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characterization of campylobacter jejuni and campylobacter coli isolated from waste water.the raw sewage samples of the investigated municipal sewage plant (sewage flow of about 30,000 m3/d) contained about 10(3) campylobacters/100 ml. the sewage plant reduced 80.8% of organic wastes (cod), 85.0% of colony counts (20 degrees c), 92.5% of total coliforms and 88.0% of campylobacters. the sewage plant effluent contained an average of 1.3 x 10(2) campylobacters/100 ml. about 10(10) campylobacters are discharged with the effluent of the high-output sewage plant into the receiving water ev ...19883057769
adherence of campylobacter jejuni and campylobacter coli to porcine intestinal brush border membranes.the adhesion of campylobacter jejuni and c. coli to isolated porcine intestinal brush border membranes was studied by phase-contrast and electron microscopy. approximately 45% of the cell population adhered to the brush borders, possibly in a specific manner. pretreatment of the brush borders with trypsin or pronase, and competitive inhibition with l-rhamnose caused a slight reduction of the adhesion. different forms of pretreatment of the bacterial cells reduced their ability to adhere, but als ...19883046640
nucleotide sequence of the kanamycin resistance determinant of the pneumococcal transposon tn1545: evolutionary relationships and transcriptional analysis of apha-3 genes.the nucleotide sequence of the kanamycin resistance determinant apha-3 encoded by transposon tn1545 from streptococcus pneumoniae was determined and compared to those of plasmids pjh1 and pip1433 from streptococcus faecalis and campylobacter coli, respectively. the three sequences were found to be identical and differed by two substitutions and the deletion of a codon from that of plasmid psh2 from staphylococcus aureus. comparison of the 5' noncoding sequences indicated that the regions contain ...19873039302
antimicrobial resistance of campylobacter jejuni and campylobacter coli with special reference to plasmid profiles of japanese clinical isolates.a total of 111 clinical isolates of campylobacter jejuni and 10 clinical isolates of campylobacter coli were characterized by their susceptibility to nine antimicrobial agents and by their plasmid profiles on agarose gel electrophoresis. all of the c. jejuni isolates were susceptible to chloramphenicol, ciprofloxacin, erythromycin, kanamycin, and nalidixic acid, but 55% were tetracycline resistant. in the 10 c. coli isolates, a high prevalence of multiple-antibiotic resistance was noted. plasmid ...19873038005
evolution and transfer of aminoglycoside resistance genes under natural conditions.3'-aminoglycoside phosphotransferases [aph(3')] were chosen as a model to study the evolution and the transfer of aminoglycoside resistance genes under natural conditions. comparison of the amino acid sequences of aph(3') enzymes from transposons tn903 (type i) and tn5 (type ii) detected in gram-negative bacteria, from the gram-positive staphylococcus and streptococcus (type iii), from the butirosin-producing bacillus circulans (type iv) and from a neomycin-producing streptomyces fradiae (type v ...19863027020
aminoglycoside-phosphotransferases aph(3')-iv and aph(3") synthesized by a strain of campylobacter coli.campylobacter coli strain 981 of animal origin was resistant to erythromycin, tetracycline, streptomycin, kanamycin, ribostamycin, neomycin, paromomycin, lividomycin, and butirosin. resistance to aminoglycosides of strain 981 was mediated by phosphotransferases aph (3') type-iv and aph (3"). c. coli 981 harboured three plasmids of 24, 34, and 40 megadaltons respectively. none of these plasmids were transferable to escherichia coli k-12 by conjugation.19863019983
cytotoxic and cytotonic factors produced by campylobacter jejuni, campylobacter coli, and campylobacter laridis.complete toxigenicity studies were performed on 341 strains of campylobacter spp., including 23 nonhuman isolates. toxin profiles based on both cytotonic and cytotoxic factors were determined after analyzing responses in vero, hela, cho and y-1 cells. suckling mouse assays were consistently negative for all culture filtrates tested. toxin-producing strains were frequently encountered among both the human and nonhuman strains of campylobacter jejuni, c. coli, and c. laridis investigated. strains ...19863018039
structural relationship between the genes encoding 3'-aminoglycoside phosphotransferases in campylobacter and in gram-positive cocci.campylobacter coli strain bm2509 resistant to ampicillin, chloramphenicol, erythromycin, kanamycin, spectinomycin, streptomycin and tetracycline was isolated from a patient with hospital-acquired diarrhoea. resistance to kanamycin has not been thus far described in campylobacter. phosphocellulose paper-binding assay indicated that resistance to kanamycin and structurally related antibiotics in strain bm2509 was due to synthesis of a 3'-aminoglycoside phosphotransferase of type iii (aph(3')-iii), ...19853002237
survey of plasmids and resistance factors in campylobacter jejuni and campylobacter coli.a total of 688 isolates of campylobacter jejuni and campylobacter coli were screened for the presence of plasmid dna by agarose gel electrophoresis and were tested for susceptibility to ampicillin, chloramphenicol, erythromycin, streptomycin, and tetracycline. of the isolates examined, 32% were noted to harbor plasmid dna, ranging in size from 2.0 to 162 kilobases. only tetracycline resistance was noted to correlate with the presence of plasmids. plasmids capable of transferring tetracycline res ...19852984981
comparative in vitro activity of a-56268 (te-031) against gram-positive and gram-negative bacteria and chlamydia trachomatis.the in vitro activity of a-56268 (te-031) was determined and compared with that of 13 antibiotics against 401 gram-positive and gram-negative bacteria and 11 strains of chlamydia trachomatis. a-56268 was very active against methicillin-susceptible staphylococcus aureus and neisseria gonorrhoeae, and was among the most active of the agents tested against listeria monocytogenes, streptococci and chlamydia trachomatis. it was moderately active against haemophilus spp., vibrio spp., campylobacter je ...19872954818
reproduction of proliferative enteritis in gnotobiotic pigs.gnotobiotic pigs dosed orally with filtrates (0.8 and 0.65 micron) of intestinal mucosa from a pig affected by proliferative haemorrhagic enteropathy developed lesions of proliferative enteritis, affecting mainly the ilea. other piglets dosed with filtrates of affected mucosa from the same source and from other proliferative haemorrhagic enteropathy or intestinal adenomatosis mucosae, did not develop lesions. all inocula contained numerous campylobacter-like organisms evident in stained smears, ...19892922502
campylobacter jejuni and campylobacter coli diarrhoea in rural and urban populations in yugoslavia.during a 4-month period during the summer of 1985, campylobacters were isolated from 338 (16.3%) of 2080 patients with acute diarrhoea attending the university hospital of infectious diseases in zagreb. of these isolates 220 (64.1%) were campylobacter jejuni and 118 (34.9%) were c. coli. the patients were drawn from three residential zones in and around zagreb: inner city, peripheral city and rural. incidences of campylobacter diarrhoea ranged from 71 per 100,000 per year in inner city residents ...19892917618
