TitleAbstractYear(sorted ascending)
structural and biochemical analyses of a surface array protein of campylobacter fetus.electron microscopic examination of ultrathin sections and freeze-etched and shadow cast preparations of a bovine prepuce isolate of campylobacter fetus vc119 showed an s layer with subunits in an apparent linear arrangement. surface radioiodination, enzyme digestion, low-ph extraction, and western immunoblotting showed that the layer was composed mainly of one protein which is the predominant protein antigen of c. fetus. this protein was purified to homogeneity by gel filtration, ion-exchange c ...19883410826
colonization of gastrointestinal tracts of chicks by campylobacter jejuni.bacterial enumeration and histologic examination of organs and tissues of 8-day-old chicks 7 days after peroral inoculation with campylobacter jejuni revealed that the organism colonized primarily the lower gastrointestinal tract. the principal sites of localization were the ceca, large intestine, and cloaca, where densely packed cells of c. jejuni were observed in mucus within crypts. examination of c. jejuni-colonized crypts by transmission electron microscopy revealed that the campylobacters ...19883060015
campylobacters in man and the environment in hull and east yorkshire.campylobacter organisms isolated from water samples taken weekly from ponds and land-drains in the city of hull were compared with isolates from humans. of 314 campylobacter organisms isolated from patients, 237 (75.5%) of the strains were identified as typical campylobacter jejuni, whilst of 125 identified strains isolated from the water samples, 85 (68%) resembled c. jejuni in most respects but were hippurate hydrolysis negative by the hwang and ederer method. the ponds and land drains in the ...19883181312
isolation of campylobacter jejuni from the bile of a cholecystic patient.described is the isolation of campylobacter jejuni from the bile of a cholecystic patient admitted for abdominal pain to an intensive care unit. a cholecystectomy was performed, and the patient responded to erythromycin therapy. details of this uncommon isolation are given.19883183005
infection with campylobacter coli and campylobacter fetus in a patient on continuous ambulatory peritoneal dialysis. 19883183412
detection of two different kanamycin resistance genes in naturally occurring isolates of campylobacter jejuni and campylobacter coli.a total of 225 isolates of campylobacter jejuni and 54 isolates of campylobacter coli were screened for resistance to kanamycin. among these, five resistant isolates of c. jejuni and six resistant isolates of c. coli, all with different plasmid patterns, were identified. each contained at least one plasmid greater than or equal to 41 kilobases in size. the mic of kanamycin for all 11 strains was determined to be greater than or equal to 256 micrograms/ml by an agar dilution method. in addition, ...19883190204
is enrichment culture necessary for isolating campylobacter jejuni from faeces? 19883192739
nosocomial meningitis due to campylobacter fetus subsp. fetus in a neonatal intensive care unit. 19883197739
trichomoniasis and campylobacteriosis in bulls in the republic of transkei.trichomonas foetus was demonstrated in 23/87 (26.4%) and campylobacter fetus in 25/87 (28.7%) of bulls tested in transkei. a total of 16/87 (18.39%) of bulls tested were positive for both trichomonas foetus and campylobacter fetus. bulls from 14 sites in transkei were tested and trichomonas foetus was isolated at 9 of these sites. campylobacter fetus was isolated at 10 of the 14 sites. the results indicate that both trichomonas foetus and campylobacter fetus are widespread throughout the cattle ...19883199409
role of the 92.5-kilodalton outer membrane protein of campylobacter jejuni in serological reactions.outer membrane proteins from 12 strains of campylobacter jejuni were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting with rabbit antisera prepared against formalin-treated cell suspensions of three of the strains. the 92.5-kilodalton protein was confirmed to be a minor outer membrane protein and exhibited serological heterogeneity. serological specificities of the proteins, however, were found not to correlate with serotypic specificities of the strains, ...19883230126
paediatric campylobacter diarrhoea from household exposure to live chickens in lima, peru.although campylobacter jejuni is a frequent enteropathogen in cases of paediatric diarrhoea in developing countries, its route of transmission is not well understood. an age-matched, case-control study of children with c. jejuni diarrhoea was therefore carried out in lima, peru, from january 1983 to april 1986 to identify the risk factors and vehicles of transmissions. as cases, 104 children less than 3 years of age were selected and compared with controls of the same age with non-gastrointestin ...19883262442
high-voltage electroporation of bacteria: genetic transformation of campylobacter jejuni with plasmid dna.electroporation permits the uptake of dna by mammalian cells and plant protoplasts because it induces transient permeability of the cell membrane. we investigated the utility of high-voltage electroporation as a method for genetic transformation of intact bacterial cells by using the enteric pathogen campylobacter jejuni as a model system. this report demonstrates that the application of high-voltage discharges to bacterial cells permits genetic transformation. our method involves exposure of a ...19883277182
campylobacter fetus endocarditis on a prosthetic heart valve.campylobacter fetus infection in man is rare. few cases of campylobacter endocarditis have been described, and none have been associated with a prosthetic heart valve. we have reported a case of campylobacter fetus ssp fetus on a prosthetic mitral valve in a 48-year-old woman who was treated successfully with penicillin and streptomycin (and later with gentamicin). replacement of the prosthetic valve was done because of stenosis and vegetations. pathologic examination showed vegetations on a cal ...19883287646
campylobacters and impaired fetal development in mice.intravenous injection of eight human strains of campylobacter fetus ss fetus and campylobacter jejuni into mice at various stages of pregnancy demonstrated significant strain differences in ability to affect implantation of the fertilised ovum and to cause resorption of the mouse fetus. implantation was significantly impaired when c. fetus ss fetus was injected intravenously on day 2 of pregnancy, but no effect was observed in mice receiving c. jejuni. on day 6 of pregnancy, before the developme ...19882961890
