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in vitro susceptibilities of campylobacter jejuni and campylobacter coli to azithromycin and erythromycin.mics of azithromycin and erythromycin for 20 campylobacter coli and 20 campylobacter jejuni strains were determined. the results demonstrated that, for campylobacter species, all high-level erythromycin-resistant strains were also resistant to azithromycin and that azithromycin did not exhibit increased potency in comparison with that of erythromycin.19911659309
identification, characterization, and spatial localization of two flagellin species in helicobacter pylori flagella.flagellar filaments were isolated from helicobacter pylori by shearing, and flagellar proteins were further purified by a variety of techniques, including cscl density gradient ultracentrifugation, ph 2.0 acid disassociation-neutral ph reassociation, and differential ultracentrifugation followed by molecular sieving with a sephacryl s-500 column or mono q anion-exchange column, and purified to homogeneity by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transfer to an ...19911704004
comparison of the survival of campylobacter jejuni and campylobacter coli in culturable form in surface water.six campylobacter jejuni and six campylobacter coli strains were isolated from cows and pigs, and their survival in lake water was compared by viable counts. campylobacter jejuni survived longer in culturable form than c. coli in untreated and membrane-filtered water both at 4 and 20 degrees c. this difference in survival time may be a reason why c. jejuni is generally isolated from surface waters more frequently than c. coli. both species survived better in filtered than in untreated water. thi ...19911913357
immunocytochemical identification of helicobacter pylori in formalin-fixed gastric biopsies.h&e and special histochemical stains are used by most laboratories to identify helicobacter pylori (h. pylori) in gastric biopsy specimens. however, background staining can complicate recognition of h. pylori and small numbers of organisms may be overlooked. additionally, histochemical stains do not distinguish h. pylori from other spiral organisms. we investigated two commercially available monoclonal antibodies, one directed against campylobacter coli and c. jejuni (mab002) and the other again ...19911924280
common and variable domains of the flagellin gene, flaa, in campylobacter jejuni.the organization of the flagellin gene locus in campylobacter jejuni strain in1 (lior 7) was determined using the polymerase chain (pcr) reaction and a series of oligonucleotide primers. two tandemly arranged flagellin genes of approximately 1.7 kb were found to be joined by an intervening segment of c.0.2kb, similar to that reported for campylobacter coli. the 5' flagellin gene, flaa, was generated by pcr and both strands sequenced. comparison of the deduced amino acid sequence for c. jejuni fl ...19911956293
significance of flagella in colonization resistance of rabbits immunized with campylobacter spp.cross-protection among different lior and penner serogroups of campylobacter spp. was studied. rabbits were orally immunized by gastric feeding with campylobacter spp., and 27 to 30 days later, they were challenged with matched or unmatched serogroups by the removable intestinal tie adult rabbit diarrhea (ritard) procedure. when immunized animals were challenged with different lior serotypes, no protection against colonization was seen; however, when challenged with homologous lior serogroups, p ...19912050397
use of non-radioactive dna probes for detection of campylobacter jejuni and campylobacter coli in stool specimens.dna probes specific for c. jejuni (pdt1720 containing a 1475 base pair fragment) and for c. jejuni and c. coli (pdt1719 containing a 1845 base pair fragment) were isolated from a bacteriophage lambda gt11 genomic library of c. jejuni, using antiserum prepared against a 46 kda major outer membrane protein of c. jejuni. the two probe-fragments had different restriction maps and were only moderately related by dna hybridization analysis. a non-radioactive labelling kit which consisted of alkaline p ...19902402249
morphology and immunoperoxidase studies of intestinal adenomatosis in the blue fox, alopex lagopus.in a blue fox farm about 300 of 400 weanling pups were affected with diarrhoea and prolapse of the rectum. about 40 pups died in an emaciated state after a few days of disease. eight pups were submitted for examination. pathological findings were restricted to the caecum, proximal colon and rectum, with occasional extension to the ileum. affected areas revealed a thick and rigid intestinal wall, a narrow lumen and a thickened, wrinkled and sometimes ulcerated mucosa. histologically, the mucosa s ...19902195067
chloramphenicol resistance in campylobacter coli: nucleotide sequence, expression, and cloning vector construction.a chloramphenicol-resistance determinant (cmr), originally cloned from campylobacter coli plasmid pnr9589 in japan, was isolated and the nucleotide sequence determined, which contained an open reading frame of 621 bp. the gene product was identified as cm acetyltransferase (cat), which had a putative amino acid sequence that showed 43% to 57% identity with other cat proteins of both gram+ and gram- origin. although expression of the cat gene was constitutive in both c. coli and escherichia coli, ...19902227449
[evaluation of a latex agglutination method for the rapid identification of campylobacter jejuni/coli].a latex agglutination assay was developed to identify campylobacter jejuni and campylobacter coli. we evaluated the specificity, reproducibility and utility of the assay for clinical use and the following results were obtained. 1) to prepare standardized antigen, bacterial cells must be suspended to a density of 1 to 5 mcfarland unit, and heated at 121 degrees c for 10 to 30 min. 2) bacterial cells may be suspended either in the solution provided with the kit, or in physiological saline, without ...19902082036
campylobacter jejuni versus campylobacter coli in developing countries: how accurate are prevalence estimates? 19902373882
purification, characterization, and localization of a protein antigen shared by thermophilic campylobacters.a protein antigen with an apparent molecular weight (mr) of 31,000 was isolated from 0.2 m glycine hydrochloride (ph 2.2) extracts of a typical human fecal isolate, campylobacter jejuni vc74. the protein was purified to homogeneity on a preparative scale by immunoaffinity chromatography followed by molecular sieving with a superose 12 column. isoelectric focusing under nondenaturing conditions indicated a pi of 9.3, and amino acid composition analysis showed that the protein was unusually rich i ...19902380360
