PMID(sorted descending)
medium for isolating campylobacter fetus. 19836649358
morphological differences in flagella in campylobacter fetus subsp. intestinalis and c. fetus subsp. jejuni.intact flagella were isolated from human pathogenic strains of campylobacter, c. fetus subsp. intestinalis and c. fetus subsp. jejuni, by the method of depamphilis and adler and examined by electron microscopy. the isolated flagella were composed of a filament, a hook, a basal body, and a large disk associated with the end of the hook region covering the basal body. the width of the hook was approximately 28 nm, somewhat greater than that of the filament (20 nm in diameter). the hook region of c ...19836645983
[ulcerative colitis and campylobacter fetus ss. jejuni]. 19836645707
milk-borne campylobacter enteritis in a rural area.during november and december 1981 more than 50 residents in a village in derbyshire had an acute gastrointestinal illness. one month later a second outbreak occurred affecting another 22 people. campylobacter jejuni was isolated from 12 patients; no other gastrointestinal pathogens were identified. a case-control study showed an association with the consumption of unpasteurized milk from one particular farm. no new cases were identified for 6 months following the application of a pasteurization ...19836644009
the isolation of campylobacter jejuni from flies collected from three locations were cultured on selective medium for campylobacter spp. campylobacter jejuni was isolated from five (2.4%) of 210 flies examined. these results suggest that the potential hazard to health from the transmission of campylobacters from animals to human food by flies is small.19836644008
probable campylobacter fetus subsp. fetus gastroenteritis.three strains of campylobacter fetus subsp. fetus isolated from cases of gastroenteritis are reported. dna-dna hybridizations in addition to biochemical tests were used to confirm the identification of the isolates as c. fetus since all strains grew at 42 degrees c. these isolates, like other c. fetus strains, are susceptible to cephalothin and thus would not have been detected in laboratories with campylobacter isolation media containing this component.19836643676
campylobacter fetus ssp. fetus bacteremia in a patient with liver cirrhosis. 19836642681
simple adult rabbit model for campylobacter jejuni enteritis.we tested the usefulness of the removable intestinal tie adult rabbit diarrhea model to establish campylobacter jejuni infection in rabbits. the procedure involved ligation of the cecum, placement of a slip knot at the terminal ileum, and injection of the test inoculum into the mid-small bowel. the ends of the slip knot were externalized, and the tie was released 4 h later. fifty-five rabbits received c. jejuni, and 16 received uninoculated medium as controls. daily rectal swabs were positive fo ...19836642664
molecular identification of surface protein antigens of campylobacter jejuni.the technique of immunoblotting was used to identify the surface protein antigens of campylobacter jejuni. polyclonal antisera were raised in rabbits to formalinized cells of a typical human fecal isolate, c. jejuni vc74. surface components were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. fractions analyzed included whole cell lysates, sarcosinate-extracted outer membranes, released outer membrane blebs (fragments), isolated flagella, 0.2 m glycine-hydrochloride (ph 2 ...19836642648
demonstration of a cytotoxin from campylobacter jejuni.a 48-hour culture filtrate of campylobacter jejuni was found to produce cytopathic effects on three human cell lines--that is, hela, mrc-5 and hep-2. the cytopathic effects observed include cell rounding, loss of adherence and cell death after 24-48 h of incubation. such morphological changes were observed with eight of the eleven strains of campylobacter jejuni isolated from the blood/stools of patients who suffered from either acute gastroenteritis or septicaemia. the toxic factor did not reta ...19836630575
campylobacter fetus subsp. fetus in homosexual males.campylobacter fetus subsp. fetus was isolated from the stools of two homosexual males. one was asymptomatic at the time of isolation. the other presented with diarrhea. both isolates were initially grown at 42 degrees c. this organism should be included among the list of organisms that are found in homosexual males.19836630480
effect of ferrous sulfate, sodium metabisulfite, and sodium pyruvate on survival of campylobacter jejuni.a combination of ferrous sulfate, sodium metabisulfite and sodium pyruvate, incorporated in solid medium, maintained the characteristic morphology, motility, and viability of six isolates of campylobacter jejuni stored at room temperature and 4 degrees c for up to 20 and 30 days, respectively, under normal atmospheric conditions.19836630476
enhanced isolation of campylobacter jejuni by cold enrichment in campy-thio broth.isolation of campylobacter jejuni from human feces by direct inoculation to campy-bap (scott laboratories, inc., fiskeville, r.i.) was compared with isolation after overnight enrichment at 4 degrees c in campy-thio broth followed by subculture to campy-bap. of 54 positive specimens, 19 were positive only after enrichment, and 5 were positive only on the direct plate. among 36 positive patients, 10 were detected by enrichment only and 2 by direct plating only. laboratories using campy-bap should ...19836630456
isolation of campylobacter jejuni from raw milk.campylobacter jejuni was isolated from raw milk by a method that can routinely detect less than or equal to 1 organism per ml. this procedure was used in a survey of 195 separate farms and showed a 1.5% incidence of c. jejuni in milk from bulk tanks.19836625570
[erythema nodosum and campylobacter fetus infection]. 19836625463
