PMID(sorted ascending)
a case of campylobacter fetus endocarditis of the tricuspid valve unaccompanied by fever. 200111599526
campylobacter fetus uses multiple loci for dna inversion within the 5' conserved regions of sap homologs.campylobacter fetus cells possess multiple promoterless sap homologs, each capable of expressing a surface layer protein (slp) by utilizing a unique promoter present on a 6.2-kb invertible element. each sap homolog includes a 626-bp 5' conserved region (fcr) with 74 bp upstream and 552 bp within the open reading frame. after dna inversion, the splice is seamless because the fcrs are identical. in mutant strain 23d:aca2k101, in which sapa and sapa2 flanking the invertible element in opposite orie ...200111673436
campylobacter fetus pericarditis in a patient with beta-thalassemia: case report and review of the literature.a case of pericardial effusion due to campylobacter fetus in a patient with thalassemia is presented. the patient failed to respond to ceftriaxone and clarithromycin despite in vitro susceptibility, but improved after pericardiectomy and ampicillin. pericarditis due to c. fetus has rarely been reported. a high index of suspicion is essential to recognise this organism, because of its special microbiological characteristics.200111678937
bacterial genetic fingerprint: a reliable factor in the study of the epidemiology of human campylobacter enteritis?the rate of human intestinal infections with more than a single campylobacter strain was determined and the genetic variabilities of campylobacter strains throughout an infection episode were investigated by means of pulsed-field gel electrophoresis (pfge) and enterobacterial repetitive intergenic consensus sequence pcr (eric-pcr). for 48 and 49 of 50 patients, all isolates from one sample showed identical patterns by pfge and eric-pcr, respectively. throughout an infection episode in 47 of 52 p ...200111682547
identification, characterization, and variation in expression of two serologically distinct o-antigen epitopes in lipopolysaccharides of campylobacter fetus serotype a strains.monoclonal antibodies (mabs) to the lipopolysaccharide (lps) o-antigens of campylobacter fetus serotype a and b strains were produced. eight mabs specific for serotype a lps were characterized on immunoblots of c. fetus serotype a lps. two immunoblot patterns were observed and were used to divide the eight mabs into two groups. mabs m1177 and m1194 were selected as representative of the two groups and were used in an enzyme-linked immunosorbent assay (elisa) to examine the lps o-antigen epitopes ...200111705938
an optimised recovery method for thermophilic campylobacter from liver.the past three decades have witnessed the rise of campylobacter enteritis in man from virtual obscurity to notoriety, with present isolation rates superseding those of other enteric pathogens such as salmonella spp. and shigella spp. in most developed countries. although campylobacters are not completely new to applied bacteriology, they have evaded traditional isolation techniques used for the isolation of pure cultures, apart from single isolations that were free from competing organisms. offa ...200111741507
characterization of an autotrophic sulfide-oxidizing marine arcobacter sp. that produces filamentous sulfur.a coastal marine sulfide-oxidizing autotrophic bacterium produces hydrophilic filamentous sulfur as a novel metabolic end product. phylogenetic analysis placed the organism in the genus arcobacter in the epsilon subdivision of the proteobacteria. this motile vibrioid organism can be considered difficult to grow, preferring to grow under microaerophilic conditions in flowing systems in which a sulfide-oxygen gradient has been established. purified cell cultures were maintained by using this appro ...200211772641
evaluation of a novel heminested pcr assay based on the phosphoglucosamine mutase gene for detection of helicobacter pylori in saliva and dental plaque.a novel heminested pcr protocol was developed for the specific detection of helicobacter pylori at low copy numbers. a set of primers specific for the phosphoglucosamine mutase gene (glmm) of h. pylori produced a 765-bp fragment that was used as template for the heminested primer pair delineating a 496-bp fragment. by using agarose gel electrophoresis for detection of the heminested pcr-amplified products, amplification of h. pylori genomic dna was achieved at concentrations as low as 0.1 pg, eq ...200211773117
succinate:quinone oxidoreductases from epsilon-proteobacteria.the epsilon-proteobacteria form a subdivision of the proteobacteria including the genera wolinella, campylobacter, helicobacter, sulfurospirillum, arcobacter and dehalospirillum. the majority of these bacteria are oxidase-positive microaerophiles indicating an electron transport chain with molecular oxygen as terminal electron acceptor. however, numerous members of the epsilon-proteobacteria also grow in the absence of oxygen. the common presence of menaquinone and fumarate reduction activity su ...200211803019
