clinical evaluation of the efficacy of inoculating cattle with a vaccine containing tritrichomonas test the efficacy of a polyvalent tritrichomonas foetus vaccine, 130 nulliparous heifers were randomly assigned to either receive the test t foetus vaccine or to serve as nonvaccinated controls. the polyvalent test vaccine consisted of a campylobacter fetus/leptospira canicola-grippotyphosa-hardjo-icterohaemorrhagiae-pamona bacterine containing 5 x 10(7) killed t foetus/dose. the polyvalent control vaccine consisted of the aforementioned formulation without t foetus. heifers were administered ...19921466496
pyogenic arthritis of the hip due to campylobacter fetus--a case report.septic arthritis of the hip caused by campylobacter fetus subsp. fetusis very rare. the authoris isolated c. fetus subsp. fetus from a specimen of the left hip. the patient was a 53-year old man with a history of heavy drinking, diabetes, and chronic hepatitis, and had been suffering from avascular necrosis of both femoral heads. it was considered that the organism invaded already damaged tissue of the joint. the patient was treated with intravenous antibiotics and later received successful tota ...19921502834
[human campylobacteriosis caused by campylobacter fetus]. 19921507827
[acute aortic insufficiency following endocarditis due to infection with campylobacter fetus subspecies fetus].a 50-year-old male alcoholic addict, examined because of diarrhoea with fever was found to have campylobacter jejuni in blood and stool cultures. after administration of broad-spectrum penicillin all acute symptoms disappeared but he lost 8 kg within 3 months and his general state health gradually deteriorated. after 3 months he suddenly developed leg oedema, dyspnoea and bouts of fever up to 38.8 degrees c. a loud cardiac murmur was now heard. echocardiography demonstrated vegetations on the re ...19921516529
characterization of the campylobacter fetus sapa promoter: evidence that the sapa promoter is deleted in spontaneous mutant strains.wild-type campylobacter fetus cells possess s-layer proteins (s+ phenotype), whereas after laboratory passage, spontaneous stable mutants that do not express these proteins (s- phenotype) arise. to determine the molecular mechanisms by which c. fetus changes to the s- phenotype, we studied wild-type strain 23d, from which the sapa gene encoding the 97-kda s-layer protein has been cloned, and strain 23b, a spontaneous s- mutant. we compared these strains with another pair of strains, lp (s+) and ...19921522068
neonatal campylobacter fetus meningitis: a report of an unusual case.campylobacter fetus is a rare cause of meningitis in the pediatric age group and, in particular, among neonates. the clinical presentation of campylobacter meningitis in high-risk neonates is not well-described. a review of campylobacter meningitis by lee et al in 1985 reported nine cases occurring in neonates, of which only one case was caused by c. fetus. in the ensuing six years only three more confirmed cases of neonatal meningitis caused by c. fetus have been reported. we here report anothe ...19921563204
[campylobacter fetus spondylodiscitis].the case of a sixty-two year old man with campylobacter fetus spondylodiscitis is reported. despite of adequate antibiotherapy, the outcome was bad and death occurred. three other cases of campylobacter fetus spondylodiscitis are reported in the literature. tomodensitometry disclosed a paravertebral abscess with gas, that make our observation original.19921579850
antigenic differences among campylobacter fetus s-layer proteins.sodium dodecyl sulfate-polyacrylamide gel electrophoresis of s-layer proteins extracted from campylobacter fetus strains by using acid glycine buffer showed that the predominant s-layer proteins of different strains had subunit molecular weights in the range of 90,000 to 140,000. electron microscopy revealed oblique s-layer lattices with a spacing of approximately 5.6 nm (gamma = 75 degrees) on wild-type strains vc1, vc119, vc202, and vc203. three variants of c. fetus vc119 producing a predomina ...19901697574
revision of campylobacter, helicobacter, and wolinella taxonomy: emendation of generic descriptions and proposal of arcobacter gen. nov.hybridization experiments were carried out between dnas from more than 70 strains of campylobacter spp. and related taxa and either 3h-labeled 23s rrnas from reference strains belonging to campylobacter fetus, campylobacter concisus, campylobacter sputorum, campylobacter coli, and campylobacter nitrofigilis, an unnamed campylobacter sp. strain, and a wolinella succinogenes strain or 3h- or 14c-labeled 23s rrnas from 13 gram-negative reference strains. an immunotyping analysis of 130 antigens ver ...19911704793
oligodeoxynucleotide probes for campylobacter fetus and campylobacter hyointestinalis based on 16s rrna sequences.deoxyoligonucleotide probes were constructed for the identification of campylobacter fetus and campylobacter hyointestinalis based on 16s rrna sequence data. probes were targeted to hypervariable regions of 16s rrna. specificity of oligonucleotide probes was tested in a colony blot assay with type strains of 15 campylobacter and arcobacter species as well as in a slot blot format using genomic dna extracted from field strains of c. fetus and c. hyointestinalis. two oligonucleotides were construc ...19911723076
reattachment of surface array proteins to campylobacter fetus cells.campylobacter fetus strains may be of serotype a or b, a property associated with lipopolysaccharide (lps) structure. wild-type c. fetus strains contain surface array proteins (s-layer proteins) that may be extracted in water and that are critical for virulence. to explore the relationship of s-layer proteins to other surface components, we reattached s-layer proteins onto s- template cells generated by spontaneous mutation or by serial extractions of s+ cells with water. reattachment occurred i ...19921735716
characterization of an immunoreactive species-specific 19-kilodalton outer membrane protein from helicobacter pylori by using a monoclonal antibody.immunoblotting experiments on hyperimmune rabbit serum and sera from patients with helicobacter pylori gastritis showed a consistent antibody response to a 19-kda outer membrane protein antigen. a monoclonal antibody, designated hp 40, which reacted by western immunoblotting with this protein was produced. it was shared by all h. pylori strains tested (d 273, nctc 11637, and 24 wild strains) but not by the thermophilic campylobacter species, campylobacter fetus, helicobacter mustellae, or helico ...19911761682
