Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
an outbreak of infectious hepatitis in commercially reared ostriches associated with campylobacter coli and campylobacter jejuni.a disease causing high morbidity and mortality was observed in young ostriches from six properties in southeast queensland, australia. the disease affected birds from 2-8 weeks of age and was characterised clinically by bright-green urates and pathologically by severe necrotic hepatitis. the liver lesions resembled those of vibrionic hepatitis in other avian species. campylobacter coli was isolated from the livers of affected ostriches from five of the six properties. campylobacter jejuni subsp. ...19989631530
heterogeneity in levels of vacuolating cytotoxin gene (vaca) transcription among helicobacter pylori strains.broth culture supernatants from tox+ helicobacter pylori strains induce vacuolation of hela cells in vitro and contain vaca in concentrations that are higher than those found in supernatants from tox- h. pylori strains. to investigate the basis for this phenomenon, we analyzed the transcription of the vacuolating cytotoxin gene (vaca) in eight tox+ strains (each with a type s1/m1 vaca genotype) and nine tox- strains (each with a type s2/m2 vaca genotype). most of the tox+ and tox- strains tested ...19989632570
isolation and molecular analysis of colonising and non-colonising strains of campylobacter jejuni and campylobacter coli following experimental infection of young chickens.fourteen-day-old chickens were inoculated with selected campylobacter coli and c. jejuni strains. c. jejuni strains were of two subgroups based on a polymorphism detected using a dna probe and represented the profiles typical for the majority of strains of either chicken or human origin. all c. coli strains previously isolated from humans colonised chickens, whereas from 4/7 c. jejuni strains of human origin, failed to colonise. of 12 campylobacter strains of chicken origin, 10 established a per ...19989646454
epidemiological typing of campylobacter isolates from meat processing plants by pulsed-field gel electrophoresis, fatty acid profile typing, serotyping, and biotyping.campylobacter spp. are a leading cause of bacterial gastroenteritis. foods of animal origin, particularly under-cooked poultry, are common sources of campylobacter species associated with disease in humans. a collection of 110 campylobacter jejuni and 31 c. coli human and environmental isolates from different ontario, canada, abattoirs were analyzed by pulsed-field gel electrophoresis, fatty acid profile typing, and biotyping. previously collected serotyping data for the same isolates were also ...19989647797
microtiter assay for detecting campylobacter spp. and helicobacter pylori with surface gangliosides which bind cholera toxin.campylobacter jejuni with gm1 ganglioside in the core of its lipopolysaccharide has been associated with guillain-barré syndrome. since this epitope may be of considerable pathophysiologic importance and since this ganglioside binds cholera toxin, a rapid screening assay to detect bacteria that bind cholera toxin as an indication of gm1 on their surfaces was developed. in the assay, bacterial lawns were grown on agar plates, harvested with phosphate-buffered saline, boiled, and incubated with a ...19989650959
the central, surface-exposed region of the flagellar hook protein flge of campylobacter jejuni shows hypervariability among strains.in a previous study, we observed that monoclonal antibodies raised against the hook protein flge of campylobacter jejuni lio 36, isolate 5226, bound exclusively to this strain. the aim of this study was to elucidate the molecular basis for these binding specificities. the hook protein-encoding gene flge of c. jejuni was cloned in escherichia coli and sequenced. the flge genes of four additional c. jejuni strains were amplified by pcr and also sequenced. comparison of the deduced amino acid seque ...19989658019
antimicrobial susceptibility testing of 59 strains of campylobacter fetus subsp. fetus.the susceptibilities of 59 campylobacter fetus subsp. fetus isolates to eight antibiotics were studied by the agar dilution, e-test, and disk diffusion methods. none of the isolates were beta-lactamase producers. all were susceptible to ampicillin, gentamicin, imipenem, and meropenem as determined by the three methods, with mics at which 90% of the isolates are inhibited (mic90s) (determined by agar dilution) of 2, 1, < or = 0.06, and 0.12 microgram/ml, respectively. twenty-seven percent of the ...19989661033
campylobacter upsaliensis: waiting in the wings.despite strong epidemiological evidence supporting an important role for campylobacter upsaliensis as a human enteropathogen, it remains relatively unknown in the realm of clinical microbiology. clinical studies indicate that infection with this organism usually is associated with benign self-limiting diarrhea. however, more serious illnesses, including spontaneous abortion and hemolytic-uremic syndrome, recently have been associated with human infections. understanding of the virulence properti ...19989665977
campylobacter species and guillain-barré syndrome.since the eradication of polio in most parts of the world, guillain-barré syndrome (gbs) has become the most common cause of acute flaccid paralysis. gbs is an autoimmune disorder of the peripheral nervous system characterized by weakness, usually symmetrical, evolving over a period of several days or more. since laboratories began to isolate campylobacter species from stool specimens some 20 years ago, there have been many reports of gbs following campylobacter infection. only during the past f ...19989665983
etiological study of diarrheal patients in vientiane, lao people's democratic republic.the etiological agents of diarrhea in vientiane, lao people's democratic republic (lao pdr), were studied in the period from october 1996 to august 1997. a total of 880 patients with diarrhea visiting medical facilities were examined for shigella, salmonella, diarrheagenic escherichia coli, vibrio, aeromonas, campylobacter, and rotavirus. shigella spp., heat-stable enterotoxin (st)-producing e. coli, and serogroup-based enteropathogenic e. coli were found to be the main organisms causing diarrhe ...19989665990
