PMID(sorted descending)
serological types of vibrio fetus var. intestinalis causing ovine vibriosis in southern australia. 19744819466
human infection with vibrio fetus.vibrio fetus, a slowly growing, microaerophilic, gram-negative rod which causes infectious abortion in cattle, occasionally induces disease in man. since persons receiving immunosuppressive agents are among those most susceptible to this infection, recognition of the disease may become increasingly important as transplantation operations increase. vibrio infections may produce a spectrum of acute and chronic illnesses or may be asymptomatic. vibriosis may develop at any age but it seems most dev ...19744816399
[campylobacter fetus and infectious arthritis]. 19734767883
persistent genital tract infection with vibrio fetus intestinalis associated with serotypic alteration of the infecting strain. 19734748723
[infection caused by vibrio fetus]. 19734739310
vibrio fetus infection in bulls: curative and preventive vaccination. 19734727795
septicemia and gastroenteritis due to vibrio fetus. 19734698759
the effects of erythritol, fsh and female gonadal hormones on the growth in vitro of vibrio fetus var. venerealis. 19734692342
vibrio fetus infection in man: a serological test.antigen preparations derived from a typical human strain of vibrio fetus were employed in four tests. of these, the indirect bacterial hemagglutination test proved most sensitive. by this test, antibodies titering 320 to 3,200 were found in five of eight patients with confirmed infections. two patients without antibodies were on antimetabolites. antigenic relationship with other compounds, and in particular with brucella organisms, was not observed. no sero-reactors were found among 184 apparent ...19724673792
the origin, class and specificity of immunoglobulins in bovine cervico-vaginal mucus: variation with parenteral immunization and local infection with vibrio fetus. 19724648121
immunoglobulin classes and biological functions of campylobacter (vibrio) fetus antibodies in serum and cervicovaginal mucus.serum and cervicovaginal mucus (cvm) antibodies from heifers after genital infection or systemic immunization with campylobacter (vibrio) fetus were classified according to their immunoglobulin class, antigenic specificities, and biological functions. only immunoglobulin (ig) a antibodies, specific both for o and superficial, heat-labile, whole-cell (w) antigens, were detected in cvm of convalescent animals. after systemic immunization, antibodies in serum were directed principally to w antigens ...19744609902
bactericidal activity of blood of rabbits vaccinated with homologous antigens of campylobacter fetus (vibrio fetus).rabbits were vaccinated with the following campylobacter fetus var. venerealis (vibrio fetus) antigens: whole-cell (wc), autoclaved (a), boiled (b), and purified postgrowth broth (pgb). bactericidal activity of freshly drawn heparinized blood against the organism was determined after each vaccination. in all cases bactericidal activity of the blood of vaccinated rabbits was higher than for nonvaccinated rabbits. the in vitro bactericidal activity of the blood was determined in two separate exper ...19744609901
tube culture method for viable counts of campylobacter fetus (vibrio fetus).a method was developed for making viable counts of campylobacter fetus (vibrio fetus) in tube cultures using a medium containing alkaline hematin and incubation in a carbon dioxide incubator.19744607386
studies on the growth conditions of campylobacter fetus. 3. collaborative function of some surfactants in a yeast extract medium for selective cultivation. 19744605173
studies on the growth conditions of campylobacter fetus. ii. the manifestation of nutritional requirements by conditional static cultivation. 19744605172
studies on the growth conditions of campylobacter fetus. 19744605171
campylobacter fetus subspecies jejuni (vibrio fetus) from commercially processed poultry.three isolates of the human and animal pathogen campylobacter fetus ss. jejuni (vibrio fetus) were obtained from 165 poultry meat samples purchased from local retail stores.19744598233
the effect of osmotic variation upon the growth of vibrio fetus. 19744596990
long term storage of vibrio fetus cultures by means of freeze-drying. 19724570341
a method for maintaining the viability of vibrio fetus var. venerealis in samples of preputial secretions collected from carrier bulls. 19724565641
natural and immune antibodies for vibrio fetus in serum and secretions of cattle.naturally occurring antibodies for vibrio fetus with whole-cell and o antigen specificities were detected in sera of mature cattle by means of the agglutination and indirect immunofluorescence reactions. o antibodies occurred in each of 21 sera examined, and whole-cell antibodies, in lower concentrations, occurred in 15 of the 21 sera. natural antibodies were detected in the immunoglobulin g, m, and a (igg, igm, iga) classes. parenteral immunization with whole cells in freund's complete adjuvant ...19724564880
infection patterns in heifers following cervicovaginal or intrauterine instillation of campylobacter (vibrio) fetus venerealis. 19744473318
