Publications
Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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genome dynamics of campylobacter jejuni in response to bacteriophage predation. | campylobacter jejuni is a leading cause of food-borne illness. although a natural reservoir of the pathogen is domestic poultry, the degree of genomic diversity exhibited by the species limits the application of epidemiological methods to trace specific infection sources. bacteriophage predation is a common burden placed upon c. jejuni populations in the avian gut, and we show that amongst c. jejuni that survive bacteriophage predation in broiler chickens are bacteriophage-resistant types that d ... | 2007 | 17722979 |
discovery of insertion element iscfe1: a new tool for campylobacter fetus subspecies differentiation. | the species campylobacter fetus is divided into the subspecies c. fetus subsp. venerealis (cfv) and c. fetus subsp. fetus (cff). cfv is the causative agent of bovine genital campylobacteriosis, a highly contagious venereal disease that may lead to serious reproductive problems, including sterility and abortion. in contrast, cff can be isolated from the gastrointestinal tract of a wide range of host species, is associated with abortion in sheep and cattle, and can also be isolated from local and ... | 2007 | 17697006 |
development of a real-time fluorescence resonance energy transfer pcr to detect arcobacter species. | a real-time pcr targeting the gyrase a subunit gene outside the quinolone resistance-determining region has been developed to detect arcobacter species. the species identification was done by probe hybridization and melting curve analysis, using fluorescence resonance energy transfer technology. discrimination between arcobacter species was straightforward, as the corresponding melting points showed significant differences with the characteristic melting temperatures of 63.5 degrees c, 58.4 degr ... | 2007 | 17652482 |
a case of mitral endocarditis due to campylobacter fetus subsp. fetus. | this report describes a patient presenting mitral native endocarditis due to campylobacter fetus subsp. fetus, which was revealed by syncope and identified using 16s ribosomal rna gene sequencing. this gene sequencing method has become the preferred approach to identifying the new emerging pathogens when discrepancies exist between phenotypical tests. | 2007 | 17642532 |
high content of proteins containing 21st and 22nd amino acids, selenocysteine and pyrrolysine, in a symbiotic deltaproteobacterium of gutless worm olavius algarvensis. | selenocysteine (sec) and pyrrolysine (pyl) are rare amino acids that are cotranslationally inserted into proteins and known as the 21st and 22nd amino acids in the genetic code. sec and pyl are encoded by uga and uag codons, respectively, which normally serve as stop signals. herein, we report on unusually large selenoproteomes and pyrroproteomes in a symbiont metagenomic dataset of a marine gutless worm, olavius algarvensis. we identified 99 selenoprotein genes that clustered into 30 families, ... | 2007 | 17626042 |
multicenter study to evaluate bloodstream infection by helicobacter cinaedi in japan. | helicobacter cinaedi has being recognized as an important human pathogen which causes bloodstream infections. although the first case of bacteremia with this pathogen in japan was reported in 2003, the true prevalence of h. cinaedi as a pathogen of bloodstream infections in this country is not yet known. therefore, the aim of our study was to assess the incidence of bacteremia with h. cinaedi in japan. we conducted a prospective, multicenter analysis in 13 hospitals during 6 months in tokyo, jap ... | 2007 | 17596362 |
[bacteremia due to campylobacter fetus isolated by conventional methods from an immunocompromised patient]. | the genus campylobacter includes gram-negative, motile, curved rods that can evidence characteristic morphologies. these microorganisms require low oxygen tension and an increased level of co2 for growing. a case of bacteremia due to campylobacter fetus in a patient with a previous diagnosis of breast cancer with metastases in dorso-lumbar column and acute promyelocytic leukemia (fab-m3 variant) is presented. the patient was admitted to our institution due to loss of consciousness and a 2 day--h ... | 2007 | 17585657 |
c-reactive protein collaborates with plasma lectins to boost immune response against bacteria. | although human c-reactive protein (crp) becomes upregulated during septicemia, its role remains unclear, since purified crp showed no binding to many common pathogens. contrary to previous findings, we show that purified human crp (hcrp) binds to salmonella enterica, and that binding is enhanced in the presence of plasma factors. in the horseshoe crab, carcinoscorpius rotundicauda, crp is a major hemolymph protein. incubation of hemolymph with a range of bacteria resulted in crp binding to all t ... | 2007 | 17581635 |
a cyclic amp receptor protein-regulated cell-cell communication system mediates expression of a feca homologue in stenotrophomonas maltophilia. | stenotrophomonas maltophilia wr-c possesses an rpf/diffusible signal factor (dsf) cell-cell communication system. it produces cis-delta2-11-methyl-dodecenoic acid, a dsf, and seven structural derivatives, which require rpff and rpfb for synthesis. acquisition of iron from the environment is important for bacterial growth as well as the expression of virulence genes. we identified a gene homologous to feca, which encodes a ferric citrate receptor that transports exogenous siderophore ferric citra ... | 2007 | 17574998 |
infection of the thyroid gland caused by campylobacter fetus subsp. fetus. | we report what we consider to be the first case of an abscess of the thyroid gland due to campylobacter fetus subsp. fetus (c. fetus) in a patient suffering from hyperthyroidism. c. fetus is known as a rare and opportunistic pathogen in humans, causing a broad variety of systemic infections. acquisition by humans is thought to occur through contact with animals or animal products and to start as a gastro-intestinal colonization. the detection of c. fetus in stool is challenging, since culture ef ... | 2007 | 17547296 |
development of experimental genetic tools for campylobacter fetus. | molecular analysis of the virulence mechanisms of the emerging pathogen campylobacter fetus has been hampered by the lack of genetic tools. we report the development and functional analysis of escherichia coli-campylobacter shuttle vectors that are appropriate for c. fetus. some vectors were constructed based on the known campylobacter coli plasmid pip1455 replicon, which confers a wide host range in campylobacter spp. versatility in directing gene expression was achieved by introducing a strong ... | 2007 | 17513579 |