inhibitory and lethal activities of rosaramicin, erythromycin, and clindamycin against campylobacter fetus subsp jejuni and a comparative study of the inhibitory and lethal effects of rosaramicin, erythromycin, and clindamycin on strains of campylobacter fetus subsp jejuni and c intestinalis, c jejuni was more readily killed by rosaramicin and clindamycin than was c intestinalis. erythromycin exhibited an equally lethal effect against both subspecies. however, it was the least active of the macrolides tested against both c jejuni and c intestinalis.19836625312
[occurrence of campylobacter fetus subsp. jejuni in mice and guinea pigs from experimental animal establishments]. 19836624307
electron microscopy of the coccoid form of campylobacter jejuni.confluent cultures of campylobacter jejuni incubated for 24 and 48 h each were examined by electron microscopy. although the 24-h-old cells exhibited typical curved morphology, the 48-h-old cells showed rounded morphology with a loss of cell integrity. this appeared to be an autolytic process that occurred very rapidly after the culture became mature. these results confirm previous evidence that the coccoid form of this organism is a degenerate state.19836619291
extended scheme for serotyping campylobacter jejuni: results obtained in israel from 1980 to 1981.the serotyping scheme for campylobacter jejuni previously developed in the national center for campylobacter, jerusalem, was extended by the use of 20 new sera and modified by the absorption of the sera, when necessary, with homologous boiled cultures or heterologous live cultures. the extended scheme is based on slide agglutination of live suspensions and is performed in two stages: pretesting with four pooled sera and final testing with monovalent sera. so far, 34 serotypes have been recognize ...19836619282
identification and characterization of campylobacter jejuni outer membrane proteins.outer membrane proteins from isolates of campylobacter jejuni were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. sarcosinate-insoluble membrane preparations were outer membrane enriched based on increased ketodeoxyoctonate concentrations, the presence of surface-exposed 125i-labeled proteins that were hydrophobic, and similarity to membrane vesicle (bleb) sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles. most isolates contained a single major band with ...19836618667
[campylobacter fetus bacteremia]. 19836618327
campylobacter-induced toxic megacolon.a case of a compromised host with myeloproliferative syndrome who presented with severe, relentless but nonbloody diarrhea, fever, severe colitis, and toxic megacolon is presented. blood cultures grew out campylobacter fetus ssp. fetus. specific treatment with erythromycin reversed the grave clinical picture to normal in a 5-day period.19836613968
a case of a double infection with salmonella typhi and campylobacter fetus subspecies jejuni.a case of a double infection with salmonella typhi and campylobacter subspecies jejuni proven by isolation and serology is reported. the patient was effectively treated with erythromycin and chloramphenicol.19836612785
hla-b27-negative arthritis related to campylobacter jejuni enteritis in three children and two adults.five out of 37 patients with proven campylobacter jejuni enteritis developed arthritis. two adult patients presented with classical reiter's syndrome. one of the three children had reactive arthritis, and clinical suspicion of septic arthritis could not be confirmed in two. the acute synovitis subsided usually without treatment in all patients within 3-7 days, while arthralgia persisted longer in 4 patients. hla-b27 was not present in the 5 patients with arthritis, but was found in 4 others. in ...19836605028
the microbial evaluation of acute cellulitis.twenty consecutive patients with acute cellulitis were studied by retrospective chart review. patients with suffusion and edema, with or without vesicles, were included. patients with breaks in the skin, with focal suppurations or with concurrent antimicrobial therapy were excluded. in nineteen of the twenty patients, aspirates (95 percent) failed to grow organisms. pasteurella multocida was grown from subcutaneous aspirate and blood cultures of a single patient; he was immunocompromised by wald ...19836602690
the effect of storage in liquid nitrogen on the recovery of human dental plaque bacteria.centrifuged deposits of various individual bacteria from type-culture collections and dental plaque samples were immersed in liquid nitrogen for periods of up to 2 years. all the organisms were recovered after storage in liquid nitrogen but fusobacterium nucleatum (atcc 10953) and campylobacter fetus (nctc 10354) consistently showed a reduction in the number of colony-forming units after freezing. the main effect on bacterial proportions in plaque samples was a reduction in aerobic gram-negative ...19846596040
[rotavirus and campylobacter fetus jejuni associated with an outbreak of diarrhea in calves].rotaviruses and campylobacter fetus jejuni are ubiquitous agents of diarrheal disease in animals and humans. under natural conditions they do not seem to cross inter-species barriers; a zoonosis has not been documented for man. however, animal rotaviruses might contribute to the emergence of new reassortment strains in view of their segmented genome, and thus, produce new antigenic variants. on the contrary, campylobacter fetus jejuni produces a true zoonosis. man acquires bacilli by ingesting w ...19846545623
growth and survival characteristics of campylobacter jejuni in liquid egg.growth and survival of four campylobacter jejuni strains in yolk, in liquid whole egg and in white during aerobic storage at 37, 20 and 4 degrees c was followed. in 48 h at 37 degrees c the cell counts of c. jejuni increased by about 3 log10 units in yolk and 1.60-3.35(10) log units in liquid whole egg. the growth of c. jejuni was slightly better in yolk than in liquid whole egg. at 20 degrees c during 48 h the cell counts decreased by about 0.5-1.5 log10 units in yolk and in liquid whole egg. a ...19846537959