the effect of campylobacter lipopolysaccharide on fetal development in the mouse.purified lipopolysaccharide (lps) obtained from isolates of campylobacter fetus ss. fetus and campylobacter jejuni impaired fetal development when administered to mice on day 13 of pregnancy. strikingly more fetal resorption was produced by c. jejuni lps than by similar amounts of c. fetus ss. fetus lps. three of the four campylobacter strains examined produced lps that had no effect on maternal health, but lps from one c. jejuni strain killed all of the mice to which it was administered.19882968457
gene disruption and replacement as a feasible approach for mutagenesis of campylobacter jejuni.campylobacter jejuni and campylobacter coli are important causes of human enteric infections. several determinants of pathogenicity have been proposed based on the clinical features of diarrheal disease and on the phenotypic properties of campylobacter strains. to facilitate an understanding of the genetic determinants of campylobacter virulence, we have developed a method for constructing c. jejuni mutants by shuttle mutagenesis. in the example described here, a kanamycin resistance gene was in ...19882832375
a new antibiotic combination for frozen bovine semen 1. control of mycoplasmas, ureaplasmas, campylobacter fetus subsp. venerealis and haemophilus somnus.systematic evaluations of new combinations of antibiotics for the control of bovine mycoplasmas, ureaplasmas, campylobacter fetus subsp. venerealis and haemophilus somnus in a bovine frozen semen process were made. these organisms were standardized to 10(5) to 10(6) colony forming unit (cfu) and inoculated into each ml of raw semen. antibiotics in a final volume of 0.02 ml were added to each ml of the raw semen and were contained at the same concentration in the nonglycerol portion of the extend ...198816726381
campylobacter jejuni isolations from mexican and swedish patients, with repeated symptomatic and/or asymptomatic diarrhoea episodes.the presence of different campylobacter jejuni serotypes in swedish patients with diarrhoea and in mexican patients with or without diarrhoea was investigated with special reference to repeated isolations during the course of infection and to symptomatic and asymptomatic episodes. the study included 136 c. jejuni isolates from 62 mexican children and 173 isolates from 68 swedish patients. the bacteria were serotyped for heat-stable (hs) and heat-labile (hl) antigen. swedish patients, all with sy ...19892917617
campylobacter jejuni and campylobacter coli diarrhoea in rural and urban populations in yugoslavia.during a 4-month period during the summer of 1985, campylobacters were isolated from 338 (16.3%) of 2080 patients with acute diarrhoea attending the university hospital of infectious diseases in zagreb. of these isolates 220 (64.1%) were campylobacter jejuni and 118 (34.9%) were c. coli. the patients were drawn from three residential zones in and around zagreb: inner city, peripheral city and rural. incidences of campylobacter diarrhoea ranged from 71 per 100,000 per year in inner city residents ...19892917618
campylobacteriosis in an aborted equine fetus.abortion caused by campylobacter fetus subsp fetus was diagnosed in a 7-month-old equine fetus. the fetus was small for its gestational age. macroscopically, the proximal portion of the small intestine was hemorrhagic and its wall was thick. histologically, the brunner glands were distended with neutrophils, and the submucosa was thick, owing to fluid accumulation and/or cellular infiltrates. curved bacteria were observed in the brunner glands and intestinal glands. campylobacter fetus subsp fet ...19892917894
human antibody response to campylobacter jejuni flagellin protein and a synthetic n-terminal flagellin peptide.we measured isotype-specific human antibodies directed against campylobacter jejuni native flagellin and a synthetic peptide derived from the n-terminal amino acid sequence of the protein by using a microdilution enzyme-linked immunosorbent assay (elisa). serum samples from patients with gastrointestinal infection caused by c. jejuni (n = 20) and control samples (number from normal subjects = 20; number from patients with diarrhea other than campylobacter = 20) were tested in this assay. serum s ...19892584372
bacterial infertility in camels (camelus dromedarius): isolation of campylobacter fetus.this study constitutes the first reported isolation of c. fetus subsp. venerealis from cervical swabs in breeding camels. 9 of 30 camels which were barren for a long period were found to be positive for c. fetus. the methods of isolation are described. additionally, cervical flora of 30 camels were examined. 9 different bacterial species were isolated of which staphylococcus aureus was most frequently found. all samples were negative for trichomonas sp. and streptococcus zooepidemicus.19892598805
response of the chick embryo to live and heat-killed campylobacter jejuni injected into the yolk sac.graded doses of live and heat-killed cells of campylobacter jejuni were injected into the yolk-sac of 5-day-old chick embryos, and the 50% lethal dose (ld50) was determined 7 days later. a strain dependent virulence was seen. in the diluted series of cultures the ld50 values for live campylobacter ranged from 10(6) c.f.u. beyond the last dilution showing growth, that is to less than one organism per embryo. when the 22 strains were tested as heat-killed cells, the chick embryo ld50 values retain ...19892606163
[infectious diarrhea diseases].gastroenteritis may be due to many agents, infectious or non infections. most cases of infections diarrhea are selflimited--e.g. food born intoxications with 1 to 2 days duration or infections with enteropathogenic e. coli, salmonella spp., vibrio parahaemolyticus or rota virus. even the majority of shigellosis, salmonella-gastroenteritis and infections with campylobacter fetus cease without treatment within 4 days. fever and diarrhea persisting for more than 4 days justify the use of antibiotic ...19892662618
[neurologic involvement in campylobacter infections. 5 cases].campylobacter are a newly recognized class of human infectious agents. campylobacter fetus subspecies fetus is a cause of sepsis in immunocompromized hosts. secondary neurological determinations, meningitis and meningoencephalitis appear to be rare. we report 2 cases, and 8 previously reported cases are reviewed. campylobacter jejuni appears to be a common bacterial pathogen causing a syndrome of enteritis. since 1982 it has been associated with guillain-barré syndrome (gbs). in one serological ...19892664975
