Publications

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[vibrio fetus septicemia (review of the literature apropos of a case of endocarditis)]. 19685748195
[form changes of o-antigens in vibrio fetus]. 19685750827
the serological and bacteriological diagnosis of vibrio fetus infection in indian cattle and buffaloes. 19685752784
vibrio fetus septicemia. 19695779404
the occurrence of vibrio fetus (intestinalis) in australian cattle. 19695814098
observations on the isolation of vibrio fetus (venerealis) from the vaginal mucus of experimentally infected heifers. 19695815409
the relative incidence of vibrio fetus infection in dairy and beef cattle in new south wales. 19695815887
protective antigens of vibrio fetus in sheep. 19695816363
vibrio fetus var. intestinalis isolated from the intestinal content of birds. 19695816369
[one case of vibrio fetus in rattus norvegicus]. 19695817691
characterization of the antibody for vibrio fetus endotoxin in sera of normal and v. fetus infected cattle. 19655850536
[experiments on the electron microscopic differentiation of vibrio fetus, vibrio bubulus, vibrio coli and vibrio metschnikovi]. 19655881830
pathologic responses in animals after vibrio fetus toxin shock. 19655895112
[disinfection of bull sperm from vibrio fetus]. 19655896158
vibrio fetus endocarditis. report of 2 cases. 19665905454
antral mucosal diaphragm. clinical and roentgen characteristics, with first reported case of vibrio fetus in human bile. 19665930786
pericarditis due to vibrio fetus. 19665949068
an antigenic analysis of vibrio fetus. 3. chemical, biologic, and antigenic properties of the endotoxin. 19665961409
anatomical features of vibrio fetus: electron microscopic survey. 19665963508
the preparation and characterization of cell walls and the preparation of flagella of vibrio fetus. 19665963509
[vibrio fetus causing meningitis in an infant]. 19665984727
[studies on strains of vibrio fetus isolated from humans]. 19665985326
[a comparative study of the sensitivity of vibrio fetus to antibiotics]. 19665988723
histopathology of bovine vibriosis and the effects of vibrio fetus extracts on the female genital tract. 19666008348
the effect of vibrio fetus vaccination on the breeding efficiency of cows bred to vibrio fetus-infected bulls. 19676053535
infection caused by vibrio fetus. report of two cases. 19676065952
experimental vibrio fetus infection in cows. studies on the immunising properties of living organisms injected subcutaneously. 19676069454
diagnosis of bovine vibriosis. 1. the production and use of standard suspensions of vibrio fetus agglutinating antigen. 19676069698
restriction endonuclease analysis of campylobacter strains with particular reference to campylobacter fetus ss. fetus.forty-three strains of campylobacter fetus ss. fetus isolated from sheep abortions in new zealand, and reference strains of c. fetus ss. fetus (four), c. fetus ss. venerealis (two), c. jejuni (one) and c. coli (one) were examined by restriction endonuclease analysis with the enzymes bste ii and xho i. dna fragment patterns of c. fetus, c. jejuni and c. coli differed strikingly from each other, but there were many similarities in the patterns obtained for all strains of c. fetus ss. fetus (47) an ...19846086928
three-year prospective study of intestinal pathogens in madrid, spain.during the period july 1980 through june 1983, in a general hospital in madrid, the following organisms were detected from 6,970 patients with gastroenteritis: 710 salmonella spp. 506 campylobacter jejuni, 379 shigella spp., 12 yersinia enterocolitica, 1,466 rotavirus, 134 giardia lamblia, and 4 entamoeba histolytica. chloramphenicol showed good activity against most tested strains of salmonella spp., shigella spp., and c. jejuni. the incidence of salmonella spp. and shigella spp. was very marke ...19846092418
plasma alpha-fetoprotein concentrations in pregnant cows exposed to sarcocystis cruzi, campylobacter fetus, or aspergillus fumigatus.bovine alpha-fetoprotein (afp) was determined in maternal plasma, using radioimmunoassay in an attempt to detect and monitor fetal distress in pregnant cows. plasma from pregnant cows in the 4th to 5th month of the gestation which had been exposed to sarcocystis cruzi, campylobacter fetus, or aspergillus fumigatus was used. plasma afp concentrations were determined at intervals from before the cows were exposed until they had aborted or calved. the plasma afp concentration of the exposed pregnan ...19816176152
growth of non-campylobacter, oxidase-positive bacteria on selective campylobacter agar.a total of 67 oxidase-positive, gram-negative bacteria were tested for growth on selective campylobacter agar (blaser formulation, bbl microbiology systems, cockeysville, md.) at 42 degrees c under microaerophilic conditions. although the growth of most of these bacteria was prevented, all strains of achromobacter xylosoxidans, pseudomonas aeruginosa, pseudomonas putrefaciens, pseudomonas alcaligenes, and pseudomonas pseudoalcaligenes grew as well as campylobacter fetus subsp. jejuni.19826179961
