enterotoxin production and serogroups of campylobacter jejuni and campylobacter coli from patients with diarrhea and from healthy laying hens.enterotoxin production, a possible virulence factor, was determined in campylobacter jejuni and campylobacter coli by two different techniques, the cho cell test and the gm1 enzyme-linked immunosorbent assay. the frequency of enterotoxigenic campylobacter strains was 32% in strains from both humans with acute enteritis and healthy laying hens, as measured by the cho cell test. the cho cell test was significantly more sensitive than the gm1 enzyme-linked immunosorbent assay in the detection of en ...19892754001
virulence of campylobacter jejuni for chicken embryos.the pathogenicity of campylobacter jejuni was examined in chicken embryos. in this system, mortality data and histopathological findings induced by organisms and by bacterium-free filtered broth were identical. the absence in chicken embryo tissues both of organisms and of an inflammatory infiltrate suggests a toxin etiology.19892754005
effects of sample holding time, temperature, and atmosphere on the isolation of campylobacter jejuni from dogs.stool specimens were collected from 39 dogs, inoculated onto campylobacter blood agar plates, and divided into four subsamples. subsamples were held at 4 and 25 degrees c in room air and in a microaerobic environment and were reinoculated at 1, 2, 3, 4, 6, and 8 h. c. jejuni survived at least 3 h when it was held at 4 degrees c, but less than 2 h when it was held at 25 degrees c. the holding atmosphere was not associated with a difference in isolation rates.19892754014
waterborne outbreak of campylobacter enteritis after outdoors infantry drill in utti, finland.diarrhoea, abdominal pain, malaise and fever affected 75 of the 88 conscript soldiers in utti, finland after an outdoors infantry drill. campylobacter jejuni, heat-stable serotype 3/43/59, was isolated from 37 out of 63 men investigated. a clear serological response was evident in the risk group and negligible in the control group. the entire population at risk was interviewed. the outbreak was associated with the consumption of untreated surface water. c. jejuni, heat-stable serotype 3/43/59, w ...19892776848
interaction of campylobacter species with antibody, complement and phagocytes.the opsonisation of four different campylobacter species for human neutrophils was studied using a chemiluminescence system and electron microscopy. opsonisation of campylobacter fetus, campylobacter coli, and campylobacter jejuni was mediated by antibody and enhanced by complement. antibody was not, however, required for the phagocytosis of campylobacter pylori because it activates the classical pathway of complement directly. this unusual property may be important in the pathogenesis of c pylo ...19892788111
in-vitro evaluation of antibiotic combinations against campylobacter fetus.we evaluated the in-vitro activity of 16 antibiotics against 22 clinical isolates of campylobacter fetus. the interaction of these antibiotics was also tested by chequer-board titration and time-kill assay. eight antibiotic combinations were evaluated. antagonism was not found with any of the combinations tested. the highest synergistic effect was obtained with a combination of ampicillin and cefazolin or ampicillin and gentamicin.19892793642
in vitro evaluation of antibiotics for treatment of meningitis caused by campylobacter fetus subspecies fetus. 19892797963
campylobacter fetus cholangitis and bacteremia. 19892804850
early treatment of campylobacter jejuni enteritis.the bacteriologic and clinical effects of early antibiotic treatment of campylobacter jejuni enteritis were studied. erythromycin rapidly eliminated c. jejuni from stools, whereas trimethoprim-sulfamethoxazole did not. despite its bacteriologic effectiveness, erythromycin did not reduce the duration or severity of diarrhea, abdominal pain, or other symptoms.19892818711
an examination of campylobacter fetus subsp. fetus by restriction endonuclease analysis and serology.restriction endonuclease analysis was used to examine 70 different strains of campylobacter fetus subsp. fetus, which were isolated from aborted sheep foetuses. the strains could be divided into seven types based on the dna fragment patterns obtained by electrophoresis after digestion with the enzyme bsteii. with one exception, digestion with the enzyme xhoi allowed the strains to be grouped identically to that for bsteii. antisera were made against formalized whole cells representative of each ...19872820112
gene transfer from escherichia coli to campylobacter species: development of shuttle vectors for genetic analysis of campylobacter jejuni.a shuttle cloning vector (pil550) has been constructed which can be mobilized from escherichia coli to campylobacter jejuni, campylobacter coli, and campylobacter fetus by complementation with the transfer functions of an incp plasmid in trans, with a frequency of 10(-4) transconjugants per donor. we also present evidence for a dna modification system in c. jejuni.19872822671
cloning and expression of a tetracycline resistance determinant from campylobacter jejuni in escherichia coli.the tetracycline resistance gene (tet) from the campylobacter jejuni plasmid pfkt1025 was cloned into both puc18 and pbr322 and was expressed when the chimeric plasmids were introduced into escherichia coli. the location of the tet determinant on the chimeric plasmids was determined by bal 31 deletion mapping within a 2.25-kilobase (kb) rsai-hincii fragment. a protein of approximately 70 kilodaltons was consistently produced by e. coli maxicells harboring the cloned tet determinant. a 500-base-p ...19872823694
gene disruption and replacement as a feasible approach for mutagenesis of campylobacter jejuni.campylobacter jejuni and campylobacter coli are important causes of human enteric infections. several determinants of pathogenicity have been proposed based on the clinical features of diarrheal disease and on the phenotypic properties of campylobacter strains. to facilitate an understanding of the genetic determinants of campylobacter virulence, we have developed a method for constructing c. jejuni mutants by shuttle mutagenesis. in the example described here, a kanamycin resistance gene was in ...19882832375
