Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| new connections in the prokaryotic toxin-antitoxin network: relationship with the eukaryotic nonsense-mediated rna decay system. | several prokaryotic plasmids maintain themselves in their hosts by means of diverse post-segregational cell killing systems. recent findings suggest that chromosomally encoded copies of toxins and antitoxins of post-segregational cell killing systems - such as the rele system - might function as regulatory switches under stress conditions. the rele toxin cleaves ribosome-associated transcripts, whereas another post-segregational cell killing toxin, pare, functions as a gyrase inhibitor. | 2003 | 14659018 |
| [pathogenesis of campylobacter fetus infection]. | campylobacter fetus subsp. fetus is a zoonotic pathogen and cause opportunistic systemic infection in human who either are pregnant or have an underlying illness. crystalline surface layer (s-layer), an external structure on the outer membrane is the prime virulence factor of this organism. s-layer is responsible for resistance to both leukocyte phagocytosis and the bactericidal effects of human serum. antigenic variation of c. fetus surface antigen during infection also help the organism to esc ... | 2003 | 14509230 |
| a survey of campylobacter species shed in faeces of beef cattle using polymerase chain reaction. | a polymerase chain reaction (pcr)-based survey of campylobacters associated with faeces collected from 382 beef cattle was undertaken. to ensure the removal of pcr inhibitors present in faeces and determine if adequate extraction was achieved, faeces were seeded with internal control dna (i.e., dna designed to amplify with the campylobacter genus primer set, but provide a smaller amplicon) before the extraction procedure. in only two samples (0.5%) were the internal control or campylobacter genu ... | 2003 | 14735214 |
| aetiology of bovine abortion in argentina. | necropsies were performed on 354 fetuses from dairy and beef herds submitted from 1994 to 2000 to the diagnostic laboratories at instituto nacional de tecnología agropecuaria, balcarce, argentina. samples from the fetuses were examined for pathogenic organisms and processed for microscopic examination. an aetiological diagnosis was made for 161 (45.5%) of the fetuses. no diagnosis was made for 193 (54.5%) fetuses. infectious agents were isolated from 122 (34.4%) of the fetuses, bacterial agents ... | 2003 | 14509450 |
| comparative molecular and microbiologic diagnosis of bacterial endocarditis. | sequencing of 16s rdna, and of sodaint and rpobint in some cases, was applied to dna from heart valves of 46 patients (36 with definite and 10 with possible endocarditis). sequence-based identifications were compared with those obtained with conventional methods. among the 36 definite cases, 30 had positive blood cultures and 6 had negative cultures. among the 30 positive cases, sequencing of 16s rdna permitted identification of species (18), genus (8), or neither (4); sodaint and rpobint sequen ... | 2003 | 14720393 |
| pulsed-field gel electrophoresis typing of campylobacter fetus subsp. fetus from sheep abortions in the hawke's bay region of new zealand. | to type campylobacter isolates from sheep abortions from the hawke's bay region of new zealand. | 2003 | 16032287 |
| [campylobacter fetus bacteremia in immunocompetent patient]. | 2003 | 14516269 | |
| evaluation of acridine orange staining as a replacement of subcultures for bact/alert-positive, gram stain-negative blood cultures. | among 18,424 blood culture sets processed during a study period of 18 months, 85 bottles that were positive by the bact/alert system were gram stain negative. both acridine orange staining and subcultures detected microorganisms in a total of 12 bottles. acridine orange staining can replace subcultures of false-positive blood cultures. | 2003 | 14605172 |
| comparison of recovery of blood culture isolates from two bact/alert fan aerobic blood culture bottles with recovery from one fan aerobic bottle and one fan anaerobic bottle. | traditionally, a routine blood culture for adult patients consisted of paired aerobic and anaerobic bottles, but the routine use of an anaerobic blood culture bottle has been challenged in recent years. in this study, we compared the recovery of two fan aerobic bottles with one fan aerobic and one fan anaerobic bottle. each pair of bottles was collected by a separate collection procedure, and each bottle held a recommended 8- to 12-ml draw. a total of 704 clinically significant isolates were rec ... | 2003 | 12517851 |
| specific detection of arcobacter and campylobacter strains in water and sewage by pcr and fluorescent in situ hybridization. | the aim of this study was to evaluate pcr and fluorescent in situ hybridization (fish) techniques for detecting arcobacter and campylobacter strains in river water and wastewater samples. both 16s and 23s rrna sequence data were used to design specific primers and oligonucleotide probes for pcr and fish analyses, respectively. in order to assess the suitability of the methods, the assays were performed on naturally and artificially contaminated samples and compared with the isolation of cells on ... | 2003 | 12571045 |
| pcr-restriction fragment length polymorphism analysis for detection of point mutations associated with macrolide resistance in campylobacter spp. | a 23s rrna gene fragment in domain v was sequenced from 30 clinical isolates of campylobacter spp., including 22 resistant to macrolides. two point mutations associated with erythromycin resistance were identified at positions 2074 and 2075 on the 23s rrna gene (homologous to a2142c and a2143g mutations in helicobacter pylori) in which an adenine residue is also replaced with a cytosine and a guanine residue, respectively. a combined pcr-restriction fragment length polymorphism technique was dev ... | 2003 | 12604552 |
| a real-time pcr assay for the detection of campylobacter jejuni in foods after enrichment culture. | a real-time pcr assay was developed for the quantitative detection of campylobacter jejuni in foods after enrichment culture. the specificity of the assay for c. jejuni was demonstrated with a diverse range of campylobacter species, related organisms, and unrelated genera. the assay had a linear range of quantification over six orders of magnitude, and the limit of detection was approximately 12 genome equivalents. the assay was used to detect c. jejuni in both naturally and artificially contami ... | 2003 | 12620820 |
| evaluation of 11 pcr assays for species-level identification of campylobacter jejuni and campylobacter coli. | we examined the sensitivity and specificity of 11 pcr assays described for the species identification of campylobacter jejuni and campylobacter coli by using 111 type, reference, and field strains of c. jejuni, c. coli, and campylobacter lari. for six assays, an additional 21 type strains representing related campylobacter, arcobacter, and helicobacter species were also included. pcr tests were initially established in the laboratory by optimizing conditions with respect to five type and referen ... | 2003 | 12517869 |
