PMID(sorted descending)
pathogenesis of campylobacter fetus infections. role of surface array proteins in virulence in a mouse model.we developed a mouse model to compare the virulence of campylobacter fetus strains with (s-plus) and without (s-minus) surface array protein (s-protein) capsules. in adult ha/icr mice pretreated with ferric chloride, the ld50 for s-plus strain 84-32 was 43.3 times lower than its spontaneous s-minus mutant 84-54. seven strains of inbred mice were no more susceptible than the outbred strain. in contrast to the findings with salmonella typhimurium by others, 3 x 10(7) cfu of strain 84-32 caused 90% ...19902318963
horizontal transmission of campylobacter jejuni amongst broiler chicks: experimental studies.horizontal transmission of campylobacter jejuni was investigated in campylobacter-free broiler chicks. one hundred and twenty chicks housed individually, were provided with water containing 10(2)-10(9) c.f.u./ml c. jejuni. colonization was rapid [47 of 73 (64%) positive cloacal cultures within 3 days and 65 of 73 (89%) within 7 days], dependent on c. jejuni strain and inoculum size but independent of chick age. groups of 5-24 chicks in isolators were exposed to c. jejuni-contaminated water or co ...19902307180
control of campylobacter fetus in artificially contaminated bovine semen by incubation with antibiotics before freezing.fresh, diluted semen containing 1.55 x 10(6) cfu/ml of campylobacter fetus subsp. venerealis was incubated with 500 iu of penicillin, 500 micrograms of streptomycin, 160 micrograms of lincomycin and 300 micrograms of spectinomycin per ml at 35 degrees c for 0, 1, 2, 5, 10, 20 or 40 minutes. the semen was cooled to 5 degrees c, packaged in 0.25 ml french straws and then frozen in liquid nitrogen for 2 weeks. immediately after thawing and removal of the antibiotics by centrifugation semen samples ...19902306605
fecal contamination of shanty town toddlers in households with non-corralled poultry, lima, peru.we used direct observer techniques to measure the frequency with which toddler-aged children were contaminated by poultry feces in homes in a peri-urban shanty town in lima, peru. the mean number of fowl was 5.4 (sd 3.1), with 10.0 (sd 10.7) poultry defecations per 12 hours. toddlers' hand contact with poultry feces occurred a mean of 2.9 (sd 3.0) times/12 hours. a mean of 3.9 (sd 4.6) feces-to-mouth episodes per household/12 hours occurred both by direct hand-to-mouth contamination and indirect ...19902297055
campylobacter fetus infection of abdominal aortic aneurysm.a 61-year-old man with campylobacter fetus infection of an abdominal aortic aneurysm treated surgically is presented herein, the fifth survival case reported in the literature. fever and back pain preceded the enlargement of atherosclerotic abdominal aortic aneurysm. the patient tolerated satisfactorily total excision of the aneurysm followed by axillo-femoral prosthetic bypass. antibiotic therapy consisted of intravenous infusion of fosfomycin and gentamicin and oral administration of minocycli ...19902262502
surface array protein of campylobacter fetus. cloning and gene structure. 19902229082
[occurrence and significance of different campylobacter types in domestic animals in hungary].experiences, including results of original experimental work on campylobacter fetus, c. jejuni and c. coli induced diseases of cattle, sheep, dogs, rabbits poultry and men in hungary are reviewed. out of 31 cases of abortion in cows 29 (93.5%) were causes by c. fetus subsp. venerealis and only one case each (3.2%) by c. fetus subsp. fetus and c. jejuni, respectively. out of the 29 strains of c. fetus subsp. venerealis, 26 belonged to serogroup 01 (a) and only 3 to serogroup 02 (b). campylobacter ...19902209454
isolation, characterization, and host-cell-binding properties of a cytotoxin from campylobacter jejuni.a 68,000-molecular-weight protein was isolated by polyacrylamide gel electrophoresis from the organism-free filtrate of a fully virulent clinical strain of campylobacter jejuni. the eluted protein was heat labile, was inactivated at either ph 3.0 or 9.0, was sensitive to trypsin, and was lethal for fertile chicken eggs. it also had toxic effects on chicken embryo fibroblast, chinese hamster ovary (cho), and intestinal 407 (int407) cells. a monoclonal antibody (cetpmab4) raised to this eluted tox ...19902199497
cardiovascular and bacteremic manifestations of campylobacter fetus infection: case report and review.a case of bacteremia due to campylobacter fetus subspecies fetus with concomitant pleuropericarditis in a previously healthy patient is presented. the organism is ubiquitous, but most commonly causes infection in patients with chronic underlying illnesses. the pathogenesis of human infection has not been definitively elucidated. bacteremia is the most common clinical manifestation of this infection, although cases of thrombophlebitis, mycotic aneurysm, endocarditis, and pericarditis have also be ...19902193344
use of pulsed-field agarose gel electrophoresis to size genomes of campylobacter species and to construct a sali map of campylobacter jejuni determine the physical length of the chromosome of campylobacter jejuni, the genome was subjected to digestion by a series of restriction endonucleases to produce a small number of large restriction fragments. these fragments were then separated by pulsed-field gel electrophoresis with the contour-clamped homogeneous electric field system. the dna of c. jejuni, with its low g+c content, was found to have no restriction sites for enzymes noti and sfii, which cut a high-g+c regions. most of the ...19902168376
identification of campylobacter jejuni surface proteins that bind to eucaryotic cells in understand the role of campylobacter jejuni surface proteins in the interaction of c. jejuni with cultured mammalian cell lines in vitro, we developed a ligand-binding assay. this procedure allowed us to antigenically identify c. jejuni outer membrane proteins (omps) that attach to intact host cell membranes. omps isolated from an invasive strain and a less invasive strain were antigenically indistinguishable. however, we found that proteins with molecular masses of 28 and 32 kilodaltons (kda ...19902160431