associations between heat-stable (o) and heat-labile (hl) serogroup antigens of campylobacter jejuni: evidence for interstrain relationships within three o/hl serovars.a comparative examination of the heat-stable (o) and heat-labile (hl) serogrouping results for 9,024 sporadic human isolates of campylobacter jejuni revealed conserved associations between specific o and hl antigens (o/hl serovars). forty-nine percent of the isolates which grouped for both o and hl antigens belonged to one of three serovars: o 4 complex/hl 1 (17.9%), o 1/hl 2 (16.8%), or o 50/hl 7 (14.5%). other common serovars were o 2/hl 4 (8.3%), o 6/hl 6 (8.1%), o 53/hl 11 (4.5%), o 19/hl 17 ...19989665996
lipopolysaccharides from campylobacter jejuni o:41 strains associated with guillain-barré syndrome exhibit mimicry of gm1 ganglioside.three campylobacter jejuni, biotype 2, serotype o:41 strains that were isolated from patients who developed guillain-barré syndrome (gbs) and one c. jejuni isolate from a patient who developed enteritis only were examined. the aim of the study was to determine the structure of the core oligosaccharide (os) of the lipopolysaccharide (lps) of c. jejuni serotype o:41, a serotype rarely associated with the development of gbs, and to determine if the lps shares similar epitopes with any of the major ...19989673245
phospholipase a of yersinia enterocolitica contributes to pathogenesis in a mouse model.some isolates of yersinia enterocolitica exhibit phospholipase activity, which has been linked to lecithin-dependent hemolysis (m. tsubokura, k. otsoki, i. shimohira, and h. yamamoto, infect. immun. 25:939-942, 1979). a gene encoding y. enterocolitica phospholipase was identified, and analysis of the nucleotide sequence revealed two tandemly transcribed open reading frames. the first, ypla, has 74% identity and 85% similarity to the phospholipase a found in serratia liquefaciens. though the othe ...19989673284
incidence and clinical manifestations of campylobacter enteritis in central taiwan.campylobacter species are recognized as a major cause of acute gastroenteritis in humans throughout the world and are increasingly associated with the development of guillain-barré syndrome. the age distribution of persons and the seasonal patterns of campylobacter infection are different in many developed and developing countries. the present study reports the investigation of the clinical and epidemiologic features of campylobacter infections in central taiwan.19989684510
antimicrobial resistance of clinical strains of campylobacter jejuni subsp. jejuni isolated from 1985 to 1997 in quebec, canada.the antimicrobial resistance of 158 campylobacter jejuni strains isolated from humans in quebec, canada, from 1995 to 1997 was compared to the resistance of 47 and 86 strains of c. jejuni isolated in 1985 and 1986 and in 1992 and 1993, respectively. of the 291 c. jejuni strains tested, no strain was resistant to erythromycin. compared to the c. jejuni strains isolated in 1985 and 1986, the c. jejuni strains isolated in 1992 and 1993 were more resistant to tetracycline (40.7 versus 19.1%, respect ...19989687415
mammalian cytidine 5'-monophosphate n-acetylneuraminic acid synthetase: a nuclear protein with evolutionarily conserved structural motifs.sialic acids of cell surface glycoproteins and glycolipids play a pivotal role in the structure and function of animal tissues. the pattern of cell surface sialylation is species- and tissue-specific, is highly regulated during embryonic development, and changes with stages of differentiation. a prerequisite for the synthesis of sialylated glycoconjugates is the activated sugar-nucleotide cytidine 5'-monophosphate n-acetylneuraminic acid (cmp-neu5ac), which provides a substrate for golgi sialylt ...19989689047
the lipopolysaccharide biosynthesis locus of campylobacter jejuni 81116.most campylobacter jejuni strains express lipo-oligosaccharides. some strains also express lipopolysaccharides (lps), with o-antigen-like carbohydrate repeats. c. jejuni 81116 expresses an lps containing both lipo-oligosaccharides and o-antigen-like repeats, but nothing is known about the structure or sugar composition of these lps species. a cosmid library of the genome of c. jejuni 81116 was constructed and probed with campylobacter hyoilei genes involved in lps synthesis. five cosmids hybridi ...19989720026
metronidazole resistance and microaerophily in campylobacter species.metronidazole is active against most anaerobic organisms and is also used in the treatment of the microaerophilic bacterium helicobacter pylori. resistance to metronidazole is uncommon in most anaerobic organisms, but it is increasingly prevalent in h. pylori. previously we have suggested that metronidazole resistance in h. pylori is inherent in the microaerophilic nature of the organism and therefore would be present in other microaerophiles such as campylobacter. short periods of anaerobiosis ...19989732442
in vitro genotypic variation of campylobacter coli documented by pulsed-field gel electrophoretic dna profiling: implications for epidemiological studies.six isolates of campylobacter coli from different pig herds were subcultured up to 50 times over a 6-month period and dna samples suitable for pulsed-field gel electrophoretic (pfge) profiling prepared at regular (1, 20, 40 and 50 passages) intervals. in 5/6 strains, changes in the banding patterns of sma1, sal1 and/or bamh1 digests were observed. in one such strain the differences were considered artifactual. however, significant alterations in pfge profiles between subcultures of four strains ...19989742707
resistance to antimicrobial agents used for animal therapy in pathogenic-, zoonotic- and indicator bacteria isolated from different food animals in denmark: a baseline study for the danish integrated antimicrobial resistance monitoring programme (danmap).this study describes the establishment and first results of a continuous surveillance system of antimicrobial resistance among bacteria isolated from pigs, cattle and broilers in denmark. the three categories of bacteria tested were: 1) indicator bacteria (escherichia coli, enterococcus faecalis, enterococcus faecium), 2) zoonotic bacteria (campylobacter coli/jejuni, salmonella enterica, yersinia enterocolitica), and 3) animal pathogens (e. coli, staphylococcus aureus, coagulase-negative staphyl ...19989744762
