response in jejunal segments of adult rats.culture supernatants of four campylobacter jejuni strains induced a net sodium secretory flux (plasma-lumen) and an impaired glucose transport in perfused jejunal segments of adult rats in vivo.19836832835
the association of malabsorption syndrome with a campylobacter fetus infection. 19836833780
[campylobacter fetus bacteremia].five cases of campylobacter fetus bacteremia are reported. this germ, found in blood cultures, induces high fever and is accompanied by either gastroenteritis with colitis or thrombophlebitis. other, but much rarer septic sites are the meninges and endocardium. patients already debilitated by chronic disease are more susceptible to bacteremia with campylobacter fetus, which worsens the prognosis of the infection. although non-debilitated patients can contract this infection, the prognosis here i ...19836836251
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