campylobacteriosis--a "new" disease in laboratory animals.campylobacter fetus ss jejuni is now recognized as a leading cause of diarrhea in humans. with the development of proper culture techniques, c jejuni has been isolated from humans with diarrhea as frequently as salmonella or shigella species. laboratory animal models are being developed to study the pathogenesis of campylobacter-induced diarrhea; also, identification of c jejuni is occurring more frequently in a variety of laboratory animals, whether asymptomatic carriers or those with clinical ...19826186839
bovine genital campylobacteriosis (vibriosis): vaccination of experimentally infected bulls.the therapeutic efficacy of a campylobacter fetus subsp venerealis bacterin was determined in experimentally infected bulls. ten of twelve 5-year-old angus bulls became infected after being infused intrapreputially with c fetus subsp venerealis. of the 10 bulls, 6 were vaccinated with 5 ml of c fetus subsp venerealis vaccine on 2 occasions 4 weeks apart. preputial washings of the vaccinated bulls were culturally negative by the 8th week after primary vaccination. none of the 18 heifers exposed t ...19836194723
nucleic acids in the classification of campylobacters.the importance of campylobacters in human disease has stimulated improvements in the methods for identification of strains from hospitals and the environment. reliable and accurate identification depends on a sound classification for which nucleic acid analyses provide fundamental information about species relationships. studies on the genus campylobacter show that the genome dna of species have base compositions of 29 to 38 mol% g + c and molecular weights of 1.54 x 10(9) to 2.31 x 10(9). campy ...19836194994
human antibody response to outer membrane proteins of campylobacter jejuni during infection.two techniques were used to isolate outer membrane proteins from campylobacter jejuni, edta-lysozyme extraction and sodium-n-lauroylsarcosinate (sarkosyl) solubilization. the protein profiles of the two preparations were similar, with a few additional bands in the edta-lysozyme preparations. the major outer membrane protein was 43,000 (43k) daltons, and there were 8 to 10 minor bands ranging from 92k to 14k daltons. there was no difference in the protein profile of a strain causing an infection ...19846198286
[enterocolitis caused by campylobacter fetus subsp. jejuni--epidemiology and hygiene with special reference to federal legislation and public health measures]. 19826213894
in vitro antibacterial activity of norfloxacin (mk-0366, am-715) and other agents against gastrointestinal tract pathogens.a comparison was made of the in vitro activities of norfloxacin and of nine other orally administered antibacterial agents against 180 clinical isolates representing the bacterial species most frequently implicated in infections of the gastrointestinal tract in humans. the 90% minimal inhibitory concentrations showed norfloxacin to be 4, 15, 4, 17, 17, 17, and 33 times more active than the next best compound tested against campylobacter fetus subsp. jejuni, escherichia coli, salmonella spp., shi ...19836219622
[campylobacter fetus ssp fetus thrombophlebitis]. 19846229730
[bacteriocinogenicity of brucellae isolated in foci in the caucasus and their evaluation from taxonomic viewpoints].the aim of the study was to elucidate the possibility of using bacteriocinogenicity of brucella as taxonomic feature, to determine their phylogenetic relation to other microorganisms by their bacteriocinogenic properties and to investigate the physicochemical properties of brucellacin and conditions for its stable detection. the brucella cultures were isolated in the caucasus. investigation of their capacity for production of bacteriocin according to the procedure described by m.a. konstantinova ...19846230043
immune-mediated migration of neutrophils into the uterine lumen of guinea pigs.metritis was elicited by intrauterine infusion of tuberculin or killed campylobacter fetus ssp. venerealis into vaccinated guinea pigs and lipopolysaccharide or immune complexes into normal animals. the local inflammatory response to intrauterine infusion of antigens, lipopolysaccharide, and immune complexes was determined by changes in differential cell counts in the uterine lavage fluid and by histopathological examination of uterine tissue. the percentage of neutrophils was significantly (p l ...19846241431