nucleotide sequence and characterization of cdra, a cell division-related gene of helicobacter pylori.we identified cell division-related gene cdra in helicobacter pylori hpk5. the putative gene product, cdra, is a 367-amino-acid polypeptide that exhibited a high level of homology to conserved hypothetical atp-binding protein hp0066 of h. pylori 26695, except in the n-terminal region, and showed some similarity to the ftsk/spoiiie family proteins. we isolated a cdra-disrupted mutant by allelic exchange mutagenesis. because of the low transformation frequency, the possibility that a suppressing m ...19989748467
identification of a gene encoding a methyl-accepting chemotaxis-like protein form campylobacter coli and its use in a molecular typing scheme for campylobacters.using pcr amplification with degenerate primers, a gene (tlpa) form campylobacter coli encoding a putative 63x0 kda polypeptide which exhibited significant identity with bacterial methyl-accepting chemotaxis proteins (mcps) was identified. a mutant containing an inactivated copy of the tlpa a gene showed a wild-type chemotactic response to all of the chemo-attractants tested. a dna probe based on the highly conserved domain (hcd) of tlpa revealed the presence of multiple copies of genes encoding ...19989750306
physiological activity of campylobacter jejuni far below the minimal growth temperature.the behavior of campylobacter jejuni at environmental temperatures was examined by determining the physiological activities of this human pathogen. the minimal growth temperatures were found to be 32 and 31 degrees c for strains 104 and atcc 33560, respectively. both strains exhibited a sudden decrease in growth rate from the maximum to zero within a few degrees not only near the maximal growth temperature but also near the minimal growth temperature. this could be an indication that a temperatu ...19989758819
effective recovery of bacterial dna and percent-guanine-plus-cytosine-based analysis of community structure in the gastrointestinal tract of broiler chickens.a dna-based, direct method for initial characterization of the total bacterial community in ileum and cecum of the chicken gastrointestinal (gi) tract was developed. the efficiencies of bacterial extraction and lysis were >95 and >99%, respectively, and therefore the dna recovered should accurately reflect the bacterial communities of the ileal and cecal digesta. total bacterial dna samples were fractionated according to their percent g+c content. the profiles reflecting the composition of the b ...19989758849
use of a selective medium and a membrane filter method for isolation of campylobacter species from spanish paediatric patients.a study was conducted to assess the value of a combination of two culture methods for isolation of campylobacter spp. from spanish children. seven hundred twenty-nine diarrhoeal stool specimens from 599 patients were examined for campylobacter spp. by culturing them on charcoal cefoperazone deoxycholate agar and on blood agar with a membrane filter. one hundred sixteen campylobacter strains were isolated from a total of 108 specimens; 75 (64.6%) were campylobacter jejuni, 32 (27.5%) were campylo ...19989764551
iron-responsive gene regulation in a campylobacter jejuni fur mutant.the expression of iron-regulated systems in gram-negative bacteria is generally controlled by the fur protein, which represses the transcription of iron-regulated promoters by using fe2+ as a cofactor. mutational analysis of the campylobacter jejuni fur gene was carried out by generation of a set of mutant copies of fur which had a kanamycin or chloramphenicol resistance gene introduced into the regions encoding the n and c termini of the fur protein. the mutated genes were recombined into the c ...19989765558
[relapsing campylobacter coli bacteremia in a hypogammaglobulinemic patient]. 19989767813
detection of point mutations associated with resistance of helicobacter pylori to clarithromycin by hybridization in liquid phase.when the standard procedure for determining antibiotic susceptibility of bacteria is used, the results are delayed, especially for bacteria that grow slowly, such as helicobacter pylori. treatment for this bacterium may involve clarithromycin, a compound for which resistance has been associated with point mutations on the 23s rrna gene. this resistance is currently found in organisms isolated from 0 to 15% of patients and jeopardizes the success of the treatment. we have designed a test involvin ...19989774580
a reverse transcriptase polymerase chain reaction assay for the detection of thermophilic campylobacter spp.a novel method was developed for the detection of thermophilic enteropathogenic campylobacters based on the detection of mrna using the reverse transcriptase polymerase chain reaction (rt-pcr). the rna extraction method, dnase treatment and rt-pcr assay were shown to be specific for mrna. the assay is specific for the thermophilic campylobacters campylobacter jejuni, campylobacter coli and campylobacter upsaliensis and restriction fragment length polymorphism (rflp) analysis of the 256 bp amplif ...19989778457
deletion of the major proteolytic site of the helicobacter pylori cytotoxin does not influence toxin activity but favors assembly of the toxin into hexameric structures.the helicobacter pylori cytotoxin is proteolytically cleaved at a flexible hydrophilic loop into two subunits. deletion of the loop sequences had no effect on biological activity of the toxin in the hela cell vacuolation assay but favored the organization of the protein into hexameric rather than heptameric structures.19989784571
cloning, sequencing, and characterization of the lipopolysaccharide biosynthetic enzyme heptosyltransferase i gene (waac) from campylobacter jejuni and campylobacter coli.campylobacter jejuni and campylobacter coli are common causes of gastrointestinal disease and a proportion of c. jejuni infections have been shown to be associated with the guillain-barré syndrome. the waac gene from campylobacter coli, involved in lipopolysaccharide core biosynthesis, was cloned by complementation of a heptose-deficient strain of salmonella typhimurium, as judged by novobiocin sensitivity, lipopolysaccharide (lps)-specific phage sensitivity, and polyacrylamide-resolved lipopoly ...19989831648
