PMID(sorted descending)
serologic survey for infectious pathogens in free-ranging american bison.from november 1991 through march 1992, we evaluated 101 free-ranging american bison (bison bison) from yellowstone national park, wyoming (usa) for exposure to infectious organisms that commonly infect cattle. no titers were detected for bluetongue virus, bovine leukemia virus, or campylobacter fetus in these 101 bison. detectable antibodies occurred against anaplasma marginale (eight of 76, 11%), bovine respiratory syncytial virus (31 of 101, 31%), bovine viral diarrhea (31 of 101, 31%), bovine ...19979131564
a deletion in the sapa homologue cluster is responsible for the loss of the s-layer in campylobacter fetus strain tk.the surface array protein (sap) of campylobacter fetus strain tk is encoded by seven homologous sapa genes clustered on the chromosomal dna. the spontaneously arising variant strain tk(sap-) produces no sap and carries an approximately 10-kb chromosomal deletion. to elucidate the mechanism underlying the loss of sap synthesis, we analyzed the region containing the sapa homologues and the deletion. we constructed a physical map of the sapa cluster region by aligning the clones that contain sapa h ...19979075619
dna fingerprinting of campylobacter fetus using cloned constructs of ribosomal rna and surface array protein genes.dna fragments coding for the ribosomal rna and the surface array proteins of campylobacter fetus have been cloned from a genomic library constructed in escherichia coli. they were used in the molecular characterization of c. fetus (subsp. fetus; subsp. venerealis) strains by restriction fragment length polymorphism (rflp) method. ribotyping results showed that all strains of the two subspecies can be classified under one ribogroup implying very close relatedness. the sapa gene dna marker, howeve ...19979057261
cloning and nucleotide sequence of the gyra gene from campylobacter fetus subsp. fetus atcc 27374 and characterization of ciprofloxacin-resistant laboratory and clinical isolates.the gyra gene of campylobacter fetus subsp. fetus, which encodes the a subunit of dna gyrase, was cloned, and its nucleotide sequence was determined. an open reading frame of 2,586 nucleotides which encodes a polypeptide of 862 amino acids with an mr of 96,782 was identified. c. fetus subsp. fetus gyra is most closely related to campylobacter jejuni gyra, with 73% homology at the nucleotide level and 78% identity between polypeptides. the next most closely related gyra was that from helicobacter ...19979056011
nested dna inversion as a paradigm of programmed gene rearrangement.programmed gene rearrangements are employed by a variety of microorganisms, including viruses, prokaryotes, and simple eukaryotes, to control gene expression. in most instances in which organisms mediate host evasion by large families of homologous gene cassettes, the mechanism of variation is not thought to involve dna inversion. here we report that campylobacter fetus, a pathogenic gram-negative bacterium, reassorts a single promoter, controlling surface-layer protein expression, and one or mo ...19979023369
purification and characterization of campylobacter rectus surface layer proteins.campylobacter rectus is a putative periodontopathogen which expresses a proteinaceous surface layer (s-layer) external to the outer membrane. s-layers are considered to play a protective role for the microorganism in hostile environments. the s-layer proteins from six different c. rectus strains (five human isolates and a nonhuman primate [nhp] isolate) were isolated, purified, and characterized. the s-layer proteins of these strains varied in molecular mass (ca. 150 to 166 kda) as determined by ...19979009300
[campylobacter fetus meningitis in adults].campylobacter fetus is an uncommon cause of meningitis in the adult. we report a case observed in an 84-year-old man with alcoholic cirrhosis. the patient presented fever, jaundice and a state of mental confusion. blood and cerebrospinal fluid cultures identified campylobacter fetus sensitive to several antibiotics. ciprofloxacine-ceftriaxone combination replaced the antibiotics prescribed empirically prior to identification and led to regression of the fever and normal mental status within 4 da ...19968942943
expression, secretion and antigenic variation of bacterial s-layer proteins.the function of the s-layer, a regularly arranged structure on the outside of numerous bacteria, appears to be different for bacteria living in different environments. almost no similarity exists between the primary sequences of s-proteins, although their amino acid composition is comparable. s-protein production is directed by single or multiple promoters in front of the s-protein gene, yielding stable mrnas. most bacteria secrete s-proteins via the general secretory pathway (gsp). translocatio ...19968898381
biological characterization of campylobacter fetus lipopolysaccharides.lipopolysaccharides (lps) of three strains of campylobacter fetus (subspp. fetus and venerealis, and serotypes a and b), a bacterium of veterinary importance but also a cause of various infections in humans, were assessed for their ability to induce mitogenicity, gelation of limulus amebocyte lysate, lethal toxicity in mice, and pyrogenicity in rabbits. all c. fetus lps exhibited activities lower than those of salmonella typhimurium lps. lps of c. fetus subsp. fetus serotype a had the lowest act ...19968871115
epidemiologic application of pulsed-field gel electrophoresis to an outbreak of campylobacter fetus meningitis in a neonatal intensive care outbreak of nosocomial campylobacter fetus meningitis occurred in a neonatal intensive care unit (nicu). eight c. fetus strains were isolated from 4 infants with meningitis, the mother of the index patient and 2 infants who were asymptomatic intestinal carriers. the pulsed-field gel electrophoresis (pfge) pattern with the restriction endonucleases smal and sall were found to be identical for the nosocomial c. fetus isolates, but the patterns were different from those of sporadic strains. thes ...19968863359