campylobacter fetus subspecies fetus infection of an abdominal aneurysm.we report a case of aneurysm of the abdominal aorta with spontaneous rupture in a 64-year-old man with emphysema and chronic alcoholism. campylobacter fetus subspecies fetus was repeatedly recovered from the blood and from specimens of the excised aneurysm. the patient died in spite of early diagnosis, intravenous erythromycin and surgical intervention. the bacteriology and epidemiology of campylobacteriosis in man is briefly discussed and eight other published cases of aneurysm, infected with t ...19892678860
applications of a new method for devising bacteriological serotyping schemes.greatly simplified serotyping schemes were devised for two organisms, escherichia coli and campylobacter jejuni. the mathematical approach developed to improve the e. coli serotyping routine at the national health institute in new zealand was used on three previously published data sets. it had been considered possible that the mathematical method used in the first study was specific to that data set and therefore not applicable elsewhere. this paper verifies that the mathematical method works o ...19892685036
campylobacter fetus subsp. jejuni in poultry reared under different management systems in nigeria.cloacal swabs from 487 live birds in 36 flocks and 70 poultry carcasses were cultured for campylobacter fetus subsp. jejuni. it was isolated from 12.3% of the birds in 19 flocks. chickens, turkeys, and guinea fowl differed from one another in isolation rates of the organism. management system affected its occurrence, and only 7.1% of eviscerated carcasses yielded it. it was concluded that bird species, management system, and immersing slaughtered poultry in boiling water before dressing affect r ...19892695052
[a case report of campylobacter fetus subspecies fetus bacteremia].an unusual case of campylobacter fetus subspecies fetus bacteremia was presented. a twenty four year old male was admitted to our hospital due to abdominal pain, general malaise, diarrhea, high fever, and hemoptysis. he was alcoholic and fond of eating raw liver. he had a history of partial gastrectomy and disturbance of pancreatic function. he showed pulmonary empyema, pleuritis, thrombophlebitis of lower legs, jaundice, hepatomegaly, diarrhea, pneumothorax, and low t3 low t4 syndrome. c. fetus ...19892695582
serotyping studies of campylobacter from naturally colonized chickens.campylobacter jejuni/coli strains from 164 chickens were serotyped by the methods previously described by penner et al. and lior et al. the chickens were sampled during breeding from hatching to the age of 42 weeks. the birds were housed, in two separate groups, under different environmental conditions, (for comparison of the effect of hygienic precautions on the transmission of the bacteria during breeding). in the group where the hygienic conditions could be controlled to a greater extent, the ...19892703018
comparison of different vaccines and induced immune response against campylobacter jejuni colonization in the infant mouse.the degree of protection conferred by vaccinated dams on infant mice against colonization by campylobacter jejuni depended on the bacterial strain, preparation, and route of administration of the vaccine. in some instances of homologous protection, serum bactericidal titres correlated well with protection. however, boiled c. jejuni vaccine, which was non-protective, also elicited a strong bactericidal antibody response. conversely, bactericidal activity could not be demonstrated against strains ...19892703020
population genetics of human and animal enteric campylobacter strains.a total of 125 strains of campylobacter jejuni and campylobacter coli from human and animal hosts were analyzed by multilocus enzyme electrophoresis. ten genetic loci were characterized by the electrophoretic mobilities of their products, and all were found to be polymorphic. the nine loci considered for genetic analysis had an average allele number of 6.9 and a mean genetic diversity of 0.634. the strains were grouped into 64 electrophoretic types (ets) forming two principal clusters; the first ...19892707852
experimental campylobacter jejuni infection in macaca nemestrina.experimental infection of four specific-pathogen-free macaca nemestrina monkeys (aged 3.5 and 4.5 months) with campylobacter jejuni 81-176 caused acute diarrheal illness, characterized by fluid diarrhea, bloody stools, and fecal leukocytes, which lasted for approximately 7 to 11 days. histologic examination of intestinal biopsies showed acute colitis characterized by infiltration of the mucosa with neutrophils and lymphocytes, and cryptitis. there were no histologic changes in the small intestin ...19892707853
campylobacter fetus as a pulmonary pathogen in man. case report of post-pneumonectomy empyema. 19892710441
cellular events and intracellular survival of campylobacter jejuni during infection of hep-2 cells.invasion and intracellular survival of campylobacter jejuni in hep-2 cells were analyzed by transmission electron microscopy and by viable counts after killing of extracellular bacteria by gentamicin. during the first 30 min after challenge, no bacteria were seen in association with the host cell. after 1 h, campylobacters apparently attached to the cell membrane, with areas of close appositions. in these areas, an intracellular network of actin-like filaments was seen beneath the plasma membran ...19892731988
colonization of mice by campylobacter jejuni.both streptomycin-treated and untreated swiss white mice were irregularly colonized when challenged orogastrically with between 1 and 10(11) viable organisms of either of two strains of campylobacter jejuni. the organisms were occasionally recovered from portions of the intestinal tracts of these animals in numbers ranging from 10(1) to 10(3)/g when the challenge doses were 10(10) or more. when germfree mice were challenged with 10(8) organisms of either strain, the entire intestinal tracts of a ...19892744846
