PMID(sorted descending)
colonization of gastrointestinal tracts of chicks by campylobacter jejuni.bacterial enumeration and histologic examination of organs and tissues of 8-day-old chicks 7 days after peroral inoculation with campylobacter jejuni revealed that the organism colonized primarily the lower gastrointestinal tract. the principal sites of localization were the ceca, large intestine, and cloaca, where densely packed cells of c. jejuni were observed in mucus within crypts. examination of c. jejuni-colonized crypts by transmission electron microscopy revealed that the campylobacters ...19883060015
two-year survey of etiologic agents of diarrheal disease at san lazaro hospital, manila, republic of the philippines.the prevalence of bacterial pathogens and rotavirus in 2,908 patients with diarrhea who were admitted to san lazaro hospital in manila in 1983 and 1984 was determined. one or more enteric pathogens were isolated or detected in samples from 1,698 (58.4%) patients. isolation rates for the various enteropathogens were as follows: rotavirus, 30.6%; shigella spp., 11.6%; salmonella spp., 9.2%; enterotoxigenic escherichia coli (1983 only), 7.8%; vibrio cholerae biotype eltor, 3.8%; non-o1 v. cholerae, ...19873038946
antimicrobial resistance of campylobacter jejuni and campylobacter coli with special reference to plasmid profiles of japanese clinical isolates.a total of 111 clinical isolates of campylobacter jejuni and 10 clinical isolates of campylobacter coli were characterized by their susceptibility to nine antimicrobial agents and by their plasmid profiles on agarose gel electrophoresis. all of the c. jejuni isolates were susceptible to chloramphenicol, ciprofloxacin, erythromycin, kanamycin, and nalidixic acid, but 55% were tetracycline resistant. in the 10 c. coli isolates, a high prevalence of multiple-antibiotic resistance was noted. plasmid ...19873038005
characterization and expression of a cloned tetracycline resistance determinant from campylobacter jejuni plasmid pua466.a tetracycline resistance (tcr) determinant previously cloned from the campylobacter jejuni plasmid pua466 (d. e. taylor, j. bacteriol. 165:1037-1039, 1986) was localized by restriction endonuclease mapping, subcloning, and tn1000 insertion mutagenesis to a 2-kilobase region consisting of 1.8- and 0.2-kilobase hincii fragments. tcr encoded by the cloned fragment (puoa1) was expressed constitutively in escherichia coli. a protein with an apparent molecular weight of 68,000 encoded by puoa1 was pr ...19873036765
genetic studies of kanamycin resistance in campylobacter jejuni.campylobacter jejuni 3h40 and 4b20 harbored 59-kilobase (kb) self-transmissible plasmids encoding resistance to kanamycin and tetracycline. although the two antibiotic resistances were more frequently inherited together, some transconjugants and ethidium bromide segregants which were resistant to only one of these antibiotics were recovered. the kanamycin-susceptible, tetracycline-resistant segregants carried plasmids 4 kb smaller than the 59-kb plasmids of their parents, whereas the kanamycin-r ...19863021049
comparison between children treated at home and those requiring hospital admission for rotavirus and other enteric pathogens associated with acute diarrhea in melbourne, australia.the etiology of acute diarrhea in children less than 42 months of age attending one pediatric hospital in melbourne, australia, was studied during a 7-month period encompassing the winter of 1984. pathogens identified in 157 children treated as outpatients with mild disease were compared with those in 232 children hospitalized with severe disease. the pathogens (and frequencies among outpatients and inpatients, respectively) detected were rotaviruses (32.5 and 50.9%), enteric adenoviruses (8.9 a ...19863020082
cytotoxic and cytotonic factors produced by campylobacter jejuni, campylobacter coli, and campylobacter laridis.complete toxigenicity studies were performed on 341 strains of campylobacter spp., including 23 nonhuman isolates. toxin profiles based on both cytotonic and cytotoxic factors were determined after analyzing responses in vero, hela, cho and y-1 cells. suckling mouse assays were consistently negative for all culture filtrates tested. toxin-producing strains were frequently encountered among both the human and nonhuman strains of campylobacter jejuni, c. coli, and c. laridis investigated. strains ...19863018039
an epidemiological study of selected calf pathogens on holstein dairy farms in southwestern ontario.fecal samples from calves on 78 randomly selected holstein dairy farms in southwestern ontario were screened for salmonella, campylobacter jejuni/coli, enteropathogenic escherichia coli, rotavirus and coronavirus. based on the observed prevalence, 22% of farms had calves infected with salmonella, 13% with campylobacter jejuni/coli, 41% with enteropathogenic e. coli, 19% with rotavirus and 5% with coronavirus. these estimates can be modified, using a method developed by mullen and prost (1983) fo ...19863017528
plasmid-mediated tetracycline resistance in campylobacter jejuni: expression in escherichia coli and identification of homology with streptococcal class m determinant.plasmid pua466, a 45-kilobase transmissible tetracycline resistance plasmid from campylobacter jejuni was mapped with avai, avaii, bcli, hincii, psti, xhoi, and xbai. the resistance determinant was cloned and expressed in escherichia coli and was homologous with a class m determinant from streptococcus spp.19863005233
participation of cytochromes in some oxidation-reduction systems in campylobacter fetus.campylobacter species are rich in c-type cytochromes, including forms which bind carbon monoxide. the role of the various forms of cytochromes in campylobacter fetus has been examined in cell-free preparations by using physiological electron donor and acceptor systems. under anaerobic conditions, nadph reduced essentially all of the cytochrome c in crude cell extracts, whereas the reduction level with succinate was 50 to 60%. the carbon monoxide spectrum with nadph was predominated by the cytoch ...19852995315
in vitro susceptibility of campylobacter jejuni to 27 antimicrobial agents and various combinations of beta-lactams with clavulanic acid or sulbactam.the in vitro susceptibility of human isolates of campylobacter jejuni was investigated with 27 antibiotics and 8 combinations of beta-lactams with clavulanic acid or sulbactam. ansamycin, the new quinolines, erythromycin, and cefpirome were the most active drugs against c. jejuni; amoxicillin, ampicillin, cefotaxime, and ceftazidime 90% of the isolates, greater than or equal to 50 mg/liter). the activity of various beta-lactams was unchanged by the addition of clavulanic acid or sulbactam.19852994557