hydrophobic characterisation of helicobacter (campylobacter) pylori.cell-surface hydrophobicity of helicobacter (formerly campylobacter) pylori was tested by aqueous two-phase partitioning and hydrophobic interaction chromatography. the hydrophobicity of h. pylori greatly exceeded that of campylobacter fetus subsp. fetus, c. jejuni and bacillus subtilis. a partition coefficient (pc) of hydrophobicity in the two-phase system was determined for h. pylori. pc was dependent on ph and the pc value was increased by greater than 20-fold at ph 2.5. lithium cations incre ...19902113100
microbiological quality and safety of zoo food.two types of commercial products for feeding zoo animals (a frozen meat product, referred to as zoo food, and a dry product, referred to as dry food) were microbiologically examined for spoilage organisms (aerobic, psychrotrophic, coliform, escherichia coli, mold, and yeasts) and pathogens (salmonella spp., listeria monocytogenes, and campylobacter jejuni). levels of microorganisms in frozen ground zoo food were compared with those in frozen ground beef and frozen ground turkey meat. the level o ...19902111115
[pericarditis caused by campylobacter fetus fetus]. 19902099869
adherence of helicobacter pylori cells and their surface components to hela cell membranes.four helicobacter pylori strains were used to develop in vitro methods to assess adherence to hela cells. using direct detection by microscopy, adhesion scores increased with the initial bacteria-to-cell ratio. the urease method assessed h. pylori bound to hela cells by their urease activity. the percentage of the original inoculum adhering to hela cells remained constant for initial ratios from 10(2) to 10(5) bacteria per cell. an elisa using anti-h. pylori serum assessed whole bacteria or comp ...19902097496
[bacteremia caused by campylobacter fetus ssp fetus in an hiv-positive patient with liver cirrhosis]. 19902090247
[bacteremia caused by campylobacter fetus ssp fetus in a patient with liver cirrhosis]. 19902090246
campylobacter fetus subspecies fetus meningitis with chronic alcoholism and diabetes mellitus.a case of campylobacter fetus subspecies fetus meningitis is reported. the patient had underlying diseases, namely chronic alcoholism and diabetes mellitus. the infection did not respond to piperacillin and cefotaxime, but did respond to ampicillin and moxalactam. the patient was discharged on the 33rd hospital day showing no neurological deficit complications, and has remained free of recurrent disease for one month after the discontinuation of therapy.19902089181
[endocarditis caused by campylobacter fetus]. 19912051801
correlation between molecular size of the surface array protein and morphology and antigenicity of the campylobacter fetus s layer.the correlation between the molecular size of the surface layer protein (s protein) and both structure and antigenicity of the campylobacter fetus surface layer (s layer) was investigated in several clinical strains and their spontaneous variants which produce s proteins of molecular weights (mw) different from those of the parents. only three molecular sizes of the s proteins were observed (98, 127, and 149 kda) in the parental and variant strains. immunologically, the 98-kda protein and the 14 ...19912037362
[campylobacter fetus ssp fetus septicemia associated with a colonic abscess].campylobacter is considered to be an opportunistic agent. the authors relate an unusual case with campylobacter fetus ssp fetus (cf) septicemia and colic abscess. human campylobacteriosis is presumed to be a food-born disease related to contaminated animal products such as milk or meat. in some cases cf may be transmitted by drinking water or by fecal soiling via the hands. conventional treatment uses macrolides with decrease the duration of diarrhea and reduce the fecal excretion of cf. macroli ...19912029182
freeze-substitution of gram-negative eubacteria: general cell morphology and envelope profiles.freeze-substitution was performed on strains of escherichia coli, pasteurella multocida, campylobacter fetus, vibrio cholerae, pseudomonas aeruginosa, pseudomonas putida, aeromonas salmonicida, proteus mirabilis, haemophilus pleuropneumoniae, caulobacter crescentus, and leptothrix discophora with a substitution medium composed of 2% osmium tetroxide and 2% uranyl acetate in anhydrous acetone. a thick periplasmic gel ranging from 10.6 to 14.3 nm in width was displayed in e. coli k-12, k30, and hi ...19911999383
multivariate analyses of cellular fatty acids in bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp.the genera bacteroides, wolinella, and campylobacter contain several similar species that require taxonomic revision. fatty acid profiles of whole bacterial cells have proven useful for taxonomy. in this study, cellular fatty acids from bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp. were identified and quantitated by gas chromatography and gas chromatography-mass spectrometry, and the data were subjected to principal component analyses. bacteroides fragilis, the type s ...19911993755
diagnosis by elisa of bovine abortion due to campylobacter enzyme-linked immunosorbent assay (elisa) was developed to detect antigen-specific secretory iga antibody in bovine vaginal mucus after abortion due to campylobacter fetus subsp venerealis. abortions were diagnosed by isolating the organism from 8 foetuses and/or foetal membranes and by histopathology. vaginal mucus was collected from 7 cows shortly after abortion. all showed a high level of iga antibody in their vaginal mucus when they were compared with an uninfected control group. the new ...19911953551