electrophoretic and immunoblot analysis of campylobacter fetus lipopolysaccharides.proteinase k-digested cell lysates from 25 campylobacter fetus subspecies fetus and c. fetus subsp. venerealis strains were examined by sds-page and immunoblotting. three sds-page lipopolysaccharide (lps) profiles were observed. two profiles were consistent with those previously reported for serogroup a and serogroup b and ab isolates and were distinguished by the relative mobility of bands in the o-chain region and by a strong reaction on immunoblots with homologous antisera. the third profile ...19968828127
infective endocarditis caused by campylobacter fetus after allogeneic tooth transplantation: a case report.a patient developed infective endocarditis caused by campylobacter fetus. he gave a history of recent dental extraction and allogeneic tooth transplantation. he was treated with various antibodies to which the organism was said to be sensitive, but it was not until the transplanted tooth was removed that he started to improve. the mode of infection was thought to be blood borne through the open tooth socket from the raw chicken that he ate regularly.19968818256
an environmentally regulated pilus-like appendage involved in campylobacter pathogenesis.examination of strains of campylobacter jejuni, campylobacter coli, and campylobacter fetus by electron microscopy revealed that they produced peritrichous pilus-like appendages when the bacteria were grown in the presence of bile salts. various bile-salt supplements were used and it was found that deoxycholate and chenodeoxycholic acid caused a significant enhancement of pilus production and resulted in a highly aggregative phenotype. morphologically, the pili were between 4 and 7 nm in width a ...19968793884
[acute hepatitis caused by campylobacter fetus]. 19968763076
generation of campylobacter fetus s-layer protein diversity utilizes a single promoter on an invertible dna segment.wild-type strains of campylobacter fetus contain a monomolecular array of surface layer proteins (slps) and vary the antigenicity of the predominant slp expressed. reciprocal recombination events among the eight genomic slp gene cassettes, which encode 97- to 149 kda slps, permit this variation. to explore whether slp expression utilizes a single promoter, we created mutant bacterial strains using insertional mutagenesis by rescue of a marker from plasmids. experimental analysis of the mutants c ...19968730866
the o-specific polysaccharide chain of campylobacter fetus serotype b lipopolysaccharide is a d-rhamnan terminated with 3-o-methyl-d-rhamnose (d-acofriose).an o-specific polysaccharide was liberated from campylobacter fetus subsp. fetus serotype b lipopolysaccharide by mild acid hydrolysis followed by gel chromatography. this polysaccharide was found to contain d-rhamnose and 3-o-methyl-d-rhamnose (d-rha3me, d-acofriose) in a ratio of approximately 24:1, as well as lipopolysaccharide core constituents. the structure of the polysaccharide was studied by 1h-nmr and 13c-nmr spectroscopy, which included two-dimensional cosy, rotating-frame noe spectros ...19968706751
southern blotting analyses of strains of campylobacter fetus using the conserved region of sapa.chromosomal dna of 27 strains of campylobacter fetus was analyzed by southern blotting with a probe of the conserved region of sapa. the probe hybridized with 23 strains that produced type a lipopolysaccharide. these strains had more than six sapa homologs. in southern blots of sali-digested chromosomal dna separated by pulsed-field gel electrophoresis, one fragment from 19 strains and two fragments from 4 strains hybridized. these data indicate that multiple sapa homologs are localized to a lim ...19958588747
campylobacter fetus subspecies fetus septicaemia: sds-page as an aid to speciation.we describe a case of bacteraemia due to an atypical strain of campylobacter fetus ssp. fetus. conventional biochemical and phenotypic characterisation was unhelpful but whole cell protein profiles obtained by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) allowed us to identify this strain.19958586844
[abdominal aortic aneurysm infected with campylobacter fetus spp fetus. report of a case and review of the literature].aortic aneurysm infected with campylobacter fetus spp fetus is rare, the first case having been reported in 1971. we present a case of abdominal aortic aneurysm, with a history of abdominal pain, fever and chills, with identification of this gram negative bacillus in the culture of the aortic wall and visualization of the microorganism in histological examination. surgical correction was performed by interposition of a dracon prosthetic graft. the patient had a good postoperative course, receivi ...19958578095
a detection assay for campylobacter fetus in bovine semen by restriction analysis of pcr amplified dna.a rapid screening assay for campylobacter fetus in bull semen was developed using the polymerase chain reaction (pcr) and restriction endonuclease analysis (rea) to complement isolation by culture. an oligonucleotide primer pair (c1/c2) from the hypervariable region of 16s rrna of c. fetus was used to amplify a 362 base pair fragment by pcr. the pcr/rea assay, which is completed in 10 hours, detected as few as three c. fetus subsp. venerealis cells in experimentally infected raw bull semen and i ...19958540237
bactericidal effects of ozone at nonspermicidal concentrations.a study was conducted to assess the use of ozone (o3) to control pathogens or contaminants of concern to animal breeders and regulatory officials. in separate experiments, samples of fresh bovine semen and pseudomonas aeruginosa, escherichia coli, or campylobacter fetus subsp. venerealis were diluted with antibiotic-free milk (10(6) sperm and 10(6) organisms/ml of diluted semen), exposed in the previous day to a constantly monitored level of 5, 10, 15, or 20 micrograms/ml of o3 for 3-5 min. afte ...19958521350
[a case of campylobacter fetus subspecies fetus meningitis].a 40-year-old male with no history of underlying disease was admitted to hokusho central hospital on may 25, 1991, complaining of high fever and headache. physical examination on admission revealed a temperature of 38.5 degrees c, a pulse rate of 84 beat/min (relative bradycardia) and no abnormal findings for the chest or abdomen. slight neck stiffness without kernig's sign was observed at neurological examination. laboratory data were: esr 11 mm/lh, wbc 12000/mm3, c-reactive protein positive. l ...19938450275