respiratory systems and cytochromes in campylobacter fetus subsp. intestinalis.cell suspensions of campylobacter fetus subsp. intestinalis grown microaerophilically in complex media consumed oxygen in the presence of formate, succinate, and dl-lactate, and membranes had the corresponding dehydrogenase activities. the cells and membranes also had ascorbate-n,n,n',n'-tetramethyl-p-phenylenediamine oxidase activity which was cyanide sensitive. the fumarate reductase activity in the membranes was inhibited by p-chloromercuriphenylsulfonate, and this enzyme was probably respons ...19806254951
[a case report of septicaemia due to campylobacter fetus variety intestinalis on a cancerous patient (author's transl)].the authors report a case of campylobacter fetus septicaemia from a cancerous patient. the bacterium was isolated from three blood cultures. the infection characteristics are briefly discussed.19806256917
summer diarrhoea in african infants and children.of 70 black south african infants and children with acute summer diarrhoea, 30 (43%) were infected with enteropathogenic serogroups of escherichia coli (epec), 13 (19%) with enterotoxigenic gram-negative bacilli, 12 (17%) with salmonella sp., 6 (9%) with shigella sp., and 3 (4%) with rotaviruses. 13 (19%) patients were infected simultaneously with more than one enteropathogen, and no pathogen was detected in 22 (31%). in addition, 6 (15%) of 41 unselected patients were excreting campylobacter fe ...19806257185
bactericidal activity of antibiotics against campylobacter fetus subspecies intestinalis. 19816262415
travelers' diarrhea in panamanian tourists in mexico.to determine whether residents of developing countries are unlikely to acquire travelers' diarrhea, 64 panamanians of widely divergent socioeconomic strata were studied during a 15-day tour through mexico. twenty-three (36%) tourists experienced 27 episodes of travelers' diarrhea that were caused by seven different pathogens. the most commonly identified etiologic agents were rotavirus (26%), norwalk virus (15%), and campylobacter fetus (11%), whereas enterotoxigenic escherichia coli was not fre ...19816273474
the relative importance of rotavirus and other pathogens in the etiology of gastroenteritis in trinidadian children.over a 2-year period, 300 infants less than 3 years old with gastroenteritis admitted to hospitals in trinidad were investigated for the presence of certain microorganisms in the feces, along with an equal number of age- and sex-matched controls. rotavirus was detected in 23% of cases and 1% of controls; salmonella in 7% of cases and in 1% of controls; shigella in 4% of cases and in no controls and two serotypes of enteropathogenic e. coli in 7% of cases and in 2% of controls. campylobacter fetu ...19826277207
respiratory physiology and energy conservation efficiency of campylobacter jejuni.a study of the electron transport chain of the human intestinal pathogen campylobacter jejuni revealed a rich complement of b- and c-type cytochromes. two c-type cytochromes were partially purified: one, possibly an oxidase, bound carbon monoxide whereas the other, of high potential was unreactive with carbon monoxide. respiratory activities determined with membrane vesicles were 50- to 100-fold higher with formate and hydrogen than with succinate, lactate, malate, or nadh as substrates. evidenc ...19826277867
aerobic and anaerobic respiratory systems in campylobacter fetus subsp. jejuni grown in atmospheres containing hydrogen.maximum growth of campylobacter fetus subsp. jejuni, strain c-61, occurred when the cultures were incubated with shaking in atmospheres containing approximately 30% hydrogen, 5% oxygen, and 10% co2. suspensions of cells grown under these conditions consumed oxygen with formate as the substrate in the presence of 0.33 mm cyanide, which completely inhibited respiration with ascorbate-n,n,n',n'-tetramethyl-p-phenylenediamine and with lactate. spectroscopic evidence with intact cells suggested that ...19826288661
effects of disinfectants on campylobacter jejuni.because of the increasing recognition that campylobacter jejuni is an important enteric pathogen of humans, we studied the effects of widely used disinfectants on the viability of this organism. at an inoculum size of 10(3) to 10(4) cfu/ml, 1.25 mg of hypochlorite per liter killed three strains within 1 min. at an inoculum size of 10(6) to 10(7) cfu/ml, 5 mg of hypochlorite per liter killed three strains within 15 min. killing of similar concentrations of c. jejuni and escherichia coli by hypoch ...19836305266
value of stool examination in patients with diarrhoea.findings of stool examinations in 1593 patients with diarrhoea due to a single enteric pathogen--enterotoxigenic escherichia coli rotavirus, shigella, campylobacter jejuni, vibrio cholerae 0:1, entamoeba histolytica, or giardia lamblia--were reviewed to determine how well they predicted the agent associated with the diarrhoea. specimens were examined visually for blood and mucus, tested for ph, and examined under a microscope for the presence of red and white blood cells, parasites, and stool fa ...19836307460
