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isolation of campylobacter spp. from client-owned dogs and cats, and retail raw meat pet food in the manawatu, new zealand.campylobacter causes acute gastroenteritis in people worldwide and is frequently isolated from food, animals and the environment. the disease is predominately food-borne but many routes of transmission and sources of infection have been described, including contact with pets. the prevalence of campylobacter spp. in dogs and cats varies widely, and data on new zealand pets are limited. this study aimed to investigate the prevalence of campylobacter spp. in dogs, cats and retail raw meat pet food ...201627860343
[detection of biofilm formation by selected pathogens relevant to the food industry].detection of biofilm formation by microbial pathogens relevant to the food industry and comparison of biofilm formation under different conditions of culture.201526448305
occurrence and characteristics of fastidious campylobacteraceae species in porcine samples.this study investigated the prevalence and characteristics of campylobacteraceae including a range of fastidious species in porcine samples. over a thirteen month period caecal contents (n=402) and pork carcass swabs (n=401) were collected from three pork abattoirs and pork products (n=399) were purchased at point of sale in the republic of ireland. campylobacteraceae isolates were recovered by enrichment, membrane filtration and incubation in antibiotic free media under a modified atmosphere (3 ...201323474652
assessment of the prevalence and diversity of emergent campylobacteria in human stool samples using a combination of traditional and molecular methods.this study aims to assess the diversity of campylobacteria (campylobacter and arcobacter) in human fecal samples from patients with diarrhea (n = 140) and asymptomatic controls (n = 116) in chile, using a combination of traditional culture and molecular methods. the culture methods detected campylobacteria in 10.7% of the patients with diarrhea and in 1.7% of the controls. in contrast, the molecular methods detected campylobacteria more often than the traditional culture, with a prevalence of 25 ...201323375869
simultaneous identification of 13 foodborne pathogens by using capillary electrophoresis-single strand conformation polymorphism coupled with multiplex ligation-dependent probe amplification and its application in foods.capillary electrophoresis-single strand conformation polymorphism (ce-sscp) coupled with stuffer-free multiplex ligation-dependent probe amplification (mlpa) was developed to identify 13 species of foodborne pathogens simultaneously. species-specific mlpa probes were designed for nine of these species. these probes were targeted to the groel, glya, mms, tuf, inv, ipah, nuc, vvh, and 16s rrna genes, which corresponded to bacillus cereus, campylobacter coli, cronobacter sakazakii, enterococcus spp ...201627494415
a rapid and simple real-time pcr assay for detecting foodborne pathogenic bacteria in human feces.a rapid, simple method for detecting foodborne pathogenic bacteria in human feces is greatly needed. here, we examined the efficacy of a method that employs a combination of a commercial pcr master mix, which is insensitive to pcr inhibitors, and a dna extraction method which used sodium dodecyl benzene sulfonate (sdbs), and tween 20 to counteract the inhibitory effects of sdbs on the pcr assay. this method could detect the target genes (stx1 and stx2 of enterohemorrhagic escherichia coli, inva ...201626902211
phenolic acids profile, antioxidant and antibacterial activity of chamomile, common yarrow and immortelle (asteraceae).chamomile, common yarrow and immortelle ethanolic extracts were chemically analysed with respect to phenolics. twelve phenolic acids were separated and identified by hplc-dad and the presence of rosmarinic acid was additionally confirmed by lc-ms. five methods were applied for the evaluation of extracts' antioxidant properties (frap, dpph, abts, chelating activity, briggs-rauscher reaction), while the antibacterial activity was tested against some of the major food-borne pathogens (campylobacter ...201425632475
development of 23 individual taqman® real-time pcr assays for identifying common foodborne pathogens using a single set of amplification conditions.most of the acute intestinal diseases are caused by foodborne pathogens with infants and elderly people being at major risk. the aim of this study was to develop a procedure to simultaneously detect 20 foodborne pathogens in complex alimentary matrices such as milk, cheese and meat. the list of targets include, among the others, listeria spp., salmonella spp., shigella spp., escherichia coli spp., campylobacter spp., clostridium spp. and staphylococcus aureus. the accuracy of detection was deter ...201424929880
[identification of zoonotic bacterial pathogens by the maldi tof ms method].to verify whether the maldi tof ms method can be used for rapid identification of selected zoonotic bacterial pathogens isolated from various types of materials in the real conditions of routine laboratory work.201526312374
further characterization and independent validation of a dna aptamer-quantum dot-based magnetic sandwich assay for campylobacter.previously reported dna aptamers developed against surface proteins extracted from campylobacter jejuni were further characterized by aptamer-based western blotting and shown to bind epitopes on proteins weighing ~16 and 60 kd from reduced c. jejuni and campylobacter coli lysates. proteins of these approximate weights have also been identified in traditional antibody-based western blots of campylobacter spp. specificity of the capture and reporter aptamers from the previous report was further va ...201728342148
prevalence, antimicrobial resistance and genetic diversity of campylobacter coli and campylobacter jejuni in ecuadorian broilers at slaughter age.thermotolerant campylobacter spp. are a major cause of foodborne gastrointestinal infections worldwide. the linkage of human campylobacteriosis and poultry has been widely described. in this study we aimed to investigate the prevalence, antimicrobial resistance and genetic diversity of c. coli and c. jejuni in broilers from ecuador. caecal content from 379 randomly selected broiler batches originating from 115 farms were collected from 6 slaughterhouses located in the province of pichincha durin ...201728339716
a quantitative real-time pcr approach for assessing campylobacter jejuni and campylobacter coli colonization in broiler herds.human campylobacteriosis is a major public health concern in developed countries, with campylobacter jejuni and campylobacter coli from poultry recognized as the main source of human infection. identification of campylobacter-positive broiler herds before slaughter is essential for implementing measures to avoid carryover of pathogens via the slaughter process into the food chain. however, appropriate methods that have been validated for testing poultry flocks antemortem are lacking for campylob ...201728282226