pathogenesis of campylobacter fetus infections: critical role of high-molecular-weight s-layer proteins in virulence.wild-type campylobacter fetus strains possess high-molecular-weight s-layer proteins (s+) and are highly resistant to serum-mediated killing and phagocytosis. spontaneous mutant strains lacking these proteins (s-) are serum and phagocytosis sensitive and have reduced virulence in a mouse model. intact s+ cells were treated with pronase, which made them s- although genotypically s+ and had essentially no effect on other cellular proteins or on viability. treatment with pronase, but not buffer alo ...19938421171
helicobacter acinonyx sp. nov., isolated from cheetahs with gastritis.four strains of a novel helicobacter species were isolated from the stomachs of cheetahs (acinonyx jubilatus) with gastritis. these isolates were phenotypically similar to helicobacter pylori. the isolates were gram-negative, spiral bacteria which grew under microaerophilic conditions at 37 degrees c, but not at 25 or 42 degrees c, and produced urease, catalase, oxidase, alkaline phosphatase, and gamma-glutamyl transpeptidase. the isolates did not ferment glucose, mannitol, inositol, sorbitol, r ...19938379970
[septic campylobacter fetus thrombophlebitis: a new case].the diagnosis of septic thrombophlebitis is difficult and often delayed, but it must be borne in mind in all cases of venous thrombosis accompanied by signs of local and/or systemic infection, or deteriorating under heparin therapy. we report a case of septic thrombophlebitis caused by campylobacter fetus subspecies fetus. the characteristic features, obtained from the literature, of septic thrombophlebitis caused by campylobacter spp are presented.19938362108
production of cytolethal distending toxin (cldt) by campylobacter fetus subsp. fetus isolated from calves.cytolethal distending toxin (cldt) production by campylobacter fetus subsp. fetus isolated from calves was examined using cho cells. twenty-five of the 26 strains tested were positive for cldt with titer of ranging 1:8 to 1:2,048. cldt positive strains were divided into low and high cytotoxin titer groups. isolates from diarrhea cases tended to produce a significant amount of cldt compared with isolates from liver. cldt produced by c. fetus showed no effect on y-1 cells and was heat-labile and t ...19938357933
rearrangement of sapa homologs with conserved and variable regions in campylobacter fetus.the campylobacter fetus surface-layer (s-layer) proteins mediate both complement resistance and antigenic variation in mammalian hosts. wild-type strain 23d possesses the sapa gene, which encodes a 97-kda s-layer protein, and several sapa homologs are present in both wild-type and mutant strains. here we report that a cloned silent gene (sapa1) in c. fetus can express a functional full-length s-layer protein in escherichia coli. analysis of sapa and sapa1 and partial analysis of sapa2 indicate t ...19938346244
prosthetic hip joint infection due to campylobacter fetus.a case of postoperative prosthetic hip joint infection due to campylobacter fetus subsp. fetus is described. difficulties in isolation and antimicrobial susceptibility testing of this organism are discussed.19938308129
septic abortion associated with campylobacter fetus subspecies fetus infection: case report and review of the contrast to the situation in cattle, goat and sheep, campylobacter fetus subspecies fetus only rarely causes disease in humans. while a major inducer of septic abortion in animals, only a minority of clinical infections in humans are found during pregnancy. eleven cases have so far been described in pregnant women. clinical symptomatology is usually mild during gestation but often leads to premature labor. here we present a multigravida with positive cultures for c. fetus who went into septic ...19938300253
campylobacter fetus subspecies fetus peritonitis in continuous ambulatory peritoneal dialysis. 19938290011
the association between antibody titres against campylobacter fetus and milk production efficiency in dairy cattle.the association between serological evidence of exposure to campylobacter fetus and milk production performance was studied in 178 lactating cows from three california dairy herd improvement association herds using a cross-sectional study design in december 1986. elisas were used to determine the antibody titres against campylobacter fetus, haemophilus somnus and leptospira hardjo, which were classified as either negative or positive. the status of a cow as negative or positive against c. fetus ...19938284894
failure of therapeutic vaccination of a bull infected with campylobacter fetus. 19938257318
role of the s-layer proteins of campylobacter fetus in serum-resistance and antigenic variation: a model of bacterial pathogenesis.campylobacter fetus are microaerophilic gram-negative bacteria that are pathogens of animals and humans. these organisms possess paracrystalline surface (s-) layers, composed of acidic high molecular weight proteins. c. fetus strains possessing s-layers are resistant to c3b binding, which explains both serum and phagocytosis-resistance. c. fetus strains also can vary the subunit protein size, crystalline structure, and antigenicity of the s-layer it expresses. therefore, its s-layer permits c. f ...19938238090
granulomatous enteritis and campylobacter bacteremia in a horse.a 10-month-old standard-bred colt was examined because of weight loss, fever, and extensive subcutaneous edema of the ventral aspects of its trunk. diagnosis of granulomatous enteritis was based on d-xylose malabsorption, hypoproteinemia, and rectal mucosal biopsy. campylobacter fetus subsp fetus was cultured from jugular venous blood when the horse was admitted to the hospital and 24 hours later. appropriate antimicrobial agents and glucocorticoids failed to effect clinical remission. after eut ...19938226250