| epidemiology and antimicrobial susceptibilities of 111 campylobacter fetus subsp. fetus strains isolated in québec, canada, from 1983 to 2000. | the epidemiology and antimicrobial susceptibilities of 111 campylobacter fetus subsp. fetus strains isolated from 103 patients from 1983 to 2000 in québec, canada, were determined. the median number of patients infected annually with this bacteria was seven, with an incidence of 0.1 per 100,000 population. the male-to-female ratio was 1.1 to 1.0. the patients originated from 13 of the 18 québec socioeconomic regions. the age range of the patients was 6 months to 90 years old, 53% being > or = 70 ... | 2003 | 12517895 |
| usefulness of the microseq 500 16s ribosomal dna-based bacterial identification system for identification of clinically significant bacterial isolates with ambiguous biochemical profiles. | due to the inadequate automation in the amplification and sequencing procedures, the use of 16s rrna gene sequence-based methods in clinical microbiology laboratories is largely limited to identification of strains that are difficult to identify by phenotypic methods. in this study, using conventional full-sequence 16s rrna gene sequencing as the "gold standard," we evaluated the usefulness of the microseq 500 16s ribosomal dna (rdna)-based bacterial identification system, which involves amplifi ... | 2003 | 12734240 |
| phenotypic and genotypic differentiation of campylobacter spp. isolated from austrian broiler farms: a comparison. | an identification scheme based on restriction fragment length polymorphism of polymerase chain reaction products (pcr-rflp) was developed to differentiate isolates of the genera campylobacter, arcobacter and helicobacter. based on the 16s rrna gene of these genera, pcr amplified a 1216-bp fragment. the amplicons were digested with the restriction enzymes rsai and ecorv. additional differentiation was obtained using a pcr-assay based on the hippuricase gene. genotyping was performed on several re ... | 2003 | 12745378 |
| use of pcr for direct detection of campylobacter species in bovine feces. | this study reports on the use of pcr to directly detect and distinguish campylobacter species in bovine feces without enrichment. inhibitors present in feces are a major obstacle to using pcr to detect microorganisms. the qiaamp dna stool minikit was found to be an efficacious extraction method, as determined by the positive amplification of internal control dna added to bovine feces before extraction. with nested or seminested multiplex pcr, campylobacter coli, c. fetus, c. hyointestinalis, and ... | 2003 | 12788747 |
| rapid detection of campylobacter jejuni in chicken rinse water by melting-peak analysis of amplicons in real-time polymerase chain reaction. | five dna extraction protocols for the detection of campylobacter spp. by polymerase chain reaction (pcr) were compared. a method involving triton x-100 produced template dna of sufficient quality to allow the detection of campylobacter jejuni at levels of 100 cfu/ml in pure culture. primers were designed on the basis of the cadf gene sequence. with a sybr green i real-time pcr assay, these primers amplified only sequences present in c. jejuni to produce a product with a melting temperature of 81 ... | 2003 | 12929818 |
| toward an international standard for pcr-based detection of food-borne thermotolerant campylobacters: validation in a multicenter collaborative trial. | as part of a european research project, the performance of a pcr assay to detect food-borne thermotolerant campylobacters (campylobacter jejuni, c. coli, and c. lari) was evaluated through an international collaborative trial involving 12 participating laboratories. dna from 10 target and 8 nontarget strains was tested, and the results were reported as the presence of a positive signal after gel electrophoresis. the overall inclusivity (sensitivity) was 93.7%, and the exclusivity (specificity) w ... | 2003 | 12957959 |
| role of s-layer protein antigenic diversity in the immune responses of sheep experimentally challenged with campylobacter fetus subsp. fetus. | surface layer proteins (slps) are essential for induction of abortion by campylobacter fetus subsp. fetus in experimentally challenged ewes. these proteins are encoded by multiple sap genes and vary in size and antigenicity. the role of slp antigenic variation during experimental ovine infection was investigated. following subcutaneous challenge, the slps were highly antigenic, and antibodies were detected in serum, milk, bile, and urine. fecal anti-slp antibodies were detected only in animals c ... | 2003 | 12496160 |
| cd4+cd25+ t(r) cells suppress innate immune pathology through cytokine-dependent mechanisms. | cd4(+)cd25(+) regulatory t (t(r)) cells can inhibit a variety of autoimmune and inflammatory diseases, but the precise mechanisms by which they suppress immune responses in vivo remain unresolved. here, we have used helicobacter hepaticus infection of t cell-reconstituted recombination-activating gene (rag)(-/-) mice as a model to study the ability of cd4(+)cd25(+) t(r) cells to inhibit bacterially triggered intestinal inflammation. h. hepaticus infection elicited both t cell-mediated and t cell ... | 2003 | 12515818 |
| rapid detection of methicillin-resistant staphylococcus aureus directly from sterile or nonsterile clinical samples by a new molecular assay. | a rapid procedure was developed for detection and identification of methicillin-resistant staphylococcus aureus (mrsa) directly from sterile sites or mixed flora samples (e.g., nose or inguinal swabs). after a rapid conditioning of samples, the method consists of two main steps: (i) immunomagnetic enrichment in s. aureus and (ii) amplification-detection profile on dna extracts using multiplex quantitative pcr (5'-exonuclease qpcr, taqman). the triplex qpcr assay measures simultaneously the follo ... | 2003 | 12517857 |
| pseudobacteraemia in a patient with neutropenic fever caused by a novel paenibacillus species: paenibacillus hongkongensis sp. nov. | to characterise a strain of gram negative aerobic straight or slightly curved rods (hku3) isolated from the blood culture of a 9 year old chinese boy with neutropenic fever and pseudobacteraemia. | 2003 | 12560460 |
