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spontaneous bacterial peritonitis due to campylobacter coli.a patient with alcoholic liver disease and ascites who developed fatal spontaneous bacterial peritonitis due to campylobacter coli is reported herein. this is the first case report of spontaneous bacterial peritonitis due to this organism. campylobacter infections and their relationship to spontaneous bacterial peritonitis are reviewed.19873569775
demonstration of a flagellar antigen shared by a diverse group of spiral-shaped bacteria that colonize intestinal mucus.western blot analysis showed that there is little immunological cross-reactivity of the human pathogens campylobacter jejuni and campylobacter coli with campylobacter pyloridis, except for a very strong cross-reaction between the flagellins. this same antigenic cross-reaction was found with two isolates of gram-negative spiral microaerophilic bacteria that are known to colonize the intestinal mucosa of rodents, but not with the flagellins of a number of other motile bacteria. it is proposed that ...19873818099
erythromycin-resistant campylobacter infections in thailand.erythromycin therapy was compared with no treatment in a prospective trial of acute diarrheal disease among 100 infants in an orphanage in bangkok. within 24 h of the onset of diarrhea, 50 children received erythromycin ethylsuccinate (40 mg/kg per day) in four divided doses for 5 days. campylobacter jejuni isolated from 31, campylobacter coli isolated from 21, and shigella spp. isolated from 21 of 100 children were the most commonly recognized pathogens; use of a sensitive, nonselective method ...19873579261
seasonal occurrence of campylobacter spp. in surface waters and their correlation with standard indicator bacteria.campylobacter jejuni, campylobacter coli, and a campylobacter-like organism were isolated from a number of natural water sources in central washington, including ponds, lakes, and small mountain streams at elevations ranging from 1,460 to 5,400 feet (ca. 445 to 1,646 m) above sea level. at the two sites where extensive sampling was done, the bacteria were recovered throughout the year. generally, the recovery rates were highest in the fall and winter months and lowest during the spring and summe ...19873579268
[the epidemiology of campylobacter jejuni and campylobacter coli in the fattening chicken]. 19873589638
atypical campylobacters associated with gastroenteritis.nine strains of campylobacter species other than campylobacter jejuni, campylobacter coli, and campylobacter laridis were isolated from patients with acute diarrhea. all nine strains showed preferred growth at 37 degrees c under microaerophilic conditions. conventional microbiological tests and dna-dna dot blotting were used to identify these strains. three of the nine campylobacter strains hydrolyzed hippurate, reduced nitrate, produced catalase, were resistant to cephalothin, and were shown to ...19873611317
effect of clavulanic acid on susceptibility of campylobacter jejuni and campylobacter coli to eight beta-lactam antibiotics.the effect of clavulanic acid on the susceptibility of 32 strains of campylobacter jejuni and campylobacter coli to eight beta-lactam agents was studied. almost all strains tested became susceptible to amoxicillin and ticarcillin with 1 microgram of clavulanic acid per ml. this compound had little or no effect on susceptibility to penicillin g, cephalothin, cefamandole, and cefoxitin. clavulanic acid had a marginal effect on cefotaxime and moxalactam susceptibility.19873619428
utilization of monoclonal antibodies to evaluate the involvement of campylobacter jejuni in proliferative ileitis in syrian hamsters (mesocricetis auratus).the role of campylobacter jejuni in the pathogenesis of proliferative ileitis in syrian hamsters was evaluated with monoclonal antibodies of different specificities. monoclonal antibodies were produced with two different specificities: one for all members of the genus campylobacter tested (antibody 8322-2e6) and one for c. jejuni and campylobacter coli (antibodies 841-2a11, 841-4c6, and 841-5b1). heal sections from healthy hamsters, from hamsters with naturally occurring proliferative ileitis, a ...19873623701
campylobacter jejuni/campylobacter coli-associated guillain-barré syndrome. immunoblot confirmation of the serological response.immunoblot (western blot) examination of the sera of 45 patients with the guillain-barré syndrome demonstrated that between three and five immunoreactive bands that were characteristic of a recent campylobacter jejuni/campylobacter coli infection were present in 22 of these patients. the immunoblots paralleled the serological response that is found in campylobacter enteritis and confirmed the specificity of our previous enzyme-linked immunosorbent assays (elisas), which indicates that antecedent ...19873626926
in vitro susceptibility of campylobacter jejuni and campylobacter coli isolated in denmark to fourteen antimicrobial agents.the in vitro susceptibility of 95 recent danish human clinical isolates of campylobacter jejuni and campylobacter coli to 14 antimicrobial agents was determined by an agar dilution technique. c. jejuni and c. coli were considered together. doxycycline, ciprofloxacin, gentamicin, clindamycin, nitrofurantoin and erythromycin were the most active drugs. 2% of the strains tested were resistant to erythromycin (mic greater than 3.1 micrograms/ml). therefore susceptibility testing is suggested if anti ...19873630712
evaluation of gamma radiation levels for reducing pathogenic bacteria and fungi in animal sewage and laboratory effluents.sewage samples collected from animal wastes and from effluents at an animal disease laboratory were inoculated with known numbers of pathogenic organisms and subjected to various doses of gamma radiation from a 60co source. surviving test organisms were quantitatively determined by selective and enrichment techniques. the experiment was modeled as a quantal assay in which probit analysis was applied to obtain d10 values. the d10 value represents the irradiating dose required to reduce the popula ...19873651881
naturally occurring auxotrophs of campylobacter jejuni and campylobacter coli.the nutritional requirements for 439 campylobacter jejuni isolates and 46 campylobacter coli isolates were determined by using a previously described chemically defined medium, campylobacter defined medium. with this medium, 45% of both human and nonhuman c. jejuni isolates demonstrated auxotrophic requirements. none of the 46 c. coli isolates studied demonstrated requirements for amino acids on campylobacter defined medium. the most common auxotrophic requirement among c. jejuni isolates was fo ...19873654939
