TitleAbstractYear(sorted ascending)
revision of campylobacter, helicobacter, and wolinella taxonomy: emendation of generic descriptions and proposal of arcobacter gen. nov.hybridization experiments were carried out between dnas from more than 70 strains of campylobacter spp. and related taxa and either 3h-labeled 23s rrnas from reference strains belonging to campylobacter fetus, campylobacter concisus, campylobacter sputorum, campylobacter coli, and campylobacter nitrofigilis, an unnamed campylobacter sp. strain, and a wolinella succinogenes strain or 3h- or 14c-labeled 23s rrnas from 13 gram-negative reference strains. an immunotyping analysis of 130 antigens ver ...19911704793
oligodeoxynucleotide probes for campylobacter fetus and campylobacter hyointestinalis based on 16s rrna sequences.deoxyoligonucleotide probes were constructed for the identification of campylobacter fetus and campylobacter hyointestinalis based on 16s rrna sequence data. probes were targeted to hypervariable regions of 16s rrna. specificity of oligonucleotide probes was tested in a colony blot assay with type strains of 15 campylobacter and arcobacter species as well as in a slot blot format using genomic dna extracted from field strains of c. fetus and c. hyointestinalis. two oligonucleotides were construc ...19911723076
characterization of an immunoreactive species-specific 19-kilodalton outer membrane protein from helicobacter pylori by using a monoclonal antibody.immunoblotting experiments on hyperimmune rabbit serum and sera from patients with helicobacter pylori gastritis showed a consistent antibody response to a 19-kda outer membrane protein antigen. a monoclonal antibody, designated hp 40, which reacted by western immunoblotting with this protein was produced. it was shared by all h. pylori strains tested (d 273, nctc 11637, and 24 wild strains) but not by the thermophilic campylobacter species, campylobacter fetus, helicobacter mustellae, or helico ...19911761682
campylobacter fetus subspecies fetus infection.during a six-year period five patients with campylobacter fetus subspecies fetus infections were seen at the mayo clinic in rochester, minnesota. bacteremia was observed in two patients, one presenting with aortic valve endocarditis and the other with abdominal atherosclerotic aortic aneurysm. c. fetus subsp. fetus was isolated from tibial tissue of a patient with osteomyelitis. diarrhea was the main complaint of two further patients, and was also mentioned by the patient with the aortic aneurys ...19911762387
prevalence of bovine campylobacteriosis in indigenous cattle of three states in nigeria.a survey of bovine campylobacteriosis in breeding bulls and cows was carried out in the states of kaduna, kano and borno. six hundred and eighty nine cattle composed of 585 and 104 breeding bulls and cows respectively were sampled. campylobacter fetus subsp. venerealis was isolated from 12 bulls while campylobacter fetus subsp. fetus was isolated from three of them. campylobacter fetus subsp. fetus was isolated from four cows while campylobacter fetus subsp. veneralis was isolated from one cow. ...19911763437
comparison of surface antigens of some campylobacter fetus subsp. fetus strains of ovine origin by polyacrylamide gel electrophoresis and immunoblotting.sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and immunoblotting were used to identify and to compare the surface antigens of eight c. fetus subsp. fetus strains. seven strains (one of serogroup a and six of serogroup b) were isolated from aborted ovine fetuses, while one strain (serogroup a) originated from an aborted calf fetus. saline extracts at 56 degrees c and 100 degrees c were used as antigens. antisera were produced in rabbits. in saline extracts (56 degrees c) of ...19911776377
campylobacter spondylodiscitis and deficiency of cellular immunity.we describe a case of spondylodiscitis caused by campylobacter fetus in a previously healthy patient. this is the first report of this agent causing discovertebral infection, which generated cellular immune deficiency, reversible with the cure.19911795335
[bacteremia from campylobacter fetus. increasing interest and incidence].three bacteremias of campylobacter fetus were described. one was a male patient and two were females. underlying illnesses were present in all of them (hodgkin disease, aids and hepatic cirrhosis respectively). they were all admitted because of fever and no other symptoms of infectious focus were present. physical findings were not relevant. the blood cultures became positive in days 6, 7 and 9 respectively. antibiotic treatments were not standardised, so no conclusions can be drawn. the evoluti ...19911943296
diagnosis by elisa of bovine abortion due to campylobacter enzyme-linked immunosorbent assay (elisa) was developed to detect antigen-specific secretory iga antibody in bovine vaginal mucus after abortion due to campylobacter fetus subsp venerealis. abortions were diagnosed by isolating the organism from 8 foetuses and/or foetal membranes and by histopathology. vaginal mucus was collected from 7 cows shortly after abortion. all showed a high level of iga antibody in their vaginal mucus when they were compared with an uninfected control group. the new ...19911953551
multivariate analyses of cellular fatty acids in bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp.the genera bacteroides, wolinella, and campylobacter contain several similar species that require taxonomic revision. fatty acid profiles of whole bacterial cells have proven useful for taxonomy. in this study, cellular fatty acids from bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp. were identified and quantitated by gas chromatography and gas chromatography-mass spectrometry, and the data were subjected to principal component analyses. bacteroides fragilis, the type s ...19911993755
freeze-substitution of gram-negative eubacteria: general cell morphology and envelope profiles.freeze-substitution was performed on strains of escherichia coli, pasteurella multocida, campylobacter fetus, vibrio cholerae, pseudomonas aeruginosa, pseudomonas putida, aeromonas salmonicida, proteus mirabilis, haemophilus pleuropneumoniae, caulobacter crescentus, and leptothrix discophora with a substitution medium composed of 2% osmium tetroxide and 2% uranyl acetate in anhydrous acetone. a thick periplasmic gel ranging from 10.6 to 14.3 nm in width was displayed in e. coli k-12, k30, and hi ...19911999383