campylobacter fetus subspecies fetus infection.during a six-year period five patients with campylobacter fetus subspecies fetus infections were seen at the mayo clinic in rochester, minnesota. bacteremia was observed in two patients, one presenting with aortic valve endocarditis and the other with abdominal atherosclerotic aortic aneurysm. c. fetus subsp. fetus was isolated from tibial tissue of a patient with osteomyelitis. diarrhea was the main complaint of two further patients, and was also mentioned by the patient with the aortic aneurys ...19911762387
prevalence of bovine campylobacteriosis in indigenous cattle of three states in nigeria.a survey of bovine campylobacteriosis in breeding bulls and cows was carried out in the states of kaduna, kano and borno. six hundred and eighty nine cattle composed of 585 and 104 breeding bulls and cows respectively were sampled. campylobacter fetus subsp. venerealis was isolated from 12 bulls while campylobacter fetus subsp. fetus was isolated from three of them. campylobacter fetus subsp. fetus was isolated from four cows while campylobacter fetus subsp. veneralis was isolated from one cow. ...19911763437
comparison of surface antigens of some campylobacter fetus subsp. fetus strains of ovine origin by polyacrylamide gel electrophoresis and immunoblotting.sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and immunoblotting were used to identify and to compare the surface antigens of eight c. fetus subsp. fetus strains. seven strains (one of serogroup a and six of serogroup b) were isolated from aborted ovine fetuses, while one strain (serogroup a) originated from an aborted calf fetus. saline extracts at 56 degrees c and 100 degrees c were used as antigens. antisera were produced in rabbits. in saline extracts (56 degrees c) of ...19911776377
campylobacter spondylodiscitis and deficiency of cellular immunity.we describe a case of spondylodiscitis caused by campylobacter fetus in a previously healthy patient. this is the first report of this agent causing discovertebral infection, which generated cellular immune deficiency, reversible with the cure.19911795335
[bacteremia from campylobacter fetus. increasing interest and incidence].three bacteremias of campylobacter fetus were described. one was a male patient and two were females. underlying illnesses were present in all of them (hodgkin disease, aids and hepatic cirrhosis respectively). they were all admitted because of fever and no other symptoms of infectious focus were present. physical findings were not relevant. the blood cultures became positive in days 6, 7 and 9 respectively. antibiotic treatments were not standardised, so no conclusions can be drawn. the evoluti ...19911943296
diagnosis by elisa of bovine abortion due to campylobacter enzyme-linked immunosorbent assay (elisa) was developed to detect antigen-specific secretory iga antibody in bovine vaginal mucus after abortion due to campylobacter fetus subsp venerealis. abortions were diagnosed by isolating the organism from 8 foetuses and/or foetal membranes and by histopathology. vaginal mucus was collected from 7 cows shortly after abortion. all showed a high level of iga antibody in their vaginal mucus when they were compared with an uninfected control group. the new ...19911953551
multivariate analyses of cellular fatty acids in bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp.the genera bacteroides, wolinella, and campylobacter contain several similar species that require taxonomic revision. fatty acid profiles of whole bacterial cells have proven useful for taxonomy. in this study, cellular fatty acids from bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp. were identified and quantitated by gas chromatography and gas chromatography-mass spectrometry, and the data were subjected to principal component analyses. bacteroides fragilis, the type s ...19911993755
freeze-substitution of gram-negative eubacteria: general cell morphology and envelope profiles.freeze-substitution was performed on strains of escherichia coli, pasteurella multocida, campylobacter fetus, vibrio cholerae, pseudomonas aeruginosa, pseudomonas putida, aeromonas salmonicida, proteus mirabilis, haemophilus pleuropneumoniae, caulobacter crescentus, and leptothrix discophora with a substitution medium composed of 2% osmium tetroxide and 2% uranyl acetate in anhydrous acetone. a thick periplasmic gel ranging from 10.6 to 14.3 nm in width was displayed in e. coli k-12, k30, and hi ...19911999383
[campylobacter fetus ssp fetus septicemia associated with a colonic abscess].campylobacter is considered to be an opportunistic agent. the authors relate an unusual case with campylobacter fetus ssp fetus (cf) septicemia and colic abscess. human campylobacteriosis is presumed to be a food-born disease related to contaminated animal products such as milk or meat. in some cases cf may be transmitted by drinking water or by fecal soiling via the hands. conventional treatment uses macrolides with decrease the duration of diarrhea and reduce the fecal excretion of cf. macroli ...19912029182
correlation between molecular size of the surface array protein and morphology and antigenicity of the campylobacter fetus s layer.the correlation between the molecular size of the surface layer protein (s protein) and both structure and antigenicity of the campylobacter fetus surface layer (s layer) was investigated in several clinical strains and their spontaneous variants which produce s proteins of molecular weights (mw) different from those of the parents. only three molecular sizes of the s proteins were observed (98, 127, and 149 kda) in the parental and variant strains. immunologically, the 98-kda protein and the 14 ...19912037362
[endocarditis caused by campylobacter fetus]. 19912051801
campylobacter fetus subspecies fetus meningitis with chronic alcoholism and diabetes mellitus.a case of campylobacter fetus subspecies fetus meningitis is reported. the patient had underlying diseases, namely chronic alcoholism and diabetes mellitus. the infection did not respond to piperacillin and cefotaxime, but did respond to ampicillin and moxalactam. the patient was discharged on the 33rd hospital day showing no neurological deficit complications, and has remained free of recurrent disease for one month after the discontinuation of therapy.19902089181
[bacteremia caused by campylobacter fetus ssp fetus in a patient with liver cirrhosis]. 19902090246
[bacteremia caused by campylobacter fetus ssp fetus in an hiv-positive patient with liver cirrhosis]. 19902090247