historical origins of current ibd concepts. 200111819757
efficient isolation of campylobacters from stools: what are we missing? 200211896083
campylobacter fetus bacteraemia in a renal graft recipient. 200211917074
development and evaluation of an enzyme-linked immunosorbent assay for quantification of the humoral response of cattle vaccinated against campylobacter develop a reliable elisa by use of a unique antigen preparation for serum igg quantification after vaccination against campylobacter fetus in cattle.200211939324
development and application of a new scheme for typing campylobacter jejuni and campylobacter coli by pcr-based restriction fragment length polymorphism analysis.a molecular typing approach for campylobacter jejuni and campylobacter coli was developed with restriction fragment length polymorphism analysis of a 9.6-kb pcr-amplified portion of the lipopolysaccharide gene cluster. sixty-one penner serotype reference strains were analyzed with this new genotyping scheme, and 32 genogroups were found. eleven additional genogroups were obtained from 87 clinical c. jejuni strains tested. this molecular typing method shows a correlation with the penner heat-stab ...200211980961
thymoma associated with hypogammaglobulinemia (good's syndrome): report of a case.a 63-year-old man was admitted to our hospital for treatment of hypogammaglobulinemia with thymoma (good's syndrome). tests for immunological function showed an abnormality in humoral immunity with decreases in the proportion of cells bearing b-cell markers in the peripheral blood and bone marrow. the patient was found to have campylobacter fetus sepsis caused by the hypogammaglobulinemia due to humoral immunodeficiency, and he was given gamma-globulin supplement. thymectomy was performed due to ...200211991514
effect of chlortetracycline in a trace mineral salt mix on fertility traits in beef cattle females in florida.the study objective was to determine the effect of chlortetracycline in an ad libitum trace-mineralized salt mix given to heifers before and(or) during bull exposure on the proportion pregnant and the time to conception in a fixed breeding period. heifers (n = 768), 13 to 15 mo of age, were individually identified, immunized (leptospira, campylobacter fetus), examined (body condition score, vaginal lesion score, bw), and randomly allocated within 2 x 2 factorial blocks of treatments where trace ...200212002324
detection of campylobacter antibodies in sheep sera by a dot-elisa using acid extracts from c. fetus ssp. fetus and c. jejuni strains and comparison with a complement fixation this study, a dot-enzyme-linked immunosorbent assay (dot-elisa) was evaluated in comparison with a complement fixation test (cft) for the detection of campylobacter antibodies in sheep sera. acid glycine extracts (age) of both campylobacter fetus ssp. fetus and campylobacter jejuni strains that had been isolated from the gall-bladder of slaughtered sheep was used as antigen in both tests. a total of 153 sheep sera from aborted (74) and slaughtered (79) sheep were examined by both dot-elisa an ...200212019946
walkerton, 2 years later: "memory fades very quickly". 200212041857
campylobacter fetus subspecies venerealis surface array protein from bovine isolates in brazil.the electrophoretic patterns of 31 campylobacter fetus subspecies venerealis capsular surface array protein (sap) isolated from bovines in reproduction from different regions of brazil were analyzed. the persistence of the bacteria in the reproductive tract of naturally infected bovines and the dynamic of sap expression were also evaluated. cervical mucous and prepucial aspirates from five animals naturally infected were cultured for isolation of campylobacter fetus and the saps extracted from t ...200212070688
monoclonal antibodies specific for campylobacter fetus lipopolysaccharides.four monoclonal antibodies (mabs) (m1357, m1360, m1823 and m1825) which reacted with campylobacter fetus lipopolysaccharide (lps) core region epitopes were produced and characterized. reactivity of these mabs with c. fetus core lps epitopes was determined by enzyme-linked immunosorbent assay (elisa) with whole cell proteinase k digests and phenol-water extracted lps, and by immunoblotting with proteinase k digests. the specificities of the four mabs were evaluated using an indirect elisa. one of ...200212079745