rapid and improved gas-liquid chromatography technique for detection of hippurate hydrolysis by campylobacter jejuni and campylobacter coli.a gas-liquid chromatographic method which requires no chloroform extraction of the split products has been investigated for the detection of hippurate hydrolysis by campylobacter spp. this technique gave better reproducibility than other tests also used in this study and allows the routine use of the gas-liquid chromatographic method for identification of campylobacter isolates.19873654950
antimicrobial resistance of campylobacter jejuni and campylobacter coli with special reference to plasmid profiles of japanese clinical isolates.a total of 111 clinical isolates of campylobacter jejuni and 10 clinical isolates of campylobacter coli were characterized by their susceptibility to nine antimicrobial agents and by their plasmid profiles on agarose gel electrophoresis. all of the c. jejuni isolates were susceptible to chloramphenicol, ciprofloxacin, erythromycin, kanamycin, and nalidixic acid, but 55% were tetracycline resistant. in the 10 c. coli isolates, a high prevalence of multiple-antibiotic resistance was noted. plasmid ...19873038005
nucleotide sequence of the kanamycin resistance determinant of the pneumococcal transposon tn1545: evolutionary relationships and transcriptional analysis of apha-3 genes.the nucleotide sequence of the kanamycin resistance determinant apha-3 encoded by transposon tn1545 from streptococcus pneumoniae was determined and compared to those of plasmids pjh1 and pip1433 from streptococcus faecalis and campylobacter coli, respectively. the three sequences were found to be identical and differed by two substitutions and the deletion of a codon from that of plasmid psh2 from staphylococcus aureus. comparison of the 5' noncoding sequences indicated that the regions contain ...19873039302
substrate utilization by campylobacter jejuni and campylobacter coli.an attempt was made to elucidate in campylobacter spp. some of the physiologic characteristics that are reflected in the kinetics of co2 formation from four 14c-labeled substrates. campylobacter jejuni and c. coli were grown in a biphasic medium, and highly motile spiral cells were harvested at 12 h. of the media evaluated for use in the metabolic tests, minimal essential medium without glutamine, diluted with an equal volume of potassium sodium phosphate buffer (ph 7.2), provided the greatest s ...19862877615
distribution and serotypes of campylobacter jejuni and campylobacter coli in enteric campylobacter strains isolated from children in the central african republic.one hundred eighty-five enteric campylobacter strains isolated from diarrheic or healthy children in bangui (central african republic) were studied to determine their species and serotypes. c. coli was identified in 38.9% of all strains and in 43.9% of strains from diarrheic children. by the hemagglutination technique for heat-stable antigens, 73.5% of the strains could be serotyped. of the typeable strains, 75% were distributed among 13 more frequent serotypes. c. coli serotype pen 37,56 was th ...19863958146
bacteria in enteric lesions of horses.thirty-three species of bacteria were isolated from the gastrointestinal mucosa of 23 adult horses and two foals. the bacteria isolated could be related to gross and microscopical lesions in some cases. clostridium perfringens type a, actinobacillus equuli, salmonella typhimurium and campylobacter coli biotype 1 could all be associated with gastrointestinal lesions. c jejuni biotype 1 and aeromonas hydrophila were both recovered in this study and have been identified as causes of enteritis in ho ...19863716108
effect of iron concentration on toxin production in campylobacter jejuni and campylobacter coli.the effect of iron concentrations in culture media on supernatant yields of campylobacter cytotonic toxin (cct) was studied. of the 118 campylobacter spp. strains surveyed, 78.8% produced toxin in brucella broth or in casamino acids--yeast extract (cye) broth. when the iron concentration of cye was increased from 0.44 microgram/ml (7.9 microm) to 0.65 microgram/ml (11.6 microm) by the addition of ferric chloride, 94.9% of the strains were positive for toxin in a ganglioside gm1 based, enzyme-lin ...19863719459
campylobacter colitis in ranch mink in ontario.outbreaks of colitis, where campylobacter jejuni and campylobacter coli were the only pathogens isolated occurred in weanling mink (mustella vision) on two commercial mink ranches in ontario. lesions were restricted to the proximal colon and were characterized by multiple 1 mm focal or 1 mm linear erosions/ulcers in the region 2 cm distal to the ileal-colonic junction. histological changes included thickening of the colonic mucosa, inflammatory cell infiltrate in the lamina propria and submucosa ...19863742356
[waste water from poultry coops, a potential source of human infection with campylobacter coli]. 19863749726
strain characterization and grouping of campylobacter jejuni and campylobacter coli by interaction with lectins.strains of campylobacter jejuni and campylobacter coli were characterized and grouped by their distinct reaction patterns with lectins. heating of the campylobacter cultures to 100 degrees c and holding for 30 to 60 min greatly enhanced their reactivity with lectins and permitted the grouping of all but 3 of 155 cultures tested in this study without interference of autoagglutination and other nonspecific activities. the lectin reaction patterns of the heated cultures were stable and reproducible ...19863754264
the chicken embryo as a model for campylobacter invasion: comparative virulence of human isolates of campylobacter jejuni and campylobacter coli.eleven-day-old chicken embryos were used to compare the relative virulence of minimally passaged human isolates of campylobacter jejuni and campylobacter coli. graded doses of bacteria were inoculated onto the chorioallantoic membrane, and 50% lethal doses were calculated at 72 h postinfection. strains varied markedly in their ability to invade the chorioallantoic membrane and kill the embryos. the 50% lethal doses varied by about 6 logs for 25 strains of c. jejuni, and by 2 logs for 5 strains o ...19863759232
association with hela cells of campylobacter jejuni and campylobacter coli isolated from human feces.we developed a rapid in vitro test for determining the association of campylobacter jejuni and c. coli with hela cells. association was expressed as a weighted mean of the number of bacteria associated with one cell in an association index (ai). the reproducibility of the ai was checked by repeating the test six times, using four strains chosen at random. means and standard deviations of the means were 7.3 +/- 1.2, 6.8 +/- 0.9, 1.8 +/- 1.2, and 0.1 +/- 0.2. the experimental conditions for which ...19863770943