dna relatedness and biochemical features of campylobacter spp. isolated in central and south australia.investigations of the etiology of diarrhea in patients in south australia and the northern territory showed that campylobacter spp. other than campylobacter jejuni and c. coli were common in children. campylobacters which were hippurate positive, nitrate negative, and susceptible to cephalothin and polymyxins were shown to be closely related to c. jejuni by dna studies. thermotolerant catalase-negative campylobacters were also isolated. these were h2s negative and biochemically resembled the cat ...19852991331
a two-year longitudinal study on the etiology of acute diarrhea in young children in northern italy.during a 24-month period, 561 young children (6 months-2 years of age) hospitalized for acute diarrhea were studied for enteric pathogens. patients positive for one or more pathogens were 359 (64.0%). infection with one pathogen was found in 266 (47.4%) patients, whereas multiple infection was detected in 93 (16.6%) patients. enteropathogens associated with disease were rotaviruses: 150 (26.7%), adenoviruses: 99 (17,6%), non-polio enteroviruses: 48 (8.5%), coronaviruses: 10 (1.8%), parvoviruses: ...19852983179
the effect of campylobacter lipopolysaccharide on fetal development in the mouse.purified lipopolysaccharide (lps) obtained from isolates of campylobacter fetus ss. fetus and campylobacter jejuni impaired fetal development when administered to mice on day 13 of pregnancy. strikingly more fetal resorption was produced by c. jejuni lps than by similar amounts of c. fetus ss. fetus lps. three of the four campylobacter strains examined produced lps that had no effect on maternal health, but lps from one c. jejuni strain killed all of the mice to which it was administered.19882968457
campylobacters and impaired fetal development in mice.intravenous injection of eight human strains of campylobacter fetus ss fetus and campylobacter jejuni into mice at various stages of pregnancy demonstrated significant strain differences in ability to affect implantation of the fertilised ovum and to cause resorption of the mouse fetus. implantation was significantly impaired when c. fetus ss fetus was injected intravenously on day 2 of pregnancy, but no effect was observed in mice receiving c. jejuni. on day 6 of pregnancy, before the developme ...19882961890
comparative in vitro activity of a-56268 (te-031) against gram-positive and gram-negative bacteria and chlamydia trachomatis.the in vitro activity of a-56268 (te-031) was determined and compared with that of 13 antibiotics against 401 gram-positive and gram-negative bacteria and 11 strains of chlamydia trachomatis. a-56268 was very active against methicillin-susceptible staphylococcus aureus and neisseria gonorrhoeae, and was among the most active of the agents tested against listeria monocytogenes, streptococci and chlamydia trachomatis. it was moderately active against haemophilus spp., vibrio spp., campylobacter je ...19872954818
in vitro activity of a-56268 (te-031), a new macrolide, compared with that of erythromycin and clindamycin against selected gram-positive and gram-negative organisms.the in vitro activity of a-56268 was determined and compared with that of erythromycin and clindamycin against a limited spectrum of 401 gram-positive and gram-negative organisms. a-56268 was quite active against erythromycin-susceptible staphylococcus aureus, neisseria gonorrhoeae, listeria monocytogenes, streptococcus pneumoniae, streptococcus pyogenes, and group b streptococci and was moderately active against campylobacter fetus subsp. fetus. a-56268 was consistently bactericidal only for s. ...19872952063
influence of iron on growth, morphology, outer membrane protein composition, and synthesis of siderophores in campylobacter jejuni.three human isolates of campylobacter jejuni were grown in a biphasic culture medium with and without the addition of a synthetic chelator to induce iron limitation. cells grown in low-iron medium exhibited slower growth rates and altered cellular morphology. increased numbers of longer, more filamentous forms were seen in gram-stained smears. three proteins, with apparent mrs of 82,000, 76,000, and 74,000, were consistently present in the outer membrane of cells grown in low-iron medium. at lea ...19862944843
[antibiotic resistance and distribution of plasmid-encoded beta- lactamases among agents of traveller's diarrhea].the polymicrobial aetiology of travellers's diarrhoea in 356 tourists travelling in thailand and burma was investigated. besides enterotoxigenic e. coli, salmonella sp. and campylobacter fetus ssp. jejuni were identified as the most important enteric pathogens. minimal inhibitory concentrations of several commonly used antibiotics were determined to reveal the percentage of enteric pathogens being resistant. 36.2% e. coli strains were found to be resistant to ampicillin and 14.3% of the campylob ...19862943094
[campylobacter fetus ssp. fetus endocarditis]. 19862942861
mucus colonization as a determinant of pathogenicity in intestinal infection by campylobacter jejuni: a mouse cecal model.human isolates of the intestinal pathogen campylobacter jejuni have been shown to colonize mucus on the outer surface and deep within the intestinal crypts of gnotobiotic or germfree mice. the cecal crypts are preferentially colonized. a model of mucus colonization by c. jejuni in the mouse cecum has been developed, using antibiotic- and magnesium sulfate-treated specific-pathogen-free animals. these spiral-shaped bacteria colonize the mucus in a similar manner to the normal spiral-shaped microb ...19862935499
[meningitis caused by campylobacter fetus fetus. a case report]. 19852931701
campylobacteriosis in an aborted equine fetus.abortion caused by campylobacter fetus subsp fetus was diagnosed in a 7-month-old equine fetus. the fetus was small for its gestational age. macroscopically, the proximal portion of the small intestine was hemorrhagic and its wall was thick. histologically, the brunner glands were distended with neutrophils, and the submucosa was thick, owing to fluid accumulation and/or cellular infiltrates. curved bacteria were observed in the brunner glands and intestinal glands. campylobacter fetus subsp fet ...19892917894
campylobacter jejuni and campylobacter coli diarrhoea in rural and urban populations in yugoslavia.during a 4-month period during the summer of 1985, campylobacters were isolated from 338 (16.3%) of 2080 patients with acute diarrhoea attending the university hospital of infectious diseases in zagreb. of these isolates 220 (64.1%) were campylobacter jejuni and 118 (34.9%) were c. coli. the patients were drawn from three residential zones in and around zagreb: inner city, peripheral city and rural. incidences of campylobacter diarrhoea ranged from 71 per 100,000 per year in inner city residents ...19892917618