fatal relapse of a purulent pleurisy caused by campylobacter fetus subsp. fetus. | campylobacter fetus is associated with invasive disease, while other campylobacter species, such as c. coli and c. jejuni, are a common cause of bacterial diarrhea. bacteremia has been well described, but pleurisy remains very uncommon. we report the recurrent isolation of a c. fetus subsp. fetus strain during two episodes of pleural effusion with a fatal outcome. | 2007 | 17507518 |
[campylobacter fetus bacteremia and cellulitis complicating a venous access port infection in an hiv infected patient]. | campylobacter fetus subspecies fetus is an opportunist gram-negative bacillus, which is known to be a cause of systemic infections, mainly in immunocompromised patients. we report a c. fetus bacteremia and cellulitis complicating a venous access port infection in a patient with acquired immunodeficiency syndrome (aids). this bacillus seems to have a predilection for the vascular endothelium and its isolation is difficult. physicians should be aware of c. fetus infection in patients with vascular ... | 2007 | 17446026 |
in vitro activities of cg400549, a novel fabi inhibitor, against recently isolated clinical staphylococcal strains in korea. | the in vitro activities of cg400549, a novel fabi inhibitor, were compared to those of linezolid and commonly used antimicrobials against recent bacterial isolates. cg400549 had an mic(90) of 0.5 microg/ml for staphylococcus aureus strains and was more potent than either linezolid or vancomycin. | 2007 | 17420210 |
thermophilic campylobacter spp. in salad vegetables in malaysia. | the main aim of this study was to combine the techniques of most probable number (mpn) and polymerase chain reaction (pcr) for quantifying the prevalence and numbers of campylobacter spp. in ulam, a popular malaysian salad dish, from a traditional wet market and two modern supermarkets in selangor, malaysia. a total of 309 samples of raw vegetables which are used in ulam were examined in the study. the prevalences of campylobacters in raw vegetables were, for supermarket i, campylobacter spp., 5 ... | 2007 | 17399832 |
campylobacter reactive arthritis: a systematic review. | to review the literature on the epidemiology of campylobacter-associated reactive arthritis (rea). | 2007 | 17360026 |
management of an abdominal aortic aneurysm infected with campylobacter fetus: a case report. | we present a rare case of an abdominal aortic aneurysm (aaa) infected with campylobacter fetus. the patient presented with abdominal pain and leukocytosis, without a palpable aaa. computed tomography (ct) of the abdomen showed a 3.1 x 3.0 cm infrarenal abdominal aneurysm with an extra-aortic fluid collection. at surgery, an in situ graft was placed. intraoperative aortic wall cultures grew pansensitive c. fetus, and blood cultures remained negative. at 9-month follow-up, the patient was doing we ... | 2007 | 17349352 |
[bacterial meningitis with campylobacter fetus manifesting chronic clinical course]. | a 43-year-old man with no past history of underlying disease was admitted to an affiliated hospital, complaining of high fever and severe headache. polynuclear dominant pleocytosis in the cerebrospinal fluid (csf) suggested bacterial meningitis. he was immediately treated with several antibiotics, then his clinical symptoms improved, although intractable headache relapsed after withdrawal of the initial therapy. thereafter he consulted our hospital for a second opinion. csf examination in our ho ... | 2006 | 17323778 |
comparison of disk diffusion and agar dilution methods for erythromycin and ciprofloxacin susceptibility testing of campylobacter jejuni subsp. jejuni. | disk diffusion was a reliable, easy, and inexpensive method for testing the susceptibility of campylobacter jejuni to erythromycin (215 susceptible and 45 resistant isolates) and to ciprofloxacin (154 susceptible, two intermediate, and 124 resistant isolates) using, respectively, an erythromycin disk and ciprofloxacin and nalidixic acid disks. | 2007 | 17261628 |
s-layer anchoring and localization of an s-layer-associated protease in caulobacter crescentus. | the s-layer of the gram-negative bacterium caulobacter crescentus is composed of a single protein, rsaa, that is secreted and assembled into a hexagonal crystalline array that covers the organism. despite the widespread occurrence of comparable bacterial s-layers, little is known about s-layer attachment to cell surfaces, especially for gram-negative organisms. having preliminary indications that the n terminus of rsaa anchors the monomer to the cell surface, we developed an assay to distinguish ... | 2007 | 17209028 |
[bacteraemia due to campylobacter fetus in an immune suppressed patient]. | we report a case of bacteraemia by campylobacter fetus subsp. fetus in a 77 year-old woman with immunosuppression secondary to steroid use. diagnosis was suspected by finding gram negative curved rods in blood cultures taken after 4 days of a febrile illness without local findings. diarrhea was not present. there was no consumption of undercooked meat or non-pasteurized milk and no contact with pets. the patient was treated with sulbactam-cefoperazone due to the coexistence of urinary tract infe ... | 2006 | 17186081 |
oxygen reactivity of both respiratory oxidases in campylobacter jejuni: the cydab genes encode a cyanide-resistant, low-affinity oxidase that is not of the cytochrome bd type. | the microaerophilic bacterium campylobacter jejuni is a significant food-borne pathogen and is predicted to possess two terminal respiratory oxidases with unknown properties. inspection of the genome reveals an operon (cydab) apparently encoding a cytochrome bd-like oxidase homologous to oxidases in escherichia coli and azotobacter vinelandii. however, c. jejuni cells lacked all spectral signals characteristic of the high-spin hemes b and d of these oxidases. mutation of the cydab operon of c. j ... | 2007 | 17172349 |
oxygen reactivity of both respiratory oxidases in campylobacter jejuni: the cydab genes encode a cyanide-resistant, low-affinity oxidase that is not of the cytochrome bd type. | the microaerophilic bacterium campylobacter jejuni is a significant food-borne pathogen and is predicted to possess two terminal respiratory oxidases with unknown properties. inspection of the genome reveals an operon (cydab) apparently encoding a cytochrome bd-like oxidase homologous to oxidases in escherichia coli and azotobacter vinelandii. however, c. jejuni cells lacked all spectral signals characteristic of the high-spin hemes b and d of these oxidases. mutation of the cydab operon of c. j ... | 2007 | 17172349 |