adherence of helicobacter pylori cells and their surface components to hela cell membranes.four helicobacter pylori strains were used to develop in vitro methods to assess adherence to hela cells. using direct detection by microscopy, adhesion scores increased with the initial bacteria-to-cell ratio. the urease method assessed h. pylori bound to hela cells by their urease activity. the percentage of the original inoculum adhering to hela cells remained constant for initial ratios from 10(2) to 10(5) bacteria per cell. an elisa using anti-h. pylori serum assessed whole bacteria or comp ...19902097496
[pericarditis caused by campylobacter fetus fetus]. 19902099869
microbiological quality and safety of zoo food.two types of commercial products for feeding zoo animals (a frozen meat product, referred to as zoo food, and a dry product, referred to as dry food) were microbiologically examined for spoilage organisms (aerobic, psychrotrophic, coliform, escherichia coli, mold, and yeasts) and pathogens (salmonella spp., listeria monocytogenes, and campylobacter jejuni). levels of microorganisms in frozen ground zoo food were compared with those in frozen ground beef and frozen ground turkey meat. the level o ...19902111115
hydrophobic characterisation of helicobacter (campylobacter) pylori.cell-surface hydrophobicity of helicobacter (formerly campylobacter) pylori was tested by aqueous two-phase partitioning and hydrophobic interaction chromatography. the hydrophobicity of h. pylori greatly exceeded that of campylobacter fetus subsp. fetus, c. jejuni and bacillus subtilis. a partition coefficient (pc) of hydrophobicity in the two-phase system was determined for h. pylori. pc was dependent on ph and the pc value was increased by greater than 20-fold at ph 2.5. lithium cations incre ...19902113100
identification of campylobacter jejuni surface proteins that bind to eucaryotic cells in understand the role of campylobacter jejuni surface proteins in the interaction of c. jejuni with cultured mammalian cell lines in vitro, we developed a ligand-binding assay. this procedure allowed us to antigenically identify c. jejuni outer membrane proteins (omps) that attach to intact host cell membranes. omps isolated from an invasive strain and a less invasive strain were antigenically indistinguishable. however, we found that proteins with molecular masses of 28 and 32 kilodaltons (kda ...19902160431
use of pulsed-field agarose gel electrophoresis to size genomes of campylobacter species and to construct a sali map of campylobacter jejuni determine the physical length of the chromosome of campylobacter jejuni, the genome was subjected to digestion by a series of restriction endonucleases to produce a small number of large restriction fragments. these fragments were then separated by pulsed-field gel electrophoresis with the contour-clamped homogeneous electric field system. the dna of c. jejuni, with its low g+c content, was found to have no restriction sites for enzymes noti and sfii, which cut a high-g+c regions. most of the ...19902168376
cardiovascular and bacteremic manifestations of campylobacter fetus infection: case report and review.a case of bacteremia due to campylobacter fetus subspecies fetus with concomitant pleuropericarditis in a previously healthy patient is presented. the organism is ubiquitous, but most commonly causes infection in patients with chronic underlying illnesses. the pathogenesis of human infection has not been definitively elucidated. bacteremia is the most common clinical manifestation of this infection, although cases of thrombophlebitis, mycotic aneurysm, endocarditis, and pericarditis have also be ...19902193344
isolation, characterization, and host-cell-binding properties of a cytotoxin from campylobacter jejuni.a 68,000-molecular-weight protein was isolated by polyacrylamide gel electrophoresis from the organism-free filtrate of a fully virulent clinical strain of campylobacter jejuni. the eluted protein was heat labile, was inactivated at either ph 3.0 or 9.0, was sensitive to trypsin, and was lethal for fertile chicken eggs. it also had toxic effects on chicken embryo fibroblast, chinese hamster ovary (cho), and intestinal 407 (int407) cells. a monoclonal antibody (cetpmab4) raised to this eluted tox ...19902199497
[occurrence and significance of different campylobacter types in domestic animals in hungary].experiences, including results of original experimental work on campylobacter fetus, c. jejuni and c. coli induced diseases of cattle, sheep, dogs, rabbits poultry and men in hungary are reviewed. out of 31 cases of abortion in cows 29 (93.5%) were causes by c. fetus subsp. venerealis and only one case each (3.2%) by c. fetus subsp. fetus and c. jejuni, respectively. out of the 29 strains of c. fetus subsp. venerealis, 26 belonged to serogroup 01 (a) and only 3 to serogroup 02 (b). campylobacter ...19902209454
surface array protein of campylobacter fetus. cloning and gene structure. 19902229082
campylobacter fetus infection of abdominal aortic aneurysm.a 61-year-old man with campylobacter fetus infection of an abdominal aortic aneurysm treated surgically is presented herein, the fifth survival case reported in the literature. fever and back pain preceded the enlargement of atherosclerotic abdominal aortic aneurysm. the patient tolerated satisfactorily total excision of the aneurysm followed by axillo-femoral prosthetic bypass. antibiotic therapy consisted of intravenous infusion of fosfomycin and gentamicin and oral administration of minocycli ...19902262502
fecal contamination of shanty town toddlers in households with non-corralled poultry, lima, peru.we used direct observer techniques to measure the frequency with which toddler-aged children were contaminated by poultry feces in homes in a peri-urban shanty town in lima, peru. the mean number of fowl was 5.4 (sd 3.1), with 10.0 (sd 10.7) poultry defecations per 12 hours. toddlers' hand contact with poultry feces occurred a mean of 2.9 (sd 3.0) times/12 hours. a mean of 3.9 (sd 4.6) feces-to-mouth episodes per household/12 hours occurred both by direct hand-to-mouth contamination and indirect ...19902297055
control of campylobacter fetus in artificially contaminated bovine semen by incubation with antibiotics before freezing.fresh, diluted semen containing 1.55 x 10(6) cfu/ml of campylobacter fetus subsp. venerealis was incubated with 500 iu of penicillin, 500 micrograms of streptomycin, 160 micrograms of lincomycin and 300 micrograms of spectinomycin per ml at 35 degrees c for 0, 1, 2, 5, 10, 20 or 40 minutes. the semen was cooled to 5 degrees c, packaged in 0.25 ml french straws and then frozen in liquid nitrogen for 2 weeks. immediately after thawing and removal of the antibiotics by centrifugation semen samples ...19902306605