isolation and characterization of cephalothin-susceptible campylobacter coli from slaughter cattle.in a recent meat survey, 10 of 13 (77%) campylobacter coli isolates were susceptible to cephalothin. these organisms were isolated from nine slaughter cattle from eight meat packing establishments. all 10 isolates grew at 43 degrees c but not at 25 degrees c, were catalase and oxidase positive, and were susceptible to nalidixic acid (30 micrograms) and cephalothin (30 micrograms). the cultures were subsequently identified as c. coli serogroup 20, biotype i (lior scheme). sodium dodecyl sulfate-p ...19863771747
modified selective medium for isolation of campylobacter spp. from feces: comparison with preston medium, a blood-free medium, and a filtration system.our previously described (h. goossens, m. de boeck, and j. p. butzler, eur. j. clin. microbiol. 2:389-393, 1983) selective medium, consisting of cefoperazone (15 mg/liter), rifampin (10 mg/liter), colistin (10,000 iu/liter), and amphotericin b (2 mg/liter) (medium m1), for the isolation of campylobacter jejuni and campylobacter coli from stool specimens was modified as follows: cefoperazone (30 mg/liter), rifampin (10 mg/liter), and amphotericin b (2 mg/liter) (medium m2). a comparative study of ...19863771769
biotype and serogroup distribution of campylobacter isolates from children in nigeria.a total of 101 campylobacter isolates from nigerian children with or without gastroenteritis were biotyped and serogrouped by using the lior typing schemes (h. lior, j. clin. microbiol. 20:636-640, 1984; h. lior, d. l. woodward, j. a. edgar, l. j. laroche, and p. gill, j. clin. microbiol. 15:761-768, 1982). fifty-three (52.5%) of the isolates were campylobacter jejuni biotype i, 29 (28.7%) were c. jejuni biotype ii, 10 (9.9%) were campylobacter coli biotype i, and 9 (8.9%) were c. coli biotype i ...19863771772
rate of campylobacter spp. isolation in three regions of ontario, canada, from 1978 to 1985.isolation rates of campylobacter spp. were analyzed for an 8-year period, 1978 through 1985. three laboratories of the ontario ministry of health examined 146,842 human feces samples for bacterial pathogens, including campylobacter jejuni and campylobacter coli. campylobacter spp. were isolated from 5,580 specimens (3.8%), with monthly isolation rates ranging from 1.1 to 7.4%. the data showed a seasonal distribution of isolations, with peaks during the summer months (june to september). most inf ...19863771774
monosaccharide composition of lipopolysaccharides from campylobacter jejuni and campylobacter coli.the monosaccharide composition of the lps from 5 campylobacter jejuni strains and 7 campylobacter coli strains has been studied. all lps's contained kdo, heptose, glucosamine, glucose, and (with one exception) galactose. all c. jejuni and 3 c. coli lps's contained greater than 1% galactosamine. 3-amino-3.6-dideoxyglucose was present in all but one c. coli lps and in only one c. jejuni lps.19863772734
[epidemiology and epizootiology of enteritis caused by campylobacter jejuni and campylobacter coli]. 19863822876
prevalence of campylobacter jejuni and campylobacter coli among wild and domestic animals in yamaguchi prefecture. 19863574158
cytotoxic and cytotonic factors produced by campylobacter jejuni, campylobacter coli, and campylobacter laridis.complete toxigenicity studies were performed on 341 strains of campylobacter spp., including 23 nonhuman isolates. toxin profiles based on both cytotonic and cytotoxic factors were determined after analyzing responses in vero, hela, cho and y-1 cells. suckling mouse assays were consistently negative for all culture filtrates tested. toxin-producing strains were frequently encountered among both the human and nonhuman strains of campylobacter jejuni, c. coli, and c. laridis investigated. strains ...19863018039
aminoglycoside-phosphotransferases aph(3')-iv and aph(3") synthesized by a strain of campylobacter coli.campylobacter coli strain 981 of animal origin was resistant to erythromycin, tetracycline, streptomycin, kanamycin, ribostamycin, neomycin, paromomycin, lividomycin, and butirosin. resistance to aminoglycosides of strain 981 was mediated by phosphotransferases aph (3') type-iv and aph (3"). c. coli 981 harboured three plasmids of 24, 34, and 40 megadaltons respectively. none of these plasmids were transferable to escherichia coli k-12 by conjugation.19863019983
evolution and transfer of aminoglycoside resistance genes under natural conditions.3'-aminoglycoside phosphotransferases [aph(3')] were chosen as a model to study the evolution and the transfer of aminoglycoside resistance genes under natural conditions. comparison of the amino acid sequences of aph(3') enzymes from transposons tn903 (type i) and tn5 (type ii) detected in gram-negative bacteria, from the gram-positive staphylococcus and streptococcus (type iii), from the butirosin-producing bacillus circulans (type iv) and from a neomycin-producing streptomyces fradiae (type v ...19863027020
isolation and characterization of a common antigen in campylobacter jejuni and campylobacter coli.flagellin protein was isolated and purified from two serotype reference strains of campylobacter jejuni, pen 1 and pen 3. each preparation was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to consist of a diffuse band with a molecular mass of approximately 62 kilodaltons. antisera were prepared against flagellin from pen 1, and specific antibody was isolated by affinity chromatography with flagellin protein covalently bound to cyanogen bromide-activated sepharose. the high-a ...19862424932
extraintestinal campylobacter jejuni and campylobacter coli infections: host factors and strain characteristics.to determine whether extraintestinal isolates of campylobacter jejuni and campylobacter coli are the consequence of unusual host or bacterial characteristics, we studied clinical and bacteriologic features of 24 extraintestinal infections. common serotypes and auxotypes were present among the extraintestinal isolates. gastrointestinal isolates were more susceptible to normal human serum than were the systemic isolates; however, the ranges overlapped considerably. predispositions to systemic spre ...19863512731