[campylobacter fetus subsp. jejuni: etiological, epidemiological and preventive aspects].the authors, after a brief introduction, examine on the basis of the most recent literature, the taxonomy and the microbiological features of campylobacter fetus subsp. jejuni, giving a detailed description of the chemical composition, of the biochemical, physiological and nutritional characters, of the antigenic composition and of the cultural methods. later on, they state the problems arising from the epidemiology of the infections caused by this germ either in man or in animals, examining the ...19846536547
[diarrhea caused by campylobacter fetus jejuni and other infective agents in children of the rural area of puriscal, costa rica].between september 1979 and september 1981 a field study was conducted on the etiology of diarrheal disease in the area of puriscal, costa rica. the presence of enteric pathogens was investigated in the stools of 267 diarrheic children and 190 healthy controls. both groups belong to yearly cohorts recruited at birth as part of a longitudinal multidisciplinary study of mothers and children. campylobacter fetus jejuni was identified as the only pathogen in the stools of 24 diarrheic children (9%) a ...19846535179
levels of some reproductive diseases in the dairy cattle of colombia.of 4,144 serum samples collected from cows on 113 farms from eight areas of colombia 3.3% had positive and 8.8% inconclusive titres to brucella abortus, 21.7, 6.3, 1.6, 0.6 and 0.7% of cows had positive titres to leptospira serovars hardjo, pomona, canicola, icterohaemorrhagiae and grippotyphosa respectively. questionnaires completed on 110 farms revealed that 6, 2.5 and 4.6% of cows had had metritis, aborted or retained their placentas respectively in the previous 12 months. trichomonas foetus ...19846523582
composition of the antigenic material removed from campylobacter jejuni by heat.the antigenic material removed form campylobacter jejuni by the boiling of whole cells in saline was examined biochemically. analyses showed that the extracted material contained 3 micrograms of protein per ml per mg of wet cells and ca. 2.6 micrograms of carbohydrate per ml per mg of wet cells. further extraction of the material with chloroform-methanol produced about 0.5 microgram of water-insoluble residue per ml per mg of wet cells, suggesting the presence of lipid as well. additional analys ...19846520219
antibacterial activities of nitrothiazole against campylobacter jejuni and campylobacter coli.niridazole (ambilhar) and three other newly synthesized nitrothiazole derivatives were highly active against 19 microaerophilic campylobacters (minimum concentration required to inhibit 50% of strains [mic50], 0.0075 to 0.015 mg/liter). there were, however, considerable differences in the susceptibility among strains tested, and one nitrothiazole derivative was rather inactive (mic50, 2 mg/liter). nitroimidazole derivatives, such as metronidazole and tinidazole, were less active (mic50, 2 and 4 ...19846517542
serotyping and serology studies of campylobacteriosis associated with consumption of raw milk.a community outbreak of 15 cases of gastroenteritis was traced to consumption of unpasteurized milk produced at one commercial dairy. using two different testing schemes, we found that a campylobacter jejuni isolate from an ill patient and an isolate from a sick cow were the same serotype. bacteriological studies suggested that a single epidemic strain of campylobacter jejuni caused this outbreak.19846511884
improved biotyping schemes for campylobacter jejuni and campylobacter coli.campylobacter jejuni (20 strains) and campylobacter coli (12 strains) were assigned to four biovars for each species based on phenotypic tests that were easy to perform and interpret. the resulting biotyping schemes offer a greater degree of distinction among c. jejuni and c. coli strains than any of the other biotyping schemes previously described for these organisms.19846511880
comparison of antimicrobial susceptibility patterns of campylobacter jejuni and campylobacter determine whether employing antibiograms is useful to separate campylobacter jejuni and campylobacter coli, we determined the mics of 12 antibiotics for 104 human clinical strains and 74 swine strains. of 74 swine strains, 5 (7%) were hippurate positive, as were 93 (89%) of 104 human strains. the 12 antimicrobial agents tested were ampicillin, amoxicillin, clindamycin, chloramphenicol, erythromycin, furazolidone, norfloxacin, nalidixic acid, rosoxacin, rosaramicin, tetracycline, and sch 32063 ...19846508265
quantitative evaluation of a transport-enrichment medium for campylobacter fetus.the enrichment feature of a selective serum-based transport medium for campylobacter fetus was quantitatively examined. preputial samples from artificial insemination bulls were spiked with known numbers of c fetus strains and inoculated into transport-enrichment medium (tem). the survival and multiplication of these strains in tem under different incubation periods and temperatures were assessed by plate counts. mean enrichment values of 3.72 log and 4.42 log were observed after incubation at 3 ...19846506432
a search for the source of campylobacter jejuni in milk.samples of milk from 1501 cows with mastitis were negative for campylobacter jejuni. the faeces of 74 healthy friesian cows were screened for c. jejuni: 13% of the samples were positive during the summer when the cows were on pasture, and 51% were positive in the winter when the cows were housed. positive samples contained on average 1 x 10(4) campylobacters per g faeces. it is concluded that faecal contamination rather than udder infection is the means by which campylobacters enter milk and the ...19846501879
epidemiological investigations on campylobacter jejuni in households with a primary infection.fifty-four rotterdam patients in which a primary infection with campylobacter jejuni had been detected (index patients) were compared with 54 control subjects with regard to the consumption and preparation of foods 7 days before onset of illness and the keeping of pet animals. significantly more index patients than controls had eaten chicken meat (47 v. 29; p = 0.0002), particularly at barbecues (14 v. 2; p = 0.0015). marginally more index patients had eaten pork (47 v. 39; p = 0.048) or inadequ ...19846501878