patterns of sequence conservation in the s-layer proteins and related sequences in clostridium difficile.clostridium difficile is the etiological agent of antibiotic-associated diarrhea. among the factors that may play a role in infection are s-layer proteins (slps). previous work has shown these to consist mainly of two components, resulting from the cleavage of a precursor encoded by the slpa gene. the high-molecular-weight (mw) subunit is related both to amidases from b. subtilis and to at least another 28 gene products in c. difficile strain 630. to gain insight into the functions of the slps a ...200212081960
clostridium difficile genotyping based on slpa variable region in s-layer gene sequence: an alternative to serotyping.recent investigations of clostridium difficile cell wall components have revealed the presence of an s-layer encoded by the slpa gene. the aim of this study was to determine whether slpa genotyping can be used as an alternative to serotyping. the variable regions of slpa were amplified by pcr from serogroup reference strains and various clinical isolates chosen randomly. amplified products were analyzed after restriction enzyme digestion and dna sequencing. the sequences of the variable region o ...200212089261
growth of campylobacter jejuni supported by respiration of fumarate, nitrate, nitrite, trimethylamine-n-oxide, or dimethyl sulfoxide requires oxygen.the human gastrointestinal pathogen campylobacter jejuni is a microaerophilic bacterium with a respiratory metabolism. the genome sequence of c. jejuni strain 11168 reveals the presence of genes that encode terminal reductases that are predicted to allow the use of a wide range of alternative electron acceptors to oxygen, including fumarate, nitrate, nitrite, and n- or s-oxides. all of these reductase activities were present in cells of strain 11168, and the molybdoenzyme encoded by cj0264c was ...200212107136
recurrent septicemia due to campylobacter fetus and campylobacter lari in an immunocompetent patient.we describe a severe and recurrent septicemia due to campylobacter in a 75-year-old immunocompetent patient. two campylobacter strains were detected in several blood cultures. campylobacter fetus and campylobacter lari were identified with pcr tests based on species-specific nucleotide sequences for the 16s rrna gene.200212120946
homologs of the rml enzymes from salmonella enterica are responsible for dtdp-beta-l-rhamnose biosynthesis in the gram-positive thermophile aneurinibacillus thermoaerophilus dsm 10155.the glycan chains of the surface layer (s-layer) glycoprotein from the gram-positive, thermophilic bacterium aneurinibacillus (formerly bacillus) thermoaerophilus strain dsm 10155 are composed of l-rhamnose- and d-glycero-d-manno-heptose-containing disaccharide repeating units which are linked to the s-layer polypeptide via core structures that have variable lengths and novel o-glycosidic linkages. in this work we investigated the enzymes involved in the biosynthesis of thymidine diphospho-l-rha ...200212147463
fluorescent amplified fragment length polymorphism probabilistic database for identification of bacterial isolates from urinary tract infections.the ability of the fluorescent amplified fragment length polymorphism (faflp) technique to identify bacterial isolates from urinary tract infections (utis) was investigated. faflp was carried out using the single primer combination msei plus ct and ecori plus 0, and information-rich faflp profiles were generated from all 69 uti isolates studied, which comprised both gram-negative and gram-positive bacteria encompassing eight genera. the genetic relatedness of these 69 bacteria was determined by ...200212149332
campylobacter-induced fetal death in a rhesus monkey.a pregnant 4-year-old rhesus monkey (macaca mulatta) was presented with a history of acute vaginal bleeding. physical examination revealed an open cervix. an ultrasound scan demonstrated a viable early third-trimester fetus, approximately 16 weeks of gestational age. hematology results showed that the monkey was anemic, with a normal leukogram and döhle bodies. a subsequent cervical culture was positive for campylobacter fetus. the fetus died 3 days later, and a necropsy of the fetus and placent ...200212243477
campylobacter-associated guillain-barré syndrome after orthotopic liver transplantation for hepatitis c cirrhosis: a case report.guillain-barré syndrome is characterized by acute paralysis and ascending neuropathy due to an inflammatory attack on the myelin of peripheral nerves. about 2/3 of patients with guillain-barré syndrome have an infection 1-3 weeks before the onset of the symptoms. guillain-barré syndrome has rarely been reported after solid organ transplantation (18 cases with three cases after liver transplantation), and these cases are mostly related to a cmv infection. we describe a 64-year-old male patient wh ...200212270750