[bacteremia from campylobacter fetus. increasing interest and incidence].three bacteremias of campylobacter fetus were described. one was a male patient and two were females. underlying illnesses were present in all of them (hodgkin disease, aids and hepatic cirrhosis respectively). they were all admitted because of fever and no other symptoms of infectious focus were present. physical findings were not relevant. the blood cultures became positive in days 6, 7 and 9 respectively. antibiotic treatments were not standardised, so no conclusions can be drawn. the evoluti ...19911943296
campylobacter spondylodiscitis and deficiency of cellular immunity.we describe a case of spondylodiscitis caused by campylobacter fetus in a previously healthy patient. this is the first report of this agent causing discovertebral infection, which generated cellular immune deficiency, reversible with the cure.19911795335
comparison of surface antigens of some campylobacter fetus subsp. fetus strains of ovine origin by polyacrylamide gel electrophoresis and immunoblotting.sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and immunoblotting were used to identify and to compare the surface antigens of eight c. fetus subsp. fetus strains. seven strains (one of serogroup a and six of serogroup b) were isolated from aborted ovine fetuses, while one strain (serogroup a) originated from an aborted calf fetus. saline extracts at 56 degrees c and 100 degrees c were used as antigens. antisera were produced in rabbits. in saline extracts (56 degrees c) of ...19911776377
prevalence of bovine campylobacteriosis in indigenous cattle of three states in nigeria.a survey of bovine campylobacteriosis in breeding bulls and cows was carried out in the states of kaduna, kano and borno. six hundred and eighty nine cattle composed of 585 and 104 breeding bulls and cows respectively were sampled. campylobacter fetus subsp. venerealis was isolated from 12 bulls while campylobacter fetus subsp. fetus was isolated from three of them. campylobacter fetus subsp. fetus was isolated from four cows while campylobacter fetus subsp. veneralis was isolated from one cow. ...19911763437
campylobacter fetus subspecies fetus infection.during a six-year period five patients with campylobacter fetus subspecies fetus infections were seen at the mayo clinic in rochester, minnesota. bacteremia was observed in two patients, one presenting with aortic valve endocarditis and the other with abdominal atherosclerotic aortic aneurysm. c. fetus subsp. fetus was isolated from tibial tissue of a patient with osteomyelitis. diarrhea was the main complaint of two further patients, and was also mentioned by the patient with the aortic aneurys ...19911762387
characterization of an immunoreactive species-specific 19-kilodalton outer membrane protein from helicobacter pylori by using a monoclonal antibody.immunoblotting experiments on hyperimmune rabbit serum and sera from patients with helicobacter pylori gastritis showed a consistent antibody response to a 19-kda outer membrane protein antigen. a monoclonal antibody, designated hp 40, which reacted by western immunoblotting with this protein was produced. it was shared by all h. pylori strains tested (d 273, nctc 11637, and 24 wild strains) but not by the thermophilic campylobacter species, campylobacter fetus, helicobacter mustellae, or helico ...19911761682
reattachment of surface array proteins to campylobacter fetus cells.campylobacter fetus strains may be of serotype a or b, a property associated with lipopolysaccharide (lps) structure. wild-type c. fetus strains contain surface array proteins (s-layer proteins) that may be extracted in water and that are critical for virulence. to explore the relationship of s-layer proteins to other surface components, we reattached s-layer proteins onto s- template cells generated by spontaneous mutation or by serial extractions of s+ cells with water. reattachment occurred i ...19921735716
oligodeoxynucleotide probes for campylobacter fetus and campylobacter hyointestinalis based on 16s rrna sequences.deoxyoligonucleotide probes were constructed for the identification of campylobacter fetus and campylobacter hyointestinalis based on 16s rrna sequence data. probes were targeted to hypervariable regions of 16s rrna. specificity of oligonucleotide probes was tested in a colony blot assay with type strains of 15 campylobacter and arcobacter species as well as in a slot blot format using genomic dna extracted from field strains of c. fetus and c. hyointestinalis. two oligonucleotides were construc ...19911723076
revision of campylobacter, helicobacter, and wolinella taxonomy: emendation of generic descriptions and proposal of arcobacter gen. nov.hybridization experiments were carried out between dnas from more than 70 strains of campylobacter spp. and related taxa and either 3h-labeled 23s rrnas from reference strains belonging to campylobacter fetus, campylobacter concisus, campylobacter sputorum, campylobacter coli, and campylobacter nitrofigilis, an unnamed campylobacter sp. strain, and a wolinella succinogenes strain or 3h- or 14c-labeled 23s rrnas from 13 gram-negative reference strains. an immunotyping analysis of 130 antigens ver ...19911704793
antigenic differences among campylobacter fetus s-layer proteins.sodium dodecyl sulfate-polyacrylamide gel electrophoresis of s-layer proteins extracted from campylobacter fetus strains by using acid glycine buffer showed that the predominant s-layer proteins of different strains had subunit molecular weights in the range of 90,000 to 140,000. electron microscopy revealed oblique s-layer lattices with a spacing of approximately 5.6 nm (gamma = 75 degrees) on wild-type strains vc1, vc119, vc202, and vc203. three variants of c. fetus vc119 producing a predomina ...19901697574
[campylobacter fetus spondylodiscitis].the case of a sixty-two year old man with campylobacter fetus spondylodiscitis is reported. despite of adequate antibiotherapy, the outcome was bad and death occurred. three other cases of campylobacter fetus spondylodiscitis are reported in the literature. tomodensitometry disclosed a paravertebral abscess with gas, that make our observation original.19921579850