immunisation against bovine vibriosis. vaccination of bulls against infection with campylobacter fetus subsp. venerealis. 19744441328
the pathogenicity of bovine faecal isolates of vibrio fetus var intestinalis serotype v in pregnant ewes. 19744416400
letter: isolation of campylobacter fetus subsp. venerealis and campylobacter fetus subsp. intermedius from the preputial secretions of bulls. 19744414274
[bacteriological diagnosis of the vibrio fetus infection in bulls]. 19744408642
[2 new cases of septicemia due to campylobacter fetus]. 19734353109
hemorrhagic-necrotic enteritis in a baboon (papio cynocephalus) due to vibrio fetus. 19724343333
chromatographic studies of the lipid components of vibrio fetus. 19714330330
vibriosis: demonstration of vibrio fetus and vibrio bubulus organisms in preputial fluid by immunofluorescence and cultural techniques.fluorescent conjugates were prepared from the sera of calves immunized with four vibrio fetus strains and one vibrio bubulus strain. the fluorescent antibody technique (fat) was then used to detect vibrio organisms in preputial fluid collected from 67 bulls belonging to a canadian artificial insemination (ai) unit. the v. fetus conjugates reacted with both v. fetus var venerealis and v. fetus var intestinalis. v. fetus was found in 20 animals (29.9%), 13 of which also harboured v. bubulus. in tw ...19744277756
bovine vibriosis: the distribution and specificity of antibodies induced by vaccination and infection and the immunofluorescent localization of the organism in infected heifers.two groups of three holstein heifers were immunized respectively with vibrio fetus venerealis and vibrio fetus intestinalis incorporated in freund's complete adjuvant. both serum and vaginal mucus agglutination titers increased following immunization. vaginal mucus samples were more frequently positive when the homologous cells were used as antigen in the agglutination test. ten non-immunized heifers were inoculated with another strain of v. fetus venerealis and slaughtered at periods of 30 to 4 ...19714260944
murein (peptidoglycan) structure of vibrio fetus. comparison of a venereal and an intestinal strain. 19714256272
a study of complement-fixation and serum agglutination tests on vibrio fetus infected and immunized cattle.complement-fixation (cf) and tube agglutination (ta) tests for demonstration of vibrio fetus antibodies were conducted on the sera of three groups of ten heifers. one group was vaccinated subcutaneously with a commercial v. fetus var venerealis bacterin and challenged intra-utero, at the external os cervicus one month later; the second was infected only and the third vaccinated only. the vaccinated cattle developed high cf serum titers, but no such increase was observed in animals infected only. ...19714254893
detection of antibodies to campylobacter fetus (vibrio fetus) in the preputial secretions of bulls with vibriosis. 19744211911
lytic activity of vibrio phages on strains of vibrio fetus isolated from man and animals.five phages isolated from lysogenic strains of vibrio fetus var. venerealis and two from v. fetus var. intestinalis were tested for lytic activity on 95 v. fetus strains from various animal and human hosts. in addition, virion and plaque morphology of the seven phages were compared. electron micrographs showed that all were the kite-tailed variety with minor variations in head and tail dimensions. plaques of v45 and v2 were small, clear and irregular; those of v3, v8, and v19 were large, clear a ...19734201644
fluorescent antibody studies of vibrio fetus: staining characteristics in semen, preputial exudate, and pure culture. 19684170356
further studies on use of polymyxin b sulfate with dihydrostreptomycin and penicillin for control of vibrio fetus in a frozen semen process. 19664166191
isolation and characterization of a bacteriophage for vibrio fetus.bacteriophages were isolated from 22 of 38 strains of vibrio fetus by an enrichment process, utilizing the donor and host strains growing together in fluid thioglycollate medium. one phage, v-45, isolated by the conventional lawn-spot method, was characterized by stability in broth, growth kinetics, and morphology. it was sensitive to rapid thermal inactivation, chloroform, and ph values above 6.5. calcium was required for phage replication and stability in broth. magnesium provided the best pro ...19704097234
experimentally induced vibrio fetus var. intestinalis infection in pregnant cows. 19704097065
[bacteremia caused by campylobacter fetus ssp. fetus]. 19854079571
[clinical studies on the effectiveness of sm-4300, a new non-modified gammaglobulin preparation suitable for intravenous use, in refractory infections].eighteen cases of various infections, mostly having severe underlying diseases and showing no or insufficient responses to antibacterial chemotherapy, were treated with additional intravenous drip infusion of sm-4300 (2.5 or 5 g, once a day, for 1-3 days). eight of the cases were excluded from the assessment of the effectiveness, as they died too early, or as their fever was supposed to have been "tumor fever" retrospectively. sm-4300 was found to be effective in 2 out of 5 septicemia cases, in ...19854079018