use of an ammonia electrode to study bacterial deamination of amino acids with special reference to d-asparagine breakdown by campylobacters.a method using an ammonia electrode is being developed for investigating the deamination of amino acids and amides by bacteria. application of this method to campylobacter jejuni and c. coli has led to the demonstration of d-asparaginase activity in some strains. this has allowed the subdivision of both species into d-asparaginase-positive and -negative biotypes. even though the method is in the developmental stage, it was found to be generally reproducible and easy to perform. areas for further ...19846501873
heat injury and repair in campylobacter jejuni.a procedure for detecting and quantitating heat injury in campylobacter jejuni was developed. washed cells of c. jejuni a7455 were heated in potassium phosphate buffer (0.1 m, ph 7.3) at 46 degrees c. samples were plated on brucella agar supplemented with na2s2o3, feso4 x 7h2o, and sodium pyruvate and on a medium containing brilliant green, bile, na2s2o3, feso4 x 7h2o, and sodium pyruvate. colonies were counted after 5 days of incubation at 37 degrees c in an atmosphere containing 5% o2, 10% co2 ...19846497368
new, extended biotyping scheme for campylobacter jejuni, campylobacter coli, and "campylobacter laridis".a biotyping scheme using improved media and methods for the detection of hippurate hydrolysis, rapid h2s production, and dna hydrolysis was applied to 1,826 cultures of campylobacter jejuni, campylobacter coli and "campylobacter laridis" isolates from human and nonhuman sources. four biotypes were identified among c. jejuni: 57.3% of the isolates belonged to biotype i; 36.0%, to biotype ii; 4.0%, to biotype iii; and 2.7%, to biotype iv. c. coli organisms were differentiated into biotype i (67.0% ...19846490850
campylobacter fetus ssp jejuni as seen in bangalore. 19846490113
susceptibility of campylobacter fetus subsp. jejuni to paromomycin. 19846489324
differential characteristics of catalase-positive campylobacters correlated with dna homology groups.eighty-four strains of catalase-positive campylobacters could be placed into seven distinct dna homology groups (species), corresponding to campylobacter fetus, "c. hyointestinalis," c. jejuni, c. coli, "c. laridis," "c. fecalis," and aerotolerant campylobacters. the biochemical and physiological characteristics of the strains were examined for their correlation with the homology groups. the characterization tests that provided the most reliable differentiation at the species and subspecies leve ...19846478314
selected enrichment broths for recovery of campylobacter jejuni from foods.we attempted to shorten the required time for enrichment broth culture for the isolation of campylobacter jejuni. enrichment broths described by doyle and roman and park and stankiewicz and one developed during this study were compared for ability to isolate c. jejuni from raw chicken carcasses. our medium was a modification of that of doyle and roman with the addition of filter-sterilized fbp (0.2% ferrous sulfate, 0.025% sodium metabisulfite, 0.05% sodium pyruvate), 0.1% sodium lauryl sulfate, ...19846476833
campylobacter enteritis on hopi and navajo indian reservations. clinical and epidemiologic features.from june 22 through september 30, 1981, stool specimens from 522 hopi and navajo outpatients were cultured because of diarrheal illnesses at the keams canyon indian health service hospital, arizona. campylobacter jejuni was isolated from the specimens of 26 (5%) of the patients. this pathogen was found as frequently as shigella in patients younger than 2 years or older than 20 years, but was significantly less common in the 2 to 20-year age group (p<.000001). campylobacter enteritis was indisti ...19846475040
a new bacterial flagellar structure found in campylobacters.a previously unrecognized bacterial flagellar structure present in campylobacter jejuni and campylobacter fetus is described. the structure is located just below the insertion of the flagellar apparatus through the bacterial wall. it is disc shaped, approximately 100 nm in diameter and has eleven spokes radiating from a central hole. the function of this new structure and its possible importance in the taxonomy of campylobacters and other spiral micro-organisms are discussed.19846470668
isoprenoid quinone content and cellular fatty acid composition of campylobacter species.a total of 36 strains of campylobacter species were examined for isoprenoid quinones and cellular fatty acids. the isoprenoid quinone content was determined by reverse-phase high-pressure liquid chromatography, and the fatty acids were determined by capillary gas-liquid chromatography. all campylobacter species contained menaquinone-6 (2-methyl-3-farnesyl-farnesyl-1,4-naphthoquinone) and a methyl-substituted menaquinone-6 (2,[5 or 8]-dimethyl-3-farnesyl-farnesyl-1,4-napthoquinone) as the major i ...19846470096
campylobacter fetus subspecies jejuni in senegal. 19846468362
a study of campylobacter fetus subsp. jejuni strains isolated from the stools of diarrhoeic children in sri lanka.nine strains of campylobacter fetus subsp. jejuni were isolated from the stools of diarrhoeic children in colombo (sri lanka). the morphology of most of the strains was typical but three strains produced a spreading film of growth. no discrete domed colonies were seen. all were able to grow in a candle jar, and in a medium containing 1% glycine. all produced hydrogen sulphide. all were susceptible to ampicillin, carbenicillin, tetracycline, chloramphenicol, neomycin, erythromycin, gentamicin and ...19846464104
spontaneous bacterial peritonitis due to campylobacter jejuni.a patient with alcoholic cirrhosis complicated by ascites developed spontaneous bacterial peritonitis. campylobacter jejuni--(a microaerophilic bacillus)--was isolated from ascitic fluid and blood.19846463002