application of enzyme-linked immunosorbent assay for epidemiological studies of diseases of livestock in the tropics of mexico.this study was conducted at the centre for research, teaching and extension in tropical livestock (centro de investigación, enseñanza y extensión en ganadería tropical) of the faculty of veterinary medicine of the national autonomous university of mexico. during the latter part of 1986 and throughout 1988 and 1989, the herd of holstein x zebu cattle at the university was tested for igg antibodies to twenty-one viral, bacterial, rickettsial and parasitic agents. antigens prepared from twenty infe ...19938219327
the association between antibody titres against campylobacter fetus and reproductive efficiency in dairy cattle.the relationship between the antibody titres against campylobacter fetus and various indices of reproductive efficiency was studied in a cross-sectional study of 178 dairy cows from three california dairy herd improvement association herds. blood samples were collected from the lactating cows during december 1986. enzyme-linked immunosorbent assays were used to determine the antibody titres of the cow against campylobacter fetus, haemophilus somnus and leptospira hardjo and were classified as ei ...19938212528
[two cases of campylobacter fetus subsp. fetus sepsis].we encountered two relatively rare cases of sepsis due to campylobacter fetus subsp. fetus (c. fetus). case 1. a 54-year-old female with abdominal polysurgery developed a slight fever and vomiting in august 1984. despite the administration of some digestive drugs by her family doctor, these symptoms continued. in mid-october, she was hospitalized with high fever with chill and rigor on the skin. on the third hospital day, c. fetus was detected in the blood culture. after combination chemotherapy ...19948151152
isolation and immunogenicity of campylobacter fetus subsp. fetus from an abdominal aortic aneurysm.a campylobacter fetus strain was isolated from an abdominal aortic aneurysm in a 56-year-old man who had no significant chronic illness except arteriosclerosis. the strain was identified on the basis of the usual taxonomic markers and fatty acid profiles. the patient's immunological status regarding campylobacter fetus was studied by western blot. the strong immune response observed against the 99 kda protein of the strain confirms that the s-layer protein is the immunodominant antigen of campyl ...19938112355
evaluation of cultural methods and selective media for the isolation of campylobacter fetus subsp venerealis from cattle. 19948080409
septic abortion with intact fetal membranes caused by campylobacter fetus subsp. fetus.we report a case of septic abortion with intact fetal membranes caused by campylobacter fetus subsp. fetus in an 18-year-old woman who was 9 1/2 weeks pregnant.19948077416
biochemical characterization of campylobacter fetus lipopolysaccharides.lipopolysaccharides (lps) of five strains of the human and animal pathogen campylobacter fetus were electrophoretically and chemically characterized. analysis with sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that all the strains produced smooth-form lps with o side chains of relatively constant chain length. upon extraction, lps partitioned into both the water and phenol phases of phenol-water extracts, which showed that two chemical species of lps were present in each c. fe ...19948063409
campylobacter fetus presenting as a septic pleural effusion: a case report.the authors describe a rare case of campylobacter fetus occurring in a patient who showed no overt evidence of immune incompetence. the patient presented with severe cellulitis of the legs, a chronic low grade fever, and a history of recent exposure to sick calves.19948051585
studies on inactivation of pathogenic microorganisms in culture media and in bovine semen by photosensitive agents.the application of three photosensitive agents for disinfection of bovine semen was investigated. bovine microbial pathogens suspended in tissue culture medium and/or pbs and also added to bovine semen were exposed to the photosensitive agents followed by irradiation. hematoporphyrin, hematoporphyrin derivative and thiopyronine were effective against bovine herpes virus-1, bovine viral diarrhoea virus, mycoplasma bovigenitalium, mycoplasma canadense, and ureaplasma diversum in culture media. in ...19948017031
campylobacter fetus endocarditis: two case reports and review.we report two cases of campylobacter fetus endocarditis. the first case involved a bicuspid native aortic valve in a 60-year-old woman, and the second involved a prosthetic aortic valve in a 76-year-old woman. no source of infection was identified in either case. despite antibiotic therapy, hemodynamic deterioration necessitated valve replacement; both patients recovered completely. c. fetus is an uncommon cause of human infection but may be responsible for severe illnesses such as endocarditis ...19948011832
prosthetic hip joint infection due to campylobacter fetus. 19947989567
antimicrobial susceptibility of campylobacter fetus subsp. fetus isolated from blood and synovial fluid.campylobacter fetus subsp. fetus is a rare human pathogen, but can cause serious extraintestinal infections. effective antimicrobial agent is required for the therapy, but we have very limited knowledge on the susceptibility of the organism. in this study, the susceptibility of 25 isolates of the organism to 14 antimicrobial agents was tested by an agar dilution method. antimicrobial agents with low mic ranges, in micrograms/ml, were: meropenem < or = 0.25, dirithromycin < or = 0.5, gentamicin < ...19947975740
persistence of campylobacter fetus subspecies venerealis in experimentally infected heifers. 19947941265
persistence of campylobacter fetus bacteremia associated with absence of opsonizing antibodies.campylobacter fetus causes systemic infections in immunocompromised hosts. we describe a case in which c. fetus bacteremia apparently relapsed after 7 years in a patient with hypogammaglobulinemia and characterize the serum resistance of the patient's c. fetus strain and the inability of the patient's serum, with and without commercial intravenous immunoglobulin, to opsonize this and another c. fetus strain effectively. the probable presence of a sequestered site of infection in bone, the intrin ...19947929763