long-term antibody responses following human infection with campylobacter jejuni.epidemiological evidence suggests prior infection of humans by campylobacter jejuni leads to protection against disease following further exposure. it is known that infections elicit strong antibody responses following the onset of disease and that antibody levels are elevated in putatively immune populations. to determine if systemic and mucosal antibodies induced by a confirmed infection remain at elevated levels for prolonged periods, repeat serum, saliva and urine samples were taken from cam ...200212296859
detection of helicobacter colonization of the murine lower bowel by genus-specific pcr-denaturing gradient gel electrophoresis.helicobacter genus-specific pcr and denaturing gradient gel electrophoresis can detect and speciate the helicobacters that colonize the lower bowel of laboratory mice. the method's sensitivity is comparable to that of species-specific pcr and may detect unnamed helicobacter species. this approach should prove useful for commercial and research murine facilities.200212324369
[a case of meningoencephalitis and spondylodiscitis caused by campylobacter fetus subsp. fetus infection].campylobacter fetus subsp. fetus (c. fetus) is a gram-negative, curved, rod-shaped microaerophile, occasionally may cause meningitis or meningoencephalitis in humans. this report documents the case of 49-year-old man with lumbar spondylodiscitis and meningoencephalitis caused by c. fetus infection. on admission, the patient was delirious and severe inflammatory reactions were seen in his serum. cerebrospinal fluid (cfs) revealed normal glucose concentration and moderate mononuclear leukocytosis. ...200212355851
thermo-tolerant campylobacter fetus bacteraemia identified by 16s ribosomal rna gene sequencing: an emerging pathogen in immunocompromised patients.eight campylobacter isolates that were able to grow at 25 degrees c and 42 degrees c and had the same biochemical profile were isolated from the blood of eight immunocompromised patients. conventional biochemical tests were unable to determine whether they were isolates of thermo-tolerant c. fetus, h2s-negative c hyointestinalis, or a new campylobacter species. sequencing of the 16s ribosomal rna genes showed that all eight isolates had the same nucleotide sequence and this was identical to that ...200212358064
structural analysis and evidence for dynamic emergence of bacillus anthracis s-layer networks.surface layers (s-layers), which form the outermost layers of many bacteria and archaea, consist of protein molecules arranged in two-dimensional crystalline arrays. bacillus anthracis, a gram-positive, spore-forming bacterium, responsible for anthrax, synthesizes two abundant surface proteins: sap and ea1. regulatory studies showed that ea1 and sap appear sequentially at the surface of the parental strain. sap and ea1 can form arrays. the structural parameters of s-layers from mutant strains (e ...200212426331
isolation of three new surface layer protein genes (slp) from lactobacillus brevis atcc 14869 and characterization of the change in their expression under aerated and anaerobic conditions.two new surface layer (s-layer) proteins (slpb and slpd) were characterized, and three slp genes (slpb, slpc, and slpd) were isolated, sequenced, and studied for their expression in lactobacillus brevis neotype strain atcc 14869. under different growth conditions, l. brevis strain 14869 was found to form two colony types, smooth (s) and rough (r), and to express the s-layer proteins differently. under aerobic conditions r-colony type cells produced slpb and slpd proteins, whereas under anaerobic ...200212446628
prevalence of campylobacter jejuni, campylobacter lari, and campylobacter coli in different ecological guilds and taxa of migrating birds.a total of 1,794 migrating birds trapped at a coastal site in southern sweden were sampled for detection of campylobacter spp. all isolates phenotypically identified as campylobacter jejuni and a subset of those identified as non-c. jejuni were identified to the species level by pcr-based techniques. c. jejuni was found in 5.0% of the birds, campylobacter lari was found in 5.6%, and campylobacter coli was found in 0.9%. an additional 10.7% of the tested birds were infected with hippurate hydroly ...200212450810
isolation of campylobacter fetus subsp. fetus from a patient with cellulitis.campylobacter fetus subsp. fetus is a gram-negative, slender, spirally curved bacterial pathogen. it has been isolated from human blood, spinal fluid, and abscesses, but cellulitis associated with bacteremia is rare. we report its isolation from a blood culture of a human patient with cellulitis as well as difficulties encountered in determining the identity of the subspecies of c. fetus.200212454199