the polymicrobial origin of intestinal infections in homosexual men.to determine the microbial cause and the clinical and pathologic correlates of anorectal and intestinal symptoms in homosexually active men, we performed comprehensive microbiologic studies, anoscopy, sigmoid-oscopy, and rectal biopsy in men examined in a clinic for sexually transmitted diseases. enteric pathogens were found in 95 of 119 consecutive homosexual men with anorectal or intestinal symptoms and in 29 of 75 randomly selected homosexual men without such symptoms (p less than 0.001). the ...19836308444
application of serotyping and chromosomal restriction endonuclease digest analysis in investigating a laboratory-acquired case of campylobacter jejuni enteritis.a frequently passaged laboratory strain of campylobacter jejuni was confirmed by serotyping on the basis of thermostable antigens and by bacterial chromosomal restriction endonuclease digests to be the causative agent of enteritis in a laboratory worker.19836317712
characterization of tetracycline resistance plasmids from campylobacter jejuni and campylobacter coli.tetracycline resistance in strains of campylobacter jejuni and campylobacter coli was mediated by plasmids. intra- and interspecies transfer was demonstrated within the genus campylobacter. buoyant densities of plasmid dnas ranged from 1.691 to 1.694 g/cm3 (31 to 33% guanine plus cytosine). restriction enzymes acci, bcli, bglii, and psti were found to be most useful for comparing the plasmids. the molecular weight of c. jejuni plasmid pmak175 was 44.7 kilobases (29 x 10(6), and the other plasmid ...19836318666
[campylobacter fetus fetus bacteremia. review of the literature apropos of 2 cases].the authors report two cases of bacteremia due to campylobacter fetus fetus (also called c. fetus intestinalis). one was a 22-year-old female, under treatment for active systemic lupus erythematosus, who died. the other was a fifty-year-old male, in a poor general condition, who had a self-limited acute febrile dysentery. in this patient, c. fetus fetus was found in stools after apparent recovery, raising the problem of healthy carriers. a review of the literature shows 64 other such cases.19836320419
investigation of a campylobacter jejuni outbreak by serotyping and chromosomal restriction endonuclease analysis.fifty campylobacter jejuni isolates, including 29 from humans associated with an outbreak of enteritis, 20 from cattle, and 1 from a milk source, were serotyped on the basis of extractable thermostable antigens and examined by bacterial chromosomal restriction endonuclease digest analysis. serotyping showed specific differences between the human isolates and the milk isolates, but each of these generally, although not consistently, reacted with 4 of the 42 c. jejuni typing antisera. restriction ...19846325487
parasitic, bacterial, and viral enteric pathogens associated with diarrhea in the central african republic.a total of 1,197 diarrheic children less than 15 years old were investigated for parasitic, bacterial, and viral enteropathogens from march 1981 through february 1982 in the central african republic. one or more pathogens were identified from 49.4% of the patients. rotavirus was the most frequently identified pathogen among children less than 18 months old. enteropathogenic escherichia coli was the second most frequently isolated pathogen (12.1%) in children less than 2 years of age. campylobact ...19846330161
diarrhea associated with rotaviruses, enterotoxigenic escherichia coli, campylobacter, and other agents in costa rican children, 1976-1981.rotaviruses, enterotoxigenic escherichia coli, salmonella, shigella, and parasites were investigated in outpatient diarrheic children, and in hospitalized diarrheic and non-diarrheic children, between january 1976 and june 1979. in outpatient cases studied within 4 days of onset of symptoms, rotaviruses were the most common agents (45.3%); e. coli heat-stable enterotoxin ranked second (13.4%); shigella was third (8.1%); salmonella was fourth (7.3%). in 63.2%, one or more enteric agents were dete ...19836337517
avian wildlife reservoir of campylobacter fetus subsp. jejuni, yersinia spp., and salmonella spp. in norway.cloacal swabs from 540 wild-living birds were cultured for campylobacter fetus subsp. jejuni, yersinia spp., and salmonella spp. the carrier rates detected were as follows: c. fetus subsp. jejuni, 28.4%; yersinia spp., 1.2%; and salmonella spp., 0.8%. all birds were apparently healthy when captured. c. fetus subsp. jejuni was isolated from 11 of the 40 bird species examined. among birds inhabiting the city of oslo, the highest isolation rate was found in crows (corvus corone cornix) (89.8%), fol ...19836338824