natural transformation in campylobacter species.growing cells of campylobacter coli and c. jejuni were naturally transformed by naked dna without the requirement for any special treatment. transformation frequencies for homologous chromosomal dna were approximately 10(-3) transformants per recipient cell in c. coli and 10(-4) in c. jejuni. maximum competence was found in the early log phase of growth. campylobacters preferentially took up their own dna in comparison with escherichia coli chromosomal dna, which was taken up very poorly. three ...19902404960
biotypes, serogroups and antibiotic susceptibility of campylobacter jejuni and campylobacter coli in chile.phenotypic markers were studied in 105 strains of thermophilic campylobacters isolated from human beings, animals and drinking water in santiago, chile. the strains were identified as campylobacter jejuni (n = 49) and campylobacter coli (n = 56). biotypes i and ii (lior schema) accounted for 96% c. jejuni isolates, the other 4% being biotype iv but the two biotypes of c. coli were about equally represented. a total of 28 serogroups (lior's heat-labile antigens) were identified. lior 13, 9, 79, 2 ...19902181024
use of tri-gas incubator for routine culture of campylobacter species from fecal specimens.we evaluated a tri-gas incubator for campylobacter isolation to be used instead of an anaerobic jar. fecal specimens were cultured in duplicate onto charcoal selective medium and incubated at 43 degrees c for 48 h in two different environments: a tri-gas incubator (forma scientific) adjusted to provide an atmosphere of 10% co2, 10% o2, and the balance n2; and evacuated anaerobic jars with a replacement gas mixture of 10% co2, 5% o2, and 85% n2. a total of 106 campylobacter jejuni and 8 campyloba ...19902280012
use of an alkaline phosphatase-labeled synthetic oligonucleotide probe for detection of campylobacter jejuni and campylobacter coli.a commercially available synthetic nucleic acid probe (snap) conjugated to alkaline phosphatase was compared with standard culture techniques for detecting campylobacter species. the snap was able to detect either 5 ng of c. jejuni dna or 10(5) cfu of bacteria. the snap could also detect dna extracted from 10(5) cfu in mock-infected stool samples. the snap detected c. jejuni and c. coli but showed no reactivity with c. laridis, c. fetus subsp. fetus, c. fetus subsp. venerealis, c. fennelliae, "c ...19902380381
emergence of aminoglycoside resistance genes aada and aade in the genus campylobacter.resistance to streptomycin or spectinomycin or both in five campylobacter coli strains, two campylobacter jejuni strains, and a campylobacter-like strain was studied by enzymatic assays and dot blot hybridization. resistance was due to 6- or 3",9-aminoglycoside adenylyltransferases and to new types of phospho- and adenylyltransferases.19902168151
polynucleotide sequence relationships among flagellin genes of campylobacter jejuni and campylobacter coli.dna probes that encode a complete flagellin gene and various internal regions of the campylobacter coli vc167 flagellin genes were hybridized to 30 strains of c. coli or c. jejuni from 20 different lior serogroups. the results indicated a high overall degree of homology among all of the strains examined. although the most variable regions occurred within the middle of the gene, significant dna homology was observed among many serogroups in this region of the molecule.19902370114
immuno-histochemical and -cytochemical evidence suggesting the presence of campylobacter jejuni and campylobacter coli in cases of porcine intestinal adenomatosis.antisera against a number of campylobacter species were used in immuno-histochemical and -cytochemical studies on cases of porcine intestinal adenomatosis. avidin-biotin-complex (abc) and streptavidin immunoperoxidase methods were used on formalin-fixed, paraffin-embedded and frozen sections. protein a gold method was used on formaldehyde fixed and frozen sections for immuno-cytochemistry. the antisera used were raised in rabbits by subcutaneous or intravenous injection of living or formalin tre ...19902099622
electrolyte abnormalities associated with diarrhea in rhesus monkeys: 100 cases (1986-1987).serum electrolyte values from 100 rhesus monkeys with diarrhea were reviewed. the most frequent finding was hyponatremia (88%), with hypochloremia next most frequently detected (80%). metabolic acidosis was less common (59%) and usually associated with high anion gap values. associations between electrolyte abnormalities and age, housing, or case outcome were not found. bacteriologic culturing was performed on fecal specimens from 90 monkeys. campylobacter coli or c jejuni alone was isolated fro ...19902112122
tropical arthritis in papua new guinea: a reactive arthritis.an idiopathic, asymmetrical oligoarthritis affecting young adults is prevalent in the tropical regions of africa, asia and melanesia. a serological study was undertaken in 23 consecutive patients with polyarthritis. each patient was assigned two paired control subjects. a standardized history, physical examination and investigations were performed. acute and convalescent sera were examined for evidence of recent infection. the presence of hla-b27 was determined. twelve (52%) of the 23 patients h ...19902300025
adherence, enterotoxigenicity, invasiveness and serogroups in campylobacter jejuni and campylobacter coli strains from adult humans with acute enterocolitis.two hundred campylobacter jejuni and campylobacter coli strains from the same number of adult swedish patients with acute enterocolitis were tested regarding adherence to and invasiveness in hep-2 cells and for enterotoxigenicity by the cho-cell assay. the serogroup characteristics, heat-stable and heat-labile, for each strain were also investigated. eighty-four percent of the strains were classified as c. jejuni and 16 percent as c. coli. all of the strains were adherent to hep-2 cells, 39% wer ...19902302354
isolation and antimicrobial susceptibility of campylobacter coli and campylobacter jejuni from slaughter hogs.cultural examination of cecal contents from 109 market weight hogs slaughtered in prince edward island during may-july 1988 yielded 62 isolates of campylobacter coli and seven campylobacter jejuni. a commercial latex agglutination test helped to confirm the identification of campylobacter. when tested against four drugs: erythromycin, tetracycline, kanamycin and ampicillin, 11 isolates showed multiple resistance. resistance to erythromycin was seen in 19% and 28.6% of campylobacter coli and camp ...19902308529
genomic organization and expression of campylobacter flagellin genes.campylobacter coli vc167, which undergoes an antigenic flagellar variation, contains two full-length flagellin genes, flaa and flab, that are located adjacent to one another in a tandem orientation and are 91.5% homologous. the gene product of flab, which has an mr of 58,946, has 93% sequence homology to the gene product of flaa, which has an mr of 58,916 (s. m. logan, t. j. trust, and p. guerry, j. bacteriol. 171:3031-3038, 1989). mutational analyses and primer extension experiments indicated t ...19902318805