a note on adhesion of bacteria to chicken muscle connective tissue.laboratory cultures of bacteria were tested for the ability to attach to collagen fibres of intact chicken muscle connective tissue. all salmonellas, fimbriate strains of escherichia coli and a strain of campylobacter coli were able to attach to tissue only when suspended in distilled water. prior immersion of tissue in sterile water for 20 min or extended immersion in these bacterial suspensions was a prerequisite for adhesion. attachment could be prevented by the addition of physiological leve ...19872888744
raw beef, pork and chicken in japan contaminated with salmonella sp., campylobacter sp., yersinia enterocolitica, and clostridium perfringens--a comparative study.one hundred and twenty samples each of raw ground beef, pork and chicken from ten local grocery stores in shimane prefecture, japan, were examined for the presence of salmonella sp. (sal), campylobacter jejuni (cj), campylobacter coli (cc), yersinia enterocolitica (ye), and clostridium perfringens (cp) from april 1984 to march 1985. a total of 205 isolates of sal (112 strains), cj (64 strains), cc (one strain), ye (7 strains) and cp (21 strains) were recovered from 17 beef (14.2%), 31 pork (25.8 ...19872887078
substrate utilization by campylobacter jejuni and campylobacter coli.an attempt was made to elucidate in campylobacter spp. some of the physiologic characteristics that are reflected in the kinetics of co2 formation from four 14c-labeled substrates. campylobacter jejuni and c. coli were grown in a biphasic medium, and highly motile spiral cells were harvested at 12 h. of the media evaluated for use in the metabolic tests, minimal essential medium without glutamine, diluted with an equal volume of potassium sodium phosphate buffer (ph 7.2), provided the greatest s ...19862877615
restriction endonuclease digest patterns of chromosomal dna from nitrate-negative campylobacter jejuni-like organisms.high molecular weight chromosomal dna was isolated from ten nitrate-negative campylobacters of human origin (gastric biopsies and faecal specimens). the dna was digested with various restriction endonucleases and the patterns obtained were compared with those of campylobacter coli, c. fetus subsp. fetus, c. jejuni and c. laridis reference strains. electrophoresis of hae iii digests of dna in 0.5% agarose gave excellent patterns which comprised at least 25 well-resolved bands with fragment sizes ...19852876913
genetic characterization of kanamycin resistance in campylobacter coli.the restriction map of plasmid pip1433 from campylobacter coli bm2509, isolated in france, was constructed and the location of the kanamycin (km) and tetracycline (tc) resistance markers determined using probes for apha-3 and teto genes, respectively. these dna probes were used to study a second km- and tc-resistant strain c. coli ua696, isolated in canada. in this strain, the km resistance determinant was located in the chromosome. like pip1433, the km resistance determinant in c. coli ua696 sp ...19882855308
nucleotide sequence analysis of tetracycline resistance gene teto from streptococcus mutans dl5.streptococcus mutans dl5, isolated from the dental plaque of a pig, was resistant to high levels of streptomycin (sm, 20 mg/ml), erythromycin (em, 1 mg/ml), and tetracycline (tc, greater than 100 micrograms/ml), but contained no detectable plasmid dna. the smr and emr determinants were cloned from cellular dna on the self-replicating 5-kilobase-pair (kbp) ecori fragment of pam beta 1 and the 4.2-kbp cryptic plasmid pva380-1, respectively, by transformation of streptococcus sanguis challis. helpe ...19882841293
the relationship between intestinal campylobacter species isolated from animals and humans as determined by brenda.intestinal thermophilic campylobacter species produce stable patterns when subjected to bacterial restriction endonuclease dna analysis (brenda); this technique is therefore of considerable value in epidemiological studies. brenda was used to examine thermophilic campylobacter species from humans, wild and domestic animals. one hundred and ninety-four (61%) of 316 isolates of campylobacter jejuni from humans had brenda patterns which could be matched to those of animal isolates. poultry appear t ...19882837404
gene disruption and replacement as a feasible approach for mutagenesis of campylobacter jejuni.campylobacter jejuni and campylobacter coli are important causes of human enteric infections. several determinants of pathogenicity have been proposed based on the clinical features of diarrheal disease and on the phenotypic properties of campylobacter strains. to facilitate an understanding of the genetic determinants of campylobacter virulence, we have developed a method for constructing c. jejuni mutants by shuttle mutagenesis. in the example described here, a kanamycin resistance gene was in ...19882832375
genomic rearrangements associated with antigenic variation in campylobacter coli.campylobacter coli and campylobacter jejuni share a limited number of highly conserved dna sequences with members of the family enterobacteriaceae. one of these sequences was cloned from c. coli vc167, and the region of homology to the enteric sequences was determined to be confined to a 700-base-pair region. the dna represented in this clone undergoes a programmed, reversible rearrangement in vc167 that is associated with flagellar antigenic variation.19882826396
gene transfer from escherichia coli to campylobacter species: development of shuttle vectors for genetic analysis of campylobacter jejuni.a shuttle cloning vector (pil550) has been constructed which can be mobilized from escherichia coli to campylobacter jejuni, campylobacter coli, and campylobacter fetus by complementation with the transfer functions of an incp plasmid in trans, with a frequency of 10(-4) transconjugants per donor. we also present evidence for a dna modification system in c. jejuni.19872822671
comparative in-vitro activity of meropenem on clinical isolates from the united kingdom.mics of meropenem were determined for a wide range of common bacteria of clinical importance. for enterobacteriaceae, aeromonas spp., haemophilus influenzae, branhamella catarrhalis, neisseria gonorrhoeae, gardnerella vaginalis, campylobacter coli/jejuni, beta-haemolytic streptococci and anaerobes other than clostridium difficile, mics were almost always within the range 0.002-0.5 mg/l. the activity of meropenem for these organisms was always greater than that of imipenem and piperacillin, and w ...19892808214
structural and antigenic properties of campylobacter coli lipopolysaccharides.lipopolysaccharides (lpss) from o-serotype reference strains and from serotyped isolates of campylobacter coli were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in combination with silver staining and immunoblotting to elucidate the molecular basis for the thermostable antigenic diversity in c. coli. electrophoresed low-mr lps was detectable by silver staining and immunoblotting, but high-mr lps was demonstrable only by immunoblotting. eight different antigenic specifici ...19892807533