subdural empyema caused by campylobacter fetus ssp. fetus. 19862871115
identification and characterization of two campylobacter jejuni adhesins for cellular and mucous substrates.campylobacter jejuni is able to colonize the human intestinal mucosa and cause disease. for this reason, it was important to investigate mechanisms by which c. jejuni adheres to epithelial cells and intestinal mucus gel. all strains of c. jejuni used were able to adhere to int 407 epithelial cells and mucus, but high adherence to one substrate did not necessarily indicate comparable adherence to the other. the adherence of c. jejuni to cells was inhibited partially by treating the bacterial cell ...19862873103
substrate utilization by campylobacter jejuni and campylobacter attempt was made to elucidate in campylobacter spp. some of the physiologic characteristics that are reflected in the kinetics of co2 formation from four 14c-labeled substrates. campylobacter jejuni and c. coli were grown in a biphasic medium, and highly motile spiral cells were harvested at 12 h. of the media evaluated for use in the metabolic tests, minimal essential medium without glutamine, diluted with an equal volume of potassium sodium phosphate buffer (ph 7.2), provided the greatest s ...19862877615
campylobacter jejuni isolations from mexican and swedish patients, with repeated symptomatic and/or asymptomatic diarrhoea episodes.the presence of different campylobacter jejuni serotypes in swedish patients with diarrhoea and in mexican patients with or without diarrhoea was investigated with special reference to repeated isolations during the course of infection and to symptomatic and asymptomatic episodes. the study included 136 c. jejuni isolates from 62 mexican children and 173 isolates from 68 swedish patients. the bacteria were serotyped for heat-stable (hs) and heat-labile (hl) antigen. swedish patients, all with sy ...19892917617
campylobacter jejuni and campylobacter coli diarrhoea in rural and urban populations in yugoslavia.during a 4-month period during the summer of 1985, campylobacters were isolated from 338 (16.3%) of 2080 patients with acute diarrhoea attending the university hospital of infectious diseases in zagreb. of these isolates 220 (64.1%) were campylobacter jejuni and 118 (34.9%) were c. coli. the patients were drawn from three residential zones in and around zagreb: inner city, peripheral city and rural. incidences of campylobacter diarrhoea ranged from 71 per 100,000 per year in inner city residents ...19892917618
campylobacteriosis in an aborted equine fetus.abortion caused by campylobacter fetus subsp fetus was diagnosed in a 7-month-old equine fetus. the fetus was small for its gestational age. macroscopically, the proximal portion of the small intestine was hemorrhagic and its wall was thick. histologically, the brunner glands were distended with neutrophils, and the submucosa was thick, owing to fluid accumulation and/or cellular infiltrates. curved bacteria were observed in the brunner glands and intestinal glands. campylobacter fetus subsp fet ...19892917894
[meningitis caused by campylobacter fetus fetus. a case report]. 19852931701
mucus colonization as a determinant of pathogenicity in intestinal infection by campylobacter jejuni: a mouse cecal model.human isolates of the intestinal pathogen campylobacter jejuni have been shown to colonize mucus on the outer surface and deep within the intestinal crypts of gnotobiotic or germfree mice. the cecal crypts are preferentially colonized. a model of mucus colonization by c. jejuni in the mouse cecum has been developed, using antibiotic- and magnesium sulfate-treated specific-pathogen-free animals. these spiral-shaped bacteria colonize the mucus in a similar manner to the normal spiral-shaped microb ...19862935499
[campylobacter fetus ssp. fetus endocarditis]. 19862942861
[antibiotic resistance and distribution of plasmid-encoded beta- lactamases among agents of traveller's diarrhea].the polymicrobial aetiology of travellers's diarrhoea in 356 tourists travelling in thailand and burma was investigated. besides enterotoxigenic e. coli, salmonella sp. and campylobacter fetus ssp. jejuni were identified as the most important enteric pathogens. minimal inhibitory concentrations of several commonly used antibiotics were determined to reveal the percentage of enteric pathogens being resistant. 36.2% e. coli strains were found to be resistant to ampicillin and 14.3% of the campylob ...19862943094
influence of iron on growth, morphology, outer membrane protein composition, and synthesis of siderophores in campylobacter jejuni.three human isolates of campylobacter jejuni were grown in a biphasic culture medium with and without the addition of a synthetic chelator to induce iron limitation. cells grown in low-iron medium exhibited slower growth rates and altered cellular morphology. increased numbers of longer, more filamentous forms were seen in gram-stained smears. three proteins, with apparent mrs of 82,000, 76,000, and 74,000, were consistently present in the outer membrane of cells grown in low-iron medium. at lea ...19862944843
in vitro activity of a-56268 (te-031), a new macrolide, compared with that of erythromycin and clindamycin against selected gram-positive and gram-negative organisms.the in vitro activity of a-56268 was determined and compared with that of erythromycin and clindamycin against a limited spectrum of 401 gram-positive and gram-negative organisms. a-56268 was quite active against erythromycin-susceptible staphylococcus aureus, neisseria gonorrhoeae, listeria monocytogenes, streptococcus pneumoniae, streptococcus pyogenes, and group b streptococci and was moderately active against campylobacter fetus subsp. fetus. a-56268 was consistently bactericidal only for s. ...19872952063
comparative in vitro activity of a-56268 (te-031) against gram-positive and gram-negative bacteria and chlamydia trachomatis.the in vitro activity of a-56268 (te-031) was determined and compared with that of 13 antibiotics against 401 gram-positive and gram-negative bacteria and 11 strains of chlamydia trachomatis. a-56268 was very active against methicillin-susceptible staphylococcus aureus and neisseria gonorrhoeae, and was among the most active of the agents tested against listeria monocytogenes, streptococci and chlamydia trachomatis. it was moderately active against haemophilus spp., vibrio spp., campylobacter je ...19872954818