immune response to campylobacter jejuni and campylobacter coli in a cohort of children from birth to 2 years of age.a cohort of 111 children from bangui, central african republic, was surveyed for enteric campylobacter infections from birth to the age of 2 years; stools were examined biweekly in these children until 6 months of age and at least four times per year thereafter until 2 years of age and after each diarrheal episode. blood samples were obtained at birth and at 3, 6, 9, 12, 18, and 24 months of age. antibodies against glycine-extracted membrane antigens, purified flagella, and cholera toxin (ct) we ...19892744860
hexagonal surface layer of campylobacter fetus isolated from humans.electron microscopic studies of the surface structure of campylobacter fetus by the freeze-etching method showed two different types of s layer. one was in a hexagonal array, and the other was in a tetragonal array. a high-passage-number strain lost its s layer during cultivation on culture media but regained it after a single animal passage.19892744862
identification of campylobacter jejuni and c. coli by gel electrophoresis of the outer membrane proteins.analysis of the electrophoretic profiles of the outer membrane proteins could be used to differentiate campylobacter jejuni (16 strains) from campylobacter coli (10 strains). this observation was confirmed by the study of dna homology obtained by a quantitative filter hybridization method. the hippurate hydrolysis test gave a poor correlation with the results of differentiation obtained by dna homology studies and outer membrane protein profile.19892745680
cellular fatty acid composition of campylobacter pylori from primates and ferrets compared with those of other campylobacters.the cellular fatty acid profiles of newly described campylobacters were determined on a polar, capillary column. six isolates of the gastric spiral organism, campylobacter pylori subsp. mustelae, from ferrets from australia, england, and the united states were all found to have a similar fatty acid profile which was different from that of c. pylori from humans; c. pylori subsp. mustelae did not have 3-hydroxyoctadecanoic acid (3-oh c18:0) and had much less tetradecanoic acid (c14:0) and much mor ...19892745703
cases of infective endocarditis caused by campylobacter 18-year-old man with infective endocarditis caused by campylobacter fetus is reported. this bacterial species has long been of interest in veterinary medicine, and recently it has been reported to be one cause of infantile diarrhea. infective endocarditis arising from campylobacter fetus is rare. this is the fourth case reported in japan. the organism was resistant to several antibiotics, and large vegetations were found to involve the aortic valve. emergency excision of the infected aortic v ...19892748151
enterotoxin production and serogroups of campylobacter jejuni and campylobacter coli from patients with diarrhea and from healthy laying hens.enterotoxin production, a possible virulence factor, was determined in campylobacter jejuni and campylobacter coli by two different techniques, the cho cell test and the gm1 enzyme-linked immunosorbent assay. the frequency of enterotoxigenic campylobacter strains was 32% in strains from both humans with acute enteritis and healthy laying hens, as measured by the cho cell test. the cho cell test was significantly more sensitive than the gm1 enzyme-linked immunosorbent assay in the detection of en ...19892754001
virulence of campylobacter jejuni for chicken embryos.the pathogenicity of campylobacter jejuni was examined in chicken embryos. in this system, mortality data and histopathological findings induced by organisms and by bacterium-free filtered broth were identical. the absence in chicken embryo tissues both of organisms and of an inflammatory infiltrate suggests a toxin etiology.19892754005
effects of sample holding time, temperature, and atmosphere on the isolation of campylobacter jejuni from dogs.stool specimens were collected from 39 dogs, inoculated onto campylobacter blood agar plates, and divided into four subsamples. subsamples were held at 4 and 25 degrees c in room air and in a microaerobic environment and were reinoculated at 1, 2, 3, 4, 6, and 8 h. c. jejuni survived at least 3 h when it was held at 4 degrees c, but less than 2 h when it was held at 25 degrees c. the holding atmosphere was not associated with a difference in isolation rates.19892754014
waterborne outbreak of campylobacter enteritis after outdoors infantry drill in utti, finland.diarrhoea, abdominal pain, malaise and fever affected 75 of the 88 conscript soldiers in utti, finland after an outdoors infantry drill. campylobacter jejuni, heat-stable serotype 3/43/59, was isolated from 37 out of 63 men investigated. a clear serological response was evident in the risk group and negligible in the control group. the entire population at risk was interviewed. the outbreak was associated with the consumption of untreated surface water. c. jejuni, heat-stable serotype 3/43/59, w ...19892776848
interaction of campylobacter species with antibody, complement and phagocytes.the opsonisation of four different campylobacter species for human neutrophils was studied using a chemiluminescence system and electron microscopy. opsonisation of campylobacter fetus, campylobacter coli, and campylobacter jejuni was mediated by antibody and enhanced by complement. antibody was not, however, required for the phagocytosis of campylobacter pylori because it activates the classical pathway of complement directly. this unusual property may be important in the pathogenesis of c pylo ...19892788111
in-vitro evaluation of antibiotic combinations against campylobacter fetus.we evaluated the in-vitro activity of 16 antibiotics against 22 clinical isolates of campylobacter fetus. the interaction of these antibiotics was also tested by chequer-board titration and time-kill assay. eight antibiotic combinations were evaluated. antagonism was not found with any of the combinations tested. the highest synergistic effect was obtained with a combination of ampicillin and cefazolin or ampicillin and gentamicin.19892793642
in vitro evaluation of antibiotics for treatment of meningitis caused by campylobacter fetus subspecies fetus. 19892797963
campylobacter fetus cholangitis and bacteremia. 19892804850
early treatment of campylobacter jejuni enteritis.the bacteriologic and clinical effects of early antibiotic treatment of campylobacter jejuni enteritis were studied. erythromycin rapidly eliminated c. jejuni from stools, whereas trimethoprim-sulfamethoxazole did not. despite its bacteriologic effectiveness, erythromycin did not reduce the duration or severity of diarrhea, abdominal pain, or other symptoms.19892818711