in vitro activities of lomefloxacin and temafloxacin against pathogens causing diarrhea.the in vitro activities of temafloxacin (a63004) and lomefloxacin (sc-47111; ny-198) were compared with those of seven other antibiotics against 146 isolates of bacterial enteric pathogens, including campylobacter jejuni and campylobacter coli. ciprofloxacin was the most active drug against the salmonella, shigella, yersinia, and vibrio spp. tested. lomefloxacin, temafloxacin, and difloxacin were the most active drugs tested against campylobacter spp. (mic for 90% of strains, 0.125 to 0.25 micro ...19892802563
interaction of campylobacter species with antibody, complement and phagocytes.the opsonisation of four different campylobacter species for human neutrophils was studied using a chemiluminescence system and electron microscopy. opsonisation of campylobacter fetus, campylobacter coli, and campylobacter jejuni was mediated by antibody and enhanced by complement. antibody was not, however, required for the phagocytosis of campylobacter pylori because it activates the classical pathway of complement directly. this unusual property may be important in the pathogenesis of c pylo ...19892788111
enterotoxin production and serogroups of campylobacter jejuni and campylobacter coli from patients with diarrhea and from healthy laying hens.enterotoxin production, a possible virulence factor, was determined in campylobacter jejuni and campylobacter coli by two different techniques, the cho cell test and the gm1 enzyme-linked immunosorbent assay. the frequency of enterotoxigenic campylobacter strains was 32% in strains from both humans with acute enteritis and healthy laying hens, as measured by the cho cell test. the cho cell test was significantly more sensitive than the gm1 enzyme-linked immunosorbent assay in the detection of en ...19892754001
identification of campylobacter jejuni and c. coli by gel electrophoresis of the outer membrane proteins.analysis of the electrophoretic profiles of the outer membrane proteins could be used to differentiate campylobacter jejuni (16 strains) from campylobacter coli (10 strains). this observation was confirmed by the study of dna homology obtained by a quantitative filter hybridization method. the hippurate hydrolysis test gave a poor correlation with the results of differentiation obtained by dna homology studies and outer membrane protein profile.19892745680
in vivo antigenic variation of campylobacter flagellin.campylobacter coli vc167 cells producing either antigenic phase 1 (p1) or phase 2 (p2) flagellins (as determined by characteristic protein and dna patterns) were used to infect rabbits by the removable intestinal tie-adult rabbit diarrhea (ritard) procedure. rabbits infected with p2 cells shed predominantly p2 cells throughout the infection; in rabbits infected with p1 cells, a transition of fecal isolates from p1 to p2 was observed.19892744863
immune response to campylobacter jejuni and campylobacter coli in a cohort of children from birth to 2 years of age.a cohort of 111 children from bangui, central african republic, was surveyed for enteric campylobacter infections from birth to the age of 2 years; stools were examined biweekly in these children until 6 months of age and at least four times per year thereafter until 2 years of age and after each diarrheal episode. blood samples were obtained at birth and at 3, 6, 9, 12, 18, and 24 months of age. antibodies against glycine-extracted membrane antigens, purified flagella, and cholera toxin (ct) we ...19892744860
diversity of serotypes of campylobacter jejuni and campylobacter coli isolated in laboratory animals.one hundred nineteen isolates of campylobacter jejuni and campylobacter coli from nine laboratory animal species were serotyped using antisera to 20 penner serotypes commonly isolated from cases of human enteric infections. although c. jejuni and c. coli were isolated from laboratory animals with diarrhea, the majority were cultured from asymptomatic animals (81%). seven of twenty-two isolates from animals with diarrhea were serotype 4 (32%) and three were serotype 1 (14%). sixty-one of the 119 ...19892724920
evidence for posttranslational modification and gene duplication of campylobacter flagellin.a gene encoding a flagellin protein of campylobacter coli vc167 has been cloned and sequenced. the gene was identified in a pbr322 library by hybridization to a synthetic oligonucleotide probe corresponding to amino acids 4 to 9 of the n-terminal sequence obtained by direct chemical analysis (s. m. logan, l. a. harris, and t. j. trust, j. bacteriol. 169:5072-5077, 1987). the dna was sequenced and shown to contain an open reading frame encoding a protein with a molecular weight of 58,945 and a le ...19892722741
serological analysis of the heat-stable antigens involved in serotyping campylobacter jejuni and campylobacter coli.analysis with serotyping antisera showed that carbohydrate determinants were the dominant heat-stable antigens of campylobacter jenuni/coli involved, whereas proteins did not contribute to the serological reactions. lipopolysaccharide (lps) along with a polysaccharide extract from whole bacteria (ps(wb] conferred strain serospecificity. in general, analysis with monoclonal antibodies in passive haemagglutination and co-agglutination tests showed the existence of similar antigenic determinants in ...19892713136
[antibiotic sensitivity of campylobacter jejuni and campylobacter coli strains with special reference to sensitivity to erythromycin and neomycin].166 strains of c. jejuni and 46 strains of c. coli were typed against 14 antibiotics using the agar diffusion test. all strains were resistant against penicillin g, oxacillin and rifampicin. susceptibility were found against chloramphenicol, erythromycin, neomycin, oxytetracyclin, streptomycin and gentamycin. the minimal inhibition concentration was performed for erythromycin and neomycin. all strains tested were inhibited by less than or equal to 2 micrograms/ml neomycin, one strain was resista ...19892710556
population genetics of human and animal enteric campylobacter strains.a total of 125 strains of campylobacter jejuni and campylobacter coli from human and animal hosts were analyzed by multilocus enzyme electrophoresis. ten genetic loci were characterized by the electrophoretic mobilities of their products, and all were found to be polymorphic. the nine loci considered for genetic analysis had an average allele number of 6.9 and a mean genetic diversity of 0.634. the strains were grouped into 64 electrophoretic types (ets) forming two principal clusters; the first ...19892707852
campylobacter infection in humans.campylobacter jejuni and campylobacter coli are now recognized as common bacterial cause of enteritis in humans. they infect the healthiest of persons resulting in a self-limiting diarrhoea often accompanied by mucus and blood in the stool. on the other hand c. fetus subsp fetus attacks otherwise debilitated persons whose body defences have been weakened by underlying conditions, and causes extragastrointestinal campylobacteriosis. c. pylori, which is able to survive in the stomach acid environm ...19892693070
a study of campylobacter jejuni and campylobacter coli in a river system.campylobacter isolates were detected in 82.1% of river water samples moderately polluted with organic wastes. in the majority of the samples less than 10 campylobacter cfu/100 ml were detectable. the presence of waterfowl and the faecal contamination from a poultry-farm resulted in considerable higher campylobacter contents (greater than 240/100 ml). correlation studies showed a high correlation coefficient (p less than 0.05) between the number of campylobacters and that of total coliforms. camp ...19892684204