| [a man with campylobacter endocarditis, treatable as campylobacter fetus following identification]. | a 48-year-old dutch patient presented with general malaise, recurrent fever and weight loss. routine cultures identified a campylobacter in two blood cultures. when initial treatment with clarithromycin failed, the patient developed endocarditis. dna sequencing identified campylobacter fetus subspecies fetus (c. fetus). the patient recovered after treatment of the infection with imipenem and gentamycin, based on the minimal inhibiting concentration. c. fetus is a rare cause of infection in human ... | 2003 | 12661460 |
| [identification and differentiation of campylobacter fetus subspecies by pcr]. | the species campylobacter (c.) fetus is divided into the subspecies venerealis and fetus, which differ in epidemiology and clinical importance. the differences between these subspecies make an accurate distinction essential. differentiation of c. fetus by traditional microbiological methods is only based on two reactions (tolerance to glycin, na selenite reduction), in which c. fetus ssp. venerealis reacts negatively. however, the value of both reactions is limited. we used a specific pcr-based ... | 2003 | 12666499 |
| effect of two commercial vaccines to campylobacter fetus subspecies on heifers naturally challenged. | efficacy of two commercial vaccines containing campylobacter fetus subspecies on heifers naturally challenged by service with an infected bull was tested. sixteen heifers were vaccinated parentally two times with 3 weeks as interval, eight with commercial vaccine a and the other eight with commercial vaccine b. eight other heifers were used as unvaccinated controls. forty days after the first vaccine dose, the heifers were served by an infected bull during 60 days. measure of systemic immune res ... | 2003 | 12675898 |
| structure and genotypic plasticity of the campylobacter fetus sap locus. | the campylobacter fetus surface layer proteins (slps), encoded by five to nine sapa homologues, are major virulence factors. to characterize the sapa homologues further, a 65.9 kb c. fetus genomic region encompassing the sap locus from wild-type strain 23d was completely sequenced and analysed; 44 predicted open reading frames (orfs) were recognized. the 53.8 kb sap locus contained eight complete and one partial sapa homologues, varying from 2769 to 3879 bp, sharing conserved 553-2622 bp 5' regi ... | 2003 | 12694614 |
| failure of surface ring mutant strains of helicobacter mustelae to persistently infect the ferret stomach. | helicobacter mustelae, the gastric pathogen of ferrets, produces an array of surface ring structures which have not been described for any other member of the genus helicobacter, including h. pylori. the unique ring structures are composed of a protein named hsr. to investigate whether the hsr rings are important for colonization of the ferret stomach, ferrets specific pathogen free for h. mustelae were inoculated with an hsr-deficient mutant strain or the wild-type h. mustelae strain. quantitat ... | 2003 | 12704104 |
| humoral immune responses to s-layer-like proteins of bacteroides forsythus. | bacteroides forsythus is one of the important periodontopathic bacteria, and this microorganism is known to have an s-layer outside the outer membrane. the s-layer-like antigens were recently isolated from b. forsythus, and they were found to be 270- and 230-kda proteins in the envelope fraction. in this study, these proteins were confirmed to be specific to b. forsythus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and they were clearly recognized by sera from patients with adul ... | 2003 | 12738635 |
| campylobacter fetus bacteremia in a patient with adult t cell leukemia. | 2003 | 12766848 | |
| sandwich hybridisation assay for quantitative detection of yeast rnas in crude cell lysates. | background: a rapid microtiter plate based sandwich hybridization assay was developed for detection and quantification of single rna species using magnetic beads. following solution hybridization target rna molecules were collected by biotin-streptavidin affinity binding and detected by fluorescence signal generated by alkaline phosphatase. the 18s rrna and suc2 mrna of saccharomyces cerevisiae were used as model rna target molecules. results: the sensitivity of the assay was approximately 1.2 x ... | 2003 | 12780940 |
| phenotypic and molecular characterization of bovine campylobacter fetus strains isolated in brazil. | the objective of the present study was to characterize the phenotypic and molecular aspects of campylobacter fetus strains isolated from bovine herds with reproductive problems. thirty-one brazilian field isolates, together with one reference strain of each subspecies of c. fetus, were analyzed. the strains were submitted to phenotypic identification followed by subspecies characterization using the polymerase chain reaction (pcr) and numeric evaluation of restriction fragment length polymorphis ... | 2003 | 12637000 |
| [a man with campylobacter endocarditis, treatable as campylobacter fetus following identification]. | 2003 | 12892017 | |
| presumptive toxoplasma gondii abortion in a sheep. | a primiparous ewe aborted in mid-gestation. toxoplasma gondii was suspected as the cause of abortion and a presumptive diagnosis of t. gondii abortion was based on histological lesions of the placenta. | 2003 | 12715986 |
| simplified serological diagnosis of endocarditis due to coxiella burnetii and bartonella. | we tested a single-step serological assay against coxiella burnetii and bartonella species and found a sensitivity of 100%, and a positive predictive value of 98% for the diagnosis of blood culture-negative endocarditis (bcne). this assay should be considered as a possible commercial test for the diagnosis of bcne. | 2003 | 14607881 |
| heat resistance in liquids of enterococcus spp., listeria spp., escherichia coli, yersinia enterocolitica, salmonella spp. and campylobacter spp. | the aim of the work was to collect, evaluate, summarize and compare heat resistance data reported for campylobacter, enterococcus, escherichia, listeria, salmonella and yersinia spp. the work was limited to resistance in liquids with ph values 6-8. results obtained under similar experimental conditions were sought. thermal destruction lines for the various bacterial groups studied were constructed using log10 d values and treatment temperatures. there was a good linear relationship between log10 ... | 2003 | 14650540 |
| vaccines against sexually transmitted diseases. | 2003 | 14651753 | |
| effect of transport enrichment medium, transport time, and growth medium on the detection of campylobacter fetus subsp. venerealis. | the combination of medium and growth conditions, including transport enrichment medium (tem), transport time, tem incubation time, and growth medium, that best support campylobacter fetus subsp. venerealis while inhibiting contaminants was studied. the 3 tems evaluated, weybridge, cary-blair, and 0.85% saline solution, were inoculated with preputial smegma spiked with c. fetus subsp. venerealis and held in the laboratory for 4 or 24 hours before inoculation onto growth medium. the effect of over ... | 2002 | 12680641 |