in vitro susceptibility of campylobacter fetus subsp. jejuni to n-formimidoyl thienamycin, rosaramicin, cefoperazone, and other antimicrobial agents.the activities of 11 antimicrobial agents against 36 strains of campylobacter fetus subsp. jejuni were studied by a broth microdilution method. all strains were susceptible to 7 of the 11 antimicrobial agents. of the newer agents tested n-formimidoyl thienamycin (mk0787) and rosaramicin had very good activity, whereas cefotaxime, moxalactam, and cefoperazone had poorer activity.19816459767
[study of bacterial motility and rate of movement using a closed circuit television].speed and motion patterns of campylobacter fetus ssp. jejuni, escherichia coli and pseudomonas aeruginosa were recorded using a closed circuit television camera attached to a phase contrast microscope. a sony video analysis system was used to stop frame videotape at 1/7th and 1/15th. bacterial speeds were: campylobacter 29.2 micron/s, e. coli 8.9 micron/s and p. aeruginosa 16.8 micron/s.19846443639
economical, simple method for production of the gaseous environment required for cultivation of campylobacter jejuni.campylobacter jejuni is an enteric pathogen recognized worldwide as a cause of diarrhea. its isolation from stool samples requires a microaerophilic environment that heretofore has been expensive and cumbersome to create. an economical, portable, and simple method is described which involves the production of appropriate concentrations of oxygen and carbon dioxide. inside a plastic bag are placed two cups, one containing fine steel wool (grade 0) previously soaked in a 2.5% aqueous solution of c ...19846436301
[campylobacter fetus meningitis in an adult male]. 19846434669
premature labor and neonatal sepsis caused by campylobacter fetus, subsp. fetus--ontario. 19846431256
guillain-barré syndrome and campylobacter jejuni: a serological study.the association between campylobacter jejuni infection and guillain-barré syndrome was investigated serologically in a retrospective study of 56 patients admitted to this hospital over four years. evidence of preceding c jejuni infection was found in 21 (38%) of these patients, indicating that c jejuni was the most common single identifiable pathogen precipitating the disease. among those patients who had presented with preceding diarrhoea the serum antibody response was similar to that in uncom ...19846428580
structure and cell envelope associations of flagellar basal complexes of vibrio cholerae and campylobacter isolate intact flagella with basal complexes from vibrio cholerae, a rhamnolipid hemolysin from pseudomonas aeruginosa was used to disrupt the cell envelope and flagellar sheath. the nonionic detergent, triton x-100, provided similar results for campylobacter fetus. each of these basal complexes possessed, in addition to the four classical rings, concentric membrane rings (cmr's) similar to those found in aquaspirillum serpens. through the use of stereo imaging (which allows structures to be ...19846426766
a study of the oxygen and carbon dioxide requirements of thermophilic campylobacters.the oxygen and carbon dioxide requirements of different biotypes of thermophilic campylobacters were investigated by means of (a) quantitative studies, and (b) total growth studies. oxygen tolerance of the five test organisms differed markedly and varied with the carbon dioxide concentration. at most carbon dioxide concentrations tested, campylobacter jejuni strains nctc 11168 and nctc 11392 tolerated 21% oxygen (growth reduced), c coli nctc 11353 tolerated 15% oxygen (growth reduced), and c jej ...19836408142
biphasic culture system for rapid campylobacter cultivation.we developed a biphasic culture system consisting of 4 ml of brucella agar (ba) and 6 ml of brucella broth (bb) in 25-cm2 tissue culture flasks, which were incubated in air (bb/baa) or in a gas mixture of 5% o2, 10% co2, and 85% n2 (bb/bag). these media were also used with a supplement consisting of ferrous sulfate, sodium metabisulfite, and sodium pyruvate and incubated as above (fb/faa and fb/fag, respectively). highly satisfactory growth of campylobacter jejuni 301 was obtained with all mediu ...19836401980
isolation of campylobacter fetus ss jejuni in adult patients with diarrheal syndrome. 19846399196
comparison of enrichment methods and atmosphere modification procedures for isolating campylobacter jejuni from foods.a comparison was made of enrichment broths for recovery of campylobacter jejuni from food by the methods of doyle and roman (appl. environ. microbiol. 43:1343-1353) and of park et al. (can. j. microbiol. 27:841-842). no significant differences were found between the results obtained with the two broths. recovery was greater, however, with a constant gas flow into the broths than with an evacuation-replacement method.19846393876
intestinal carriage of campylobacter jejuni and salmonella by chicken flocks at slaughter.campylobacter jejuni were isolated in large numbers from the majority of birds sampled in colonic swabs from 28 of 60 flocks at slaughter. by contrast only small numbers of birds from 11 of the same 60 flocks yielded salmonella enteritidis serotypes. three c. jejuni isolates from each flock were serotyped on the basis of their heat-stable antigens, using antisera prepared against 16 serotypes common in campylobacter diarrhea in man. the majority (72 of 83) of the chicken isolates could be seroty ...19846383579
detection of campylobacter by immunofluorescence in stools and rectal biopsies of patients with diarrhoea.rabbit antiserum, elicited by the intravenous injection of a strain of campylobacter jejuni heated to 100 degrees c, cross reacted strongly with all other thermophilic campylobacters tested as well as with "c pyloridis" and could be detected by indirect fluorescence with labelled anti-rabbit serum. antisera to formalin killed cells did not do so. the correlation of positive stool culture with positive immunofluorescence of stools and rectal biopsies from patients with diarrhoea was 70-80%. some ...19846381550
campylobacter fetus ssp fetus cholecystitis and relapsing bacteremia in a patient with acquired immunodeficiency syndrome.acute gangrenous cholecystitis and relapsing bacteremia caused by campylobacter fetus ssp fetus occurred in a patient with acquired immunodeficiency syndrome. biliary tract colonization probably accounted in part for this unusual phenomenon. whether the patient's deficiency of cell-mediated immunity contributed to his disease is not known.19846377511
