Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| genetic organization of a small cryptic plasmid of helicobacter pylori. | a 2.9-kb cryptic plasmid of helicobacter pylori (hp), phel1, was isolated and the complete nucleotide (nt) sequence was determined. an open reading frame (orf1) was identified encoding a putative polypeptide of 63,709 da, the existence and correct size of which was confirmed by t7 promoter expression analysis. the orf1 sequence showed strong amino-acid sequence identity to a recently identified putative orf1 protein of a cryptic hp plasmid, phpm180, and significant homologies to putative rep pro ... | 1995 | 7489910 |
| cross-reactive antigens shared by pseudomonas aeruginosa, helicobacter pylori, campylobacter jejuni, and haemophilus influenzae may cause false-positive titers of antibody to h. pylori. | cystic fibrosis (cf) patients suffer from many of the gastrointestinal conditions which occur in non-cf individuals, e.g., dyspepsia and peptic ulceration. these symptoms may be caused by helicobacter pylori but could also be due to either pancreatic insufficiency or the intensive antibiotic treatment used in cf patients. since cf patients chronically infected with pseudomonas aeruginosa produce antibodies against a wide range of antigens, including antigens common to many other bacteria, e.g., ... | 1995 | 7697522 |
| incidence and transmission of antibiotic resistance in campylobacter jejuni and campylobacter coli. | one-hundred and two campylobacter clinical isolates were characterized for their in-vitro resistance to erythromycin (1.9%), three fluoroquinolones (31.3-34.3%), tetracycline (43.1%), kanamycin (4.8%), ampicillin (18.6%) and other 16 antimicrobial agents. conjugative transfer of tetracycline and kanamycin resistances among these strains was achieved and small plasmids of 4.3, 4 and 1.9 kb were observed in kanamycin-resistant campylobacter coli strains. | 1995 | 7768766 |
| investigation of helicobacter pylori ascorbic acid oxidating activity. | helicobacter pylori sonicate was shown to oxidize ascorbic acid. ascorbic acid oxidation was determined by chromatography combined with electrochemical detection. water soluble ascorbic acid oxidase activity was rather independent of ph with a ph optimum around 2. by gel filtration the oxidizing activity co-eluted with an absorbency peak at 408 nm. the relative molecular mass (mr) was approximately 14,000. it is suggested that this oxidating activity was caused by a cytochrome c-like molecule. a ... | 1995 | 7773246 |
| restriction fragment length polymorphisms among the flagellar genes of the lior heat-labile serogroup reference strains and field strains of campylobacter jejuni and c. coli. | several typing systems have been described for campylobacter jejuni and c. coli, to assess the complex epidemiology of these important enteric pathogens. in the present study two typing methods, slide agglutination according to the lior scheme, and the demonstration of restriction-fragment length polymorphisms (rflp) of flagellar genes, have been used in parallel on a set of 194 strains. this set comprised 118 sero-reference strains of c. jejuni and c. coli of the lior scheme, as well as 76 clin ... | 1995 | 7781730 |
| identification of campylobacter jejuni on the basis of a species-specific gene that encodes a membrane protein. | to facilitate discrimination between the closely related enteropathogens campylobacter jejuni and c. coli, unique differences in antigenic surface structure were examined. a genomic library of c. jejuni 81116 was constructed in plasmid pbluescriptiisk- and expressed in escherichia coli k-12. rabbit hyperimmune serum raised against c. jejuni atcc 29428 recognized a clone expressing a c. jejuni 24-kda membrane-associated protein. antiserum raised against sonicated recombinant e. coli expressing th ... | 1995 | 7790451 |
| urinary tract infection caused by quinolone-resistant campylobacter coli. | 1994 | 7813508 | |
| identification of campylobacter jejuni, campylobacter coli and campylobacter lari by the nucleic acid amplification system nasbar. | nasbar, an isothermal amplification technique for nucleic acids, was evaluated for the specific identification of campylobacter jejuni, camp. coli and camp. lari. a set of primers and a probe were chosen from the 16s rrna sequence of campylobacter. the probe was hybridized in solution with the amplified nucleic acids of 12 campylobacter species and nine other gram-negative bacteria. the probe was shown to hybridize specifically to the amplified single-stranded rna of camp. jejuni, camp. coli and ... | 1994 | 7822229 |
| occurrence of plasmids and tetracycline resistance among campylobacter jejuni and campylobacter coli isolated from whole market chickens and clinical samples. | twenty whole market chickens, purchased from 10 different stores in the taipei metropolitan area, were examined for the presence of campylobacter jejuni and campylobacter coli. the microorganisms were recovered from 95% of the chickens. a survey of different sites on--breast, thigh and tail--showed that contamination was equally common on all these sites. one hundred and sixty-seven chicken isolates and the 41 clinical isolates of campylobacter jejuni were examined for the occurrence of plasmid ... | 1994 | 7703010 |
| [diarrheal diseases in 1 to 4 week old suckling piglets from problem herds: microbial spectrum, histology, enzyme histochemistry]. | postmortem investigation was performed on 54 scouring (soon after onset of diarrhea) and 23 healthy suckling piglets. the animals were 1 to 4 weeks of age and were collected from 27 herds. in 21 herds diarrhea had been observed for a long time in piglets in this age group. coccidia were found in 34 piglets, rotavirus in 2, cryptosporidia in 2, enterotoxigenic e. coli in 6, attaching and effacing e. coli in 1, cl. perfringens type c in 1, campylobacter coli in 53 and c. hyointestinalis in 8 pigle ... | 1994 | 7709191 |
| bacteraemia caused by campylobacter spp. | the genus campylobacter has become increasingly recognised as the cause of various infections. campylobacter jejuni and c coli cause acute gastroenteritis in man all over the world. c jejuni enteritis can lead to bacteraemia, but its actual incidence remains unknown. seven cases of bacteraemia caused by c jejuni or c coli are reported, from the blood of seven patients: five immune deficient adults; a newborn baby; and a patient who had had abdominal surgery. patients who develop diarrhoea as a r ... | 1994 | 8132835 |
| iron-regulated proteins in outer membranes of campylobacter jejuni diarrhoea isolates and immune response to the proteins in patients. | the outer membrane protein (omp) profiles from 8 campylobacter jejuni and 5 campylobacter coli fecal isolates grown under various conditions were compared by sds-page. the bacteria were grown under usual conditions, in iron-deficient medium (dip) and on iron-supplemented medium (fe). the omp profiles of most bacterial strains grown under usual conditions, or in the fe-supplemented medium, contained four major bands of approximately 31, 45, 63-66 and 97 kda, and in addition, a number of minor ban ... | 1994 | 8167428 |
| structural and antigenic characteristics of campylobacter coli flaa flagellin. | the polar flagellar filament of campylobacter coli vc167 is composed of two highly related (98%) flagellin subunit proteins, flaa and flab, whose antigenic specificities result from posttranslational modification. flaa is the predominant flagellin species, and mutants expressing only flaa form a full-length flagellar filament. although the deduced m(r) of type 2 (t2) flaa is 58,884 and the apparent m(r) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis is 59,500, the solution weight-a ... | 1994 | 7515043 |