| [a case of meningoencephalitis and spondylodiscitis caused by campylobacter fetus subsp. fetus infection]. | campylobacter fetus subsp. fetus (c. fetus) is a gram-negative, curved, rod-shaped microaerophile, occasionally may cause meningitis or meningoencephalitis in humans. this report documents the case of 49-year-old man with lumbar spondylodiscitis and meningoencephalitis caused by c. fetus infection. on admission, the patient was delirious and severe inflammatory reactions were seen in his serum. cerebrospinal fluid (cfs) revealed normal glucose concentration and moderate mononuclear leukocytosis. ... | 2002 | 12355851 |
| campylobacter-associated guillain-barré syndrome after orthotopic liver transplantation for hepatitis c cirrhosis: a case report. | guillain-barré syndrome is characterized by acute paralysis and ascending neuropathy due to an inflammatory attack on the myelin of peripheral nerves. about 2/3 of patients with guillain-barré syndrome have an infection 1-3 weeks before the onset of the symptoms. guillain-barré syndrome has rarely been reported after solid organ transplantation (18 cases with three cases after liver transplantation), and these cases are mostly related to a cmv infection. we describe a 64-year-old male patient wh ... | 2002 | 12270750 |
| structural analysis and evidence for dynamic emergence of bacillus anthracis s-layer networks. | surface layers (s-layers), which form the outermost layers of many bacteria and archaea, consist of protein molecules arranged in two-dimensional crystalline arrays. bacillus anthracis, a gram-positive, spore-forming bacterium, responsible for anthrax, synthesizes two abundant surface proteins: sap and ea1. regulatory studies showed that ea1 and sap appear sequentially at the surface of the parental strain. sap and ea1 can form arrays. the structural parameters of s-layers from mutant strains (e ... | 2002 | 12426331 |
| isolation of campylobacter fetus subsp. fetus from a patient with cellulitis. | campylobacter fetus subsp. fetus is a gram-negative, slender, spirally curved bacterial pathogen. it has been isolated from human blood, spinal fluid, and abscesses, but cellulitis associated with bacteremia is rare. we report its isolation from a blood culture of a human patient with cellulitis as well as difficulties encountered in determining the identity of the subspecies of c. fetus. | 2002 | 12454199 |
| thymoma associated with hypogammaglobulinemia (good's syndrome): report of a case. | a 63-year-old man was admitted to our hospital for treatment of hypogammaglobulinemia with thymoma (good's syndrome). tests for immunological function showed an abnormality in humoral immunity with decreases in the proportion of cells bearing b-cell markers in the peripheral blood and bone marrow. the patient was found to have campylobacter fetus sepsis caused by the hypogammaglobulinemia due to humoral immunodeficiency, and he was given gamma-globulin supplement. thymectomy was performed due to ... | 2002 | 11991514 |
| clostridium difficile genotyping based on slpa variable region in s-layer gene sequence: an alternative to serotyping. | recent investigations of clostridium difficile cell wall components have revealed the presence of an s-layer encoded by the slpa gene. the aim of this study was to determine whether slpa genotyping can be used as an alternative to serotyping. the variable regions of slpa were amplified by pcr from serogroup reference strains and various clinical isolates chosen randomly. amplified products were analyzed after restriction enzyme digestion and dna sequencing. the sequences of the variable region o ... | 2002 | 12089261 |
| effect of chlortetracycline in a trace mineral salt mix on fertility traits in beef cattle females in florida. | the study objective was to determine the effect of chlortetracycline in an ad libitum trace-mineralized salt mix given to heifers before and(or) during bull exposure on the proportion pregnant and the time to conception in a fixed breeding period. heifers (n = 768), 13 to 15 mo of age, were individually identified, immunized (leptospira, campylobacter fetus), examined (body condition score, vaginal lesion score, bw), and randomly allocated within 2 x 2 factorial blocks of treatments where trace ... | 2002 | 12002324 |
| development and evaluation of an enzyme-linked immunosorbent assay for quantification of the humoral response of cattle vaccinated against campylobacter fetus. | to develop a reliable elisa by use of a unique antigen preparation for serum igg quantification after vaccination against campylobacter fetus in cattle. | 2002 | 11939324 |
| patterns of sequence conservation in the s-layer proteins and related sequences in clostridium difficile. | clostridium difficile is the etiological agent of antibiotic-associated diarrhea. among the factors that may play a role in infection are s-layer proteins (slps). previous work has shown these to consist mainly of two components, resulting from the cleavage of a precursor encoded by the slpa gene. the high-molecular-weight (mw) subunit is related both to amidases from b. subtilis and to at least another 28 gene products in c. difficile strain 630. to gain insight into the functions of the slps a ... | 2002 | 12081960 |
| efficient isolation of campylobacters from stools: what are we missing? | 2002 | 11896083 | |
| campylobacter fetus bacteraemia in a renal graft recipient. | 2002 | 11917074 | |
| succinate:quinone oxidoreductases from epsilon-proteobacteria. | the epsilon-proteobacteria form a subdivision of the proteobacteria including the genera wolinella, campylobacter, helicobacter, sulfurospirillum, arcobacter and dehalospirillum. the majority of these bacteria are oxidase-positive microaerophiles indicating an electron transport chain with molecular oxygen as terminal electron acceptor. however, numerous members of the epsilon-proteobacteria also grow in the absence of oxygen. the common presence of menaquinone and fumarate reduction activity su ... | 2002 | 11803019 |
| monoclonal antibodies specific for campylobacter fetus lipopolysaccharides. | four monoclonal antibodies (mabs) (m1357, m1360, m1823 and m1825) which reacted with campylobacter fetus lipopolysaccharide (lps) core region epitopes were produced and characterized. reactivity of these mabs with c. fetus core lps epitopes was determined by enzyme-linked immunosorbent assay (elisa) with whole cell proteinase k digests and phenol-water extracted lps, and by immunoblotting with proteinase k digests. the specificities of the four mabs were evaluated using an indirect elisa. one of ... | 2002 | 12079745 |