variation in campylobacter multilocus sequence typing subtypes from chickens as detected on three plating media.the objective of this study was to compare subtypes of campylobacter jejuni and campylobacter coli detected on three selective campylobacter plating media to determine whether each medium selected for different subtypes. fifty ceca and 50 carcasses (representing 50 flocks) were collected from the evisceration line in a commercial broiler processing plant. campylobacter was cultured and isolated from cecal contents and carcass rinses on campy-cefex, campy line, and rf campylobacter jejuni/coli ag ...201628221919
whole genome sequencing and analysis of campylobacter coli yh502 from retail chicken reveals a plasmid-borne type vi secretion system.campylobacter is a major cause of foodborne illnesses worldwide. campylobacter infections, commonly caused by ingestion of undercooked poultry and meat products, can lead to gastroenteritis and chronic reactive arthritis in humans. whole genome sequencing (wgs) is a powerful technology that provides comprehensive genetic information about bacteria and is increasingly being applied to study foodborne pathogens: e.g., evolution, epidemiology/outbreak investigation, and detection. herein we report ...201728217442
temporal induction of pro-inflammatory and regulatory cytokines in human peripheral blood mononuclear cells by campylobacter jejuni and campylobacter coli.campylobacter jejuni along with c. coli are major cause of human gastroenteritis worldwide. so far, the human immune response against campylobacter is not entirely clear. we hypothesize that it is coordinated by an interaction between pro-inflammatory and regulatory cytokines which is influenced by bacterial and host-individual differences. accordingly, we used peripheral blood mononuclear cells (pbmc) from healthy donors to study the primary systemic immune response to c. jejuni and c. coli. pb ...201728196097
genetic diversity and antimicrobial resistance profiles of campylobacter coli and campylobacter jejuni isolated from broiler chicken in farms and at time of slaughter in central italy.genetic diversity and antimicrobial resistance of campylobacter coli and campylobacter jejuni were investigated along the broiler chicken production chain in central italy.201728194914
isolation, identification and differentiation of campylobacter spp. using multiplex pcr assay from goats in khartoum state, sudan.the aim of this study was to identify and characterize thermophilic campylobacter species in faecal samples from goats in khartoum state, sudan, by application of multiplex polymerase chain reaction. campylobacteriosis is a zoonotic disease of global concern, and the organisms can be transmitted to human via food, water and through contact with farm animals and pets. there are five clinically related campylobacter species: campylobacter jejuni (c. jejuni). campylobacter coli, campylobacter lari, ...201728160162
reply to "activity of fosfomycin on clinical isolates of campylobacter jejuni and campylobacter coli of enteric origin". 201728119318
activity of fosfomycin on clinical isolates of campylobacter jejuni and campylobacter coli of enteric origin. 201728119317
prevalence of thermotolerant campylobacter spp. in chicken meat in croatia and multilocus sequence typing of a small subset of campylobacter jejuni and campylobacter coli isolates.in order to detect thermotolerant campylobacter spp., 241 samples of fresh chicken meat, at retail in croatia, were analysed according to a standard method, followed by biochemical test and molecular polymerase chain reaction/restriction enzyme analysis for exact species determination. campylobacter spp. prevalence was 73.86%. campylobacter jejuni and campylobacter coli were isolated from 53.53 and 15.35% of the samples, respectively. in 4.98% of isolates thermotolerant campylobacter spp. were n ...201628115906
restriction glycosylases: involvement of endonuclease activities in the restriction process.all restriction enzymes examined are phosphodiesterases generating 3'-oh and 5'-p ends, but one restriction enzyme (restriction glycosylase) excises unmethylated bases from its recognition sequence. whether its restriction activity involves endonucleolytic cleavage remains unclear. one report on this enzyme, r.pabi from a hyperthermophile, ascribed the breakage to high temperature while another showed its weak ap lyase activity generates atypical ends. here, we addressed this issue in mesophiles ...201627980098
identification of a campylobacter coli methyltransferase targeting adenines at gatc sites.campylobacter coli can infect humans and colonize multiple other animals but its host-associated genes or adaptations are poorly understood. adenine methylation at gatc sites, resulting in mboi resistance of genomic dna, was earlier frequently detected among c. coli from swine but not among turkey-derived isolates. the underlying genetic basis has remained unknown. comparative genome sequence analyses of c. coli 6461, a swine-derived strain with mboi-resistant dna, revealed two chromosomal orfs, ...201627915251
genomic variations leading to alterations in cell morphology of campylobacter spp.campylobacter jejuni, the most common cause of bacterial diarrhoeal disease, is normally helical. however, it can also adopt straight rod, elongated helical and coccoid forms. studying how helical morphology is generated, and how it switches between its different forms, is an important objective for understanding this pathogen. here, we aimed to determine the genetic factors involved in generating the helical shape of campylobacter. a c. jejuni transposon (tn) mutant library was screened for non ...201627910897
antimicrobial resistance of campylobacter species isolated from broilers in live bird markets in shanghai, china.this study was conducted to determine the prevalence of antimicrobial resistance in campylobacter spp. isolates from broilers in live bird markets (lbms). a total of 209 campylobacter spp. isolates (84 campylobacter jejuni; 125 campylobacter coli) were recovered from 364 broiler cecum samples collected from five lbms in shanghai, china. minimum inhibitory concentrations of 13 antimicrobials were determined using agar dilution method. more than 96% of the campylobacter spp. isolates were resistan ...201727854542
sensitivity of direct culture, enrichment and pcr for detection of campylobacter jejuni and c. coli in broiler flocks at slaughter.broiler chicken flocks are a significant source of campylobacter jejuni and campylobacter coli that result in the major public health problem of campylobacteriosis. accurate estimates of the prevalence of both c. coli and c. jejuni in flocks would enhance epidemiological understanding, risk assessment and control options. this study combined results from a panel of 10 detection tests (direct culture, enrichment and pcr) on caecal samples from flocks at slaughter. a parallel interpretation approa ...201627770505