a one-year study on thermophilic campylobacters isolated from fecal specimens.during a 12-month period, a systematic screening for thermophilic campylobacters was done on 5723 stool samples of hospitalized patients and out-patients who required stool culture tests for gastrointestinal disturbances. campylobacter strains were isolated in 130 samples (2.27%) and campylobacter jejuni (mainly biotype ii) was the species most frequently isolated. no pronounced seasonal pattern was evident and the age at which infection was more often observed was that from 6 to 24 months. eryt ...19892674635
[isolation of campylobacter jejuni and campylobacter coli from children with acute intestinal diseases].in this work the data obtained in the examination of 338 children with diarrhea, aged 5 days to 14 years, are presented. the methods used for the collection of samples and their storage till the moment of inoculation are described. the possibility of using the microscopic examination of campylobacter-containing native feces is shown. the work resulted in the isolation of 85 c. jejuni and c. coli strains. as shown in this work, the isolation of campylobacter depended on the age of children and th ...19892652954
characterization and antibiotic sensitivity of campylobacter jejuni and campylobacter coli isolated from children at the lagos university teaching hospital (luth), lagos, nigeria.one hundred and thirteen rectal swabs and 87 faecal samples obtained from the paediatric casualty of the lagos university teaching hospital, lagos, nigeria were processed for campylobacter jejuni/coli. twenty-two (11%) of the specimens were positive for c. jejuni. biotyping results showed that nine (41%) and eight (36%) belonged to c. jejuni biotypes i and ii respectively, while three (14%) and two (9%) belonged to c. coli biotypes i and ii. serotyping results revealed that c. jejuni biotypes i ...19892651685
infectoepidemilogic and immunologic implications of campylobacter coli enteritis in one newborn monozygotic twin pair.since campylobacter infections in humans at early age have not been thoroughly documented yet, it seemed interesting to report the occurrence of two simultaneous cases of c. coli enteritis in one newborn monozygotic twin pair. their clinical history, epidemiology and microbiological analysis, development of specific serum and mucosal immunity as well as total ig synthesis are discussed on the basis of their close genetic and environmental relationships.19892604587
characterization of the porins of campylobacter jejuni and campylobacter coli and implications for antibiotic susceptibility.the major outer membrane protein was extracted from campylobacter coli by triton x-100/edta fractionation of cell envelopes. this heat-modifiable protein was shown to have pore-forming activity in black lipid bilayers. the c. coli porin formed a relatively small cation-selective pore with a mean single-channel conductance of 0.53 +/- 0.16 ns in 1.0 m kcl. there was no evidence of oligomer formation, which suggested that each protein monomer formed a pore. pore-forming activity of the c. coli por ...19892543277
[in vitro susceptibility of campylobacter jejuni and campylobacter coli to quinolone antibiotics].in this study, in vitro susceptibilities of 24 campylobacter strains isolated from fecal samples were tested for quinolone antibiotics. 13 of the strains were identified as c. jejuni and 11 of them were c. coli. antibiotics which were included in this study were ciprofloxacin, ofloxacin, pefloxacin and fleroxacin. antibiotic susceptibility tests were performed on mueller-hinton agar (supplemented with 5% sheep blood) using the agar dilution method. results showed that quinolone antibiotics are v ...19892518646
coumamidines, new broad spectrum antibiotics of the cinodine type. iii. microbiologic activity of coumamidine gamma 1.the coumamidines are novel antibiotics with activity against a wide spectrum of aerobic gram-positive and gram-negative bacteria. all microbiological studies were performed on coumamidine gamma 1. the mic90s (micrograms/ml) of coumamidine are as follows: staphylococcus aureus 1.0, streptococcus pyogenes 8, enterobacteriaceae 2.0, pseudomonas aeruginosa 8, campylobacter jejuni and campylobacter coli 1, legionella pneumophila 8, haemophilus influenzae 0.5, neisseria gonorrhoeae 0.5. coumamidine ha ...19892498268
studies on the chemical constitution of cell wall lipo-oligosaccharide from campylobacter coli labet 227.the lipo-oligosaccharide (los) from campylobacter coli labet 227 was extracted by aqueous phenol. after delipidation and gel chromatography, two oligosaccharides were isolated. the higher molecular weight material os (i) which was estimated to contain six to seven sugar units was found to contain glucose, galactose, 2-acetamido-2-deoxyglucose, 2-acetamido-2-deoxygalactose and heptose. analysis of the partially methylated alditol acetates by g.c.-m.s. revealed the presence of terminal hexoses, a ...19892489074
oligonucleotide probe for detection and identification of campylobacter pylori.we have developed a novel and practical dna-rna hybridization assay for the detection and identification of campylobacter pylori in the gastric mucosa. this technique utilizes a [32p]ddatp-labeled synthetic oligonucleotide probe complementary to a nucleotide sequence present in c. pylori 16s rrna. this probe is very sensitive and reacted with all 23 strains of c. pylori tested. it is also highly specific, since there was no cross-reactivity with the heterologous organisms campylobacter coli, c. ...19892480360
comparison of methods used to separate the inner and outer membranes of cell envelopes of campylobacter spp.the outer membrane of campylobacter coli, c. jejuni and c. fetus cell envelopes appeared as three fractions after sucrose gradient centrifugation. each outer membrane fraction was contaminated with succinate dehydrogenase activity from the cytoplasmic membrane fraction. similarly the inner membrane fraction was contaminated with 2-ketodeoxyoctonate and outer membrane proteins including the porin(s). the separation of these two membranes was not facilitated by variations in lysozyme treatment, ce ...19882474628
isolation and characterization of campylobacter flagellins.sequential acid ph dissociation, differential ultracentrifugation, and neutral ph reassociation were used to partially purify serotypically distinct flagella from three strains of campylobacter jejuni and the two antigenic phases of flagella of campylobacter coli vc167. each c. jejuni flagellin and c. coli vc167 antigenic phase 1 flagellin were purified to homogeneity by reverse-phase high-performance liquid chromatography with a c8 spheri-10 column. c. coli vc167 antigenic phase 2 was purified ...19872444578
antigenic variation of campylobacter flagella.sodium dodecyl sulfate-polyacrylamide gel electrophoresis of flagella dissociated from strains of campylobacter coli and campylobacter jejuni belonging to the heat-labile serogroup lio 8 showed that some strains were capable of producing flagellin subunits of two different molecular weights (mrs), 59,500 and 61,500. immunoelectron microscopy of cultures of the type strain of this serogroup, c. coli vc167, showed the presence of two flagellum filaments of different antigenic specificity. epitopes ...19872444577