| characterization of an autotrophic sulfide-oxidizing marine arcobacter sp. that produces filamentous sulfur. | a coastal marine sulfide-oxidizing autotrophic bacterium produces hydrophilic filamentous sulfur as a novel metabolic end product. phylogenetic analysis placed the organism in the genus arcobacter in the epsilon subdivision of the proteobacteria. this motile vibrioid organism can be considered difficult to grow, preferring to grow under microaerophilic conditions in flowing systems in which a sulfide-oxygen gradient has been established. purified cell cultures were maintained by using this appro ... | 2002 | 11772641 |
| campylobacter fetus adheres to and enters int 407 cells. | campylobacter fetus is a gram-negative bacterial pathogen of humans and ungulates and is normally transmitted via ingestion of contaminated food or water with infection resulting in mild to severe enteritis. however, despite clinical evidence that c. fetus infection often involves transient bacteremic states from which systemic infection may develop and the frequent isolation of c. fetus from extra-intestinal sites, this organism displays very poor invasiveness in in vitro models of infection. i ... | 2002 | 12556127 |
| long-term antibody responses following human infection with campylobacter jejuni. | epidemiological evidence suggests prior infection of humans by campylobacter jejuni leads to protection against disease following further exposure. it is known that infections elicit strong antibody responses following the onset of disease and that antibody levels are elevated in putatively immune populations. to determine if systemic and mucosal antibodies induced by a confirmed infection remain at elevated levels for prolonged periods, repeat serum, saliva and urine samples were taken from cam ... | 2002 | 12296859 |
| detection of helicobacter colonization of the murine lower bowel by genus-specific pcr-denaturing gradient gel electrophoresis. | helicobacter genus-specific pcr and denaturing gradient gel electrophoresis can detect and speciate the helicobacters that colonize the lower bowel of laboratory mice. the method's sensitivity is comparable to that of species-specific pcr and may detect unnamed helicobacter species. this approach should prove useful for commercial and research murine facilities. | 2002 | 12324369 |
| campylobacter-induced fetal death in a rhesus monkey. | a pregnant 4-year-old rhesus monkey (macaca mulatta) was presented with a history of acute vaginal bleeding. physical examination revealed an open cervix. an ultrasound scan demonstrated a viable early third-trimester fetus, approximately 16 weeks of gestational age. hematology results showed that the monkey was anemic, with a normal leukogram and döhle bodies. a subsequent cervical culture was positive for campylobacter fetus. the fetus died 3 days later, and a necropsy of the fetus and placent ... | 2002 | 12243477 |
| walkerton, 2 years later: "memory fades very quickly". | 2002 | 12041857 | |
| isolation of three new surface layer protein genes (slp) from lactobacillus brevis atcc 14869 and characterization of the change in their expression under aerated and anaerobic conditions. | two new surface layer (s-layer) proteins (slpb and slpd) were characterized, and three slp genes (slpb, slpc, and slpd) were isolated, sequenced, and studied for their expression in lactobacillus brevis neotype strain atcc 14869. under different growth conditions, l. brevis strain 14869 was found to form two colony types, smooth (s) and rough (r), and to express the s-layer proteins differently. under aerobic conditions r-colony type cells produced slpb and slpd proteins, whereas under anaerobic ... | 2002 | 12446628 |
| prevalence of campylobacter jejuni, campylobacter lari, and campylobacter coli in different ecological guilds and taxa of migrating birds. | a total of 1,794 migrating birds trapped at a coastal site in southern sweden were sampled for detection of campylobacter spp. all isolates phenotypically identified as campylobacter jejuni and a subset of those identified as non-c. jejuni were identified to the species level by pcr-based techniques. c. jejuni was found in 5.0% of the birds, campylobacter lari was found in 5.6%, and campylobacter coli was found in 0.9%. an additional 10.7% of the tested birds were infected with hippurate hydroly ... | 2002 | 12450810 |
| campylobacter fetus subspecies venerealis surface array protein from bovine isolates in brazil. | the electrophoretic patterns of 31 campylobacter fetus subspecies venerealis capsular surface array protein (sap) isolated from bovines in reproduction from different regions of brazil were analyzed. the persistence of the bacteria in the reproductive tract of naturally infected bovines and the dynamic of sap expression were also evaluated. cervical mucous and prepucial aspirates from five animals naturally infected were cultured for isolation of campylobacter fetus and the saps extracted from t ... | 2002 | 12070688 |
| thermo-tolerant campylobacter fetus bacteraemia identified by 16s ribosomal rna gene sequencing: an emerging pathogen in immunocompromised patients. | eight campylobacter isolates that were able to grow at 25 degrees c and 42 degrees c and had the same biochemical profile were isolated from the blood of eight immunocompromised patients. conventional biochemical tests were unable to determine whether they were isolates of thermo-tolerant c. fetus, h2s-negative c hyointestinalis, or a new campylobacter species. sequencing of the 16s ribosomal rna genes showed that all eight isolates had the same nucleotide sequence and this was identical to that ... | 2002 | 12358064 |
| evaluation of a novel heminested pcr assay based on the phosphoglucosamine mutase gene for detection of helicobacter pylori in saliva and dental plaque. | a novel heminested pcr protocol was developed for the specific detection of helicobacter pylori at low copy numbers. a set of primers specific for the phosphoglucosamine mutase gene (glmm) of h. pylori produced a 765-bp fragment that was used as template for the heminested primer pair delineating a 496-bp fragment. by using agarose gel electrophoresis for detection of the heminested pcr-amplified products, amplification of h. pylori genomic dna was achieved at concentrations as low as 0.1 pg, eq ... | 2002 | 11773117 |
| detection of campylobacter antibodies in sheep sera by a dot-elisa using acid extracts from c. fetus ssp. fetus and c. jejuni strains and comparison with a complement fixation test. | in this study, a dot-enzyme-linked immunosorbent assay (dot-elisa) was evaluated in comparison with a complement fixation test (cft) for the detection of campylobacter antibodies in sheep sera. acid glycine extracts (age) of both campylobacter fetus ssp. fetus and campylobacter jejuni strains that had been isolated from the gall-bladder of slaughtered sheep was used as antigen in both tests. a total of 153 sheep sera from aborted (74) and slaughtered (79) sheep were examined by both dot-elisa an ... | 2002 | 12019946 |