serogroups of campylobacter jejuni, campylobacter coli, and campylobacter fetus defined by direct immunofluorescence.rabbits were inoculated with whole, formalinized campylobacter jejuni, c. coli, and c. fetus cells; the c. jejuni and c. coli immunogens were identified by their dna relatedness at the species level to the type strains of c. jejuni or c. coli. the designation c. coli was not used among the other c. jejuni strains; they were classified as hippurate-positive or hippurate-negative c. jejuni. immunoglobulin g was isolated from the antisera and labeled with fluorescein isothiocyanate. these conjugate ...19836341405
an acid extract as a common antigen in campylobacter coli and campylobacter jejuni strains.an acid extract prepared from a single strain of campylobacter jejuni reacted with rabbit antisera against 21 strains of c. jejuni and campylobacter coli. the immunogen is resistant to formalin and to exposure to 100 degrees c for 30 min. the antigenic extract may have practical applications in human serology.19836343426
[campylobacter infection in children].enteritis by campylobacter jejuni/coli is by far the most frequent clinical entity of campylobacter-infection. it may be compared to salmonella-enteritis as for its epidemiologic importance. the course of the disease may be dramatic, but can be successfully treated as soon as the pathogen is known. in prematures and newborn babies infections with campylobacter fetus ssp. fetus are life-threatening and early diagnosis is of life-saving importance. five observations of campylobacter-enteritis in c ...19836352463
isolation of campylobacter jejuni from an appendix.campylobacter jejuni skirrow biotype 1, lior serotype 8 was isolated from the appendix of an 11-year-old boy who had a 6-h history of acute abdominal pain. histological diagnosis on the appendix section was early acute appendicitis. dilute carbol fuchsin stain and indirect fluorescent antibody test performed on the appendix section also revealed the presence of campylobacter sp. the patient developed a significant bactericidal antibody titer of 1,024, providing substantial clinical evidence of t ...19836352736
evaluation of transport media for campylobacter jejuni in human fecal specimens.it is not always possible to culture feces immediately, and appropriate methods for transport of human specimens, unlike those from animals, have not been fully evaluated. therefore, we took serial subcultures in two phases from six transport media inoculated with human diarrheal stools known to be positive for campylobacter jejuni. in phase 1, cary-blair medium and buffered glycerol saline did not preserve c. jejuni as well as did alkaline peptone-water (apw), modified cary-blair medium, thiogl ...19836355160
detection of campylobacter fetus in artificial insemination bulls with a transport enrichment medium.one hundred and five bulls from an artificial insemination unit were tested for the presence of campylobacter fetus subspecies venerealis. the method involved the inoculation of preputial samples into a new transport enrichment medium prior to culture and immunofluorescence tests. seventeen bulls (16%) were found to be either positive or suspected carriers of c. fetus at one or more sampling times. the average age of these 17 bulls was about two years greater than the average age of all the bull ...19836357410
[an infection caused by campylobacter fetus]. 19836357724
correlation of an expanded direct fluorescent-antibody system with an established passive hemagglutination system for serogrouping strains of campylobacter jejuni and campylobacter coli.rabbits were inoculated with whole, formalinized cells from eight passive hemagglutination reference strains of campylobacter. fluorescein isothiocyanate-labeled immunoglobulin g from these antisera defined seven new direct fluorescent-antibody serogroups of c. jejuni and one new serogroup of c. coli. this expanded the campylobacter direct fluorescent antibody system to include 17 serogroups of c. jejuni, 3 serogroups of c. coli, and 2 serogroups of c. fetus. we then compared the passive hemaggl ...19836358249
evaluation of filters for recovery of campylobacter jejuni from water.campylobacter jejuni has been incriminated in several large waterborne outbreaks, but it has rarely been isolated from water itself. better methodology is needed for the isolation of c. jejuni from water. we evaluated three types of 0.45-micron microporous filters and three different pore sizes of positively charged depth filters for their ability to recover c. jejuni from seeded, sterile tap and surface water. the microporous filters tested were millipore ha, gelman gn6, and zetapor. three pore ...19836360048