high-level resistance to trimethoprim in clinical isolates of campylobacter jejuni by acquisition of foreign genes (dfr1 and dfr9) expressing drug-insensitive dihydrofolate reductases.the pathogenic bacterium campylobacter jejuni has been regarded as endogenously resistant to trimethoprim. the genetic basis of this resistance was characterized in two collections of clinical isolates of c. jejuni obtained from two different parts of sweden. the majority of these isolates were found to carry foreign dfr genes coding for resistant variants of the dihydrofolate reductase enzyme, the target of trimethoprim. the resistance genes, found on the chromosome, were dfr1 and dfr9. in abou ...19989835491
rapid emergence of high-level resistance to quinolones in campylobacter jejuni associated with mutational changes in gyra and parc.quinolone resistance in clinical isolates of campylobacter jejuni in sweden increased more than 20-fold at the beginning of the 1990s. resistance to 125 microgram of ciprofloxacin per ml in clinical isolates was associated with chromosomal mutations in c. jejuni leading to a thr-86-ile substitution in the gyra product and a arg-139-gln substitution in the parc product.19989835526
effect of low-osmolality nutrient media on growth and culturability of campylobacter species.the growth and culturability of campylobacter jejuni nctc 11351 and other campylobacters were examined in media having different osmolalities at a range of temperatures (4, 25, and 42 degreesc). the medium osmolalities used ranged from the osmolality of full-strength nutrient medium (modified campylobacter broth having an osmolality of around 254 mosmol) down to 96 mosmol. the following two methods were used to produce media having different osmolalities: dilution of the nutrient medium with dis ...19989835543
a case control study of potential enteric pathogens for calves raised in cow-calf herds.a matched case control study was performed to describe the epidemiological features of potential enteric pathogens for calves reared in 53 cow-calf herds located in western switzerland. a total of 106 diarrhoeic calves and 126 healthy control calves were collected, all calves were less than 4 months old. faecal samples were analysed for presence of infectious agents related to calf diarrhoea including enterotoxigenic e. coli, verotoxin producing e. coli (vtec), campylobacter sp., yersinia sp., s ...19989852767
short-sequence dna repeats in prokaryotic genomes.short-sequence dna repeat (ssr) loci can be identified in all eukaryotic and many prokaryotic genomes. these loci harbor short or long stretches of repeated nucleotide sequence motifs. dna sequence motifs in a single locus can be identical and/or heterogeneous. ssrs are encountered in many different branches of the prokaryote kingdom. they are found in genes encoding products as diverse as microbial surface components recognizing adhesive matrix molecules and specific bacterial virulence factors ...19989618442
specific identification of the enteropathogens campylobacter jejuni and campylobacter coli by using a pcr test based on the ceue gene encoding a putative virulence determinant.a pcr method for the rapid identification and discrimination of thermophilic campylobacter jejuni and campylobacter coli was developed by using a gene encoding a protein involved in siderophore transport (ceue). a nucleotide sequence divergence of approximately 13% in the ceue genes of c. jejuni and c. coli facilitated the design of two species-specific pcr primer sets. the specificity of the pcr amplification reactions was confirmed by using two nonradioactively labelled species-specific intern ...19979041429
integration of heterologous plasmid dna into multiple sites on the genome of campylobacter coli following natural transformation.the efficiency of homologous recombination in campylobacter coli following the introduction of dna by natural transformation was determined by using a series of nonreplicating integrative vectors containing dna fragments derived from the c. coli catalase gene. homologous recombination occurred with as little as 286 homologous bp present and was not detected when 270 bases of homology was provided. instead, when plasmids with little or no homology to the chromosome were introduced by natural tran ...19979045845
campylobacter-host cell interactions.the enteric pathogens campylobacter jejuni and campylobacter coli are a major cause of infectious diarrhoea. their ability to adhere to human epithelial cells is ubiquitous and their propensity to invade cells is also well documented and requires motility and de novo protein synthesis, as well as several host factors. the molecular basis of the interaction between campylobacters and host cells is only beginning to be elucidate. the characteristics of this interaction promise to be interesting an ...19979080607
molecular characterization of plda, the structural gene for a phospholipase a from campylobacter coli, and its contribution to cell-associated hemolysis.a gene (plda) encoding a 35.0-kda protein with significant homology to the escherichia coli outer membrane phospholipase was identified upstream of an operon encoding an enterochelin transport system in campylobacter coli. the results of this study suggest that this gene encodes an outer membrane phospholipase a in c. coli. first, expression of the plda gene product in a plda-deficient mutant of e. coli led to the restoration of phospholipase a activity. the recombinant product also partitioned ...19979119448
the serotype distribution of campylobacter jejuni and c. coli isolated from patients with diarrhoea and controls at tikur anbassa hospital, addis ababa, ethiopia.sixty-eight isolates of campylobacter jejuni and c. coli isolated from patients with diarrhoea (n = 630) and controls (n = 220) at tikur anbassa hospital, addis ababa, ethiopia were serotyped on the basis of the heat-labile (hl) and the heat-stable (hs) antigens, by using 16 and 34 antisera, respectively, for the two methods. with the antisera against heat labile antigens, 89.3% of the c. jejuni and 75% of the c. coli were typable. the hl serotypes 1, 2, 3, 4, 5, 6 and 7 were the most common amo ...19979129584