| development and application of a new scheme for typing campylobacter jejuni and campylobacter coli by pcr-based restriction fragment length polymorphism analysis. | a molecular typing approach for campylobacter jejuni and campylobacter coli was developed with restriction fragment length polymorphism analysis of a 9.6-kb pcr-amplified portion of the lipopolysaccharide gene cluster. sixty-one penner serotype reference strains were analyzed with this new genotyping scheme, and 32 genogroups were found. eleven additional genogroups were obtained from 87 clinical c. jejuni strains tested. this molecular typing method shows a correlation with the penner heat-stab ... | 2002 | 11980961 |
| growth of campylobacter jejuni supported by respiration of fumarate, nitrate, nitrite, trimethylamine-n-oxide, or dimethyl sulfoxide requires oxygen. | the human gastrointestinal pathogen campylobacter jejuni is a microaerophilic bacterium with a respiratory metabolism. the genome sequence of c. jejuni strain 11168 reveals the presence of genes that encode terminal reductases that are predicted to allow the use of a wide range of alternative electron acceptors to oxygen, including fumarate, nitrate, nitrite, and n- or s-oxides. all of these reductase activities were present in cells of strain 11168, and the molybdoenzyme encoded by cj0264c was ... | 2002 | 12107136 |
| recurrent septicemia due to campylobacter fetus and campylobacter lari in an immunocompetent patient. | we describe a severe and recurrent septicemia due to campylobacter in a 75-year-old immunocompetent patient. two campylobacter strains were detected in several blood cultures. campylobacter fetus and campylobacter lari were identified with pcr tests based on species-specific nucleotide sequences for the 16s rrna gene. | 2002 | 12120946 |
| homologs of the rml enzymes from salmonella enterica are responsible for dtdp-beta-l-rhamnose biosynthesis in the gram-positive thermophile aneurinibacillus thermoaerophilus dsm 10155. | the glycan chains of the surface layer (s-layer) glycoprotein from the gram-positive, thermophilic bacterium aneurinibacillus (formerly bacillus) thermoaerophilus strain dsm 10155 are composed of l-rhamnose- and d-glycero-d-manno-heptose-containing disaccharide repeating units which are linked to the s-layer polypeptide via core structures that have variable lengths and novel o-glycosidic linkages. in this work we investigated the enzymes involved in the biosynthesis of thymidine diphospho-l-rha ... | 2002 | 12147463 |
| fluorescent amplified fragment length polymorphism probabilistic database for identification of bacterial isolates from urinary tract infections. | the ability of the fluorescent amplified fragment length polymorphism (faflp) technique to identify bacterial isolates from urinary tract infections (utis) was investigated. faflp was carried out using the single primer combination msei plus ct and ecori plus 0, and information-rich faflp profiles were generated from all 69 uti isolates studied, which comprised both gram-negative and gram-positive bacteria encompassing eight genera. the genetic relatedness of these 69 bacteria was determined by ... | 2002 | 12149332 |
| campylobacter surface-layers (s-layers) and immune evasion. | many pathogenic bacteria have evolved mechanisms for evading host immune systems. one evasion mechanism is manifest by the surface layer (s-layer), a paracrystalline protein structure composed of s-layer proteins (slps). the s-layer, possessed by 2 campylobacter species (c. fetus and c. rectus), is external to the bacterial outer membrane and can have multiple functions in immune avoidance. c. fetus is a pathogen of ungulates and immunocompromised humans, in whom it causes disseminated bloodstre ... | 2002 | 16013216 |
| historical origins of current ibd concepts. | 2001 | 11819757 | |
| polarity in action: asymmetric protein localization in bacteria. | 2001 | 11344132 | |
| evaluation of numerical analysis of pfge-dna profiles for differentiating campylobacter fetus subspecies by comparison with phenotypic, pcr and 16s rdna sequencing methods. | to assess the efficacy of numerical analysis of pfge-dna profiles for identification and differentiation of campylobacter fetus subspecies. | 2001 | 11168732 |
| thermophilic multidrug-resistant campylobacter fetus infection with hypersplenism and histiocytic phagocytosis in a patient with acquired immunodeficiency syndrome. | we present a case report of a patient who had acquired immunodeficiency syndrome (aids) and campylobacter fetus infection with a number of unusual clinical and microbiological features. the patient had prominent gastrointestinal symptoms, splenic infarction, splenomegaly with hypersplenism, and hemophagocytic histiocytosis in the spleen and lymph nodes; the organism displayed growth on campy-selective blood agar, thermotolerance, and resistance to quinolones, piperacillin/tazobactam, ceftazidime ... | 2001 | 11170921 |
| n-acyl-l-homoserine lactone-mediated regulation of the lip secretion system in serratia liquefaciens mg1. | the analysis of serratia liquefaciens mg1 'luxab insertion mutants that are responsive to n-butanoyl-l-homoserine lactone revealed that expression of lipb is controlled by the swr quorum-sensing system. lipb is part of the lip exporter, a type i secretion system, which is responsible for the secretion of extracellular lipase, metalloprotease, and s-layer protein. | 2001 | 11160117 |
| evidence that the campylobacter fetus sap locus is an ancient genomic constituent with origins before mammals and reptiles diverged. | campylobacter fetus bacteria, isolated from both mammals and reptiles, may be either subsp. fetus or subsp. venerealis and either serotype a or serotype b. surface layer proteins, expressed and secreted by genes in the sap locus, play an important role in c. fetus virulence. to assess whether the sap locus represents a pathogenicity island and to gain further insights into c. fetus evolution, we examined several c. fetus genes in 18 isolates. all of the isolates had 5 to 9 sapa or sapb homologs. ... | 2001 | 11254579 |
| campylobacter fetus subspecies fetus bacteremia associated with chorioamnionitis and intact fetal membranes. | campylobacter fetus subspecies fetus was isolated from the blood of a patient with chorioamnionitis and intact fetal membranes. the mother improved after appropriate antibiotic treatment, but the infant died of neonatal infection a few minutes after delivery. this is a very unusual etiology of intra-amniotic infection. | 2001 | 11233847 |