isolation and characterization of a common antigen in campylobacter jejuni and campylobacter coli.flagellin protein was isolated and purified from two serotype reference strains of campylobacter jejuni, pen 1 and pen 3. each preparation was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to consist of a diffuse band with a molecular mass of approximately 62 kilodaltons. antisera were prepared against flagellin from pen 1, and specific antibody was isolated by affinity chromatography with flagellin protein covalently bound to cyanogen bromide-activated sepharose. the high-a ...19862424932
interaction of campylobacter jejuni and campylobacter coli with lectins and blood group antibodies.lectins and blood group antibodies were used to probe the surface structures of campylobacter jejuni and campylobacter coli. of the 29 strains tested, there were distinct reaction patterns. the lectin-reactive and blood group antibody-reactive sites on the bacterial surface were distinguishable from the heat-stable (lipopolysaccharide) antigenic determinants. the interactions were strain specific. the reactive sites were stable with respect to culture media and passage and may be useful as addit ...19852410445
natural transformation in campylobacter species.growing cells of campylobacter coli and c. jejuni were naturally transformed by naked dna without the requirement for any special treatment. transformation frequencies for homologous chromosomal dna were approximately 10(-3) transformants per recipient cell in c. coli and 10(-4) in c. jejuni. maximum competence was found in the early log phase of growth. campylobacters preferentially took up their own dna in comparison with escherichia coli chromosomal dna, which was taken up very poorly. three ...19902404960
use of non-radioactive dna probes for detection of campylobacter jejuni and campylobacter coli in stool specimens.dna probes specific for c. jejuni (pdt1720 containing a 1475 base pair fragment) and for c. jejuni and c. coli (pdt1719 containing a 1845 base pair fragment) were isolated from a bacteriophage lambda gt11 genomic library of c. jejuni, using antiserum prepared against a 46 kda major outer membrane protein of c. jejuni. the two probe-fragments had different restriction maps and were only moderately related by dna hybridization analysis. a non-radioactive labelling kit which consisted of alkaline p ...19902402249
use of an alkaline phosphatase-labeled synthetic oligonucleotide probe for detection of campylobacter jejuni and campylobacter coli.a commercially available synthetic nucleic acid probe (snap) conjugated to alkaline phosphatase was compared with standard culture techniques for detecting campylobacter species. the snap was able to detect either 5 ng of c. jejuni dna or 10(5) cfu of bacteria. the snap could also detect dna extracted from 10(5) cfu in mock-infected stool samples. the snap detected c. jejuni and c. coli but showed no reactivity with c. laridis, c. fetus subsp. fetus, c. fetus subsp. venerealis, c. fennelliae, "c ...19902380381
purification, characterization, and localization of a protein antigen shared by thermophilic campylobacters.a protein antigen with an apparent molecular weight (mr) of 31,000 was isolated from 0.2 m glycine hydrochloride (ph 2.2) extracts of a typical human fecal isolate, campylobacter jejuni vc74. the protein was purified to homogeneity on a preparative scale by immunoaffinity chromatography followed by molecular sieving with a superose 12 column. isoelectric focusing under nondenaturing conditions indicated a pi of 9.3, and amino acid composition analysis showed that the protein was unusually rich i ...19902380360
campylobacter jejuni versus campylobacter coli in developing countries: how accurate are prevalence estimates? 19902373882
polynucleotide sequence relationships among flagellin genes of campylobacter jejuni and campylobacter coli.dna probes that encode a complete flagellin gene and various internal regions of the campylobacter coli vc167 flagellin genes were hybridized to 30 strains of c. coli or c. jejuni from 20 different lior serogroups. the results indicated a high overall degree of homology among all of the strains examined. although the most variable regions occurred within the middle of the gene, significant dna homology was observed among many serogroups in this region of the molecule.19902370114
evidence of reinfection with multiple strains of campylobacter jejuni and campylobacter coli in macaca nemestrina housed under hyperendemic conditions.a prospective bacteriologic study of 18 infant pig-tailed macaques (macaca nemestrina) housed in a nursery facility in which campylobacter spp. are endemic was undertaken to determine the epidemiology of infection and reinfection. the isolates of campylobacter jejuni and c. coli cultured from 8 of the 18 infants were characterized by serotyping, dna hybridization, and polyacrylamide gel electrophoresis protein profiles. the chronology of infection was indicative of multiple reinfections with dif ...19902365455
in vitro susceptibility of campylobacter jejuni and campylobacter coli isolated in austria to erythromycin and ciprofloxacin.more than 200 strains of campylobacter (c.) jejuni/coli isolated in 1985 and 1987/88 from human fecal specimens were tested for their susceptibility to erythromycin and ciprofloxacin. their mic90 as assessed by agar dilution tests was 2.0 and 0.5 mg/l, respectively. thus, all strains were regarded as susceptible to ciprofloxacin. with 2 out of 55 strains of c. coli the mic of erythromycin was 8.0 mg/l. therefore, only 3.6% of the c. coli strains were resistant to erythromycin. all 209 strains of ...19902360966
a cohort study of enteric campylobacter infection in children from birth to two years in bangui (central african republic).a cohort of 111 children from bangui, central african republic, was followed for enteric campylobacter infection from birth until the age of 2 years. stools were examined at each episode of diarrhoea, and bi-weekly up to the age of 6 months irrespective of the presence of diarrhoea. 349 episodes of diarrhoeal illness were recorded (1.6 per child-year). campylobacters were isolated from 41 (11.7%) of the 349 episodes, but in half of them another enteric pathogen was also isolated. campylobacters ...19902345912
genomic organization and expression of campylobacter flagellin genes.campylobacter coli vc167, which undergoes an antigenic flagellar variation, contains two full-length flagellin genes, flaa and flab, that are located adjacent to one another in a tandem orientation and are 91.5% homologous. the gene product of flab, which has an mr of 58,946, has 93% sequence homology to the gene product of flaa, which has an mr of 58,916 (s. m. logan, t. j. trust, and p. guerry, j. bacteriol. 171:3031-3038, 1989). mutational analyses and primer extension experiments indicated t ...19902318805