campylobacter jejuni isolations from mexican and swedish patients, with repeated symptomatic and/or asymptomatic diarrhoea episodes.the presence of different campylobacter jejuni serotypes in swedish patients with diarrhoea and in mexican patients with or without diarrhoea was investigated with special reference to repeated isolations during the course of infection and to symptomatic and asymptomatic episodes. the study included 136 c. jejuni isolates from 62 mexican children and 173 isolates from 68 swedish patients. the bacteria were serotyped for heat-stable (hs) and heat-labile (hl) antigen. swedish patients, all with sy ...19892917617
substrate utilization by campylobacter jejuni and campylobacter attempt was made to elucidate in campylobacter spp. some of the physiologic characteristics that are reflected in the kinetics of co2 formation from four 14c-labeled substrates. campylobacter jejuni and c. coli were grown in a biphasic medium, and highly motile spiral cells were harvested at 12 h. of the media evaluated for use in the metabolic tests, minimal essential medium without glutamine, diluted with an equal volume of potassium sodium phosphate buffer (ph 7.2), provided the greatest s ...19862877615
identification and characterization of two campylobacter jejuni adhesins for cellular and mucous substrates.campylobacter jejuni is able to colonize the human intestinal mucosa and cause disease. for this reason, it was important to investigate mechanisms by which c. jejuni adheres to epithelial cells and intestinal mucus gel. all strains of c. jejuni used were able to adhere to int 407 epithelial cells and mucus, but high adherence to one substrate did not necessarily indicate comparable adherence to the other. the adherence of c. jejuni to cells was inhibited partially by treating the bacterial cell ...19862873103
subdural empyema caused by campylobacter fetus ssp. fetus. 19862871115
gene disruption and replacement as a feasible approach for mutagenesis of campylobacter jejuni.campylobacter jejuni and campylobacter coli are important causes of human enteric infections. several determinants of pathogenicity have been proposed based on the clinical features of diarrheal disease and on the phenotypic properties of campylobacter strains. to facilitate an understanding of the genetic determinants of campylobacter virulence, we have developed a method for constructing c. jejuni mutants by shuttle mutagenesis. in the example described here, a kanamycin resistance gene was in ...19882832375
cloning and expression of a tetracycline resistance determinant from campylobacter jejuni in escherichia coli.the tetracycline resistance gene (tet) from the campylobacter jejuni plasmid pfkt1025 was cloned into both puc18 and pbr322 and was expressed when the chimeric plasmids were introduced into escherichia coli. the location of the tet determinant on the chimeric plasmids was determined by bal 31 deletion mapping within a 2.25-kilobase (kb) rsai-hincii fragment. a protein of approximately 70 kilodaltons was consistently produced by e. coli maxicells harboring the cloned tet determinant. a 500-base-p ...19872823694
gene transfer from escherichia coli to campylobacter species: development of shuttle vectors for genetic analysis of campylobacter jejuni.a shuttle cloning vector (pil550) has been constructed which can be mobilized from escherichia coli to campylobacter jejuni, campylobacter coli, and campylobacter fetus by complementation with the transfer functions of an incp plasmid in trans, with a frequency of 10(-4) transconjugants per donor. we also present evidence for a dna modification system in c. jejuni.19872822671
an examination of campylobacter fetus subsp. fetus by restriction endonuclease analysis and serology.restriction endonuclease analysis was used to examine 70 different strains of campylobacter fetus subsp. fetus, which were isolated from aborted sheep foetuses. the strains could be divided into seven types based on the dna fragment patterns obtained by electrophoresis after digestion with the enzyme bsteii. with one exception, digestion with the enzyme xhoi allowed the strains to be grouped identically to that for bsteii. antisera were made against formalized whole cells representative of each ...19872820112
early treatment of campylobacter jejuni enteritis.the bacteriologic and clinical effects of early antibiotic treatment of campylobacter jejuni enteritis were studied. erythromycin rapidly eliminated c. jejuni from stools, whereas trimethoprim-sulfamethoxazole did not. despite its bacteriologic effectiveness, erythromycin did not reduce the duration or severity of diarrhea, abdominal pain, or other symptoms.19892818711
campylobacter fetus cholangitis and bacteremia. 19892804850
in vitro evaluation of antibiotics for treatment of meningitis caused by campylobacter fetus subspecies fetus. 19892797963
in-vitro evaluation of antibiotic combinations against campylobacter fetus.we evaluated the in-vitro activity of 16 antibiotics against 22 clinical isolates of campylobacter fetus. the interaction of these antibiotics was also tested by chequer-board titration and time-kill assay. eight antibiotic combinations were evaluated. antagonism was not found with any of the combinations tested. the highest synergistic effect was obtained with a combination of ampicillin and cefazolin or ampicillin and gentamicin.19892793642
interaction of campylobacter species with antibody, complement and phagocytes.the opsonisation of four different campylobacter species for human neutrophils was studied using a chemiluminescence system and electron microscopy. opsonisation of campylobacter fetus, campylobacter coli, and campylobacter jejuni was mediated by antibody and enhanced by complement. antibody was not, however, required for the phagocytosis of campylobacter pylori because it activates the classical pathway of complement directly. this unusual property may be important in the pathogenesis of c pylo ...19892788111
waterborne outbreak of campylobacter enteritis after outdoors infantry drill in utti, finland.diarrhoea, abdominal pain, malaise and fever affected 75 of the 88 conscript soldiers in utti, finland after an outdoors infantry drill. campylobacter jejuni, heat-stable serotype 3/43/59, was isolated from 37 out of 63 men investigated. a clear serological response was evident in the risk group and negligible in the control group. the entire population at risk was interviewed. the outbreak was associated with the consumption of untreated surface water. c. jejuni, heat-stable serotype 3/43/59, w ...19892776848