experimental production of proliferative ileitis in syrian hamsters (mesocricetus auratus) by using an ileal homogenate free of campylobacter jejuni.the role of campylobacter jejuni in the pathogenesis of proliferative ileitis of syrian hamsters (mesocricetus auratus) has been uncertain. c. jejuni has been implicated as the etiologic agent on the basis of the campylobacter-type morphology of the intracellular organism and the repeated microbiologic isolation of c. jejuni from hamsters with proliferative ileitis. the inability to reproduce the disease with pure culture inocula, coupled with immunohistochemical studies, however, has suggested ...19892462537
flagellin expression in campylobacter jejuni is regulated at the transcriptional level.campylobacter jejuni 81116 is able to switch flagellum formation on and off. to study the expression of flagellin, the main component of flagella, an expression library of c. jejuni dna was constructed in lambda gt11. screening of this library with a flagellin-specific antiserum resulted in a clone producing a beta-galactosidase-flagellin fusion protein; it contained a dna insert of 850 base pairs and coded for about 15 kilodaltons of the flagellin, corresponding to 410 base pairs of the flagell ...19892466792
prospective study of campylobacter jejuni infection in chilean infants evaluated by culture and serology.a prospective study of campylobacter jejuni infection was performed during a 6-month period in a cohort of 198 chilean infants. surveillance was based on biweekly home visits by a team of trained nurses. c. jejuni colonization was studied by culturing stool samples from diarrheal episodes and paired asymptomatic controls and by performing an enzyme-linked immunosorbent assay developed to measure c. jejuni outer membrane protein polyvalent antibodies in a representative group of infants. c. jejun ...19892473090
[sepsis caused by campylobacter fetus spp. fetus]. 19892491527
pancreatitis associated with campylobacter jejuni infection: diagnosis by ultrasonography. 19892499366
[the most frequent infectious causes of abortion in sheep in north bavaria with special reference to chlamydia and salmonella infections].between 1980 and 1987 1153 ovine fetuses and placentas were examined after abortion. in 68.5% of the cases a cause of abortion could be diagnosed as follows: 43.5% chlamydia psittaci var. ovis, 10.7% salmonella abortus ovis, 3.7% coxiella burnetii, 3.3% listeria monocytogenes and 0.4% campylobacter fetus intestinalis. hemolyzing escherichia coli and streptococci, yersinia pseudotuberculosis, salmonella typhimurium, salmonella enteritidis, corynebacterium pyogenes and other facultative pathogens ...19892503908
rapid method for the detection of dnase of campylobacters.a rapid agar diffusion method for the detection of dnase production of campylobacter jejuni, c. coli, and c. pylori was developed. a strong pink zone indicating dna hydrolysis was seen around the wells after 20 to 24 h of aerobic incubation at 37 degrees c. pretreatment of cells with polymyxin b, which releases the cell-associated dnase, both shortened the time needed to read positive results to 8 h and increased the zone size.19892506224
abdominal aortic aneurysm infected with campylobacter fetus subspecies fetus.we report a survivor of campylobacter fetus septicemia from an infected abdominal aortic aneurysm who was successfully treated with an anatomic graft reconstruction and antibiotics. according to a survey of the english-language medical literature this was the fourth such patient successfully treated. c. fetus sepsis associated with an abdominal aortic aneurysm was first reported in 1971. the first patient to survive reconstruction of an aortic tube graft aneurysm infected with c. fetus was repor ...19892527318
antigenic and restriction enzyme analysis of isolates of campylobacter fetus subsp venerealis recovered from persistently infected cattle.thirty-two isolates of campylobacter fetus subsp venerealis were obtained from 1 bull and 4 heifers with experimentally induced infection. when whole-cell antigens of isolates were cross titrated with antisera to the infecting strain, isolates from 3 heifers had limited antigenic variation, whereas whole-cell antigens of isolates from 2 cattle (the bull and a heifer) differed serologically from those of the infecting strain. changes were detected specifically in 6 heat-labile antigens. of the 6 ...19892548417
adhesion to and invasion of hep-2 cells by campylobacter spp.twenty-one isolates were tested for their ability to adhere to and invade hep-2 cells in vitro. of the 21 organisms tested, 2 did not invade the hep-2 cells, and 1 of these did not adhere to the epithelial cells. campylobacter jejuni clinical isolates were more invasive than the nonclinical strains that were tested. when hep-2 cells were treated with cytochalasin b, the invasiveness of c. jejuni was reduced, indicating active participation of the host cell in the uptake of these organisms. the n ...19892550368
antigenic differences among campylobacter fetus s-layer proteins.sodium dodecyl sulfate-polyacrylamide gel electrophoresis of s-layer proteins extracted from campylobacter fetus strains by using acid glycine buffer showed that the predominant s-layer proteins of different strains had subunit molecular weights in the range of 90,000 to 140,000. electron microscopy revealed oblique s-layer lattices with a spacing of approximately 5.6 nm (gamma = 75 degrees) on wild-type strains vc1, vc119, vc202, and vc203. three variants of c. fetus vc119 producing a predomina ...19901697574
campylobacter fetus subspecies fetus meningitis with chronic alcoholism and diabetes mellitus.a case of campylobacter fetus subspecies fetus meningitis is reported. the patient had underlying diseases, namely chronic alcoholism and diabetes mellitus. the infection did not respond to piperacillin and cefotaxime, but did respond to ampicillin and moxalactam. the patient was discharged on the 33rd hospital day showing no neurological deficit complications, and has remained free of recurrent disease for one month after the discontinuation of therapy.19902089181
[bacteremia caused by campylobacter fetus ssp fetus in a patient with liver cirrhosis]. 19902090246
[bacteremia caused by campylobacter fetus ssp fetus in an hiv-positive patient with liver cirrhosis]. 19902090247