[campylobacter fetus ssp fetus septicemia associated with a colonic abscess].campylobacter is considered to be an opportunistic agent. the authors relate an unusual case with campylobacter fetus ssp fetus (cf) septicemia and colic abscess. human campylobacteriosis is presumed to be a food-born disease related to contaminated animal products such as milk or meat. in some cases cf may be transmitted by drinking water or by fecal soiling via the hands. conventional treatment uses macrolides with decrease the duration of diarrhea and reduce the fecal excretion of cf. macroli ...19912029182
correlation between molecular size of the surface array protein and morphology and antigenicity of the campylobacter fetus s layer.the correlation between the molecular size of the surface layer protein (s protein) and both structure and antigenicity of the campylobacter fetus surface layer (s layer) was investigated in several clinical strains and their spontaneous variants which produce s proteins of molecular weights (mw) different from those of the parents. only three molecular sizes of the s proteins were observed (98, 127, and 149 kda) in the parental and variant strains. immunologically, the 98-kda protein and the 14 ...19912037362
[endocarditis caused by campylobacter fetus]. 19912051801
reattachment of surface array proteins to campylobacter fetus cells.campylobacter fetus strains may be of serotype a or b, a property associated with lipopolysaccharide (lps) structure. wild-type c. fetus strains contain surface array proteins (s-layer proteins) that may be extracted in water and that are critical for virulence. to explore the relationship of s-layer proteins to other surface components, we reattached s-layer proteins onto s- template cells generated by spontaneous mutation or by serial extractions of s+ cells with water. reattachment occurred i ...19921735716
[evaluation of antimicrobial drugs and atmospheres for the isolation of campylobacter fetus subspp. from the bovine genital tract].growth of 3 reference bovine c. fetus strains in media with and without antibiotics and bacteriostats active against the most common contaminant bacteria in the bovine genital tract was evaluated. in addition, 2 regional bovine c. fetus strains and 1 reference c. sputorum biovar bubulus strain were used in some experiments. reference strain c. fetus subsp. venerealis was completely inhibited by polymyxin (> or = 0.25 iu/ml) whereas the other c. fetus strains were not inhibited. in shepler's medi ...19921302865
[neonatal campylobacter fetus septicemia: a case secondary to maternal-fetal contamination].a favourable outcome of a severe cambylobacter fetus sepsis in a neonate is reported. the transmission was probably vertical. his mother experienced diarrhoea 15 days before birth. no infecting organism was isolated from maternal stools, but maternal antibody response was significant. such a perinatal infection with meningitis and septicemia is uncommon. bacteriological diagnosis requires an enriched medium and a long incubation. the diagnosis should be evoked in view of an anamnestic context, a ...19921319039
differentiation of the subspecies of campylobacter fetus by genomic sizing.campylobacter fetus subsp. fetus and c. fetus subsp. venerealis are currently differentiated by tolerance to glycine and by their epidemiology. analysis of c. fetus dna by pulsed-field gel electrophoresis, after digestion with the restriction endonucleases smai and sali, was used to differentiate between the subspecies. all strains presently identified as c. fetus subsp. fetus had a genomic size of 1.1 mb, whereas the majority of the c. fetus subsp. venerealis strains had a genomic size of 1.3 m ...19921354478
nucleotide sequence analysis of the gene encoding the caulobacter crescentus paracrystalline surface layer protein.the entire nucleotide sequence of the rsaa gene, encoding the paracrystalline surface (s) layer protein (rsaa) of caulobacter crescentus cb15a, was determined. the rsaa gene encoded a protein of 1026 amino acids, with a predicted molecular weight of 98,132. protease cleavage of mature rsaa protein and amino acid sequencing of retrievable peptides yielded two peptides: one aligned with a region approximately two-thirds the way into the predicted amino acid sequence and the second peptide correspo ...19921393820
specific detection of campylobacter jejuni and campylobacter coli by using polymerase chain reaction.development of a routine detection assay for campylobacter jejuni and campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (pcr). an oligonucleotide primer pair from a conserved 5' region of the flaa gene of c. coli vc167 was used to amplify a 450-bp region by pcr. the primer pair specifically detected 4 strains of c. coli and 47 strains of c. jejuni; but it did not detect strains of campylobacter fetus, campylobacter lari, campylobacter upsaliensis, cam ...19921400961
nosocomial meningitis due to campylobacter fetus subspecies fetus in a neonatal intensive care unit.we experienced a nosocomial outbreak of campylobacter fetus subspecies fetus (c. fetus) meningitis in a neonatal intensive care unit. a cluster of three infants developed meningitis approximately 1-1.5 months after the index patient was admitted. two asymptomatic intestinal carriers of c. fetus were detected during the outbreak. our experience indicates that c. fetus can cause nosocomial meningitis in neonates, and asymptomatic carriers may play a role in the transmission of the organism.19921442025
anti-enterobacteria antibodies in psoriatic arthritis.the occurrence of certain antibacterial antibodies was studied in the sera of 22 healthy donors (hd) and 66 patients with different diseases. the cases investigated included 22 rheumatoid arthritis (ra), 22 non-arthritic-psoriasis (nap), and 22 psoriatic arthritis (pa) patients. a complement fixation test was used with yersinia enterocolitica 0:3 type (yec), yersinia pseudotuberculosis (ypt), campylobacter jejuni (cj), and campylobacter fetus (cf) antigens; the detection of anti-chlamydia tracho ...19921458698
structural studies of peptidoglycans in campylobacter species.peptidoglycans (pg) from campylobacter coli, campylobacter jejuni, and campylobacter fetus were composed of muramic acid, glucosamine, alanine, glutamic acid, and diaminopimelic acid in a molar ratio of 1.1:1:1.7:1.1:09. thirty percent of the amino groups of diaminopimelic acid were involved in cross-linkages between peptides. during cultivation, c. coli and c. jejuni changed from a spiral to a coccoid form. in c. coli, we could isolate pg only from the spiral forms in yields of 0.8-1.2% by dry ...19921461153