inhibition of campylobacter coli and campylobacter jejuni by antibiotics used in selective growth media.the ability of campylobacter coli and campylobacter jejuni to grow in the presence of antibiotics used in selective growth media was compared. mic data for c. coli indicated that some strains were more susceptible to the antibiotics than were the c. jejuni strains tested. a reduction of greater than 1 log cycle in the numbers of cells growing on plates containing antibiotics was considered to be a marked level of inhibition. only one of nine of the antibiotic combinations studied did not markedl ...19854077962
comparison of gauze swabs and membrane filters for isolation of campylobacter spp. from surface water.the epidemiology of campylobacter jejuni indicates that waterborne transmission is important; the organism has been isolated from seawater, fresh water, and estuarine sites. membrane filtration, with and without use of an enrichment broth, has been the most common method for isolating c. jejuni from water. we evaluated two methods for isolating c. jejuni from water: membrane filtration and gauze filtration. the membrane filters evaluated included 0.22- and 0.45-micron-pore millipore filters (mil ...19854073894
investigations on the role of flagella in the colonization of infant mice with campylobacter jejuni and attachment of campylobacter jejuni to human epithelial cell lines.the biochemical and biological properties of the flagella of campylobacter jejuni have been investigated using two variants selected from a flagellate, motile clinical isolate (strain 81116): a flagellate, non-motile variant (sf-1) and an aflagellate variant (sf-2). phenotypic and biochemical analysis of the strains and amino acid analysis of the isolated flagella suggest that the variants differed from the wild-type strain only in the absence of flagella and/or motility. the aflagellate variant ...19854067286
reversible expression of flagella in campylobacter jejuni.campylobacter jejuni 81116 and a3249 undergo a bidirectional transition between flagellated (fla+) and aflagellated (fla-) phenotypes. when measured in culture medium, the fla+----fla- transition occurred at a rate of 3.1 x 10(-3) to 5.9 x 10(-3) per cell per generation, and the fla- to fla+ transition occurred at a rate of 4.0 x 10(-7) to 8.0 x 10(-7) per cell per generation. however, passage through a rabbit intestine markedly favored the fla+ phenotype.19854066041
comparison of methods for isolating campylobacter jejuni from raw milk.the method of doyle and roman (appl. environ. microbiol. 43:1343-1353, 1982) was compared with that of lovett et al. (appl. environ. microbiol. 46:459-462, 1983) for the ability to recover campylobacter jejuni strains inoculated into raw milk at a concentration of less than 1 cell per g. the method of lovett et al. gave significantly greater recovery proportions.19854051491
occurrence of plasmids and antibiotic resistance among campylobacter jejuni and campylobacter coli isolated from healthy and diarrheic animals.serologically defined strains of campylobacter jejuni and campylobacter coli from healthy and diarrheic animals were examined for the occurrence of plasmid dna in association with the antibiotic susceptibility of the bacterial host and the health status of the animal host. of all campylobacter organisms surveyed, 53% (116 of 200) contained plasmid dna. a plasmid occurrence rate of 73.8% was obtained for c. coli from healthy pigs, contrasted by lower plasmid occurrence rates for c. coli from diar ...19854044793
campylobacter fetus peritonitis followed by septicaemia in a patient on continuous ambulatory peritoneal dialysis.a 62-year-old man being treated by continuous ambulatory peritoneal dialysis (capd) developed peritonitis due to campylobacter fetus subspecies fetus (intestinalis), an organism seldom isolated in such circumstances. after appropriate and apparently effective antibiotic therapy, the patient relapsed 6 weeks later with septicaemia. blood cultures yielded a similar organism, thereby suggesting a clinically silent metastatic infection during the episode of peritonitis, probably at an old arterioven ...19854031516
biotyping schemes for campylobacter jejuni. 19854031047
campylobacter colitis: histological immunohistochemical and ultrastructural findings.the colonic biopsy specimens of 22 patients with colitis and positive stool cultures for campylobacter jejuni were studied in order to obtain histological and immunohistochemical criteria to differentiate campylobacter colitis from chronic inflammatory bowel disease. in addition we tried to identify campylobacter inclusions by means of immunohistochemistry and electron microscopy as evidence for invasion of the colonic mucosa. the results show that the majority of patients with campylobacter col ...19854029720
campylobacter and endovascular lesions.because of the gaps in our knowledge of the epidemiology and pathogenesis of campylobacter infections, particularly the propensity of campylobacter fetus ssp fetus to infect vascular endothelium, the authors describe the case of a 56-year-old woman with c. fetus ssp fetus infection of an aortic aneurysm. she recovered after a one-stage surgical repair and antibiotic therapy with erythromycin. the organism was cultured from samples of the stool and tissue obtained at operation. it was identified ...19854027795