occurrence and genotypes of campylobacter species in broilers during the rearing period.poultry are the main source of campylobacter infection worldwide. to obtain information on campylobacter-infected flocks and create a reference for preventing and controlling campylobacter at farm level, campylobacter isolates were recovered from broilers and the environments of nine chicken flocks in two farms during growth. the genetic relationship between the campylobacter isolates was determined using multilocus sequence typing. flocks were colonized as early as 3 weeks after introduction to ...201727766896
[multilocus sequence typing analysis of human campylobacter coli in granada (spain)].different subtypes of campylobacter spp. have been associated with diarrhoea and a multilocus sequence typing (mlst) method has been performed for subtyping. in the present work, mlst was used to analyse the genetic diversity of eight strains of campylobacter coli.201627701619
sensitivity to enterocins of thermophilic campylobacter spp. from different poultry species.campylobacter spp. have been isolated from various animals, including poultry. they are rapidly transmitted throughout broiler sheds by the fecal-oral route. a promising strategy to reduce campylobacter spp. in poultry may be done due to the beneficial properties of probiotic bacteria and their bacteriocins. in this study, inhibition spectrum/activity of different enterocins was evaluated against campylobacter spp. (isolated from different poultry) to indicate further practical use of enterocins ...201627602434
macrolide resistance mechanisms and virulence factors in erythromycin-resistant campylobacter species isolated from chicken and swine feces and carcasses.resistance to antimicrobials was measured in 73 isolates of campylobacter jejuni (c. jejuni) and 121 isolates of campylobacter coli (c. coli) from chicken and swine feces and carcasses in korea. both bacterial species showed the highest resistance to (fluoro) quinolones (ciprofloxacin and nalidixic acid) out of the nine antimicrobials tested. erythromycin resistance was much higher in c. coli (19.0%, 23/121) than in c. jejuni (6.8%, 5/73). the mutation in the 23s rrna gene was primarily responsi ...201727593510
prevalence, phenotypic and genetic diversity of campylobacter in poultry fresh meat and poultry products on retail sale in tuscany (italy).in this study, the prevalence of campylobacter spp. in poultry fresh meat and ready‑to‑cook products was evaluated. seventy‑three samples were collected at retail level from supermarkets and discount stores, obtaining 61.6% positivity. of 133 campylobacter isolates, 86 strains (campylobacter coli, 58.1% and campylobacter jejuni, 41.9%) were selected for characterisation on the basis of their smai and kpni pulsed field gel‑electrophoresis (pfge) profiles, to exclude clonal replicates. campylobact ...201728365923
faecal campylobacter shedding among dogs in animal shelters across texas.epidemiologic studies on faecal campylobacter shedding among dogs in the united states have been limited, despite evidence that the incidence of human campylobacteriosis has increased over the last decade. our objectives were to estimate the prevalence of faecal campylobacter shedding among shelter dogs in texas, to estimate the specific prevalence of campylobacter jejuni and campylobacter coli shedding, and to identify risk factors for campylobacter-positive status. using a cross-sectional stud ...201728371319
influence of enrichment and isolation media on the detection of campylobacter spp. in naturally contaminated chicken samples.investigating campylobacter epidemiology requires adequate technique and media to ensure optimal culturing and accurate detection and isolation of campylobacter strains. in the present study, we investigated the performances of three enrichment durations in bolton broth (0, 24 and 48h) and compared four isolation media (mccda, karmali, butzler no. 2 and campyfood agar (cfa)) for the detection of campylobacter positive samples and the identification of campylobacter species, from naturally contam ...201627373751
clinical relevance of infections with zoonotic and human oral species of campylobacter.genus campylobacter has been recognized as a causative bacterial agent of animal and human diseases. human campylobacter infections have caused more concern. campylobacters can be classified into two groups in terms of their original host: zoonotic and human oral species. the major zoonotic species are campylobacter jejuni and campylobacter coli, which mostly reside in the intestines of avian species and are transmitted to humans via consumption of contaminated poultry products, thus causing hum ...201627350611
monitoring antimicrobial resistance in the food supply chain and its implications for fda policy initiatives.in response to concerning increases in antimicrobial resistance (amr), the food and drug administration (fda) has decided to increase veterinary oversight requirements for antimicrobials and restrict their use in growth promotion. given the high stakes of this policy for the food supply, economy, and human and veterinary health, it is important to rigorously assess the effects of this policy. we have undertaken a detailed analysis of data provided by the national antimicrobial resistance monitor ...201627324772
evaluation of a multiplex real-time pcr assay for detecting major bacterial enteric pathogens in fecal specimens: intestinal inflammation and bacterial load are correlated in campylobacter infections.a total of 1,056 native or cary-blair-preserved stool specimens were simultaneously tested by conventional stool culturing and by enteric bacterial panel (ebp) multiplex real-time pcr for campylobacter jejuni, campylobacter coli, salmonella spp., and shigellosis disease-causing agents (shigella spp. and enteroinvasive escherichia coli [eiec]). overall, 143 (13.5%) specimens tested positive by pcr for the targets named above; 3 coinfections and 109 (10.4%) campylobacter spp., 17 (1.6%) salmonella ...201627307458
quantifying potential sources of surface water contamination with campylobacter jejuni and campylobacter coli.campylobacter is the most common causative agent of human bacterial gastroenteritis and is frequently found in surface water, where it indicates recent contamination with animal faeces, sewage effluent, and agricultural run-off. the contribution of different animal reservoirs to surface water contamination with campylobacter is largely unknown. in the netherlands, the massive poultry culling to control the 2003 avian influenza epidemic coincided with a 44-50% reduction in human campylobacteriosi ...201627244295
multilocus sequence typing of campylobacter jejuni and campylobacter coli isolates from poultry, cattle and humans in nigeria.to determine the genetic diversity of campylobacter jejuni and campylobacter coli isolates from nigeria and to identify the association between multilocus sequence types and hosts (poultry, cattle and humans).201627206561
functional genomics in campylobacter coli identified a novel streptomycin resistance gene located in a hypervariable genomic region.numerous aminoglycoside resistance genes have been reported in campylobacter spp. often resembling those from gram-positive bacterial species and located in transferable genetic elements with other resistance genes. we discovered a new streptomycin (str) resistance gene in campylobactercoli showing 27-34 % amino acid identity to aminoglycoside 6-nucleotidyl-transferases described previously in campylobacter. str resistance was verified by gene expression and insertional inactivation. this ant-li ...201627154456