clinical evaluation of the efficacy of inoculating cattle with a vaccine containing tritrichomonas test the efficacy of a polyvalent tritrichomonas foetus vaccine, 130 nulliparous heifers were randomly assigned to either receive the test t foetus vaccine or to serve as nonvaccinated controls. the polyvalent test vaccine consisted of a campylobacter fetus/leptospira canicola-grippotyphosa-hardjo-icterohaemorrhagiae-pamona bacterine containing 5 x 10(7) killed t foetus/dose. the polyvalent control vaccine consisted of the aforementioned formulation without t foetus. heifers were administered ...19921466496
pyogenic arthritis of the hip due to campylobacter fetus--a case report.septic arthritis of the hip caused by campylobacter fetus subsp. fetusis very rare. the authoris isolated c. fetus subsp. fetus from a specimen of the left hip. the patient was a 53-year old man with a history of heavy drinking, diabetes, and chronic hepatitis, and had been suffering from avascular necrosis of both femoral heads. it was considered that the organism invaded already damaged tissue of the joint. the patient was treated with intravenous antibiotics and later received successful tota ...19921502834
[human campylobacteriosis caused by campylobacter fetus]. 19921507827
[acute aortic insufficiency following endocarditis due to infection with campylobacter fetus subspecies fetus].a 50-year-old male alcoholic addict, examined because of diarrhoea with fever was found to have campylobacter jejuni in blood and stool cultures. after administration of broad-spectrum penicillin all acute symptoms disappeared but he lost 8 kg within 3 months and his general state health gradually deteriorated. after 3 months he suddenly developed leg oedema, dyspnoea and bouts of fever up to 38.8 degrees c. a loud cardiac murmur was now heard. echocardiography demonstrated vegetations on the re ...19921516529
characterization of the campylobacter fetus sapa promoter: evidence that the sapa promoter is deleted in spontaneous mutant strains.wild-type campylobacter fetus cells possess s-layer proteins (s+ phenotype), whereas after laboratory passage, spontaneous stable mutants that do not express these proteins (s- phenotype) arise. to determine the molecular mechanisms by which c. fetus changes to the s- phenotype, we studied wild-type strain 23d, from which the sapa gene encoding the 97-kda s-layer protein has been cloned, and strain 23b, a spontaneous s- mutant. we compared these strains with another pair of strains, lp (s+) and ...19921522068
neonatal campylobacter fetus meningitis: a report of an unusual case.campylobacter fetus is a rare cause of meningitis in the pediatric age group and, in particular, among neonates. the clinical presentation of campylobacter meningitis in high-risk neonates is not well-described. a review of campylobacter meningitis by lee et al in 1985 reported nine cases occurring in neonates, of which only one case was caused by c. fetus. in the ensuing six years only three more confirmed cases of neonatal meningitis caused by c. fetus have been reported. we here report anothe ...19921563204
[campylobacter fetus spondylodiscitis].the case of a sixty-two year old man with campylobacter fetus spondylodiscitis is reported. despite of adequate antibiotherapy, the outcome was bad and death occurred. three other cases of campylobacter fetus spondylodiscitis are reported in the literature. tomodensitometry disclosed a paravertebral abscess with gas, that make our observation original.19921579850
isolation and immunogenicity of campylobacter fetus subsp. fetus from an abdominal aortic aneurysm.a campylobacter fetus strain was isolated from an abdominal aortic aneurysm in a 56-year-old man who had no significant chronic illness except arteriosclerosis. the strain was identified on the basis of the usual taxonomic markers and fatty acid profiles. the patient's immunological status regarding campylobacter fetus was studied by western blot. the strong immune response observed against the 99 kda protein of the strain confirms that the s-layer protein is the immunodominant antigen of campyl ...19938112355
shift in s-layer protein expression responsible for antigenic variation in campylobacter fetus.campylobacter fetus strains possess regular paracrystalline surface layers (s-layers) composed of high-molecular-weight proteins and can change the size and crystalline structure of the predominant protein expressed. polyclonal antisera demonstrate antigenic cross-reactivity among these proteins but suggest differences in epitopes. monoclonal antibodies to the 97-kda s-layer protein of campylobacter fetus subsp. fetus strain 82-40lp showed three different reactivities. monoclonal antibody 1d1 re ...19937688715
the association between antibody titres against campylobacter fetus and reproductive efficiency in dairy cattle.the relationship between the antibody titres against campylobacter fetus and various indices of reproductive efficiency was studied in a cross-sectional study of 178 dairy cows from three california dairy herd improvement association herds. blood samples were collected from the lactating cows during december 1986. enzyme-linked immunosorbent assays were used to determine the antibody titres of the cow against campylobacter fetus, haemophilus somnus and leptospira hardjo and were classified as ei ...19938212528
application of enzyme-linked immunosorbent assay for epidemiological studies of diseases of livestock in the tropics of mexico.this study was conducted at the centre for research, teaching and extension in tropical livestock (centro de investigación, enseñanza y extensión en ganadería tropical) of the faculty of veterinary medicine of the national autonomous university of mexico. during the latter part of 1986 and throughout 1988 and 1989, the herd of holstein x zebu cattle at the university was tested for igg antibodies to twenty-one viral, bacterial, rickettsial and parasitic agents. antigens prepared from twenty infe ...19938219327
granulomatous enteritis and campylobacter bacteremia in a horse.a 10-month-old standard-bred colt was examined because of weight loss, fever, and extensive subcutaneous edema of the ventral aspects of its trunk. diagnosis of granulomatous enteritis was based on d-xylose malabsorption, hypoproteinemia, and rectal mucosal biopsy. campylobacter fetus subsp fetus was cultured from jugular venous blood when the horse was admitted to the hospital and 24 hours later. appropriate antimicrobial agents and glucocorticoids failed to effect clinical remission. after eut ...19938226250