enrichment medium for isolation of campylobacter jejuni-campylobacter coli.a broth enrichment medium for the improvement of isolation of campylobacter jejuni-campylobacter coli from stool samples and other specimens is presented. of 1,228 samples examined in parallel, positive results were obtained from 81 by direct inoculation of selective media and from 112 after enrichment. thus, an increase of 27.7% in the isolation rate was obtained by using the enrichment medium. the same medium without antibiotics allows the preservation of isolates of c. jejuni-c. coli for at l ...19854026287
biotypes and serotypes of thermophilic campylobacters isolated from cattle, sheep and pig offal and other red this study we examined 730 faecal samples of offal (mainly liver), mince-meat and sausage meat collected from abattoirs and retail butchers' shops for campylobacters. campylobacter jejuni or c. coli were isolated from 30.6, 10.5 and 6% of sheep, cattle and pig offal samples respectively. specimens collected from abattoirs were, in general, more often contaminated than material obtained from retail butchers' shops. only 1.4% of minced meats and sausage meats contained campylobacters. most isol ...19854020105
development of a bacteriophage typing system for campylobacter jejuni and campylobacter coli.a bacteriophage typing system for campylobacter jejuni and campylobacter coli was developed with phages isolated from poultry feces. data for phage selection were generated from a set of isolates of c. jejuni and c. coli from humans in illinois. selection of 14 phages from the 47 phages available was assisted by determination of the sneath-jaccard similarity coefficients and subsequent unweighted pair-group arithmetic averaging cluster analysis. the typing set was reproducible and stable in the ...19854019737
serotyping of campylobacter jejuni, campylobacter coli, and campylobacter laridis from domestic and wild using 50 unabsorbed antisera, we were able to serotype 272 (65.7%) of 414 thermotolerant campylobacters from wild and domestic animals, on the basis of heat-stable antigens identified by means of passive hemagglutination. forty-two serotypes were recognized. the pattern of serotypes detected in the various animal species was compared to human clinical isolates by using the czekanowski index (proportional similarity index). the highest degree of similarity to the clinical isolates was observed ...19854015088
laboratory infection of chicken eggs with campylobacter jejuni by using temperature or pressure differentials.fertile chicken eggs were infected in our laboratory with campylobacter jejuni suspensions by using temperature or pressure differential methods of inoculation. after 2 days of incubation, over 90% of the eggs carried c. jejuni when iron was present in the inoculum. this percentage declined rapidly until by day 8, less than 10% of the eggs were detectably infected. however, up to 11% of hatched, healthy chicks carried c. jejuni in their intestinal tracts. the isolated organisms were of the same ...19854015086
[ultrastructural study of campylobacter fetus ssp. jejuni]. 19854012096
infected aortic aneurysms.salmonella organisms have been responsible for half of the reported cases of infected aortic aneurysms, although other bacteria have been incriminated. this is the second reported case in which campylobacter fetus subspecies fetus apparently infected an inflammatory aortic aneurysm. in this 70-year-old man the organism was isolated and treated prior to resection of the aneurysm and inline grafting. specific antibiotic therapy was continued for 8 weeks postoperatively. examination and ct scans ob ...19854009843
biochemical and genetic characteristics of atypical campylobacter fetus subsp. fetus strains isolated from humans in the united states.during a 2-year period, 14 biochemically atypical campylobacter fetus subsp. fetus-like strains were received by the campylobacter reference laboratory at the centers for disease control. sources of the isolates were blood, nine strains; stools, two strains; amniotic fluid, one strain; and abscesses, two strains. atypical phenotypic characteristics exhibited by one or more strains were growth at 42 degrees c, 10 strains; no h2s by lead acetate paper, 3 strains; resistance to a 30-micrograms ceph ...19854008624
[digestive complications of campylobacter enteritis].fifty children with acute diarrhoea, in which the only germ found in stools was campylobacter fetus jejuni, are studied. digestive complications occurred are discussed. nine (18%) had lactose malabsorption, associated in two (4%) with saccharose malabsorption and in one (2%) with cow's milk protein intolerance. all complications were temporary, and carbohydrate malabsorption disappeared between one and three months.19854003954
campylobacter jejuni infections on college campuses: a national survey.we surveyed 303 colleges and universities to determine the frequency of isolations of campylobacter jejuni and other enteric bacterial pathogens. campylobacter cultures could be obtained at 74 per cent; 10 per cent of stool cultures yielded campylobacter, which was isolated 10 and 46 times more frequently than salmonella and shigella, respectively. the incidence of diagnosed campylobacter infections was 1.3 per 10,000 enrolled students per month. intercollegiate variation was not explained by di ...19854003634