| [an enzyme-linked immunosorbent assay for the detection of igg antibodies against urease of helicobacter pylori]. | we have developed an enzyme-linked immunosorbent assay (elisa) for the detection of igg antibodies against helicobacter pylori (hp) using purified hp urease as an antigen. the urease was purified from ultrasonicated extract of hp by nacl linear gradient system on deae-sepharose 4b chromatography. two molecular weight bands, 65kd and 27kd were observed on a sds-page gel in the purified urease sample. the urease antigen did not crossreact to rabbit antibodies prepared against campylobacter coli an ... | 1994 | 7844892 |
| cryptolepis sanguinolenta activity against diarrhoeal bacteria. | cryptolepine is the main alkaloid of cryptolepis sanguinolenta (lindl.) schlechter, a plant used in traditional medicine in west africa. the minimal inhibitory concentrations (mics) of cryptolepine, ethanol and aqueous extracts of cryptolepis sanguinolenta root were determined for 65 strains of campylobacter jejuni, 41 strains of campylobacter coli isolated from sporadic cases of gastroenteritis in portugal and 86 strains of vibrio cholerae isolated from patients with enteric infections in angol ... | 1994 | 7853867 |
| a pcr assay for the detection of campylobacter jejuni and campylobacter coli in water. | a pcr assay has been developed for the detection of campylobacter jejuni and camp. coli in water samples. the sample is filtered through a membrane which is subjected to sonication to release the impacted cells. after removal of the filter from the cell suspension and a freeze/thaw cell lysis step, a semi-nested pcr is carried out on the filtrate using the primers cf02, cf03 and cf04 (camp. jejuni flaa and flab gene sequences). incorporation of a sonication stage allows removal of the filter mem ... | 1994 | 7765441 |
| biotyping of campylobacter jejuni and campylobacter coli infections in spain. | biochemical profiles were studied in 102 campylobacter isolates from patients affected by enteric disease in orense (spain) over a 1-year period. the isolates were identified as hippurate-positive campylobacter jejuni (n = 90), campylobacter coli (n = 10) and hippurate-negative c. jejuni (n = 2). seventy-seven of the hippurate-positive and both hippurate-negative c. jejuni isolates were biotyped as c. jejuni subsp. jejuni biotype 1, nine as c. jejuni subsp. jejuni biotype 2 and four as c. jejuni ... | 1994 | 7884224 |
| seroreactivity to campylobacter jejuni and gangliosides in patients with guillain-barré syndrome. | seroreactivities to campylobacter jejuni or campylobacter coli and to the gangliosides gm1 and gd1b were studied by quantitative and immunoglobulin class-specific elisas in 42 patients with recent onset of acute guillain-barré syndrome (gbs), in 39 patients with positive campylobacter serology but no neurologic disease, and in 52 healthy blood donors. gbs patients showed positive reactivities to c. jejuni in 57%, 26%, and 2% for igg, iga, and igm, respectively, while blood donors had correspondi ... | 1994 | 7930724 |
| detection of flagellar antigen of campylobacter jejuni and campylobacter coli in canine faeces with an enzyme-linked immunosorbent assay (elisa)--new prospects for diagnosis. | a new diagnostic procedure was developed to detect the flagellar antigen of campylobacter jejuni and campylobacter coli in canine faecal specimens and was tested on faecal samples from random-source dogs obtained from the local dog pound. extraction of acid-soluble proteins was performed on faecal specimens and the extracted material was evaluated using species-specific monoclonal antibodies in an enzyme-linked immunosorbent assay. the assay detected all c. jejuni or c. coli infected specimens c ... | 1994 | 7975201 |
| cloning, nucleotide sequence and characterization of a gene encoding superoxide dismutase from campylobacter jejuni and campylobacter coli. | genes encoding superoxide dismutase (sod: ec 1.15.1.1) were cloned from campylobacter jejuni nctc 11351 and campylobacter coli ua585 by heterologous complementation of a sod-deficient escherichia coli mutant. deletion analysis of the cloned c. jejuni dna assigned the sod gene to a 1.2 kb insert and this contained an open reading frame of 660 bp. the deduced gene product of 220 amino acids was 71% identical to the e. coli iron-containing sod and 60% identical to the e. coli manganese-containing s ... | 1994 | 8025686 |
| phylogenetic and molecular characterization of a 23s rrna gene positions the genus campylobacter in the epsilon subdivision of the proteobacteria and shows that the presence of transcribed spacers is common in campylobacter spp. | the nucleotide sequence of a 23s rrna gene of campylobacter coli vc167 was determined. the primary sequence of the c. coli 23s rrna was deduced, and a secondary-structure model was constructed. comparison with escherichia coli 23s rrna showed a major difference in the c. coli rrna at approximately position 1170 (e. coli numbering) in the form of an extra sequence block approximately 147 bp long. pcr analysis of 31 other strains of c. coli and c. jejuni showed that 69% carried a transcribed space ... | 1994 | 8045890 |
| characterization of the sat4 gene encoding a streptothricin acetyltransferase in campylobacter coli be/g4. | the sat4 streptothricin resistance gene from campylobacter coli be/g4 was cloned into puc18, and its nucleotide sequence was determined. streptothricin acetyltransferase activity was detected in escherichia coli cells containing recombinant plasmid pat132 which carries the sat4 gene as an insert. the deduced amino acid sequence displayed 21-27% amino acid identity with streptothricin acetyltransferases from e. coli and streptothricin producers streptomyces lavendulae and streptomyces noursei. th ... | 1994 | 8056285 |
| macro-fingerprinting analysis at the chromosomal genomic dna level of isolates of thermophilic campylobacter coli and c. jejuni, by pulsed-field gel electrophoresis. | chromosomal genomic dna from 25 strains of thermophilic campylobacter coli and c. jejuni were analysed after pulsed-field gel electrophoresis (pfge) of restriction fragments generated by sali and smai to evaluate the utility of macro-fingerprinting analysis and to assess the possible subtyping of the isolates. two rare-cutting restriction enzymes, sali and smai, allowed the subclassification of eight strains of c. coli into six types. sali facilitated the subclassification of seventeen strains o ... | 1994 | 8070288 |
| distribution and polymorphism of the flagellin genes from isolates of campylobacter coli and campylobacter jejuni. | the complex flagellar filaments of the lio8 serogroup member campylobacter coli vc167 are composed of two highly related subunit proteins encoded by the flaa and flab genes which share 92% identity. using oligonucleotide primers based on the known dna sequence of both the flaa and flab genes from c. coli vc167 in the polymerase chain reaction, we have shown conservation of both fla genes among isolates within the lio8 heat-labile serogroup by digestion of the amplified product with psti and ecor ... | 1993 | 8098328 |