horizontal transmission of campylobacter jejuni amongst broiler chicks: experimental studies.horizontal transmission of campylobacter jejuni was investigated in campylobacter-free broiler chicks. one hundred and twenty chicks housed individually, were provided with water containing 10(2)-10(9) c.f.u./ml c. jejuni. colonization was rapid [47 of 73 (64%) positive cloacal cultures within 3 days and 65 of 73 (89%) within 7 days], dependent on c. jejuni strain and inoculum size but independent of chick age. groups of 5-24 chicks in isolators were exposed to c. jejuni-contaminated water or co ...19902307180
pathogenesis of campylobacter fetus infections. role of surface array proteins in virulence in a mouse model.we developed a mouse model to compare the virulence of campylobacter fetus strains with (s-plus) and without (s-minus) surface array protein (s-protein) capsules. in adult ha/icr mice pretreated with ferric chloride, the ld50 for s-plus strain 84-32 was 43.3 times lower than its spontaneous s-minus mutant 84-54. seven strains of inbred mice were no more susceptible than the outbred strain. in contrast to the findings with salmonella typhimurium by others, 3 x 10(7) cfu of strain 84-32 caused 90% ...19902318963
campylobacter fetus infections in two patients with aids. 19902319151
factors influencing the duration of acute diarrheal disease in infancy.the majority of episodes of acute infectious diarrhea in infancy are of relatively short duration. prolongation of the diarrhea presents problems in management, deterioration of the infant's nutritional status, and an increased mortality. as part of a prospective study of the etiology of acute infectious diarrhea, some factors associated with the probability (more or less) of having self-limiting disease have been identified. the well-nourished infant over 6 months of age is more likely to have ...19902324877
[a case of right-sided infective endocarditis in a drug addict].a 49-year-old man was admitted to mitsui memorial hospital because of fever of unknown origin. since one year ago, he had often used intravenous narcotic drugs. ten months before his admission, he had his first experience of fever and a chill. four months later, he was admitted to a hospital under the diagnosis of pneumonia and he was treated successfully. after his discharge, he began to use drugs again, which resulted in the repetition of fever and chills during four months prior to his admiss ...19902330463
a domestic ferret model of immunity to campylobacter jejuni-induced enteric disease.oral or intravenous inoculation of previously unexposed juvenile and adult ferrets with campylobacter jejuni uniformly resulted in intestinal colonization lasting 2 to 12 days. disease varied from mild to moderate diarrhea, which resolved in 2 to 3 days. orally infected animals developed agglutinin titers of 8 to 256 within 3 weeks, while those infected intravenously developed titers of 256 to 2,048. ferrets which had recovered from campylobacteriosis all developed high titers of agglutinating a ...19902341180
bismuth subsalicylate in the prevention of colonization of infant mice with campylobacter jejuni.infant mice were used for the evaluation of the efficacy of bismuth subsalicylate (bss) in the prevention of the growth of campylobacter jejuni in the intestine. the mic90 of ten c. jejuni strains was 900 micrograms/ml. of three dosage regimens tested, continuous treatment before and after the bacterial challenge, mimicking the way bss is used in the prevention of traveller's diarrhoea, was the most effective. growth inhibition was dose dependent; the high dose of 2000 micrograms per day was mor ...19902347383
serogroups of campylobacter fetus and campylobacter jejuni isolated in cases of ovine abortion.of 38 aborted ovine fetuses from 23 sheep flocks 29 c. fetus subsp. fetus and 22 c. jejuni were isolated and examined biochemically and serologically for heat-stable antigens. serologic examinations were carried out by passive haemagglutination test. in case of c. fetus subsp. fetus strains alkaline antigen extraction was used. antisera to two serogroups of c. fetus and to penner serotype reference strains 1 to 60 were produced in rabbits. abortion was caused in 18 (78.3%) flocks by c. fetus sub ...19902363324
evidence of reinfection with multiple strains of campylobacter jejuni and campylobacter coli in macaca nemestrina housed under hyperendemic conditions.a prospective bacteriologic study of 18 infant pig-tailed macaques (macaca nemestrina) housed in a nursery facility in which campylobacter spp. are endemic was undertaken to determine the epidemiology of infection and reinfection. the isolates of campylobacter jejuni and c. coli cultured from 8 of the 18 infants were characterized by serotyping, dna hybridization, and polyacrylamide gel electrophoresis protein profiles. the chronology of infection was indicative of multiple reinfections with dif ...19902365455
infection of adult syrian hamsters with flagellar variants of campylobacter jejuni.two variants of campylobacter jejuni in1 differing in the expression of flagella, in1 (fla+ mot+, wild type) and in1-nm (fla- mot-), were tested for their ability to establish infection in adult hamsters. animals were challenged intracecally with 2 x 10(9) to 5 x 10(10) cfu and monitored for evidence of infection. none of the challenged animals developed illness. there was a significant difference, however, in the ability of in1 to infect hamsters (35 of 43) compared with that of in1-nm (1 of 42 ...19902365459
partial purification and characterization of the enterotoxin produced by campylobacter jejuni.campylobacter jejuni enterotoxin was partially purified from culture supernatant. the purified fraction after gel filtration indicated three bands at 68, 54, and 43 kilodaltons on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). this fraction enhanced the adenylate cyclase activity of hela cell membranes by 1.5-fold over that of the control. the study with anti-cholera toxin immunoglobulin g (igg) and ganglioside affinity column chromatographies revealed that the eluent from ...19902370099
polynucleotide sequence relationships among flagellin genes of campylobacter jejuni and campylobacter coli.dna probes that encode a complete flagellin gene and various internal regions of the campylobacter coli vc167 flagellin genes were hybridized to 30 strains of c. coli or c. jejuni from 20 different lior serogroups. the results indicated a high overall degree of homology among all of the strains examined. although the most variable regions occurred within the middle of the gene, significant dna homology was observed among many serogroups in this region of the molecule.19902370114
very long term diarrhoea due to campylobacter jejuni. 19902371198