campylobacters and impaired fetal development in mice.intravenous injection of eight human strains of campylobacter fetus ss fetus and campylobacter jejuni into mice at various stages of pregnancy demonstrated significant strain differences in ability to affect implantation of the fertilised ovum and to cause resorption of the mouse fetus. implantation was significantly impaired when c. fetus ss fetus was injected intravenously on day 2 of pregnancy, but no effect was observed in mice receiving c. jejuni. on day 6 of pregnancy, before the developme ...19882961890
the effect of campylobacter lipopolysaccharide on fetal development in the mouse.purified lipopolysaccharide (lps) obtained from isolates of campylobacter fetus ss. fetus and campylobacter jejuni impaired fetal development when administered to mice on day 13 of pregnancy. strikingly more fetal resorption was produced by c. jejuni lps than by similar amounts of c. fetus ss. fetus lps. three of the four campylobacter strains examined produced lps that had no effect on maternal health, but lps from one c. jejuni strain killed all of the mice to which it was administered.19882968457
a two-year longitudinal study on the etiology of acute diarrhea in young children in northern italy.during a 24-month period, 561 young children (6 months-2 years of age) hospitalized for acute diarrhea were studied for enteric pathogens. patients positive for one or more pathogens were 359 (64.0%). infection with one pathogen was found in 266 (47.4%) patients, whereas multiple infection was detected in 93 (16.6%) patients. enteropathogens associated with disease were rotaviruses: 150 (26.7%), adenoviruses: 99 (17,6%), non-polio enteroviruses: 48 (8.5%), coronaviruses: 10 (1.8%), parvoviruses: ...19852983179
dna relatedness and biochemical features of campylobacter spp. isolated in central and south australia.investigations of the etiology of diarrhea in patients in south australia and the northern territory showed that campylobacter spp. other than campylobacter jejuni and c. coli were common in children. campylobacters which were hippurate positive, nitrate negative, and susceptible to cephalothin and polymyxins were shown to be closely related to c. jejuni by dna studies. thermotolerant catalase-negative campylobacters were also isolated. these were h2s negative and biochemically resembled the cat ...19852991331
in vitro susceptibility of campylobacter jejuni to 27 antimicrobial agents and various combinations of beta-lactams with clavulanic acid or sulbactam.the in vitro susceptibility of human isolates of campylobacter jejuni was investigated with 27 antibiotics and 8 combinations of beta-lactams with clavulanic acid or sulbactam. ansamycin, the new quinolines, erythromycin, and cefpirome were the most active drugs against c. jejuni; amoxicillin, ampicillin, cefotaxime, and ceftazidime 90% of the isolates, greater than or equal to 50 mg/liter). the activity of various beta-lactams was unchanged by the addition of clavulanic acid or sulbactam.19852994557
participation of cytochromes in some oxidation-reduction systems in campylobacter fetus.campylobacter species are rich in c-type cytochromes, including forms which bind carbon monoxide. the role of the various forms of cytochromes in campylobacter fetus has been examined in cell-free preparations by using physiological electron donor and acceptor systems. under anaerobic conditions, nadph reduced essentially all of the cytochrome c in crude cell extracts, whereas the reduction level with succinate was 50 to 60%. the carbon monoxide spectrum with nadph was predominated by the cytoch ...19852995315
plasmid-mediated tetracycline resistance in campylobacter jejuni: expression in escherichia coli and identification of homology with streptococcal class m determinant.plasmid pua466, a 45-kilobase transmissible tetracycline resistance plasmid from campylobacter jejuni was mapped with avai, avaii, bcli, hincii, psti, xhoi, and xbai. the resistance determinant was cloned and expressed in escherichia coli and was homologous with a class m determinant from streptococcus spp.19863005233
an epidemiological study of selected calf pathogens on holstein dairy farms in southwestern ontario.fecal samples from calves on 78 randomly selected holstein dairy farms in southwestern ontario were screened for salmonella, campylobacter jejuni/coli, enteropathogenic escherichia coli, rotavirus and coronavirus. based on the observed prevalence, 22% of farms had calves infected with salmonella, 13% with campylobacter jejuni/coli, 41% with enteropathogenic e. coli, 19% with rotavirus and 5% with coronavirus. these estimates can be modified, using a method developed by mullen and prost (1983) fo ...19863017528
cytotoxic and cytotonic factors produced by campylobacter jejuni, campylobacter coli, and campylobacter laridis.complete toxigenicity studies were performed on 341 strains of campylobacter spp., including 23 nonhuman isolates. toxin profiles based on both cytotonic and cytotoxic factors were determined after analyzing responses in vero, hela, cho and y-1 cells. suckling mouse assays were consistently negative for all culture filtrates tested. toxin-producing strains were frequently encountered among both the human and nonhuman strains of campylobacter jejuni, c. coli, and c. laridis investigated. strains ...19863018039
comparison between children treated at home and those requiring hospital admission for rotavirus and other enteric pathogens associated with acute diarrhea in melbourne, australia.the etiology of acute diarrhea in children less than 42 months of age attending one pediatric hospital in melbourne, australia, was studied during a 7-month period encompassing the winter of 1984. pathogens identified in 157 children treated as outpatients with mild disease were compared with those in 232 children hospitalized with severe disease. the pathogens (and frequencies among outpatients and inpatients, respectively) detected were rotaviruses (32.5 and 50.9%), enteric adenoviruses (8.9 a ...19863020082