effects of sample holding time, temperature, and atmosphere on the isolation of campylobacter jejuni from dogs.stool specimens were collected from 39 dogs, inoculated onto campylobacter blood agar plates, and divided into four subsamples. subsamples were held at 4 and 25 degrees c in room air and in a microaerobic environment and were reinoculated at 1, 2, 3, 4, 6, and 8 h. c. jejuni survived at least 3 h when it was held at 4 degrees c, but less than 2 h when it was held at 25 degrees c. the holding atmosphere was not associated with a difference in isolation rates.19892754014
virulence of campylobacter jejuni for chicken embryos.the pathogenicity of campylobacter jejuni was examined in chicken embryos. in this system, mortality data and histopathological findings induced by organisms and by bacterium-free filtered broth were identical. the absence in chicken embryo tissues both of organisms and of an inflammatory infiltrate suggests a toxin etiology.19892754005
enterotoxin production and serogroups of campylobacter jejuni and campylobacter coli from patients with diarrhea and from healthy laying hens.enterotoxin production, a possible virulence factor, was determined in campylobacter jejuni and campylobacter coli by two different techniques, the cho cell test and the gm1 enzyme-linked immunosorbent assay. the frequency of enterotoxigenic campylobacter strains was 32% in strains from both humans with acute enteritis and healthy laying hens, as measured by the cho cell test. the cho cell test was significantly more sensitive than the gm1 enzyme-linked immunosorbent assay in the detection of en ...19892754001
cases of infective endocarditis caused by campylobacter 18-year-old man with infective endocarditis caused by campylobacter fetus is reported. this bacterial species has long been of interest in veterinary medicine, and recently it has been reported to be one cause of infantile diarrhea. infective endocarditis arising from campylobacter fetus is rare. this is the fourth case reported in japan. the organism was resistant to several antibiotics, and large vegetations were found to involve the aortic valve. emergency excision of the infected aortic v ...19892748151
cellular fatty acid composition of campylobacter pylori from primates and ferrets compared with those of other campylobacters.the cellular fatty acid profiles of newly described campylobacters were determined on a polar, capillary column. six isolates of the gastric spiral organism, campylobacter pylori subsp. mustelae, from ferrets from australia, england, and the united states were all found to have a similar fatty acid profile which was different from that of c. pylori from humans; c. pylori subsp. mustelae did not have 3-hydroxyoctadecanoic acid (3-oh c18:0) and had much less tetradecanoic acid (c14:0) and much mor ...19892745703
identification of campylobacter jejuni and c. coli by gel electrophoresis of the outer membrane proteins.analysis of the electrophoretic profiles of the outer membrane proteins could be used to differentiate campylobacter jejuni (16 strains) from campylobacter coli (10 strains). this observation was confirmed by the study of dna homology obtained by a quantitative filter hybridization method. the hippurate hydrolysis test gave a poor correlation with the results of differentiation obtained by dna homology studies and outer membrane protein profile.19892745680
hexagonal surface layer of campylobacter fetus isolated from humans.electron microscopic studies of the surface structure of campylobacter fetus by the freeze-etching method showed two different types of s layer. one was in a hexagonal array, and the other was in a tetragonal array. a high-passage-number strain lost its s layer during cultivation on culture media but regained it after a single animal passage.19892744862
immune response to campylobacter jejuni and campylobacter coli in a cohort of children from birth to 2 years of age.a cohort of 111 children from bangui, central african republic, was surveyed for enteric campylobacter infections from birth to the age of 2 years; stools were examined biweekly in these children until 6 months of age and at least four times per year thereafter until 2 years of age and after each diarrheal episode. blood samples were obtained at birth and at 3, 6, 9, 12, 18, and 24 months of age. antibodies against glycine-extracted membrane antigens, purified flagella, and cholera toxin (ct) we ...19892744860
colonization of mice by campylobacter jejuni.both streptomycin-treated and untreated swiss white mice were irregularly colonized when challenged orogastrically with between 1 and 10(11) viable organisms of either of two strains of campylobacter jejuni. the organisms were occasionally recovered from portions of the intestinal tracts of these animals in numbers ranging from 10(1) to 10(3)/g when the challenge doses were 10(10) or more. when germfree mice were challenged with 10(8) organisms of either strain, the entire intestinal tracts of a ...19892744846
cellular events and intracellular survival of campylobacter jejuni during infection of hep-2 cells.invasion and intracellular survival of campylobacter jejuni in hep-2 cells were analyzed by transmission electron microscopy and by viable counts after killing of extracellular bacteria by gentamicin. during the first 30 min after challenge, no bacteria were seen in association with the host cell. after 1 h, campylobacters apparently attached to the cell membrane, with areas of close appositions. in these areas, an intracellular network of actin-like filaments was seen beneath the plasma membran ...19892731988
campylobacter fetus as a pulmonary pathogen in man. case report of post-pneumonectomy empyema. 19892710441
experimental campylobacter jejuni infection in macaca nemestrina.experimental infection of four specific-pathogen-free macaca nemestrina monkeys (aged 3.5 and 4.5 months) with campylobacter jejuni 81-176 caused acute diarrheal illness, characterized by fluid diarrhea, bloody stools, and fecal leukocytes, which lasted for approximately 7 to 11 days. histologic examination of intestinal biopsies showed acute colitis characterized by infiltration of the mucosa with neutrophils and lymphocytes, and cryptitis. there were no histologic changes in the small intestin ...19892707853
population genetics of human and animal enteric campylobacter strains.a total of 125 strains of campylobacter jejuni and campylobacter coli from human and animal hosts were analyzed by multilocus enzyme electrophoresis. ten genetic loci were characterized by the electrophoretic mobilities of their products, and all were found to be polymorphic. the nine loci considered for genetic analysis had an average allele number of 6.9 and a mean genetic diversity of 0.634. the strains were grouped into 64 electrophoretic types (ets) forming two principal clusters; the first ...19892707852