[campylobacter fetus subsp. fetus in a patient with liver cirrhosis].campylobacter fetus subsp. fetus infections are very rare in adults but can occur in immunocompromised elderly people and in patients with chronic disease. we describe the clinical case of a 52-year-old woman with alcoholic liver cirrhosis, where we isolated c.fetus subsp. fetus from blood culture.19947927478
campylobacter fetus diarrhea in a hutterite colony: epidemiological observations and typing of the causative organism.following a case of campylobacter fetus sepsis and meningitis in a 4-month-old female member of a hutterite colony, an epidemiological investigation revealed at least 18 cases of diarrhea in other members of the colony. c. fetus was isolated from 7 of 15 fecal samples submitted from affected persons. a case control study suggested that persons who worked in the abattoir were 2.03 times more likely to have had diarrhea, but none of the risk factors studied were significant. the epicurve of the ou ...19947910829
a lipopolysaccharide-binding domain of the campylobacter fetus s-layer protein resides within the conserved n terminus of a family of silent and divergent homologs.campylobacter fetus cells can produce multiple s-layer proteins ranging from 97 to 149 kda, with a single form predominating in cultured cells. we have cloned, sequenced, and expressed in escherichia coli a sapa homolog, sapa2, which encodes a full-length 1,109-amino-acid (112-kda) s-layer protein. comparison with the two previously cloned sapa homologs has demonstrated two regions of identity, approximately 70 bp before the open reading frame (orf) and proceeding 550 bp into the orf and immedia ...19957896695
high-frequency s-layer protein variation in campylobacter fetus revealed by sapa mutagenesis.campylobacter fetus utilizes paracrystalline surface (s-) layer proteins that confer complement resistance and that undergo antigenic variation to facilitate persistent mucosal colonization in ungulates. c. fetus possesses multiple homologues of sapa, each of which encode full-length s-layer proteins. disruption of sapa by a gene targeting method (insertion of kanamycin (km) resistance) caused the loss of c. fetus cells bearing full-length s-layer proteins and their replacement by cells bearing ...19947885229
localization of the sapa gene on a physical map of campylobacter fetus chromosomal dna.we constructed a physical map of campylobacter fetus tk(+) chromosomal dna digested by either smai, sali, or noti using pulsed-field gel electrophoresis and southern hybridization data. the genome size of c. fetus tk(+) is 2016kb, larger than that reported by the others. to locate the sapa gene, which encodes the surface array protein (sap), on the physical map, we performed southern hybridizations with probes based on the conserved region of the sapa gene. the results showed that more than seve ...19947872836
[campylobacter fetus, an infrequent microbe, as a cause of spontaneous bacterial peritonitis in cirrhosis].campylobacter fetus has been implicated in the etiology of sepsis and bacteriemias in immunosupressed subjects. in a few cases, it has also been reported to be responsible for spontaneous bacterial peritonitis in cirrhotic patients. we describe the clinical picture of a woman with terminal liver cirrhosis who had bacteriemia and spontaneous bacterial peritonitis caused by this agent. we argue about the history of cleansing enemas and their probable role in the development of the infection. we st ...19947848698
[campylobacter fetus subsp. fetus in a patient with liver cirrhosis]. 19947821945
isolation of campylobacter fetus subspecies fetus from a gluteal abscess. 19947811889
good's syndrome with a block in the early stage of b cell differentiation and complicated by campylobacter fetus sepsis.a 63-year-old man was admitted for campylobacter fetus sepsis and immunodeficiency syndrome with thymoma (good's syndrome). serological examination demonstrated hypoimmunoglobulinemia. analysis of lymphocyte subsets in the peripheral blood and bone marrow showed marked decreases in the proportion of cells bearing b cell markers. however, there were no abnormalities of cellular immunity. this is a rare case of good's syndrome in japan in which the pathogenic mechanism involved a block in the earl ...19947803919
segmental conservation of sapa sequences in type b campylobacter fetus cells.campylobacter fetus cells may exist as either of two defined serogroups (type a or b) based on their lipopolysaccharide (lps) composition. wild-type strains contain surface array proteins (s-layer proteins) that have partial antigenic cross-reactivity but bind exclusively to lps from homologous (type a or b) cells. type a cells possess 8 homologs of sapa, which encodes a 97-kda s-layer protein; the gene products of these homologs have a conserved n terminus of 184 amino acids. to further explore ...19957797493
[campylobacter fetus subspecies fetus endoaortitis on a bentall tube prosthesis. apropos of a case].campylobacter fetus is a rare cause of endocarditis and endoaortitis: the authors believe this to be the second reported case of infection of an intracardiac prosthesis. the patient was a man who had already undergone replacement of the aortic valve and ascending aorta, and a gastrectomy, which were predisposing factors. the portal of entry was not found. the diagnosis was confirmed by positive blood cultures and transoesophageal echocardiography. the outcome was rapidly fatal despite antibiotic ...19947771897
interleukin-8 response of gastric epithelial cell lines to helicobacter pylori stimulation in vitro.gastric infection with helicobacter pylori activates a mucosal inflammatory response by mononuclear cells and neutrophils that includes expression of cytokines interleukin-1 beta (il-1 beta), il-6, tumor necrosis factor alpha, and il-8. in this study, we analyzed the il-8 response of human gastric cancer cell lines (kato iii, ags, and mkn28) to h. pylori infection in vitro. il-8 mrna expression was detected by reverse transcription-pcr amplification of rna extracted from epithelial cells after i ...19957729872