neonatal campylobacter fetus meningitis: a report of an unusual case.campylobacter fetus is a rare cause of meningitis in the pediatric age group and, in particular, among neonates. the clinical presentation of campylobacter meningitis in high-risk neonates is not well-described. a review of campylobacter meningitis by lee et al in 1985 reported nine cases occurring in neonates, of which only one case was caused by c. fetus. in the ensuing six years only three more confirmed cases of neonatal meningitis caused by c. fetus have been reported. we here report anothe ...19921563204
characterization of the campylobacter fetus sapa promoter: evidence that the sapa promoter is deleted in spontaneous mutant strains.wild-type campylobacter fetus cells possess s-layer proteins (s+ phenotype), whereas after laboratory passage, spontaneous stable mutants that do not express these proteins (s- phenotype) arise. to determine the molecular mechanisms by which c. fetus changes to the s- phenotype, we studied wild-type strain 23d, from which the sapa gene encoding the 97-kda s-layer protein has been cloned, and strain 23b, a spontaneous s- mutant. we compared these strains with another pair of strains, lp (s+) and ...19921522068
[acute aortic insufficiency following endocarditis due to infection with campylobacter fetus subspecies fetus].a 50-year-old male alcoholic addict, examined because of diarrhoea with fever was found to have campylobacter jejuni in blood and stool cultures. after administration of broad-spectrum penicillin all acute symptoms disappeared but he lost 8 kg within 3 months and his general state health gradually deteriorated. after 3 months he suddenly developed leg oedema, dyspnoea and bouts of fever up to 38.8 degrees c. a loud cardiac murmur was now heard. echocardiography demonstrated vegetations on the re ...19921516529
[human campylobacteriosis caused by campylobacter fetus]. 19921507827
pyogenic arthritis of the hip due to campylobacter fetus--a case report.septic arthritis of the hip caused by campylobacter fetus subsp. fetusis very rare. the authoris isolated c. fetus subsp. fetus from a specimen of the left hip. the patient was a 53-year old man with a history of heavy drinking, diabetes, and chronic hepatitis, and had been suffering from avascular necrosis of both femoral heads. it was considered that the organism invaded already damaged tissue of the joint. the patient was treated with intravenous antibiotics and later received successful tota ...19921502834
clinical evaluation of the efficacy of inoculating cattle with a vaccine containing tritrichomonas test the efficacy of a polyvalent tritrichomonas foetus vaccine, 130 nulliparous heifers were randomly assigned to either receive the test t foetus vaccine or to serve as nonvaccinated controls. the polyvalent test vaccine consisted of a campylobacter fetus/leptospira canicola-grippotyphosa-hardjo-icterohaemorrhagiae-pamona bacterine containing 5 x 10(7) killed t foetus/dose. the polyvalent control vaccine consisted of the aforementioned formulation without t foetus. heifers were administered ...19921466496
structural studies of peptidoglycans in campylobacter species.peptidoglycans (pg) from campylobacter coli, campylobacter jejuni, and campylobacter fetus were composed of muramic acid, glucosamine, alanine, glutamic acid, and diaminopimelic acid in a molar ratio of 1.1:1:1.7:1.1:09. thirty percent of the amino groups of diaminopimelic acid were involved in cross-linkages between peptides. during cultivation, c. coli and c. jejuni changed from a spiral to a coccoid form. in c. coli, we could isolate pg only from the spiral forms in yields of 0.8-1.2% by dry ...19921461153
anti-enterobacteria antibodies in psoriatic arthritis.the occurrence of certain antibacterial antibodies was studied in the sera of 22 healthy donors (hd) and 66 patients with different diseases. the cases investigated included 22 rheumatoid arthritis (ra), 22 non-arthritic-psoriasis (nap), and 22 psoriatic arthritis (pa) patients. a complement fixation test was used with yersinia enterocolitica 0:3 type (yec), yersinia pseudotuberculosis (ypt), campylobacter jejuni (cj), and campylobacter fetus (cf) antigens; the detection of anti-chlamydia tracho ...19921458698
nosocomial meningitis due to campylobacter fetus subspecies fetus in a neonatal intensive care unit.we experienced a nosocomial outbreak of campylobacter fetus subspecies fetus (c. fetus) meningitis in a neonatal intensive care unit. a cluster of three infants developed meningitis approximately 1-1.5 months after the index patient was admitted. two asymptomatic intestinal carriers of c. fetus were detected during the outbreak. our experience indicates that c. fetus can cause nosocomial meningitis in neonates, and asymptomatic carriers may play a role in the transmission of the organism.19921442025
specific detection of campylobacter jejuni and campylobacter coli by using polymerase chain reaction.development of a routine detection assay for campylobacter jejuni and campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (pcr). an oligonucleotide primer pair from a conserved 5' region of the flaa gene of c. coli vc167 was used to amplify a 450-bp region by pcr. the primer pair specifically detected 4 strains of c. coli and 47 strains of c. jejuni; but it did not detect strains of campylobacter fetus, campylobacter lari, campylobacter upsaliensis, cam ...19921400961
nucleotide sequence analysis of the gene encoding the caulobacter crescentus paracrystalline surface layer protein.the entire nucleotide sequence of the rsaa gene, encoding the paracrystalline surface (s) layer protein (rsaa) of caulobacter crescentus cb15a, was determined. the rsaa gene encoded a protein of 1026 amino acids, with a predicted molecular weight of 98,132. protease cleavage of mature rsaa protein and amino acid sequencing of retrievable peptides yielded two peptides: one aligned with a region approximately two-thirds the way into the predicted amino acid sequence and the second peptide correspo ...19921393820