role of the s-layer proteins of campylobacter fetus in serum-resistance and antigenic variation: a model of bacterial pathogenesis.campylobacter fetus are microaerophilic gram-negative bacteria that are pathogens of animals and humans. these organisms possess paracrystalline surface (s-) layers, composed of acidic high molecular weight proteins. c. fetus strains possessing s-layers are resistant to c3b binding, which explains both serum and phagocytosis-resistance. c. fetus strains also can vary the subunit protein size, crystalline structure, and antigenicity of the s-layer it expresses. therefore, its s-layer permits c. f ...19938238090
failure of therapeutic vaccination of a bull infected with campylobacter fetus. 19938257318
the association between antibody titres against campylobacter fetus and milk production efficiency in dairy cattle.the association between serological evidence of exposure to campylobacter fetus and milk production performance was studied in 178 lactating cows from three california dairy herd improvement association herds using a cross-sectional study design in december 1986. elisas were used to determine the antibody titres against campylobacter fetus, haemophilus somnus and leptospira hardjo, which were classified as either negative or positive. the status of a cow as negative or positive against c. fetus ...19938284894
campylobacter fetus subspecies fetus peritonitis in continuous ambulatory peritoneal dialysis. 19938290011
septic abortion associated with campylobacter fetus subspecies fetus infection: case report and review of the contrast to the situation in cattle, goat and sheep, campylobacter fetus subspecies fetus only rarely causes disease in humans. while a major inducer of septic abortion in animals, only a minority of clinical infections in humans are found during pregnancy. eleven cases have so far been described in pregnant women. clinical symptomatology is usually mild during gestation but often leads to premature labor. here we present a multigravida with positive cultures for c. fetus who went into septic ...19938300253
prosthetic hip joint infection due to campylobacter fetus.a case of postoperative prosthetic hip joint infection due to campylobacter fetus subsp. fetus is described. difficulties in isolation and antimicrobial susceptibility testing of this organism are discussed.19938308129
rearrangement of sapa homologs with conserved and variable regions in campylobacter fetus.the campylobacter fetus surface-layer (s-layer) proteins mediate both complement resistance and antigenic variation in mammalian hosts. wild-type strain 23d possesses the sapa gene, which encodes a 97-kda s-layer protein, and several sapa homologs are present in both wild-type and mutant strains. here we report that a cloned silent gene (sapa1) in c. fetus can express a functional full-length s-layer protein in escherichia coli. analysis of sapa and sapa1 and partial analysis of sapa2 indicate t ...19938346244
production of cytolethal distending toxin (cldt) by campylobacter fetus subsp. fetus isolated from calves.cytolethal distending toxin (cldt) production by campylobacter fetus subsp. fetus isolated from calves was examined using cho cells. twenty-five of the 26 strains tested were positive for cldt with titer of ranging 1:8 to 1:2,048. cldt positive strains were divided into low and high cytotoxin titer groups. isolates from diarrhea cases tended to produce a significant amount of cldt compared with isolates from liver. cldt produced by c. fetus showed no effect on y-1 cells and was heat-labile and t ...19938357933
[septic campylobacter fetus thrombophlebitis: a new case].the diagnosis of septic thrombophlebitis is difficult and often delayed, but it must be borne in mind in all cases of venous thrombosis accompanied by signs of local and/or systemic infection, or deteriorating under heparin therapy. we report a case of septic thrombophlebitis caused by campylobacter fetus subspecies fetus. the characteristic features, obtained from the literature, of septic thrombophlebitis caused by campylobacter spp are presented.19938362108
helicobacter acinonyx sp. nov., isolated from cheetahs with gastritis.four strains of a novel helicobacter species were isolated from the stomachs of cheetahs (acinonyx jubilatus) with gastritis. these isolates were phenotypically similar to helicobacter pylori. the isolates were gram-negative, spiral bacteria which grew under microaerophilic conditions at 37 degrees c, but not at 25 or 42 degrees c, and produced urease, catalase, oxidase, alkaline phosphatase, and gamma-glutamyl transpeptidase. the isolates did not ferment glucose, mannitol, inositol, sorbitol, r ...19938379970
pathogenesis of campylobacter fetus infections: critical role of high-molecular-weight s-layer proteins in virulence.wild-type campylobacter fetus strains possess high-molecular-weight s-layer proteins (s+) and are highly resistant to serum-mediated killing and phagocytosis. spontaneous mutant strains lacking these proteins (s-) are serum and phagocytosis sensitive and have reduced virulence in a mouse model. intact s+ cells were treated with pronase, which made them s- although genotypically s+ and had essentially no effect on other cellular proteins or on viability. treatment with pronase, but not buffer alo ...19938421171
[a case of campylobacter fetus subspecies fetus meningitis].a 40-year-old male with no history of underlying disease was admitted to hokusho central hospital on may 25, 1991, complaining of high fever and headache. physical examination on admission revealed a temperature of 38.5 degrees c, a pulse rate of 84 beat/min (relative bradycardia) and no abnormal findings for the chest or abdomen. slight neck stiffness without kernig's sign was observed at neurological examination. laboratory data were: esr 11 mm/lh, wbc 12000/mm3, c-reactive protein positive. l ...19938450275
reproductive performance of beef heifers: effects of vulvo-vaginitis, ureaplasma diversum and prebreeding antibiotic administration.a group of 450 heifers, 13 to 15 mo of age, were individually identified, vaccinated (ibr, pi(3), leptospira, campylobacter fetus), examined (body condition score, reproductive tract evaluation, assessment of vaginal lesions), and cultured for u. diversum . heifers were randomly allocated to either a treated group given chlortetracycline (approximately 350 mg/hd/d for 30 d in a grain crumble) or a nontreated control group. prebreeding, most heifers showed signs of vulvovaginitis, 44% cultured po ...199316727333
campylobacter fetus diarrhea in a hutterite colony: epidemiological observations and typing of the causative organism.following a case of campylobacter fetus sepsis and meningitis in a 4-month-old female member of a hutterite colony, an epidemiological investigation revealed at least 18 cases of diarrhea in other members of the colony. c. fetus was isolated from 7 of 15 fecal samples submitted from affected persons. a case control study suggested that persons who worked in the abattoir were 2.03 times more likely to have had diarrhea, but none of the risk factors studied were significant. the epicurve of the ou ...19947910829