role of s-layer protein antigenic diversity in the immune responses of sheep experimentally challenged with campylobacter fetus subsp. fetus.surface layer proteins (slps) are essential for induction of abortion by campylobacter fetus subsp. fetus in experimentally challenged ewes. these proteins are encoded by multiple sap genes and vary in size and antigenicity. the role of slp antigenic variation during experimental ovine infection was investigated. following subcutaneous challenge, the slps were highly antigenic, and antibodies were detected in serum, milk, bile, and urine. fecal anti-slp antibodies were detected only in animals c ...200312496160
cd4+cd25+ t(r) cells suppress innate immune pathology through cytokine-dependent mechanisms.cd4(+)cd25(+) regulatory t (t(r)) cells can inhibit a variety of autoimmune and inflammatory diseases, but the precise mechanisms by which they suppress immune responses in vivo remain unresolved. here, we have used helicobacter hepaticus infection of t cell-reconstituted recombination-activating gene (rag)(-/-) mice as a model to study the ability of cd4(+)cd25(+) t(r) cells to inhibit bacterially triggered intestinal inflammation. h. hepaticus infection elicited both t cell-mediated and t cell ...200312515818
comparison of recovery of blood culture isolates from two bact/alert fan aerobic blood culture bottles with recovery from one fan aerobic bottle and one fan anaerobic bottle.traditionally, a routine blood culture for adult patients consisted of paired aerobic and anaerobic bottles, but the routine use of an anaerobic blood culture bottle has been challenged in recent years. in this study, we compared the recovery of two fan aerobic bottles with one fan aerobic and one fan anaerobic bottle. each pair of bottles was collected by a separate collection procedure, and each bottle held a recommended 8- to 12-ml draw. a total of 704 clinically significant isolates were rec ...200312517851
rapid detection of methicillin-resistant staphylococcus aureus directly from sterile or nonsterile clinical samples by a new molecular assay.a rapid procedure was developed for detection and identification of methicillin-resistant staphylococcus aureus (mrsa) directly from sterile sites or mixed flora samples (e.g., nose or inguinal swabs). after a rapid conditioning of samples, the method consists of two main steps: (i) immunomagnetic enrichment in s. aureus and (ii) amplification-detection profile on dna extracts using multiplex quantitative pcr (5'-exonuclease qpcr, taqman). the triplex qpcr assay measures simultaneously the follo ...200312517857
evaluation of 11 pcr assays for species-level identification of campylobacter jejuni and campylobacter coli.we examined the sensitivity and specificity of 11 pcr assays described for the species identification of campylobacter jejuni and campylobacter coli by using 111 type, reference, and field strains of c. jejuni, c. coli, and campylobacter lari. for six assays, an additional 21 type strains representing related campylobacter, arcobacter, and helicobacter species were also included. pcr tests were initially established in the laboratory by optimizing conditions with respect to five type and referen ...200312517869
epidemiology and antimicrobial susceptibilities of 111 campylobacter fetus subsp. fetus strains isolated in québec, canada, from 1983 to 2000.the epidemiology and antimicrobial susceptibilities of 111 campylobacter fetus subsp. fetus strains isolated from 103 patients from 1983 to 2000 in québec, canada, were determined. the median number of patients infected annually with this bacteria was seven, with an incidence of 0.1 per 100,000 population. the male-to-female ratio was 1.1 to 1.0. the patients originated from 13 of the 18 québec socioeconomic regions. the age range of the patients was 6 months to 90 years old, 53% being > or = 70 ...200312517895
campylobacter fetus adheres to and enters int 407 cells.campylobacter fetus is a gram-negative bacterial pathogen of humans and ungulates and is normally transmitted via ingestion of contaminated food or water with infection resulting in mild to severe enteritis. however, despite clinical evidence that c. fetus infection often involves transient bacteremic states from which systemic infection may develop and the frequent isolation of c. fetus from extra-intestinal sites, this organism displays very poor invasiveness in in vitro models of infection. i ...200212556127
pseudobacteraemia in a patient with neutropenic fever caused by a novel paenibacillus species: paenibacillus hongkongensis sp. characterise a strain of gram negative aerobic straight or slightly curved rods (hku3) isolated from the blood culture of a 9 year old chinese boy with neutropenic fever and pseudobacteraemia.200312560460