comparison of a novel microaerobic system with three other gas-generating systems for the recovery of campylobacter species from human faecal samples.three commercial gas-generating systems--campygen (oxoid, uk), oxoid br56 (oxoid, uk), and campypak plus (becton dickinson, usa)--and the evacuation replacement technique were compared for the recovery of campylobacter spp. from 500 human faecal samples collected from patients with gastroenteritis. four hundred fifty faecal samples were tested upon receipt in the laboratory. fifty faecal samples that had been previously found to be positive for campylobacter spp. were tested retrospectively; the ...19979447907
norfloxacin resistance in campylobacter jejuni and campylobacter coli isolates from swedish patients.this study focused on the frequency of campylobacter jejuni and campylobacter coli strains resistant to norfloxacin. included were 1659 consecutive stool specimens isolated between 1992 and 1995, from as many swedish patients with diarrhoea. the patients were divided with regard to place of infection and age. all strains were tested for susceptibility to norfloxacin by means of disc diffusion test on blood-agar plates. norfloxacin-resistant strains (n = 310) were furthermore tested for resistanc ...19979301992
evaluation of antibodies reactive with campylobacter jejuni in egyptian diarrhea patients.serum and stool samples were collected from 128 individuals: 96 diarrhea patients and 32 apparently healthy controls. stool specimens were cultured for enteric bacterial pathogens, while sera were screened by enzyme-linked immunosorbent assay for campylobacter jejuni-reactive antibodies. of 28 diarrhea patients who demonstrated c. jejuni-reactive antibodies (titers, > 100), 14 were culture positive for this organism. the 32 healthy controls showed significantly lower antibody titers (p < 0.05) w ...19979302201
pcr detection, identification to species level, and fingerprinting of campylobacter jejuni and campylobacter coli direct from diarrheic samples.three sets of primers were designed for pcr detection and differentiation of campylobacter jejuni and campylobacter coli. the first pcr assay was designed to coidentify c. jejuni and c. coli based on their 16s rrna gene sequences. the second pcr assay, based on the hippuricase gene sequence, identified all tested reference strains of c. jejuni and also strains of that species which lack detectable hippuricase activity. the third pcr assay, based on the sequence of a cloned (putative) aspartokina ...19979316909
antimicrobial susceptibility patterns of thermophilic campylobacter spp. from humans, pigs, cattle, and broilers in denmark.the mics of 16 antimicrobial agents were determined for 202 campylobacter jejuni isolates, 123 campylobacter coli isolates, and 6 campylobacter lari isolates from humans and food animals in denmark. the c. jejuni isolates originated from humans (75), broilers (95), cattle (29), and pigs (3); the c. coli isolates originated from humans (7), broilers (17), and pigs (99); and the c. lari isolates originated from broilers (5) and cattle (1). all isolates were susceptible to apramycin, neomycin, and ...19979333055
campylobacter hyoilei alderton et al. 1995 and campylobacter coli véron and chatelain 1973 are subjective synonyms.the taxonomic affiliation of campylobacter hyoilei was reevaluated by examining a variety of phenotypic and genotypic criteria. whole-cell protein electrophoresis and a comparison of 66 phenotypic characters revealed that reference strains of c. hyoilei were indistinguishable from campylobacter coli strains. these data were confirmed by a dna-dna hybridization level of 67% between the type strains of the two species. several species-specific assays based on pcr amplification or probe hybridizati ...19979336905
specific detection and confirmation of campylobacter jejuni by dna hybridization and pcr.conventional detection and confirmation methods for campylobacter jejuni are lengthy and tedious. a rapid hybridization protocol in which a 1,475-bp chromogen-labelled dna probe (pdt1720) and campylobacter strains filtered and grown on 0.22-micron-pore-size hydrophobic grid membrane filters (hgmfs) are used was developed. among the environmental and clinical isolates of c. jejuni, campylobacter coli, campylobacter jejuni subsp. doylei, campylobacter lari, and arcobacter nitrofigilis and a panel ...19979361442
nucleotide sequence of the staphylococcus aureus transposon, tn5405, carrying aminoglycosides resistance genes.tn5405 is a 12 kb staphylococcal composite transposon delimited by two inverted copies of the insertion sequence is1182. this transposon carries two aminoglycosides resistance genes, apha-3 and aade, an altered gene similar to sat4 from campylobacter coli be/g4, and three open reading frames of unknown functions.19979373952
a genetic locus involved in iron utilization unique to some campylobacter strains.two genes involved in iron utilization in campylobacter coli vc167 t1 have been characterized. the cfra gene encodes a protein with a predicted mr of 77,653 which, after processing of the leader sequence, has a predicted mr of 75,635. this protein has significant sequence identity to siderophore receptors of several bacteria, and site-specific mutants defective in cfra do not synthesize one of two major iron-repressible outer membrane proteins. an adjacent gene encodes a tonb-like protein; a mut ...19979190817
comparison of disc diffusion and agar dilution methods for antibiotic susceptibility testing of campylobacter jejuni subsp. jejuni and campylobacter coli.the correlation between disc diffusion and agar dilution susceptibility testing of five antibiotics was studied against 145 campylobacter strains: 99 campylobacter jejuni subsp. jejuni and 46 campylobacter coli. the percentages of true results and 95% ci for disc diffusion for resistant strains were 100% (93.2-100%) for tetracycline (53 strains tested), 100% (77.2-100%) for ciprofloxacin (13 strains tested), 86.7% (62.1-96.3%) for nalidixic acid (15 strains tested), 100% (56.6-100%) for erythrom ...19979222038
indwelling device-related bacteremia caused by serum-susceptible campylobacter coli.two isolates of serum-susceptible campylobacter coli were recovered in a 7-day interval from blood from a patient with hepatocellular carcinoma and liver cirrhosis whose peritoneal-caval (denver's) shunt malfunctioned. identical random amplified polymorphic dna fingerprints, cellular fatty acid chromatograms, and antibiograms of the two isolates indicate that c. coli has the ability to cause catheter-related bacteremia following its colonization of the catheter.19979230411