comparison of different vaccines and induced immune response against campylobacter jejuni colonization in the infant mouse.the degree of protection conferred by vaccinated dams on infant mice against colonization by campylobacter jejuni depended on the bacterial strain, preparation, and route of administration of the vaccine. in some instances of homologous protection, serum bactericidal titres correlated well with protection. however, boiled c. jejuni vaccine, which was non-protective, also elicited a strong bactericidal antibody response. conversely, bactericidal activity could not be demonstrated against strains ...19892703020
serotyping studies of campylobacter from naturally colonized chickens.campylobacter jejuni/coli strains from 164 chickens were serotyped by the methods previously described by penner et al. and lior et al. the chickens were sampled during breeding from hatching to the age of 42 weeks. the birds were housed, in two separate groups, under different environmental conditions, (for comparison of the effect of hygienic precautions on the transmission of the bacteria during breeding). in the group where the hygienic conditions could be controlled to a greater extent, the ...19892703018
[a case report of campylobacter fetus subspecies fetus bacteremia].an unusual case of campylobacter fetus subspecies fetus bacteremia was presented. a twenty four year old male was admitted to our hospital due to abdominal pain, general malaise, diarrhea, high fever, and hemoptysis. he was alcoholic and fond of eating raw liver. he had a history of partial gastrectomy and disturbance of pancreatic function. he showed pulmonary empyema, pleuritis, thrombophlebitis of lower legs, jaundice, hepatomegaly, diarrhea, pneumothorax, and low t3 low t4 syndrome. c. fetus ...19892695582
campylobacter fetus subsp. jejuni in poultry reared under different management systems in nigeria.cloacal swabs from 487 live birds in 36 flocks and 70 poultry carcasses were cultured for campylobacter fetus subsp. jejuni. it was isolated from 12.3% of the birds in 19 flocks. chickens, turkeys, and guinea fowl differed from one another in isolation rates of the organism. management system affected its occurrence, and only 7.1% of eviscerated carcasses yielded it. it was concluded that bird species, management system, and immersing slaughtered poultry in boiling water before dressing affect r ...19892695052
applications of a new method for devising bacteriological serotyping schemes.greatly simplified serotyping schemes were devised for two organisms, escherichia coli and campylobacter jejuni. the mathematical approach developed to improve the e. coli serotyping routine at the national health institute in new zealand was used on three previously published data sets. it had been considered possible that the mathematical method used in the first study was specific to that data set and therefore not applicable elsewhere. this paper verifies that the mathematical method works o ...19892685036
campylobacter fetus subspecies fetus infection of an abdominal aneurysm.we report a case of aneurysm of the abdominal aorta with spontaneous rupture in a 64-year-old man with emphysema and chronic alcoholism. campylobacter fetus subspecies fetus was repeatedly recovered from the blood and from specimens of the excised aneurysm. the patient died in spite of early diagnosis, intravenous erythromycin and surgical intervention. the bacteriology and epidemiology of campylobacteriosis in man is briefly discussed and eight other published cases of aneurysm, infected with t ...19892678860
[neurologic involvement in campylobacter infections. 5 cases].campylobacter are a newly recognized class of human infectious agents. campylobacter fetus subspecies fetus is a cause of sepsis in immunocompromized hosts. secondary neurological determinations, meningitis and meningoencephalitis appear to be rare. we report 2 cases, and 8 previously reported cases are reviewed. campylobacter jejuni appears to be a common bacterial pathogen causing a syndrome of enteritis. since 1982 it has been associated with guillain-barré syndrome (gbs). in one serological ...19892664975
[infectious diarrhea diseases].gastroenteritis may be due to many agents, infectious or non infections. most cases of infections diarrhea are selflimited--e.g. food born intoxications with 1 to 2 days duration or infections with enteropathogenic e. coli, salmonella spp., vibrio parahaemolyticus or rota virus. even the majority of shigellosis, salmonella-gastroenteritis and infections with campylobacter fetus cease without treatment within 4 days. fever and diarrhea persisting for more than 4 days justify the use of antibiotic ...19892662618
response of the chick embryo to live and heat-killed campylobacter jejuni injected into the yolk sac.graded doses of live and heat-killed cells of campylobacter jejuni were injected into the yolk-sac of 5-day-old chick embryos, and the 50% lethal dose (ld50) was determined 7 days later. a strain dependent virulence was seen. in the diluted series of cultures the ld50 values for live campylobacter ranged from 10(6) c.f.u. beyond the last dilution showing growth, that is to less than one organism per embryo. when the 22 strains were tested as heat-killed cells, the chick embryo ld50 values retain ...19892606163
bacterial infertility in camels (camelus dromedarius): isolation of campylobacter fetus.this study constitutes the first reported isolation of c. fetus subsp. venerealis from cervical swabs in breeding camels. 9 of 30 camels which were barren for a long period were found to be positive for c. fetus. the methods of isolation are described. additionally, cervical flora of 30 camels were examined. 9 different bacterial species were isolated of which staphylococcus aureus was most frequently found. all samples were negative for trichomonas sp. and streptococcus zooepidemicus.19892598805
human antibody response to campylobacter jejuni flagellin protein and a synthetic n-terminal flagellin peptide.we measured isotype-specific human antibodies directed against campylobacter jejuni native flagellin and a synthetic peptide derived from the n-terminal amino acid sequence of the protein by using a microdilution enzyme-linked immunosorbent assay (elisa). serum samples from patients with gastrointestinal infection caused by c. jejuni (n = 20) and control samples (number from normal subjects = 20; number from patients with diarrhea other than campylobacter = 20) were tested in this assay. serum s ...19892584372
antigenic heterogeneity of lipopolysaccharides from campylobacter jejuni and campylobacter fetus.the lipopolysaccharide (lps) structure of campylobacter spp. can be visualized with polyacrylamide gel electrophoresis by examining proteinase k-treated whole cell lysates. polyacrylamide gel electrophoresis lps profiles of c. jejuni strains are rough type with low concentrations of low-molecular-weight polysaccharide side chains, serum-resistant c. fetus strains have smooth-type lps, and serum-sensitive c. fetus strains have rough-type lps. we electroblotted the proteinase k-treated whole cell ...19852580793