genetic studies of kanamycin resistance in campylobacter jejuni.campylobacter jejuni 3h40 and 4b20 harbored 59-kilobase (kb) self-transmissible plasmids encoding resistance to kanamycin and tetracycline. although the two antibiotic resistances were more frequently inherited together, some transconjugants and ethidium bromide segregants which were resistant to only one of these antibiotics were recovered. the kanamycin-susceptible, tetracycline-resistant segregants carried plasmids 4 kb smaller than the 59-kb plasmids of their parents, whereas the kanamycin-r ...19863021049
characterization and expression of a cloned tetracycline resistance determinant from campylobacter jejuni plasmid pua466.a tetracycline resistance (tcr) determinant previously cloned from the campylobacter jejuni plasmid pua466 (d. e. taylor, j. bacteriol. 165:1037-1039, 1986) was localized by restriction endonuclease mapping, subcloning, and tn1000 insertion mutagenesis to a 2-kilobase region consisting of 1.8- and 0.2-kilobase hincii fragments. tcr encoded by the cloned fragment (puoa1) was expressed constitutively in escherichia coli. a protein with an apparent molecular weight of 68,000 encoded by puoa1 was pr ...19873036765
antimicrobial resistance of campylobacter jejuni and campylobacter coli with special reference to plasmid profiles of japanese clinical isolates.a total of 111 clinical isolates of campylobacter jejuni and 10 clinical isolates of campylobacter coli were characterized by their susceptibility to nine antimicrobial agents and by their plasmid profiles on agarose gel electrophoresis. all of the c. jejuni isolates were susceptible to chloramphenicol, ciprofloxacin, erythromycin, kanamycin, and nalidixic acid, but 55% were tetracycline resistant. in the 10 c. coli isolates, a high prevalence of multiple-antibiotic resistance was noted. plasmid ...19873038005
two-year survey of etiologic agents of diarrheal disease at san lazaro hospital, manila, republic of the philippines.the prevalence of bacterial pathogens and rotavirus in 2,908 patients with diarrhea who were admitted to san lazaro hospital in manila in 1983 and 1984 was determined. one or more enteric pathogens were isolated or detected in samples from 1,698 (58.4%) patients. isolation rates for the various enteropathogens were as follows: rotavirus, 30.6%; shigella spp., 11.6%; salmonella spp., 9.2%; enterotoxigenic escherichia coli (1983 only), 7.8%; vibrio cholerae biotype eltor, 3.8%; non-o1 v. cholerae, ...19873038946
colonization of gastrointestinal tracts of chicks by campylobacter jejuni.bacterial enumeration and histologic examination of organs and tissues of 8-day-old chicks 7 days after peroral inoculation with campylobacter jejuni revealed that the organism colonized primarily the lower gastrointestinal tract. the principal sites of localization were the ceca, large intestine, and cloaca, where densely packed cells of c. jejuni were observed in mucus within crypts. examination of c. jejuni-colonized crypts by transmission electron microscopy revealed that the campylobacters ...19883060015
lipopolysaccharide structures in enterobacteriaceae, pseudomonas aeruginosa, and vibrio cholerae are immunologically related to campylobacter determine whether lipopolysaccharide (lps) structures of campylobacter species are immunologically related to those of 11 other gram-negative organisms, we immunoblotted from polyacrylamide gels the lps of these strains with immune rabbit serum raised against six campylobacter jejuni strains and two campylobacter fetus strains. the lps studied were from salmonella minnesota wild type and ra to re mutants, salmonella typhi, escherichia coli, yersinia enterocolitica, vibrio cholerae, and pseudo ...19863079730
[two cases of campylobacter fetus meningitis]. 19863095453
[clinico-bacteriological study of diarrhea caused by campylobacter fetus subsp. fetus]. 19863095456
[isolation of campylobacter fetus subsp. jejuni from feces and transport media during a diarrhea epidemic]. 19853155666
[purulent pericarditis caused by campylobacter fetus ssp fetus]. 19853157173
campylobacters in man and the environment in hull and east yorkshire.campylobacter organisms isolated from water samples taken weekly from ponds and land-drains in the city of hull were compared with isolates from humans. of 314 campylobacter organisms isolated from patients, 237 (75.5%) of the strains were identified as typical campylobacter jejuni, whilst of 125 identified strains isolated from the water samples, 85 (68%) resembled c. jejuni in most respects but were hippurate hydrolysis negative by the hwang and ederer method. the ponds and land drains in the ...19883181312
isolation of campylobacter jejuni from the bile of a cholecystic patient.described is the isolation of campylobacter jejuni from the bile of a cholecystic patient admitted for abdominal pain to an intensive care unit. a cholecystectomy was performed, and the patient responded to erythromycin therapy. details of this uncommon isolation are given.19883183005
infection with campylobacter coli and campylobacter fetus in a patient on continuous ambulatory peritoneal dialysis. 19883183412
detection of two different kanamycin resistance genes in naturally occurring isolates of campylobacter jejuni and campylobacter coli.a total of 225 isolates of campylobacter jejuni and 54 isolates of campylobacter coli were screened for resistance to kanamycin. among these, five resistant isolates of c. jejuni and six resistant isolates of c. coli, all with different plasmid patterns, were identified. each contained at least one plasmid greater than or equal to 41 kilobases in size. the mic of kanamycin for all 11 strains was determined to be greater than or equal to 256 micrograms/ml by an agar dilution method. in addition, ...19883190204
is enrichment culture necessary for isolating campylobacter jejuni from faeces? 19883192739
nosocomial meningitis due to campylobacter fetus subsp. fetus in a neonatal intensive care unit. 19883197739
trichomoniasis and campylobacteriosis in bulls in the republic of transkei.trichomonas foetus was demonstrated in 23/87 (26.4%) and campylobacter fetus in 25/87 (28.7%) of bulls tested in transkei. a total of 16/87 (18.39%) of bulls tested were positive for both trichomonas foetus and campylobacter fetus. bulls from 14 sites in transkei were tested and trichomonas foetus was isolated at 9 of these sites. campylobacter fetus was isolated at 10 of the 14 sites. the results indicate that both trichomonas foetus and campylobacter fetus are widespread throughout the cattle ...19883199409