western blot analysis of intestinal secretory immunoglobulin a response to campylobacter jejuni antigens in patients with naturally acquired campylobacter enteritis.secretory immunoglobulin a (siga) response at the intestinal mucosa is a primary defense against enteric infections. we sought to determine which antigens of campylobacter jejuni outer membranes elicited siga responses in 8 patients with naturally acquired campylobacter enteritis using western blot analysis of fecal extracts. naturally acquired campylobacter infection elicited an siga response in 7 of 8 patients. of these 7 patients, 5 had campylobacter-specific siga titers of 1:16 and two had t ...19863514359
two-dimensional thin-layer chromatography for the specific detection of hippurate hydrolysis by microorganisms.glycine, one of the end products of hippurate hydrolysis by microorganisms, was detected by a rapid, specific technique utilizing two-dimensional thin-layer chromatography. a loopful of growth of each organism from its suitable agar medium was washed, suspended, and incubated with 0.1% sodium hippurate for 30 min at 37 degrees c. the supernatant of the incubated suspension from each organism was then dansylated, and the dansyl derivatives were separated by two-dimensional thin-layer chromatograp ...19863517036
abortion and perinatal sepsis associated with campylobacter infection.fetal loss or neonatal sepsis associated with campylobacter infection during pregnancy is infrequently recognized. as reported herein, one case of premature labor and neonatal sepsis due to campylobacter fetus subspecies fetus was treated successfully with ampicillin and gentamicin. only 19 similar cases have been cited in the literature. a review of these 19 cases reveals that the campylobacter species involved were probably c. fetus subspecies fetus in nine instances, campylobacter jejuni in n ...19863523697
methods for detecting and enumerating campylobacter jejuni and campylobacter coli in poultry.methods, media, and biochemical tests for detecting, enumerating, isolating, and identifying campylobacter jejuni and c. coli in foods are summarized with special consideration of poultry and poultry products. information is drawn largely from the american public health association compendium of methods for the microbiological examination of foods and the us food and drug administration bacteriological analytical manual for foods. reference is also made to recently advanced techniques and proced ...19863554203
prevalence of salmonella and campylobacter contamination in manitoba swine carcasses.muscle samples from the neck and diaphragm and fecal samples were collected from five hogs from each of 50 lots of market hogs at three manitoba abattoirs. environmental samples were also collected. each sample was tested for salmonella contamination, and tissue and fecal samples were tested for campylobacter.salmonella was isolated from 34 (4.1%) of 821 samples, with 17 of the isolates coming from one lot of hogs and their concurrent environment. campylobacter was isolated from 175 (26.3%) of 6 ...198617422650
restriction endonuclease digest patterns of chromosomal dna from nitrate-negative campylobacter jejuni-like organisms.high molecular weight chromosomal dna was isolated from ten nitrate-negative campylobacters of human origin (gastric biopsies and faecal specimens). the dna was digested with various restriction endonucleases and the patterns obtained were compared with those of campylobacter coli, c. fetus subsp. fetus, c. jejuni and c. laridis reference strains. electrophoresis of hae iii digests of dna in 0.5% agarose gave excellent patterns which comprised at least 25 well-resolved bands with fragment sizes ...19852876913
interaction of campylobacter jejuni and campylobacter coli with lectins and blood group antibodies.lectins and blood group antibodies were used to probe the surface structures of campylobacter jejuni and campylobacter coli. of the 29 strains tested, there were distinct reaction patterns. the lectin-reactive and blood group antibody-reactive sites on the bacterial surface were distinguishable from the heat-stable (lipopolysaccharide) antigenic determinants. the interactions were strain specific. the reactive sites were stable with respect to culture media and passage and may be useful as addit ...19852410445
survey of plasmids and resistance factors in campylobacter jejuni and campylobacter coli.a total of 688 isolates of campylobacter jejuni and campylobacter coli were screened for the presence of plasmid dna by agarose gel electrophoresis and were tested for susceptibility to ampicillin, chloramphenicol, erythromycin, streptomycin, and tetracycline. of the isolates examined, 32% were noted to harbor plasmid dna, ranging in size from 2.0 to 162 kilobases. only tetracycline resistance was noted to correlate with the presence of plasmids. plasmids capable of transferring tetracycline res ...19852984981
structural relationship between the genes encoding 3'-aminoglycoside phosphotransferases in campylobacter and in gram-positive cocci.campylobacter coli strain bm2509 resistant to ampicillin, chloramphenicol, erythromycin, kanamycin, spectinomycin, streptomycin and tetracycline was isolated from a patient with hospital-acquired diarrhoea. resistance to kanamycin has not been thus far described in campylobacter. phosphocellulose paper-binding assay indicated that resistance to kanamycin and structurally related antibiotics in strain bm2509 was due to synthesis of a 3'-aminoglycoside phosphotransferase of type iii (aph(3')-iii), ...19853002237
comparative in vitro activities of twelve antimicrobial agents against campylobacter species.the in vitro susceptibility of 27 campylobacter jejuni, 31 campylobacter coli, and 30 campylobacter fetus subsp. fetus strains to 12 antimicrobial agents was determined. ciprofloxacin, a new quinoline derivative, was the most active agent tested. antimicrobial susceptibility differed among the three species tested.19853873216
isolation, characterization, and serotyping of campylobacter jejuni and campylobacter coli from slaughter cattle.a total of 525 specimens from 100 slaughter beef cattle were examined for the presence of campylobacter jejuni and campylobacter coli by direct plating and enrichment techniques. isolates were identified by cultural, biochemical, antibiotic sensitivity, and immunofluorescence tests and further characterized with the aid of recently developed biotyping and serotyping methods. fifty animals were positive for c. jejuni; only one was positive for c. coli. the distribution pattern of c. jejuni-positi ...19853888109
in vitro blastogenic response and immunoglobulin synthesis in murine spleen cells stimulated with lipopolysaccharides from campylobacter jejuni and campylobacter coli.lipopolysaccharides isolated from strains of campylobacter jejuni and campylobacter coli were mitogenic for spleen cells as measured by 3h-thymidine incorporation. the incorporation was dose dependent with an increase with increased concentration of lps. addition of lps beyond 500 micrograms/ml gave inhibition of incorporation. stimulation of the spleen cells for 3 days with lps led to a polyclonal activation of immunoglobulin synthesis. the amount of immunoglobulins synthesised showed a maximum ...19853909739