a cohort study of enteric campylobacter infection in children from birth to two years in bangui (central african republic).a cohort of 111 children from bangui, central african republic, was followed for enteric campylobacter infection from birth until the age of 2 years. stools were examined at each episode of diarrhoea, and bi-weekly up to the age of 6 months irrespective of the presence of diarrhoea. 349 episodes of diarrhoeal illness were recorded (1.6 per child-year). campylobacters were isolated from 41 (11.7%) of the 349 episodes, but in half of them another enteric pathogen was also isolated. campylobacters ...19902345912
in vitro susceptibility of campylobacter jejuni and campylobacter coli isolated in austria to erythromycin and ciprofloxacin.more than 200 strains of campylobacter (c.) jejuni/coli isolated in 1985 and 1987/88 from human fecal specimens were tested for their susceptibility to erythromycin and ciprofloxacin. their mic90 as assessed by agar dilution tests was 2.0 and 0.5 mg/l, respectively. thus, all strains were regarded as susceptible to ciprofloxacin. with 2 out of 55 strains of c. coli the mic of erythromycin was 8.0 mg/l. therefore, only 3.6% of the c. coli strains were resistant to erythromycin. all 209 strains of ...19902360966
evidence of reinfection with multiple strains of campylobacter jejuni and campylobacter coli in macaca nemestrina housed under hyperendemic conditions.a prospective bacteriologic study of 18 infant pig-tailed macaques (macaca nemestrina) housed in a nursery facility in which campylobacter spp. are endemic was undertaken to determine the epidemiology of infection and reinfection. the isolates of campylobacter jejuni and c. coli cultured from 8 of the 18 infants were characterized by serotyping, dna hybridization, and polyacrylamide gel electrophoresis protein profiles. the chronology of infection was indicative of multiple reinfections with dif ...19902365455
evaluation of cytotoxic activity in fecal filtrates from patients with campylobacter jejuni or campylobacter coli enteritis.we sought to determine the prevalence of cytotoxic activity in fecal filtrates from persons with c. jejuni or c. coli enteritis. stool specimens were collected from 20 persons with c. jejuni or c. coli enteritis, 20 persons with acute diarrheal illnesses of other causes, and 9 healthy, asymptomatic persons. fecal filtrates were then incubated with chinese hamster ovary (cho) or hela cells. the fecal filtrate from 1 of the 20 (5%) persons with campylobacter enteritis was cytotoxic for hela cells ...19902227365
isolation of campylobacter coli from a proliferative intestinal lesion in a ferret. 19902094455
[evaluation of a new medium using red-cell lysate for the isolation of campylobacter jejuni and campylobacter coli].a semiprepared medium containing horse globular lysate was evaluated for the isolation of campylobacter under two incubation atmospheres (5% o2 and candle jar). studying 19 known strains of c. jejuni, 151 human and 230 animal fecal samples. they were seeded on conventional medium (mc) and on the new medium (mlg) and incubated at 42 degrees c for 24 and 48 h. campylobacter strains grew better in 5% o2 atmosphere after 48 h of incubation. all the strains that grew or were isolated on mc, could als ...19902287716
comparative in vitro activity of cefpirome and cefepime, two new cephalosporins.in in vitro tests the broad-spectrum cephalosporins cefpirome and cefepime were highly active against enterobacteriaceae, although often less so against strains resistant to amoxicillin-clavulanate and ticarcillin-clavulanate, and against most strains of acinetobacter spp. and aeromonas hydrophila. they were also active against pseudomonas aeruginosa, although strains with non-plasmid mediated beta-lactam resistance were sometimes less sensitive. other pseudomonas spp. varied in their sensitivit ...19902226498
identification of campylobacter jejuni and campylobacter coli from waste water by sds-disc-page of whole cell protein.analysis of the electrophoretic profiles of whole cell proteins of campylobacter strains from waste water was used to differentiate c. jejuni from c. coli. the results show, that biotyping of campylobacters from waste water may results in a false species differentiation. some of the campylobacter strains originally typed as c. coli, were proved to be atypical c. jejuni strains. therefore, in addition to biotyping and serotyping, electrophoretic typing is recommended to ensure correct identificat ...19902080967
infectoepidemilogic and immunologic implications of campylobacter coli enteritis in one newborn monozygotic twin pair.since campylobacter infections in humans at early age have not been thoroughly documented yet, it seemed interesting to report the occurrence of two simultaneous cases of c. coli enteritis in one newborn monozygotic twin pair. their clinical history, epidemiology and microbiological analysis, development of specific serum and mucosal immunity as well as total ig synthesis are discussed on the basis of their close genetic and environmental relationships.19892604587
in vivo antigenic variation of campylobacter flagellin.campylobacter coli vc167 cells producing either antigenic phase 1 (p1) or phase 2 (p2) flagellins (as determined by characteristic protein and dna patterns) were used to infect rabbits by the removable intestinal tie-adult rabbit diarrhea (ritard) procedure. rabbits infected with p2 cells shed predominantly p2 cells throughout the infection; in rabbits infected with p1 cells, a transition of fecal isolates from p1 to p2 was observed.19892744863
identification of campylobacter jejuni and c. coli by gel electrophoresis of the outer membrane proteins.analysis of the electrophoretic profiles of the outer membrane proteins could be used to differentiate campylobacter jejuni (16 strains) from campylobacter coli (10 strains). this observation was confirmed by the study of dna homology obtained by a quantitative filter hybridization method. the hippurate hydrolysis test gave a poor correlation with the results of differentiation obtained by dna homology studies and outer membrane protein profile.19892745680
campylobacter jejuni and campylobacter coli diarrhoea in rural and urban populations in yugoslavia.during a 4-month period during the summer of 1985, campylobacters were isolated from 338 (16.3%) of 2080 patients with acute diarrhoea attending the university hospital of infectious diseases in zagreb. of these isolates 220 (64.1%) were campylobacter jejuni and 118 (34.9%) were c. coli. the patients were drawn from three residential zones in and around zagreb: inner city, peripheral city and rural. incidences of campylobacter diarrhoea ranged from 71 per 100,000 per year in inner city residents ...19892917618