role of the 92.5-kilodalton outer membrane protein of campylobacter jejuni in serological reactions.outer membrane proteins from 12 strains of campylobacter jejuni were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting with rabbit antisera prepared against formalin-treated cell suspensions of three of the strains. the 92.5-kilodalton protein was confirmed to be a minor outer membrane protein and exhibited serological heterogeneity. serological specificities of the proteins, however, were found not to correlate with serotypic specificities of the strains, ...19883230126
paediatric campylobacter diarrhoea from household exposure to live chickens in lima, peru.although campylobacter jejuni is a frequent enteropathogen in cases of paediatric diarrhoea in developing countries, its route of transmission is not well understood. an age-matched, case-control study of children with c. jejuni diarrhoea was therefore carried out in lima, peru, from january 1983 to april 1986 to identify the risk factors and vehicles of transmissions. as cases, 104 children less than 3 years of age were selected and compared with controls of the same age with non-gastrointestin ...19883262442
high-voltage electroporation of bacteria: genetic transformation of campylobacter jejuni with plasmid dna.electroporation permits the uptake of dna by mammalian cells and plant protoplasts because it induces transient permeability of the cell membrane. we investigated the utility of high-voltage electroporation as a method for genetic transformation of intact bacterial cells by using the enteric pathogen campylobacter jejuni as a model system. this report demonstrates that the application of high-voltage discharges to bacterial cells permits genetic transformation. our method involves exposure of a ...19883277182
campylobacter fetus endocarditis on a prosthetic heart valve.campylobacter fetus infection in man is rare. few cases of campylobacter endocarditis have been described, and none have been associated with a prosthetic heart valve. we have reported a case of campylobacter fetus ssp fetus on a prosthetic mitral valve in a 48-year-old woman who was treated successfully with penicillin and streptomycin (and later with gentamicin). replacement of the prosthetic valve was done because of stenosis and vegetations. pathologic examination showed vegetations on a cal ...19883287646
acid and alkaline campylobacter extracts in the demonstration of antibody response in hyperimmunized rabbits.acid and alkaline extracts from several campylobacter strains were used as antigens in enzyme immunoassay (eia). antibodies were detected in rabbits immunized with formalin-treated, boiled, or autoclaved whole bacteria of different campylobacter subspecies. for the preparation of the extracts, three campylobacter fetus subsp. venerealis strains, three campylobacter fetus subsp. fetus strains, four campylobacter coli, and five campylobacter jejuni strains were used. the acid extracts of campyloba ...19873296648
multiple enteric pathogens in a patient with diarrhoea.a woman who recently arrived in canada from india developed a febrile, diarrhoeal illness. stool culture revealed the presence of salmonella typhi, campylobacter fetus subspecies jejuni and shigella flexneri. concurrent infection with these three pathogens does not appear to have been previously reported. the possibility of multiple pathogens should be considered in travellers who fail to respond to treatment of a single organism.19873299934
structural and antigenic properties of lipopolysaccharides from serotype reference strains of campylobacter investigate the molecular basis for heat-stable antigenic diversity in campylobacter jejuni, lipopolysaccharides (lpss) from serotype reference strains and serotyped isolates were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis coupled with silver staining and immunoblotting. by silver staining, only low-mr components, consisting of one major band and as many as three minor bands ranging in mr from 4,500 to 5,000, were detected. however, by immunoblotting with homologous ...19873301678
expression of campylobacter jejuni invasiveness in cell cultures coinfected with other bacteria.enteroinvasive salmonella, shigella, and escherichia coli strains were found to exert an effect which rendered campylobacter jejuni capable of intracellular localization in epithelial cells in vitro. when monolayers of hep-2 or a-549 cells were challenged with pure cultures of c. jejuni or campylobacter coli, none of the eight strains tested invaded the cells. in contrast, four of these strains were able to localize intracellularly when the cells were challenged with a mixture of campylobacters ...19873312015
meningitis due to campylobacter fetus intestinalis in a kidney transplant recipient. a case report.a 47-year-old man developed campylobacter fetus intestinalis meningitis 3 years after cadaver renal transplantation. the infection was successfully controlled with chloramphenicol followed by erythromycin. there were no relapses during the past 5 years and the allograft function remains normal. the available evidence suggests that the campylobacter sepsis was associated with nutritional therapy, the patient received in a mexican clinic. the present case is reported because of its clinical import ...19873324765
a comparative study of the growth of campylobacter fetus strains in liquid media.the growth of c. fetus fetus, c. fetus venerealis and c. fetus venerealis biotype intermedius was examined in 10 liquid media. from the data obtained, a 10% inoculum size and an oxygen level of 6% seemed imperative for consistent growth, especially for the c. fetus venerealis strain. a lowered redox potential obtained by the addition of 0, 1% cysteine-hc1 to the media was stimulatory. the medium which yielded the best growth was the one described by dennis & jones (1959). the fastidious c. fetus ...19873328136