capa, an autotransporter protein of campylobacter jejuni, mediates association with human epithelial cells and colonization of the chicken gut. | two putative autotransporter proteins, capa and capb, were identified in silico from the genome sequence of campylobacter jejuni nctc11168. the genes encoding each protein contain homopolymeric tracts, suggestive of phase variation mediated by a slipped-strand mispairing mechanism; in each case the gene sequence contained frameshifts at these positions. the c-terminal two-thirds of the two genes, as well as a portion of the predicted signal peptides, were identical; the remaining n-terminal port ... | 2007 | 17172331 |
capa, an autotransporter protein of campylobacter jejuni, mediates association with human epithelial cells and colonization of the chicken gut. | two putative autotransporter proteins, capa and capb, were identified in silico from the genome sequence of campylobacter jejuni nctc11168. the genes encoding each protein contain homopolymeric tracts, suggestive of phase variation mediated by a slipped-strand mispairing mechanism; in each case the gene sequence contained frameshifts at these positions. the c-terminal two-thirds of the two genes, as well as a portion of the predicted signal peptides, were identical; the remaining n-terminal port ... | 2007 | 17172331 |
temperate bacteriophages affect pulsed-field gel electrophoresis patterns of campylobacter jejuni. | the recently sequenced genome of campylobacter jejuni rm1221 revealed the presence of three integrated bacteriophage-like elements. in this study, genes from the first element, a mu-like bacteriophage, were amplified by pcr and used to probe pulsed-field gels of clinical c. jejuni strains obtained from a waterborne outbreak (ontario, canada, 2000). these highly similar strains differed only by their pulsed-field gel electrophoresis (pfge) patterns due to an apparent insertion or deletion of a 40 ... | 2007 | 17135440 |
temperate bacteriophages affect pulsed-field gel electrophoresis patterns of campylobacter jejuni. | the recently sequenced genome of campylobacter jejuni rm1221 revealed the presence of three integrated bacteriophage-like elements. in this study, genes from the first element, a mu-like bacteriophage, were amplified by pcr and used to probe pulsed-field gels of clinical c. jejuni strains obtained from a waterborne outbreak (ontario, canada, 2000). these highly similar strains differed only by their pulsed-field gel electrophoresis (pfge) patterns due to an apparent insertion or deletion of a 40 ... | 2007 | 17135440 |
specific detection of campylobacter jejuni from faeces using single nucleotide polymorphisms. | specimens of human faeces were tested by a rapid strategy for detection of campylobacter jejuni lineages by the presence of specific single nucleotide polymorphisms (snps) based on the c. jejuni multi locus sequence typing (mlst) scheme. this strategy was derived from analysis of the mlst databases to identify clonal complex specific snps followed by the design of real-time pcr assays to enable identification of six major c. jejuni clonal complexes associated with cases of human infection. the o ... | 2007 | 17109769 |
specific detection of campylobacter jejuni from faeces using single nucleotide polymorphisms. | specimens of human faeces were tested by a rapid strategy for detection of campylobacter jejuni lineages by the presence of specific single nucleotide polymorphisms (snps) based on the c. jejuni multi locus sequence typing (mlst) scheme. this strategy was derived from analysis of the mlst databases to identify clonal complex specific snps followed by the design of real-time pcr assays to enable identification of six major c. jejuni clonal complexes associated with cases of human infection. the o ... | 2007 | 17109769 |
cellulitis and septic arthritis caused by campylobacter fetus and campylobacter jejuni: report of 2 cases and review of the literature. | bacteremia caused by gram-negative bacteria occasionally causes soft tissue infections, including cellulitis and septic arthritis. we describe 1 patient each with campylobacter fetus cellulitis and septic arthritis and review the world literature with regard to c. fetus and c. jejuni infections at these sites. altogether, 14 patients with cellulitis (12 due to c. fetus and 2 due to c. jejuni) and 20 patients with septic arthritis (15 due to c. fetus and 4 caused by c. jejuni) have been described ... | 2003 | 17043445 |
first case of bacteremia and multifocal cellulitis due to helicobacter canis in an immunocompetent patient. | bacteremia due to helicobacter canis has been reported in a patient with x-linked hypogammaglobulinemia. here we report on the first human case of h. canis bacteremia in an immunocompetent host. identification of the organism was made by genetic and phylogenetic analyses of the complete 16s rrna sequence. | 2006 | 17005753 |
broilers do not play a dominant role in the campylobacter fetus contamination of humans. | campylobacter fetus causes severe infections in humans and can be isolated from various mammals and reptiles. however, although poultry are considered to be the main reservoir of campylobacter jejuni, little is known about the presence of c. fetus in poultry. thus, specific pathogen-free chickens were experimentally inoculated with a mixture of either three non-thermotolerant or four thermotolerant human strains of c. fetus. faecal samples were regularly sampled after inoculation and caeca and i ... | 2006 | 16914660 |
an outbreak of salmonella abortusovis abortions in sheep in south croatia. | the aim of the study was to give an account of the epidemic of abortions in sheep caused by salmonella enterica ssp. enterica serovar abortusovis, which occurred in dalmatia, south croatia, in winter 2003-2004. five sheep flocks with rate of abortion ranging from 22% to 38% during the last-third of gestation were examined. salmonella abortusovis was isolated from 13 vaginal smears and two fetuses. direct inoculation was found to be superior to pre-enrichment and enrichment in selective broth for ... | 2006 | 16907961 |
detection sensitivity and quantitation of plasmodium falciparum var gene transcripts by real-time rt-pcr in comparison with conventional rt-pcr. | antigenic variation in plasmodium falciparum erythrocyte membrane protein 1, caused by a switch in transcription of the encoding var gene, is an important feature of malaria. in this study, we quantified the relative abundance of var gene transcripts present in p. falciparum parasite clones using real-time reverse transcription-polymerase chain reaction (rt-pcr) and conventional rt-pcr combined with cloning and sequencing, with the aim of directly comparing the results obtained. when there was s ... | 2006 | 16896121 |