| azithromycin resistance in campylobacter jejuni and campylobacter coli. | the mics of erythromycin, azithromycin and ciprofloxacin were determined for 60 human fecal isolates of campylobacter. of these, 30 strains selected on the basis of their resistance to erythromycin by disk diffusion were highly resistant to both erythromycin and azithromycin. nine of these selected isolates were resistant to ciprofloxacin. the remaining 30 strains were non-selected, consecutive isolates of campylobacter susceptible to erythromycin by disk diffusion and were shown to be two- to f ... | 1993 | 8112360 |
| use of nonradioactive dna probes to identify a campylobacter jejuni strain causing abortion. | a case of human abortion due to a campylobacter infection is reported. cultures revealed two morphologically different isolates with large and small colonies respectively. using conventional methods of identification, the large colonies were identified as campylobacter jejuni and the small colonies as campylobacter coli. dot blot hybridization and determination of rdna restriction fragment patterns revealed that both colony types were the same strain of campylobacter jejuni. this observation ill ... | 1993 | 8223663 |
| transformation of helicobacter pylori by chromosomal metronidazole resistance and by a plasmid with a selectable chloramphenicol resistance marker. | most strains of helicobacter pylori are naturally competent for uptake of chromosomal dna. transformation frequencies for streptomycin resistance or rifampicin resistance markers ranged from 1 x 10(-4) to 1 x 10(-3) per viable cell using a plate transformation procedure. transformation of a metronidazole resistance marker (mtrr) was demonstrated when either a laboratory-derived mutant or a mtrr clinical isolate were used as the source of donor dna. mtrr was transformed at a frequency of 3 x 10(- ... | 1993 | 8254319 |
| [unusual infectious complication in a pregnant woman. spontaneous abortion caused by campylobacter coli]. | 1993 | 8259031 | |
| interaction of helicobacter pylori (strain 151) and campylobacter coli with human peripheral polymorphonuclear granulocytes. | helicobacter pylori associated gastritis is characterized by dense mucosal inflammatory infiltrations with predominantly neutrophilic granulocytes, together with a local and systemic immune response. nevertheless, the natural course of the infection is chronic in nature, and active phagocytosis of h. pylori by mucosal granulocytes was only rarely observed. the aim of the present study was to investigate with electronmicroscopic methods the interaction of h. pylori with freshly harvested human pe ... | 1993 | 8280958 |
| efficacy of filter types for detecting campylobacter jejuni and campylobacter coli in environmental water samples by polymerase chain reaction. | a previously developed polymerase chain reaction (pcr) amplification of a target region in the flaa campylobacter flagellin gene was evaluated and adapted for use with environmental water samples. the ability to detect campylobacter jejuni or campylobacter coli in seeded water samples was tested with various filters after concentration and freeze-thaw lysis of the bacterial cells. a nonradioactive probe for the amplified flagellin gene fragment detected as little as 1 to 10 fg of genomic dna and ... | 1993 | 8285708 |
| development of species-specific dna probes for campylobacter jejuni, campylobacter coli, and campylobacter lari by polymerase chain reaction fingerprinting. | the application of polymerase chain reaction (pcr) fingerprinting assays enables discrimination between species and strains of microorganisms. pcr primers aiming at arbitrary sequences in combination with primers directed against the repetitive extragenic palindrome (rep) or enterobacterial repetitive intergenic consensus (eric) motifs generate isolate-specific dna banding patterns. analysis of these pcr fingerprints obtained for 33 isolates of campylobacter jejuni, 30 isolates of campylobacter ... | 1993 | 8314996 |
| the campylobacter sigma 54 flab flagellin promoter is subject to environmental regulation. | the complex flagellum of campylobacter coli vc167 is encoded by two tandemly oriented flagellin genes which are transcribed as two discrete transcriptional units from two different classes of promoters. the flab gene, which encodes the minor flab filament protein, is controlled by a sigma 54 promoter. a transcriptional fusion between a promoterless chloramphenicol acetyltransferase (cat) reporter gene cartridge and c. coli vc167 dna carrying flab transcription and translation signals, including ... | 1993 | 8331072 |
| an antigenic polysaccharide from campylobacter coli serotype o:30. structure of a teichoic acid-like antigenic polysaccharide associated with the lipopolysaccharide. | a water-soluble antigenic polysaccharide of high m(r) associated with the lipopolysaccharide has been isolated from phenol-water extraction of cells of campylobacter coli serotype o:30. the polysaccharide and oligosaccharide degradation products formed on o-dephosphorylation and by periodate oxidation followed by reduction have been investigated by one- and two-dimensional 1h, 13c, and 31p nmr. it is concluded that the antigenic polysaccharide has a teichoic acid-like structure with a poly-ribit ... | 1993 | 8349707 |
| direct polymerase chain reaction detection of campylobacter jejuni and campylobacter coli in raw milk and dairy products. | a polymerase chain reaction (pcr) method designed to sensitively detect and identify campylobacter jejuni and campylobacter coli without the need for isolating and culturing strains is described. the intergenic sequence between the flagellin genes flaa and flab was amplified and characterized with a triple primer or seminested primer approach. a total of 50 bacterial strains, 27 of c. jejuni and c. coli and 23 of other species, were tested, giving no false-positive or false-negative results. the ... | 1993 | 8357250 |
| peb1, the major cell-binding factor of campylobacter jejuni, is a homolog of the binding component in gram-negative nutrient transport systems. | the protein peb1 (28 kda) is a common antigen and a major cell adherence molecule of campylobacter jejuni and campylobacter coli. we created a bank of chromosomal dna fragments of c. jejuni strain 81-176 using lambda gt11. screening this bank in escherichia coli y1090 cells with antibody raised against purified peb1 enabled us to isolate and to purify a clone with a 2.6-kilobase insert expressing an immunoreactive protein of 28 kda. dna sequencing revealed that the insert contains three complete ... | 1993 | 8360165 |
| [hematologic manifestations in campylobacter coli septicemia. apropos of a case]. | we report the case of a 71-year old male patient with a chronic lymphoid leukaemia of 10 years' duration who abruptly suffered deterioration of this general condition and haematological disturbances suggesting worsening of this blood disease (richter's syndrome). blood cultures demonstrated a campylobacter coli septicaemia. treatment with antibiotics resulted in a return to the previous clinical and biological situation. the various visceral manifestation of the infection are discussed. | 1993 | 8362107 |
| characterization of a new class of tetracycline-resistance gene tet(s) in listeria monocytogenes bm4210. | the nucleotide sequence of the tetracycline (tc)-minocycline (mc)-resistance determinant of plasmid pip811 from listeria monocytogenes bm4210 has been determined. the gene, designated tet(s), was identified by analysis of the start and stop codons as a coding sequence of 1923 bp, corresponding to a protein with a calculated m(r) of 72,912. the apparent 68-kda size estimated by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis of the protein characterized in a cell-free coupled transcript ... | 1993 | 8370538 |