[campylobacter fetus subsp. fetus in a patient with liver cirrhosis].campylobacter fetus subsp. fetus infections are very rare in adults but can occur in immunocompromised elderly people and in patients with chronic disease. we describe the clinical case of a 52-year-old woman with alcoholic liver cirrhosis, where we isolated c.fetus subsp. fetus from blood culture.19947927478
persistence of campylobacter fetus bacteremia associated with absence of opsonizing antibodies.campylobacter fetus causes systemic infections in immunocompromised hosts. we describe a case in which c. fetus bacteremia apparently relapsed after 7 years in a patient with hypogammaglobulinemia and characterize the serum resistance of the patient's c. fetus strain and the inability of the patient's serum, with and without commercial intravenous immunoglobulin, to opsonize this and another c. fetus strain effectively. the probable presence of a sequestered site of infection in bone, the intrin ...19947929763
persistence of campylobacter fetus subspecies venerealis in experimentally infected heifers. 19947941265
antimicrobial susceptibility of campylobacter fetus subsp. fetus isolated from blood and synovial fluid.campylobacter fetus subsp. fetus is a rare human pathogen, but can cause serious extraintestinal infections. effective antimicrobial agent is required for the therapy, but we have very limited knowledge on the susceptibility of the organism. in this study, the susceptibility of 25 isolates of the organism to 14 antimicrobial agents was tested by an agar dilution method. antimicrobial agents with low mic ranges, in micrograms/ml, were: meropenem < or = 0.25, dirithromycin < or = 0.5, gentamicin < ...19947975740
prosthetic hip joint infection due to campylobacter fetus. 19947989567
campylobacter fetus endocarditis: two case reports and review.we report two cases of campylobacter fetus endocarditis. the first case involved a bicuspid native aortic valve in a 60-year-old woman, and the second involved a prosthetic aortic valve in a 76-year-old woman. no source of infection was identified in either case. despite antibiotic therapy, hemodynamic deterioration necessitated valve replacement; both patients recovered completely. c. fetus is an uncommon cause of human infection but may be responsible for severe illnesses such as endocarditis ...19948011832
studies on inactivation of pathogenic microorganisms in culture media and in bovine semen by photosensitive agents.the application of three photosensitive agents for disinfection of bovine semen was investigated. bovine microbial pathogens suspended in tissue culture medium and/or pbs and also added to bovine semen were exposed to the photosensitive agents followed by irradiation. hematoporphyrin, hematoporphyrin derivative and thiopyronine were effective against bovine herpes virus-1, bovine viral diarrhoea virus, mycoplasma bovigenitalium, mycoplasma canadense, and ureaplasma diversum in culture media. in ...19948017031
campylobacter fetus presenting as a septic pleural effusion: a case report.the authors describe a rare case of campylobacter fetus occurring in a patient who showed no overt evidence of immune incompetence. the patient presented with severe cellulitis of the legs, a chronic low grade fever, and a history of recent exposure to sick calves.19948051585
biochemical characterization of campylobacter fetus lipopolysaccharides.lipopolysaccharides (lps) of five strains of the human and animal pathogen campylobacter fetus were electrophoretically and chemically characterized. analysis with sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that all the strains produced smooth-form lps with o side chains of relatively constant chain length. upon extraction, lps partitioned into both the water and phenol phases of phenol-water extracts, which showed that two chemical species of lps were present in each c. fe ...19948063409
septic abortion with intact fetal membranes caused by campylobacter fetus subsp. fetus.we report a case of septic abortion with intact fetal membranes caused by campylobacter fetus subsp. fetus in an 18-year-old woman who was 9 1/2 weeks pregnant.19948077416
evaluation of cultural methods and selective media for the isolation of campylobacter fetus subsp venerealis from cattle. 19948080409
good's syndrome with a block in the early stage of b cell differentiation and complicated by campylobacter fetus sepsis.a 63-year-old man was admitted for campylobacter fetus sepsis and immunodeficiency syndrome with thymoma (good's syndrome). serological examination demonstrated hypoimmunoglobulinemia. analysis of lymphocyte subsets in the peripheral blood and bone marrow showed marked decreases in the proportion of cells bearing b cell markers. however, there were no abnormalities of cellular immunity. this is a rare case of good's syndrome in japan in which the pathogenic mechanism involved a block in the earl ...19947803919
isolation of campylobacter fetus subspecies fetus from a gluteal abscess. 19947811889
[campylobacter fetus subsp. fetus in a patient with liver cirrhosis]. 19947821945
[campylobacter fetus, an infrequent microbe, as a cause of spontaneous bacterial peritonitis in cirrhosis].campylobacter fetus has been implicated in the etiology of sepsis and bacteriemias in immunosupressed subjects. in a few cases, it has also been reported to be responsible for spontaneous bacterial peritonitis in cirrhotic patients. we describe the clinical picture of a woman with terminal liver cirrhosis who had bacteriemia and spontaneous bacterial peritonitis caused by this agent. we argue about the history of cleansing enemas and their probable role in the development of the infection. we st ...19947848698
localization of the sapa gene on a physical map of campylobacter fetus chromosomal dna.we constructed a physical map of campylobacter fetus tk(+) chromosomal dna digested by either smai, sali, or noti using pulsed-field gel electrophoresis and southern hybridization data. the genome size of c. fetus tk(+) is 2016kb, larger than that reported by the others. to locate the sapa gene, which encodes the surface array protein (sap), on the physical map, we performed southern hybridizations with probes based on the conserved region of the sapa gene. the results showed that more than seve ...19947872836