phase variation and genomic architecture changes in azospirillum. | the plant growth-promoting rhizobacterium azospirillum lipoferum 4b generates in vitro at high frequency a stable nonswimming phase variant designated 4v(i), which is distinguishable from the wild type by the differential absorption of dyes. the frequency of variants generated by a reca mutant of a. lipoferum 4b was increased up to 10-fold. the pleiotropic modifications characteristic of the phase variant are well documented, but the molecular processes involved are unknown. here, the objective ... | 2006 | 16855225 |
identification of campylobacter fetus subspecies by phenotypic differentiation and pcr. | the species campylobacter fetus is divided into the subspecies c. fetus subsp. venerealis and c. fetus subsp. fetus, which differ in their epidemiologies and clinical importance. the differences between these subspecies make accurate distinction between the two essential. first, the value of seven key tests for the traditional differentiation of c. fetus was investigated. afterwards, the results of the phenotypic differentiation and pcr were compared to address the question of the reliability of ... | 2006 | 16757592 |
temporal prevalence of antimicrobial resistance in campylobacter spp. from beef cattle in alberta feedlots. | antimicrobial resistance (amr) was temporally assessed in campylobacters isolated from beef cattle (7,738 fecal samples from 2,622 animals) in four commercial feedlots in alberta. all calves were administered chlortetracycline and oxytetracycline in feed, and a majority of the animals (93%) were injected with long-acting oxytetracycline upon arrival at the feedlot. fecal samples from individual animals were collected upon arrival (i.e., entry sample), 69 days (standard deviation [sd] = 3 days) a ... | 2006 | 16751519 |
reproductive performance of beef heifers: effects of vulvo-vaginitis, ureaplasma diversum and prebreeding antibiotic administration. | a group of 450 heifers, 13 to 15 mo of age, were individually identified, vaccinated (ibr, pi(3), leptospira, campylobacter fetus), examined (body condition score, reproductive tract evaluation, assessment of vaginal lesions), and cultured for u. diversum . heifers were randomly allocated to either a treated group given chlortetracycline (approximately 350 mg/hd/d for 30 d in a grain crumble) or a nontreated control group. prebreeding, most heifers showed signs of vulvovaginitis, 44% cultured po ... | 1993 | 16727333 |
the use of loglinear model to evaluate factors associated with sero-prevalence of campylobacter fetus in dairy cattle. | campylobacter fetus is one of the leading infections causing bovine infertility and abortion. in a cross-sectional study, the associations of parity, herd, serological status against leptospira hardjo and haemophilus somnus with the sero-prevalence of campylobacter fetus in dairy cattle were evaluated. four hundred dairy cows from three herds were included in the study. blood samples from the cows were collected in december 1986. an enzyme-linked immunosorbant assay (elisa) was used to determine ... | 1990 | 16726898 |
a new antibiotic combination for frozen bovine semen 1. control of mycoplasmas, ureaplasmas, campylobacter fetus subsp. venerealis and haemophilus somnus. | systematic evaluations of new combinations of antibiotics for the control of bovine mycoplasmas, ureaplasmas, campylobacter fetus subsp. venerealis and haemophilus somnus in a bovine frozen semen process were made. these organisms were standardized to 10(5) to 10(6) colony forming unit (cfu) and inoculated into each ml of raw semen. antibiotics in a final volume of 0.02 ml were added to each ml of the raw semen and were contained at the same concentration in the nonglycerol portion of the extend ... | 1988 | 16726381 |
outbreaks of waterborne infectious intestinal disease in england and wales, 1992-2003. | we reviewed the epidemiological and microbiological characteristics of 89 reported outbreaks of waterborne infectious intestinal disease affecting 4321 people in england and wales over the period 1992-2003. public water supplies were implicated in 24 outbreaks (27%), private water supplies in 25 (28%), swimming pools in 35 (39%) and other sources in five outbreaks (6%). cryptosporidium was implicated in 69% of outbreaks, campylobacter sp. in 14%, giardia in 2%, e. coli o157 in 3% and astrovirus ... | 2006 | 16690002 |
campylobacter curvus-associated hepatic abscesses: a case report. | campylobacter curvus was isolated from blood cultures of a patient with liver abscesses. bacterial identification involved gram staining, biochemical analysis, gas-liquid chromatography, and 16s rrna sequencing. the difficulty in isolation, identification, and growth of the species confirms previous work that these organisms may be overlooked by conventional detection methods. | 2006 | 16672438 |
global detection and identification of campylobacter fetus subsp. venerealis. | bovine genital campylobacteriosis caused by campylobacter fetus subsp. venerealis (cfv) is a genital infection that threatens the cattle industry. detection and identification of cfv are key factors in control programmes. trade regulations should be based on scientifically and internationally accepted methods of detection and identification of cfv. such methods are described in the world organisation for animal health (oie) manual of diagnostic tests and vaccines for terrestrial animals. a study ... | 2005 | 16642772 |
prevalence of genital campylobacteriosis and trichomonosis in crossbred breeding bulls kept on zero-grazed smallholder dairy farms in the tanga region of tanzania. | a survey to demonstrate the presence or absence of genital campylobacteriosis and trichomonosis in cross-bred breeding bulls kept under smallholding dairy farms in the tanga region of tanzania was carried out during the period of january-june 1996. sheath washings, swabs and preputial scrapings were collected from 58 randomly selected bulls. campylobacter fetus subsp. venerealis was demonstrated in 3/58 (5.1%) and tritrichomonas foetus in 0/58 (0%) of all bulls tested. bull-level variables of le ... | 2005 | 16642720 |
changes in flagellin glycosylation affect campylobacter autoagglutination and virulence. | analysis of the complete flagellin glycosylation locus of campylobacter jejuni strain 81-176 revealed a less complex genomic organization than the corresponding region in the genome strain, c. jejuni nctc 11168. twenty-four of the 45 genes found between cj1293 and cj1337 in nctc 11168 are missing in 81-176. mutation of six new genes, in addition to three previously reported, resulted in a non-motile phenotype, consistent with a role in synthesis of pseudaminic acid (pseac) or transfer of pseac t ... | 2006 | 16573682 |