adherence of helicobacter pylori cells and their surface components to hela cell membranes.four helicobacter pylori strains were used to develop in vitro methods to assess adherence to hela cells. using direct detection by microscopy, adhesion scores increased with the initial bacteria-to-cell ratio. the urease method assessed h. pylori bound to hela cells by their urease activity. the percentage of the original inoculum adhering to hela cells remained constant for initial ratios from 10(2) to 10(5) bacteria per cell. an elisa using anti-h. pylori serum assessed whole bacteria or comp ...19902097496
[pericarditis caused by campylobacter fetus fetus]. 19902099869
microbiological quality and safety of zoo food.two types of commercial products for feeding zoo animals (a frozen meat product, referred to as zoo food, and a dry product, referred to as dry food) were microbiologically examined for spoilage organisms (aerobic, psychrotrophic, coliform, escherichia coli, mold, and yeasts) and pathogens (salmonella spp., listeria monocytogenes, and campylobacter jejuni). levels of microorganisms in frozen ground zoo food were compared with those in frozen ground beef and frozen ground turkey meat. the level o ...19902111115
hydrophobic characterisation of helicobacter (campylobacter) pylori.cell-surface hydrophobicity of helicobacter (formerly campylobacter) pylori was tested by aqueous two-phase partitioning and hydrophobic interaction chromatography. the hydrophobicity of h. pylori greatly exceeded that of campylobacter fetus subsp. fetus, c. jejuni and bacillus subtilis. a partition coefficient (pc) of hydrophobicity in the two-phase system was determined for h. pylori. pc was dependent on ph and the pc value was increased by greater than 20-fold at ph 2.5. lithium cations incre ...19902113100
identification of campylobacter jejuni surface proteins that bind to eucaryotic cells in understand the role of campylobacter jejuni surface proteins in the interaction of c. jejuni with cultured mammalian cell lines in vitro, we developed a ligand-binding assay. this procedure allowed us to antigenically identify c. jejuni outer membrane proteins (omps) that attach to intact host cell membranes. omps isolated from an invasive strain and a less invasive strain were antigenically indistinguishable. however, we found that proteins with molecular masses of 28 and 32 kilodaltons (kda ...19902160431
use of pulsed-field agarose gel electrophoresis to size genomes of campylobacter species and to construct a sali map of campylobacter jejuni determine the physical length of the chromosome of campylobacter jejuni, the genome was subjected to digestion by a series of restriction endonucleases to produce a small number of large restriction fragments. these fragments were then separated by pulsed-field gel electrophoresis with the contour-clamped homogeneous electric field system. the dna of c. jejuni, with its low g+c content, was found to have no restriction sites for enzymes noti and sfii, which cut a high-g+c regions. most of the ...19902168376
cardiovascular and bacteremic manifestations of campylobacter fetus infection: case report and review.a case of bacteremia due to campylobacter fetus subspecies fetus with concomitant pleuropericarditis in a previously healthy patient is presented. the organism is ubiquitous, but most commonly causes infection in patients with chronic underlying illnesses. the pathogenesis of human infection has not been definitively elucidated. bacteremia is the most common clinical manifestation of this infection, although cases of thrombophlebitis, mycotic aneurysm, endocarditis, and pericarditis have also be ...19902193344
isolation, characterization, and host-cell-binding properties of a cytotoxin from campylobacter jejuni.a 68,000-molecular-weight protein was isolated by polyacrylamide gel electrophoresis from the organism-free filtrate of a fully virulent clinical strain of campylobacter jejuni. the eluted protein was heat labile, was inactivated at either ph 3.0 or 9.0, was sensitive to trypsin, and was lethal for fertile chicken eggs. it also had toxic effects on chicken embryo fibroblast, chinese hamster ovary (cho), and intestinal 407 (int407) cells. a monoclonal antibody (cetpmab4) raised to this eluted tox ...19902199497
[occurrence and significance of different campylobacter types in domestic animals in hungary].experiences, including results of original experimental work on campylobacter fetus, c. jejuni and c. coli induced diseases of cattle, sheep, dogs, rabbits poultry and men in hungary are reviewed. out of 31 cases of abortion in cows 29 (93.5%) were causes by c. fetus subsp. venerealis and only one case each (3.2%) by c. fetus subsp. fetus and c. jejuni, respectively. out of the 29 strains of c. fetus subsp. venerealis, 26 belonged to serogroup 01 (a) and only 3 to serogroup 02 (b). campylobacter ...19902209454
surface array protein of campylobacter fetus. cloning and gene structure. 19902229082
campylobacter fetus infection of abdominal aortic aneurysm.a 61-year-old man with campylobacter fetus infection of an abdominal aortic aneurysm treated surgically is presented herein, the fifth survival case reported in the literature. fever and back pain preceded the enlargement of atherosclerotic abdominal aortic aneurysm. the patient tolerated satisfactorily total excision of the aneurysm followed by axillo-femoral prosthetic bypass. antibiotic therapy consisted of intravenous infusion of fosfomycin and gentamicin and oral administration of minocycli ...19902262502
fecal contamination of shanty town toddlers in households with non-corralled poultry, lima, peru.we used direct observer techniques to measure the frequency with which toddler-aged children were contaminated by poultry feces in homes in a peri-urban shanty town in lima, peru. the mean number of fowl was 5.4 (sd 3.1), with 10.0 (sd 10.7) poultry defecations per 12 hours. toddlers' hand contact with poultry feces occurred a mean of 2.9 (sd 3.0) times/12 hours. a mean of 3.9 (sd 4.6) feces-to-mouth episodes per household/12 hours occurred both by direct hand-to-mouth contamination and indirect ...19902297055
control of campylobacter fetus in artificially contaminated bovine semen by incubation with antibiotics before freezing.fresh, diluted semen containing 1.55 x 10(6) cfu/ml of campylobacter fetus subsp. venerealis was incubated with 500 iu of penicillin, 500 micrograms of streptomycin, 160 micrograms of lincomycin and 300 micrograms of spectinomycin per ml at 35 degrees c for 0, 1, 2, 5, 10, 20 or 40 minutes. the semen was cooled to 5 degrees c, packaged in 0.25 ml french straws and then frozen in liquid nitrogen for 2 weeks. immediately after thawing and removal of the antibiotics by centrifugation semen samples ...19902306605