typing of heat-stable and heat-labile antigens of campylobacter jejuni and campylobacter coli by coagglutination.a coagglutination system has been devised for typing heat-stable and heat-labile antigens of campylobacter jejuni and c. coli. the use of protein a-positive staphylococcus aureus cells carrying campylobacter sp. serotype antibody and the treatment of campylobacter sp. cells with dnase in the antigen suspension permitted rapid and specific coagglutination of rough (autoagglutinable) as well as smooth cultures. cells of s. aureus were sensitized with campylobacter sp. serotype antisera. four to fi ...19853998098
[isolation of campylobacter spp. from calves in bulgaria].studied were a total of 58 samples, containing intestinal content of calves that had died of enteritis as well as fecal samples and samples from clinically normal calves. campylobacter spp. were isolated in 8.8 per cent of the cases. the isolated 5 strains was determined as campylobacter fetus subsp. intestinalis. this is the first communication on the isolation of campylobacter spp. in this country.19853992922
evidence of udder excretion of campylobacter jejuni as the cause of milk-borne campylobacter outbreak.we describe a community outbreak of campylobacter enteritis associated with the consumption of untreated milk, apparently contaminated by two cows with campylobacter mastitis. the outbreak occurred in two phases. strains of campylobacter jejuni of the penner serogroup complex 4, 13, 16, 50 and preston biotype code 6100 were isolated from patients in both episodes and from the faeces of the cattle, milk filters, bulk milk and retail milk. milk samples from two of 40 milking cows were found to con ...19853989284
phagocytosis of campylobacter jejuni and its intracellular survival in mononuclear vitro phagocytosis and intracellular survival of campylobacter jejuni strain 2964 in mononuclear phagocytes were studied. the following three types of mononuclear phagocytes were used: a j774g8 peritoneal macrophage line derived from balb/c mice, resident balb/c peritoneal macrophages, and human peripheral blood monocytes. when c. jejuni and mononuclear phagocytes were combined at a ratio of 75:1, light microscopy, fluorescent microscopy, and electron microscopy all indicated that c. jejuni c ...19853988342
use of auxotyping for epidemiological studies of campylobacter jejuni and campylobacter coli infections.a chemically defined medium developed for neisseria gonorrhoeae was modified to support the growth of campylobacter jejuni and campylobacter coli. a total of 76 isolates of c. jejuni and 14 isolates of c. coli were tested on this medium, which was designated campylobacter defined medium (cdm), over a 3-month period. although none of the c. coli isolates appeared to require amino acids, 51% of the c. jejuni tested required one and 7% required multiple amino acids for growth. an analysis of isolat ...19853988340
campylobacter fetus subspecies fetus bacteremia.eight patients with campylobacter fetus bacteremia, six of them with serious underlying diseases, were seen in a two-year period. besides fever, which was observed in all cases, the most frequent clinical manifestation was lower extremity phlebitis and cellulitis (four patients). in one of these patients, it had the peculiar aspect of bilateral pretibial cellulitis. one patient had vertebral osteomyelitis, a complication, to our knowledge, not yet described. two patients, both with advanced unde ...19853977489
enzyme-linked immunosorbent assay for antibodies to campylobacter fetus in bovine vaginal enzyme-linked immunosorbent assay (elisa) was used to detect antibodies to campylobacter fetus subspecies venerealis in bovine vaginal mucus. the results of testing 168 samples from experimentally infected, field cases and control cows showed that the elisa was more sensitive than the vaginal mucus agglutination test and also detected antibodies in earlier stages of infection.19853975480
the virulence of clinical and environmental isolates of campylobacter jejuni.the virulence of campylobacter jejuni and c. coli isolated from various water sources was compared with that of clinical strains by in vitro assays of adhesion, invasion and cytotoxicity to hela cells. variation in degree of attachment was observed, but this did not appear to be related to strain source, however, water strains were less invasive and less cytotoxic to hela cells than clinical strains as shown by immunofluorescence and electron microscopy. these differences were particularly evide ...19853973380
isolation of campylobacter fetus from a pet turtle.during the course of a salmonella agona case investigation, campylobacter fetus was isolated from a pet turtle. this is the first reported isolation of c. fetus from a turtle and suggests that turtles, in addition to being reservoirs for salmonella species, may also be reservoirs for c. fetus.19853972996