[isolation of campylobacter jejuni and campylobacter coli from intestinal contents and feces of chickens]. 19853925041
in vivo transfer of genetic information between gram-positive and gram-negative bacteria.a 1427-bp dna fragment containing the kanamycin resistance gene, apha-3, of plasmid pip1433 from campylobacter coli was inserted into a shuttle vector. full expression of apha-3 was obtained in bacillus subtilis and in escherichia coli. this dna fragment was sequenced in its entirety and the starting point for apha-3 transcription in b. subtilis, c. coli and e. coli was determined by s1 nuclease mapping. the sequence of the promoter consists of the hexanucleotides ttgaca and tataat, with a spaci ...19853937729
susceptibility of campylobacter isolates to the bactericidal activity of human serum.although campylobacter jejuni and related thermophilic organisms are more common human pathogens than are campylobacter fetus, most bloodstream or systemic isolates are c. fetus. to understand the pathophysiology related to this observation, the authors studied susceptibility to the bactericidal activity of normal human serum of campylobacter coli, c. jejuni, and c. fetus isolates from feces and blood. in standardized assays, 10 of 15 c. jejuni and related isolates showed 90% kill (mean, 90.6% + ...19853968449
comparison of basal media for culturing campylobacter jejuni and campylobacter coli.four strains of campylobacter jejuni and four strains of campylobacter coli were used to compare the quantitative growth of campylobacter cells on blood agar base no. 2 (oxoid), brucella agar (bbl microbiology systems and difco laboratories), campylobacter agar base (difco), columbia blood agar base (difco and oxoid), and mueller-hinton agar (difco and oxoid). columbia blood agar base and blood agar base no. 2 were inhibitory to most of the strains tested, as evidenced by reduced (10- to 1,000-f ...19853972990
[isolation of campylobacter jejuni and campylobacter coli in fecal samples of healthy slaughter swine depending on the season]. 19853976311
in vitro activities of 47 antimicrobial agents against three campylobacter spp. from pigs.the in vitro activities of 47 antimicrobial agents against 30 isolates of campylobacter species from pigs were determined by the agar dilution technique. the isolates were obtained from pigs with proliferative enteritis and included 10 strains each of campylobacter coli, campylobacter sputorum subsp. mucosalis, and "campylobacter hyointestinalis gebhart et al." (this name is not on the approved lists). carbadox, furazolidone, nitrofurantoin, gentamicin, and dimetridazole were the most active dru ...19853985597
use of auxotyping for epidemiological studies of campylobacter jejuni and campylobacter coli infections.a chemically defined medium developed for neisseria gonorrhoeae was modified to support the growth of campylobacter jejuni and campylobacter coli. a total of 76 isolates of c. jejuni and 14 isolates of c. coli were tested on this medium, which was designated campylobacter defined medium (cdm), over a 3-month period. although none of the c. coli isolates appeared to require amino acids, 51% of the c. jejuni tested required one and 7% required multiple amino acids for growth. an analysis of isolat ...19853988340
[season-related incidence of campylobacter jejuni and campylobacter coli in fecal samples of healthy slaughter cattle]. 19853993275
estimation of campylobacter spp. in broth culture by bioluminescence assay of atp.the luciferin-luciferase bioluminescence reaction was used to estimate cell numbers of campylobacter jejuni and campylobacter coli in broth cultures based on a linear relationship between cell numbers (in excess of 10(4) to 10(5] and atp levels. the sensitivity was lower than that obtained with escherichia coli. the calculated amount of intracellular atp per cell of c. jejuni and c. coli ranged from 1.7 to 2.1 fg.19853994377
typing of heat-stable and heat-labile antigens of campylobacter jejuni and campylobacter coli by coagglutination.a coagglutination system has been devised for typing heat-stable and heat-labile antigens of campylobacter jejuni and c. coli. the use of protein a-positive staphylococcus aureus cells carrying campylobacter sp. serotype antibody and the treatment of campylobacter sp. cells with dnase in the antigen suspension permitted rapid and specific coagglutination of rough (autoagglutinable) as well as smooth cultures. cells of s. aureus were sensitized with campylobacter sp. serotype antisera. four to fi ...19853998098
cadmium chloride susceptibility, a characteristic of campylobacter spp.we report a simple diagnostic characteristic useful in the presumptive identification of campylobacter jejuni and campylobacter coli. filter paper disks impregnated with cadmium chloride were placed on streaked agar medium. zones of growth inhibition for campylobacter spp. occurred at 1.25 micrograms per disk. other enteropathogens (salmonella spp., shigella spp., vibrio cholerae, vibrio parahaemolyticus, escherichia coli, and yersinia enterocolitica) were resistant to at least 40 micrograms per ...19853998099
antigenicity of lipopolysaccharides from campylobacter jejuni and campylobacter coli in passive haemagglutination tests and enzyme-linked immunosorbent assays.passive haemagglutination tests and elisa were used to study the serological activity of homologous and heterologous rabbit antisera against lps prepared from various strains of c. jejuni/coli. in both test systems the homologous antisera exhibited serological activity against lps. the heterologous antisera showed some degree of intra- and inter- species cross-reactivity. the cross-reacting was most pronounced in the elisa. erythrocytes sensitized with untreated lps gave higher antibody-titres t ...19854013751
serotyping of campylobacter jejuni, campylobacter coli, and campylobacter laridis from domestic and wild animals.by using 50 unabsorbed antisera, we were able to serotype 272 (65.7%) of 414 thermotolerant campylobacters from wild and domestic animals, on the basis of heat-stable antigens identified by means of passive hemagglutination. forty-two serotypes were recognized. the pattern of serotypes detected in the various animal species was compared to human clinical isolates by using the czekanowski index (proportional similarity index). the highest degree of similarity to the clinical isolates was observed ...19854015088