[an optimal blood culture technic].for blood cultures in septicemic patients it is advisable to use two broth culture bottles. one bottle should be incubated with air, the other without (for anaerobes). supplemented peptone broth ii (becton-dickinson) and bhi roche were superior for culturing aerobic and anaerob bacteria in comparison to other blood culture media. they are commercially available, and with these media it is not necessary to inoculate two bottles with blood from one patient. supplemented peptone broth ii is also su ...19846374431
procedure for increased recovery of campylobacter jejuni from inoculated unpasteurized milk.different treatments were applied to campylobacter jejuni-inoculated unpasteurized milk to identify means of enhancing the survival of the organism in refrigerated (4 degrees c) samples. the greatest survival occurred in milk supplemented with 0.01% sodium bisulfite and held under an atmosphere of 100% nitrogen (bisulfite-nitrogen), in most instances allowing isolation of c. jejuni from highly contaminated milk 15 or more days longer than from unsupplemented milk held in air (21% oxygen). althou ...19846370137
is enrichment culture necessary for the isolation of campylobacter jejuni from faeces? 19846368607
analysis of campylobacter jejuni antigens with monoclonal develop monoclonal reagents for antigenic analysis and serotyping of campylobacter spp., hybridoma cell lines were produced by fusion of mouse myeloma cells and spleen cells from mice immunized with formalin-treated campylobacter jejuni organisms. an enzyme immunoassay was used for preliminary screening of the cell culture supernatants and ascites. twenty-nine clones which reacted with the immunogen were obtained. seven of these clones were positive in passive hemagglutination tests with shee ...19846365954
campylobacter jejuni outer membrane proteins are antigenic for humans.all campylobacter jejuni strains have a major outer membrane protein (omp) that migrates between a molecular weight of 41,000 (41k) and 45k and represents more than 50% of protein present, plus several more minor bands. using 125i-radiolabeled c. jejuni cells in a radioimmunoprecipitation procedure to assess whether the omps were antigenic, we studied serum from rabbits immunized with c. jejuni cells, from humans convalescent after c. jejuni infection, and from appropriate controls. in this assa ...19846365789
enteropathogen carriage by healthy individuals living in an area with poor sanitation.faecal carriage of bacterial enteropathogens (enteropathogenic escherichia coli (epec), shigellae and salmonellae) was studied in 265 individuals: 65 infants 3-6 months of age (50 bottle-fed and 15 breast-fed), 100 school-age children 8-10 years of age and 100 adults 21-50 years of age. all were apparently healthy, did not have gastrointestinal symptoms, had not received antibiotics in the preceding fortnight and were not malnourished. enteropathogens were isolated from the faeces of 24 individu ...19836363528
is enrichment culture necessary for the isolation of campylobacter jejuni from faeces?the role of enrichment culture for the isolation of campylobacter jejuni from faeces is discussed. it is concluded that enrichment culture is only necessary for those specimens where it is anticipated that the number of organisms is likely to be low. in a trial of a blood free enrichment broth (ccd broth) and the modified preston enrichment broth the latter gave significantly superior results.19836361073
evaluation of filters for recovery of campylobacter jejuni from water.campylobacter jejuni has been incriminated in several large waterborne outbreaks, but it has rarely been isolated from water itself. better methodology is needed for the isolation of c. jejuni from water. we evaluated three types of 0.45-micron microporous filters and three different pore sizes of positively charged depth filters for their ability to recover c. jejuni from seeded, sterile tap and surface water. the microporous filters tested were millipore ha, gelman gn6, and zetapor. three pore ...19836360048
correlation of an expanded direct fluorescent-antibody system with an established passive hemagglutination system for serogrouping strains of campylobacter jejuni and campylobacter coli.rabbits were inoculated with whole, formalinized cells from eight passive hemagglutination reference strains of campylobacter. fluorescein isothiocyanate-labeled immunoglobulin g from these antisera defined seven new direct fluorescent-antibody serogroups of c. jejuni and one new serogroup of c. coli. this expanded the campylobacter direct fluorescent antibody system to include 17 serogroups of c. jejuni, 3 serogroups of c. coli, and 2 serogroups of c. fetus. we then compared the passive hemaggl ...19836358249
[an infection caused by campylobacter fetus]. 19836357724
detection of campylobacter fetus in artificial insemination bulls with a transport enrichment hundred and five bulls from an artificial insemination unit were tested for the presence of campylobacter fetus subspecies venerealis. the method involved the inoculation of preputial samples into a new transport enrichment medium prior to culture and immunofluorescence tests. seventeen bulls (16%) were found to be either positive or suspected carriers of c. fetus at one or more sampling times. the average age of these 17 bulls was about two years greater than the average age of all the bull ...19836357410
evaluation of transport media for campylobacter jejuni in human fecal is not always possible to culture feces immediately, and appropriate methods for transport of human specimens, unlike those from animals, have not been fully evaluated. therefore, we took serial subcultures in two phases from six transport media inoculated with human diarrheal stools known to be positive for campylobacter jejuni. in phase 1, cary-blair medium and buffered glycerol saline did not preserve c. jejuni as well as did alkaline peptone-water (apw), modified cary-blair medium, thiogl ...19836355160