reproduction of proliferative enteritis in gnotobiotic pigs.gnotobiotic pigs dosed orally with filtrates (0.8 and 0.65 micron) of intestinal mucosa from a pig affected by proliferative haemorrhagic enteropathy developed lesions of proliferative enteritis, affecting mainly the ilea. other piglets dosed with filtrates of affected mucosa from the same source and from other proliferative haemorrhagic enteropathy or intestinal adenomatosis mucosae, did not develop lesions. all inocula contained numerous campylobacter-like organisms evident in stained smears, ...19892922502
structural and antigenic properties of campylobacter coli lipopolysaccharides.lipopolysaccharides (lpss) from o-serotype reference strains and from serotyped isolates of campylobacter coli were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in combination with silver staining and immunoblotting to elucidate the molecular basis for the thermostable antigenic diversity in c. coli. electrophoresed low-mr lps was detectable by silver staining and immunoblotting, but high-mr lps was demonstrable only by immunoblotting. eight different antigenic specifici ...19892807533
enterotoxin production and serogroups of campylobacter jejuni and campylobacter coli from patients with diarrhea and from healthy laying hens.enterotoxin production, a possible virulence factor, was determined in campylobacter jejuni and campylobacter coli by two different techniques, the cho cell test and the gm1 enzyme-linked immunosorbent assay. the frequency of enterotoxigenic campylobacter strains was 32% in strains from both humans with acute enteritis and healthy laying hens, as measured by the cho cell test. the cho cell test was significantly more sensitive than the gm1 enzyme-linked immunosorbent assay in the detection of en ...19892754001
[isolation of campylobacter jejuni and campylobacter coli from children with acute intestinal diseases].in this work the data obtained in the examination of 338 children with diarrhea, aged 5 days to 14 years, are presented. the methods used for the collection of samples and their storage till the moment of inoculation are described. the possibility of using the microscopic examination of campylobacter-containing native feces is shown. the work resulted in the isolation of 85 c. jejuni and c. coli strains. as shown in this work, the isolation of campylobacter depended on the age of children and th ...19892652954
campylobacter infection in humans.campylobacter jejuni and campylobacter coli are now recognized as common bacterial cause of enteritis in humans. they infect the healthiest of persons resulting in a self-limiting diarrhoea often accompanied by mucus and blood in the stool. on the other hand c. fetus subsp fetus attacks otherwise debilitated persons whose body defences have been weakened by underlying conditions, and causes extragastrointestinal campylobacteriosis. c. pylori, which is able to survive in the stomach acid environm ...19892693070
evidence for posttranslational modification and gene duplication of campylobacter flagellin.a gene encoding a flagellin protein of campylobacter coli vc167 has been cloned and sequenced. the gene was identified in a pbr322 library by hybridization to a synthetic oligonucleotide probe corresponding to amino acids 4 to 9 of the n-terminal sequence obtained by direct chemical analysis (s. m. logan, l. a. harris, and t. j. trust, j. bacteriol. 169:5072-5077, 1987). the dna was sequenced and shown to contain an open reading frame encoding a protein with a molecular weight of 58,945 and a le ...19892722741
diversity of serotypes of campylobacter jejuni and campylobacter coli isolated in laboratory animals.one hundred nineteen isolates of campylobacter jejuni and campylobacter coli from nine laboratory animal species were serotyped using antisera to 20 penner serotypes commonly isolated from cases of human enteric infections. although c. jejuni and c. coli were isolated from laboratory animals with diarrhea, the majority were cultured from asymptomatic animals (81%). seven of twenty-two isolates from animals with diarrhea were serotype 4 (32%) and three were serotype 1 (14%). sixty-one of the 119 ...19892724920
studies on the chemical constitution of cell wall lipo-oligosaccharide from campylobacter coli labet 227.the lipo-oligosaccharide (los) from campylobacter coli labet 227 was extracted by aqueous phenol. after delipidation and gel chromatography, two oligosaccharides were isolated. the higher molecular weight material os (i) which was estimated to contain six to seven sugar units was found to contain glucose, galactose, 2-acetamido-2-deoxyglucose, 2-acetamido-2-deoxygalactose and heptose. analysis of the partially methylated alditol acetates by g.c.-m.s. revealed the presence of terminal hexoses, a ...19892489074
coumamidines, new broad spectrum antibiotics of the cinodine type. iii. microbiologic activity of coumamidine gamma 1.the coumamidines are novel antibiotics with activity against a wide spectrum of aerobic gram-positive and gram-negative bacteria. all microbiological studies were performed on coumamidine gamma 1. the mic90s (micrograms/ml) of coumamidine are as follows: staphylococcus aureus 1.0, streptococcus pyogenes 8, enterobacteriaceae 2.0, pseudomonas aeruginosa 8, campylobacter jejuni and campylobacter coli 1, legionella pneumophila 8, haemophilus influenzae 0.5, neisseria gonorrhoeae 0.5. coumamidine ha ...19892498268
[in vitro susceptibility of campylobacter jejuni and campylobacter coli to quinolone antibiotics].in this study, in vitro susceptibilities of 24 campylobacter strains isolated from fecal samples were tested for quinolone antibiotics. 13 of the strains were identified as c. jejuni and 11 of them were c. coli. antibiotics which were included in this study were ciprofloxacin, ofloxacin, pefloxacin and fleroxacin. antibiotic susceptibility tests were performed on mueller-hinton agar (supplemented with 5% sheep blood) using the agar dilution method. results showed that quinolone antibiotics are v ...19892518646
characterization and antibiotic sensitivity of campylobacter jejuni and campylobacter coli isolated from children at the lagos university teaching hospital (luth), lagos, nigeria.one hundred and thirteen rectal swabs and 87 faecal samples obtained from the paediatric casualty of the lagos university teaching hospital, lagos, nigeria were processed for campylobacter jejuni/coli. twenty-two (11%) of the specimens were positive for c. jejuni. biotyping results showed that nine (41%) and eight (36%) belonged to c. jejuni biotypes i and ii respectively, while three (14%) and two (9%) belonged to c. coli biotypes i and ii. serotyping results revealed that c. jejuni biotypes i ...19892651685
characterization of the porins of campylobacter jejuni and campylobacter coli and implications for antibiotic susceptibility.the major outer membrane protein was extracted from campylobacter coli by triton x-100/edta fractionation of cell envelopes. this heat-modifiable protein was shown to have pore-forming activity in black lipid bilayers. the c. coli porin formed a relatively small cation-selective pore with a mean single-channel conductance of 0.53 +/- 0.16 ns in 1.0 m kcl. there was no evidence of oligomer formation, which suggested that each protein monomer formed a pore. pore-forming activity of the c. coli por ...19892543277