mucosal and systemic immunity to campylobacter jejuni in rabbits after gastric inoculation.the mucosal and systemic immune responses to campylobacter jejuni were studied in rabbits receiving gastric inoculation with live organisms. a lavage procedure was used to facilitate repeated monitoring of the intestinal immune response to c. jejuni. immunity to c. jejuni was determined by secondary challenge by using the removable intestinal tie adult rabbit diarrhea (ritard) model and monitoring for resistance to colonization and bacteremia. oral-gastric inoculation of normal rabbits produced ...19883335413
the extent of surface contamination of retailed chickens with campylobacter jejuni serogroups.eighty-two chickens purchased at 11 retailers (supplied by 12 wholesalers) in the south of england were cultured for campylobacter jejuni by a method involving total immersion. the organism was isolated from 22 (48%) of 46 fresh birds, 12 of 12 uneviscerated (new york dressed) birds, but only 1 of 24 frozen birds. viable counts of up to 1.5 x 10(6)/chicken were obtained from fresh birds and 2.4 x 10(7)/chicken from uneviscerated birds. surface swabbing of breasts, thighs, wings and vents of fres ...19883338503
experimental colonization of broiler chicks with campylobacter jejuni.minimal colonization inocula for two broiler strains of campylobacter jejuni were determined in broiler chicks aged 2-3 days and 2 weeks. individually housed chicks were exposed to a single oral or cloacal challenge. diarrhoeal symptoms were absent in all 380 chicks included in the study. chick susceptibility to the two c. jejuni strains varied. colonization was effected by less than 10(2)-10(4) colony forming units (c.f.u.) via cloacal challenge and 10(4)-10(6) c.f.u. via the oral route. coloni ...19883338504
enteropathogens associated with acute infantile diarrhoea in cape town.the results of a year-long controlled study on the causation of acute infectious diarrhoea in infants are reported. campylobacter fetus subspecies jejuni, rotavirus and certain enteropathogenic escherichia coli (epec) types were identified as the most common enteropathogens. shigella, salmonella group b and yersinia enterocolitica were significant but less common pathogens. campylobacter, rotavirus, the significant epec types and shigella infections were more frequent in the warm summer and autu ...19883340928
characterization of an unclassified microaerophilic bacterium associated with gastroenteritis.four isolates of an unclassified microaerophilic bacterium resembling campylobacter species were characterized by growth requirements, microscopic examination, biochemical characteristics, antimicrobial susceptibility tests, and protein profile analysis. the unclassified isolates were differentiated from campylobacter jejuni, campylobacter coli, campylobacter fetus subsp. fetus, campylobacter laridis, campylobacter pylori, and an ovine isolate. the bacterium was fusiform shaped with a corrugated ...19883343302
evaluation of the campyslide agglutination test for confirmatory identification of selected campylobacter species.the utility of a rapid latex slide agglutination test (campyslide; bbl microbiology systems, cockeysville, md.) in detecting selected campylobacter spp. was evaluated and compared with that of conventional identification methods. isolated colonies suggestive of campylobacter spp. were tested directly from primary selective media after incubation at 42 degrees c under microaerophilic conditions. stock cultures of campylobacter jejuni (n = 27) and c. coli (n = 3) were correctly confirmed to the ge ...19883343315
infertility in cattle in south-west scotland caused by an 'intermediate' strain of campylobacter fetus subspecies fetus (formerly campylobacter fetus intestinalis). 19883345413
concurrent bovine virus diarrhoea virus and campylobacter fetus infection in an aborted bovine fetus. 19883354166
characterization of freshly isolated campylobacter coli strains and suitability of selective media for their growth.typical and atypical campylobacter strains were isolated from the colons of cattle and swine by techniques that enabled the selective pressures of antibiotics to be avoided. some cephalothin-susceptible strains and a strain with an indeterminate hippurate reaction were classified as campylobacter coli by dna homology testing. tetracycline-resistant isolates were obtained from animals with no recorded exposure to antibiotics. a selection of 12 c. coli and 6 c. jejuni strains was used to determine ...19883356790
campylobacter jejuni antibodies in nigerian children.titers of complement-fixing (cf) antibody to campylobacter jejuni were demonstrated in 87 (36.7%) of 237 infants 6 to 15 months old in jos, nigeria. of the total number of children examined, 81 had acute diarrhea and 27 of them (33.3%) were found to have cf antibodies in their serum. the remaining 156 children were asymptomatic, and 60 (38.4%) of them had cf antibodies. in the diarrheal group, 27 of 75 children 6 to 8 months old were cf antibody positive. there was no significant difference in t ...19883356797
purification and characterization of a family of high molecular weight surface-array proteins from campylobacter fetus.a variety of gram-negative and gram-positive bacteria possess crystalline surface layers, although little is known of their function. we previously have shown that the high molecular weight surface-array proteins of campylobacter fetus are important in both the pathogenicity and antigenicity of this organism. for biochemical and immunological characterization, we purified high molecular weight (100,000, 127,000, 149,000) surface-array proteins from three c. fetus strains using sequential gel fil ...19883360785
pathogenesis of campylobacter fetus infections. failure of encapsulated campylobacter fetus to bind c3b explains serum and phagocytosis resistance.campylobacter fetus ssp. fetus strains causing systemic infections in humans are highly resistant to normal and immune serum, which is due to the presence of high molecular weight (100,000, 127,000, or 149,000) surface (s-layer) proteins. using serum-resistant parental strains (82-40 lp and 23d) containing the 100,000-mol wt protein and serum-sensitive mutants (82-40 hp and 23b) differing only in that they lack the 100,000-mol wt protein capsule, we examined complement binding and activation, an ...19883366901