horizontal transmission of campylobacter jejuni amongst broiler chicks: experimental studies.horizontal transmission of campylobacter jejuni was investigated in campylobacter-free broiler chicks. one hundred and twenty chicks housed individually, were provided with water containing 10(2)-10(9) c.f.u./ml c. jejuni. colonization was rapid [47 of 73 (64%) positive cloacal cultures within 3 days and 65 of 73 (89%) within 7 days], dependent on c. jejuni strain and inoculum size but independent of chick age. groups of 5-24 chicks in isolators were exposed to c. jejuni-contaminated water or co ...19902307180
pathogenesis of campylobacter fetus infections. role of surface array proteins in virulence in a mouse model.we developed a mouse model to compare the virulence of campylobacter fetus strains with (s-plus) and without (s-minus) surface array protein (s-protein) capsules. in adult ha/icr mice pretreated with ferric chloride, the ld50 for s-plus strain 84-32 was 43.3 times lower than its spontaneous s-minus mutant 84-54. seven strains of inbred mice were no more susceptible than the outbred strain. in contrast to the findings with salmonella typhimurium by others, 3 x 10(7) cfu of strain 84-32 caused 90% ...19902318963
campylobacter fetus infections in two patients with aids. 19902319151
factors influencing the duration of acute diarrheal disease in infancy.the majority of episodes of acute infectious diarrhea in infancy are of relatively short duration. prolongation of the diarrhea presents problems in management, deterioration of the infant's nutritional status, and an increased mortality. as part of a prospective study of the etiology of acute infectious diarrhea, some factors associated with the probability (more or less) of having self-limiting disease have been identified. the well-nourished infant over 6 months of age is more likely to have ...19902324877
[a case of right-sided infective endocarditis in a drug addict].a 49-year-old man was admitted to mitsui memorial hospital because of fever of unknown origin. since one year ago, he had often used intravenous narcotic drugs. ten months before his admission, he had his first experience of fever and a chill. four months later, he was admitted to a hospital under the diagnosis of pneumonia and he was treated successfully. after his discharge, he began to use drugs again, which resulted in the repetition of fever and chills during four months prior to his admiss ...19902330463
a domestic ferret model of immunity to campylobacter jejuni-induced enteric disease.oral or intravenous inoculation of previously unexposed juvenile and adult ferrets with campylobacter jejuni uniformly resulted in intestinal colonization lasting 2 to 12 days. disease varied from mild to moderate diarrhea, which resolved in 2 to 3 days. orally infected animals developed agglutinin titers of 8 to 256 within 3 weeks, while those infected intravenously developed titers of 256 to 2,048. ferrets which had recovered from campylobacteriosis all developed high titers of agglutinating a ...19902341180
bismuth subsalicylate in the prevention of colonization of infant mice with campylobacter jejuni.infant mice were used for the evaluation of the efficacy of bismuth subsalicylate (bss) in the prevention of the growth of campylobacter jejuni in the intestine. the mic90 of ten c. jejuni strains was 900 micrograms/ml. of three dosage regimens tested, continuous treatment before and after the bacterial challenge, mimicking the way bss is used in the prevention of traveller's diarrhoea, was the most effective. growth inhibition was dose dependent; the high dose of 2000 micrograms per day was mor ...19902347383
serogroups of campylobacter fetus and campylobacter jejuni isolated in cases of ovine abortion.of 38 aborted ovine fetuses from 23 sheep flocks 29 c. fetus subsp. fetus and 22 c. jejuni were isolated and examined biochemically and serologically for heat-stable antigens. serologic examinations were carried out by passive haemagglutination test. in case of c. fetus subsp. fetus strains alkaline antigen extraction was used. antisera to two serogroups of c. fetus and to penner serotype reference strains 1 to 60 were produced in rabbits. abortion was caused in 18 (78.3%) flocks by c. fetus sub ...19902363324
evidence of reinfection with multiple strains of campylobacter jejuni and campylobacter coli in macaca nemestrina housed under hyperendemic conditions.a prospective bacteriologic study of 18 infant pig-tailed macaques (macaca nemestrina) housed in a nursery facility in which campylobacter spp. are endemic was undertaken to determine the epidemiology of infection and reinfection. the isolates of campylobacter jejuni and c. coli cultured from 8 of the 18 infants were characterized by serotyping, dna hybridization, and polyacrylamide gel electrophoresis protein profiles. the chronology of infection was indicative of multiple reinfections with dif ...19902365455
infection of adult syrian hamsters with flagellar variants of campylobacter jejuni.two variants of campylobacter jejuni in1 differing in the expression of flagella, in1 (fla+ mot+, wild type) and in1-nm (fla- mot-), were tested for their ability to establish infection in adult hamsters. animals were challenged intracecally with 2 x 10(9) to 5 x 10(10) cfu and monitored for evidence of infection. none of the challenged animals developed illness. there was a significant difference, however, in the ability of in1 to infect hamsters (35 of 43) compared with that of in1-nm (1 of 42 ...19902365459
partial purification and characterization of the enterotoxin produced by campylobacter jejuni.campylobacter jejuni enterotoxin was partially purified from culture supernatant. the purified fraction after gel filtration indicated three bands at 68, 54, and 43 kilodaltons on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). this fraction enhanced the adenylate cyclase activity of hela cell membranes by 1.5-fold over that of the control. the study with anti-cholera toxin immunoglobulin g (igg) and ganglioside affinity column chromatographies revealed that the eluent from ...19902370099