development of a bacteriophage typing system for campylobacter jejuni and campylobacter coli.a bacteriophage typing system for campylobacter jejuni and campylobacter coli was developed with phages isolated from poultry feces. data for phage selection were generated from a set of isolates of c. jejuni and c. coli from humans in illinois. selection of 14 phages from the 47 phages available was assisted by determination of the sneath-jaccard similarity coefficients and subsequent unweighted pair-group arithmetic averaging cluster analysis. the typing set was reproducible and stable in the ...19854019737
enrichment medium for isolation of campylobacter jejuni-campylobacter coli.a broth enrichment medium for the improvement of isolation of campylobacter jejuni-campylobacter coli from stool samples and other specimens is presented. of 1,228 samples examined in parallel, positive results were obtained from 81 by direct inoculation of selective media and from 112 after enrichment. thus, an increase of 27.7% in the isolation rate was obtained by using the enrichment medium. the same medium without antibiotics allows the preservation of isolates of c. jejuni-c. coli for at l ...19854026287
biotypes and serotypes of campylobacter jejuni and campylobacter coli strains isolated from patients, pigs and chickens in the region of rotterdam.a comparison was made of the distribution of the bio-and serotypes of campylobacter jejuni and campylobacter coli isolated from human patients (205 isolates), intestinal contents of pigs (163 isolates) and intestinal contents and livers of chickens (147 isolates). all strains were isolated in the region of rotterdam. campylobacter jejuni biotype 1 accounted for 86%, 1% and 82% of the human, pig and chicken isolates respectively; c. coli was present in 7%, 98% and 14% respectively. serotyping was ...19854031520
occurrence of plasmids and antibiotic resistance among campylobacter jejuni and campylobacter coli isolated from healthy and diarrheic animals.serologically defined strains of campylobacter jejuni and campylobacter coli from healthy and diarrheic animals were examined for the occurrence of plasmid dna in association with the antibiotic susceptibility of the bacterial host and the health status of the animal host. of all campylobacter organisms surveyed, 53% (116 of 200) contained plasmid dna. a plasmid occurrence rate of 73.8% was obtained for c. coli from healthy pigs, contrasted by lower plasmid occurrence rates for c. coli from diar ...19854044793
[seasonal distribution of campylobacter jejuni and campylobacter coli (1 september 1982--31 august 1983)]. 19854068889
inhibition of campylobacter coli and campylobacter jejuni by antibiotics used in selective growth media.the ability of campylobacter coli and campylobacter jejuni to grow in the presence of antibiotics used in selective growth media was compared. mic data for c. coli indicated that some strains were more susceptible to the antibiotics than were the c. jejuni strains tested. a reduction of greater than 1 log cycle in the numbers of cells growing on plates containing antibiotics was considered to be a marked level of inhibition. only one of nine of the antibiotic combinations studied did not markedl ...19854077962
[occurrence of 3-amino-3,6-dideoxy-d-glucose in a lipopolysaccharide from campylobacter coli]. 19854087160
susceptibility of campylobacter jejuni and campylobacter coli to macrolides and related compounds.the susceptibility of 105 thermophilic campylobacters from human and swine origins to eight macrolides and related compounds was tested. erythromycin, josamycin, clindamycin, and ase 136 bs (a new erythromycin derivative) were the most active against the human strains. the swine strains were highly resistant, except to pristinamycin. the human campylobacter coli strains (except for two strains) behaved like the c. jejuni strains.19854091531
structural and antigenic heterogeneity of lipopolysaccharides of campylobacter jejuni and campylobacter coli.the techniques of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, silver staining, and immunoblotting were used to analyze the lipopolysaccharide (lps) structure of 20 strains of campylobacter jejuni and 4 strains of campylobacter coli belonging to more than 22 thermostable serotypes. the lpss of all strains examined were shown to be of a low-molecular-weight type, and these low-molecular-weight lpss conferred heat-stable serospecificity. high-molecular-weight banding observed with bo ...19846203838
identification of campylobacter coli isolates from animals and humans by bacterial restriction endonuclease dna analysis.ninety-nine campylobacter coli isolates were examined by bacterial restriction endonuclease dna analysis (brenda) with hindiii. isolates from poultry from the same environment had identical patterns, patterns of isolates carried by suckling piglets were generally the same as those of isolates recovered from their dams, and one human patient yielded the same brenda type when sampled 6 weeks later. the 14 human isolates examined produced 11 distinct brenda types. forty-three c. coli isolates from ...19846093690
toxins produced by campylobacter jejuni and campylobacter coli. 19846141372
analysis of campylobacter jejuni antigens with monoclonal antibodies.to develop monoclonal reagents for antigenic analysis and serotyping of campylobacter spp., hybridoma cell lines were produced by fusion of mouse myeloma cells and spleen cells from mice immunized with formalin-treated campylobacter jejuni organisms. an enzyme immunoassay was used for preliminary screening of the cell culture supernatants and ascites. twenty-nine clones which reacted with the immunogen were obtained. seven of these clones were positive in passive hemagglutination tests with shee ...19846365954
[detection of campylobacter jejuni and campylobacter coli in the routine laboratory]. 19846393616
chemical composition and biological activity of lipopolysaccharides prepared from type strains of campylobacter jejuni and campolybacter coli.lipopolysaccharides (lps) were extracted from seven type strains of campylobacter jejuni and one type strain of campylobacter coli with 45 per cent aqueous phenol. the sugar components present in all lps were glucose, galactose, l-glycero-d-manno-heptose, 2-keto-3-deoxy-octulosonic acid and glucosamine. all but one lps contained galactosamine, and two strains contained in addition mannose. the fatty acids present were 3-hydroxy-tetradecanoic acid, n-hexadecanoic acid and trace amounts of n-tetra ...19846440410
[antimicrobial susceptibility of campylobacter jejuni and campylobacter coli isolated from human and animals in japan]. 19846442333