western blot analysis of the human antibody response to campylobacter jejuni cellular antigens during gastrointestinal infection.western blot analysis was used to identify antigenic components of campylobacter jejuni whole cells and outer membranes that elicit antibody responses in patients with campylobacter enteritis. acute- and convalescent-phase sera from eight patients were analyzed for antibody activity against their homologous infecting strains and heterologous clinical isolates. whole-cell and sarkosyl-insoluble membrane components were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and tra ...19852578479
antigenic analysis of campylobacter flagellar protein and other proteins.outer membrane proteins of campylobacter jejuni and other campylobacter species were analyzed for their antigenic potentials by immunoblotting. polypeptides were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred electrophoretically, and reacted with rabbit antisera to c. jejuni. each campylobacter species analyzed demonstrated a unique outer membrane protein antigenic profile; interspecies antigen sharing was observed to be compatible with the degree of dna relat ...19852578478
adhesion to and invasion of hep-2 cells by campylobacter spp.twenty-one isolates were tested for their ability to adhere to and invade hep-2 cells in vitro. of the 21 organisms tested, 2 did not invade the hep-2 cells, and 1 of these did not adhere to the epithelial cells. campylobacter jejuni clinical isolates were more invasive than the nonclinical strains that were tested. when hep-2 cells were treated with cytochalasin b, the invasiveness of c. jejuni was reduced, indicating active participation of the host cell in the uptake of these organisms. the n ...19892550368
antigenic and restriction enzyme analysis of isolates of campylobacter fetus subsp venerealis recovered from persistently infected cattle.thirty-two isolates of campylobacter fetus subsp venerealis were obtained from 1 bull and 4 heifers with experimentally induced infection. when whole-cell antigens of isolates were cross titrated with antisera to the infecting strain, isolates from 3 heifers had limited antigenic variation, whereas whole-cell antigens of isolates from 2 cattle (the bull and a heifer) differed serologically from those of the infecting strain. changes were detected specifically in 6 heat-labile antigens. of the 6 ...19892548417
abdominal aortic aneurysm infected with campylobacter fetus subspecies fetus.we report a survivor of campylobacter fetus septicemia from an infected abdominal aortic aneurysm who was successfully treated with an anatomic graft reconstruction and antibiotics. according to a survey of the english-language medical literature this was the fourth such patient successfully treated. c. fetus sepsis associated with an abdominal aortic aneurysm was first reported in 1971. the first patient to survive reconstruction of an aortic tube graft aneurysm infected with c. fetus was repor ...19892527318
rapid method for the detection of dnase of campylobacters.a rapid agar diffusion method for the detection of dnase production of campylobacter jejuni, c. coli, and c. pylori was developed. a strong pink zone indicating dna hydrolysis was seen around the wells after 20 to 24 h of aerobic incubation at 37 degrees c. pretreatment of cells with polymyxin b, which releases the cell-associated dnase, both shortened the time needed to read positive results to 8 h and increased the zone size.19892506224
[the most frequent infectious causes of abortion in sheep in north bavaria with special reference to chlamydia and salmonella infections].between 1980 and 1987 1153 ovine fetuses and placentas were examined after abortion. in 68.5% of the cases a cause of abortion could be diagnosed as follows: 43.5% chlamydia psittaci var. ovis, 10.7% salmonella abortus ovis, 3.7% coxiella burnetii, 3.3% listeria monocytogenes and 0.4% campylobacter fetus intestinalis. hemolyzing escherichia coli and streptococci, yersinia pseudotuberculosis, salmonella typhimurium, salmonella enteritidis, corynebacterium pyogenes and other facultative pathogens ...19892503908
pancreatitis associated with campylobacter jejuni infection: diagnosis by ultrasonography. 19892499366
[sepsis caused by campylobacter fetus spp. fetus]. 19892491527
prospective study of campylobacter jejuni infection in chilean infants evaluated by culture and serology.a prospective study of campylobacter jejuni infection was performed during a 6-month period in a cohort of 198 chilean infants. surveillance was based on biweekly home visits by a team of trained nurses. c. jejuni colonization was studied by culturing stool samples from diarrheal episodes and paired asymptomatic controls and by performing an enzyme-linked immunosorbent assay developed to measure c. jejuni outer membrane protein polyvalent antibodies in a representative group of infants. c. jejun ...19892473090
flagellin expression in campylobacter jejuni is regulated at the transcriptional level.campylobacter jejuni 81116 is able to switch flagellum formation on and off. to study the expression of flagellin, the main component of flagella, an expression library of c. jejuni dna was constructed in lambda gt11. screening of this library with a flagellin-specific antiserum resulted in a clone producing a beta-galactosidase-flagellin fusion protein; it contained a dna insert of 850 base pairs and coded for about 15 kilodaltons of the flagellin, corresponding to 410 base pairs of the flagell ...19892466792
experimental production of proliferative ileitis in syrian hamsters (mesocricetus auratus) by using an ileal homogenate free of campylobacter jejuni.the role of campylobacter jejuni in the pathogenesis of proliferative ileitis of syrian hamsters (mesocricetus auratus) has been uncertain. c. jejuni has been implicated as the etiologic agent on the basis of the campylobacter-type morphology of the intracellular organism and the repeated microbiologic isolation of c. jejuni from hamsters with proliferative ileitis. the inability to reproduce the disease with pure culture inocula, coupled with immunohistochemical studies, however, has suggested ...19892462537
location of epitopes on campylobacter jejuni flagella.flagella were isolated from strains of campylobacter jejuni belonging to different heat-labile serogroups and from a strain of campylobacter fetus, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the flagellin molecular weights (mr) were approximately 62,000. the flagellins were cleaved by hydrolysis with cyanogen bromide, and sodium dodecyl sulfate-urea peptide gel electrophoresis showed that the c. jejuni flagellins were structurally similar, and differed from c. fetu ...19862430943