| escherichia coli cdtb mediates cytolethal distending toxin cell cycle arrest. | we previously reported that the cdtb polypeptide of escherichia coli cytolethal distending toxin (cdt) shares significant pattern-specific homology with mammalian type i dnases. in addition, the dnase-related residues of cdtb are required for cellular toxicity. here we demonstrate that purified cdtb converts supercoiled plasmid dna to relaxed and linear forms and promotes cell cycle arrest when combined with an e. coli extract containing cdta and cdtc. cdtb alone had no effect on hela cells, how ... | 2001 | 11292766 |
| [a case of infected subdural hematoma due to campyrobacter fetus]. | a 20-year-old male presented fever, nausea and headache 1 week after eating uncooked liver. on admission, it was revealed he had suffered a closed head injury 3 months before without unconsciousness. on admission, computed tomography scan showed a left chronic subdural hematoma. gd-dtpa magnetic resonance images revealed unusual enhancement of the capsule and linear enhancement of the subarachnoid space. the patient underwent burr hole irrigation and drainage. the culture of the hematoma content ... | 2001 | 11321797 |
| [pleural empyema and pericardial effusion due to campylobacter fetus in an immunocompetent adult]. | 2001 | 11412511 | |
| juxtaposition of an active promoter to vsp genes via site-specific dna inversions generates antigenic variation in mycoplasma bovis. | mycoplasma bovis, the most important etiological agent of bovine mycoplasmosis, undergoes extensive antigenic variation of major and highly immunogenic surface lipoprotein antigens (vsps). a family of 13 related but divergent vsp genes, which occur as single chromosomal copies, was recently found in the chromosome of m. bovis. in the present study, the molecular mechanism mediating the high-frequency phase variation of two vsps (vspa and vspc) as representatives of the vsp family was investigate ... | 2001 | 11544233 |
| locus of enterocyte effacement from citrobacter rodentium: sequence analysis and evidence for horizontal transfer among attaching and effacing pathogens. | the family of attaching and effacing (a/e) bacterial pathogens, which includes diarrheagenic enteropathogenic escherichia coli (epec) and enterohemorrhagic e. coli (ehec), remains a significant threat to human and animal health. these bacteria intimately attach to host intestinal cells, causing the effacement of brush border microvilli. the genes responsible for this phenotype are encoded in a pathogenicity island called the locus of enterocyte effacement (lee). citrobacter rodentium is the only ... | 2001 | 11553577 |
| campylobacter fetus diagnosis: direct immunofluorescence comparing chicken igy and rabbit igg conjugates. | in argentina bovine genital campylobacteriosis is routinely diagnosed by direct immunofluorescence test. generally, the hyperimmune sera used for this test are obtained from rabbits and less often from goats. in this work, a chicken egg yolk immunoglobulin (igy) extract was conjugated and its ability to detect campylobacters with the regular conjugate prepared with rabbit sera was comparatively evaluated. both conjugates were independently evaluated by two laboratories, named "azul" (lab a) and ... | 2001 | 11565050 |
| campylobacter fetus uses multiple loci for dna inversion within the 5' conserved regions of sap homologs. | campylobacter fetus cells possess multiple promoterless sap homologs, each capable of expressing a surface layer protein (slp) by utilizing a unique promoter present on a 6.2-kb invertible element. each sap homolog includes a 626-bp 5' conserved region (fcr) with 74 bp upstream and 552 bp within the open reading frame. after dna inversion, the splice is seamless because the fcrs are identical. in mutant strain 23d:aca2k101, in which sapa and sapa2 flanking the invertible element in opposite orie ... | 2001 | 11673436 |
| s-layer variation in bacillus stearothermophilus pv72 is based on dna rearrangements between the chromosome and the naturally occurring megaplasmids. | bacillus stearothermophilus pv72 expresses different s-layer genes (sbsa and sbsb) under different growth conditions. no stretches of significant sequence identity between sbsa and sbsb were detected. in order to investigate s-layer gene regulation in b. stearothermophilus pv72, we characterized the upstream regulatory region of sbsa and sbsb by sequencing and primer extension analysis. both genes are transcribed from unique but different promoters, independently of the growth phase. localizatio ... | 2001 | 11160098 |
| characterization of culture-derived spiral bacteria by 16s ribosomal rna gene sequence analysis. | broad range amplification and sequence analysis of the 16s ribosomal rna gene was used to identify three spiral-form organisms. the agents were identified as campylobacter fetus, "flexispira rappini", and borrelia burgdorferi, respectively, using either proprietary or public sequence databases. in each case, the rdna sequence showed 99-100% homology with known sequence data. sequence-based analysis for each isolate required only 2-3 days, whereas traditional means of identification took 8-12 day ... | 2001 | 11173192 |
| evidence for a genetically stable strain of campylobacter jejuni. | the genetic stability of selected epidemiologically linked strains of campylobacter jejuni during outbreak situations was investigated by using subtyping techniques. strains isolated from geographically related chicken flock outbreaks in 1998 and from a human outbreak in 1981 were investigated. there was little similarity in the strains obtained from the different chicken flock outbreaks; however, the strains from each of three chicken outbreaks, including strains isolated from various environme ... | 2001 | 11229909 |
| determination of helicobacter pylori virulence by simple gene analysis of the cag pathogenicity island. | nucleic acid amplification was performed for five loci in the cag pathogenicity island (pai) of helicobacter pylori (comprising caga, the caga promoter region, cage, cagt, and the left end of cagii [lec]), and gastric inflammation in patients was evaluated. of 204 h. pylori isolates from japanese patients (53 with peptic ulcer, 55 with gastric cancer, and 96 with chronic gastritis), 197 (96.6%) were positive for all five loci. two isolates (1%) were negative for all five loci, and five isolates ... | 2001 | 11139216 |
| comparison of genotypes and serotypes of campylobacter jejuni isolated from danish wild mammals and birds and from broiler flocks and humans. | the incidence of human infection with campylobacter jejuni is increasing in most developed countries and the reason for this is largely unknown. although poultry meat is considered to be a major source, it is evident that other reservoirs exist, possibly common to humans and poultry. environmental sources are believed to be important reservoirs of campylobacter infection in broiler chicken flocks. we investigated the potential importance of wildlife as a source of infection in commercial poultry ... | 2001 | 11425730 |