oligonucleotide probe for detection and identification of campylobacter pylori.we have developed a novel and practical dna-rna hybridization assay for the detection and identification of campylobacter pylori in the gastric mucosa. this technique utilizes a [32p]ddatp-labeled synthetic oligonucleotide probe complementary to a nucleotide sequence present in c. pylori 16s rrna. this probe is very sensitive and reacted with all 23 strains of c. pylori tested. it is also highly specific, since there was no cross-reactivity with the heterologous organisms campylobacter coli, c. ...19892480360
interaction of campylobacter species with antibody, complement and phagocytes.the opsonisation of four different campylobacter species for human neutrophils was studied using a chemiluminescence system and electron microscopy. opsonisation of campylobacter fetus, campylobacter coli, and campylobacter jejuni was mediated by antibody and enhanced by complement. antibody was not, however, required for the phagocytosis of campylobacter pylori because it activates the classical pathway of complement directly. this unusual property may be important in the pathogenesis of c pylo ...19892788111
in vitro activities of lomefloxacin and temafloxacin against pathogens causing diarrhea.the in vitro activities of temafloxacin (a63004) and lomefloxacin (sc-47111; ny-198) were compared with those of seven other antibiotics against 146 isolates of bacterial enteric pathogens, including campylobacter jejuni and campylobacter coli. ciprofloxacin was the most active drug against the salmonella, shigella, yersinia, and vibrio spp. tested. lomefloxacin, temafloxacin, and difloxacin were the most active drugs tested against campylobacter spp. (mic for 90% of strains, 0.125 to 0.25 micro ...19892802563
comparative in-vitro activity of meropenem on clinical isolates from the united kingdom.mics of meropenem were determined for a wide range of common bacteria of clinical importance. for enterobacteriaceae, aeromonas spp., haemophilus influenzae, branhamella catarrhalis, neisseria gonorrhoeae, gardnerella vaginalis, campylobacter coli/jejuni, beta-haemolytic streptococci and anaerobes other than clostridium difficile, mics were almost always within the range 0.002-0.5 mg/l. the activity of meropenem for these organisms was always greater than that of imipenem and piperacillin, and w ...19892808214
a one-year study on thermophilic campylobacters isolated from fecal specimens.during a 12-month period, a systematic screening for thermophilic campylobacters was done on 5723 stool samples of hospitalized patients and out-patients who required stool culture tests for gastrointestinal disturbances. campylobacter strains were isolated in 130 samples (2.27%) and campylobacter jejuni (mainly biotype ii) was the species most frequently isolated. no pronounced seasonal pattern was evident and the age at which infection was more often observed was that from 6 to 24 months. eryt ...19892674635
a study of campylobacter jejuni and campylobacter coli in a river system.campylobacter isolates were detected in 82.1% of river water samples moderately polluted with organic wastes. in the majority of the samples less than 10 campylobacter cfu/100 ml were detectable. the presence of waterfowl and the faecal contamination from a poultry-farm resulted in considerable higher campylobacter contents (greater than 240/100 ml). correlation studies showed a high correlation coefficient (p less than 0.05) between the number of campylobacters and that of total coliforms. camp ...19892684204
population genetics of human and animal enteric campylobacter strains.a total of 125 strains of campylobacter jejuni and campylobacter coli from human and animal hosts were analyzed by multilocus enzyme electrophoresis. ten genetic loci were characterized by the electrophoretic mobilities of their products, and all were found to be polymorphic. the nine loci considered for genetic analysis had an average allele number of 6.9 and a mean genetic diversity of 0.634. the strains were grouped into 64 electrophoretic types (ets) forming two principal clusters; the first ...19892707852
[antibiotic sensitivity of campylobacter jejuni and campylobacter coli strains with special reference to sensitivity to erythromycin and neomycin].166 strains of c. jejuni and 46 strains of c. coli were typed against 14 antibiotics using the agar diffusion test. all strains were resistant against penicillin g, oxacillin and rifampicin. susceptibility were found against chloramphenicol, erythromycin, neomycin, oxytetracyclin, streptomycin and gentamycin. the minimal inhibition concentration was performed for erythromycin and neomycin. all strains tested were inhibited by less than or equal to 2 micrograms/ml neomycin, one strain was resista ...19892710556
serological analysis of the heat-stable antigens involved in serotyping campylobacter jejuni and campylobacter coli.analysis with serotyping antisera showed that carbohydrate determinants were the dominant heat-stable antigens of campylobacter jenuni/coli involved, whereas proteins did not contribute to the serological reactions. lipopolysaccharide (lps) along with a polysaccharide extract from whole bacteria (ps(wb] conferred strain serospecificity. in general, analysis with monoclonal antibodies in passive haemagglutination and co-agglutination tests showed the existence of similar antigenic determinants in ...19892713136
immune response to campylobacter jejuni and campylobacter coli in a cohort of children from birth to 2 years of age.a cohort of 111 children from bangui, central african republic, was surveyed for enteric campylobacter infections from birth to the age of 2 years; stools were examined biweekly in these children until 6 months of age and at least four times per year thereafter until 2 years of age and after each diarrheal episode. blood samples were obtained at birth and at 3, 6, 9, 12, 18, and 24 months of age. antibodies against glycine-extracted membrane antigens, purified flagella, and cholera toxin (ct) we ...19892744860
genomic rearrangements associated with antigenic variation in campylobacter coli.campylobacter coli and campylobacter jejuni share a limited number of highly conserved dna sequences with members of the family enterobacteriaceae. one of these sequences was cloned from c. coli vc167, and the region of homology to the enteric sequences was determined to be confined to a 700-base-pair region. the dna represented in this clone undergoes a programmed, reversible rearrangement in vc167 that is associated with flagellar antigenic variation.19882826396