isolation and antimicrobial susceptibility of campylobacter coli and campylobacter jejuni from slaughter hogs.cultural examination of cecal contents from 109 market weight hogs slaughtered in prince edward island during may-july 1988 yielded 62 isolates of campylobacter coli and seven campylobacter jejuni. a commercial latex agglutination test helped to confirm the identification of campylobacter. when tested against four drugs: erythromycin, tetracycline, kanamycin and ampicillin, 11 isolates showed multiple resistance. resistance to erythromycin was seen in 19% and 28.6% of campylobacter coli and camp ...19902308529
adherence, enterotoxigenicity, invasiveness and serogroups in campylobacter jejuni and campylobacter coli strains from adult humans with acute enterocolitis.two hundred campylobacter jejuni and campylobacter coli strains from the same number of adult swedish patients with acute enterocolitis were tested regarding adherence to and invasiveness in hep-2 cells and for enterotoxigenicity by the cho-cell assay. the serogroup characteristics, heat-stable and heat-labile, for each strain were also investigated. eighty-four percent of the strains were classified as c. jejuni and 16 percent as c. coli. all of the strains were adherent to hep-2 cells, 39% wer ...19902302354
tropical arthritis in papua new guinea: a reactive arthritis.an idiopathic, asymmetrical oligoarthritis affecting young adults is prevalent in the tropical regions of africa, asia and melanesia. a serological study was undertaken in 23 consecutive patients with polyarthritis. each patient was assigned two paired control subjects. a standardized history, physical examination and investigations were performed. acute and convalescent sera were examined for evidence of recent infection. the presence of hla-b27 was determined. twelve (52%) of the 23 patients h ...19902300025
[evaluation of a new medium using red-cell lysate for the isolation of campylobacter jejuni and campylobacter coli].a semiprepared medium containing horse globular lysate was evaluated for the isolation of campylobacter under two incubation atmospheres (5% o2 and candle jar). studying 19 known strains of c. jejuni, 151 human and 230 animal fecal samples. they were seeded on conventional medium (mc) and on the new medium (mlg) and incubated at 42 degrees c for 24 and 48 h. campylobacter strains grew better in 5% o2 atmosphere after 48 h of incubation. all the strains that grew or were isolated on mc, could als ...19902287716
use of tri-gas incubator for routine culture of campylobacter species from fecal specimens.we evaluated a tri-gas incubator for campylobacter isolation to be used instead of an anaerobic jar. fecal specimens were cultured in duplicate onto charcoal selective medium and incubated at 43 degrees c for 48 h in two different environments: a tri-gas incubator (forma scientific) adjusted to provide an atmosphere of 10% co2, 10% o2, and the balance n2; and evacuated anaerobic jars with a replacement gas mixture of 10% co2, 5% o2, and 85% n2. a total of 106 campylobacter jejuni and 8 campyloba ...19902280012
chloramphenicol resistance in campylobacter coli: nucleotide sequence, expression, and cloning vector construction.a chloramphenicol-resistance determinant (cmr), originally cloned from campylobacter coli plasmid pnr9589 in japan, was isolated and the nucleotide sequence determined, which contained an open reading frame of 621 bp. the gene product was identified as cm acetyltransferase (cat), which had a putative amino acid sequence that showed 43% to 57% identity with other cat proteins of both gram+ and gram- origin. although expression of the cat gene was constitutive in both c. coli and escherichia coli, ...19902227449
evaluation of cytotoxic activity in fecal filtrates from patients with campylobacter jejuni or campylobacter coli enteritis.we sought to determine the prevalence of cytotoxic activity in fecal filtrates from persons with c. jejuni or c. coli enteritis. stool specimens were collected from 20 persons with c. jejuni or c. coli enteritis, 20 persons with acute diarrheal illnesses of other causes, and 9 healthy, asymptomatic persons. fecal filtrates were then incubated with chinese hamster ovary (cho) or hela cells. the fecal filtrate from 1 of the 20 (5%) persons with campylobacter enteritis was cytotoxic for hela cells ...19902227365
comparative in vitro activity of cefpirome and cefepime, two new cephalosporins.in in vitro tests the broad-spectrum cephalosporins cefpirome and cefepime were highly active against enterobacteriaceae, although often less so against strains resistant to amoxicillin-clavulanate and ticarcillin-clavulanate, and against most strains of acinetobacter spp. and aeromonas hydrophila. they were also active against pseudomonas aeruginosa, although strains with non-plasmid mediated beta-lactam resistance were sometimes less sensitive. other pseudomonas spp. varied in their sensitivit ...19902226498
morphology and immunoperoxidase studies of intestinal adenomatosis in the blue fox, alopex lagopus.in a blue fox farm about 300 of 400 weanling pups were affected with diarrhoea and prolapse of the rectum. about 40 pups died in an emaciated state after a few days of disease. eight pups were submitted for examination. pathological findings were restricted to the caecum, proximal colon and rectum, with occasional extension to the ileum. affected areas revealed a thick and rigid intestinal wall, a narrow lumen and a thickened, wrinkled and sometimes ulcerated mucosa. histologically, the mucosa s ...19902195067
biotypes, serogroups and antibiotic susceptibility of campylobacter jejuni and campylobacter coli in chile.phenotypic markers were studied in 105 strains of thermophilic campylobacters isolated from human beings, animals and drinking water in santiago, chile. the strains were identified as campylobacter jejuni (n = 49) and campylobacter coli (n = 56). biotypes i and ii (lior schema) accounted for 96% c. jejuni isolates, the other 4% being biotype iv but the two biotypes of c. coli were about equally represented. a total of 28 serogroups (lior's heat-labile antigens) were identified. lior 13, 9, 79, 2 ...19902181024
emergence of aminoglycoside resistance genes aada and aade in the genus campylobacter.resistance to streptomycin or spectinomycin or both in five campylobacter coli strains, two campylobacter jejuni strains, and a campylobacter-like strain was studied by enzymatic assays and dot blot hybridization. resistance was due to 6- or 3",9-aminoglycoside adenylyltransferases and to new types of phospho- and adenylyltransferases.19902168151
electrolyte abnormalities associated with diarrhea in rhesus monkeys: 100 cases (1986-1987).serum electrolyte values from 100 rhesus monkeys with diarrhea were reviewed. the most frequent finding was hyponatremia (88%), with hypochloremia next most frequently detected (80%). metabolic acidosis was less common (59%) and usually associated with high anion gap values. associations between electrolyte abnormalities and age, housing, or case outcome were not found. bacteriologic culturing was performed on fecal specimens from 90 monkeys. campylobacter coli or c jejuni alone was isolated fro ...19902112122
immuno-histochemical and -cytochemical evidence suggesting the presence of campylobacter jejuni and campylobacter coli in cases of porcine intestinal adenomatosis.antisera against a number of campylobacter species were used in immuno-histochemical and -cytochemical studies on cases of porcine intestinal adenomatosis. avidin-biotin-complex (abc) and streptavidin immunoperoxidase methods were used on formalin-fixed, paraffin-embedded and frozen sections. protein a gold method was used on formaldehyde fixed and frozen sections for immuno-cytochemistry. the antisera used were raised in rabbits by subcutaneous or intravenous injection of living or formalin tre ...19902099622