molecular discrimination between campylobacter jejuni, campylobacter coli, campylobacter lari and campylobacter upsaliensis by polymerase chain reaction based on a novel putative gtpase gene.polymerase chain reaction (pcr) mediated dna fingerprinting has resulted in the identification of a novel campylobacter jejuni gene, encoding a gtpase protein. the gene, consisting of 383 amino acids contained semi-conserved gtp-binding sites (designated g-1 to g-4), that are characteristic for members of the gtpase protein superfamily. remarkably, this gene from c. jejuni appears to encode a member of a novel family of gtp-binding proteins, containing two separate putative gtp-binding domains, ...19979232616
differentiation of campylobacter jejuni and campylobacter coli by polymerase chain reaction.a multiplex pcr assay was developed using two primer sets for the identification and differentiation of campylobacter coli and campylobacter jejuni. primer set i amplifies a 460-bp fragment present in c. coli and c. jejuni. set ii amplifies a 160-bp target unique to c. jejuni. when the assay was performed on reference strains, amplification of c. coli yielded only the 460-bp fragment. amplification of c. jejuni generated both the 160- and 460-bp fragments. campylobacter field strains (n = 85) is ...19979232618
the flge gene of campylobacter coli is under the control of the alternative sigma factor sigma54.the flge gene encoding the flagellar hook protein of campylobacter coli vc167-t1 was cloned by immunoscreening of a genomic library constructed in lambdazap express. the flge dna sequence was 2,553 bp in length and encoded a protein with a deduced molecular mass of 90,639 da. the sequence had significant homology to the 5' and 3' sequences of the flge genes of helicobacter pylori, treponema phagedenis, and salmonella typhimurium. primer extension analysis indicated that the vc167 flge gene is co ...19979244248
expression of campylobacter hyoilei lipo-oligosaccharide (los) antigens in escherichia coli.campylobacter spp. are well recognized as primary pathogens in animals and in people. to isolate and define the genetic regions encoding major surface antigens of campylobacter hyoilei, genomic dna of the type strain of the species, rmit-32a, was cloned into a cosmid vector, pla2917, in escherichia coli and the resulting genomic library was screened using antiserum raised to the parent c. hyoilei strain. six cosmid clones were found to express a series of immunoreactive bands in the 15-25 kda ra ...19979387226
analysis of distribution of campylobacter jejuni and campylobacter coli in broilers by using restriction fragment length polymorphism of flagellin gene.the incidence of campylobacter jejuni and campylobacter coli in broiler farms was 33.9% (19/56). c. jejuni-positive flocks accounted for 20.0% (17/85) and c. coli-positive ones was 4.7% (4/85). there were 14 patterns (fla type) of restriction fragment length polymorphism (rflp) of flagellin a gene among these 22 strains of c. jejuni and c. coli including the standard strain c. jejuni atcc 33560. different fla types of campylobacter were isolated from broilers in different growing cycles on the s ...19979409516
[serodiagnosis of helicobacter pylori infections by detection of immunoglobulin g antibodies with an immunoblot technique].serological testing has recently been proposed as an aid in diagnosis of h. pylori infections. in this study, an immunoblot method has been evaluated to diagnose h. pylori infection serologically by comparing 104 serum specimens from patients with a positive gram stain and/or culture result and a positive urease test on biopsy material, as well as 30 serum specimens from patients with negative urease test, and negative microscopy and culture results. thirteen bands could be identified by immunob ...19969275637
[the sat4 streptothricin acetyltransferase gene of campylobacter coli: its distribution in the environment and use as epidemiological marker].the main of this study is to give evidence about the spread of streptothricin resistance within campylobacters which were isolated from animals, men and environmental sources. streptothricin resistant campylobacters were isolated over a five years period, when the use of streptothricin for ergotropic purposes was not allowed, from slurry of swine, slurry of cattle, waste water of a goose farm and waste water of a communal sewage treatment plant. the streptothricin resistance was found to be conn ...19969376053
enterotoxigenic capacity of campylobacter coli strains isolated from humans and bovines. 19969137747
ribotypes and ap-pcr fingerprints of thermophilic campylobacters from marine recreational waters.thirty-two strains of thermophilic campylobacters isolated from marine recreational water and seven reference strains were biotyped and analysed by chromosomal dna haeiii ribopatterns and ap-pcr profiles based on a random 10-mer primer (5'-caa tcg ccg t-3'). the majority of seawater isolates (90%) were campylobacter coli, and three strains were camp. jejuni. southern blot hybridization analysis showed differences between the strains, and in a numerical analysis three main clusters were formed at ...19968642012
identification of the tetracycline resistance gene, tet(o), in streptococcus pneumoniae.five isolates of streptococcus pneumoniae resistant to tetracycline but lacking tet(m) were studied. the tetracycline resistance gene, tet(o), was detected for the first time in the pneumococcus. the gene was amplified and sequenced and found to share 99% nucleotide sequence identity and 99, 99, and 98% deduced amino acid sequence identity with the tet(o) resistance genes of streptococcus mutans, campylobacter coli, and campylobacter jejuni, respectively.19969124862
rgfgigs is an amino acid sequence required for acetyl coenzyme a binding and activity of human spermidine/spermine n1acetyltransferase.polyamine catabolism is rate limited by spermidine/spermine n1-acetyltransferase (ssat). although the amino acid sequence of ssat is known, the substrate binding and catalytic sites are not. the goal of this study was to define the region responsible for acetyl coenzyme a binding. human ssat contains a region of 20 amino acids homologous to several microbial antibiotic n-acetyltransferases. the highest homology is represented in the campylobacter coli streptothricin acetyltransferase sat4 gene, ...19968702554