isolation of campylobacter jejuni from an appendix.campylobacter jejuni skirrow biotype 1, lior serotype 8 was isolated from the appendix of an 11-year-old boy who had a 6-h history of acute abdominal pain. histological diagnosis on the appendix section was early acute appendicitis. dilute carbol fuchsin stain and indirect fluorescent antibody test performed on the appendix section also revealed the presence of campylobacter sp. the patient developed a significant bactericidal antibody titer of 1,024, providing substantial clinical evidence of t ...19836352736
[campylobacter infection in children].enteritis by campylobacter jejuni/coli is by far the most frequent clinical entity of campylobacter-infection. it may be compared to salmonella-enteritis as for its epidemiologic importance. the course of the disease may be dramatic, but can be successfully treated as soon as the pathogen is known. in prematures and newborn babies infections with campylobacter fetus ssp. fetus are life-threatening and early diagnosis is of life-saving importance. five observations of campylobacter-enteritis in c ...19836352463
an acid extract as a common antigen in campylobacter coli and campylobacter jejuni acid extract prepared from a single strain of campylobacter jejuni reacted with rabbit antisera against 21 strains of c. jejuni and campylobacter coli. the immunogen is resistant to formalin and to exposure to 100 degrees c for 30 min. the antigenic extract may have practical applications in human serology.19836343426
serogroups of campylobacter jejuni, campylobacter coli, and campylobacter fetus defined by direct immunofluorescence.rabbits were inoculated with whole, formalinized campylobacter jejuni, c. coli, and c. fetus cells; the c. jejuni and c. coli immunogens were identified by their dna relatedness at the species level to the type strains of c. jejuni or c. coli. the designation c. coli was not used among the other c. jejuni strains; they were classified as hippurate-positive or hippurate-negative c. jejuni. immunoglobulin g was isolated from the antisera and labeled with fluorescein isothiocyanate. these conjugate ...19836341405
avian wildlife reservoir of campylobacter fetus subsp. jejuni, yersinia spp., and salmonella spp. in norway.cloacal swabs from 540 wild-living birds were cultured for campylobacter fetus subsp. jejuni, yersinia spp., and salmonella spp. the carrier rates detected were as follows: c. fetus subsp. jejuni, 28.4%; yersinia spp., 1.2%; and salmonella spp., 0.8%. all birds were apparently healthy when captured. c. fetus subsp. jejuni was isolated from 11 of the 40 bird species examined. among birds inhabiting the city of oslo, the highest isolation rate was found in crows (corvus corone cornix) (89.8%), fol ...19836338824
diarrhea associated with rotaviruses, enterotoxigenic escherichia coli, campylobacter, and other agents in costa rican children, 1976-1981.rotaviruses, enterotoxigenic escherichia coli, salmonella, shigella, and parasites were investigated in outpatient diarrheic children, and in hospitalized diarrheic and non-diarrheic children, between january 1976 and june 1979. in outpatient cases studied within 4 days of onset of symptoms, rotaviruses were the most common agents (45.3%); e. coli heat-stable enterotoxin ranked second (13.4%); shigella was third (8.1%); salmonella was fourth (7.3%). in 63.2%, one or more enteric agents were dete ...19836337517
parasitic, bacterial, and viral enteric pathogens associated with diarrhea in the central african republic.a total of 1,197 diarrheic children less than 15 years old were investigated for parasitic, bacterial, and viral enteropathogens from march 1981 through february 1982 in the central african republic. one or more pathogens were identified from 49.4% of the patients. rotavirus was the most frequently identified pathogen among children less than 18 months old. enteropathogenic escherichia coli was the second most frequently isolated pathogen (12.1%) in children less than 2 years of age. campylobact ...19846330161
investigation of a campylobacter jejuni outbreak by serotyping and chromosomal restriction endonuclease analysis.fifty campylobacter jejuni isolates, including 29 from humans associated with an outbreak of enteritis, 20 from cattle, and 1 from a milk source, were serotyped on the basis of extractable thermostable antigens and examined by bacterial chromosomal restriction endonuclease digest analysis. serotyping showed specific differences between the human isolates and the milk isolates, but each of these generally, although not consistently, reacted with 4 of the 42 c. jejuni typing antisera. restriction ...19846325487
[campylobacter fetus fetus bacteremia. review of the literature apropos of 2 cases].the authors report two cases of bacteremia due to campylobacter fetus fetus (also called c. fetus intestinalis). one was a 22-year-old female, under treatment for active systemic lupus erythematosus, who died. the other was a fifty-year-old male, in a poor general condition, who had a self-limited acute febrile dysentery. in this patient, c. fetus fetus was found in stools after apparent recovery, raising the problem of healthy carriers. a review of the literature shows 64 other such cases.19836320419
characterization of tetracycline resistance plasmids from campylobacter jejuni and campylobacter coli.tetracycline resistance in strains of campylobacter jejuni and campylobacter coli was mediated by plasmids. intra- and interspecies transfer was demonstrated within the genus campylobacter. buoyant densities of plasmid dnas ranged from 1.691 to 1.694 g/cm3 (31 to 33% guanine plus cytosine). restriction enzymes acci, bcli, bglii, and psti were found to be most useful for comparing the plasmids. the molecular weight of c. jejuni plasmid pmak175 was 44.7 kilobases (29 x 10(6), and the other plasmid ...19836318666