gene disruption and replacement as a feasible approach for mutagenesis of campylobacter jejuni.campylobacter jejuni and campylobacter coli are important causes of human enteric infections. several determinants of pathogenicity have been proposed based on the clinical features of diarrheal disease and on the phenotypic properties of campylobacter strains. to facilitate an understanding of the genetic determinants of campylobacter virulence, we have developed a method for constructing c. jejuni mutants by shuttle mutagenesis. in the example described here, a kanamycin resistance gene was in ...19882832375
nucleotide sequence analysis of tetracycline resistance gene teto from streptococcus mutans dl5.streptococcus mutans dl5, isolated from the dental plaque of a pig, was resistant to high levels of streptomycin (sm, 20 mg/ml), erythromycin (em, 1 mg/ml), and tetracycline (tc, greater than 100 micrograms/ml), but contained no detectable plasmid dna. the smr and emr determinants were cloned from cellular dna on the self-replicating 5-kilobase-pair (kbp) ecori fragment of pam beta 1 and the 4.2-kbp cryptic plasmid pva380-1, respectively, by transformation of streptococcus sanguis challis. helpe ...19882841293
comparison of methods used to separate the inner and outer membranes of cell envelopes of campylobacter spp.the outer membrane of campylobacter coli, c. jejuni and c. fetus cell envelopes appeared as three fractions after sucrose gradient centrifugation. each outer membrane fraction was contaminated with succinate dehydrogenase activity from the cytoplasmic membrane fraction. similarly the inner membrane fraction was contaminated with 2-ketodeoxyoctonate and outer membrane proteins including the porin(s). the separation of these two membranes was not facilitated by variations in lysozyme treatment, ce ...19882474628
the response of campylobacter jejuni and campylobacter coli in the sydney rock oyster (crassostrea commercialis), during depuration and storage.sydney rock oysters, when allowed to feed in waters containing approximately 10(4) cfu of campylobacter cells per ml, concentrated between 10(2) and 10(3) cfu of the organism per g of oyster tissue, within 1 h. when these contaminated oysters were subjected to depuration, they were effectively cleaned in 48 h. the survival of campylobacter jejuni and campylobacter coli was also investigated. oysters contaminated by feeding and injection were processed as half shells and bottled oysters and were ...19883275320
adherence of campylobacter jejuni and campylobacter coli to porcine intestinal brush border membranes.the adhesion of campylobacter jejuni and c. coli to isolated porcine intestinal brush border membranes was studied by phase-contrast and electron microscopy. approximately 45% of the cell population adhered to the brush borders, possibly in a specific manner. pretreatment of the brush borders with trypsin or pronase, and competitive inhibition with l-rhamnose caused a slight reduction of the adhesion. different forms of pretreatment of the bacterial cells reduced their ability to adhere, but als ...19883046640
characterization of campylobacter jejuni and campylobacter coli isolated from waste water.the raw sewage samples of the investigated municipal sewage plant (sewage flow of about 30,000 m3/d) contained about 10(3) campylobacters/100 ml. the sewage plant reduced 80.8% of organic wastes (cod), 85.0% of colony counts (20 degrees c), 92.5% of total coliforms and 88.0% of campylobacters. the sewage plant effluent contained an average of 1.3 x 10(2) campylobacters/100 ml. about 10(10) campylobacters are discharged with the effluent of the high-output sewage plant into the receiving water ev ...19883057769
genetic characterization of kanamycin resistance in campylobacter coli.the restriction map of plasmid pip1433 from campylobacter coli bm2509, isolated in france, was constructed and the location of the kanamycin (km) and tetracycline (tc) resistance markers determined using probes for apha-3 and teto genes, respectively. these dna probes were used to study a second km- and tc-resistant strain c. coli ua696, isolated in canada. in this strain, the km resistance determinant was located in the chromosome. like pip1433, the km resistance determinant in c. coli ua696 sp ...19882855308
[quantitative and qualitative studies of campylobacter in the sewage of a large city].in a one-year-study wastewater from the sewage system of a big town was examined for the occurrence and distribution of campylobacter spp. the sewage consisted mainly of domestic and industrial wastewater, but also to a small degree of agricultural wastewater and abattoir effluent. preston broth and agar were used as culture media. the number of campylobacter was determined parallel at 37 degrees c and 42 degrees c by the mpn-method. three colonies of each agar plate were isolated and biotyped. ...19883131983
[quantitative and qualitative studies of campylobacter in a sewage treatment plant].the occurrence and distribution of campylobacter spp. in the wastewater of a sewage treatment plant was examined from july 1985 to july 1986. the number of campylobacter was determined in a double set-up by the mpn-method. preston broth and -agar containing fbp supplement were used as nutrient media. the samples were incubated at 37 degrees c and 42 degrees c, respectively. subsequently 1345 isolated campylobacter strains were biotyped. the number of fecal and total coliforms, ph-value and tempe ...19883131984
the relationship between intestinal campylobacter species isolated from animals and humans as determined by brenda.intestinal thermophilic campylobacter species produce stable patterns when subjected to bacterial restriction endonuclease dna analysis (brenda); this technique is therefore of considerable value in epidemiological studies. brenda was used to examine thermophilic campylobacter species from humans, wild and domestic animals. one hundred and ninety-four (61%) of 316 isolates of campylobacter jejuni from humans had brenda patterns which could be matched to those of animal isolates. poultry appear t ...19882837404
[survey for the contamination of campylobacter jejuni and campylobacter coli among raw meat and the environment of poultry processing plants]. 19883137280
a specific dna probe for the identification of campylobacter jejuni.a 6.1 kb dna probe for the human enteric pathogen campylobacter jejuni has been isolated from a genomic library constructed in the plasmid vector pbr322 in escherichia coli. the dna sequence used as a probe was identified from recombinant plasmids following immunological screening of transformants using polyclonal antisera to whole cells and to membrane antigens of c. jejuni. restriction endonuclease fragment mapping of c. jejuni dna inserts from three of the recombinant plasmids showed an overl ...19883171548