l-fucose influences chemotaxis and biofilm formation in campylobacter jejuni.campylobacter jejuni and campylobacter coli are zoonotic pathogens once considered asaccharolytic, but are now known to encode pathways for glucose and fucose uptake/metabolism. for c. jejuni, strains with the fuc locus possess a competitive advantage in animal colonization models. we demonstrate that this locus is present in > 50% of genome-sequenced strains and is prevalent in livestock-associated isolates of both species. to better understand how these campylobacters sense nutrient availabili ...201627145048
antibiotic resistance and polymorphism in the quinolone resistance-determining region of campylobacter spp. isolated from 1-day-old ducklings.thirty-three isolates of campylobacter coli and three isolates of campylobacter jejuni were recovered from 150 1-day-old ducklings. all isolates were sensitive to chloramphenicol and amikacin, but resistant to sulfamethoxazole-trimethoprim (sxt) by the disc diffusion method. most isolates were susceptible to tetracycline and erythromycin, but resistant to ofloxacin and ciprofloxacin. of the 33 c. coli isolates, nine were positive for the tetracycline resistance gene tet(o), although only two of ...201627068149
combined campylobacter jejuni and campylobacter coli rapid testing and molecular epidemiology in conventional broiler flocks.campylobacter spp. are important causes of bacterial zoonosis, most often transmitted by contaminated poultry meat. from an epidemiological and risk assessment perspective, further knowledge should be obtained on campylobacter prevalence and genotype distribution in primary production. consequently, 15 austrian broiler flocks were surveyed in summer for their thermophilic campylobacter spp. contamination status. chicken droppings, dust and drinking water samples were collected from each flock at ...201627061601
molecular subtyping and erythromycin resistance of campylobacter in china.to investigate the erythromycin resistance patterns and mechanism for campylobacter isolates in china.201626999516
discriminative power of campylobacter phenotypic and genotypic typing methods.the aim of this study was to compare different typing methods, individually and combined, for use in the monitoring of campylobacter in food. campylobacter jejuni (n=94) and campylobacter coli (n=52) isolated from different broiler meat carcasses were characterized using multilocus sequence typing (mlst), flagellin gene a restriction fragment length polymorphism typing (flaa-rflp), antimicrobial resistance profiling (amrp), the presence/absence of 5 putative virulence genes; and, exclusively for ...201626996762
influence of commercial laying hen housing systems on the incidence and identification of salmonella and campylobacter.the housing of laying hens is important for social, industrial, and regulatory aspects. many studies have compared hen housing systems on the research farm, but few have fully examined commercial housing systems and management strategies. the current study compared hens housed in commercial cage-free aviary, conventional cage, and enriched colony cage systems. environmental and eggshell pool samples were collected from selected cages/segments of the housing systems throughout the production cycl ...201626976901
the effects of 405-nm visible light on the survival of campylobacter on chicken skin and stainless steel.campylobacter spp. are foodborne pathogens responsible for a significant portion of human cases of bacterial-mediated gastrointestinal disease. a primary method for the introduction of campylobacter into the food supply is through poultry products. reducing the number of campylobacter on poultry products may reduce the incidence of human disease. research has been conducted on the use of light to inactivate campylobacter on poultry products and processing environments. more recently, the use of ...201626938455
isolation, identification and antibiotic resistance of campylobacter strains isolated from domestic and free-living pigeons.1. the aim of this study was to evaluate the occurrence of campylobacter spp. in domestic and free-living pigeons and to evaluate the antibiotic resistance profiles. 2. the material consisted of cloacal swabs obtained from 108 homing pigeons and fresh faeces from 72 wild birds from lublin and its vicinity. the identification of strains isolated on differential/selective media for campylobacter spp. was carried out by maldi-tof and pcr. the susceptibility to antibiotics was evaluated by minimum i ...201626841300
characterization of antimicrobial susceptibility and its association with virulence genes related to adherence, invasion, and cytotoxicity in campylobacter jejuni and campylobacter coli isolates from animals, meat, and humans.the aim of this research was to statistically analyze the association between antimicrobial susceptibility/resistance to erythromycine, gentamicin, ciprofloxacin, and tetracycline and 11 virulence genes associated with adherence, invasion, and cytotoxicity in 528 isolates of campylobacter coli and campylobacter jejuni obtained from retail meat and fecal samples from food-producing animals and human patients. a high percentage of campylobacter strains were resistant to antimicrobials, specificall ...201626779841
zoonotic public health hazards in backyard chickens.backyard poultry has become increasingly popular in industrialized countries. in addition to keeping chickens for eggs and meat, owners often treat the birds as pets. however, several pathogenic enteric bacteria have the potential for zoonotic transmission from poultry to humans but very little is known about the occurrence of zoonotic pathogens in backyard flocks. the occurrence and the antimicrobial resistance of salmonella enterica, campylobacter spp., listeria monocytogenes and enteropathoge ...201626752227
prevalence and antimicrobial resistance of campylobacterjejuni and campylobacter coli from adult hospitalized patients with diarrhea in thailand.infection with campylobacter jejuni and c. coli are recognized as the major cause of bacterial gastroenteritis in humans.201526749592
campylobacteriosis: the role of poultry meat.the incidence of human infections caused by campylobacter jejuni and campylobacter coli, the main bacterial agents of gastrointestinal disease, has been increasing worldwide. here, we review the role of poultry as a source and reservoir for campylobacter. contamination and subsequent colonization of broiler flocks at the farm level often lead to transmission of campylobacter along the poultry production chain and contamination of poultry meat at retail. yet campylobacter prevalence in poultry, a ...201626686808