isolation and characterization of a common antigen in campylobacter jejuni and campylobacter coli.flagellin protein was isolated and purified from two serotype reference strains of campylobacter jejuni, pen 1 and pen 3. each preparation was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to consist of a diffuse band with a molecular mass of approximately 62 kilodaltons. antisera were prepared against flagellin from pen 1, and specific antibody was isolated by affinity chromatography with flagellin protein covalently bound to cyanogen bromide-activated sepharose. the high-a ...19862424932
monoclonal antibodies directed against the flagella of campylobacter jejuni: production, characterization and lack of effect on the colonization of infant mice.eight monoclonal antibodies have been derived from balb/c mice hyperimmunized with the purified flagella from campylobacter jejuni strain 81116. these monoclonal antibodies are directed against flagella as demonstrated by reaction in elisa against flagellate and aflagellate antigens, radio-immunoprecipitation and electro-immunoblotting techniques. some of the antibodies react with a 60k minor protein as well as the 62k flagella protein. this protein may be related to an antigen expressed on the ...19862422256
basis for serological heterogeneity of thermostable antigens of campylobacter jejuni.lipopolysaccharides (lps) were extracted from eight strains of campylobacter jejuni and purified by enzyme treatment to remove traces of rna, dna, and protein. this material was used to sensitize sheep erythrocytes for the passive hemagglutination assay that is presently used to serotype c. jejuni. the results confirmed that the thermostable antigen typing scheme is based on lps (o) antigens. the lps after sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining was found to ...19852412962
interaction of campylobacter jejuni and campylobacter coli with lectins and blood group antibodies.lectins and blood group antibodies were used to probe the surface structures of campylobacter jejuni and campylobacter coli. of the 29 strains tested, there were distinct reaction patterns. the lectin-reactive and blood group antibody-reactive sites on the bacterial surface were distinguishable from the heat-stable (lipopolysaccharide) antigenic determinants. the interactions were strain specific. the reactive sites were stable with respect to culture media and passage and may be useful as addit ...19852410445
efficacy of a vaccine to prevent chlamydia- or campylobacter-induced abortions in a sheep flock, chlamydia psittaci, campylobacter fetus, ca jejuni, and salmonella dublin caused abortions. a vaccine that contained c psittaci type i from 2 sources: a cow with pneumonia and an aborted ovine fetus, ca fetus, ca jejuni, and 4 strains of k99 escherichia coli was given to 240 ewes before they were bred. all fetuses, placentas, and lambs, that died within 36 hours of birth were examined for infectious agents. of 55 abortions, 30 (55%) were caused by chlamydia or campylobacter spp ...19902407704
natural transformation in campylobacter species.growing cells of campylobacter coli and c. jejuni were naturally transformed by naked dna without the requirement for any special treatment. transformation frequencies for homologous chromosomal dna were approximately 10(-3) transformants per recipient cell in c. coli and 10(-4) in c. jejuni. maximum competence was found in the early log phase of growth. campylobacters preferentially took up their own dna in comparison with escherichia coli chromosomal dna, which was taken up very poorly. three ...19902404960
the application of a transport and enrichment medium to the diagnosis of campylobacter fetus infections in bulls.the use of a transport and enrichment medium (tem) in the diagnosis of campylobacter fetus infections in bulls is described. the medium significantly improved the diagnosis rate in samples which, because of the length of time between collection and receipt at the laboratory, were unsuitable for processing by direct culture. the tem was able to support the viability, and subsequent multiplication, of c. fetus in some samples for up to 7 days before the tem was incubated. the submission of paired ...19902397373
the development of a transport and enrichment medium for campylobacter fetus.a transport and enrichment medium was developed for campylobacter fetus. from inocula of between 10 and 35 organisms the medium was able to support the multiplication of 19 of 21 strains of c. fetus if the medium was incubated immediately after inoculation; when incubation was delayed for 3 days after inoculation, only seven of the 21 strains multiplied. from inocula of 100-350 organisms all 21 strains multiplied following immediate incubation, and 20 of 21 when incubation was delayed for 3 days ...19902397372
surface array protein of campylobacter fetus. cloning and gene structure.the high molecular mass (97-149 kda) surface array proteins (sap) of campylobacter fetus are critical to virulence. we created a bank of 160,000 random 1.0-6.5-kilobase (kb) chromosomal dna fragments of c. fetus strain 84-32 (23d) using lambda gt11. screening this bank in escherichia coli y1090 with antibody raised against purified sap permitted isolation and purification of a clone with a 4.0-kb insert. subcloning this insert in the e. coli vector, puc9, permitted expression of a protein of app ...19902387868
surface array proteins of campylobacter fetus block lectin-mediated binding to type a lipopolysaccharide.campylobacter fetus strains with type a lipopolysaccharide (lps) and a surface array protein layer (s+) have been found to be pathogenic in humans and animals. spontaneous laboratory mutants that lack surface array proteins (s-) are sensitive to the bactericidal activity of normal human serum. the ability of lectins to determine the presence of the s-layer and differentiate lps type was assessed. we screened 14 lectins and found 3 (wheat germ agglutinin, bandeiraea simplicifolia ii, and helix po ...19902387622
in vitro susceptibility of campylobacter jejuni to rokitamycin.the susceptibilities of 100 clinical isolates of campylobacter jejuni to rokitamycin, a new macrolide, and eight other oral antibiotics were tested by using a broth microdilution method. rokitamycin demonstrated antibacterial activity that was higher than those of fosfomycin and the tetracyclines and comparable to those of erythromycin and clindamycin, whereas it was slightly less active than the quinolones.19902386373