isolation of campylobacter coli from a proliferative intestinal lesion in a ferret. 19902094455
[evaluation of a latex agglutination method for the rapid identification of campylobacter jejuni/coli].a latex agglutination assay was developed to identify campylobacter jejuni and campylobacter coli. we evaluated the specificity, reproducibility and utility of the assay for clinical use and the following results were obtained. 1) to prepare standardized antigen, bacterial cells must be suspended to a density of 1 to 5 mcfarland unit, and heated at 121 degrees c for 10 to 30 min. 2) bacterial cells may be suspended either in the solution provided with the kit, or in physiological saline, without ...19902082036
identification of campylobacter jejuni and campylobacter coli from waste water by sds-disc-page of whole cell protein.analysis of the electrophoretic profiles of whole cell proteins of campylobacter strains from waste water was used to differentiate c. jejuni from c. coli. the results show, that biotyping of campylobacters from waste water may results in a false species differentiation. some of the campylobacter strains originally typed as c. coli, were proved to be atypical c. jejuni strains. therefore, in addition to biotyping and serotyping, electrophoretic typing is recommended to ensure correct identificat ...19902080967
significance of flagella in colonization resistance of rabbits immunized with campylobacter spp.cross-protection among different lior and penner serogroups of campylobacter spp. was studied. rabbits were orally immunized by gastric feeding with campylobacter spp., and 27 to 30 days later, they were challenged with matched or unmatched serogroups by the removable intestinal tie adult rabbit diarrhea (ritard) procedure. when immunized animals were challenged with different lior serotypes, no protection against colonization was seen; however, when challenged with homologous lior serogroups, p ...19912050397
relationship between the clostridium perfringens catq gene product and chloramphenicol acetyltransferases from other bacteria.the nucleotide sequence of the clostridium perfringens chloramphenicol acetyltransferase (cat)-encoding resistance determinant, catq, was determined. an open reading frame encoding a protein of 219 amino acids with a molecular weight of 26,014 was identified. although catq was expressed constitutively, sequences similar in structure to those found upstream of inducible cat genes were observed. the catq gene was distinct from the c. perfringens catp determinant. the deduced catq monomer had consi ...19912039197
pulsed-field gel electrophoresis of campylobacter jejuni and campylobacter coli genomic dna and its epidemiologic application.genomic dna from 12 different campylobacter jejuni and 10 campylobacter coli isolates was digested with smai and analyzed by pulsed-field gel electrophoresis (pfge) and 16s rrna hybridization studies. although the two campylobacter species displayed species-specific restriction and hybridization patterns, significant intraspecies differences were observed. combined pfge and hybridization pattern analysis failed to provide any more epidemiologic information than was obtained from pfge restriction ...19912019755
isolation of group 2 aerotolerant campylobacter species from thai children with diarrhea.campylobacter species were isolated from 93 (15%) of 631 thai children with diarrhea using the membrane filter technique on nonselective blood agar incubated at 37 degrees c. campylobacter jejuni was isolated from 62 (10%), campylobacter coli from 14 (2%), and atypical campylobacters from 17 (3%). the 17 atypical strains were first characterized biochemically and by dot blot dna hybridization. catalase-negative strains also were characterized by dna hybridization and ribotype pattern. one strain ...19912019754
common and variable domains of the flagellin gene, flaa, in campylobacter jejuni.the organization of the flagellin gene locus in campylobacter jejuni strain in1 (lior 7) was determined using the polymerase chain (pcr) reaction and a series of oligonucleotide primers. two tandemly arranged flagellin genes of approximately 1.7 kb were found to be joined by an intervening segment of c.0.2kb, similar to that reported for campylobacter coli. the 5' flagellin gene, flaa, was generated by pcr and both strands sequenced. comparison of the deduced amino acid sequence for c. jejuni fl ...19911956293
immunocytochemical identification of helicobacter pylori in formalin-fixed gastric biopsies.h&e and special histochemical stains are used by most laboratories to identify helicobacter pylori (h. pylori) in gastric biopsy specimens. however, background staining can complicate recognition of h. pylori and small numbers of organisms may be overlooked. additionally, histochemical stains do not distinguish h. pylori from other spiral organisms. we investigated two commercially available monoclonal antibodies, one directed against campylobacter coli and c. jejuni (mab002) and the other again ...19911924280
comparison of the survival of campylobacter jejuni and campylobacter coli in culturable form in surface water.six campylobacter jejuni and six campylobacter coli strains were isolated from cows and pigs, and their survival in lake water was compared by viable counts. campylobacter jejuni survived longer in culturable form than c. coli in untreated and membrane-filtered water both at 4 and 20 degrees c. this difference in survival time may be a reason why c. jejuni is generally isolated from surface waters more frequently than c. coli. both species survived better in filtered than in untreated water. thi ...19911913357
identification, purification, and characterization of major antigenic proteins of campylobacter jejuni.evidence from developing countries and volunteer studies indicates that immunity to campylobacter jejuni and campylobacter coli may be acquired, but the antigenic basis for this protection is poorly defined. we have purified to homogeneity four proteins with molecular weights of 28,000 (peb1), 29,000 (peb2), 30,000 (peb3), and 31,000 (peb4) from epidemic c. jejuni strain 81-176 using acid extraction and sequential ion-exchange, hydrophobic interaction, and gel filtration chromatography. the rela ...19911885571
use of embryonating eggs for isolation of campylobacter species from intestines of swine with proliferative enteritis.intestinal tissues from swine affected with proliferative enteritis were ground, filtered through a 0.65-micron pore membrane filter, diluted, and injected into 7-day-old embryonated hens' eggs via the yolk sac. at 2, 4, and 7 days later, yolk sac swab specimens taken from live embryos were cultured for campylobacter species. campylobacter hyointestinalis was recovered from eggs injected with tissues of swine with acute hemorrhagic proliferative enteritis at dilutions up to 10(-4). campylobacter ...19911883083
incidence of toxigenic campylobacter strains in south africa.campylobacter strains can produce a heat-labile cytotonic toxin (cton) and various cytotoxins (ctox). of 22 south african campylobacter strains tested, 86% were toxigenic (77% produced cton, 41% produced ctox, and 32% produced both types) and 14% were toxin negative. campylobacter jejuni strains were 67% cton positive and 47% ctox positive, whereas campylobacter coli strains were 100 and 29% positive, respectively.19911864947