isoprenoid quinone content and cellular fatty acid composition of campylobacter species.a total of 36 strains of campylobacter species were examined for isoprenoid quinones and cellular fatty acids. the isoprenoid quinone content was determined by reverse-phase high-pressure liquid chromatography, and the fatty acids were determined by capillary gas-liquid chromatography. all campylobacter species contained menaquinone-6 (2-methyl-3-farnesyl-farnesyl-1,4-naphthoquinone) and a methyl-substituted menaquinone-6 (2,[5 or 8]-dimethyl-3-farnesyl-farnesyl-1,4-napthoquinone) as the major i ...19846470096
differentiation of campylobacter species by protein banding patterns in polyacrylamide slab gels.soluble protein extracts of 37 catalase-positive strains of campylobacter species were examined by polyacrylamide slab gel electrophoresis (page). electrophoretic banding patterns showed good correlation with biochemical tests and with available dna homology data in distinguishing species of campylobacter but did not differentiate subspecies or biotypes. page patterns indicated that campylobacter coli is a distinct species. furthermore, the page patterns indicated that c. jejuni and nalidixic ac ...19846490829
new, extended biotyping scheme for campylobacter jejuni, campylobacter coli, and "campylobacter laridis".a biotyping scheme using improved media and methods for the detection of hippurate hydrolysis, rapid h2s production, and dna hydrolysis was applied to 1,826 cultures of campylobacter jejuni, campylobacter coli and "campylobacter laridis" isolates from human and nonhuman sources. four biotypes were identified among c. jejuni: 57.3% of the isolates belonged to biotype i; 36.0%, to biotype ii; 4.0%, to biotype iii; and 2.7%, to biotype iv. c. coli organisms were differentiated into biotype i (67.0% ...19846490850
plasmid content and pathogenicity of campylobacter jejuni and campylobacter coli strains in the pregnant guinea pig model.strains of campylobacter jejuni and c coli from human and animal sources were assessed for pathogenicity by their capability to produce abortions in pregnant guinea pigs. the pathogenicity rate varied from 7% to 87% for the c jejuni strains and from 0% to 53% for the c coli strains. plasmid carriage was not found to correlate with pathogenicity as determined by ability of the strains to produce abortions in the pregnant guinea pig model.19846497123
campylobacter jejuni and campylobacter coli inoculation of neonatal calves.three groups of neonatal calves were inoculated orally with pathogenic strains of campylobacter jejuni or c coli. the calves developed a mild, self-limiting enteritis characterized by thick mucoid feces. bacteremia and fecal shedding of campylobacter were sporadic in all inoculated calves. two groups of calves were killed 1 to 3 weeks after inoculation to study the pathogenesis of infection. postmortem culture of tissues revealed c jejuni or c coli most frequently in the ileum, cecum, colon, and ...19846497139
does the occurrence of plasmids in campylobacter jejuni and campylobacter coli isolates correlate with antibiotic resistance? 19846499842
comparison of antimicrobial susceptibility patterns of campylobacter jejuni and campylobacter coli.to determine whether employing antibiograms is useful to separate campylobacter jejuni and campylobacter coli, we determined the mics of 12 antibiotics for 104 human clinical strains and 74 swine strains. of 74 swine strains, 5 (7%) were hippurate positive, as were 93 (89%) of 104 human strains. the 12 antimicrobial agents tested were ampicillin, amoxicillin, clindamycin, chloramphenicol, erythromycin, furazolidone, norfloxacin, nalidixic acid, rosoxacin, rosaramicin, tetracycline, and sch 32063 ...19846508265
improved biotyping schemes for campylobacter jejuni and campylobacter coli.campylobacter jejuni (20 strains) and campylobacter coli (12 strains) were assigned to four biovars for each species based on phenotypic tests that were easy to perform and interpret. the resulting biotyping schemes offer a greater degree of distinction among c. jejuni and c. coli strains than any of the other biotyping schemes previously described for these organisms.19846511880
a pulmonary abnormality of newborn lambs.nineteen pregnant ewes deliberately infected orally with campylobacter coli/jejuni at either 80 or 120 days gestation and accidentally subjected to a period of heat stress during the latter half of pregnancy produced clinically normal but growth-retarded lambs at term. a group of 7 pregnant ewes uninfected with campylobacter but subjected to the same heat stress produced lambs clinically and morphologically similar to the lambs of the other 2 groups. microscopically, the lungs from 11 lambs show ...19846512027
antibacterial activities of nitrothiazole against campylobacter jejuni and campylobacter coli.niridazole (ambilhar) and three other newly synthesized nitrothiazole derivatives were highly active against 19 microaerophilic campylobacters (minimum concentration required to inhibit 50% of strains [mic50], 0.0075 to 0.015 mg/liter). there were, however, considerable differences in the susceptibility among strains tested, and one nitrothiazole derivative was rather inactive (mic50, 2 mg/liter). nitroimidazole derivatives, such as metronidazole and tinidazole, were less active (mic50, 2 and 4 ...19846517542
chemical studies of partially hydrolysed lipopolysaccharides from four strains of campylobacter jejuni and two strains of campylobacter coli.lipopolysaccharides (lps) from four strains of campylobacter jejuni and two strains of c. coli were partially hydrolysed with 1% acetic acid. subsequent chloroform extraction led to the formation of a polysaccharide-containing aqueous layer, an interfacial material and a lipid a-containing chloroform layer. the polysaccharides contained the neutral sugars, amino sugars, 2-keto-3-deoxy-octonic acid, and part of the phosphorus present in the undegraded lps. the lipid as were made up of glucosamine ...19846527123
detection of campylobacter coli in diarrhoeal and non-diarrhoeal children in india.the role of campylobacter coli as an etiological agent of diarrhea in children and the pathogen's presence in nondiarrheal children was examined in the region of chandigarh, india, from may, 1981, to july, 1982. a total of 768 fecal samples from 447 children, 159 symptomatic and 288 asymptomatic, was collected, along with food, water, soil, and animal dung samples from the village. in diarrheal and nondiarrheal groups, c. coli was isolated with frequencies of 3.1% and 3.8% respectively. this ...19846530531