ciprofloxacin resistant campylobacter spp. in humans: an epidemiological and laboratory study.from the end of april 1991 until the end of 1991, 2209 isolates of campylobacter spp. have been collected in plymouth phl of which 91 (4.1%) were resistant to ciprofloxacin. none of the 91 patients involved had taken a quinolone, but 30/91 (33%) had travelled abroad (16 to the iberian peninsula) in the three months preceding isolation of the ciprofloxacin-resistant campylobacter spp. in the case-control study 12/15 (80%) of the cases had recently consumed poultry as had 20/24 (83%) of controls w ...19968722540
restriction fragment length polymorphism of flagellin genes of campylobacter jejuni and/or c. coli isolates from egypt.the conservation of flagellin genes from thermophilic campylobacter spp. strains isolated in egypt was evaluated by a restriction fragment length polymorphism (rflp) assay. the flaa and flab genes were amplified from 59 independent clinical isolates and digested with ecori and psti, and the resulting patterns were compared with each other and with previously described rflp groups. the results indicate that the isolates fell into 14 groups for flaa and 11 groups for flab, 9 of which have been des ...19968727905
soma, a novel gene that encodes a major outer-membrane protein of synechococcus sp. pcc 7942.the outer membrane of a cyanobacterium (synechococcus sp. strain pcc 7942) contains only a few major proteins. a gene encoding one of them, soma, was cloned and characterized. based on the nucleotide sequence, soma was predicted to comprise 531 amino acids with a calculated molecular mass of 57,136 da. the deduced amino acid sequence of soma shares similarities with two bacterial cell-surface proteins, the s-layer protein of thermus thermophilus and the flagellin of campylobacter coli. the predi ...19968760925
comprehensive ribotyping scheme for heat-stable serotypes of campylobacter jejuni.strains from diverse sources belonging to all 47 heat-stable penner serotypes of campylobacter jejuni were examined for polymorphism around the 16s rrna genes. penner serotype reference strains and a group of nonserotypeable isolates were included in the study. complete typeability was obtained; 30 distinct psti and 42 haeiii polymorphisms were found. three bands were detected in almost all strains with these enzymes, confirming that three copies of the 16s rrna gene are typical for c.jejuni. by ...19968788998
analysis of hl and o serotypes of campylobacter strains by the flagellin gene typing system.we recently developed a molecular typing system for campylobacter jejuni and campylobacter coli based on restriction fragment length polymorphism analysis of the flagellin gene,flaa (i.nachamkin, k. bohachick, and c.m. patton, j. clin. microbiol. 31:1531-1536, 1993). we extended the typing system to 83 flagellin types (designated flaa-1,flaa-2, etc.) on the basis of analysis of 404 isolates of c. jejuni and c. coli including common serotypes isolated in the united states, a selection of less com ...19968789000
an environmentally regulated pilus-like appendage involved in campylobacter pathogenesis.examination of strains of campylobacter jejuni, campylobacter coli, and campylobacter fetus by electron microscopy revealed that they produced peritrichous pilus-like appendages when the bacteria were grown in the presence of bile salts. various bile-salt supplements were used and it was found that deoxycholate and chenodeoxycholic acid caused a significant enhancement of pilus production and resulted in a highly aggregative phenotype. morphologically, the pili were between 4 and 7 nm in width a ...19968793884
identification and characterization of genes required for post-translational modification of campylobacter coli vc167 flagellin.two genes have been identified in campylobacter coli vc167 which are required for the biosynthesis of post-translational modifications on flagellin proteins. the ptma gene encodes a protein of predicted m(r) 28,486 which shows significant homology to a family of alcohol dehydrogenases from a variety of bacteria. the ptmb gene encodes a protein of predicted m(r) 26,598 with significant homology to cmp-n-acetylneuraminic acid synthetase enzymes involved in sialic acid capsular biosynthesis in neis ...19968825781
antimicrobial susceptibilities of campylobacter jejuni and campylobacter coli to 12 beta-lactam agents and combinations with beta-lactamase inhibitors.the in vitro activities of 12 beta-lactam agents against 100 thermophilic campylobacter strains were tested. beta-lactamase production was detected in 88% of all strains tested. clavulanic acid was the best inhibitor by susceptibility testing. the beta-lactams which displayed high levels of in vitro activity against campylobacter isolates were imipenem, amoxicillin-clavulanic acid, and cefepime and, to a lesser degree, amoxicillin, ampicillin, and cefotaxime.19968843305
hazard identification in swine slaughter with respect to foodborne bacteria.swine slaughter is an open process with many opportunities for the contamination of the pork carcass with potentially pathogenic bacteria; however, it does not contain any point where hazards are completely eliminated. data on the prevalence of various pathogenic bacteria (aeromonas hydrophila, campylobacter coli/jejuni, listeria monocytogenes, salmonella spp., staphylococcus aureus and yersinia enterocolitica) in pigs, their growth and survival characteristics and ability to become established ...19968856371
sub-typing of animal and human campylobacter spp. using rapd.based on a 10-mer primer (5'-cctgttagcc-3'), a random amplified polymorphic dna (rapd) method for typing campylobacter coli isolated from pigs was developed. the method proved effective with a high discrimination and good reproducibility. in contrast with serotyping no untypable strains were found out of a total of 269 isolates (veterinary, food and clinical) examined. the method was also successfully applied to typing campylobacter jejuni from a similar range of sources.19968862022