high-frequency s-layer protein variation in campylobacter fetus revealed by sapa mutagenesis.campylobacter fetus utilizes paracrystalline surface (s-) layer proteins that confer complement resistance and that undergo antigenic variation to facilitate persistent mucosal colonization in ungulates. c. fetus possesses multiple homologues of sapa, each of which encode full-length s-layer proteins. disruption of sapa by a gene targeting method (insertion of kanamycin (km) resistance) caused the loss of c. fetus cells bearing full-length s-layer proteins and their replacement by cells bearing ...19947885229
[campylobacter fetus subspecies fetus endoaortitis on a bentall tube prosthesis. apropos of a case].campylobacter fetus is a rare cause of endocarditis and endoaortitis: the authors believe this to be the second reported case of infection of an intracardiac prosthesis. the patient was a man who had already undergone replacement of the aortic valve and ascending aorta, and a gastrectomy, which were predisposing factors. the portal of entry was not found. the diagnosis was confirmed by positive blood cultures and transoesophageal echocardiography. the outcome was rapidly fatal despite antibiotic ...19947771897
serum antibody response to the superficial and released components of helicobacter pylori.superficial and released components were extracted from six selected helicobacter pylori strains. the protein and antigenic profiles of these extracts were representative of the profiles found most frequently among the clinical strains and included major peptidic fractions at 19, 23.5, 57, 68, 76, 118, and 132 kda and major antigens at 68, 57, and 23.5 kda. immuno-cross-reactions were seen with a hyperimmune rabbit serum to campylobacter fetus but not with sera to campylobacter jejuni or salmone ...19947496968
[two cases of campylobacter fetus subsp. fetus sepsis].we encountered two relatively rare cases of sepsis due to campylobacter fetus subsp. fetus (c. fetus). case 1. a 54-year-old female with abdominal polysurgery developed a slight fever and vomiting in august 1984. despite the administration of some digestive drugs by her family doctor, these symptoms continued. in mid-october, she was hospitalized with high fever with chill and rigor on the skin. on the third hospital day, c. fetus was detected in the blood culture. after combination chemotherapy ...19948151152
identification of campylobacter fetus by pcr-dna probe method.a pcr method for rapid identification of campylobacter fetus subsp. fetus was evaluated. a fragment of the gene coding for 16s rrna was amplified from crude cell lysates of 18 c. fetus strains and 30 strains representing other campylobacter species and subspecies. the amplicons were probed by dot blot hybridization with a digoxigenin-labeled c. fetus-specific oligonucleotide probe. the probe reacted only with c. fetus subsp. fetus and c. fetus subsp. venerealis and may be useful for rapid identi ...19957542273
promising new carbapenem antibiotics for treatment of neonatal meningitis due to campylobacter fetus. 19957561257
genome map of campylobacter fetus subsp. fetus atcc 27374.a physical map of the chromosome of campylobacter fetus subsp. fetus was constructed by using pulsed-field gel electrophoresis of restriction fragments generated by sali, smai and noti. digestion of the type strain atcc 27374 with these restriction endonucleases resulted in generating 4-14 fragments. the order of the fragments was deduced from hybridization of these restriction fragments to southern blots of pulsed-field gel electrophoresis gels generated by the other two enzymes. the estimated ...19957590179
in vivo pefloxacin-resistant campylobacter fetus responsible for gastro-intestinal infection and bacteremia associated with arthritis of the hip.the authors report a case of campylobacter fetus subsp. fetus gastro-intestinal infection and bacteremia with poly-arthritis, mainly of the hip, in a french patient simultaneously suffering from cirrhosis of the liver. the outcome was eventually favorable, however only after a trial of ineffective pefloxacin-gentamicin therapy. the authors suggest: (i) gentamicin should not be given alone in c. fetus subsp. fetus infections, and (ii) pefloxacin should not be given if antibiotic sensitivities dat ...19957618366
iatrogenic compressive lumbar myelopathy and radiculopathy in adult cattle following injection of an adjuvanted bacterin into loin muscle: histopathology and ultrastructure.compressive lumbar myelopathy is a recognized iatrogenic complication of injecting water-in-oil vaccines into paravertebral sites of laboratory animals and chickens. herein, we report the histologic and ultrastructural features of a similar complication in a herd of cattle. iatrogenic posterior paresis developed over 34 days in 56 of 610 cows (9.2%) following injection of a commercial bacterin 11-34 days earlier into m. longissimus lumborum. the bacterin was composed of inactivated escherichia c ...19957619907
analysis of strains of campylobacter fetus by pulsed-field gel electrophoresis.campylobacter fetus chromosomal dna from 21 strains was analyzed by pulsed-field gel electrophoresis. the fingerprint patterns generated with smai and sali were distinctive. using the profiles obtained by pulsed-field gel electrophoresis, we established the phylogenetic dendrogram of c. fetus to identify the genetic relationship of the strains.19957650215
a survey of campylobacter bacteremia in three danish counties, 1989 to 1994.over a 5-year period (april 1989 to march 1994) bacteremia caused by campylobacter species was diagnosed in 15 patients in a population of 1.3 million in three counties in jutland province, denmark. the incidence was 0.2/100,000/year and the ratio of blood to fecal isolates, was 0.008. campylobacter coli and campylobacter jejuni accounted for 6 cases each, campylobacter fetus for 2, and in 1 case the species diagnosis was uncertain. seven patients were males and 8 females; median age was 42 (ran ...19957660078
segmental conservation of sapa sequences in type b campylobacter fetus cells.campylobacter fetus cells may exist as either of two defined serogroups (type a or b) based on their lipopolysaccharide (lps) composition. wild-type strains contain surface array proteins (s-layer proteins) that have partial antigenic cross-reactivity but bind exclusively to lps from homologous (type a or b) cells. type a cells possess 8 homologs of sapa, which encodes a 97-kda s-layer protein; the gene products of these homologs have a conserved n terminus of 184 amino acids. to further explore ...19957797493