role of two flagellin genes in campylobacter motility.campylobacter coli vc167 t2 has two flagellin genes, flaa and flab, which share 91.9% sequence identity. the flaa gene is transcribed from a o-28 promoter, and the flab gene from a o-54 promoter. gene replacement mutagenesis techniques were used to generate flaa+ flab and flaa flab+ mutants. both gene products are capable of assembling independently into functional filaments. a flagellar filament composed exclusively of the flaa gene product is indistinguishable in length from that of the wild t ...19911856171
identification of campylobacter jejuni and campylobacter coli antigens with mucosal and systemic antibodies.the development of a rapid and specific diagnostic assay for campylobacter infections is important in determining the etiology of acute diarrhea in humans. studies have shown that sonicated whole bacteria or partially purified antigens cross-reacted with antibodies against other closely related bacteria. to solve the problems of specificity, we identified specific antigens of campylobacter jejuni and campylobacter coli for use in diagnostic assays. we investigated the responses of serum, urine, ...19911855976
a noti restriction site of campylobacter coli chromosomal dna detected by double-digestion analysis with apai. 19911832048
[morphological changes of campylobacter jejuni and campylobacter coli under various culture conditions and the accompanied changes in the cell composition].the morphological transformation from the spiral form to the coccoidal form campylobacter jejuni and campylobacter coli was studied under various conditions by such techniques as electron microscopy, sds-polyacrylamide gel electrophoresis (sds-page), and chemical analyses. the conversion from the spiral form to the coccoidal form of campylobacter in microaerophilic cultivation occurred in about 50% of the cells in 48 h and in about 90% of the cells in 72 h. at higher temperatures (37 c and 42 c) ...19911805043
development of resistance to quinolones in five patients with campylobacteriosis treated with norfloxacin or ciprofloxacin.development of resistance to nalidixic acid, norfloxacin and ciprofloxacin was observed in five patients with campylobacter jejuni or campylobacter coli infection. from all these patients nalidixic acid- and quinolone-susceptible strains were isolated initially, whereas after therapy with norfloxacin or ciprofloxacin strains resistant to these antibiotics were found. campylobacter strains from the same patient always belonged to the same species and, with the exception of one case, showed identi ...19911794366
analysis of the role of flagella in the heat-labile lior serotyping scheme of thermophilic campylobacters by mutant allele exchange.flagellin mutations originally constructed in campylobacter coli vc167 (serotype lio8) by a gene replacement mutagenesis technique (p. guerry, s. m. logan, s. thornton, and t. j. trust, j. bacteriol. 172:1853-1860, 1990) were moved from the original host into campylobacter strains of a number of other lior serogroups by a natural transformation procedure. this is the first report of the use of this transformation method to transfer a mutated locus among campylobacter strains. flagellin mutants w ...19911774247
emergence of fluoroquinolone resistance in campylobacter jejuni and campylobacter coli in subjects from finland.the in vitro susceptibilities of 102 human campylobacter strains isolated between 1978 and 1980 and 100 strains isolated in 1990 to ciprofloxacin, norfloxacin, erythromycin, gentamicin, and doxycycline were examined. the biotypes and heat-stable serotypes of the strains as well as antimicrobial treatments and travel history of the campylobacter-positive patients were also studied. the results indicated that susceptibility to erythromycin, gentamicin, and doxycycline has remained the same during ...19911759828
characterization of erythromycin resistance in campylobacter jejuni and campylobacter coli.the mechanism of resistance to erythromycin, the drug of choice in the treatment of campylobacter gastroenteritis, was investigated. erythromycin resistance (mics, greater than 1,024 micrograms/ml) in three clinical isolates of campylobacter jejuni and one c. coli isolate was determined to be constitutive and chromosomally mediated. in vivo protein synthesis in erythromycin-susceptible c. jejuni and c. coli strains was completely inhibited by low levels of erythromycin (5 micrograms/ml), whereas ...19911759819
potential sources of campylobacter species in the homes of farmworkers in zimbabwe.chicken faeces, houseflies, household stored drinking water, cooked or prepared foods and handwashings of people of all age groups and hands of children under 5 years old were examined as potential sources and transmission routes of campylobacter species in a farmworker community. campylobacter species were found to be common in chicken faeces collected from the homesteads of the farmworkers but were rarely isolated from houseflies or household stored drinking water. they were not isolated from ...19911758009
fluoroquinolone resistance in campylobacter jejuni and campylobacter coli isolated from human faeces in switzerland. 19911753149
a note on the comparative efficacy of three selective media for isolation of campylobacter species from environmental samples.the efficacy of three selective broths and agars, i.e. preston-, mccd- and car medium, were compared to each other in various combinations. twelve campylobacter coli and 9 campylobacter jejuni strains that had been isolated from sewage were used as test strains. to evaluate the applicability for highly contaminated environmental samples 15 sewage samples were examined, subsequently. significant differences between the media could not be ascertained. the application of preston broth/agar or car b ...19911750964
a dna sequence upstream of the tet(o) gene is required for full expression of tetracycline resistance.the dna sequences upstream of the tet(o) and tet(m) open reading frames (orfs) (ca. 300 bp) were found to share a higher degree of homology than those of the tet(o) and tet(m) orfs themselves. a transcription initiation site for tet(o) was located by primer extension analysis. campylobacter coli was found to use a promoter sequence different from that used by escherichia coli. the sequence upstream of tet(o) was shown to be required in cis for high-level resistance to tetracycline.19911722078
evaluation of a simplified procedure for serotyping campylobacter jejuni and campylobacter coli which is based on the o antigen.a simplified procedure for serotyping campylobacter jejuni and campylobacter coli on the basis of thermostable antigens was developed and tested for its applicability as a routine typing method. the assay involves the sensitization of erythrocytes with an antigenic extract and performance of a slide agglutination assay with specific antisera. in order to simplify the typing system to a greater extent, the standard typing antisera were pooled into nine groups for c. jejuni and four groups for c. ...19911719020
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