protein shift and antigenic variation in the s-layer of campylobacter fetus subsp. venerealis during bovine infection accompanied by genomic rearrangement of sapa homologs.campylobacter fetus subsp. venerealis isolated from a case of human vaginosis was inoculated into the uterus of a c. fetus-negative heifer. isolates obtained weekly from the vaginal mucus exhibited variations in high-molecular-mass-protein profiles from that of the original inoculum, which had a dominant 110-kda s-layer protein. immunoblots of the weekly isolates with monoclonal antibody probes against the 110-kda s-layer protein and other c. fetus s-layer proteins demonstrated antigenic shifts. ...19957721688
shift in s-layer protein expression responsible for antigenic variation in campylobacter fetus.campylobacter fetus strains possess regular paracrystalline surface layers (s-layers) composed of high-molecular-weight proteins and can change the size and crystalline structure of the predominant protein expressed. polyclonal antisera demonstrate antigenic cross-reactivity among these proteins but suggest differences in epitopes. monoclonal antibodies to the 97-kda s-layer protein of campylobacter fetus subsp. fetus strain 82-40lp showed three different reactivities. monoclonal antibody 1d1 re ...19937688715
a survey of campylobacter bacteremia in three danish counties, 1989 to 1994.over a 5-year period (april 1989 to march 1994) bacteremia caused by campylobacter species was diagnosed in 15 patients in a population of 1.3 million in three counties in jutland province, denmark. the incidence was 0.2/100,000/year and the ratio of blood to fecal isolates, was 0.008. campylobacter coli and campylobacter jejuni accounted for 6 cases each, campylobacter fetus for 2, and in 1 case the species diagnosis was uncertain. seven patients were males and 8 females; median age was 42 (ran ...19957660078
analysis of strains of campylobacter fetus by pulsed-field gel electrophoresis.campylobacter fetus chromosomal dna from 21 strains was analyzed by pulsed-field gel electrophoresis. the fingerprint patterns generated with smai and sali were distinctive. using the profiles obtained by pulsed-field gel electrophoresis, we established the phylogenetic dendrogram of c. fetus to identify the genetic relationship of the strains.19957650215
iatrogenic compressive lumbar myelopathy and radiculopathy in adult cattle following injection of an adjuvanted bacterin into loin muscle: histopathology and ultrastructure.compressive lumbar myelopathy is a recognized iatrogenic complication of injecting water-in-oil vaccines into paravertebral sites of laboratory animals and chickens. herein, we report the histologic and ultrastructural features of a similar complication in a herd of cattle. iatrogenic posterior paresis developed over 34 days in 56 of 610 cows (9.2%) following injection of a commercial bacterin 11-34 days earlier into m. longissimus lumborum. the bacterin was composed of inactivated escherichia c ...19957619907
in vivo pefloxacin-resistant campylobacter fetus responsible for gastro-intestinal infection and bacteremia associated with arthritis of the hip.the authors report a case of campylobacter fetus subsp. fetus gastro-intestinal infection and bacteremia with poly-arthritis, mainly of the hip, in a french patient simultaneously suffering from cirrhosis of the liver. the outcome was eventually favorable, however only after a trial of ineffective pefloxacin-gentamicin therapy. the authors suggest: (i) gentamicin should not be given alone in c. fetus subsp. fetus infections, and (ii) pefloxacin should not be given if antibiotic sensitivities dat ...19957618366
genome map of campylobacter fetus subsp. fetus atcc 27374.a physical map of the chromosome of campylobacter fetus subsp. fetus was constructed by using pulsed-field gel electrophoresis of restriction fragments generated by sali, smai and noti. digestion of the type strain atcc 27374 with these restriction endonucleases resulted in generating 4-14 fragments. the order of the fragments was deduced from hybridization of these restriction fragments to southern blots of pulsed-field gel electrophoresis gels generated by the other two enzymes. the estimated ...19957590179
promising new carbapenem antibiotics for treatment of neonatal meningitis due to campylobacter fetus. 19957561257
identification of campylobacter fetus by pcr-dna probe method.a pcr method for rapid identification of campylobacter fetus subsp. fetus was evaluated. a fragment of the gene coding for 16s rrna was amplified from crude cell lysates of 18 c. fetus strains and 30 strains representing other campylobacter species and subspecies. the amplicons were probed by dot blot hybridization with a digoxigenin-labeled c. fetus-specific oligonucleotide probe. the probe reacted only with c. fetus subsp. fetus and c. fetus subsp. venerealis and may be useful for rapid identi ...19957542273
serum antibody response to the superficial and released components of helicobacter pylori.superficial and released components were extracted from six selected helicobacter pylori strains. the protein and antigenic profiles of these extracts were representative of the profiles found most frequently among the clinical strains and included major peptidic fractions at 19, 23.5, 57, 68, 76, 118, and 132 kda and major antigens at 68, 57, and 23.5 kda. immuno-cross-reactions were seen with a hyperimmune rabbit serum to campylobacter fetus but not with sera to campylobacter jejuni or salmone ...19947496968
campylobacter fetus ssp jejuni: isolation from patients with gastroenteritis.within a seven-month period, campylobacter fetus ssp jejuni was isolated in east tennessee from 18 patients with gastroenteritis; 83% of these patients had bloody diarrhea. absence of other enteric organisms such as salmonella, shigella, and yersinia implicated c fetus ssp jejuni as the causative agent. a fourfold increase in titer by tube agglutination from four of eight patients studied supported the pathogenicity of this organism. treatment with erythromycin alleviated gastroenteritis symptom ...19817466431
'1001' campylobacters: cultural characteristics of intestinal campylobacters from man and animals.the cultural characteristics of 1220 campylobacter strains from a variety of sources are described. forty-two were identified as campylobacter fetus ssp. fetus (véron & chatelain, 1973), 1120 as members of the c. jejuni/c. coli group, and 58 did not conform to any known description. sixteen of the latter strains had the basic characteristics of c. fetus but were atypical in certain other respects. the other 42 strains had the thermophilic characteristics of the jejuni/coli group, but were resist ...19807462593