differentiation of the subspecies of campylobacter fetus by genomic sizing.campylobacter fetus subsp. fetus and c. fetus subsp. venerealis are currently differentiated by tolerance to glycine and by their epidemiology. analysis of c. fetus dna by pulsed-field gel electrophoresis, after digestion with the restriction endonucleases smai and sali, was used to differentiate between the subspecies. all strains presently identified as c. fetus subsp. fetus had a genomic size of 1.1 mb, whereas the majority of the c. fetus subsp. venerealis strains had a genomic size of 1.3 m ...19921354478
[neonatal campylobacter fetus septicemia: a case secondary to maternal-fetal contamination].a favourable outcome of a severe cambylobacter fetus sepsis in a neonate is reported. the transmission was probably vertical. his mother experienced diarrhoea 15 days before birth. no infecting organism was isolated from maternal stools, but maternal antibody response was significant. such a perinatal infection with meningitis and septicemia is uncommon. bacteriological diagnosis requires an enriched medium and a long incubation. the diagnosis should be evoked in view of an anamnestic context, a ...19921319039
[evaluation of antimicrobial drugs and atmospheres for the isolation of campylobacter fetus subspp. from the bovine genital tract].growth of 3 reference bovine c. fetus strains in media with and without antibiotics and bacteriostats active against the most common contaminant bacteria in the bovine genital tract was evaluated. in addition, 2 regional bovine c. fetus strains and 1 reference c. sputorum biovar bubulus strain were used in some experiments. reference strain c. fetus subsp. venerealis was completely inhibited by polymyxin (> or = 0.25 iu/ml) whereas the other c. fetus strains were not inhibited. in shepler's medi ...19921302865
ovine campylobacterosis: preliminary studies of the efficacy of the in vitro serum bactericidal test as an assay for the potency of campylobacter (vibrio) fetus subsp intestinalis in vitro serum bactericidal test was developed to assess the efficacy of campylobacter fetus bacterins. four experimental monovalent bacterins (either serotype c or a-2) and 2 commercial bivalent bacterins (a "suspect" and an "efficacious" bacterin) with aluminum hydroxide adjuvant were administered to sheep and rabbits from which antiserums were then prepared. the different vaccines were evaluated by comparing the in vitro bactericidal activity of the sheep and rabbit antiserums. results of ...19761267237
cystine aminopeptidase activity (oxytocinase) in pregnant guinea pigs: normal and infected with campylobacter fetus.the cystine aminopeptidase (cap) activity was determined in plasma from guinea pigs at various stages of gestation and in pregnant guinea pigs infected with campylobacter fetus subspecies intestinalis. the cap activity decreased with length of gestation, which is in contrast to the changes in activity in persons. infected guinea pigs near the time of abortion can be distinguished from normal guinea pigs on the basis of plasma cap activity, if the plasma samples are taken 1 to 2 days before abort ...19761259230
studies on veneral transmission of campylobacter fetus by immunised bulls.temporary contamination of the penis and prepuce with campylobacter fetus subsp. venerealis and campylobacter fetus subsp. venerealis biotype intermedius may occur when immunised bulls mate with infected heifers. however, 2 experiments showed that this contamination was not a significant cause of genital infection in susceptible heifers that subsequently mated with bulls.19751243730
vibrio fetus infection. 19751235756
immunisation of cattle against vibriosis with vaccines prepared from campylobacter fetus subsp fetus.subcutaneous administration of vaccines prepared from cells of campylobacter fetus subsp fetus strain a28 to heifers gave substantial protection against infertility due to c. fetus subsp venerealis strain b6. strains a28 and b6 had different heat-stable antigens and conformed respectively to serotype b and serotype a of berg et al (1971). the results suggested that the protective antigens were heat-labile antigens common to both strains. although vaccines prepared from serotype b strains of c. f ...19751180771
bovine venereal vibriosis: activity of inflammatory cells in protective immunity.the capability of bovine polymorphonuclear (pmn) and mononuclear phagocytes to kill campylobacter (vibrio) fetus venerealis was tested under various conditions in order to judge their roles in protective immunity. bovine pmn killed c fetus in the presence of immunoglobulin g (igg) but not immunoglobulin a (iga) antibodies. some phagocytosis occurred in the absence of antibody in glass-adherent cultures, but not in suspension cultures--indicating that surface phagocytosis by the neutrophils norma ...19751173209
the influence of age on the susceptibility of bulls to campylobacter fetus subsp venerealis.observations were carried out on 22 hereford bulls to determine the age at which they bacame carriers of c. fetus. during preliminary investigations, 5 bulbs were repeatedly exposed to the organism by artificial or natural means from the age of 18 months. all animals became longterm carriers on reaching an age of between 40 and 70 months. prior to this, a temporary carrier state lasting from 4 to 29 days was produced in 4 bulls. in a subsequent experiment, 17 bulls of 3 different age groups were ...19751167135
perinatal lamb mortality in western australia: 5. vibrionic infection.vibrionic abortion was diagnosed in sheep on 48 occasions between 1963-65 and 3 of these were associated with other major abortion pathogens. the outbreaks were scattered throughout the agricultural area of western australia. time-of-death of 91 infected lambs was: ante-parturient death 50.5%, parturient death 13.2% and post-parturient death 36.3% (deaths occurring immediately after death 28.6%, deaths delayed after death 2.2%, and late post-parturient death 5.5%). the majority of lambs had gros ...19751164266