a selective medium for the isolation of vibrio fetus and related vibrios. | 1956 | 16561828 | |
the effects of vibrio fetus on the growth of trichomonas foetus (protozoa). | 1948 | 16561439 | |
the apparent involvement of vibrio fetus in an infection of man. | 1948 | 16561425 | |
bovine venereal vibriosis: variations in immunoglobulin class of antibodies in genital secretions and serum. | the immunoglobulin classes of antibodies to campylobacter (vibrio) fetus in cervicovaginal mucus (cvm) were determined by the indirect fluorescent antibody test at sequential periods, since the order of class appearance has not been established for specific secretory immune responses. in the local immune response to c. fetus immunoglobulin m (igm) antibodies appeared first, immunoglobulin a (iga) antibodies next, and immunoglobulin g (igg) last. igm antibodies were quite transient, but igg antib ... | 1974 | 16558094 |
location of vibrio fetus var. venerealis within the endometrium of the cow. | vibrio fetus was located by immunofluorescence within the endometrial epithelium of experimentally infected heifers. bacterium-like structures were also located by electron microscopy within endometrial epithelial cells. | 1971 | 16558068 |
rate of clearance of vibrio fetus var. venerealis in the rabbit. | the rate of clearance of vibrio fetus var. venerealis was measured by using in vivo and in vitro methods. the rate of clearance from the circulating blood increased in immune rabbits and reached a plateau 4 days after immunization. thereafter in vivo clearance did not increase further despite rising agglutinin titers. vibriocidal activity of freshly drawn blood paralleled the rate of in vivo clearance although it was less effective at 4 days. vibriocidal activity of immune serum was not demonstr ... | 1971 | 16558060 |
purification and partial characterization of a vibrio fetus immunogen. | an antigen released into broth culture medium (2 mg/100 ml of culture medium) during 20-hr growth of vibrio fetus was removed from the growth medium by salt precipitation [50 g of (nh(4))(2)so(4)/100 ml of broth] and centrifugation. about 30 mg of the postgrowth broth (pgb) antigen protein was removed from other salt precipitable material during one polyacrylamide gel electrophoretic run. the pgb antigen was further purified by using gel filtration on sephadex g-200, and a molecular weight of 13 ... | 1971 | 16558018 |
bactericidal effect and serological response of blood and secretions in bovine vibriosis. | udder infection of vaccinated heifers with vibrio fetus var. venerealis led to an early and severe reaction consisting of local swelling, hyperthermia, and increased blood leukocyte counts. this reaction was absent or less pronounced in heifers not previously vaccinated. this was interpreted as an immediate hypersensitivity reaction elicited in the vaccinated heifers. specific antibodies were found in udder exudate from vaccinated heifers. immune serum and udder exudate were moderately bacterici ... | 1971 | 16557987 |
a case of perinatal sepsis by campylobacter fetus subsp. fetus infection successfully treated with carbapenem--case report and literature review. | infection due to campylobacter fetus subsp. fetus during pregnancy is uncommon in humans. we report a case of a pregnant woman who experienced premature labor. the infant was diagnosed with neonatal sepsis due to c. fetus subsp. fetus, and was successfully treated with carbapenem. maternal clinical symptoms and severe villitis suggested that the route of fetal infection was hematogenous spread. we also review previous reports in the literature that describe this infection during pregnancy. | 2006 | 16542730 |
antimicrobial susceptibility of clinical isolates of non-jejuni/coli campylobacters and arcobacters from belgium. | to determine the susceptibility of non-jejuni/coli campylobacters and arcobacters isolated from diarrhoeal stool specimens in belgium. | 2006 | 16533825 |
veterinary and medical aspects of abortion in danish sheep. | the danish sheep population totals around 144,000 animals, but little is known of the causes and prevalance of diseases. this study focuses on the causes of abortion in danish sheep. during one breeding season, aborted foetuses and stillbirths with signs of intrauterine death or malformation were submitted for laboratory examination from a population of 3,758 breeding ewes. samples from 24 incidents of abortion and 21 ewes delivering malformed lambs or lambs with ante partum decomposition were s ... | 2006 | 16519752 |
comparison of culture and a novel 5' taq nuclease assay for direct detection of campylobacter fetus subsp. venerealis in clinical specimens from cattle. | a campylobacter fetus subsp. venerealis-specific 5' taq nuclease pcr assay using a 3' minor groove binder-dna probe (taqman mgb) was developed based on a subspecies-specific fragment of unknown identity (s. hum, k. quinn, j. brunner, and s. l. on, aust. vet. j. 75:827-831, 1997). the assay specifically detected four c. fetus subsp. venerealis strains with no observed cross-reaction with c. fetus subsp. fetus-related campylobacter species or other bovine venereal microflora. the 5' taq nuclease a ... | 2006 | 16517880 |
host range and emerging and reemerging pathogens. | an updated literature survey identified 1,407 recognized species of human pathogen, 58% of which are zoonotic. of the total, 177 are regarded as emerging or reemerging. zoonotic pathogens are twice as likely to be in this category as are nonzoonotic pathogens. emerging and reemerging pathogens are not strongly associated with particular types of nonhuman hosts, but they are most likely to have the broadest host ranges. emerging and reemerging zoonoses are associated with a wide range of drivers, ... | 2005 | 16485468 |
pulsed-field gel electrophoresis of campylobacter jejuni sheep abortion isolates. | campylobacter species are a significant cause of sheep abortion in most sheep-raising countries. in new zealand, campylobacter fetus subsp. fetus is the leading cause of diagnosed sheep abortion and the species c. jejuni and c. coli have also been implicated. to date, strain typing information of c. jejuni sheep abortion isolates is limited. the objective of the present study was to genotype c. jejuni isolates cultured from sheep abortions submitted to diagnostic laboratories in new zealand duri ... | 2006 | 16472946 |
bacterial biota in reflux esophagitis and barrett's esophagus. | to identify the bacterial flora in conditions such as barrettos esophagus and reflux esophagitis to determine if they are similar to normal esophageal flora. | 2005 | 16437628 |