salmonella and campylobacter: antimicrobial resistance and bacteriophage control in poultry.salmonella and campylobacter are major causes of foodborne related illness and are traditionally associated with consuming undercooked poultry and/or consuming products that have been cross contaminated with raw poultry. many of the isolated salmonella and campylobacter that can cause disease have displayed antimicrobial resistance phenotypes. although poultry producers have reduced on-the-farm overuse of antimicrobials, antimicrobial resistant salmonella and campylobacter strains still persist. ...201626678136
identification of the main quinolone resistance determinant in campylobacter jejuni and campylobacter coli by mama-deg pcr.among zoonotic diseases, campylobacteriosis stands out as the major bacterial infection producing human gastroenteritis. antimicrobial therapy, only recommended in critical cases, is challenged by resistance mechanisms that should be unambiguously detected for achievement of effective treatments. quinolone (ciprofloxacin) resistance of campylobacter jejuni and campylobacter coli, the 2 main campylobacter detected in humans, is conferred by the mutation gyra c-257-t, which can be genotyped by sev ...201626658311
molecular characterization, antimicrobial resistance and caco-2 cell invasion potential of campylobacter jejuni/coli from young children with diarrhea.campylobacter is a major cause of bacterial gastroenteritis worldwide. young children represent a particular age group affected by campylobacter infection because of their limited diets and weak immune systems.201626627197
description of an erm(b)-carrying campylobacter coli isolate in europe. 201626604242
comprehensive analysis of flagellin glycosylation in campylobacter jejuni nctc 11168 reveals incorporation of legionaminic acid and its importance for host colonization.campylobacter jejuni is the leading cause of bacterial gastroenteritis. it relies on several virulence factors for host colonization, including glycosylated flagella. c. jejuni nctc 11168 modifies its flagellins with pseudaminic acid derivatives. it is also presumed to modify these proteins with legionaminic acid, although no glycopeptide evidence was available at the onset of this study. the enzyme encoded by cj1319 can be used to make legionaminic acid in vitro, but the pathway for legionamini ...201626582606
effect of antimicrobial use in agricultural animals on drug-resistant foodborne campylobacteriosis in humans: a systematic literature review.controversy continues concerning antimicrobial use in food animals and its relationship to drug-resistant infections in humans. we systematically reviewed published literature for evidence of a relationship between antimicrobial use in agricultural animals and drug-resistant foodborne campylobacteriosis in humans. based on publications from the united states (u.s.), canada and denmark from 2010 to july 2014, 195 articles were retained for abstract review, 50 met study criteria for full article r ...201626580432
species shift and multidrug resistance of campylobacter from chicken and swine, china, 2008-14.the objective of this study was to investigate the prevalence and antimicrobial resistance of campylobacter isolated from broiler chickens and swine during 2008-14.201626568567
[antimicrobial susceptibility and resistance mutations in campylobacter jejuni and c. coli isolates from human and meat sources].recently, there has been a marked increase in the number of reports of fluoroquinolone-resistant campylobacter jejuni and campylobacter coli. the aim of this study was to evaluate the prevalence of antimicrobial resistance and its genetic determinants in campylobacter species isolated from meat and human subjects in fukuoka prefecture, japan. between 2011 and 2013, 55 and 64 isolates were collected from meat (chicken meat and beef liver) and humans, respectively, in this prefecture. antimicrobia ...201526552121
genotyping of campylobacter coli strains isolated in brazil suggests possible contamination amongst environmental, human, animal and food sources.campylobacter coli and campylobacter jejuni are two of the most common causative agents of food-borne gastroenteritis in numerous countries worldwide. in brazil, campylobacteriosis is under diagnosed and under-reported, and few studies have molecularly characterized campylobacter spp. in this country. the current study genotyped 63 c. coli strains isolated from humans (n512), animals (n521), food (n510) and the environment (n520) between 1995 and 2011 in brazil. the strains were genotyped using ...201626531157
eucast recommendations for antimicrobial susceptibility testing applied to the three main campylobacter species isolated in humans.antimicrobial susceptibility testing of campylobacter isolates is of great importance for treatment options especially in systemic diseases. the european committee for antimicrobial susceptibility testing (eucast) recently proposed epidemiological cut-offs (ecoffs) for a limited number of antimicrobial compounds and for campylobacter jejuni and campylobacter coli only. in the present study, the eucast method was used after minor modifications to define antimicrobial susceptibility patterns for, ...201526519770
efficacy of a typing scheme for campylobacter based on the combination of true and questionable crispr.this study evaluates an improved scheme for campylobacter genotyping based on the combination of true and questionable crispr (clustered regularly interspaced short palindromic repeats) elements. a total of 180 campylobacter strains (campylobacter jejuni n=93 and campylobacter coli n=87), isolated from neck skin and caecal content of broilers, poultry meat and sewage water were analysed. another 97 c. jejuni dna samples from cases of human campylobacteriosis were assessed. sixty-three genotypes ...201526518609
campylobacter in poultry: ecology and potential interventions.avian hosts constitute a natural reservoir for thermophilic campylobacter species, primarily campylobacter jejuni and campylobacter coli, and poultry flocks are frequently colonized in the intestinal tract with high numbers of the organisms. prevalence rates in poultry, especially in slaughter-age broiler flocks, could reach as high as 100% on some farms. despite the extensive colonization, campylobacter is essentially a commensal in birds, although limited evidence has implicated the organism a ...201526473668
campylobacter species in animal, food, and environmental sources, and relevant testing programs in canada.campylobacter species, particularly thermophilic campylobacters, have emerged as a leading cause of human foodborne gastroenteritis worldwide, with campylobacter jejuni, campylobacter coli, and campylobacter lari responsible for the majority of human infections. although most cases of campylobacteriosis are self-limiting, campylobacteriosis represents a significant public health burden. human illness caused by infection with campylobacters has been reported across canada since the early 1970s. m ...201526422448
prevalence and antimicrobial resistance of campylobacter jejuni and campylobacter coli isolated from children and environmental sources in urban and suburban areas.campylobacteriosis is a dominant bacterial cause of foodborne infection and is considered the main public health problem in europe and many other countries worldwide. in the study lasting from 2011 to 2013 we compared the prevalence and antimicrobial resistance of campylobacter jejuni and campylobacter coli isolated from children, domestic animals, poultry meat and surface water in northern poland.201728376713