purification, characterization, and localization of a protein antigen shared by thermophilic campylobacters.a protein antigen with an apparent molecular weight (mr) of 31,000 was isolated from 0.2 m glycine hydrochloride (ph 2.2) extracts of a typical human fecal isolate, campylobacter jejuni vc74. the protein was purified to homogeneity on a preparative scale by immunoaffinity chromatography followed by molecular sieving with a superose 12 column. isoelectric focusing under nondenaturing conditions indicated a pi of 9.3, and amino acid composition analysis showed that the protein was unusually rich i ...19902380360
use of an alkaline phosphatase-labeled synthetic oligonucleotide probe for detection of campylobacter jejuni and campylobacter coli.a commercially available synthetic nucleic acid probe (snap) conjugated to alkaline phosphatase was compared with standard culture techniques for detecting campylobacter species. the snap was able to detect either 5 ng of c. jejuni dna or 10(5) cfu of bacteria. the snap could also detect dna extracted from 10(5) cfu in mock-infected stool samples. the snap detected c. jejuni and c. coli but showed no reactivity with c. laridis, c. fetus subsp. fetus, c. fetus subsp. venerealis, c. fennelliae, "c ...19902380381
in vitro susceptibility of campylobacter jejuni to rokitamycin.the susceptibilities of 100 clinical isolates of campylobacter jejuni to rokitamycin, a new macrolide, and eight other oral antibiotics were tested by using a broth microdilution method. rokitamycin demonstrated antibacterial activity that was higher than those of fosfomycin and the tetracyclines and comparable to those of erythromycin and clindamycin, whereas it was slightly less active than the quinolones.19902386373
surface array proteins of campylobacter fetus block lectin-mediated binding to type a lipopolysaccharide.campylobacter fetus strains with type a lipopolysaccharide (lps) and a surface array protein layer (s+) have been found to be pathogenic in humans and animals. spontaneous laboratory mutants that lack surface array proteins (s-) are sensitive to the bactericidal activity of normal human serum. the ability of lectins to determine the presence of the s-layer and differentiate lps type was assessed. we screened 14 lectins and found 3 (wheat germ agglutinin, bandeiraea simplicifolia ii, and helix po ...19902387622
surface array protein of campylobacter fetus. cloning and gene structure.the high molecular mass (97-149 kda) surface array proteins (sap) of campylobacter fetus are critical to virulence. we created a bank of 160,000 random 1.0-6.5-kilobase (kb) chromosomal dna fragments of c. fetus strain 84-32 (23d) using lambda gt11. screening this bank in escherichia coli y1090 with antibody raised against purified sap permitted isolation and purification of a clone with a 4.0-kb insert. subcloning this insert in the e. coli vector, puc9, permitted expression of a protein of app ...19902387868
the development of a transport and enrichment medium for campylobacter fetus.a transport and enrichment medium was developed for campylobacter fetus. from inocula of between 10 and 35 organisms the medium was able to support the multiplication of 19 of 21 strains of c. fetus if the medium was incubated immediately after inoculation; when incubation was delayed for 3 days after inoculation, only seven of the 21 strains multiplied. from inocula of 100-350 organisms all 21 strains multiplied following immediate incubation, and 20 of 21 when incubation was delayed for 3 days ...19902397372
the application of a transport and enrichment medium to the diagnosis of campylobacter fetus infections in bulls.the use of a transport and enrichment medium (tem) in the diagnosis of campylobacter fetus infections in bulls is described. the medium significantly improved the diagnosis rate in samples which, because of the length of time between collection and receipt at the laboratory, were unsuitable for processing by direct culture. the tem was able to support the viability, and subsequent multiplication, of c. fetus in some samples for up to 7 days before the tem was incubated. the submission of paired ...19902397373
natural transformation in campylobacter species.growing cells of campylobacter coli and c. jejuni were naturally transformed by naked dna without the requirement for any special treatment. transformation frequencies for homologous chromosomal dna were approximately 10(-3) transformants per recipient cell in c. coli and 10(-4) in c. jejuni. maximum competence was found in the early log phase of growth. campylobacters preferentially took up their own dna in comparison with escherichia coli chromosomal dna, which was taken up very poorly. three ...19902404960
efficacy of a vaccine to prevent chlamydia- or campylobacter-induced abortions in a sheep flock, chlamydia psittaci, campylobacter fetus, ca jejuni, and salmonella dublin caused abortions. a vaccine that contained c psittaci type i from 2 sources: a cow with pneumonia and an aborted ovine fetus, ca fetus, ca jejuni, and 4 strains of k99 escherichia coli was given to 240 ewes before they were bred. all fetuses, placentas, and lambs, that died within 36 hours of birth were examined for infectious agents. of 55 abortions, 30 (55%) were caused by chlamydia or campylobacter spp ...19902407704
interaction of campylobacter jejuni and campylobacter coli with lectins and blood group antibodies.lectins and blood group antibodies were used to probe the surface structures of campylobacter jejuni and campylobacter coli. of the 29 strains tested, there were distinct reaction patterns. the lectin-reactive and blood group antibody-reactive sites on the bacterial surface were distinguishable from the heat-stable (lipopolysaccharide) antigenic determinants. the interactions were strain specific. the reactive sites were stable with respect to culture media and passage and may be useful as addit ...19852410445
basis for serological heterogeneity of thermostable antigens of campylobacter jejuni.lipopolysaccharides (lps) were extracted from eight strains of campylobacter jejuni and purified by enzyme treatment to remove traces of rna, dna, and protein. this material was used to sensitize sheep erythrocytes for the passive hemagglutination assay that is presently used to serotype c. jejuni. the results confirmed that the thermostable antigen typing scheme is based on lps (o) antigens. the lps after sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining was found to ...19852412962