screening of feral pigeon (colomba livia), mallard (anas platyrhynchos) and graylag goose (anser anser) populations for campylobacter spp., salmonella spp., avian influenza virus and avian paramyxovirus. | a total of 119 fresh faecal samples were collected from graylag geese migrating northwards in april. also, cloacal swabs were taken from 100 carcasses of graylag geese shot during the hunting season in august. in addition, samples were taken from 200 feral pigeons and five mallards. the cultivation of bacteria detected campylobacter jejuni jejuni in six of the pigeons, and in one of the mallards. salmonella diarizona 14: k: z53 was detected in one graylag goose, while all pigeons and mallards we ... | 2005 | 16398331 |
a bacillus thuringiensis s-layer protein involved in toxicity against epilachna varivestis (coleoptera: coccinellidae). | the use of bacillus thuringiensis as a biopesticide is a viable alternative for insect control since the insecticidal cry proteins produced by these bacteria are highly specific; harmless to humans, vertebrates, and plants; and completely biodegradable. in addition to cry proteins, b. thuringiensis produces a number of extracellular compounds, including s-layer proteins (slp), that contribute to virulence. the s layer is an ordered structure representing a proteinaceous paracrystalline array whi ... | 2006 | 16391064 |
clonal nature of campylobacter fetus as defined by multilocus sequence typing. | campylobacter fetus can be divided into the subspecies c. fetus subsp. fetus and c. fetus subsp. venerealis. c. fetus subsp. fetus causes sporadic infections in humans and abortion in cattle and sheep and has been isolated from a variety of sites in different hosts. c. fetus subsp. venerealis is host restricted, being isolated mainly from the genital tracts of cattle, and is the causative agent of bovine genital campylobacteriosis. despite differences in niche preference, microbiological subspec ... | 2005 | 16333072 |
infective tricuspid valve endocarditis with pulmonary emboli caused by campylobacter fetus after tooth extraction. | we describe a case of infective endocarditis caused by campylobacter fetus accompanied by pulmonary emboli. a 52-year-old man was referred to our hospital due to febrile temperatures with a history of dental treatment followed by eating raw meat. computed tomography revealed multiple infiltrations and a nodule with low attenuation area and feeding vessels. a mobile mass, possible vegetation, attached to the tricuspid valve was detected by transthoracic echocardiography. two blood cultures disclo ... | 2005 | 16293916 |
campylobacter fetus bacteraemia in a healthy individual: clinical and therapeutical implications. | campylobacter species are well-recognized common causes of gastrointestinal infections. while campylobacter jejuni is probably the most common campylobacter isolated in humans, campylobacter fetus is rather infrequent and mostly related with bacteraemia. even on such occasions, it seems that immunocompetent individuals are spared. we report a case of c. fetus bacteraemia in a healthy-except for impaired fasting glucose (ifg) levels-farmer, presenting as an acute febrile syndrome and treated succ ... | 2005 | 16291287 |
molecular epidemiology of campylobacter fetus subsp. fetus on bovine artificial insemination stations using pulsed field gel electrophoresis. | the presence of campylobacter fetus subspecies fetus (cff) on bovine artificial insemination (ai)-stations can have major economical consequences. more knowledge on the epidemiology of c. fetus is needed to control cff infections at ai-stations. we assessed the epidemiology of cff on ai-stations and the molecular relationship between cff strains isolated from outbreaks on ai-stations. thirteen cff strains (two cff strains per outbreak and one sporadic case) isolated from bulls housed on differen ... | 2006 | 16289638 |
amplified fragment length polymorphism based identification of genetic markers and novel pcr assay for differentiation of campylobacter fetus subspecies. | differentiation of campylobacter fetus into c. fetus subsp. fetus (cff) and c. fetus subsp. venerealis (cfv) is important for both clinical and economic reasons. in the past, several molecular typing methods have been used for differentiation, including amplified fragment length polymorphism (aflp). in this study, aflp was employed to identify c. fetus subspecies specific markers that can serve as a basis for design of novel pcr primer sets for cfv. four groups of c. fetus strains with different ... | 2005 | 16278437 |
validation of a monoclonal antibody-based capture enzyme-linked immunosorbent assay for detection of campylobacter fetus. | a monoclonal antibody (mab)-based antigen capture enzyme-linked immunosorbent assay (elisa) was compared with the routine culture methodology for the detection of campylobacter fetus subspecies from bovine and ovine field samples inoculated into clark's transport enrichment medium (tem). the work was a collaboration between two different diagnostic laboratories, one in canada and the other in england. in both labs, tem samples were incubated for 4 days at 35 degrees c and then tested by culture ... | 2005 | 16275938 |
salmonella in black-headed gulls ( larus ridibundus); prevalence, genotypes and influence on salmonella epidemiology. | during a period of 3 years, 1998-2000, 1047 faecal swabs from black-headed gulls were sampled at one location in southern sweden. salmonella spp. was found in 28 individuals (2.7%) and the dominating serotype found was s. typhimurium (83%). twenty-five per cent of the salmonella-infected gulls were later recaptured and re-sampled. we found that salmonella infection in black-headed gulls was of short duration, and that infection in this bird species was predominantly expressed as carriage without ... | 2006 | 16238820 |
salmonella in black-headed gulls ( larus ridibundus); prevalence, genotypes and influence on salmonella epidemiology. | during a period of 3 years, 1998-2000, 1047 faecal swabs from black-headed gulls were sampled at one location in southern sweden. salmonella spp. was found in 28 individuals (2.7%) and the dominating serotype found was s. typhimurium (83%). twenty-five per cent of the salmonella-infected gulls were later recaptured and re-sampled. we found that salmonella infection in black-headed gulls was of short duration, and that infection in this bird species was predominantly expressed as carriage without ... | 2006 | 16238820 |
contribution of systematic serological testing in diagnosis of infective endocarditis. | despite progress with diagnostic criteria, the type and timing of laboratory tests used to diagnose infective endocarditis (ie) have not been standardized. this is especially true with serological testing. patients with suspected ie were evaluated by a standard diagnostic protocol. this protocol mandated an evaluation of the patients according to the modified duke criteria and used a battery of laboratory investigations, including three sets of blood cultures and systematic serological testing f ... | 2005 | 16207989 |