effects of mycoplasma spp, trichomonas fetus, and campylobacter fetus on ciliary activity of bovine uterine tube organ cultures.microscopic observations were made of sections of normal bovine uterine tube (oviduct) and fimbriae maintained in vitro and exposed to agents known to localize in the bovine genital tract, i.e., mycoplasma, trichomonas fetus, and campylobacter fetus (vibrio fetus). only c fetus stopped ciliary activity. scanning electron microscopy (sem) revealed loss of most cilia. sterile filtrate of c fetus culture had no effect.19751155827
vibrio fetus septicemia in colorado. 19751145030
campylobacter fetus infection in humans. analysis of three cases. 19751133862
vaccination against vibrio (campylobacter) fetus infection in sheep in late pregnancy.a single injection of vaccine containing disrupted formol-killed vibrio (campylobacter) fetus var intestinalis serotype v was shown to give good protection against abortion from an oral challenge of the same serotype administered simultaneously. protection was also afforded by vaccination at the time of the first abortion in sheep infected orally one month previously. these results suggest that vaccination subsequent to an abortion might be of value in preventing a proportion of further abortion ...19751119063
bovine veneral vibriosis: cure of genital infection in females by systemic immunization.cure of female cattle with venereal vibriosis by systemic immunization with killed campylobacter fetus cells in incomplete freund adjuvant was investigated. heifers infected in the cervicovaginal area with a cloned population of c. fetus venerealis were vaccinated subcutaneously 14 and 24 days thereafter with the infecting strain in incomplete freund adjuvant. six of eight vaccinated heifers were free of infection 25 to 48 days postinfection. one of the cured animals had an intercurrent infectio ...19751112614
bilateral deep brachial vein thrombophlebitis due to vibrio fetus.a patient had bilateral deep brachial vein thrombophlebitis in which vibrio fetus was recovered from six blood cultures of the six drawn. fever and phlebitis continued with treatment with intravenous doses of heparin and oxacillin but rapidly improved with treatment change of oxacillin to clindamycin. in vitro antibiotic disk susceptibility testing confirmed resistance to oxacillin and susceptibility to clindamycin. vibrio fetus infection is associated with the vascular endothelium in this and p ...19751041482
[the bacteriological diagnosis of vibrio fetus infection in man (author's transl)].a synopsis of the present classification of vibrio fetus is given (tab. 1, 2). besides the presentation of the importance of these bacteria in veterinary medicine, 97 human infections have been published up to now. the geographic, age and sex distribution of these cases and the material out of which the pathogens were isolated is presented (tab. 3, 4). the clinical picture is summarized. for the culture of vibrio fetus liver broth as described by tarozzi incubated for at least 3-4 days was suita ...19751041142
vibrio fetus endocarditis in a patient with systemic lupus erythematosus.vibrio fetus endocarditis occurred in a patient with systemic lupus erythematosus receiving azathoprin and prednisone. blood cultures required 14 days to become positive. the fastidious growth requirement of this organism is reviewed because lack of appreciation of these may result in failure to make the diagnosis. this is the first reported case of vibrio fetus endocarditis occurring in the setting of a connective tissue disorder and immunosuppressive therapy.19761015513
a simple technique for mass cultivation of campylobacter fetus.studies using 86 media for maximum growth of campylobacter fetus for antigen production showed that a diphasic medium (solid base with liquid overlay) was most suitable. the solid base was double strength cystine heart agar. the liquid overlay was thioglycollate medium of brewer (135-c) without agar. this medium yielded maximum growth of c. fetus in six days with good motility, less clumping and less filament formation than all other media tried.19761000394
nature of antibodies to campylobacter fetus in preputial secretions from a vaccinated bull. 1976990892
immunisation against bovine vibriosis due to campylobacter fetus subsp. fetus biotype intermedius.a vaccine prepared from campylobacter fetus subsp. fetus biotype venerealis gave only partial protection to heifers and bulls against genital infection with c. fetus subsp. fetus biotype intermedius. heifers and bulls were fully immunised against c. fetus subsp. venerealis biotype intermedius by administration of a vaccine prepared from homologous organisms.1976985256
septicaemia and meningitis with campylobacter fetus subspecies intestinalis.two cases of infection with campylobacter fetus subspecies intestinalis are reported. in a 46-year-old farmer the development of septicaemic campylobacteriosis with acute gastroenterocolitis was favoured by malnutrition due to chronic alcoholism. after treatment with erythromycin the patient recovered completely. the second patient, a 40-year-old worker with meningitis, recovered after combined streptomycin-erytro-mycin therapy. in both patients antibodies against homologous and reference strain ...1976947847
stimulatory effect of dihydroxyphenyl compounds on the aerotolerance of spirillum volutans and campylobacter fetus subspecies jejuni.the aerotolerance of the microaerophilic bacterium spirillum volutans was greatly stimulated in a defined medium by the presence of dihydroxyphenyl ferric iron-binding compounds such as nor-epinephrine at 10(-5) to 10(-6) m. dihydroxyphenyl compounds at 2 x 10(-4) m, or iron salts (ferrous or ferric) at high concentration, greatly increased the aerotolerance of a strain of campylobacter fetus subsp. jejuni when grown on streak plates of brucella agar. the results suggest that the microaerophilis ...1976942201