multiplex pcr assay for identifi cation and differentiation of campylobacter jejuni and campylobacter coli isolates.campylobacter spp. are important causative agents of gastrointestinal infections in humans. the most frequently isolated strains of this bacterial genus are campylobacter jejuni and campylobacter coli. to date, genetic methods for bacterial identification have not been used in bulgaria. we optimized the multiplex psr assay to identify campylobacter spp. and differentiate c. jejuni from c. coli in clinical isolates. we also compared this method with the routinely used biochemical methods.201727552785
large sequence diversity within the biosynthesis locus and common biochemical features of campylobacter coli lipooligosaccharides.despite the importance of lipooligosaccharides (loss) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of los in campylobacter coli in this study, we investigated the distribution of los locus classes among a large collection of unrelated c. coli isolates sampled from several different host species. furthermore, we paired c. coli genomic information and los chemical composition for the first time to investigate possible associations between l ...201627481928
risk of escherichia coli o157:h7, non-o157 shiga toxin-producing escherichia coli, and campylobacter spp. in food animals and their products in qatar.escherichia coli o157:h7, non-o157 e. coli, and campylobacter spp. are among the top-ranked pathogens that threaten the safety of food supply systems around the world. the associated risks and predisposing factors were investigated in a dynamic animal population using a repeat-cross-sectional study design. animal and environmental samples were collected from dairy and camel farms, chicken processing plants, and abattoirs and analyzed for the presence of these pathogens using a combination of bac ...201526408129
antimicrobial susceptibilities of campylobacter jejuni and campylobacter coli recovered from organic turkey farms in germany.the popularity of food produced from animals kept under an organic regimen has increased in recent years. in germany, turkey meat consumption has increased. despite several studies assessing the susceptibility of campylobacters to various antibiotics in poultry, no sufficient data exists regarding the antimicrobial resistance of campylobacters in organic-reared turkeys. this study provides information about antibiotic resistance in campylobacter isolated from turkeys reared on organic farms in g ...201526371330
campylobacter coli infection causing second trimester intrauterine growth restriction (iugr): a case report and review of the literature.campylobacter is a gram-negative, microaerophilic, curved rod and a normal resident of the gastrointestinal flora and may be the cause of disease in animals. transmission to humans occurs by ingestion of contaminated food or by direct contact with infected animals. in the past few decades, an increasing number of reports have implicated the presence of this organism in human abortions as well. an infectious mechanism due to primary placental inflammatory damage followed by secondary damage to th ...201526340229
antibiotic susceptibility of clinical isolates of campylobacter jejuni and campylobacter coli in ontario, canada during 2011-2013.a total of 219 clinical isolates of campylobacter spp. including 180 campylobacter jejuni and 39 campylobacter coli were assessed for in vitro antimicrobial susceptibility. resistance among c. coli was higher for ciprofloxacin (41% versus 30.80%), erythromycin (12.80% versus 3.90%) and lower for tetracycline (53.80% versus 64.60%) compared to c. jejuni.201526320936
development of a novel chromogenic medium for improved campylobacter detection from poultry samples.the presence of expanded-spectrum β-lactamase (esbl)-producing escherichia coli is a common problem in the isolation of campylobacter from poultry samples using conventional cefoperazone-based selective media. a novel chromogenic medium (cm-ht), based on modified charcoal cefoperazone deoxycholate agar (mccda), has been developed as a solution for improved campylobacter detection from poultry samples. although the basic components of cm-ht are the same as mccda, cm-ht uses both granular charcoal ...201526319731
are campylobacters now capable of carbo-loading?campylobacters are a leading cause of gastrointestinal morbidity worldwide and the majority of human infections are triggered by eating foods contaminated with campylobacter jejuni or campylobacter coli. campylobacters are equally notorious for their ability to mimic human glycoconjugate structures and for their capacity to synthesize both n- and o-linked glycoproteins. these species were once considered to be asaccharolytic, but it was recently shown that several strains possess a pathway for f ...201526259768
a transferable plasticity region in campylobacter coli allows isolates of an otherwise non-glycolytic food-borne pathogen to catabolize glucose.thermophilic campylobacter species colonize the intestine of agricultural and domestic animals commensally but cause severe gastroenteritis in humans. in contrast to other enteropathogenic bacteria, campylobacter has been considered to be non-glycolytic, a metabolic property originally used for their taxonomic classification. contrary to this dogma, we demonstrate that several campylobacter coli strains are able to utilize glucose as a growth substrate. isotopologue profiling experiments with (1 ...201526259566
high prevalence and genetic diversity of campylobacter jejuni in wild crows and pigeons.the occurrence, seasonal variation and genetic diversity of campylobacter spp. in pigeons and crows over a 1-year period were evaluated. campylobacter spp. were isolated from 166 (34.6 %) out of 480 wild bird faecal samples. the occurrence of campylobacter spp. in faecal samples was higher among crows (39.2 %) than pigeons (30.0 %), (p < 0.05). campylobacter jejuni was the most common species detected among wild bird faecal samples (98.2 %). meanwhile, campylobacter coli prevalence in wild bird ...201526228635
a new variant of cytolethal distending toxin in a clinical isolate of campylobacter hyointestinalis.increasing numbers of campylobacter hyointestinalis have been isolated from humans and animals with gastroenteritis, although the virulence mechanism of this species remains largely unknown. here, we show that c. hyointestinalis isolated from a patient with diarrhoea in thailand produced a novel variant of cytolethal distending toxin (cdt). sequencing of a 13 965 bp genomic region of c. hyointestinalis carrying the genes coding for ch-cdt revealed three orfs of 798, 804 and 537 bp, which code fo ...201526220191