| helicobacter acinonyx sp. nov., isolated from cheetahs with gastritis. | four strains of a novel helicobacter species were isolated from the stomachs of cheetahs (acinonyx jubilatus) with gastritis. these isolates were phenotypically similar to helicobacter pylori. the isolates were gram-negative, spiral bacteria which grew under microaerophilic conditions at 37 degrees c, but not at 25 or 42 degrees c, and produced urease, catalase, oxidase, alkaline phosphatase, and gamma-glutamyl transpeptidase. the isolates did not ferment glucose, mannitol, inositol, sorbitol, r ... | 1993 | 8379970 |
| cloning and nucleotide sequence of the campylobacter jejuni gyra gene and characterization of quinolone resistance mutations. | the gyra gene of campylobacter jejuni ua580, which encodes the a subunit of dna gyrase, was cloned and its nucleotide sequence was determined. an open reading frame of 2,589 nucleotides was identified, which could code for a polypeptide of 863 amino acids with a m(r) of 97 kda. both the nucleotide sequence and the putative amino acid sequence show ca. 50% identity with those of other gyra genes from gram-positive and gram-negative bacteria. the locations of the gyra gene on genome maps of both c ... | 1993 | 8384814 |
| susceptibilities of beta-lactamase-positive and -negative strains of campylobacter coli to beta-lactam agents. | the percentages of susceptibility of 28 strains of campylobacter coli to beta-lactam agents were 96% for amoxicillin and ampicillin, 57% for ticarcillin, 4% for cefoxitin and cefuroxime, 61% for cefotaxime, and 11% for ceftazidime. none of the strains were susceptible to penicillin g, piperacillin, cefazolin, cephalothin, cefamandole, and cefoperazone. all strains were susceptible to imipenem and ciprofloxacin, and 21% were susceptible to erythromycin. a beta-lactamase was detected in 68% of the ... | 1993 | 8390812 |
| heterologous gene expression in campylobacter coli: the use of bacterial luciferase in a promoter probe vector. | a novel promoter probe plasmid (psp73) was constructed to allow the analysis of environmentally regulated gene expression in campylobacter. the vector utilizes the luxab genes from xenorhabdus luminescens, which encode a thermostable luciferase, as reporters of gene expression. the utility of this reporter system was demonstrated by placing the expression of luxab under the transcriptional control of the flaa gene promoter. | 1993 | 8405932 |
| characterization of viable but nonculturable stage of c. coli, characterized with respect to electron microscopic findings, whole cell protein and lipooligosaccharide (los) patterns. | campylobacter coli ck 205, isolated from swine feces, was examined for changes in cell morphology, protein and lipooligosaccharide (los) patterns during starvation-survival experiments. bottles filled with sterile filtered a. dest. were seeded with campylobacters and incubated at 4 degrees c and 37 degrees c. transition to the nonculturable stage occurred within 48 hours (37 degrees c) and 2 weeks (4 degrees c), respectively. in contrast to the culturability the electrophoretic studies showed no ... | 1993 | 8451879 |
| effect of host lineage on the virulence of campylobacter jejuni/coli in the chick embryo model. | the chorioallantoic membrane (cam) inoculated chick embryo model was used to study the effect of host lineage on the virulence of campylobacter jejuni and campylobacter coli. ld50 values were used to compare the susceptibilities of chick embryos from eight inbred chicken lines to infection by four strains of c. jejuni and one strain of c. coli. differences in susceptibility were found between inbred chicken lines. these were shown not to be due to maternal antibody status, nor transfer of antibo ... | 1993 | 8454192 |
| lipopolysaccharide of campylobacter coli serotype o:30. fractionation and structure of liberated core oligosaccharide. | lipopolysaccharide from phenol-water extraction of cells of campylobacter coli serotype o:30 was separated as a water-insoluble gel of low m(r) from a water-soluble antigenic polysaccharide of high m(r). acetic acid hydrolysis of the ketosidic linkages to lipid a in the lipopolysaccharide furnished a core oligosaccharide. structural investigations were performed using 1h and 13c nmr, fast atom bombardment-mass spectrometry of permethylated derivatives, and methylation linkage analysis on the cor ... | 1993 | 8454598 |
| evaluation of commercial antisera for serotyping heat-labile antigens of campylobacter jejuni and campylobacter coli. | commercial antisera for serotyping 22 heat-labile antigens of campylobacter jejuni and campylobacter coli were evaluated by using 66 isolates from human and nonhuman sources. test results were compared with results of tests using antisera produced at the centers for disease control (cdc), atlanta, ga. all strains (three isolates of each of the 22 serotypes) were typeable with the cdc antisera. of 66 test strains, 39 (59%) were typed as the same serotype with both sets of antisera. twenty-four st ... | 1993 | 8463402 |
| significance of duplicated flagellin genes in campylobacter. | the complex flagellum of campylobacter coli vc167 contains two highly related (98%) flagellin subunit proteins which are produced from two 92% homologous, tandemly orientated genes, flaa and flab. mutants expressing only flaa form a full-length flagellar filament that confers slightly less than wild-type motility to the bacterium. however, flagellin mutants expressing only flab produce extremely short, truncated filaments, and are only slightly motile. we have shown that the presence of two esse ... | 1993 | 8464049 |
| the role of management systems in the epidemiology of thermophilic campylobacters among poultry in eastern zone of tanzania. | a total of 255 samples of droppings collected from a total of 22 different poultry units were examined for the presence of thermophilic campylobacters and the isolates biotyped using skirrow's protocol. the organisms were isolated from 90 (35.3%) of all samples. among the 22 units investigated, 13 (59%) were found to have unsatisfactory management systems, while 7 (32%) and 2 (9%) were found to have unsatisfactory and good systems respectively. significantly large numbers of isolations, 68 of 14 ... | 1993 | 8472771 |
| arcobacter (campylobacter) butzleri-associated diarrheal illness in a nonhuman primate population. | after dna hybridization identified an isolate from an ill rhesus macaque (macaca mulatta) as arcobacter (campylobacter) butzleri, we initiated a study to determine whether a. butzleri was associated with diarrheal disease in nonhuman primates at the yerkes primate research center. by using campy-cva medium incubated at 35 degrees c, 15 a. butzleri isolates were obtained from 14 macaques; 7 macaques were coinfected with campylobacter coli and campylobacter jejuni. a. butzleri was not isolated fro ... | 1993 | 8478115 |
| discrimination among thermophilic campylobacter species by polymerase chain reaction amplification of 23s rrna gene fragments. | by comparing nucleic acid sequences determined for one of the most variable areas of 23s rrna genes of 23 campylobacter strains, we were able to identify regions specific for thermophilic campylobacter strains. oligonucleotide primers corresponding to these unique regions were synthesized and used in the polymerase chain reaction. one primer pair selectively detected all thermophilic campylobacter species, while four other primer pairs allowed discrimination among the thermophilic species campyl ... | 1993 | 7508460 |