monoclonal antibodies directed against the flagella of campylobacter jejuni: production, characterization and lack of effect on the colonization of infant mice.eight monoclonal antibodies have been derived from balb/c mice hyperimmunized with the purified flagella from campylobacter jejuni strain 81116. these monoclonal antibodies are directed against flagella as demonstrated by reaction in elisa against flagellate and aflagellate antigens, radio-immunoprecipitation and electro-immunoblotting techniques. some of the antibodies react with a 60k minor protein as well as the 62k flagella protein. this protein may be related to an antigen expressed on the ...19862422256
isolation and characterization of a common antigen in campylobacter jejuni and campylobacter coli.flagellin protein was isolated and purified from two serotype reference strains of campylobacter jejuni, pen 1 and pen 3. each preparation was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to consist of a diffuse band with a molecular mass of approximately 62 kilodaltons. antisera were prepared against flagellin from pen 1, and specific antibody was isolated by affinity chromatography with flagellin protein covalently bound to cyanogen bromide-activated sepharose. the high-a ...19862424932
location of epitopes on campylobacter jejuni flagella.flagella were isolated from strains of campylobacter jejuni belonging to different heat-labile serogroups and from a strain of campylobacter fetus, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the flagellin molecular weights (mr) were approximately 62,000. the flagellins were cleaved by hydrolysis with cyanogen bromide, and sodium dodecyl sulfate-urea peptide gel electrophoresis showed that the c. jejuni flagellins were structurally similar, and differed from c. fetu ...19862430943
experimental production of proliferative ileitis in syrian hamsters (mesocricetus auratus) by using an ileal homogenate free of campylobacter jejuni.the role of campylobacter jejuni in the pathogenesis of proliferative ileitis of syrian hamsters (mesocricetus auratus) has been uncertain. c. jejuni has been implicated as the etiologic agent on the basis of the campylobacter-type morphology of the intracellular organism and the repeated microbiologic isolation of c. jejuni from hamsters with proliferative ileitis. the inability to reproduce the disease with pure culture inocula, coupled with immunohistochemical studies, however, has suggested ...19892462537
flagellin expression in campylobacter jejuni is regulated at the transcriptional level.campylobacter jejuni 81116 is able to switch flagellum formation on and off. to study the expression of flagellin, the main component of flagella, an expression library of c. jejuni dna was constructed in lambda gt11. screening of this library with a flagellin-specific antiserum resulted in a clone producing a beta-galactosidase-flagellin fusion protein; it contained a dna insert of 850 base pairs and coded for about 15 kilodaltons of the flagellin, corresponding to 410 base pairs of the flagell ...19892466792
prospective study of campylobacter jejuni infection in chilean infants evaluated by culture and serology.a prospective study of campylobacter jejuni infection was performed during a 6-month period in a cohort of 198 chilean infants. surveillance was based on biweekly home visits by a team of trained nurses. c. jejuni colonization was studied by culturing stool samples from diarrheal episodes and paired asymptomatic controls and by performing an enzyme-linked immunosorbent assay developed to measure c. jejuni outer membrane protein polyvalent antibodies in a representative group of infants. c. jejun ...19892473090
[sepsis caused by campylobacter fetus spp. fetus]. 19892491527
pancreatitis associated with campylobacter jejuni infection: diagnosis by ultrasonography. 19892499366
[the most frequent infectious causes of abortion in sheep in north bavaria with special reference to chlamydia and salmonella infections].between 1980 and 1987 1153 ovine fetuses and placentas were examined after abortion. in 68.5% of the cases a cause of abortion could be diagnosed as follows: 43.5% chlamydia psittaci var. ovis, 10.7% salmonella abortus ovis, 3.7% coxiella burnetii, 3.3% listeria monocytogenes and 0.4% campylobacter fetus intestinalis. hemolyzing escherichia coli and streptococci, yersinia pseudotuberculosis, salmonella typhimurium, salmonella enteritidis, corynebacterium pyogenes and other facultative pathogens ...19892503908
rapid method for the detection of dnase of campylobacters.a rapid agar diffusion method for the detection of dnase production of campylobacter jejuni, c. coli, and c. pylori was developed. a strong pink zone indicating dna hydrolysis was seen around the wells after 20 to 24 h of aerobic incubation at 37 degrees c. pretreatment of cells with polymyxin b, which releases the cell-associated dnase, both shortened the time needed to read positive results to 8 h and increased the zone size.19892506224
abdominal aortic aneurysm infected with campylobacter fetus subspecies fetus.we report a survivor of campylobacter fetus septicemia from an infected abdominal aortic aneurysm who was successfully treated with an anatomic graft reconstruction and antibiotics. according to a survey of the english-language medical literature this was the fourth such patient successfully treated. c. fetus sepsis associated with an abdominal aortic aneurysm was first reported in 1971. the first patient to survive reconstruction of an aortic tube graft aneurysm infected with c. fetus was repor ...19892527318
antigenic and restriction enzyme analysis of isolates of campylobacter fetus subsp venerealis recovered from persistently infected cattle.thirty-two isolates of campylobacter fetus subsp venerealis were obtained from 1 bull and 4 heifers with experimentally induced infection. when whole-cell antigens of isolates were cross titrated with antisera to the infecting strain, isolates from 3 heifers had limited antigenic variation, whereas whole-cell antigens of isolates from 2 cattle (the bull and a heifer) differed serologically from those of the infecting strain. changes were detected specifically in 6 heat-labile antigens. of the 6 ...19892548417
adhesion to and invasion of hep-2 cells by campylobacter spp.twenty-one isolates were tested for their ability to adhere to and invade hep-2 cells in vitro. of the 21 organisms tested, 2 did not invade the hep-2 cells, and 1 of these did not adhere to the epithelial cells. campylobacter jejuni clinical isolates were more invasive than the nonclinical strains that were tested. when hep-2 cells were treated with cytochalasin b, the invasiveness of c. jejuni was reduced, indicating active participation of the host cell in the uptake of these organisms. the n ...19892550368