is enrichment culture necessary for the isolation of campylobacter jejuni from faeces?the role of enrichment culture for the isolation of campylobacter jejuni from faeces is discussed. it is concluded that enrichment culture is only necessary for those specimens where it is anticipated that the number of organisms is likely to be low. in a trial of a blood free enrichment broth (ccd broth) and the modified preston enrichment broth the latter gave significantly superior results.19836361073
enteropathogen carriage by healthy individuals living in an area with poor sanitation.faecal carriage of bacterial enteropathogens (enteropathogenic escherichia coli (epec), shigellae and salmonellae) was studied in 265 individuals: 65 infants 3-6 months of age (50 bottle-fed and 15 breast-fed), 100 school-age children 8-10 years of age and 100 adults 21-50 years of age. all were apparently healthy, did not have gastrointestinal symptoms, had not received antibiotics in the preceding fortnight and were not malnourished. enteropathogens were isolated from the faeces of 24 individu ...19836363528
campylobacter jejuni outer membrane proteins are antigenic for humans.all campylobacter jejuni strains have a major outer membrane protein (omp) that migrates between a molecular weight of 41,000 (41k) and 45k and represents more than 50% of protein present, plus several more minor bands. using 125i-radiolabeled c. jejuni cells in a radioimmunoprecipitation procedure to assess whether the omps were antigenic, we studied serum from rabbits immunized with c. jejuni cells, from humans convalescent after c. jejuni infection, and from appropriate controls. in this assa ...19846365789
analysis of campylobacter jejuni antigens with monoclonal antibodies.to develop monoclonal reagents for antigenic analysis and serotyping of campylobacter spp., hybridoma cell lines were produced by fusion of mouse myeloma cells and spleen cells from mice immunized with formalin-treated campylobacter jejuni organisms. an enzyme immunoassay was used for preliminary screening of the cell culture supernatants and ascites. twenty-nine clones which reacted with the immunogen were obtained. seven of these clones were positive in passive hemagglutination tests with shee ...19846365954
is enrichment culture necessary for the isolation of campylobacter jejuni from faeces? 19846368607
procedure for increased recovery of campylobacter jejuni from inoculated unpasteurized milk.different treatments were applied to campylobacter jejuni-inoculated unpasteurized milk to identify means of enhancing the survival of the organism in refrigerated (4 degrees c) samples. the greatest survival occurred in milk supplemented with 0.01% sodium bisulfite and held under an atmosphere of 100% nitrogen (bisulfite-nitrogen), in most instances allowing isolation of c. jejuni from highly contaminated milk 15 or more days longer than from unsupplemented milk held in air (21% oxygen). althou ...19846370137
[an optimal blood culture technic].for blood cultures in septicemic patients it is advisable to use two broth culture bottles. one bottle should be incubated with air, the other without (for anaerobes). supplemented peptone broth ii (becton-dickinson) and bhi roche were superior for culturing aerobic and anaerob bacteria in comparison to other blood culture media. they are commercially available, and with these media it is not necessary to inoculate two bottles with blood from one patient. supplemented peptone broth ii is also su ...19846374431
campylobacter fetus ssp fetus cholecystitis and relapsing bacteremia in a patient with acquired immunodeficiency syndrome.acute gangrenous cholecystitis and relapsing bacteremia caused by campylobacter fetus ssp fetus occurred in a patient with acquired immunodeficiency syndrome. biliary tract colonization probably accounted in part for this unusual phenomenon. whether the patient's deficiency of cell-mediated immunity contributed to his disease is not known.19846377511
detection of campylobacter by immunofluorescence in stools and rectal biopsies of patients with diarrhoea.rabbit antiserum, elicited by the intravenous injection of a strain of campylobacter jejuni heated to 100 degrees c, cross reacted strongly with all other thermophilic campylobacters tested as well as with "c pyloridis" and could be detected by indirect fluorescence with labelled anti-rabbit serum. antisera to formalin killed cells did not do so. the correlation of positive stool culture with positive immunofluorescence of stools and rectal biopsies from patients with diarrhoea was 70-80%. some ...19846381550
intestinal carriage of campylobacter jejuni and salmonella by chicken flocks at slaughter.campylobacter jejuni were isolated in large numbers from the majority of birds sampled in colonic swabs from 28 of 60 flocks at slaughter. by contrast only small numbers of birds from 11 of the same 60 flocks yielded salmonella enteritidis serotypes. three c. jejuni isolates from each flock were serotyped on the basis of their heat-stable antigens, using antisera prepared against 16 serotypes common in campylobacter diarrhea in man. the majority (72 of 83) of the chicken isolates could be seroty ...19846383579