| common somatic o and heat-labile serotypes among campylobacter strains from sporadic infections in the united states. | somatic o (formerly heat-stable) and heat-labile (hl) serotyping methods are commonly used to type campylobacter jejuni and campylobacter coli isolates. although both systems are effective, the labor and time required for each have limited their application. these systems can be simplified by reducing the number of antisera used. to find an appropriate panel of antisera, we determined the distribution of common serotypes in the united states among a representative sample of 298 campylobacter iso ... | 1993 | 7686183 |
| direct polymerase chain reaction detection of campylobacter jejuni and campylobacter coli in raw milk and dairy products. | [this corrects the article on p. 2161 in vol. 59.]. | 1993 | 16349140 |
| rapid and sensitive detection of campylobacter spp. in chicken products by using the polymerase chain reaction. | the polymerase chain reaction (pcr) after a short enrichment culture was used to detect campylobacter spp. in chicken products. after the 16s rrna gene sequence of campylobacter jejuni was determined and compared with known sequences from other enterobacteria, a primer and probe combination was selected from the region before v3 and the variable regions v3 and v5. with this primer set and probe, 426-bp fragments from c. jejuni, campylobacter coli, and campylobacter lari could be amplified. the d ... | 1992 | 1282312 |
| emergence of resistance to erythromycin and fluoroquinolones in thermotolerant campylobacter strains isolated from feces 1987-1991. | during the period 1987 to 1991 a retrospective study was performed to determine the resistance of thermotolerant campylobacter species isolated from feces to erythromycin and fluoroquinolones. of the 672 strains studied, 614 (91.3%) were identified as campylobacter jejuni and 58 (8.7%) as campylobacter coli. during the study period the rate of resistance of campylobacter jejuni to erythromycin remained relatively stable (0.9-3.5%), while resistance of campylobacter coli to erythromycin emerged l ... | 1992 | 1291314 |
| genome maps of campylobacter jejuni and campylobacter coli. | little information concerning the genome of either campylobacter jejuni or campylobacter coli is available. therefore, we constructed genomic maps of c. jejuni ua580 and c. coli ua417 by using pulsed-field gel electrophoresis. the genome sizes of c. jejuni and c. coli strains are approximately 1.7 mb, as determined by sali and smai digestion (n. chang and d. e. taylor, j. bacteriol. 172:5211-5217, 1990). the genomes of both species are represented by single circular dna molecules, and maps were ... | 1992 | 1313002 |
| serotype distribution of campylobacter jejuni and campylobacter coli isolated from hospitalized patients with diarrhea in central australia. | campylobacter jejuni and/or campylobacter coli was cultured from 218 of 1,078 patients of all age groups admitted to alice springs hospital, alice springs, central australia, between july 1988 and june 1989 for treatment of diarrhea. one hundred sixty-six campylobacter colonies from 127 patients were subjected to o serotyping by using the penner typing scheme. all except 29 colonies could be serotyped. a total of 46 serotypes were identified, and the predominant serotypes were o:8, 17, o:22, o:1 ... | 1992 | 1370848 |
| biochemical and antigenic properties of the campylobacter flagellar hook protein. | the flagellar filament-hook complex was removed from campylobacter cells by shearing and was purified by differential solubilization and ultracentrifugation at ph 11 followed by cesium chloride buoyant density ultracentrifugation. flagellar filaments were then dissociated in 0.2 m glycine-hcl (ph 2.2), and purified hooks were collected by ultracentrifugation. the hooks (105 by 24 nm) each displayed a conical protrusion at the proximal end, a concave cavity at the distal end, and helically arrang ... | 1992 | 1375929 |
| ribosomal rna gene restriction fragment diversity amongst lior biotypes and penner serotypes of campylobacter jejuni and campylobacter coli. | diversity based on ribosomal rna gene-restriction endonuclease digest patterns was detected amongst 42 strains of campylobacter jejuni and 18 strains of c. coli including representatives of 53 different penner serotypes. haeiii ribopatterns were coded for numerical analysis which showed that all except two were different including those of several strains of the same serotype (p2 and p20). at the 30% similarity level, four groupings were formed in the analysis of which three corresponded to c. j ... | 1992 | 1381326 |
| specific detection of campylobacter jejuni and campylobacter coli by using polymerase chain reaction. | development of a routine detection assay for campylobacter jejuni and campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (pcr). an oligonucleotide primer pair from a conserved 5' region of the flaa gene of c. coli vc167 was used to amplify a 450-bp region by pcr. the primer pair specifically detected 4 strains of c. coli and 47 strains of c. jejuni; but it did not detect strains of campylobacter fetus, campylobacter lari, campylobacter upsaliensis, cam ... | 1992 | 1400961 |
| [chemotherapy of campylobacter infections]. | a total of 600 patients with suspected alimentary food poisoning were hospitalized. the ++clinico-laboratory findings showed that 27 (4.5 per cent) of them had campylobacter infection. the cultures of campylobacter jejuni and campylobacter coli were isolated from 24 and 3 patients, respectively. the patients underwent complex pathogenetic treatment with oral rehydration saline solutions, symptomatic agents, enzymatic preparations and diet (the basic therapy) supplemented with biological bacteria ... | 1992 | 1417342 |
| simple and economical culture of campylobacter jejuni and campylobacter coli in co2 in moist air. | strains of campylobacter jejuni and c. coli representing the 18 serogroups (lior) most commonly isolated from humans in canada were grown on solid media in an atmosphere of 10% co2 in moist air, 99% relative humidity. when the growth of all 18 serogroups on mueller hinton agar in a microaerobic atmosphere (5% o2, 10% co2 and 85% n2) was compared with the growth of all 18 serogroups on the same media in 10% co2 in moist air, colony sizes were significantly larger (p less than 0.05) for strains gr ... | 1992 | 1419543 |
| prevalence, biotypes, plasmid profile and antimicrobial resistance of campylobacter isolated from wild and domestic animals from northeast portugal. | the incidence of campylobacter jejuni and campylobacter coli in wild and producing animals has been studied to evaluate their importance as potential reservoirs of campylobacter infection. these organisms were isolated from: 59 chicken (60.2%), 65 swine (59.1%), 31 black rats (57.4%), 61 sparrows (45.5%), 21 ducks (40.5%), 32 cows (19.5%) and 27 sheep (15.3%). biotypes, plasmid and resistance profiles were studied in order to characterize the isolates. biotypes i and ii of c. jejuni were predomi ... | 1992 | 1429305 |
| cloning and genetic characterization of a helicobacter pylori flagellin gene. | helicobacter pylori produces polar sheathed flagella, which are believed to be essential for the bacterial colonization of the human gastric mucosa. here we report on the cloning and genetic characterization of a h. pylori gene encoding the subunit of the flagellar filament, the flagellin. screening of a genomic library of h. pylori with an oligonucleotide probe derived from the n-terminal amino acid sequence of purified flagellin resulted in a recombinant plasmid clone carrying the flagellin-en ... | 1992 | 1435261 |