application of serotyping and chromosomal restriction endonuclease digest analysis in investigating a laboratory-acquired case of campylobacter jejuni enteritis.a frequently passaged laboratory strain of campylobacter jejuni was confirmed by serotyping on the basis of thermostable antigens and by bacterial chromosomal restriction endonuclease digests to be the causative agent of enteritis in a laboratory worker.19836317712
the polymicrobial origin of intestinal infections in homosexual determine the microbial cause and the clinical and pathologic correlates of anorectal and intestinal symptoms in homosexually active men, we performed comprehensive microbiologic studies, anoscopy, sigmoid-oscopy, and rectal biopsy in men examined in a clinic for sexually transmitted diseases. enteric pathogens were found in 95 of 119 consecutive homosexual men with anorectal or intestinal symptoms and in 29 of 75 randomly selected homosexual men without such symptoms (p less than 0.001). the ...19836308444
value of stool examination in patients with diarrhoea.findings of stool examinations in 1593 patients with diarrhoea due to a single enteric pathogen--enterotoxigenic escherichia coli rotavirus, shigella, campylobacter jejuni, vibrio cholerae 0:1, entamoeba histolytica, or giardia lamblia--were reviewed to determine how well they predicted the agent associated with the diarrhoea. specimens were examined visually for blood and mucus, tested for ph, and examined under a microscope for the presence of red and white blood cells, parasites, and stool fa ...19836307460
effects of disinfectants on campylobacter jejuni.because of the increasing recognition that campylobacter jejuni is an important enteric pathogen of humans, we studied the effects of widely used disinfectants on the viability of this organism. at an inoculum size of 10(3) to 10(4) cfu/ml, 1.25 mg of hypochlorite per liter killed three strains within 1 min. at an inoculum size of 10(6) to 10(7) cfu/ml, 5 mg of hypochlorite per liter killed three strains within 15 min. killing of similar concentrations of c. jejuni and escherichia coli by hypoch ...19836305266
aerobic and anaerobic respiratory systems in campylobacter fetus subsp. jejuni grown in atmospheres containing hydrogen.maximum growth of campylobacter fetus subsp. jejuni, strain c-61, occurred when the cultures were incubated with shaking in atmospheres containing approximately 30% hydrogen, 5% oxygen, and 10% co2. suspensions of cells grown under these conditions consumed oxygen with formate as the substrate in the presence of 0.33 mm cyanide, which completely inhibited respiration with ascorbate-n,n,n',n'-tetramethyl-p-phenylenediamine and with lactate. spectroscopic evidence with intact cells suggested that ...19826288661
respiratory physiology and energy conservation efficiency of campylobacter jejuni.a study of the electron transport chain of the human intestinal pathogen campylobacter jejuni revealed a rich complement of b- and c-type cytochromes. two c-type cytochromes were partially purified: one, possibly an oxidase, bound carbon monoxide whereas the other, of high potential was unreactive with carbon monoxide. respiratory activities determined with membrane vesicles were 50- to 100-fold higher with formate and hydrogen than with succinate, lactate, malate, or nadh as substrates. evidenc ...19826277867
the relative importance of rotavirus and other pathogens in the etiology of gastroenteritis in trinidadian children.over a 2-year period, 300 infants less than 3 years old with gastroenteritis admitted to hospitals in trinidad were investigated for the presence of certain microorganisms in the feces, along with an equal number of age- and sex-matched controls. rotavirus was detected in 23% of cases and 1% of controls; salmonella in 7% of cases and in 1% of controls; shigella in 4% of cases and in no controls and two serotypes of enteropathogenic e. coli in 7% of cases and in 2% of controls. campylobacter fetu ...19826277207
travelers' diarrhea in panamanian tourists in determine whether residents of developing countries are unlikely to acquire travelers' diarrhea, 64 panamanians of widely divergent socioeconomic strata were studied during a 15-day tour through mexico. twenty-three (36%) tourists experienced 27 episodes of travelers' diarrhea that were caused by seven different pathogens. the most commonly identified etiologic agents were rotavirus (26%), norwalk virus (15%), and campylobacter fetus (11%), whereas enterotoxigenic escherichia coli was not fre ...19816273474
bactericidal activity of antibiotics against campylobacter fetus subspecies intestinalis. 19816262415
summer diarrhoea in african infants and children.of 70 black south african infants and children with acute summer diarrhoea, 30 (43%) were infected with enteropathogenic serogroups of escherichia coli (epec), 13 (19%) with enterotoxigenic gram-negative bacilli, 12 (17%) with salmonella sp., 6 (9%) with shigella sp., and 3 (4%) with rotaviruses. 13 (19%) patients were infected simultaneously with more than one enteropathogen, and no pathogen was detected in 22 (31%). in addition, 6 (15%) of 41 unselected patients were excreting campylobacter fe ...19806257185
[a case report of septicaemia due to campylobacter fetus variety intestinalis on a cancerous patient (author's transl)].the authors report a case of campylobacter fetus septicaemia from a cancerous patient. the bacterium was isolated from three blood cultures. the infection characteristics are briefly discussed.19806256917
respiratory systems and cytochromes in campylobacter fetus subsp. intestinalis.cell suspensions of campylobacter fetus subsp. intestinalis grown microaerophilically in complex media consumed oxygen in the presence of formate, succinate, and dl-lactate, and membranes had the corresponding dehydrogenase activities. the cells and membranes also had ascorbate-n,n,n',n'-tetramethyl-p-phenylenediamine oxidase activity which was cyanide sensitive. the fumarate reductase activity in the membranes was inhibited by p-chloromercuriphenylsulfonate, and this enzyme was probably respons ...19806254951
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