comparison of enrichment methods and atmosphere modification procedures for isolating campylobacter jejuni from foods.a comparison was made of enrichment broths for recovery of campylobacter jejuni from food by the methods of doyle and roman (appl. environ. microbiol. 43:1343-1353) and of park et al. (can. j. microbiol. 27:841-842). no significant differences were found between the results obtained with the two broths. recovery was greater, however, with a constant gas flow into the broths than with an evacuation-replacement method.19846393876
isolation of campylobacter fetus ss jejuni in adult patients with diarrheal syndrome. 19846399196
biphasic culture system for rapid campylobacter cultivation.we developed a biphasic culture system consisting of 4 ml of brucella agar (ba) and 6 ml of brucella broth (bb) in 25-cm2 tissue culture flasks, which were incubated in air (bb/baa) or in a gas mixture of 5% o2, 10% co2, and 85% n2 (bb/bag). these media were also used with a supplement consisting of ferrous sulfate, sodium metabisulfite, and sodium pyruvate and incubated as above (fb/faa and fb/fag, respectively). highly satisfactory growth of campylobacter jejuni 301 was obtained with all mediu ...19836401980
a study of the oxygen and carbon dioxide requirements of thermophilic campylobacters.the oxygen and carbon dioxide requirements of different biotypes of thermophilic campylobacters were investigated by means of (a) quantitative studies, and (b) total growth studies. oxygen tolerance of the five test organisms differed markedly and varied with the carbon dioxide concentration. at most carbon dioxide concentrations tested, campylobacter jejuni strains nctc 11168 and nctc 11392 tolerated 21% oxygen (growth reduced), c coli nctc 11353 tolerated 15% oxygen (growth reduced), and c jej ...19836408142
structure and cell envelope associations of flagellar basal complexes of vibrio cholerae and campylobacter fetus.to isolate intact flagella with basal complexes from vibrio cholerae, a rhamnolipid hemolysin from pseudomonas aeruginosa was used to disrupt the cell envelope and flagellar sheath. the nonionic detergent, triton x-100, provided similar results for campylobacter fetus. each of these basal complexes possessed, in addition to the four classical rings, concentric membrane rings (cmr's) similar to those found in aquaspirillum serpens. through the use of stereo imaging (which allows structures to be ...19846426766
guillain-barré syndrome and campylobacter jejuni: a serological study.the association between campylobacter jejuni infection and guillain-barré syndrome was investigated serologically in a retrospective study of 56 patients admitted to this hospital over four years. evidence of preceding c jejuni infection was found in 21 (38%) of these patients, indicating that c jejuni was the most common single identifiable pathogen precipitating the disease. among those patients who had presented with preceding diarrhoea the serum antibody response was similar to that in uncom ...19846428580
premature labor and neonatal sepsis caused by campylobacter fetus, subsp. fetus--ontario. 19846431256
[campylobacter fetus meningitis in an adult male]. 19846434669
economical, simple method for production of the gaseous environment required for cultivation of campylobacter jejuni.campylobacter jejuni is an enteric pathogen recognized worldwide as a cause of diarrhea. its isolation from stool samples requires a microaerophilic environment that heretofore has been expensive and cumbersome to create. an economical, portable, and simple method is described which involves the production of appropriate concentrations of oxygen and carbon dioxide. inside a plastic bag are placed two cups, one containing fine steel wool (grade 0) previously soaked in a 2.5% aqueous solution of c ...19846436301
[study of bacterial motility and rate of movement using a closed circuit television].speed and motion patterns of campylobacter fetus ssp. jejuni, escherichia coli and pseudomonas aeruginosa were recorded using a closed circuit television camera attached to a phase contrast microscope. a sony video analysis system was used to stop frame videotape at 1/7th and 1/15th. bacterial speeds were: campylobacter 29.2 micron/s, e. coli 8.9 micron/s and p. aeruginosa 16.8 micron/s.19846443639
in vitro susceptibility of campylobacter fetus subsp. jejuni to n-formimidoyl thienamycin, rosaramicin, cefoperazone, and other antimicrobial agents.the activities of 11 antimicrobial agents against 36 strains of campylobacter fetus subsp. jejuni were studied by a broth microdilution method. all strains were susceptible to 7 of the 11 antimicrobial agents. of the newer agents tested n-formimidoyl thienamycin (mk0787) and rosaramicin had very good activity, whereas cefotaxime, moxalactam, and cefoperazone had poorer activity.19816459767