protein shift and antigenic variation in the s-layer of campylobacter fetus subsp. venerealis during bovine infection accompanied by genomic rearrangement of sapa homologs.campylobacter fetus subsp. venerealis isolated from a case of human vaginosis was inoculated into the uterus of a c. fetus-negative heifer. isolates obtained weekly from the vaginal mucus exhibited variations in high-molecular-mass-protein profiles from that of the original inoculum, which had a dominant 110-kda s-layer protein. immunoblots of the weekly isolates with monoclonal antibody probes against the 110-kda s-layer protein and other c. fetus s-layer proteins demonstrated antigenic shifts. ...19957721688
interleukin-8 response of gastric epithelial cell lines to helicobacter pylori stimulation in vitro.gastric infection with helicobacter pylori activates a mucosal inflammatory response by mononuclear cells and neutrophils that includes expression of cytokines interleukin-1 beta (il-1 beta), il-6, tumor necrosis factor alpha, and il-8. in this study, we analyzed the il-8 response of human gastric cancer cell lines (kato iii, ags, and mkn28) to h. pylori infection in vitro. il-8 mrna expression was detected by reverse transcription-pcr amplification of rna extracted from epithelial cells after i ...19957729872
a lipopolysaccharide-binding domain of the campylobacter fetus s-layer protein resides within the conserved n terminus of a family of silent and divergent homologs.campylobacter fetus cells can produce multiple s-layer proteins ranging from 97 to 149 kda, with a single form predominating in cultured cells. we have cloned, sequenced, and expressed in escherichia coli a sapa homolog, sapa2, which encodes a full-length 1,109-amino-acid (112-kda) s-layer protein. comparison with the two previously cloned sapa homologs has demonstrated two regions of identity, approximately 70 bp before the open reading frame (orf) and proceeding 550 bp into the orf and immedia ...19957896695
bactericidal effects of ozone at nonspermicidal concentrations.a study was conducted to assess the use of ozone (o3) to control pathogens or contaminants of concern to animal breeders and regulatory officials. in separate experiments, samples of fresh bovine semen and pseudomonas aeruginosa, escherichia coli, or campylobacter fetus subsp. venerealis were diluted with antibiotic-free milk (10(6) sperm and 10(6) organisms/ml of diluted semen), exposed in the previous day to a constantly monitored level of 5, 10, 15, or 20 micrograms/ml of o3 for 3-5 min. afte ...19958521350
a detection assay for campylobacter fetus in bovine semen by restriction analysis of pcr amplified dna.a rapid screening assay for campylobacter fetus in bull semen was developed using the polymerase chain reaction (pcr) and restriction endonuclease analysis (rea) to complement isolation by culture. an oligonucleotide primer pair (c1/c2) from the hypervariable region of 16s rrna of c. fetus was used to amplify a 362 base pair fragment by pcr. the pcr/rea assay, which is completed in 10 hours, detected as few as three c. fetus subsp. venerealis cells in experimentally infected raw bull semen and i ...19958540237
[abdominal aortic aneurysm infected with campylobacter fetus spp fetus. report of a case and review of the literature].aortic aneurysm infected with campylobacter fetus spp fetus is rare, the first case having been reported in 1971. we present a case of abdominal aortic aneurysm, with a history of abdominal pain, fever and chills, with identification of this gram negative bacillus in the culture of the aortic wall and visualization of the microorganism in histological examination. surgical correction was performed by interposition of a dracon prosthetic graft. the patient had a good postoperative course, receivi ...19958578095
campylobacter fetus subspecies fetus septicaemia: sds-page as an aid to speciation.we describe a case of bacteraemia due to an atypical strain of campylobacter fetus ssp. fetus. conventional biochemical and phenotypic characterisation was unhelpful but whole cell protein profiles obtained by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) allowed us to identify this strain.19958586844
southern blotting analyses of strains of campylobacter fetus using the conserved region of sapa.chromosomal dna of 27 strains of campylobacter fetus was analyzed by southern blotting with a probe of the conserved region of sapa. the probe hybridized with 23 strains that produced type a lipopolysaccharide. these strains had more than six sapa homologs. in southern blots of sali-digested chromosomal dna separated by pulsed-field gel electrophoresis, one fragment from 19 strains and two fragments from 4 strains hybridized. these data indicate that multiple sapa homologs are localized to a lim ...19958588747
the o-specific polysaccharide chain of campylobacter fetus serotype b lipopolysaccharide is a d-rhamnan terminated with 3-o-methyl-d-rhamnose (d-acofriose).an o-specific polysaccharide was liberated from campylobacter fetus subsp. fetus serotype b lipopolysaccharide by mild acid hydrolysis followed by gel chromatography. this polysaccharide was found to contain d-rhamnose and 3-o-methyl-d-rhamnose (d-rha3me, d-acofriose) in a ratio of approximately 24:1, as well as lipopolysaccharide core constituents. the structure of the polysaccharide was studied by 1h-nmr and 13c-nmr spectroscopy, which included two-dimensional cosy, rotating-frame noe spectros ...19968706751
generation of campylobacter fetus s-layer protein diversity utilizes a single promoter on an invertible dna segment.wild-type strains of campylobacter fetus contain a monomolecular array of surface layer proteins (slps) and vary the antigenicity of the predominant slp expressed. reciprocal recombination events among the eight genomic slp gene cassettes, which encode 97- to 149 kda slps, permit this variation. to explore whether slp expression utilizes a single promoter, we created mutant bacterial strains using insertional mutagenesis by rescue of a marker from plasmids. experimental analysis of the mutants c ...19968730866
[acute hepatitis caused by campylobacter fetus]. 19968763076
an environmentally regulated pilus-like appendage involved in campylobacter pathogenesis.examination of strains of campylobacter jejuni, campylobacter coli, and campylobacter fetus by electron microscopy revealed that they produced peritrichous pilus-like appendages when the bacteria were grown in the presence of bile salts. various bile-salt supplements were used and it was found that deoxycholate and chenodeoxycholic acid caused a significant enhancement of pilus production and resulted in a highly aggregative phenotype. morphologically, the pili were between 4 and 7 nm in width a ...19968793884