the frequency and rate of pilin antigenic variation in neisseria gonorrhoeae. | the pilin antigenic variation (av) system of neisseria gonorrhoeae (gc) mediates unidirectional dna recombination from silent gene copies into the pilin expression locus. a dna sequencing assay was developed to accurately measure pilin av in a population of gc strain fa1090 arising from a defined pilin progenitor under non-selective culture conditions. this assay employs a piliated parental gc variant with a reca allele whose promoter is replaced by lac-regulatory elements, allowing for controll ... | 2005 | 16194236 |
jejunal hemorrhage syndrome in dairy and beef cattle: 11 cases (2001 to 2003). | the medical records of 11 cattle with jejunal hemorrhage syndrome were reviewed. female and male, lactating and pregnant, dairy and beef cattle were affected. decreased feed intake and milk production, reduced amounts of dark feces, and abdominal discomfort were common historical findings. common clinical findings included depressed demeanor, a "ping" and fluid-splashing sounds over the right abdomen, melena, and distended loops of intestine on rectal palpation. surgery was done on 7 cases, 10 c ... | 2005 | 16187715 |
a multiplex polymerase chain reaction to detect and differentiate campylobacter fetus subspecies fetus and campylobacter fetus -species venerealis: use on uk isolates of c. fetus and other campylobacter spp. | subspeciation of campylobacter fetus subsp. fetus (cff) and campylobacter fetus subsp. venerealis (cfv) is important for international animal import regulations. phenotyping can be unreliable, and genotyping by techniques like pulsed field gel electrophoresis is difficult in routine diagnostic laboratories. a pcr subspeciation technique has been reported [aust vet j (1997) 75, 827]; we aimed to develop this pcr and investigate its use on uk c. fetus isolates. | 2005 | 16162226 |
factors affecting the pressure resistance of some campylobacter species. | to compare pressure resistance between strains of campylobacter jejuni, campylobacter coli, campylobacter lari and campylobacter fetus, and to investigate the effect of suspending medium on pressure resistance of sensitive and more resistant strains. | 2005 | 16162138 |
does age acquired immunity confer selective protection to common serotypes of campylobacter jejuni? | campylobacter infection is a major cause of bacterial gastrointestinal disease. exposure to campylobacter is known to produce an immune response in humans that can prevent future symptomatic infections. further, studies of the general population have shown that seroprevalence to campylobacter increases with age. | 2005 | 16117832 |
campylobacter spp., salmonella spp., verocytotoxic escherichia coli, and antibiotic resistance in indicator organisms in wild cervids. | faecal samples were collected, as part of the national health surveillance program for cervids (hop) in norway, from wild red deer, roe deer, moose and reindeer during ordinary hunting seasons from 2001 to 2003. samples from a total of 618 animals were examined for verocytotoxic e. coli (vtec); 611 animals for salmonella and 324 animals for campylobacter. a total of 50 samples were cultivated from each cervid species in order to isolate the indicator bacterial species e. coli and enterococcus fa ... | 2005 | 16108210 |
real-time pcr screening for 16s rrna mutations associated with resistance to tetracycline in helicobacter pylori. | the effectiveness of recommended first-line therapies for helicobacter pylori infections is decreasing due to the occurrence of resistance to metronidazole and/or clarithromycin. quadruple therapies, which include tetracycline and a bismuth salt, are useful alternative regimens. however, resistance to tetracycline, mainly caused by mutations in the 16s rrna genes (rrna and rrnb) affecting nucleotides 926 to 928, are already emerging and can impair the efficacies of such second-line regimens. her ... | 2005 | 16048919 |
[campylobacter as a venereal disease in cattle]. | since the introduction of ai, venereal diseases caused by tritrichomonas fetus and campylobacter fetus subsp. venerealis haved been eradicated in the netherlands. campylobacter fetus subsp. fetus can cause sporadic abortion and early embryonic death. when natural breeding is practised, venereal diseases must be included in the list of differential diagnoses of fertility problems. a case study of a campylobacter fetus subsp. fetus infection is described. | 2005 | 16047745 |
pulsed-field gel electrophoresis typing of campylobacter fetus subsp. fetus from sheep abortions in the hawke's bay region of new zealand. | to type campylobacter isolates from sheep abortions from the hawke's bay region of new zealand. | 2003 | 16032287 |
campylobacter fetus fetus abortions in vaccinated ewes - vaccine history and ongoing development. | 2000 | 16032146 | |
campylobacter fetus fetus abortions in vaccinated ewes. | to investigate the cause of an outbreak of ovine abortion in 1996 in a flock of 300 two-tooth (rising 2-year-old) ewes vaccinated against campylobacter fetus fetus infection and to subsequently characterise the strain of c. fetus fetus isolated from aborted foetuses. | 2000 | 16032144 |
attempted definition by immunoblotting of the causes of reactivity in suspected false-positive sera in the brucella ovis complement fixation test. | seventy-nine suspected false-positive sera, obtained over 1 year from routine submissions for brucella ovis serological testing, were used in this study. these sera, which exhibited titres in the complement fixation test, but which because of their epidemiological history and their reactions in the enzyme-linked immunosorbent assay and gel diffusion test were suspected to be false positives, were further analysed by immunoblotting. in blots, using b. ovis antigens, rough lipopolysaccharide was i ... | 1996 | 16031926 |
tactical control of ovine campylobacter abortion outbreaks with a bacterin. | abortions due to campylobacter fetus fetus (c. fetus fetus) were diagnosed in three canterbury ewe flocks 6 weeks prior to lambing. in two of the flocks, two inoculations of a c. fetus fetus bacterin, 10 days apart, reduced the incidence of abortions in the treated ewes to about one third and one half respectively of the level in the control ewes in the same flock. the treatment had no effect in a third flock where an outbreak had been in progress for 2 weeks before investigations started. the r ... | 1996 | 16031895 |