adherence reduction of campylobacter jejuni and campylobacter coli strains to hep-2 cells by mannan oligosaccharides and a high-molecular-weight component of cranberry extract.campylobacter infections are a leading cause of human bacterial gastroenteritis in the united states and are a major cause of diarrheal disease throughout the world. colonization and subsequent infection and invasion of campylobacter require that the bacteria adhere to the surface of host cells. agents that inhibit adherence could be used prophylactically to reduce campylobacter carriage and infection. mannan oligosaccharides (mos) have been used as a feed supplement in livestock animals to impr ...201526219363
microarray on digital versatile disc for identification and genotyping of salmonella and campylobacter in meat products.highly portable, cost-effective, and rapid-response devices are required for the subtyping of the most frequent food-borne bacteria; thereby the sample rejection strategies and hygienization techniques along the food chain can be tailor-designed. here, a novel biosensor is presented for the generic detection of salmonella and campylobacter and the discrimination between their most prevalent serovars (salmonella enteritidis, salmonella typhimurium) and species (campylobacter jejuni, campylobacter ...201526198111
rapid detection of campylobacter jejuni, campylobacter coli, and campylobacter lari in fresh chicken meat and by-products in bangkok, thailand, using modified multiplex pcr.a multiplex pcr assay for simultaneous detection and differentiation of campylobacter jejuni, campylobacter coli, and campylobacter lari was developed and validated to assess the occurrence of these bacteria in fresh chicken meat and by-products in bangkok, thailand, by using a new combination of four previously published pcr primers for c. jejuni, c. coli, c. lari, and a universal 16s rdna gene as an internal control. the specificity was determined by using 13 strains of other bacteria. with pu ...201526197289
real-time pcr detection of campylobacter spp.: a comparison to classic culturing and enrichment.the major disadvantage of the current gold standard for detection of the food pathogen campylobacter, i.e. culturing, is the lengthy procedure. in this study we assessed the use of real-time pcr for detection of campylobacter. to this end, 926 poultry samples, taken from transport containers and broiler caeca in the netherlands in 2007, were subjected to three different real-time pcr detection methods: one targeting the campylobacter jejuni hipo gene, one targeting the campylobacter coli glya ge ...201526187833
restoring the selectivity of modified charcoal cefoperazone deoxycholate agar for the isolation of campylobacter species using tazobactam, a β-lactamase inhibitor.extended spectrum β-lactamase (esbl) producing escherichia coli have emerged as a contaminant on modified charcoal cefoperazone deoxycholate agar (mccda) when attempting to selectively isolate campylobacter spp. from poultry. e. coli are particularly problematic given their ability to grow under microaerophilic conditions and have been shown to outcompete campylobacter species making campylobacter detection or enumeration difficult. this paper recommends a novel method for restoring the selectiv ...201526119190
the evolution of campylobacter jejuni and campylobacter coli.the global significance of campylobacter jejuni and campylobacter coli as gastrointestinal human pathogens has motivated numerous studies to characterize their population biology and evolution. these bacteria are a common component of the intestinal microbiota of numerous bird and mammal species and cause disease in humans, typically via consumption of contaminated meat products, especially poultry meat. sequence-based molecular typing methods, such as multilocus sequence typing (mlst) and whole ...201526101080
comparative analysis of antimicrobial resistance and genetic diversity of campylobacter from broilers slaughtered in poland.in the current study, the relationship of campylobacter jejuni and campylobacter coli strains isolated at slaughter was investigated using comparative analysis of antimicrobial resistance (amr), virulence gene (vg) and pfge profiling. a total of 254 campylobacter isolates from poultry caeca and corresponding carcasses, including 139 c. jejuni and 115 c. coli strains were tested. the most prevalent resistance profiles observed in c. jejuni were ciprofloxacin, nalidixic acid and tetracycline (46 o ...201526092707
microbiological survey of birds of prey pellets.a microbiological survey of 73 pellets collected from different birds of prey species housed at the wildlife rescue and rehabilitation center of napoli (southern italy) was performed. pellets were analyzed by culture and biochemical methods as well as by serotyping and polymerase chain reaction. we isolated a wide range of bacteria some of them also pathogens for humans (i.e. salmonella enterica serotype typhimurium, campylobacter coli, escherichia coli o serogroups). this study highlights the p ...201526026881
prevalence of campylobacter spp. in retail chicken, turkey, pork, and beef meat in poland between 2009 and 2013.the purpose of the present study was to determine the prevalence of thermophilic campylobacter in poultry, pork, and beef meat at the retail level and to identify the main categories of meat representing the most significant reservoirs of campylobacter. a monitoring study was conducted throughout poland from 2009 to 2013. a total of 1,700 fresh meat samples were collected from supermarkets, large retail outlets, and smaller stores. thermophilic campylobacter species were detected in 690 (49.3%) ...201525951401
development of transient phage resistance in campylobacter coli against the group ii phage cp84.recently, there is a growing interest in the use of bacteriophages for pre- and post-harvest applications to reduce foodborne pathogens (including campylobacter) along the food chain. quantitative campylobacter reductions of up to three log10 units have been achieved by phage application. however, possible phage resistance might limit this approach. in campylobacter (c.) jejuni, phage resistance mechanisms have been described in detail but data on these mechanisms in c. coli are still missing. t ...201525876274
a five-year study on prevalence and antimicrobial resistance of campylobacter from poultry carcasses in poland.during 2009-2013 a total of 2114 swab samples collected from broiler carcasses in all 16 voivodeships (administrative districts) of poland were examined for the presence of campylobacter jejuni and campylobacter coli. the antimicrobial resistance of the isolates to ciprofloxacin, tetracycline and erythromycin using the mic method was also tested. it was found that 1151 (54.4%) carcasses were contaminated with campylobacter, with 50% of c. jejuni and c. coli species isolated from positive samples ...201525846926
effect of exposure to stress conditions on propidium monoazide (pma)-qpcr based campylobacter enumeration in broiler carcass rinses.campylobacter quantification by qpcr is unable to distinguish viable vs. dead cells in contrast to the culture-based iso 10272-2 reference method. propidium monoazide (pma) has been used to overcome this disadvantage. a campylobacter pma-qpcr enumeration method was evaluated for its consistency and compared to the culture-based enumeration for both artificially and natural contaminated broiler carcass rinses. the pma effect was further evaluated on stressed cells. five conditions, commonly encou ...201525791007