comparison of basal media for culturing campylobacter jejuni and campylobacter coli.four strains of campylobacter jejuni and four strains of campylobacter coli were used to compare the quantitative growth of campylobacter cells on blood agar base no. 2 (oxoid), brucella agar (bbl microbiology systems and difco laboratories), campylobacter agar base (difco), columbia blood agar base (difco and oxoid), and mueller-hinton agar (difco and oxoid). columbia blood agar base and blood agar base no. 2 were inhibitory to most of the strains tested, as evidenced by reduced (10- to 1,000-f ...19853972990
campylobacter jejuni-specific serum antibodies are elevated in healthy bangladeshi bangladesh and other developing countries, isolation of campylobacter jejuni is common in healthy children, and the illness/infection ratio falls with age. to determine whether specific serum antibodies correlate with this phenomenon, using an enzyme-linked immunosorbent assay, we studied sera from 93 healthy bangladeshi children and 121 healthy u.s. children under 15 years of age. for each age group (less than 1, 2 to 4, and 5 to 14 years) studied, specific serum antibody levels were signifi ...19853972984
susceptibility of campylobacter isolates to the bactericidal activity of human serum.although campylobacter jejuni and related thermophilic organisms are more common human pathogens than are campylobacter fetus, most bloodstream or systemic isolates are c. fetus. to understand the pathophysiology related to this observation, the authors studied susceptibility to the bactericidal activity of normal human serum of campylobacter coli, c. jejuni, and c. fetus isolates from feces and blood. in standardized assays, 10 of 15 c. jejuni and related isolates showed 90% kill (mean, 90.6% + ...19853968449
in vitro antimicrobial susceptibility, plasmid analysis, and serotyping of epidemic-associated campylobacter jejuni.campylobacter jejuni strains from 11 outbreaks were characterized by antimicrobial susceptibility, plasmid profile, and serotyping by the methods of lior et al. and penner and hennessy. all 31 strains were susceptible to erythromycin, clindamycin, chloramphenicol, kanamycin, tobramycin, streptomycin, and gentamicin. a total of 21 strains from nine outbreaks were resistant to one or more of the following antimicrobial agents: tetracycline, metronidazole, ampicillin, or carbenicillin. of the 31 st ...19853968203
lipopolysaccharide characteristics of pathogenic campylobacters.most campylobacter jejuni strains are sensitive and most campylobacter fetus strains are resistant to the bactericidal activity in normal human serum. we purified lipopolysaccharides from campylobacter strains to determine whether their composition and structure relate to serum susceptibility. the lipopolysaccharide of two serum-sensitive strains was best isolated by the galanos procedure, but for two serum-resistant strains a cold-ethanol extraction was optimal. for each lipopolysaccharide prep ...19853967920
campylobacter jejuni septicemia--epidemiology, clinical features and 33 cases of campylobacter jejuni septicemia, the disease was more common at the extremes of age: infants made up a third of the reported cases while 24% of patients were older than 50 years. fever was noted in more than 80% of patients and chills in about a fourth. enteritis was present in 70% of cases, and the gastrointestinal tract was the principal source of septicemia. half of the patients did not have significant underlying disease but were at extremes of age, which may reflect relative ...19863962297
distribution and serotypes of campylobacter jejuni and campylobacter coli in enteric campylobacter strains isolated from children in the central african hundred eighty-five enteric campylobacter strains isolated from diarrheic or healthy children in bangui (central african republic) were studied to determine their species and serotypes. c. coli was identified in 38.9% of all strains and in 43.9% of strains from diarrheic children. by the hemagglutination technique for heat-stable antigens, 73.5% of the strains could be serotyped. of the typeable strains, 75% were distributed among 13 more frequent serotypes. c. coli serotype pen 37,56 was th ...19863958146
evaluation of a blood-free, charcoal-based, selective medium for the isolation of campylobacter organisms from feces.a blood-free, charcoal-based selective medium (csm) consisting of a columbia agar base, activated charcoal (4 g/liter), hematin (0.032 g/liter), sodium pyruvate (0.1 g/liter), cefoperazone (32 mg/liter), vancomycin (20 mg/liter), and cycloheximide (100 mg/liter) supported the growth of campylobacter jejuni and c. coli with colony counts equivalent to those obtained on antibiotic-free horse blood agar. csm was compared to skirrow medium (skm) for the recovery of c. jejuni and c. coli from stools ...19863958143
abortion in sheep caused by a nonclassified, anaerobic, flagellated bacterium.twenty-eight pregnant ewes were inoculated iv with approximately 6 x 10(8) nonclassified, anaerobic, flagellated bacteria (nafb) that had been isolated from an aborted lamb. abortion occurred in 3 of the ewes and 1 ewe gave birth to a weak lamb. the remaining 24 ewes and 3 other ewes inoculated orally with nafb did not develop clinical signs of illness. suppuration and vasculitis were seen in the placentas of the 3 aborted lambs, 1 of which had necropurulent hepatitis indistinguishable from that ...19863954201