[epidemiologic studies on the vibrio fetus infection of cattle]. 1976938446
[biochemical and serological investigations of flagellae of campylobacter fetus (author's transl)].flagellae of campylobacter fetus group o, types 1, 2 and 7 were prepared. first they were separated from cell bodies using an ultramix. the suspension was then centrifuged for 20 mins. at 10000 rpm and the supernatant frozen at -40 degrees c. this is a simple method for the enrichment of preparations of flagellae, as they become tangled up and accumulate in the inferior third part of the frozen liquid. the physicochemical basis of this phenomenon was discussed. after thawing and spinning for 20 ...1976936824
an abattoir survey of bull genitalia.this paper describes a survey of 504 bull genitalia collected after slaughter at 3 western australian abattoirs over 18 months. details of the gross pathology, histopathology, and microbiology of the genitalia, and physical dimensions of the testicles are presented. the major breeds represented were hereford (30%), shorthorn (16%), friesian (14.3%), and aberdeen angus (13.9%). the mean age of 263 bulls (52.6%) was 4.4 years. the mean length and breadth of 469 paired testicles was 10.8 cm and 6.9 ...1977901333
campylobacter fetus septicemia and hepatitis in a child with agammaglobulinemia. 1977894415
campylobacter fetus septicemia with concurrent salpingitis.campylobacter fetus was isolated from the blood of a nonpregnant young woman who had a clinical diagnosis of salpingitis. a description of the isolate of c. fetus is given. an increasing awareness of human infections and capability of growing this microaerophilic organism should contribute to our understanding of the incidence and virulence of this bacterium.1977886009
septicemia due to campylobacter fetus in a newborn infant with gastroenteritis. 1977842576
nature of campylobacter fetus agglutinins in vaginal mucus from experimentally infected heifers. 1977837209
campylobacter fetus associated with pulmonary abscess and unusual case of community-acquired necrotizing aspiration pneumonia with pulmonary abscess and empyema associated with campylobacter fetus (formerly vibrio fetus) in mixed culture is reported. this microaerophilic gram-negative curved bacillus has been increasingly associated with human disease. unlike most pathogens associated with community-acquired aspiration pneumonia, this organism is usually resistant to penicillin. infection associated with this organism is characteristically relapsing ...1977830489
antibody-mediated immobilization of campylobacter fetus: inhibition by a somatic antigen.immobilization tests were conducted on a wild-type strain of campylobacter fetus subsp. intestinalis and on a mutant lacking an antiphagocytic cell surface component. highly effective immobilization of the mutant, both as single cells and clumps of cells, was produced with an antiserum containing antibodies specific for the flagellar hook and filament and for the o antigen. damage to flagellar hooks after reaction with this antiserum was observed only with cells of the mutant. single-cell immob ...1976819375
major outer membrane protein of campylobacter fetus: physical and immunological characterization.the outer membrane proteins of campylobacter fetus have been isolated by extraction of cell envelopes both in triton x-100 and ethylenediaminetetraacetate (edta) and in sodium dodecyl sulfate (sds). each method yielded a major protein component, which migrated identically in sds-polyacrylamide gel electrophoresis (sds-page) and proved comparable in electrophoretic and molecular weight characteristics to the analagous protein from escherichia coli. in addition, the surface of sds-extracted c. fet ...1976819374
infections associated with ovine perinatal mortality in victoria.the occurrence of infections associated with perinatal mortality was investigated. in 1970, carcasses from 94 flocks distributed throughout victoria were examined by microbiological and histopathological techniques. carcasses from 4 of the same flocks were similarly examined in 1971. widespread occurrence of listeria monocytogenes and campylobacter fetus was demonstrated with l. monocytogenes being recognised in 25% of the flocks examined. no other bacterial pathogens commonly associated with ov ...1975809028
in-vitro effect of edta-tris-lysozyme solutions on selected pathogenic bacteria.the in-vitro effect of edta-tris-lysozyme solution on 16 pathogenic bacteria of medical or veterinary importance was determined. marked decreases in bacterial count occurred with pseudomonas aeruginosa, escherichia coli, moraxella osloensis and campylobacter fetus, and smaller decreses with salmonella typhimurium, shigella boydii, aeromonas hydrophila, proteus mirabilis, listeria monocytogenes and erysipelothrix insidiosa. the test solution had no effect on klebsiella ozaenae, brucella canis, co ...1975805241
flagellar ultrastructure and flagella-associated antigens of campylobacter fetus.ultrastructural examinations of the flagellum of compylobacter (vibrio) fetus were performed throughout the growth cycle. filament diameters, exceeding 17.6 nm during the exponential phase, were substantially greater than those reported for unsheathed flagella of other genera with the exception of pseudomonas fluorescens. filament diameters increased during growth, reaching a mean width of 21.2 nm in middle to late stationary phase. internal flagellar structure, principally of the parallel lined ...1975804471
[isolation of campylobacter fetus in two herds of sheep in sweden (author's transl)].in two herds of sheep with numerous abortions campylobacter fetus subspecies intestinalis was isolated from fetuses, fetal membranes, and faecal specimens. in herd a, with good hygiene, 12 ewes out of 250 aborted. in herd b, with poor hygiene, 54 out of 171 ewes aborted and 14 of them died; for a further 9 ewes the lambs died postnatally. the clinical course in both herds shows that proper supervision and possibilities of prompt isolation of aborting ewes can substantially reduce the spread of i ...1976790309