cryopreservation studies of strains of campylobacter fetus ss. fetus, one of campylobacter fetus ss. venerealis, and one of campylobacter jejuni were preserved using a variety of cryopreservation methods. organisms were frozen to -150 degrees c in a liquid nitrogen refrigerator, in the freezer compartment of a refrigerator (-20 degrees c), and in a mechanical freezer (-65 degrees c). in the latter two cases, viabilities of the organisms were compared after being frozen in brucella albimi broth and 10% glycerol. viabi ...19883371059
chemotactic behavior of campylobacter jejuni.the chemotactic behavior of campylobacter jejuni was determined in the presence of different amino acids, carbohydrates, organic acids, and preparations and constituents of mucin and bile. l-fucose was the only carbohydrate and l-aspartate, l-cysteine, l-glutamate, and l-serine were the only amino acids producing a chemotactic (positive) response. several salts of organic acids, including pyruvate, succinate, fumarate, citrate, malate, and alpha-ketoglutarate, were also chemoattractants, as were ...19883372020
the immune system response to campylobacter infection.campylobacter may be one of the most common causes of bacterial gastroenteritis (ge) in children. it has recently been suggested that it is one of the bacterial pathogens most likely to infect immune-compromised children, and it may facilitate colonization of enteric pathogens. the immune system response was studied in 12 children with campylobacter fetus subspecies jejuni (cbj) infections. serum concentrations of iga, igm, and igg were analyzed using a beckman auto-analyzer. sera specific ab to ...19883374406
small-scale dna preparation for rapid genetic identification of campylobacter species without radioisotope.a simplified and rapid genetic identification method for campylobacter species without radioisotope was established. three different amounts of dna (200, 50, and 12.5 ng) extracted from each type strain of campylobacter species with standard marmur's procedure were spotted on a nitrocellulose filter. dna obtained from one ml bacterial suspension at a concentration of mcfarland standard turbidity no. 1 of campylobacter fetus, c. jejuni, c. coli, and c. pylori isolates were sufficiently labeled wi ...19883374408
vaccination of pregnant guinea pigs with campylobacter fetus: effects of antigen dose, campylobacter strain, and adjuvant type.for the purpose of developing and improving vaccines to protect cattle against bovine genital campylobacteriosis, the interaction of vaccine dose, strain(s) of campylobacter fetus, and type of adjuvant was studied in the pregnant guinea pig model. five strains of c fetus were used in the preparation of 20 monovalent vaccines containing various doses of bacteria (0.1 mg to 20 mg, dry weight) in freund incomplete adjuvant, freund complete adjuvant, or aluminum hydroxide gel adjuvant. vaccinal prot ...19883377303
improved blood free selective medium for isolating campylobacter jejuni from faecal specimens. 19883385002
structural and biochemical analyses of a surface array protein of campylobacter fetus.electron microscopic examination of ultrathin sections and freeze-etched and shadow cast preparations of a bovine prepuce isolate of campylobacter fetus vc119 showed an s layer with subunits in an apparent linear arrangement. surface radioiodination, enzyme digestion, low-ph extraction, and western immunoblotting showed that the layer was composed mainly of one protein which is the predominant protein antigen of c. fetus. this protein was purified to homogeneity by gel filtration, ion-exchange c ...19883410826
laboratory and clinical evaluation of isolation media for campylobacter jejuni.six selective isolation media were evaluated for their ability to support the growth of campylobacter jejuni. colony counts of 70 isolated strains of c. jejuni and recovery studies on these strains in simulated positive feces samples demonstrated that bolton and hutchinson' charcoal, cefoperazone, deoxycholate agar and karmali's charcoal-based selective medium produced the highest recovery rates with the greatest suppression of other fecal flora. c. jejuni colonies were more easily recognized on ...19873429621
bovine abortion due to campylobacter fetus. 19873439950
the demonstration of the k-antigen of campylobacter fetus venerealis using a microtitre agglutination test.using specific antisera prepared against heat-treated and untreated cells in a microtitre system, 7 strains of campylobacter fetus venerealis were examined for the presence of k-antigen. the technique was effective for distinguishing between strains and it was found that 4 out of 7 isolates contained microcapsules, therefore being potentially suitable for vaccine production.19873444618
lipopolysaccharide structures of campylobacter fetus are related to heat-stable determine whether lipopolysaccharide (lps) structures of campylobacter fetus are related to the three known heat-stable serogroups, proteinase k-treated whole cell lysates obtained from strains of each serogroup were electrophoresed in polyacrylamide gels. all strains had smooth-type lps with multiple high-molecular-weight repeating units. the profiles of serogroup a from c. fetus subsp. fetus and from c. fetus subsp. venerealis were identical, but they were different from those of c. fetus s ...19863510169
two-dimensional thin-layer chromatography for the specific detection of hippurate hydrolysis by microorganisms.glycine, one of the end products of hippurate hydrolysis by microorganisms, was detected by a rapid, specific technique utilizing two-dimensional thin-layer chromatography. a loopful of growth of each organism from its suitable agar medium was washed, suspended, and incubated with 0.1% sodium hippurate for 30 min at 37 degrees c. the supernatant of the incubated suspension from each organism was then dansylated, and the dansyl derivatives were separated by two-dimensional thin-layer chromatograp ...19863517036
enrichment for detection of campylobacter jejuni.gifu anaerobe-modified semisolid medium supplemented with vancomycin, polymyxin b, trimethoprim, and amphotericin b can serve as an efficient enrichment for the recovery of campylobacter jejuni under aerobic conditions. it is superior to the direct plating method in its recovery of very small numbers of c. jejuni. blood supplementation, agitation, and elevated temperatures are not required for this improved recovery.19863519661