the in-vitro activity of pd127,391, a new quinolone.mics of pd127,391 a new 4-quinolone, and of ci934 and ciprofloxacin, two previously reported 4-quinolones, were determined for common clinical bacterial isolates by an agar-dilution method. pd127,391 was the most active drug against enterobacteriaceae and acinetobacter spp (mics less than 0.12 mg/l) and as active as ciprofloxacin against aeromonas spp. (mics less than 0.008 mg/l) and pseudomonas aeruginosa (mics less than 1 mg/l). it was more active than ciprofloxacin against pseudomonas spp. in ...19883182416
infection with campylobacter coli and campylobacter fetus in a patient on continuous ambulatory peritoneal dialysis. 19883183412
detection of two different kanamycin resistance genes in naturally occurring isolates of campylobacter jejuni and campylobacter coli.a total of 225 isolates of campylobacter jejuni and 54 isolates of campylobacter coli were screened for resistance to kanamycin. among these, five resistant isolates of c. jejuni and six resistant isolates of c. coli, all with different plasmid patterns, were identified. each contained at least one plasmid greater than or equal to 41 kilobases in size. the mic of kanamycin for all 11 strains was determined to be greater than or equal to 256 micrograms/ml by an agar dilution method. in addition, ...19883190204
characterization of an unclassified microaerophilic bacterium associated with gastroenteritis.four isolates of an unclassified microaerophilic bacterium resembling campylobacter species were characterized by growth requirements, microscopic examination, biochemical characteristics, antimicrobial susceptibility tests, and protein profile analysis. the unclassified isolates were differentiated from campylobacter jejuni, campylobacter coli, campylobacter fetus subsp. fetus, campylobacter laridis, campylobacter pylori, and an ovine isolate. the bacterium was fusiform shaped with a corrugated ...19883343302
serovars and biovars of campylobacter strains isolated from humans and slaughterhouse animals in northern germany.a total of 318 campylobacter strains from sporadic cases of human enteritis (109 strains) and healthy slaughterhouse animals in northern germany (209 strains) were bio- and serotyped according to the lior typing schemes. three hundred strains were typable (94.3%) and 38 serovars were identified. among human strains 28 serovars were identified with 30% of them belonging to serovar 4. strains from pigs were associated with 25 serovars, the most frequent being serovar 20 (21.2%). fourteen serovars ...19883372398
antimicrobial susceptibility of campylobacter jejuni, campylobacter coli, and campylobacter laridis from cats, dogs, pigs, and seagulls. 19883210481
prevalence of campylobacter in infant, juvenile and adult laboratory primates.a cross-sectional study of campylobacter spp. infection was conducted on 125 infant (macaca nemestrina and macaca fascicularis) in an infant primate nursery housing infants from birth to 18 months of age, and 145 m. nemestrina aged from 4 months to 15 years at another facility (primate field station) housing animals from birth to aged adults. the objective was to determine the prevalence of campylobacter spp. in various age groups and to investigate the correlation with diarrhea. in the infant p ...19883265462
[campylobacter jejuni and campylobacter coli: epidemiologic markers of isolated strains in santiago, chile]. 19883267894
ovine abortion associated with campylobacter coli. 19883354164
characterization of freshly isolated campylobacter coli strains and suitability of selective media for their growth.typical and atypical campylobacter strains were isolated from the colons of cattle and swine by techniques that enabled the selective pressures of antibiotics to be avoided. some cephalothin-susceptible strains and a strain with an indeterminate hippurate reaction were classified as campylobacter coli by dna homology testing. tetracycline-resistant isolates were obtained from animals with no recorded exposure to antibiotics. a selection of 12 c. coli and 6 c. jejuni strains was used to determine ...19883356790
[animal experiments with campylobacter coli using suckling pigs]. 19883395182
antibody response to campylobacter coli in children during intestinal infection and carriage.in the central african republic, etiological studies of diarrhea have shown that campylobacter coli accounts for almost 40% of campylobacter enteric isolations. this prompted us to investigate the antibody response to c. coli infection in children. as expected from the literature on campylobacter jejuni infections, our results show that both infection and carriage elicited antibodies against glycine-extracted membrane antigens, flagella, and cholera toxin. the human antibody response to c. coli ...19883410956
reassessment of selective agars and filtration techniques for isolation of campylobacter species from faeces.four different studies were conducted in order to re-evaluate conventional methods and assess the efficacy of new selective agars and a filtration method for the isolation of campylobacters. skirrow's medium, preston agar, modified ccd agar and fennell's medium, incubated microaerobically at 37 degrees c for 48 h, gave similar campylobacter isolation rates from 225 faecal samples, but the latter two media were more selective. evaluation of modified ccd agar demonstrated that campylobacters could ...19883134202
demonstration of a flagellar antigen shared by a diverse group of spiral-shaped bacteria that colonize intestinal mucus.western blot analysis showed that there is little immunological cross-reactivity of the human pathogens campylobacter jejuni and campylobacter coli with campylobacter pyloridis, except for a very strong cross-reaction between the flagellins. this same antigenic cross-reaction was found with two isolates of gram-negative spiral microaerophilic bacteria that are known to colonize the intestinal mucosa of rodents, but not with the flagellins of a number of other motile bacteria. it is proposed that ...19873818099
utilization of monoclonal antibodies to evaluate the involvement of campylobacter jejuni in proliferative ileitis in syrian hamsters (mesocricetis auratus).the role of campylobacter jejuni in the pathogenesis of proliferative ileitis in syrian hamsters was evaluated with monoclonal antibodies of different specificities. monoclonal antibodies were produced with two different specificities: one for all members of the genus campylobacter tested (antibody 8322-2e6) and one for c. jejuni and campylobacter coli (antibodies 841-2a11, 841-4c6, and 841-5b1). heal sections from healthy hamsters, from hamsters with naturally occurring proliferative ileitis, a ...19873623701
in vitro susceptibility of campylobacter jejuni and campylobacter coli isolated in denmark to fourteen antimicrobial agents.the in vitro susceptibility of 95 recent danish human clinical isolates of campylobacter jejuni and campylobacter coli to 14 antimicrobial agents was determined by an agar dilution technique. c. jejuni and c. coli were considered together. doxycycline, ciprofloxacin, gentamicin, clindamycin, nitrofurantoin and erythromycin were the most active drugs. 2% of the strains tested were resistant to erythromycin (mic greater than 3.1 micrograms/ml). therefore susceptibility testing is suggested if anti ...19873630712