specific detection of arcobacter and campylobacter strains in water and sewage by pcr and fluorescent in situ hybridization.the aim of this study was to evaluate pcr and fluorescent in situ hybridization (fish) techniques for detecting arcobacter and campylobacter strains in river water and wastewater samples. both 16s and 23s rrna sequence data were used to design specific primers and oligonucleotide probes for pcr and fish analyses, respectively. in order to assess the suitability of the methods, the assays were performed on naturally and artificially contaminated samples and compared with the isolation of cells on ...200312571045
pcr-restriction fragment length polymorphism analysis for detection of point mutations associated with macrolide resistance in campylobacter spp.a 23s rrna gene fragment in domain v was sequenced from 30 clinical isolates of campylobacter spp., including 22 resistant to macrolides. two point mutations associated with erythromycin resistance were identified at positions 2074 and 2075 on the 23s rrna gene (homologous to a2142c and a2143g mutations in helicobacter pylori) in which an adenine residue is also replaced with a cytosine and a guanine residue, respectively. a combined pcr-restriction fragment length polymorphism technique was dev ...200312604552
a real-time pcr assay for the detection of campylobacter jejuni in foods after enrichment culture.a real-time pcr assay was developed for the quantitative detection of campylobacter jejuni in foods after enrichment culture. the specificity of the assay for c. jejuni was demonstrated with a diverse range of campylobacter species, related organisms, and unrelated genera. the assay had a linear range of quantification over six orders of magnitude, and the limit of detection was approximately 12 genome equivalents. the assay was used to detect c. jejuni in both naturally and artificially contami ...200312620820
phenotypic and molecular characterization of bovine campylobacter fetus strains isolated in brazil.the objective of the present study was to characterize the phenotypic and molecular aspects of campylobacter fetus strains isolated from bovine herds with reproductive problems. thirty-one brazilian field isolates, together with one reference strain of each subspecies of c. fetus, were analyzed. the strains were submitted to phenotypic identification followed by subspecies characterization using the polymerase chain reaction (pcr) and numeric evaluation of restriction fragment length polymorphis ...200312637000
[a man with campylobacter endocarditis, treatable as campylobacter fetus following identification].a 48-year-old dutch patient presented with general malaise, recurrent fever and weight loss. routine cultures identified a campylobacter in two blood cultures. when initial treatment with clarithromycin failed, the patient developed endocarditis. dna sequencing identified campylobacter fetus subspecies fetus (c. fetus). the patient recovered after treatment of the infection with imipenem and gentamycin, based on the minimal inhibiting concentration. c. fetus is a rare cause of infection in human ...200312661460
[identification and differentiation of campylobacter fetus subspecies by pcr].the species campylobacter (c.) fetus is divided into the subspecies venerealis and fetus, which differ in epidemiology and clinical importance. the differences between these subspecies make an accurate distinction essential. differentiation of c. fetus by traditional microbiological methods is only based on two reactions (tolerance to glycin, na selenite reduction), in which c. fetus ssp. venerealis reacts negatively. however, the value of both reactions is limited. we used a specific pcr-based ...200312666499
effect of two commercial vaccines to campylobacter fetus subspecies on heifers naturally challenged.efficacy of two commercial vaccines containing campylobacter fetus subspecies on heifers naturally challenged by service with an infected bull was tested. sixteen heifers were vaccinated parentally two times with 3 weeks as interval, eight with commercial vaccine a and the other eight with commercial vaccine b. eight other heifers were used as unvaccinated controls. forty days after the first vaccine dose, the heifers were served by an infected bull during 60 days. measure of systemic immune res ...200312675898
effect of transport enrichment medium, transport time, and growth medium on the detection of campylobacter fetus subsp. venerealis.the combination of medium and growth conditions, including transport enrichment medium (tem), transport time, tem incubation time, and growth medium, that best support campylobacter fetus subsp. venerealis while inhibiting contaminants was studied. the 3 tems evaluated, weybridge, cary-blair, and 0.85% saline solution, were inoculated with preputial smegma spiked with c. fetus subsp. venerealis and held in the laboratory for 4 or 24 hours before inoculation onto growth medium. the effect of over ...200212680641