antigenicity of campylobacter jejuni flagella.we studied the antigenicity of a wild-type flagellate and motile (f+m+) campylobacter jejuni strain (81116) and two daughter mutants, one flagellate and immotile (f+m-) and one aflagellate and immotile (f-m-). by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of acid-extracted surface proteins, a 63-kilodalton (kda) band identified from sheared flagella as the flagellar protein was present in the f+m+ and f+m- strains but not in the f-m- strain. no other differences in protein profile ...19863522430
abortion and perinatal sepsis associated with campylobacter infection.fetal loss or neonatal sepsis associated with campylobacter infection during pregnancy is infrequently recognized. as reported herein, one case of premature labor and neonatal sepsis due to campylobacter fetus subspecies fetus was treated successfully with ampicillin and gentamicin. only 19 similar cases have been cited in the literature. a review of these 19 cases reveals that the campylobacter species involved were probably c. fetus subspecies fetus in nine instances, campylobacter jejuni in n ...19863523697
pathogenesis of campylobacter spp. in athymic and euthymic germfree athymic (nu/nu) and euthymic (+/nu) germfree balb/c mice were orally challenged with pure cultures of campylobacter jejuni (human clinical fecal strains) and a human blood isolate of campylobacter fetus subsp. fetus. after a period of adaptation to the mouse intestinal tract, all three c. jejuni strains caused disease in gnotobiotic mice. mouse-adapted, weakly cytotoxic c. jejuni 45100 consistently induced disease symptoms (transient diarrhea, cecal shrinkage, and acute inflammatory change ...19863525411
direct immunofluorescence microscopy for rapid screening of campylobacter enteritis.diagnostic use of a direct fluorescent-antibody test for detection of campylobacter jejuni and c. coli in human fecal specimens (n = 497) was compared with detection by culturing (specificity, 99.7%; sensitivity, 40%). conjugates were prepared from immunoglobulin g antibody against 22 lior c. jejuni and c. coli reference strains (h. lior, d. l. woodward, j. a. edgar, l. j. laroche, and p. gill, j. clin. microbiol. 15:761-768, 1982). interestingly, the serotypes of cultures tested by the direct f ...19863533990
susceptibility of campylobacter jejuni and yersinia enterocolitica to uv radiation.two enteric pathogens, campylobacter jejuni and yersinia enterocolitica serogroup o:3, together with escherichia coli, were investigated for susceptibility to uv radiation at 254 nm. the uv dose required for a 3-log reduction (99.9% inactivation) of c. jejuni, y. enterocolitica, and e. coli was 1.8, 2.7, and 5.0 mws/cm2, respectively. using e. coli as the basis for comparison, it appears that c. jejuni and y. enterocolitica serogroup o:3 are more sensitive to uv than many of the pathogens associ ...19873551844
variations in the virulence, for pregnant guinea pigs, of campylobacters isolated from man.pregnant guinea pigs were used to compare the virulence of four human isolates of campylobacter fetus ss. fetus and four of c. jejuni on the basis of their ability to cause abortion and bacteraemia. of the four strains of c. fetus ss. fetus two produced abortion readily after intramuscular injection. the four c. jejuni isolates were, however, of comparatively low virulence and no differences between them were demonstrated. some of the isolates differed in their ability to survive in vitro in hum ...19873560189
[infections caused by campylobacter fetus]. 19873561577
intestinal mucus gel and secretory antibody are barriers to campylobacter jejuni adherence to int 407 in vitro mucus assay was developed to study the role of mucus gel and secretory immunoglobulin a (siga) in preventing attachment of campylobacter jejuni to int 407 cells. an overlay of rabbit small intestinal mucus was found to impede the attachment of c. jejuni to a monolayer of int 407 cells. mucus from rabbits previously colonized with c. jejuni was found to completely inhibit bacterial adherence to the underlying cells. anti-campylobacter siga was readily detected in mucus samples from pr ...19873570473
campylobacter-associated appendicitis: prevalence and clinicopathologic determine the prevalence of campylobacter fetus subspecies jejuni-associated appendicitis, we studied, retrospectively, by means of immunohistochemistry, the appendectomy specimens of 116 consecutive patients, operated upon because of suspected acute appendicitis. we found immunohistochemical evidence of campylobacter fetus subspecies jejuni infection in three patients. these findings were confirmed by electron microscopy. based upon these three cases and five additional appendectomy specimen ...19873574983
seasonal occurrence of campylobacter spp. in surface waters and their correlation with standard indicator bacteria.campylobacter jejuni, campylobacter coli, and a campylobacter-like organism were isolated from a number of natural water sources in central washington, including ponds, lakes, and small mountain streams at elevations ranging from 1,460 to 5,400 feet (ca. 445 to 1,646 m) above sea level. at the two sites where extensive sampling was done, the bacteria were recovered throughout the year. generally, the recovery rates were highest in the fall and winter months and lowest during the spring and summe ...19873579268
campylobacter jejuni in dairy cows and raw milk.twelve herds of dairy cows were examined by rectal swabbing for the presence of campylobacter jejuni. ten herds were positive with the incidence of colonized animals ranging from 10 to 72% of those tested. with the exception of the two negative herds where mains water only was consumed, all animals drank from rivers or streams when grazing. there was no relationship between total and coliform counts and the presence of c. jejuni in raw milk. however, milk from one farm that consistently gave pos ...19873595744
[right-sided endocarditis caused by campylobacter fetus]. 19863602699
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