epidemic campylobacteriosis associated with a community water may 1983, an estimated 865 cases of epidemic gastrointestinal disease occurred in greenville, florida. surveillance of pharmacy sales of antidiarrheal medicines suggested that the outbreak was confined to greenville and its immediate vicinity. surveys demonstrated that the gastrointestinal illness attack rates inside and outside the city limits were 56 per cent (72/128) and 9 per cent (7/77), respectively (relative risk (rr) = 6.2); consumption of city water was associated with illness (rr = ...19863953920
lectin agglutination for the differentiation of campylobacter fetus serotypes. 19863952971
campylobacter fetus as a cause of acute cholecystitis. 19863947876
morphologic observations of experimental campylobacter jejuni infection in the hamster intestinal tract.the authors have developed a model for the diarrhea and intestinal lesions seen in campylobacter jejuni enterocolitis by colonizing the hamster ileum and cecum with c jejuni. erythematous inflammation of the ileum and cecum and distention of the cecum with fluid were observed at autopsy. the cecal mucosa appeared edematous. epithelial abnormalities observed by light microscopy included focal edema, occasional hyperplasia, diffuse hyperemia, and infiltration of the lamina propria with leukocytes. ...19863942198
campylobacter jejuni infection in colorado: unexplained excess of cases in males.between january 1, 1981, and december 31, 1982, the colorado department of health received reports of 1,185 culture-confirmed cases of campylobacter jejuni infection. incidence rates were highest among infants less than 1 year old and among persons aged 20-29 years. the distribution of cases by sex showed a predominance among males at all ages except 40-59 years, the most marked predominance occurring in infants under 1 year. the higher rates for males were also significant for all ages combined ...19853923544
immunological relationship of the b subunits of campylobacter jejuni and escherichia coli heat-labile enterotoxins.the application of dissociation techniques, involving gel filtration in the presence of guanidine, to a semipurified preparation of campylobacter jejuni heat-labile enterotoxin yielded a material whose functional and immunological properties resemble those of the b subunits of cholera toxin and escherichia coli heat-labile toxin (lt). the c. jejuni toxin b subunit reacted with gm1 ganglioside in an enzyme-linked immunosorbent assay, but lacked the holotoxin's cytotonic activity in the chinese ha ...19853922890
[campylobacter fetus ssp. jejuni, aeromonas hydrophila, helicoidal bacteria and coronavirus in the murine intestine].intestinal contents of 28 laboratory-bred white mice and 6 wild-caught rats were extracted and observed with phase-contrast and transmission electron microscopy; cultures were made in butzler agar and aeromonas, incubated in microaerobiosis, at 37 degrees c for 5 days. in three mice and two rats, helicoidal bacteria were observed, with 8 to 11 periplasmic fibers and terminal branches of 8 to 11 structures, similar to flagella. in one of the rats, coronavirus-like particles were observed. campylo ...19853916393
pathogenic properties of campylobacter jejuni: assay and correlation with clinical manifestations.the pathogenic properties of 20 strains of campylobacter jejuni isolated from persons with clearly defined clinical manifestations were determined. cell-free broth filtrates were examined for (i) enterotoxin production by chinese hamster tissue culture assay and an enzyme-linked immunosorbent assay (elisa) employing gm1 ganglioside and affinity-purified antiserum to escherichia coli heat-labile toxin, (ii) cytotoxin production by vero and hela cell tissue culture lines, and (iii) their ability t ...19853899937
occurrence of campylobacter jejuni and giardia species in muskrat (ondatra zibethica).a total of 189 muskrat fecal samples were surveyed for campylobacter and giardia species. campylobacter jejuni was recovered from 47.5% of these samples, and giardia species were detected in 82.5%. these findings indicate that muskrat may be of importance to the health both of humans and of domestic animals.19853896141
campylobacter fetus in artificial insemination unit and slaughterhouse bulls in ontario.preputial fluid samples were collected from 90 bulls in two ontario artificial insemination units using a penial glove swab technique previously developed by one of us for use in donor bulls. no campylobacter fetus organisms were identified from the prepuce or from samples of semen collected at the same time from these bulls. the distal genitalia of 200 bulls were collected at a slaughter house. one isolation of a campylobacter fetus subspecies venerealis was obtained on a culture from the forni ...19853893662