structure and genotypic plasticity of the campylobacter fetus sap locus.the campylobacter fetus surface layer proteins (slps), encoded by five to nine sapa homologues, are major virulence factors. to characterize the sapa homologues further, a 65.9 kb c. fetus genomic region encompassing the sap locus from wild-type strain 23d was completely sequenced and analysed; 44 predicted open reading frames (orfs) were recognized. the 53.8 kb sap locus contained eight complete and one partial sapa homologues, varying from 2769 to 3879 bp, sharing conserved 553-2622 bp 5' regi ...200312694614
failure of surface ring mutant strains of helicobacter mustelae to persistently infect the ferret stomach.helicobacter mustelae, the gastric pathogen of ferrets, produces an array of surface ring structures which have not been described for any other member of the genus helicobacter, including h. pylori. the unique ring structures are composed of a protein named hsr. to investigate whether the hsr rings are important for colonization of the ferret stomach, ferrets specific pathogen free for h. mustelae were inoculated with an hsr-deficient mutant strain or the wild-type h. mustelae strain. quantitat ...200312704104
presumptive toxoplasma gondii abortion in a sheep.a primiparous ewe aborted in mid-gestation. toxoplasma gondii was suspected as the cause of abortion and a presumptive diagnosis of t. gondii abortion was based on histological lesions of the placenta.200312715986
usefulness of the microseq 500 16s ribosomal dna-based bacterial identification system for identification of clinically significant bacterial isolates with ambiguous biochemical profiles.due to the inadequate automation in the amplification and sequencing procedures, the use of 16s rrna gene sequence-based methods in clinical microbiology laboratories is largely limited to identification of strains that are difficult to identify by phenotypic methods. in this study, using conventional full-sequence 16s rrna gene sequencing as the "gold standard," we evaluated the usefulness of the microseq 500 16s ribosomal dna (rdna)-based bacterial identification system, which involves amplifi ...200312734240
humoral immune responses to s-layer-like proteins of bacteroides forsythus.bacteroides forsythus is one of the important periodontopathic bacteria, and this microorganism is known to have an s-layer outside the outer membrane. the s-layer-like antigens were recently isolated from b. forsythus, and they were found to be 270- and 230-kda proteins in the envelope fraction. in this study, these proteins were confirmed to be specific to b. forsythus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and they were clearly recognized by sera from patients with adul ...200312738635
phenotypic and genotypic differentiation of campylobacter spp. isolated from austrian broiler farms: a identification scheme based on restriction fragment length polymorphism of polymerase chain reaction products (pcr-rflp) was developed to differentiate isolates of the genera campylobacter, arcobacter and helicobacter. based on the 16s rrna gene of these genera, pcr amplified a 1216-bp fragment. the amplicons were digested with the restriction enzymes rsai and ecorv. additional differentiation was obtained using a pcr-assay based on the hippuricase gene. genotyping was performed on several re ...200312745378
campylobacter fetus bacteremia in a patient with adult t cell leukemia. 200312766848
sandwich hybridisation assay for quantitative detection of yeast rnas in crude cell lysates.background: a rapid microtiter plate based sandwich hybridization assay was developed for detection and quantification of single rna species using magnetic beads. following solution hybridization target rna molecules were collected by biotin-streptavidin affinity binding and detected by fluorescence signal generated by alkaline phosphatase. the 18s rrna and suc2 mrna of saccharomyces cerevisiae were used as model rna target molecules. results: the sensitivity of the assay was approximately 1.2 x ...200312780940
use of pcr for direct detection of campylobacter species in bovine feces.this study reports on the use of pcr to directly detect and distinguish campylobacter species in bovine feces without enrichment. inhibitors present in feces are a major obstacle to using pcr to detect microorganisms. the qiaamp dna stool minikit was found to be an efficacious extraction method, as determined by the positive amplification of internal control dna added to bovine feces before extraction. with nested or seminested multiplex pcr, campylobacter coli, c. fetus, c. hyointestinalis, and ...200312788747
[a man with campylobacter endocarditis, treatable as campylobacter fetus following identification]. 200312892017