campylobacter enteritis in order to establish a reasonable protocol for a diagnostic laboratory we conducted a survey during which we confined the routine culture of stool samples for campylobacter fetus to two groups--all infants under 2 years of age, and older children and adults with obviously diarrhoeic stools. camp. fetus was isolated from 100 of 2323 stool specimens (4,3%). this is within the 3 - 8% isolation rates previously reported from surveys in which all specimens were cultured. camp. fetus isolates represe ...19817455853
laboratory-acquired campylobacter enteritis.a case of gastroenteritis occurred in a laboratory worker 2 1/2 days after exposure in the laboratory to a culture of campylobacter fetus ss jejuni. spontaneous recovery was complete after 1 1/2 weeks.19817455753
production of diarrhoea and dysentery in experimental calves by feeding pure cultures of campylobacter fetus subspecies jejuni.pure cultures of campylobacter fetus subspecies jejuni of bovine origin were used to inoculate three ruminating calves in each of two experiments and three milk-fed calves in another. inoculated animals all developed clinical signs which included fewer (to 41 degrees c), diarrhoea and sporadic dysentery within one to three days of inoculation. diarrhoeic faeces were dark, mucoid, of uniform consistency and were passed for six to 15 days. the eight control animals did not develop this syndrome. c ...19807445343
hypocomplementemia and campylobacter fetus infection.a middle-aged alcoholic man had low c3 levels during two consecutive episodes of bacteremia with campylobacter fetus, with a return of c3 levels to normal during the interbacteremic period. total complement levels remained below normal throughout the patient's illness, whereas c4 and c3 activator values were in the normal or supranormal range. a rise and fall in hemagglutinating antibody titers, documenting an immune response, coupled with reasonable antimicrobial therapy were not sufficient to ...19807444533
campylobacter fetus subspecies jejuni: a common cause of diarrhea in sweden.stool samples from approximately 2,550 patients with gastrointestinal infections were cultured for bacterial pathogens between january 1978 and september 1979, and 277 campylobacter (10.9%), 183 salmonella, 89 shigella, and 17 yersinia infections were identified. campylobacter was found in all age groups, with the majority in the age group of 20-34 years. most cases of campylobacter diarrhea were isolated during summer or late fall. after five weeks, 90% of the patients had no campylobacter in t ...19807441005
campylobacter spp in enteric lesions in cattle.campylobacters were isolated from the intestinal mucosa of 16 of 47 cattle examined post mortem. of the 17 isolates seven were identified as campylobacter fetus subsp jejuni, eight as cf subsp intestinalis, one as c fecalis and one remained unidentified. cf subsp jejuni and cf subsp intestinalis were both recovered from one case. gross changes were not always seen at the sites from which campylobacters were isolated but microscopic changes were seen in all cases. the changes seen are described a ...19807434541
an outbreak of acute enteritis due to campylobacter fetus subspecies jejuni at a nursery school of outbreak of acute enteritis due to campylobacter fetus subspecies jejuni involving a total of 35 out of 74 children occurred at a nursery school of tokyo in january 1979 and lasted for 7 days. it was the first case of a community outbreak of the disease to be recognized in japan. the major symptoms observed in the patients consisted of diarrhea (88%), fever (82%), abdominal pain (39%), and vomiting (6.1%). the rate of isolation of the organism from the patients was 39%. sera of four patients ...19807432196
[campylobacter fetus subspecies jejuni in chickens (author's transl)].a bacteriological examination of 239 chickens coming from 89 commerical flocks yielded a high incidence of campylobacter fetus subspecies jejuni isolations. from 30.1% of the birds or 41.6% of the flocks this micro-organism was isolated from 28.6% of the jejunal contents and 11.2% of the bile samples respectively. in contrast this agent could not be isolated from the skin or peritoneal cavity of 750 carcasses of broilers (13 flocks) before of after freezing in. no correlation could be found betw ...19807423469
campylobacter fetus subsp. jejuni as a cause of gastroenteritis in jakarta, indonesia.campylobacter fetus subsp. jejuni was isolated from the feces of 15 out of 144 (10%) children (0 to 9 years old) and 4 out of 251 (2%) adults with gastroenteritis and was found together with another enteric pathogen in 2 of the children and in all 4 adults. it was isolated from 2 out of 7 (28%) children and 3 out of 160 (2%) adults with suspected typhoid fever. the bacterium was recovered from 3 out of 4 orphanage children with diarrhea and from 1 without symptoms and was isolated from only 1 ch ...19807419706
bacteriostatic and bactericidal activities of 24 antimicrobial agents against campylobacter fetus subsp. jejuni.the bacteriostatic and bactericidal activities of 24 antimicrobial agents were tested with the dynatech mic 2000 system against 86 strains of campylobacter fetus subsp. jejuni from human sources. the penicillins (penicillin g, ampicillin, amoxycillin, carbenicillin) had poor activity. ampicillin and amoxycillin were equally active. cefotaxime revealed a rather good activity. erythromycin, gentamicin, tobramycin, amikacin, and furazolidone were the most active compounds. two strains (2.3%) were r ...19807416738
serology of campylobacter fetus ss. jejuni )"related" campylobacters). demonstration of strain-specific and interstrain-related antigens by immunoelectrophoresis and co-agglutination.rabbit antisera against two strains of campylobacter fetus ss. fetus (serotype a), two strains of c. fetus ss. intestinalis (serotypes a and b respectively), and eight stains of c. fetus ss. jejuni were used in serological studies of these strains with the use of co-agglutination (coa), line-rocket immunoelectrophoresis (l-rie) and rocket-line immunoelectrophoresis (r-lie). whole bacterial cells, either heated at 56 degrees c. boiled or atuoclaved, were used in coa tests. unheated sonicates were ...19807415842