| evaluation of methods for subtyping campylobacter jejuni during an outbreak involving a food handler. | in october 1998, the centers for disease control and prevention (cdc) assisted in an investigation of an outbreak of campylobacteriosis at a school in salina, kansas. twenty-two isolates were submitted from the kansas state public health laboratory to cdc, 9 associated with the outbreak and 13 epidemiologically unrelated sporadic isolates. pulsed-field gel electrophoresis (pfge) using smai and sali was initially used to validate the epidemiologic data. we then tested the ability of other subtypi ... | 2001 | 11427543 |
| helicobacter winghamensis sp. nov., a novel helicobacter sp. isolated from patients with gastroenteritis. | from 1997 to 1999 seven isolates of campylobacter-like organisms from five patients that were exhibiting symptoms of gastroenteritis, including fever, stomach malaise, and diarrhea, were investigated. the organisms were isolated from stool samples and found to exhibit a diverse colony morphology; hence multiple isolates were submitted from one of the patients. all isolates were found to be identical. the organisms were catalase, urease, alkaline phosphatase, and nitrate negative but oxidase and ... | 2001 | 11427547 |
| genomic heterogeneity and o-antigenic diversity of campylobacter upsaliensis and campylobacter helveticus strains isolated from dogs and cats in germany. | a serotyping scheme based on heat-stable surface antigens was established for 101 campylobacter upsaliensis and 10 campylobacter helveticus strains isolated from 261 dogs and 46 cats of different ages originating from two geographically distinct regions in germany. the prevalence of c. upsaliensis varied between 27.8% in juvenile dogs (<12 months of age) and 55.4% in adult dogs (p < 0.05). of the cats, 19.6% harbored c. upsaliensis, whereas 21.7% carried c. helveticus. of the c. upsaliensis isol ... | 2001 | 11427567 |
| cytolethal distending toxin demonstrates genotoxic activity in a yeast model. | cytolethal distending toxins (cdts) are multisubunit proteins produced by a variety of bacterial pathogens that cause enlargement, cell cycle arrest, and apoptosis in mammalian cells. while their function remains uncertain, recent studies suggest that they can act as intracellular dnases in mammalian cells. here we establish a novel yeast model for understanding cdt-associated disease. expression of the cdtb subunit in yeast causes a g2/m arrest, as seen in mammalian cells. cdtb toxicity is not ... | 2001 | 11500452 |
| prevalence, antigenic specificity, and bactericidal activity of poultry anti-campylobacter maternal antibodies. | poultry are considered the major reservoir for campylobacter jejuni, a leading bacterial cause of human food-borne diarrhea. to understand the ecology of c. jejuni and develop strategies to control c. jejuni infection in the animal reservoir, we initiated studies to examine the potential role of anti-campylobacter maternal antibodies in protecting young broiler chickens from infection by c. jejuni. using an enzyme-linked immunosorbent assay (elisa), the prevalence of anti-c. jejuni antibodies in ... | 2001 | 11525990 |
| survival of clinical and poultry-derived isolates of campylobacter jejuni at a low temperature (4 degrees c). | campylobacter jejuni is a leading cause of bacterial gastroenteritis in humans, and contamination of poultry has been implicated in illness. the bacteria are fastidious in terms of their temperature requirements, being unable to grow below ca. 31 degrees c, but have been found to be physiologically active at lower temperatures and to tolerate exposure to low temperatures in a strain-dependent manner. in this study, 19 field isolates of c. jejuni (10 of clinical and 9 of poultry origin) were stud ... | 2001 | 11526022 |
| phenotyping of campylobacter jejuni and campylobacter coli by a quantitative antibiogram [mic] typing scheme using euclidean distances [qated]. | background: enteropathogenic campylobacter jejuni and c. coli are presently the most common cause of acute bacterial gastroenteritis in the developed world. an understanding of sources and means of transmission of campylobacter is an essential factor in order to reduce the incidence of campylobacter-related gastroenteritis in man. consequently a reproducible, sensitive and well-standardised typing scheme is critical in the successful discrimination of strains and in the subsequent investigations ... | 2001 | 11527505 |
| polar flagellar motility of the vibrionaceae. | polar flagella of vibrio species can rotate at speeds as high as 100,000 rpm and effectively propel the bacteria in liquid as fast as 60 microm/s. the sodium motive force powers rotation of the filament, which acts as a propeller. the filament is complex, composed of multiple subunits, and sheathed by an extension of the cell outer membrane. the regulatory circuitry controlling expression of the polar flagellar genes of members of the vibrionaceae is different from the peritrichous system of ent ... | 2001 | 11528005 |
| rapid pulsed-field gel electrophoresis protocol for subtyping of campylobacter jejuni. | we developed a rapid pulsed-field gel electrophoresis (pfge) protocol for subtyping campylobacter isolates based on the standardized protocols used by pulsenet laboratories for the subtyping of other food-borne bacterial pathogens. various combinations of buffers, reagents, reaction conditions (e.g., cell suspension concentration, lysis time, lysis temperature, and restriction enzyme concentration), and electrophoretic parameters were evaluated in an effort to devise a protocol that is simple, r ... | 2001 | 11326009 |
| comparison of two methods for serotyping campylobacter spp. | two serotyping schemes (penner and laboratory of enteric pathogens [lep]) based on soluble heat-stable antigens were used to analyze 3,788 campylobacter sp. isolates. a significant percentage (36.6%) was untypeable using lep serotyping; greater cross-reaction was also observed. the relative discrimination capabilities of the techniques were similar. penner serotyping fulfils more of the requisite criteria for typing methods. | 2001 | 11326013 |
| [femoral aneurysm infected by campylobacter fetus]. | 2001 | 11333725 | |
| intrachromosomal recombination within the vsp locus of mycoplasma bovis generates a chimeric variable surface lipoprotein antigen. | a family of 13 related but divergent vsp genes was recently found in the chromosome of the bovine pathogen mycoplasma bovis. the vsp genomic locus was shown to undergo high-frequency rearrangements and to mediate phenotypic switching of variable lipoprotein antigens (vsps) on the mycoplasma cell surface. here we report that the vsp gene repertoire is subject to changes. genetic analysis of m. bovis clonal isolates displaying distinct vsp phenotypes showed that an intergenic recombination event b ... | 2001 | 11349034 |