use of an alkaline phosphatase-labeled synthetic oligonucleotide probe for detection of campylobacter jejuni and campylobacter coli.a commercially available synthetic nucleic acid probe (snap) conjugated to alkaline phosphatase was compared with standard culture techniques for detecting campylobacter species. the snap was able to detect either 5 ng of c. jejuni dna or 10(5) cfu of bacteria. the snap could also detect dna extracted from 10(5) cfu in mock-infected stool samples. the snap detected c. jejuni and c. coli but showed no reactivity with c. laridis, c. fetus subsp. fetus, c. fetus subsp. venerealis, c. fennelliae, "c ...19902380381
purification, characterization, and localization of a protein antigen shared by thermophilic campylobacters.a protein antigen with an apparent molecular weight (mr) of 31,000 was isolated from 0.2 m glycine hydrochloride (ph 2.2) extracts of a typical human fecal isolate, campylobacter jejuni vc74. the protein was purified to homogeneity on a preparative scale by immunoaffinity chromatography followed by molecular sieving with a superose 12 column. isoelectric focusing under nondenaturing conditions indicated a pi of 9.3, and amino acid composition analysis showed that the protein was unusually rich i ...19902380360
very long term diarrhoea due to campylobacter jejuni. 19902371198
polynucleotide sequence relationships among flagellin genes of campylobacter jejuni and campylobacter coli.dna probes that encode a complete flagellin gene and various internal regions of the campylobacter coli vc167 flagellin genes were hybridized to 30 strains of c. coli or c. jejuni from 20 different lior serogroups. the results indicated a high overall degree of homology among all of the strains examined. although the most variable regions occurred within the middle of the gene, significant dna homology was observed among many serogroups in this region of the molecule.19902370114
partial purification and characterization of the enterotoxin produced by campylobacter jejuni.campylobacter jejuni enterotoxin was partially purified from culture supernatant. the purified fraction after gel filtration indicated three bands at 68, 54, and 43 kilodaltons on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). this fraction enhanced the adenylate cyclase activity of hela cell membranes by 1.5-fold over that of the control. the study with anti-cholera toxin immunoglobulin g (igg) and ganglioside affinity column chromatographies revealed that the eluent from ...19902370099
infection of adult syrian hamsters with flagellar variants of campylobacter jejuni.two variants of campylobacter jejuni in1 differing in the expression of flagella, in1 (fla+ mot+, wild type) and in1-nm (fla- mot-), were tested for their ability to establish infection in adult hamsters. animals were challenged intracecally with 2 x 10(9) to 5 x 10(10) cfu and monitored for evidence of infection. none of the challenged animals developed illness. there was a significant difference, however, in the ability of in1 to infect hamsters (35 of 43) compared with that of in1-nm (1 of 42 ...19902365459
evidence of reinfection with multiple strains of campylobacter jejuni and campylobacter coli in macaca nemestrina housed under hyperendemic conditions.a prospective bacteriologic study of 18 infant pig-tailed macaques (macaca nemestrina) housed in a nursery facility in which campylobacter spp. are endemic was undertaken to determine the epidemiology of infection and reinfection. the isolates of campylobacter jejuni and c. coli cultured from 8 of the 18 infants were characterized by serotyping, dna hybridization, and polyacrylamide gel electrophoresis protein profiles. the chronology of infection was indicative of multiple reinfections with dif ...19902365455
serogroups of campylobacter fetus and campylobacter jejuni isolated in cases of ovine abortion.of 38 aborted ovine fetuses from 23 sheep flocks 29 c. fetus subsp. fetus and 22 c. jejuni were isolated and examined biochemically and serologically for heat-stable antigens. serologic examinations were carried out by passive haemagglutination test. in case of c. fetus subsp. fetus strains alkaline antigen extraction was used. antisera to two serogroups of c. fetus and to penner serotype reference strains 1 to 60 were produced in rabbits. abortion was caused in 18 (78.3%) flocks by c. fetus sub ...19902363324
bismuth subsalicylate in the prevention of colonization of infant mice with campylobacter jejuni.infant mice were used for the evaluation of the efficacy of bismuth subsalicylate (bss) in the prevention of the growth of campylobacter jejuni in the intestine. the mic90 of ten c. jejuni strains was 900 micrograms/ml. of three dosage regimens tested, continuous treatment before and after the bacterial challenge, mimicking the way bss is used in the prevention of traveller's diarrhoea, was the most effective. growth inhibition was dose dependent; the high dose of 2000 micrograms per day was mor ...19902347383
a domestic ferret model of immunity to campylobacter jejuni-induced enteric disease.oral or intravenous inoculation of previously unexposed juvenile and adult ferrets with campylobacter jejuni uniformly resulted in intestinal colonization lasting 2 to 12 days. disease varied from mild to moderate diarrhea, which resolved in 2 to 3 days. orally infected animals developed agglutinin titers of 8 to 256 within 3 weeks, while those infected intravenously developed titers of 256 to 2,048. ferrets which had recovered from campylobacteriosis all developed high titers of agglutinating a ...19902341180
[a case of right-sided infective endocarditis in a drug addict].a 49-year-old man was admitted to mitsui memorial hospital because of fever of unknown origin. since one year ago, he had often used intravenous narcotic drugs. ten months before his admission, he had his first experience of fever and a chill. four months later, he was admitted to a hospital under the diagnosis of pneumonia and he was treated successfully. after his discharge, he began to use drugs again, which resulted in the repetition of fever and chills during four months prior to his admiss ...19902330463
factors influencing the duration of acute diarrheal disease in infancy.the majority of episodes of acute infectious diarrhea in infancy are of relatively short duration. prolongation of the diarrhea presents problems in management, deterioration of the infant's nutritional status, and an increased mortality. as part of a prospective study of the etiology of acute infectious diarrhea, some factors associated with the probability (more or less) of having self-limiting disease have been identified. the well-nourished infant over 6 months of age is more likely to have ...19902324877
campylobacter fetus infections in two patients with aids. 19902319151
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