dna probes for identification of tetracycline resistance genes in campylobacter species isolated from swine and cattle.tetracycline-resistant strains of campylobacter jejuni and campylobacter coli from swine and cattle colons were isolated and characterized by hybridization with dna probes. a probe consisting of the 1.8-kilobase (kb) hincii fragment from pua466 was highly specific for the detection of tetracycline resistance (tcr) in c. jejuni and c. coli. the 5-kb tetm dna probe from streptococcus agalactiae plasmid pji3 which has homology with the 1.8-kb hincii fragment from pua466 could also be used to detect ...19873435114
susceptibility of campylobacter species to metronidazole, its bioactive metabolites and tinidazole.the susceptibilities of 115 campylobacters, belonging to five species, to metronidazole, its principal oxidative metabolites and tinidazole were determined by an agar dilution method. the activities of metronidazole and tinidazole were comparable. the hydroxy metabolite was more active than the parent drug against most species, particularly campylobacter jejuni and campylobacter pyloridis. although the acid derivative was less active against c. jejuni and campylobacter coli, it demonstrated an e ...19873436679
serogroups and biotypes of human strains of campylobacter jejuni and campylobacter coli isolated in france.the lior schemes were used for biotyping and serogrouping campylobacter strains isolated during a three year period in bordeaux, france. of the 226 strains tested, 176 were campylobacter jejuni and 50 campylobacter coli. campylobacter jejuni biotype i was the most prevalent (48.2%). biotypes iii and iv of campylobacter jejuni were rare (3.9% and 1.3% respectively). serogroup 4 (17.7%) was the most common serogroup followed by serogroups 46 (11.0%), 29 (10.1%), 9 and 36 (7.9%). eight serogroups c ...19873440456
high frequency of isolation of campylobacter coli from poultry meat in france. 19873440462
[the survival of campylobacter coli in raw milk].the survival of three strains of campylobacter coli (two isolated from milk of a cow with mastitis, one from poultry intestine) was studied in unpasteurized milk. survival of c. coli strains at 37 degrees c varied greatly: bovine strains showed a 1-log10 decrease in viable cells, and the poultry strain showed 5-log10 decrease after 48 hours. bovine strains lived longer (5 days) than poultry strain (2 days) at 37 degrees c. there was no difference between the strains from different origin in unpa ...19873503963
erythromycin-resistant campylobacter infections in thailand.erythromycin therapy was compared with no treatment in a prospective trial of acute diarrheal disease among 100 infants in an orphanage in bangkok. within 24 h of the onset of diarrhea, 50 children received erythromycin ethylsuccinate (40 mg/kg per day) in four divided doses for 5 days. campylobacter jejuni isolated from 31, campylobacter coli isolated from 21, and shigella spp. isolated from 21 of 100 children were the most commonly recognized pathogens; use of a sensitive, nonselective method ...19873579261
seasonal occurrence of campylobacter spp. in surface waters and their correlation with standard indicator bacteria.campylobacter jejuni, campylobacter coli, and a campylobacter-like organism were isolated from a number of natural water sources in central washington, including ponds, lakes, and small mountain streams at elevations ranging from 1,460 to 5,400 feet (ca. 445 to 1,646 m) above sea level. at the two sites where extensive sampling was done, the bacteria were recovered throughout the year. generally, the recovery rates were highest in the fall and winter months and lowest during the spring and summe ...19873579268
campylobacter jejuni/campylobacter coli-associated guillain-barré syndrome. immunoblot confirmation of the serological response.immunoblot (western blot) examination of the sera of 45 patients with the guillain-barré syndrome demonstrated that between three and five immunoreactive bands that were characteristic of a recent campylobacter jejuni/campylobacter coli infection were present in 22 of these patients. the immunoblots paralleled the serological response that is found in campylobacter enteritis and confirmed the specificity of our previous enzyme-linked immunosorbent assays (elisas), which indicates that antecedent ...19873626926
[the epidemiology of campylobacter jejuni and campylobacter coli in the fattening chicken]. 19873589638
atypical campylobacters associated with gastroenteritis.nine strains of campylobacter species other than campylobacter jejuni, campylobacter coli, and campylobacter laridis were isolated from patients with acute diarrhea. all nine strains showed preferred growth at 37 degrees c under microaerophilic conditions. conventional microbiological tests and dna-dna dot blotting were used to identify these strains. three of the nine campylobacter strains hydrolyzed hippurate, reduced nitrate, produced catalase, were resistant to cephalothin, and were shown to ...19873611317
effect of clavulanic acid on susceptibility of campylobacter jejuni and campylobacter coli to eight beta-lactam antibiotics.the effect of clavulanic acid on the susceptibility of 32 strains of campylobacter jejuni and campylobacter coli to eight beta-lactam agents was studied. almost all strains tested became susceptible to amoxicillin and ticarcillin with 1 microgram of clavulanic acid per ml. this compound had little or no effect on susceptibility to penicillin g, cephalothin, cefamandole, and cefoxitin. clavulanic acid had a marginal effect on cefotaxime and moxalactam susceptibility.19873619428
naturally occurring auxotrophs of campylobacter jejuni and campylobacter coli.the nutritional requirements for 439 campylobacter jejuni isolates and 46 campylobacter coli isolates were determined by using a previously described chemically defined medium, campylobacter defined medium. with this medium, 45% of both human and nonhuman c. jejuni isolates demonstrated auxotrophic requirements. none of the 46 c. coli isolates studied demonstrated requirements for amino acids on campylobacter defined medium. the most common auxotrophic requirement among c. jejuni isolates was fo ...19873654939
spontaneous bacterial peritonitis due to campylobacter coli.a patient with alcoholic liver disease and ascites who developed fatal spontaneous bacterial peritonitis due to campylobacter coli is reported herein. this is the first case report of spontaneous bacterial peritonitis due to this organism. campylobacter infections and their relationship to spontaneous bacterial peritonitis are reviewed.19873569775
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