typing of campylobacter fetus fetus isolated from sheep abortions in n.z. | 1985 | 16031180 | |
typing of campylobacter fetus fetus isolated from sheep abortions in new zealand. | 1985 | 16031151 | |
serology of campylobacter fetus fetus strains from four outbreaks of ovine abortion. | as a result of a ministry of agriculture & fisheries survey on ovine abortions, 76 isolates of campylobacterfetus fetus were obtained. these isolates were from four farms in the southern hawkes bay, with an abortion incidence of 2.8% to 9.1%. antisera to eight different strains of c. fetus fetus were made in rabbits. strains were then examined using whole cell tube agglutination tests and sensitised staphylococcal protein a slide agglutination tests. heat labile antigens were examined by absorbi ... | 1984 | 16031007 |
transport media for campylobacter fetus venerealis. | 1982 | 16030854 | |
campylobacter surface-layers (s-layers) and immune evasion. | many pathogenic bacteria have evolved mechanisms for evading host immune systems. one evasion mechanism is manifest by the surface layer (s-layer), a paracrystalline protein structure composed of s-layer proteins (slps). the s-layer, possessed by 2 campylobacter species (c. fetus and c. rectus), is external to the bacterial outer membrane and can have multiple functions in immune avoidance. c. fetus is a pathogen of ungulates and immunocompromised humans, in whom it causes disseminated bloodstre ... | 2002 | 16013216 |
genetic divergence of campylobacter fetus strains of mammal and reptile origins. | campylobacter fetus is a gram-negative bacterial pathogen of both humans and animals. two subspecies have been identified, campylobacter fetus subsp. fetus and campylobacter fetus subsp. venerealis, and there are two serotypes, a and b. to further investigate the genetic diversity among c. fetus strains of different origins, subspecies, and serotypes, we performed multiple genetic analyses by utilizing random amplification of polymorphic dna (rapd), pulsed-field gel electrophoresis (pfge), and d ... | 2005 | 16000457 |
mechanisms underlying campylobacter fetus pathogenesis in humans: surface-layer protein variation in relapsing infections. | campylobacter fetus causes gastrointestinal and systemic infections in humans. although relapse is common despite antibiotic treatment, the mechanisms are not well understood. the surface-layer proteins (slps) of c. fetus, which are critical in virulence, undergo high-frequency phenotypic switching due to recombination of sap homologues, resulting in antigenic variation. to investigate the mechanisms involved in relapsing c. fetus infections, we compared slp variation in 4 pairs of c. fetus stra ... | 2005 | 15897994 |
immunohistochemical identification of campylobacter fetus in natural cases of bovine and ovine abortions. | an immunohistochemistry (ihc) procedure for the detection of campylobacter fetus antigens using an avidin-biotin complex technique was performed on formalin fixed bovine and ovine fetal tissues from 26 natural cases of campylobacter spp. abortion (four ovine and 22 bovine). the species of campylobacter isolated included c. fetus ssp. venerealis from 13 bovine fetuses, c. fetus ssp. fetus from two ovine and one bovine fetus, campylobacter jejuni from seven bovine fetuses, campylobacter lari from ... | 2005 | 15876227 |
some effects of experimental vibrio fetus (venerealis) infection on cattle inoculated with a commercial bacterin. | four groups of ten heifers were used to study the effect of a commercial vibrio fetus bacterin on the development of vaginal mucus agglutination (vma) reactions and subsequent reproductive performance in the presence of active v. fetus infection. two groups were vaccinated and one of these was infected one month later. the other two groups were not vaccinated and one of these was infected. it was found that in the absence of infection the bacterin did not produce diagnostically significant vma r ... | 1968 | 15846896 |
bilateral prosthetic knee infection by campylobacter fetus. | we present the first documented case of a bilateral prosthetic knee joint infection with campylobacter fetus. our patient's risk factors included age, the presence of prosthetic joints, and potential exposure through his contact with farm animals. it is important to be aware of the possibility of c fetus joint infections in high-risk patients who present with pain after total joint arthroplasty. | 2005 | 15809962 |
campylobacter fetus prosthetic hip joint infection: successful management with device retention and review. | 2005 | 15780423 | |
pulsed-field gel electrophoresis typing of campylobacter fetus subsp. fetus isolated from sheep abortions in new zealand. | to genotype campylobacter fetus subsp. fetus isolates cultured from sheep abortions submitted to diagnostic laboratories in new zealand during the year 2000 breeding season. to compare the types found nationally with those found in the hawke's bay region in 1999, and strains held in the new zealand reference culture collection, medical section (nzrm) from a study published in 1987. | 2004 | 15768136 |
the need to differentiate campylobacter fetus subspecies isolated from humans. | 2005 | 15760436 | |
development of a real-time fluorescence resonance energy transfer pcr to identify the main pathogenic campylobacter spp. | a simple real-time fluorescence resonance energy transfer (fret) pcr, targeting the gyra gene outside the quinolone resistance-determining region, was developed to identify campylobacter jejuni and campylobacter coli. these species were distinguished easily, as the corresponding melting points showed a difference of 15 degrees c. a second assay using the same biprobe and pcr conditions, but different pcr primers, was also developed to identify the less frequently encountered campylobacter fetus. ... | 2005 | 15760424 |
fly transmission of campylobacter. | an annual increase in campylobacter infection in england and wales begins in may and reaches a maximum in early june. this increase occurs in all age groups and is seen in all geographic areas. examination of risk factors that might explain this seasonal increase identifies flies as a potential source of infection. the observed pattern of infection is hypothesized to reflect an annual epidemic caused by direct or indirect contamination of people by small quantities of infected material carried b ... | 2005 | 15757548 |
could flies explain the elusive epidemiology of campylobacteriosis? | unlike salmonellosis with well-known routes of transmission, the epidemiology of campylobacteriosis is still largely unclear. known risk factors such as ingestion of contaminated food and water, direct contact with infected animals and outdoor swimming could at most only explain half the recorded cases. | 2005 | 15752427 |