antigenic analysis of campylobacter flagellar protein and other proteins.outer membrane proteins of campylobacter jejuni and other campylobacter species were analyzed for their antigenic potentials by immunoblotting. polypeptides were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred electrophoretically, and reacted with rabbit antisera to c. jejuni. each campylobacter species analyzed demonstrated a unique outer membrane protein antigenic profile; interspecies antigen sharing was observed to be compatible with the degree of dna relat ...19852578478
western blot analysis of the human antibody response to campylobacter jejuni cellular antigens during gastrointestinal infection.western blot analysis was used to identify antigenic components of campylobacter jejuni whole cells and outer membranes that elicit antibody responses in patients with campylobacter enteritis. acute- and convalescent-phase sera from eight patients were analyzed for antibody activity against their homologous infecting strains and heterologous clinical isolates. whole-cell and sarkosyl-insoluble membrane components were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and tra ...19852578479
antigenic heterogeneity of lipopolysaccharides from campylobacter jejuni and campylobacter fetus.the lipopolysaccharide (lps) structure of campylobacter spp. can be visualized with polyacrylamide gel electrophoresis by examining proteinase k-treated whole cell lysates. polyacrylamide gel electrophoresis lps profiles of c. jejuni strains are rough type with low concentrations of low-molecular-weight polysaccharide side chains, serum-resistant c. fetus strains have smooth-type lps, and serum-sensitive c. fetus strains have rough-type lps. we electroblotted the proteinase k-treated whole cell ...19852580793
human antibody response to campylobacter jejuni flagellin protein and a synthetic n-terminal flagellin peptide.we measured isotype-specific human antibodies directed against campylobacter jejuni native flagellin and a synthetic peptide derived from the n-terminal amino acid sequence of the protein by using a microdilution enzyme-linked immunosorbent assay (elisa). serum samples from patients with gastrointestinal infection caused by c. jejuni (n = 20) and control samples (number from normal subjects = 20; number from patients with diarrhea other than campylobacter = 20) were tested in this assay. serum s ...19892584372
bacterial infertility in camels (camelus dromedarius): isolation of campylobacter fetus.this study constitutes the first reported isolation of c. fetus subsp. venerealis from cervical swabs in breeding camels. 9 of 30 camels which were barren for a long period were found to be positive for c. fetus. the methods of isolation are described. additionally, cervical flora of 30 camels were examined. 9 different bacterial species were isolated of which staphylococcus aureus was most frequently found. all samples were negative for trichomonas sp. and streptococcus zooepidemicus.19892598805
response of the chick embryo to live and heat-killed campylobacter jejuni injected into the yolk sac.graded doses of live and heat-killed cells of campylobacter jejuni were injected into the yolk-sac of 5-day-old chick embryos, and the 50% lethal dose (ld50) was determined 7 days later. a strain dependent virulence was seen. in the diluted series of cultures the ld50 values for live campylobacter ranged from 10(6) c.f.u. beyond the last dilution showing growth, that is to less than one organism per embryo. when the 22 strains were tested as heat-killed cells, the chick embryo ld50 values retain ...19892606163
[infectious diarrhea diseases].gastroenteritis may be due to many agents, infectious or non infections. most cases of infections diarrhea are selflimited--e.g. food born intoxications with 1 to 2 days duration or infections with enteropathogenic e. coli, salmonella spp., vibrio parahaemolyticus or rota virus. even the majority of shigellosis, salmonella-gastroenteritis and infections with campylobacter fetus cease without treatment within 4 days. fever and diarrhea persisting for more than 4 days justify the use of antibiotic ...19892662618
[neurologic involvement in campylobacter infections. 5 cases].campylobacter are a newly recognized class of human infectious agents. campylobacter fetus subspecies fetus is a cause of sepsis in immunocompromized hosts. secondary neurological determinations, meningitis and meningoencephalitis appear to be rare. we report 2 cases, and 8 previously reported cases are reviewed. campylobacter jejuni appears to be a common bacterial pathogen causing a syndrome of enteritis. since 1982 it has been associated with guillain-barré syndrome (gbs). in one serological ...19892664975
campylobacter fetus subspecies fetus infection of an abdominal aneurysm.we report a case of aneurysm of the abdominal aorta with spontaneous rupture in a 64-year-old man with emphysema and chronic alcoholism. campylobacter fetus subspecies fetus was repeatedly recovered from the blood and from specimens of the excised aneurysm. the patient died in spite of early diagnosis, intravenous erythromycin and surgical intervention. the bacteriology and epidemiology of campylobacteriosis in man is briefly discussed and eight other published cases of aneurysm, infected with t ...19892678860
applications of a new method for devising bacteriological serotyping schemes.greatly simplified serotyping schemes were devised for two organisms, escherichia coli and campylobacter jejuni. the mathematical approach developed to improve the e. coli serotyping routine at the national health institute in new zealand was used on three previously published data sets. it had been considered possible that the mathematical method used in the first study was specific to that data set and therefore not applicable elsewhere. this paper verifies that the mathematical method works o ...19892685036
campylobacter fetus subsp. jejuni in poultry reared under different management systems in nigeria.cloacal swabs from 487 live birds in 36 flocks and 70 poultry carcasses were cultured for campylobacter fetus subsp. jejuni. it was isolated from 12.3% of the birds in 19 flocks. chickens, turkeys, and guinea fowl differed from one another in isolation rates of the organism. management system affected its occurrence, and only 7.1% of eviscerated carcasses yielded it. it was concluded that bird species, management system, and immersing slaughtered poultry in boiling water before dressing affect r ...19892695052
[a case report of campylobacter fetus subspecies fetus bacteremia].an unusual case of campylobacter fetus subspecies fetus bacteremia was presented. a twenty four year old male was admitted to our hospital due to abdominal pain, general malaise, diarrhea, high fever, and hemoptysis. he was alcoholic and fond of eating raw liver. he had a history of partial gastrectomy and disturbance of pancreatic function. he showed pulmonary empyema, pleuritis, thrombophlebitis of lower legs, jaundice, hepatomegaly, diarrhea, pneumothorax, and low t3 low t4 syndrome. c. fetus ...19892695582