higher resistance of campylobacter coli compared to campylobacter jejuni at chicken slaughterhouse.in order to compare the prevalence of campylobacter coli and campylobacter jejuni during the processing of broilers at slaughterhouse a total of 848 samples were analyzed during 2012 in southern spain. four hundred and seventy six samples were collected from cloaca, carcass surfaces and quartered carcasses. moreover, 372 environmental swabs from equipment and scalding water were collected. minimum inhibitory concentration (mic) to ciprofloxacin, erythromycin, streptomycin, tetracycline and genta ...201525770597
characteristics of metroxylon sagu resistant starch type iii as prebiotic substance.resistant starch type iii (rs3 ) was produced from sago (metroxylon sagu) and evaluated for its characteristics as a prebiotic. two rs3 samples designated sago rs and hcl-sago rs contained 35.71% and 68.30% rs, respectively, were subjected to hydrolyses by gastric juice and digestive enzymes and to absorption. both sago rs and hcl-sago rs were resistant to 180 min hydrolysis by gastric acidity at ph 1 to 4 with less than 0.85% hydrolyzed. both samples were also resistant toward hydrolysis by gas ...201525739421
identification of campylobacter species isolates with phenotypic methods and polymerase chain reaction.campylobacter jejuni (c. jejuni) and campylobacter coli (c. coli) are the most common bacterial causes of enterocolitis in humans. however, identification of the species level is not always possible using standard biochemical tests.201525731001
survival after cryogenic freezing of campylobacter species in ground turkey patties treated with polyphosphates.the use of polyphosphate-based marinades in the processing of poultry has been previously shown to increase the survival of campylobacter species present in the exudates derived from these products. this study investigates the effects that some of the same polyphosphates have on the survival of campylobacter species within a ground turkey product subjected to cryogenic freezing. ground turkey patties with two different polyphosphate formulations added in two different concentrations were artific ...201525710161
antimicrobial resistance in campylobacter coli and campylobacter jejuni in cynomolgus monkeys (macaca fascicularis) and eradication regimens.campylobacter spp. are zoonotic pathogens, however, knowledge about their presence and antimicrobial resistance in nonhuman primates is limited. our animal facility purchased cynomolgus monkeys (macaca fascicularis) from various asian countries: china, cambodia, indonesia, the philippines, and vietnam.201725683191
novel gentamicin resistance genes in campylobacter isolated from humans and retail meats in the usa.to understand the molecular epidemiology of gentamicin-resistant campylobacter and investigate aminoglycoside resistance mechanisms.201525645207
rapid identification and classification of campylobacter spp. using laser optical scattering technology.campylobacter jejuni and campylobacter coli are the two important species responsible for most of the campylobacter infections in humans. reliable isolation and detection of campylobacter spp. from food samples are challenging due to the interferences from complex food substances and the fastidious growth requirements of this organism. in this study, a novel biosensor-based detection called bardot (bacterial rapid detection using optical scattering technology) was developed for high-throughput s ...201525583335
[enumeration and antimicrobial resistance of campylobacter species from retail chicken carcasses].to determine campylobacter contamination level and antimicrobial resistance patterns from chicken carcasses in supermarkets and farmer's markets of 9 districts in beijing.201425573127
seasonality and antibiotic resistance of campylobacter in turkish chicken meat.this study investigated the seasonal prevalence and the antimicrobial susceptibility of campylobacter jejuni and campylobacter coli in 264 samples of chicken meat. the samples encompassed wings (n=88), breasts (n=79) and thighs (n=97) and were purchased from different butcheries and markets in elazig province, in eastern turkey, between december 2009 and november 2010. the meat samples were tested for campylobacter presence and the collected isolates were identified as campylobacter jejuni and c ...201425546065
genotypes and antibiotic resistance of bovine campylobacter and their contribution to human campylobacteriosis.campylobacter jejuni and campylobacter coli are the most important bacterial causes of human gastroenteritis. chicken has been recognized as a major source for human infection, whereas cattle might also contribute to a lesser extent. however, there is a paucity of information available regarding campylobacter in swiss cattle and their role for human campylobacteriosis. to gain more information on genotypes and antibiotic resistance of bovine c. jejuni and c. coli and on their contribution to hum ...201525511436
antimicrobial activity of gallic acid against thermophilic campylobacter is strain specific and associated with a loss of calcium ions.gallic acid has been suggested as a potential antimicrobial for the control of campylobacter but its effectiveness is poorly studied. the minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) of gallic acid against campylobacter jejuni (n = 8) and campylobacter coli (n = 4) strains was determined. gallic acid inhibited the growth of five c. jejuni strains and three c. coli strains (mic: 15.63-250 μg ml(-1)). gallic acid was only bactericidal to two c. coli strains ( ...201525475290
comparison of anti-campylobacter activity of free thymol and thymol-β-d-glucopyranoside in absence or presence of β-glycoside-hydrolysing gut bacteria.thymol is a natural product that exhibits antimicrobial activity in vitro but in vivo results indicate that absorption within the proximal alimentary tract precludes its delivery to the distal gut. presently, the anti-campylobacter activity of thymol was compared against that of thymol-β-d-glucopyranoside, the latter being resistant to absorption. when treated with 1 mm thymol, campylobacter coli and jejuni were reduced during pure or co-culture with a β-glycoside-hydrolysing parabacteroides dis ...201525465999
prevalence, virulence, and antimicrobial resistance of campylobacter jejuni and campylobacter coli in white stork ciconia ciconia in poland.the aim of this study was to investigate the role of white stork ciconia ciconia as a potential reservoir of campylobacter spp. antimicrobial resistance and the presence of putative virulence genes of the isolates were also examined. a total of 398 white stork chicks sampled in western poland in habitats with high density of breeding were examined. rectal swabs were collected during breeding season 2009-2012 from storks developing in a relatively pure environment (odra meadows), in polluted area ...201525456607
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