rapid detection of campylobacter jejuni in chicken rinse water by melting-peak analysis of amplicons in real-time polymerase chain reaction.five dna extraction protocols for the detection of campylobacter spp. by polymerase chain reaction (pcr) were compared. a method involving triton x-100 produced template dna of sufficient quality to allow the detection of campylobacter jejuni at levels of 100 cfu/ml in pure culture. primers were designed on the basis of the cadf gene sequence. with a sybr green i real-time pcr assay, these primers amplified only sequences present in c. jejuni to produce a product with a melting temperature of 81 ...200312929818
toward an international standard for pcr-based detection of food-borne thermotolerant campylobacters: validation in a multicenter collaborative part of a european research project, the performance of a pcr assay to detect food-borne thermotolerant campylobacters (campylobacter jejuni, c. coli, and c. lari) was evaluated through an international collaborative trial involving 12 participating laboratories. dna from 10 target and 8 nontarget strains was tested, and the results were reported as the presence of a positive signal after gel electrophoresis. the overall inclusivity (sensitivity) was 93.7%, and the exclusivity (specificity) w ...200312957959
suitable thioglycolate media for the cultivation of vibrio fetus. 195212999711
[immunochemical researches on vibrio fetus. i. preliminary serological study of 10 strains of different origins]. 195213017155
[immunochemical researches on vibrio fetus. ii. chemical fractionation of microbes and serological study of two antigenic fractions]. 195213017156
a study of the pathology of embryonating chicken eggs inoculated with vibrio fetus. 195313031019
preparations of antigen for detection of agglutinins for vibrio fetus. 195313065650
serological relationships of twenty three ovine and three bovine strains of vibrio fetus. 195313065651
experimental vibrio fetus infection in guinea pigs. i. bacteriological aspects. 195313065652
a satisfactory method for the culture of vibrio fetus. 195313067636
an electron micrograph of vibrio fetus. 195313094936
the tampon method of collection and the examination of cervicovaginal mucus for vibrio fetus agglutinins. 195313117727
experimental vibrio fetus infection in guinea pigs. ii. pathological aspects. 195413124691
transmission of experimental vibrio fetus infection among guinea pigs and hamsters. 195413148490
[septicemia due to vibrio fetus in a gastrectomized patient with blood protein deficiency]. 195413198378
experimental vibrio fetus infection in guinea pigs. iii. pathogenicity studies and the effect of guinea pig passage upon agglutinogens. 195413207584
the illustration of the morphology of vibrio fetus by electron microscopy. 195413207590
[vibrio fetus septicemia in a man]. 195413209343
the growth and viability of vibrio fetus and related vibrios in media containing ox bile. 195513219933
a preliminary report on the identification of vibrio fetus. 195513228871
serological and physiological relationships between strains of vibrio fetus. 195513228888
experimental vibrio fetus infection in male hamsters. 195513228893
the stimulated growth of vibrio fetus by the use of hormones. 195513251994
reproductive efficiency and serological findings in heifers experimentally infected with vibrio fetus. 195513259022
serological variants among strains of vibrio fetus. 195613277332
gaseous requirements for growth of vibrio fetus. 195613283241
physiological characteristics differentiating vibrio fetus and other vibrios. 195613302664
in vitro and in vivo use of antibiotics against vibrio fetus. 195613319177
[fundamentals of serological investigation of vibrio fetus infection of french cattle]. 195613327504
[elements of bacteriological research on the infection of french cattle by vibrio fetus]. 195613327522
[septicemia with endocarditis caused by vibrio fetus]. 195613342961
serological responses of cattle to vibrio fetus vaccine as measured by the complement-fixation test and tube-agglutination test. 195613362772
colonial and antigenic variations of vibrio fetus. 195613362799
[the diversity of pathological manifestations during infection by vibrio fetus in various species]. 195613364484
effect of gaseous environment on growth and catalase content of vibrio fetus cultures of bovine origin. 195613366935
[new culture method for the isolation of vibrio fetus from genital secretions of male and female cattle]. 195513371832
[a method of determining a vibrio fetus by polymicrobial samples particularly from the preputial fluid; selective heart-blood-gelose medium with brilliant green agar in microaerobiosis]. 195613375030
human vibriosis caused by vibrio fetus. 195713385129
Displaying items 1101 - 1200 of 1841