| occurrence, removal and seasonal variation of "thermophilic" campylobacters in a sewage treatment plant in italy. | monitoring of "thermophilic" campylobacters in a sewage treatment plant in bologna (italy) has shown that incoming sewage contained a most probable number of 1630 campylobacters/100 ml. the secondary treatment in activated sludge tanks reduced 98.61% of campylobacters, 95.32% of fecal coliforms, 96.46% of fecal streptococci, 93.36% of salmonellas and 93.01% and 88.29% of bod5 and cod respectively. subsequent tertiary treatment with 3 ppm of chlorine dioxide for 15 min reduced 100% of campylobact ... | 1992 | 1457031 |
| structural studies of peptidoglycans in campylobacter species. | peptidoglycans (pg) from campylobacter coli, campylobacter jejuni, and campylobacter fetus were composed of muramic acid, glucosamine, alanine, glutamic acid, and diaminopimelic acid in a molar ratio of 1.1:1:1.7:1.1:09. thirty percent of the amino groups of diaminopimelic acid were involved in cross-linkages between peptides. during cultivation, c. coli and c. jejuni changed from a spiral to a coccoid form. in c. coli, we could isolate pg only from the spiral forms in yields of 0.8-1.2% by dry ... | 1992 | 1461153 |
| antibiotic susceptibility patterns and plasmid profile of helicobacter isolates from nigeria. | antibiotic susceptibility testing and plasmid screening of 50 local isolates of campylobacter coli and campylobacter jejuni were done. all isolates were sensitive to ofloxacin but were resistant to ampicillin, cloxacillin, penicillin, streptomycin and aztreonam. a large number of isolates were sensitive to nalidixic acid (96%), ceftriaxone (96%) and chloramphenicol (86%). it is noteworthy that nine (18%) of the isolates were resistant to each of erythromycin, 15 (30%) were found to harbour plasm ... | 1992 | 1476963 |
| antimicrobial susceptibilities of campylobacter jejuni and campylobacter coli isolated in sweden: a 10-year follow-up report. | resistance to erythromycin and doxycycline and more recently to fluoroquinolones has been reported to occur in campylobacter spp. both in vitro and in patients treated with these antibiotics. the frequency of resistance to 14 antimicrobial agents in campylobacter jejuni and campylobacter coli isolated from patients infected in sweden or abroad is described. for some agents, a comparison of susceptibility in strains of campylobacter spp. isolated in 1978 with those isolated in 1988 is made. no ge ... | 1992 | 1482158 |
| characterization of two plasmids from campylobacter jejuni isolates that carry the apha-7 kanamycin resistance determinant. | two small plasmids of 11.5 and 9.5 kb, each carrying an apha-7 kanamycin phosphotransferase gene, were studied. the mics of kanamycin for the two human campylobacter jejuni isolates harboring the plasmids were 10,000 and 5,000 micrograms/ml, while the mics of amikacin were 32 and 8 micrograms/ml, respectively. the mics of gentamicin and tobramycin were less than or equal to 2 micrograms/ml for both isolates. the restriction endonuclease maps of the plasmids were similar, with the larger plasmid ... | 1992 | 1503433 |
| a multiple logistic model for predicting the occurrence of campylobacter jejuni and campylobacter coli in water. | a multiple logistic regression model was established to predict the occurrence of campylobacter jejuni/coli, related to index bacteria such as faecal coliforms, faecal streptococci, and sulphite-reducing clostridia, in a water source in southern norway. the fitted model indicated that faecal coliforms were strong predictors for c. jejuni/coli, although the water temperature also had a strong influence on results. sulphite-reducing clostridia, faecal streptococci, and season of the year had no si ... | 1992 | 1512182 |
| a case of intrauterine fetal death associated with maternal campylobacter coli bacteraemia. | campylobacter species are known to cause infectious abortion in domestic animals. in humans, campylobacter are an important cause of enteritis, an occasional cause of systemic infection and have had a rare association with abortion and perinatal infection. a case history of spontaneous abortion, at 26 weeks' duration, associated with maternal bacteraemia, due to campylobacter coli is presented. transmission, pathogenesis, treatment, and the need for further investigation are discussed. | 1992 | 1520207 |
| experimental infection of the mouse mammary gland with campylobacter coli. | campylobacter coli strains of bovine and avian origin were inoculated into the mammary gland of mice. a bovine strain isolated from a case of mastitis produced gross and histological changes in most of the glands; one bovine and one avian faecal isolate did not. histologically, lesions were characterised by neutrophil infiltration in the alveolar spaces and necrosis and oedema in the interalveolar tissue. on bacteriological examination, the bovine mastitis strain could be isolated from most of t ... | 1992 | 1553432 |
| molecular discrimination of campylobacter coli serogroup 20 biotype i (lior) strains. | plasmid, protein and restriction endonuclease analysis (rea) profiles and multilocus enzyme electrophoresis were used to effect a molecular discrimination of twenty-seven campylobacter coli serogroup 20, biotype 1 (lior) strains. these strains were not outbreak-associated but were isolated from a number of different countries and different animal and environmental sources. each of the techniques was able to discriminate, to various degrees, between the serogroup 20, biotype 1 strains. the choice ... | 1992 | 1557899 |
| [occurrence of campylobacter coli in a central boar breeding facility in thuringia]. | investigating the cause of an outbreak of mild diarrhoea in a boar rearing unit with about 750 animals, c. coli was found in all diseased animals, i.e. it was isolated from the intestines of culled boars and from faeces samples; often the amount of c. coli was very high. the intragastric application of c. coli to 5 weaning pigs (about 35 kg) resulted in the shedding of large numbers of c. coli via the faeces at days 3 and 5 post infectionem only. the development of the animals was undisturbed du ... | 1992 | 1575669 |
| extended phage-typing scheme for campylobacter jejuni and campylobacter coli. | the extended phage-typing scheme described for campylobacter jejuni and campylobacter coli has established 46 different phage types using 19 typing phages. altogether 754 campylobacter isolates, 672 c. jejuni and 82 c. coli, isolated from human and non-human sources received from 17 different countries were phage-typed. overall, 80.6% of the total isolates were typable. among typable strains, 9 phage types (3, 5, 10, 11, 18, 19, 23, 26 and 44) represented 57.0% of the strains, 21.3% of the strai ... | 1992 | 1601075 |
| outer membrane components of campylobacter hyointestinalis. | outer membranes were isolated, by sodium lauryl sulphate extraction, from the american type strain, five australian, and four english isolates of campylobacter hyointestinalis. on sds-page examination, the protein profiles of seven strains (including the type strain) were similar, and were dominated by two major proteins of 47 and 50 kda. three other isolates had unique major protein profiles. the largest of these proteins was heat-modifiable in these isolates, and in the type strain. the flagel ... | 1992 | 1624127 |