thrombophlebitis and cellulitis due to campylobacter fetus ssp. fetus. report of four cases and a review of the literature.four cases of acute thrombophlebitis and cellulitis due to c. fetus ssp. fetus are reported, with a review of 18 previously reported cases. vascular infection with thrombophlebitis due to c. fetus ssp. fetus occurred predominantly in adult male patients with underlying debilitating, immunocompromising illnesses resulting in a mortality rate of 32%. although approximately one-third of the patients had exposure to known reservoirs of c. fetus ssp. fetus, none of the patients presented with diarrhe ...19853892221
isolation, characterization, and serotyping of campylobacter jejuni and campylobacter coli from slaughter cattle.a total of 525 specimens from 100 slaughter beef cattle were examined for the presence of campylobacter jejuni and campylobacter coli by direct plating and enrichment techniques. isolates were identified by cultural, biochemical, antibiotic sensitivity, and immunofluorescence tests and further characterized with the aid of recently developed biotyping and serotyping methods. fifty animals were positive for c. jejuni; only one was positive for c. coli. the distribution pattern of c. jejuni-positi ...19853888109
campylobacter enteritis and bloody stools in the neonate.within 72 hours of birth three babies had loose stools containing fresh blood, mucus, and campylobacter jejuni/coli. campylobacter enteritis should be considered in newborn babies passing blood per rectum.19853874603
comparative in vitro activities of twelve antimicrobial agents against campylobacter species.the in vitro susceptibility of 27 campylobacter jejuni, 31 campylobacter coli, and 30 campylobacter fetus subsp. fetus strains to 12 antimicrobial agents was determined. ciprofloxacin, a new quinoline derivative, was the most active agent tested. antimicrobial susceptibility differed among the three species tested.19853873216
[campylobacter fetus enteritis]. 19853855622
whole milk extender with antibiotics to eliminate campylobacter fetus from frozen bovine semen.fresh bovine semen was contaminated with five strains of campylobacter fetus at 10(5) to 10(6) organisms per ml of semen. the contaminated semen was processed to contain 500 units of polymyxin b, 500 units of penicillin g, and 1000 micrograms of streptomycin sulfate per ml, incubated at 35 degrees c for 10 min, and added then to whole milk extender with like units of antibiotics for further processing. both a low and high initial sperm count requiring extension rates 1:7 and 1:39 were studied. s ...19853842867
in vitro susceptibilities of campylobacter-like organisms to twenty antimicrobial agents.we determined mics of 20 antimicrobial agents for 50 representative strains of four subgroups of campylobacter-like organisms (clos) by agar dilution. ampicillin, gentamicin, doxycycline, tetracycline, ceftriaxone, rifampin, spectinomycin, nalidixic acid, and chloramphenicol were active against all strains of clos. most clo strains (83%) were inhibited by 4 micrograms of sulfamethoxazole per ml and by 8 micrograms of trimethoprim-sulfamethoxazole per ml. of type 1 strains, 28% were resistant to ...19853834828
[septicemia caused by enteritis due to campylobacter fetus subspecies fetus].in a 55 year old man with renal insufficiency requiring hemodialysis after bilateral nephrectomy who had been hospitalized with febrile illness and enteritis, campylobacter fetus subspecies fetus could be isolated from the blood and feces. antibodies against the homologous strain (isolated from the blood) could be detected in the serum by means of the widal test and the indirect immunofluorescence test. culturing of campylobacter fetus subspecies fetus (especially from blood cultures) may be dif ...19873829936
pathogenesis of campylobacter fetus infections: serum resistance associated with high-molecular-weight surface proteins.campylobacter fetus subspecies fetus causes both systemic and diarrheal illnesses. we studied 38 strains of c. fetus isolated from 34 patients; underlying illness was present in eight (89%) of nine patients with only systemic isolates compared with three (20%) of 15 patients with only fecal isolates (p = .002). in a standardized assay of susceptibility to normal human serum, 27 (71%) strains were resistant, six (16%) had intermediate susceptibility, and five (13%) were serum sensitive. major pro ...19873819475
demonstration of a flagellar antigen shared by a diverse group of spiral-shaped bacteria that colonize intestinal mucus.western blot analysis showed that there is little immunological cross-reactivity of the human pathogens campylobacter jejuni and campylobacter coli with campylobacter pyloridis, except for a very strong cross-reaction between the flagellins. this same antigenic cross-reaction was found with two isolates of gram-negative spiral microaerophilic bacteria that are known to colonize the intestinal mucosa of rodents, but not with the flagellins of a number of other motile bacteria. it is proposed that ...19873818099
treatment of campylobacter gastroenteritis.twin boys suffered from recurrent diarrhoea due to campylobacter jejuni after entering a day nursery. stool sampling of all 17 children at the nursery revealed c. jejuni in 12. simultaneous treatment with antibiotics of all children with positive cultures successfully eradicated the infection.19873813645
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