spontaneous peritonitis of cirrhosis due to campylobacter unusual case of spontaneous peritonitis of cirrhosis associated with campylobacter fetus is reported. this microaerophilic gram-negative bacillus has been increasingly associated with human disease recently, particularly in patients with cirrhosis. the infection is characteristically relapsing in nature and requires protracted antimicrobial therapy.1976780182
[type differentiation of v. fetus and v. bubulus by using fluorescent antisera].obtained were type-specific fluorescent vibrionic antisera for the laboratory identification of vibrio fetus strains. they produced positive fluorescence only with the homologous strains, and did not react with the heterologous strains. this showed that the sera were strongly specific. their use is suggested for the differentiation typing of the vibrio strains.1975766378
campylobacter fetus infection in children. 1979762641
infection of humans with campylobacter fetus. 1978737615
campylobacter infections. first reports from red cross war memorial children's hospital, cape town.we report 10 cases of campylobacter fetus infection, isolated during a 7-month period, which were all sensitive to erythromycin. the aetiology and epidemiology are discussed.1978734547
isolation of campylobacter fetus subspecies jejuni. 1978732739
microcapsule of campylobacter fetus: chemical and physical characterization.the antiphagocytic antigen (antigen [a]) comprising the microcapsule of a strain of campylobacter fetus subsp. intestinalis has been purified from culture supernatants by ammonium sulfate fractionation and free-flow electrophoresis. antigen [a] is a glycoprotein containing about 4% carbohydrate consisting of hexose, pentose, and methylpentose. the composition of the protein was typical of bacterial extramural structural proteins in its low content of basic, aromatic, and sulfur-containing amino ...1978730387
susceptibility of campylobacter fetus subsp. jejuni to twenty-nine antimicrobial agents.the activity of 29 antimicrobial agents was tested against 95 strains of campylobacter fetus subsp. jejuni isolated from human stools. furazolidone and gentamycin were the most active agents. the tetracyclines, erythromycin, and chloramphenicol were very active against most of the strains, but with each antibiotic a few resistant strains were found. the penicillins were relatively inactive, and the cephalosporins tested were only active against occasional strains.1978718153
elimination of genital vibriosis in female cattle by systemic immunization with killed cells or cell-free extracts of campylobacter fetus. 1978712110
evaluation of a transport medium for campylobacter (vibrio) fetus.a quantitative assessment was performed on a serum enrichment medium for campylobacter (vibrio) fetus, to test its effectiveness as a transport medium. abundant yields of c fetus were obtained with an inoculum size of 10(4), even after keeping the medium in air at 22 to 23 c for 4 days prior to incubation at 37 c under microaerophilic conditions. the organism could be isolated from the medium kept in air at 22 to 23 c for at least 2 days, with an inoculum as small as 100 organisms. it was conclu ...1978711588
effect of interval between booster vaccination and time of breeding on protection against campylobacteriosis (vibriosis) in cattle.thirteen heifers (group a) given a booster vaccination with campylobacter fetus 4 1/2 months before breeding to bulls infected with the homologous strain had a pregnancy rate of 54%, compared with a pregnancy rate of 92% for 12 heifers (group b) given a booster vaccination 10 days before the 63-day breeding period began. none of 7 nonvaccinated heifers became pregnant, and all remained infected for at least 136 days. all three groups of heifers were bred by the same infected bulls. nine of the h ...1978711587
campylobacteriosis in man: pathogenic mechanisms and review of 91 bloodstream infections.five patients with campylobacter fetus(previously called "vibrio fetus") bacteremia are presented with enteric symptoms in four patients, a self-limited course in three, and with possible nosocomial infection in one patient who had disseminated malignancy. the clinical syndromes of 91 bacteremic patients with campylobacteriosis and c. fetus taxonomy and pathogenicity are reviewed. studies of potential pathogenic mechanisms in enteric infections failed to reveal the production of either heat-stab ...1978707518
gram-negative endocarditis caused by campylobacter fetus.campylobacter fetus is a rare cause of endocarditis that is difficult to diagnose because of the fastidiousness of the organism. the source of infection is unknown and it may involve a normal or previously damaged aortic valve. the onset is insidious, and if there is a prior history of recent onset of fever, thrombophlebitis, and diarrhea, infection caused by this organism should be considered. echocardiography may be useful. the organism is sensitive to a variety of antibiotics, including the c ...1978705408
isolation of campylobacter fetus from bulls. 1978687292
the serological response of sheep to a trivalent campylobacter (vibrio) fetus var intestinalis vaccine. 1978676003
improved media for growth and aerotolerance of campylobacter fetus.the microaerophilic nature of campylobacter fetus has complicated its recovery from human and animal sources. in this study, modifications of brucella agar and broth were tested for enhancement of growth and aerotolerance of 64 strains of c. fetus, representing each subspecies. brucella agar supplemented with 0.025% each feso4 7h2o, sodium metabisulfite, and sodium pyruvate, supported growth of 98, 77, and 63% of the strains at 6% o2, 17% o2, and 21% o2, respectively. unsupplemented brucella aga ...1978670386
Displaying items 1701 - 1800 of 1841