| variation in antigenicity and molecular weight of campylobacter coli vc167 flagellin in different genetic backgrounds. | campylobacter coli vc167 has been shown to undergo a reversible flagellar antigenic variation between antigenic type 1 (t1) and antigenic type 2 (t2). vc167 contains two flagellin genes, and the products of both genes are incorporated into a complex flagellar filament in both antigenic types. although there are only minor amino acid changes in the flagellins expressed by t1 and t2 cells, the two antigenic types of flagellins can be distinguished by differences in apparent m(r) on sodium dodecyl ... | 1992 | 1624417 |
| antigenic shifts in serotype determinants of campylobacter coli are accompanied by changes in the chromosomal dna restriction endonuclease digestion pattern. | changes in somatic (o) lipopolysaccharide (lps) antigenic specificities of campylobacter coli serostrains were observed after continuous laboratory subculture. two serostrains (c. coli o34 and c. coli o48) lost o specificity and did not react with homologous or any of the available heterologous antisera. the c. coli serostrain for serogroup o5, after subculture, yielded a variant that had acquired a new specificity which was detectable with a heterologous antiserum. in a repeat experiment with t ... | 1991 | 1654431 |
| the in-vitro activity of temafloxacin compared with other antimicrobial agents. | temafloxacin, like other 4-quinolones, was highly active against most isolates of enterobacteriaceae. it was slightly less active than ciprofloxacin but approximately as active as ofloxacin and enoxacin. it was the most active of the quinolones against acinetobacter spp. and xanthomonas maltophilia but was slightly less active than ciprofloxacin against pseudomonas spp. temafloxacin was highly active against haemophilus influenzae, moraxella catarrhalis and neisseria gonorrhoeae and was the most ... | 1991 | 1657855 |
| in vitro susceptibilities of campylobacter jejuni and campylobacter coli to azithromycin and erythromycin. | mics of azithromycin and erythromycin for 20 campylobacter coli and 20 campylobacter jejuni strains were determined. the results demonstrated that, for campylobacter species, all high-level erythromycin-resistant strains were also resistant to azithromycin and that azithromycin did not exhibit increased potency in comparison with that of erythromycin. | 1991 | 1659309 |
| the in-vitro activity of tosufloxacin, a new fluorinated quinolone, compared with that of ciprofloxacin and temafloxacin. | tosufloxacin was highly active in vitro against most isolates of enterobacteriaceae. it was slightly less active than ciprofloxacin for most species but more active than temafloxacin. it was the most active agent against acinetobacter spp., xanthomonas maltophilia and some pseudomonas spp. but was slightly less active than ciprofloxacin against the fluorescent pseudomonads. tosufloxacin was highly active against strains of haemophilus influenzae, moraxella catarrhalis, neisseria gonorrhoeae and ... | 1991 | 1663930 |
| sizing and mapping of the genome of campylobacter coli strain ua417r using pulsed-field gel electrophoresis. | agarose-immobilized chromosomal dna from the nalidixic-acid-resistant campylobacter coli strain ua417 and its streptomycin-resistant (strr) derivative, ua417r, were digested with the restriction enzymes sali (gtcgac) and smai (cccggg). the sizes of the resulting fragments were determined using pulsed-field gel electrophoresis. the two genomes showed similar restriction patterns of seven and 13 fragments for the two respective enzymes and the total genome size was determined to be approx. 1.7 mb. ... | 1991 | 1676383 |
| a comparison of the in-vitro activity of clarithromycin, a new macrolide antibiotic, with erythromycin and other oral agents. | on the basis of minimum inhibitory concentrations clarithromycin (6-o-methylerythromycin), a new macrolide, was found to be slightly more active than erythromycin against staphylococcus aureus, enterococci. moraxella catarrhalis, gardnerella vaginalis, bacteroides fragilis (sensu stricto) and b. ureolyticus and slightly less active against coagulase-negative staphylococci, alpha- and beta-haemolytic streptococci, haemophilus influenzae, campylobacter coli/jejuni and the b. melaninogenicus/oralis ... | 1991 | 1684981 |
| identification, characterization, and spatial localization of two flagellin species in helicobacter pylori flagella. | flagellar filaments were isolated from helicobacter pylori by shearing, and flagellar proteins were further purified by a variety of techniques, including cscl density gradient ultracentrifugation, ph 2.0 acid disassociation-neutral ph reassociation, and differential ultracentrifugation followed by molecular sieving with a sephacryl s-500 column or mono q anion-exchange column, and purified to homogeneity by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transfer to an ... | 1991 | 1704004 |
| revision of campylobacter, helicobacter, and wolinella taxonomy: emendation of generic descriptions and proposal of arcobacter gen. nov. | hybridization experiments were carried out between dnas from more than 70 strains of campylobacter spp. and related taxa and either 3h-labeled 23s rrnas from reference strains belonging to campylobacter fetus, campylobacter concisus, campylobacter sputorum, campylobacter coli, and campylobacter nitrofigilis, an unnamed campylobacter sp. strain, and a wolinella succinogenes strain or 3h- or 14c-labeled 23s rrnas from 13 gram-negative reference strains. an immunotyping analysis of 130 antigens ver ... | 1991 | 1704793 |
| evaluation of a simplified procedure for serotyping campylobacter jejuni and campylobacter coli which is based on the o antigen. | a simplified procedure for serotyping campylobacter jejuni and campylobacter coli on the basis of thermostable antigens was developed and tested for its applicability as a routine typing method. the assay involves the sensitization of erythrocytes with an antigenic extract and performance of a slide agglutination assay with specific antisera. in order to simplify the typing system to a greater extent, the standard typing antisera were pooled into nine groups for c. jejuni and four groups for c. ... | 1991 | 1719020 |
| a dna sequence upstream of the tet(o) gene is required for full expression of tetracycline resistance. | the dna sequences upstream of the tet(o) and tet(m) open reading frames (orfs) (ca. 300 bp) were found to share a higher degree of homology than those of the tet(o) and tet(m) orfs themselves. a transcription initiation site for tet(o) was located by primer extension analysis. campylobacter coli was found to use a promoter sequence different from that used by escherichia coli. the sequence upstream of tet(o) was shown to be required in cis for high-level resistance to tetracycline. | 1991 | 1722078 |
| a note on the comparative efficacy of three selective media for isolation of campylobacter species from environmental samples. | the efficacy of three selective broths and agars, i.e. preston-, mccd- and car medium, were compared to each other in various combinations. twelve campylobacter coli and 9 campylobacter jejuni strains that had been isolated from sewage were used as test strains. to evaluate the applicability for highly contaminated environmental samples 15 sewage samples were examined, subsequently. significant differences between the media could not be ascertained. the application of preston broth/agar or car b ... | 1991 | 1750964 |
| fluoroquinolone resistance in campylobacter jejuni and campylobacter coli isolated from human faeces in switzerland. | 1991 | 1753149 | |