infective endocarditis caused by campylobacter fetus after allogeneic tooth transplantation: a case report.a patient developed infective endocarditis caused by campylobacter fetus. he gave a history of recent dental extraction and allogeneic tooth transplantation. he was treated with various antibodies to which the organism was said to be sensitive, but it was not until the transplanted tooth was removed that he started to improve. the mode of infection was thought to be blood borne through the open tooth socket from the raw chicken that he ate regularly.19968818256
electrophoretic and immunoblot analysis of campylobacter fetus lipopolysaccharides.proteinase k-digested cell lysates from 25 campylobacter fetus subspecies fetus and c. fetus subsp. venerealis strains were examined by sds-page and immunoblotting. three sds-page lipopolysaccharide (lps) profiles were observed. two profiles were consistent with those previously reported for serogroup a and serogroup b and ab isolates and were distinguished by the relative mobility of bands in the o-chain region and by a strong reaction on immunoblots with homologous antisera. the third profile ...19968828127
epidemiologic application of pulsed-field gel electrophoresis to an outbreak of campylobacter fetus meningitis in a neonatal intensive care outbreak of nosocomial campylobacter fetus meningitis occurred in a neonatal intensive care unit (nicu). eight c. fetus strains were isolated from 4 infants with meningitis, the mother of the index patient and 2 infants who were asymptomatic intestinal carriers. the pulsed-field gel electrophoresis (pfge) pattern with the restriction endonucleases smal and sall were found to be identical for the nosocomial c. fetus isolates, but the patterns were different from those of sporadic strains. thes ...19968863359
biological characterization of campylobacter fetus lipopolysaccharides.lipopolysaccharides (lps) of three strains of campylobacter fetus (subspp. fetus and venerealis, and serotypes a and b), a bacterium of veterinary importance but also a cause of various infections in humans, were assessed for their ability to induce mitogenicity, gelation of limulus amebocyte lysate, lethal toxicity in mice, and pyrogenicity in rabbits. all c. fetus lps exhibited activities lower than those of salmonella typhimurium lps. lps of c. fetus subsp. fetus serotype a had the lowest act ...19968871115
expression, secretion and antigenic variation of bacterial s-layer proteins.the function of the s-layer, a regularly arranged structure on the outside of numerous bacteria, appears to be different for bacteria living in different environments. almost no similarity exists between the primary sequences of s-proteins, although their amino acid composition is comparable. s-protein production is directed by single or multiple promoters in front of the s-protein gene, yielding stable mrnas. most bacteria secrete s-proteins via the general secretory pathway (gsp). translocatio ...19968898381
[campylobacter fetus meningitis in adults].campylobacter fetus is an uncommon cause of meningitis in the adult. we report a case observed in an 84-year-old man with alcoholic cirrhosis. the patient presented fever, jaundice and a state of mental confusion. blood and cerebrospinal fluid cultures identified campylobacter fetus sensitive to several antibiotics. ciprofloxacine-ceftriaxone combination replaced the antibiotics prescribed empirically prior to identification and led to regression of the fever and normal mental status within 4 da ...19968942943
tactical control of ovine campylobacter abortion outbreaks with a bacterin.abortions due to campylobacter fetus fetus (c. fetus fetus) were diagnosed in three canterbury ewe flocks 6 weeks prior to lambing. in two of the flocks, two inoculations of a c. fetus fetus bacterin, 10 days apart, reduced the incidence of abortions in the treated ewes to about one third and one half respectively of the level in the control ewes in the same flock. the treatment had no effect in a third flock where an outbreak had been in progress for 2 weeks before investigations started. the r ...199616031895
attempted definition by immunoblotting of the causes of reactivity in suspected false-positive sera in the brucella ovis complement fixation test.seventy-nine suspected false-positive sera, obtained over 1 year from routine submissions for brucella ovis serological testing, were used in this study. these sera, which exhibited titres in the complement fixation test, but which because of their epidemiological history and their reactions in the enzyme-linked immunosorbent assay and gel diffusion test were suspected to be false positives, were further analysed by immunoblotting. in blots, using b. ovis antigens, rough lipopolysaccharide was i ...199616031926
purification and characterization of campylobacter rectus surface layer proteins.campylobacter rectus is a putative periodontopathogen which expresses a proteinaceous surface layer (s-layer) external to the outer membrane. s-layers are considered to play a protective role for the microorganism in hostile environments. the s-layer proteins from six different c. rectus strains (five human isolates and a nonhuman primate [nhp] isolate) were isolated, purified, and characterized. the s-layer proteins of these strains varied in molecular mass (ca. 150 to 166 kda) as determined by ...19979009300
nested dna inversion as a paradigm of programmed gene rearrangement.programmed gene rearrangements are employed by a variety of microorganisms, including viruses, prokaryotes, and simple eukaryotes, to control gene expression. in most instances in which organisms mediate host evasion by large families of homologous gene cassettes, the mechanism of variation is not thought to involve dna inversion. here we report that campylobacter fetus, a pathogenic gram-negative bacterium, reassorts a single promoter, controlling surface-layer protein expression, and one or mo ...19979023369
cloning and nucleotide sequence of the gyra gene from campylobacter fetus subsp. fetus atcc 27374 and characterization of ciprofloxacin-resistant laboratory and clinical isolates.the gyra gene of campylobacter fetus subsp. fetus, which encodes the a subunit of dna gyrase, was cloned, and its nucleotide sequence was determined. an open reading frame of 2,586 nucleotides which encodes a polypeptide of 862 amino acids with an mr of 96,782 was identified. c. fetus subsp. fetus gyra is most closely related to campylobacter jejuni gyra, with 73% homology at the nucleotide level and 78% identity between polypeptides. the next most closely related gyra was that from helicobacter ...19979056011
Displaying items 1001 - 1100 of 1841