polynucleotide sequence relationships among flagellin genes of campylobacter jejuni and campylobacter coli.dna probes that encode a complete flagellin gene and various internal regions of the campylobacter coli vc167 flagellin genes were hybridized to 30 strains of c. coli or c. jejuni from 20 different lior serogroups. the results indicated a high overall degree of homology among all of the strains examined. although the most variable regions occurred within the middle of the gene, significant dna homology was observed among many serogroups in this region of the molecule.19902370114
very long term diarrhoea due to campylobacter jejuni. 19902371198
purification, characterization, and localization of a protein antigen shared by thermophilic campylobacters.a protein antigen with an apparent molecular weight (mr) of 31,000 was isolated from 0.2 m glycine hydrochloride (ph 2.2) extracts of a typical human fecal isolate, campylobacter jejuni vc74. the protein was purified to homogeneity on a preparative scale by immunoaffinity chromatography followed by molecular sieving with a superose 12 column. isoelectric focusing under nondenaturing conditions indicated a pi of 9.3, and amino acid composition analysis showed that the protein was unusually rich i ...19902380360
use of an alkaline phosphatase-labeled synthetic oligonucleotide probe for detection of campylobacter jejuni and campylobacter coli.a commercially available synthetic nucleic acid probe (snap) conjugated to alkaline phosphatase was compared with standard culture techniques for detecting campylobacter species. the snap was able to detect either 5 ng of c. jejuni dna or 10(5) cfu of bacteria. the snap could also detect dna extracted from 10(5) cfu in mock-infected stool samples. the snap detected c. jejuni and c. coli but showed no reactivity with c. laridis, c. fetus subsp. fetus, c. fetus subsp. venerealis, c. fennelliae, "c ...19902380381
in vitro susceptibility of campylobacter jejuni to rokitamycin.the susceptibilities of 100 clinical isolates of campylobacter jejuni to rokitamycin, a new macrolide, and eight other oral antibiotics were tested by using a broth microdilution method. rokitamycin demonstrated antibacterial activity that was higher than those of fosfomycin and the tetracyclines and comparable to those of erythromycin and clindamycin, whereas it was slightly less active than the quinolones.19902386373
surface array proteins of campylobacter fetus block lectin-mediated binding to type a lipopolysaccharide.campylobacter fetus strains with type a lipopolysaccharide (lps) and a surface array protein layer (s+) have been found to be pathogenic in humans and animals. spontaneous laboratory mutants that lack surface array proteins (s-) are sensitive to the bactericidal activity of normal human serum. the ability of lectins to determine the presence of the s-layer and differentiate lps type was assessed. we screened 14 lectins and found 3 (wheat germ agglutinin, bandeiraea simplicifolia ii, and helix po ...19902387622
surface array protein of campylobacter fetus. cloning and gene structure.the high molecular mass (97-149 kda) surface array proteins (sap) of campylobacter fetus are critical to virulence. we created a bank of 160,000 random 1.0-6.5-kilobase (kb) chromosomal dna fragments of c. fetus strain 84-32 (23d) using lambda gt11. screening this bank in escherichia coli y1090 with antibody raised against purified sap permitted isolation and purification of a clone with a 4.0-kb insert. subcloning this insert in the e. coli vector, puc9, permitted expression of a protein of app ...19902387868
the development of a transport and enrichment medium for campylobacter fetus.a transport and enrichment medium was developed for campylobacter fetus. from inocula of between 10 and 35 organisms the medium was able to support the multiplication of 19 of 21 strains of c. fetus if the medium was incubated immediately after inoculation; when incubation was delayed for 3 days after inoculation, only seven of the 21 strains multiplied. from inocula of 100-350 organisms all 21 strains multiplied following immediate incubation, and 20 of 21 when incubation was delayed for 3 days ...19902397372
the application of a transport and enrichment medium to the diagnosis of campylobacter fetus infections in bulls.the use of a transport and enrichment medium (tem) in the diagnosis of campylobacter fetus infections in bulls is described. the medium significantly improved the diagnosis rate in samples which, because of the length of time between collection and receipt at the laboratory, were unsuitable for processing by direct culture. the tem was able to support the viability, and subsequent multiplication, of c. fetus in some samples for up to 7 days before the tem was incubated. the submission of paired ...19902397373
natural transformation in campylobacter species.growing cells of campylobacter coli and c. jejuni were naturally transformed by naked dna without the requirement for any special treatment. transformation frequencies for homologous chromosomal dna were approximately 10(-3) transformants per recipient cell in c. coli and 10(-4) in c. jejuni. maximum competence was found in the early log phase of growth. campylobacters preferentially took up their own dna in comparison with escherichia coli chromosomal dna, which was taken up very poorly. three ...19902404960
efficacy of a vaccine to prevent chlamydia- or campylobacter-induced abortions in a sheep flock, chlamydia psittaci, campylobacter fetus, ca jejuni, and salmonella dublin caused abortions. a vaccine that contained c psittaci type i from 2 sources: a cow with pneumonia and an aborted ovine fetus, ca fetus, ca jejuni, and 4 strains of k99 escherichia coli was given to 240 ewes before they were bred. all fetuses, placentas, and lambs, that died within 36 hours of birth were examined for infectious agents. of 55 abortions, 30 (55%) were caused by chlamydia or campylobacter spp ...19902407704
the use of loglinear model to evaluate factors associated with sero-prevalence of campylobacter fetus in dairy cattle.campylobacter fetus is one of the leading infections causing bovine infertility and abortion. in a cross-sectional study, the associations of parity, herd, serological status against leptospira hardjo and haemophilus somnus with the sero-prevalence of campylobacter fetus in dairy cattle were evaluated. four hundred dairy cows from three herds were included in the study. blood samples from the cows were collected in december 1986. an enzyme-linked immunosorbant assay (elisa) was used to determine ...199016726898
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