| potential sources of campylobacter species in the homes of farmworkers in zimbabwe. | chicken faeces, houseflies, household stored drinking water, cooked or prepared foods and handwashings of people of all age groups and hands of children under 5 years old were examined as potential sources and transmission routes of campylobacter species in a farmworker community. campylobacter species were found to be common in chicken faeces collected from the homesteads of the farmworkers but were rarely isolated from houseflies or household stored drinking water. they were not isolated from ... | 1991 | 1758009 |
| characterization of erythromycin resistance in campylobacter jejuni and campylobacter coli. | the mechanism of resistance to erythromycin, the drug of choice in the treatment of campylobacter gastroenteritis, was investigated. erythromycin resistance (mics, greater than 1,024 micrograms/ml) in three clinical isolates of campylobacter jejuni and one c. coli isolate was determined to be constitutive and chromosomally mediated. in vivo protein synthesis in erythromycin-susceptible c. jejuni and c. coli strains was completely inhibited by low levels of erythromycin (5 micrograms/ml), whereas ... | 1991 | 1759819 |
| emergence of fluoroquinolone resistance in campylobacter jejuni and campylobacter coli in subjects from finland. | the in vitro susceptibilities of 102 human campylobacter strains isolated between 1978 and 1980 and 100 strains isolated in 1990 to ciprofloxacin, norfloxacin, erythromycin, gentamicin, and doxycycline were examined. the biotypes and heat-stable serotypes of the strains as well as antimicrobial treatments and travel history of the campylobacter-positive patients were also studied. the results indicated that susceptibility to erythromycin, gentamicin, and doxycycline has remained the same during ... | 1991 | 1759828 |
| analysis of the role of flagella in the heat-labile lior serotyping scheme of thermophilic campylobacters by mutant allele exchange. | flagellin mutations originally constructed in campylobacter coli vc167 (serotype lio8) by a gene replacement mutagenesis technique (p. guerry, s. m. logan, s. thornton, and t. j. trust, j. bacteriol. 172:1853-1860, 1990) were moved from the original host into campylobacter strains of a number of other lior serogroups by a natural transformation procedure. this is the first report of the use of this transformation method to transfer a mutated locus among campylobacter strains. flagellin mutants w ... | 1991 | 1774247 |
| development of resistance to quinolones in five patients with campylobacteriosis treated with norfloxacin or ciprofloxacin. | development of resistance to nalidixic acid, norfloxacin and ciprofloxacin was observed in five patients with campylobacter jejuni or campylobacter coli infection. from all these patients nalidixic acid- and quinolone-susceptible strains were isolated initially, whereas after therapy with norfloxacin or ciprofloxacin strains resistant to these antibiotics were found. campylobacter strains from the same patient always belonged to the same species and, with the exception of one case, showed identi ... | 1991 | 1794366 |
| [morphological changes of campylobacter jejuni and campylobacter coli under various culture conditions and the accompanied changes in the cell composition]. | the morphological transformation from the spiral form to the coccoidal form campylobacter jejuni and campylobacter coli was studied under various conditions by such techniques as electron microscopy, sds-polyacrylamide gel electrophoresis (sds-page), and chemical analyses. the conversion from the spiral form to the coccoidal form of campylobacter in microaerophilic cultivation occurred in about 50% of the cells in 48 h and in about 90% of the cells in 72 h. at higher temperatures (37 c and 42 c) ... | 1991 | 1805043 |
| a noti restriction site of campylobacter coli chromosomal dna detected by double-digestion analysis with apai. | 1991 | 1832048 | |
| identification of campylobacter jejuni and campylobacter coli antigens with mucosal and systemic antibodies. | the development of a rapid and specific diagnostic assay for campylobacter infections is important in determining the etiology of acute diarrhea in humans. studies have shown that sonicated whole bacteria or partially purified antigens cross-reacted with antibodies against other closely related bacteria. to solve the problems of specificity, we identified specific antigens of campylobacter jejuni and campylobacter coli for use in diagnostic assays. we investigated the responses of serum, urine, ... | 1991 | 1855976 |
| role of two flagellin genes in campylobacter motility. | campylobacter coli vc167 t2 has two flagellin genes, flaa and flab, which share 91.9% sequence identity. the flaa gene is transcribed from a o-28 promoter, and the flab gene from a o-54 promoter. gene replacement mutagenesis techniques were used to generate flaa+ flab and flaa flab+ mutants. both gene products are capable of assembling independently into functional filaments. a flagellar filament composed exclusively of the flaa gene product is indistinguishable in length from that of the wild t ... | 1991 | 1856171 |
| incidence of toxigenic campylobacter strains in south africa. | campylobacter strains can produce a heat-labile cytotonic toxin (cton) and various cytotoxins (ctox). of 22 south african campylobacter strains tested, 86% were toxigenic (77% produced cton, 41% produced ctox, and 32% produced both types) and 14% were toxin negative. campylobacter jejuni strains were 67% cton positive and 47% ctox positive, whereas campylobacter coli strains were 100 and 29% positive, respectively. | 1991 | 1864947 |
| use of embryonating eggs for isolation of campylobacter species from intestines of swine with proliferative enteritis. | intestinal tissues from swine affected with proliferative enteritis were ground, filtered through a 0.65-micron pore membrane filter, diluted, and injected into 7-day-old embryonated hens' eggs via the yolk sac. at 2, 4, and 7 days later, yolk sac swab specimens taken from live embryos were cultured for campylobacter species. campylobacter hyointestinalis was recovered from eggs injected with tissues of swine with acute hemorrhagic proliferative enteritis at dilutions up to 10(-4). campylobacter ... | 1991 | 1883083 |
| identification, purification, and characterization of major antigenic proteins of campylobacter jejuni. | evidence from developing countries and volunteer studies indicates that immunity to campylobacter jejuni and campylobacter coli may be acquired, but the antigenic basis for this protection is poorly defined. we have purified to homogeneity four proteins with molecular weights of 28,000 (peb1), 29,000 (peb2), 30,000 (peb3), and 31,000 (peb4) from epidemic c. jejuni strain 81-176 using acid extraction and sequential ion-exchange, hydrophobic interaction, and gel filtration chromatography. the rela ... | 1991 | 1885571 |
| comparison of the survival of campylobacter jejuni and campylobacter coli in culturable form in surface water. | six campylobacter jejuni and six campylobacter coli strains were isolated from cows and pigs, and their survival in lake water was compared by viable counts. campylobacter jejuni survived longer in culturable form than c. coli in untreated and membrane-filtered water both at 4 and 20 degrees c. this difference in survival time may be a reason why c. jejuni is generally isolated from surface waters more frequently than c. coli. both species survived better in filtered than in untreated water. thi ... | 1991 | 1913357 |