serotyping and biotyping of campylobacter jejuni and campylobacter coli from sporadic cases and outbreaks in norway.of 172 thermophilic campylobacters isolated from human cases of gastroenteritis in norway, 149 (86.6%) were classified as campylobacter jejuni, whereas 23 isolates (13.4%) belonged to campylobacter coli. c. jejuni biotype 1 comprised 66.3% and c. jejuni biotype 2 comprised 20.3% of the total number. using 50 unabsorbed antisera, we were able to serotype 109 (80.1%) of 136 campylobacters on the basis of heat-stable antigens identified by means of passive hemagglutination. the typable strains fell ...19846699144
campylobacter coli septicaemia associated with septic abortion.a 19-year-old patient was 27 weeks pregnant when admitted to hospital with fever, chills and premature labours. the following day she aborted. campylobacter coli was isolated from blood cultures, maternal placenta and amniotic fluid and from the ear, nose and pharynx of the stillbirth. campylobacter could not be isolated from stool specimens after antimicrobial treatment had been started.19846735483
dna relatedness among strains of campylobacter jejuni and campylobacter coli with divergent serogroup and hippurate reactions.eleven strains of campylobacter from earlier fluorescent-antibody studies were examined by dna hybridization to determine their species. three of the strains hydrolyzed sodium hippurate, and eight did not. four of the hippurate-negative strains were in campylobacter jejuni serogroups, and the remaining strains were in both c. jejuni and campylobacter coli serogroups. dna relatedness to type strains of c. jejuni and c. coli indicated that all three of the hippurate-positive strains and two of the ...19846746886
the serotype and biotype distribution of clinical isolates of campylobacter jejuni and campylobacter coli over a three-year period.two hundred eighty-five isolates of campylobacter jejuni-campylobacter coli from children with gastroenteritis at the hospital for sick children (toronto, canada) over a three-year period were biotyped by the hippurate hydrolysis test and serotyped on the basis of thermostable, soluble antigens by the passive hemagglutination technique. hippurate-negative strains (c. coli) were only 3.2% of the isolates. ninety-seven percent of the isolates were serotypable with 55 antisera. about half of the st ...19836827141
house flies (musca domestica) as possible vectors of campylobacter fetus subsp. jejuni.a total of 161 strains of campylobacter fetus subsp. jejuni were isolated from house flies (musca domestica). the carrier rates detected were 50.7% in flies captured on a chicken farm and 43.2% in flies from a piggery. the relative prevalences of campylobacter coli, c. jejuni, and nalidixic acid-resistant thermophilic campylobacters were 90.1, 6.2, and 3.7%, respectively. the results indicate that flies may play a linking role in the epidemiology of campylobacter infection in humans by transmitt ...19836830213
serotypes of campylobacter jejuni and campylobacter coli in bacteremic, hospitalized children. 19836833802
occurrence of plasmid dna in serologically defined strains of campylobacter jejuni and campylobacter coli.forty campylobacter jejuni and 17 campylobacter coli strains that constitute the set of reference strains for our serotyping scheme were each examined for the presence of plasmid dna. agarose gel electrophoresis of alkaline-extracted dna showed the occurrence of 29 bands in 11 c. jejuni strains and 40 bands in c. coli strains. plasmids ranged in size from 1.6 to 70 megadaltons. most strains that carried plasmids had between 2 and 6 of them; however, one strain had 14 plasmids, and two strains co ...19836840847
identification and characterization of campylobacter jejuni outer membrane proteins.outer membrane proteins from isolates of campylobacter jejuni were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. sarcosinate-insoluble membrane preparations were outer membrane enriched based on increased ketodeoxyoctonate concentrations, the presence of surface-exposed 125i-labeled proteins that were hydrophobic, and similarity to membrane vesicle (bleb) sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles. most isolates contained a single major band with ...19836618667
serotyping of campylobacter jejuni and campylobacter coli on the basis of thermostable antigens.hippurate hydrolysis tests performed on the serotype reference strains of the serotyping scheme based on thermostable antigens under development for campylobacter jejuni showed that 42 strains were campylobacter jejuni and 17 were campylobacter coli. moreover, only four (0.2%) of 2025 hippurate positive campylobacter jejuni isolates reacted in campylobacter coli antisera and 12 (4.3%) of the 282 campylobacter coli reacted in campylobacter jejuni antisera. evidently each species has its own array ...19836628376
campylobacter jejuni and campylobacter coli serotypes isolated from chickens, cattle, and pigs.a total of 191 campylobacter jejuni and 125 campylobacter coli were isolated from the intestinal content of 398 chickens, 421 cattle, and 203 pigs. all 108 chicken isolates and 73 of 80 cattle isolates were c. jejuni, but 115 of the 118 pig isolates were c. coli. a total of 84% of the c. jejuni and 64% of the c. coli isolates were typed on the basis of thermostable antigens with 20 antisera prepared against frequently occurring serotypes in campylobacter enteritis in man (15 c. jejuni, 6 c. coli ...19836630466
swine dysentery: protection against experimental challenge following single dose parenteral immunisation with inactivated treponema hyodysenteriae.groups of five pigs were vaccinated at three to four weeks old with either formolised treponema hyodysenteriae in oil adjuvant alone, formolised t hyodysenteriae in oil adjuvant plus formolised campylobacter coli in oil adjuvant, or sterile medium in oil adjuvant (as a control). each group was challenged four weeks after vaccination by oral dosing on two consecutive occasions with pure cultures of the homologous strain of t hyodysenteriae plus direct contact with two pigs exhibiting severe swine ...19836635345
isolation and characterization of campylobacter jejuni and campylobacter coli from domestic and wild mammals in norway.a total of 1,262 domestic and wild mammals from norway were surveyed for fecal carriage of campylobacter jejuni and campylobacter coli. of the five species of domestic mammals examined, the highest isolation rate was recorded among swine (100.0%), followed by sheep (8.1%) and cows (0.8%). no strains were recovered from horses or goats. among wild mammals, c. jejuni was isolated from 1 of 23 hares, and no isolated were obtained from three species of cervids and three species of rodents. of the 13 ...19836639033
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