serotyping studies of campylobacter from naturally colonized chickens.campylobacter jejuni/coli strains from 164 chickens were serotyped by the methods previously described by penner et al. and lior et al. the chickens were sampled during breeding from hatching to the age of 42 weeks. the birds were housed, in two separate groups, under different environmental conditions, (for comparison of the effect of hygienic precautions on the transmission of the bacteria during breeding). in the group where the hygienic conditions could be controlled to a greater extent, the ...19892703018
comparison of different vaccines and induced immune response against campylobacter jejuni colonization in the infant mouse.the degree of protection conferred by vaccinated dams on infant mice against colonization by campylobacter jejuni depended on the bacterial strain, preparation, and route of administration of the vaccine. in some instances of homologous protection, serum bactericidal titres correlated well with protection. however, boiled c. jejuni vaccine, which was non-protective, also elicited a strong bactericidal antibody response. conversely, bactericidal activity could not be demonstrated against strains ...19892703020
population genetics of human and animal enteric campylobacter strains.a total of 125 strains of campylobacter jejuni and campylobacter coli from human and animal hosts were analyzed by multilocus enzyme electrophoresis. ten genetic loci were characterized by the electrophoretic mobilities of their products, and all were found to be polymorphic. the nine loci considered for genetic analysis had an average allele number of 6.9 and a mean genetic diversity of 0.634. the strains were grouped into 64 electrophoretic types (ets) forming two principal clusters; the first ...19892707852
experimental campylobacter jejuni infection in macaca nemestrina.experimental infection of four specific-pathogen-free macaca nemestrina monkeys (aged 3.5 and 4.5 months) with campylobacter jejuni 81-176 caused acute diarrheal illness, characterized by fluid diarrhea, bloody stools, and fecal leukocytes, which lasted for approximately 7 to 11 days. histologic examination of intestinal biopsies showed acute colitis characterized by infiltration of the mucosa with neutrophils and lymphocytes, and cryptitis. there were no histologic changes in the small intestin ...19892707853
campylobacter fetus as a pulmonary pathogen in man. case report of post-pneumonectomy empyema. 19892710441
cellular events and intracellular survival of campylobacter jejuni during infection of hep-2 cells.invasion and intracellular survival of campylobacter jejuni in hep-2 cells were analyzed by transmission electron microscopy and by viable counts after killing of extracellular bacteria by gentamicin. during the first 30 min after challenge, no bacteria were seen in association with the host cell. after 1 h, campylobacters apparently attached to the cell membrane, with areas of close appositions. in these areas, an intracellular network of actin-like filaments was seen beneath the plasma membran ...19892731988
colonization of mice by campylobacter jejuni.both streptomycin-treated and untreated swiss white mice were irregularly colonized when challenged orogastrically with between 1 and 10(11) viable organisms of either of two strains of campylobacter jejuni. the organisms were occasionally recovered from portions of the intestinal tracts of these animals in numbers ranging from 10(1) to 10(3)/g when the challenge doses were 10(10) or more. when germfree mice were challenged with 10(8) organisms of either strain, the entire intestinal tracts of a ...19892744846
immune response to campylobacter jejuni and campylobacter coli in a cohort of children from birth to 2 years of age.a cohort of 111 children from bangui, central african republic, was surveyed for enteric campylobacter infections from birth to the age of 2 years; stools were examined biweekly in these children until 6 months of age and at least four times per year thereafter until 2 years of age and after each diarrheal episode. blood samples were obtained at birth and at 3, 6, 9, 12, 18, and 24 months of age. antibodies against glycine-extracted membrane antigens, purified flagella, and cholera toxin (ct) we ...19892744860
hexagonal surface layer of campylobacter fetus isolated from humans.electron microscopic studies of the surface structure of campylobacter fetus by the freeze-etching method showed two different types of s layer. one was in a hexagonal array, and the other was in a tetragonal array. a high-passage-number strain lost its s layer during cultivation on culture media but regained it after a single animal passage.19892744862
identification of campylobacter jejuni and c. coli by gel electrophoresis of the outer membrane proteins.analysis of the electrophoretic profiles of the outer membrane proteins could be used to differentiate campylobacter jejuni (16 strains) from campylobacter coli (10 strains). this observation was confirmed by the study of dna homology obtained by a quantitative filter hybridization method. the hippurate hydrolysis test gave a poor correlation with the results of differentiation obtained by dna homology studies and outer membrane protein profile.19892745680
cellular fatty acid composition of campylobacter pylori from primates and ferrets compared with those of other campylobacters.the cellular fatty acid profiles of newly described campylobacters were determined on a polar, capillary column. six isolates of the gastric spiral organism, campylobacter pylori subsp. mustelae, from ferrets from australia, england, and the united states were all found to have a similar fatty acid profile which was different from that of c. pylori from humans; c. pylori subsp. mustelae did not have 3-hydroxyoctadecanoic acid (3-oh c18:0) and had much less tetradecanoic acid (c14:0) and much mor ...19892745703
cases of infective endocarditis caused by campylobacter 18-year-old man with infective endocarditis caused by campylobacter fetus is reported. this bacterial species has long been of interest in veterinary medicine, and recently it has been reported to be one cause of infantile diarrhea. infective endocarditis arising from campylobacter fetus is rare. this is the fourth case reported in japan. the organism was resistant to several antibiotics, and large vegetations were found to involve the aortic valve. emergency excision of the infected aortic v ...19892748151
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