antigens of campylobacter fetus subsp fetus eliciting vaccinal immunity in heifers.these studies were designed to identify the antigens of campylobacter fetus subsp fetus that elicit vaccinal immunity. heifers were vaccinated subcutaneously twice (42 days between vaccinations) with bacterins containing k, o, and h antigens (group a), o and h antigens (group b), or o antigen (group c) of the challenge strain. five heifers were not vaccinated and served as controls (group d). two weeks after heifers were given the second vaccination, the immunity of all heifers was challenge exp ...19807406293
campylobacter fetus subspecies jejuni bacteremia. 19807403927
a human strain of campylobacter fetus subsp. intestinalis grown at 42 degrees c. 19807400364
a human strain of campylobacter fetus subsp. intestinalis grown at 42 degrees c. 19807400351
campylobacteriosis in jamaican cattle.infertility is a widespread problem among cattle herds in jamaica and an intergovernmental project was initiated to investigate the problem. one aspect of the project included diagnosis of campylobacteriosis (vibriosis), which was suspected in some herds but had not been confirmed in the laboratory. tests were undertaken in late 1978 using improved techniques for handling and transporting specimens to the laboratory. campylobacter fetus subspecies fetus was isolated from five of six herds tested ...19807395025
a case of campylobacter fetus septicemia in maine. 19807391695
pathogenicity of campylobacter fetus subsp. venerealis in causing infertility in cattle. 19807388595
meningitis caused by campylobacter fetus ssp jejuni. 19807388445
broiler chickens as potential source of campylobacter infections in humans.of 46 broiler chickens from a live poultry market in new york city, 38 (83%) harbored campylobacter fetus subsp. jejuni in their rectal flora. the observed mean number of c. fetus per g of feces was 4.4 x 10(6). the organisms survived in the feces for at least 96 h at 4 degrees c whether stored in the gut or transferred to a vial. the best survival medium for pure cultures of c. fetus subsp.jejuni was heart infusion broth supplemented with sterile blood and kept in a microaerophilic atmosphere.19807381018
cellular fatty acid composition of campylobacter fetus.the cellular fatty acid composition of 29 human and animal isolates of campylobacter fetus was determined with gas-liquid chromatography. all strains contained small amounts of 3-hydroxytetradecanoic acid, suggesting the presence of lipid a. each of 13 different blood or stool isolates of c. fetus subsp. jejuni obtained from humans or fowl contained a 19-carbon cyclopropane acid which was not present in c. fetus subsp. fetus (6 strains from cattle) or c. fetus subsp. intestinalis (10 strains fro ...19807381008
campylobacter colitis presentin as inflammatory bowel disease with segmental colonic ulcerations.campylobacter fetus subspecies have recently been recognized as an important cause of diarrheal illness in humans. we report a case of culture positive c. fetus colitis with colonoscopic findings of segmental mucosal edema, loss of normal vascular pattern with ulceration, and patchy involvement of mucosa. these colonoscopic findings were similar to those seen in granulomatous colitis. however, these findings may be difficult to distinguish from a number of other colonic mucosal diseases such as ...19807380209
mucosal invasion in campylobacter enteritis.campylobacter fetus subspecies jejuni has recently been recognized as a significant cause of enteritis in humans. the organism has been previously postulated to cause illness by direct invasion of the mucosa of the gastrointestinal tract, but this has not been documented. reported is a case of campylobacter enteritis in which mucosal ulceration and crypt sbscesses were seen on rectal biopsy, suggesting that the pathogenesis of campylobacter fetus enteritis involves direct mucosal invasion.19807377140
reservoirs for human campylobacteriosis.the epidemiology of human enteric infection due to campylobacter fetus subspecies jejuni is not well understood. to determine whether an endogenous human reservoir is present, the rate of isolation of c. fetus subspecies jejuni from several populations of diarrheal and asymptomatic adults and children were compared. c. fetus subspecies jejuni was recovered from 4.1% of patients with diarrhea and 66.7% of household contacts of the index patients who themselves had diarrhea. carriage of organisms ...19807373089
hippurate hydrolysis by campylobacter additional method for differentiating between campylobacter fetus subsp. jejuni and c. fetus subsp. intestinalis is reported. strains of c. fetus subsp. jejuni (18/20) were shown to hydrolyze hippurate in the 2-h rapid test, whereas strains of c. fetus subsp. intestinalis did not.19807372805
[the presence of campylobacter fetus subspecies jejuni in normal slaughtered pigs (author's transl)].as part of epidemiological studies the intestinal contents of 300 normal slaughtered pigs were examined for the presence of c. fetus jejuni. samples were collected from six different slaughter houses. from 182 of these samples (60.7 per cent) c. fetus spp. jejuni was isolated using the method as described by lauwers (8). isolates were confirmed by the results of the following tests: oxidase, catalase, motility, growth at 25 degrees c, production of h2s, reduction of nitrate, growth in 3.5 per ce ...19807352330
acute colitis caused by campylobacter fetus ss. jejuni.campylobacter fetus ss. jejuni has been recently recognized as a common human enteric pathogen; however, the pathology and pathophysiology of the enteritis caused by this agent are still largely obscure. we report 4 patients who presented with acute abdominal pain and bloody diarrhea and who had c. fetus ss. jejuni isolated from fecal cultures. each patient showed a fourfold rise in serum igg titer to the organism isolated. in all 4 patients colonic involvement was noted on sigmoidoscopic examin ...19807351284
[campylobacter fetus jejuni: incidence in diarrhea and antimicrobial sensitivity]. 19807348345
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