spontaneous bacterial peritonitis due to campylobacter jejuni.a patient with alcoholic cirrhosis complicated by ascites developed spontaneous bacterial peritonitis. campylobacter jejuni--(a microaerophilic bacillus)--was isolated from ascitic fluid and blood.19846463002
a study of campylobacter fetus subsp. jejuni strains isolated from the stools of diarrhoeic children in sri lanka.nine strains of campylobacter fetus subsp. jejuni were isolated from the stools of diarrhoeic children in colombo (sri lanka). the morphology of most of the strains was typical but three strains produced a spreading film of growth. no discrete domed colonies were seen. all were able to grow in a candle jar, and in a medium containing 1% glycine. all produced hydrogen sulphide. all were susceptible to ampicillin, carbenicillin, tetracycline, chloramphenicol, neomycin, erythromycin, gentamicin and ...19846464104
campylobacter fetus subspecies jejuni in senegal. 19846468362
isoprenoid quinone content and cellular fatty acid composition of campylobacter species.a total of 36 strains of campylobacter species were examined for isoprenoid quinones and cellular fatty acids. the isoprenoid quinone content was determined by reverse-phase high-pressure liquid chromatography, and the fatty acids were determined by capillary gas-liquid chromatography. all campylobacter species contained menaquinone-6 (2-methyl-3-farnesyl-farnesyl-1,4-naphthoquinone) and a methyl-substituted menaquinone-6 (2,[5 or 8]-dimethyl-3-farnesyl-farnesyl-1,4-napthoquinone) as the major i ...19846470096
a new bacterial flagellar structure found in campylobacters.a previously unrecognized bacterial flagellar structure present in campylobacter jejuni and campylobacter fetus is described. the structure is located just below the insertion of the flagellar apparatus through the bacterial wall. it is disc shaped, approximately 100 nm in diameter and has eleven spokes radiating from a central hole. the function of this new structure and its possible importance in the taxonomy of campylobacters and other spiral micro-organisms are discussed.19846470668
campylobacter enteritis on hopi and navajo indian reservations. clinical and epidemiologic features.from june 22 through september 30, 1981, stool specimens from 522 hopi and navajo outpatients were cultured because of diarrheal illnesses at the keams canyon indian health service hospital, arizona. campylobacter jejuni was isolated from the specimens of 26 (5%) of the patients. this pathogen was found as frequently as shigella in patients younger than 2 years or older than 20 years, but was significantly less common in the 2 to 20-year age group (p<.000001). campylobacter enteritis was indisti ...19846475040
selected enrichment broths for recovery of campylobacter jejuni from foods.we attempted to shorten the required time for enrichment broth culture for the isolation of campylobacter jejuni. enrichment broths described by doyle and roman and park and stankiewicz and one developed during this study were compared for ability to isolate c. jejuni from raw chicken carcasses. our medium was a modification of that of doyle and roman with the addition of filter-sterilized fbp (0.2% ferrous sulfate, 0.025% sodium metabisulfite, 0.05% sodium pyruvate), 0.1% sodium lauryl sulfate, ...19846476833
differential characteristics of catalase-positive campylobacters correlated with dna homology groups.eighty-four strains of catalase-positive campylobacters could be placed into seven distinct dna homology groups (species), corresponding to campylobacter fetus, "c. hyointestinalis," c. jejuni, c. coli, "c. laridis," "c. fecalis," and aerotolerant campylobacters. the biochemical and physiological characteristics of the strains were examined for their correlation with the homology groups. the characterization tests that provided the most reliable differentiation at the species and subspecies leve ...19846478314
susceptibility of campylobacter fetus subsp. jejuni to paromomycin. 19846489324
campylobacter fetus ssp jejuni as seen in bangalore. 19846490113
new, extended biotyping scheme for campylobacter jejuni, campylobacter coli, and "campylobacter laridis".a biotyping scheme using improved media and methods for the detection of hippurate hydrolysis, rapid h2s production, and dna hydrolysis was applied to 1,826 cultures of campylobacter jejuni, campylobacter coli and "campylobacter laridis" isolates from human and nonhuman sources. four biotypes were identified among c. jejuni: 57.3% of the isolates belonged to biotype i; 36.0%, to biotype ii; 4.0%, to biotype iii; and 2.7%, to biotype iv. c. coli organisms were differentiated into biotype i (67.0% ...19846490850
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