the lipooligosaccharides of pathogenic gram-negative bacteria.lipooligosaccharides (loss) are the major glycolipids expressed on mucosal gram-negative bacteria, including members of the genera neisseria, haemophilus, bordetella, and branhamella. they can also be expressed on some enteric bacteria such as campylobacter jejuni and campylobacter coli strains. los is analogous to the lipopolysaccharide (lps) found in other gram-negative families. loss share similar lipid a structures with an identical array of functional activities as lpss. loss lack o-antigen ...19968894399
development of a rapid and specific colony-lift immunoassay for detection and enumeration of campylobacter jejuni, c. coli, and c. lari.contamination of retail poultry by campylobacter spp. is a significant source of human diarrheal disease. we have developed a colony-lift immunoassay (cli) for the detection of campylobacter jejuni, c. coli, and c. lari isolated from such sources and grown on selective agar medium or on filter membranes. this technique has been successfully utilized to quantify campylobacter colonies within 18 to 28 h after sampling. hydrophobic, high-protein-binding membranes were prewet with methanol and used ...19968914757
a combined polymerase chain reaction and restriction endonuclease enzyme assay for discriminating between campylobacter coli and campylobacter jejuni.a combined polymerase chain reaction and restriction endonuclease (re) enzyme assay was developed to discriminate between campylobacter coli and campylobacter jejuni. amplimers of the flaa gene obtained by pcr were digested with alui and hinfi to distinguish c. coli from c. jejuni. with alui digestion c. jejuni-specific bands were observed at 110, 140 and 160 bp and c. coli-specific bands at 293 and 147 bp. c. jejuni-specific bands of 349 and 109 bp were found by hinfi digestion but hinfi did no ...19968954352
detection of campylobacter jejuni and camp. coli in chicken faecal samples by pcr.pcr primers were selected from the flagellin gene sequences flaa and flab of campylobacter coli to amplify dna from camp. jejuni and camp. coli. when the pcr products were analysed by hybridization to an internal probe immobilized in microtitre wells, positive reactions were observed only for strains of camp. jejuni and camp. coli. the assay was used to analyse 31 chicken faecal samples. full correspondence was found between the pcr assay conducted on the enriched cultures and the standard cultu ...19968987720
polymerase chain reaction assay for detection of campylobacter coli and campylobacter jejuni in poultry meat.primers of 16s rrna and flaa genes were used to optimise a pcr technique for detecting thermotolerant campylobacters in poultry meat. different methods for crude dna extraction were also evaluated. the use of flaa primers and extraction of nucleic acid by boiling and proteinase k gave good results in the detection of campylobacter either in artificially or naturally contaminated foodstuffs. the lowest sensitivity limit for the pcr reaction was 10(1)-10(2) thermophilic campylobacter cells either ...19958672221
campylobacter hyoilei sp. nov., associated with porcine proliferative enteritis.campylobacter hyoilei sp. nov. is the name proposed for an organism formerly described as strain rmit 32at (t = type strain) and a group of similar bacteria isolated from intestinal lesions of pigs with proliferative enteritis. the phenotypic characteristics of these organisms indicated that they are closely related to each other and are not strains of other campylobacter spp. commonly isolated from pigs. the results of probing of clai-, ecorv-, or bglii-cleaved genomic dnas from c. hyoilei stra ...19957857809
expression and characterization of campylobacter jejuni benzoylglycine amidohydrolase (hippuricase) gene in escherichia coli.the basis for the difference between campylobacter jejuni and campylobacter coli is the presence and expression of the n-benzoylglycine amidohydrolase (hippuricase) gene only in c. jejuni. a pbr322 recombinant clone (phip-o) of c. jejuni tgh9011 capable of converting hippuric acid into benzoic acid and glycine, the hallmark of hippuricase activity, was characterized and sequenced. the hippuricase gene (hipo) was identified by use of deletion subclones and insertional inactivation. the transcript ...19957730270
prenatal diagnosis of microbial invasion of the amniotic cavity with campylobacter coli in preterm labour. 19957833319
quinolone resistance in clinical strains of campylobacter jejuni and campylobacter coli. 19958537280
the speciation and subtyping of campylobacter isolates from sewage plants and waste water from a connected poultry abattoir using molecular techniques.in this study the distribution of phenotypes of campylobacter strains in sewage and surface waters was investigated by subtyping and by speciation of isolates from various aquatic environments. these environments included two municipal sewage plants (spa and spb) and waste water from a poultry abattoir (wwa). both the sewage plants spa and spb collected domestic and industrial waste, and spa received drain water from wwa. spb received no waste water from any meat-processing plant. the isolates w ...19958557080
enterochelin acquisition in campylobacter coli: characterization of components of a binding-protein-dependent transport system.siderophore-mediated iron uptake systems play a central role in the pathogenesis of infection for many bacterial pathogens. campylobacter species are not thought to produce siderophores, yet they are able to utilize both ferrichrome and enterochelin as sources of iron. part of an operon named ceubcde, encoding components of a periplasmic binding-protein-dependent transport (pbt) system for the uptake of a ferric siderophore from campylobacter coli, was cloned directly into escherichia coli using ...19958574410
characterization of the type strain of campylobacter coli, cip 70.80, by plasmid typing.a 1.9-kb plasmid dna fragment from the type strain of campylobacter coli, cip 70.80, was used as a probe to characterize this type strain, other c. coli type strains obtained from several culture collections, and other c. coli strains. a specific hybridization pattern was obtained, and this pattern can be used to identify, characterize, and follow up c. coli type strains in culture collections.19958590689
[the comparative identification of campylobacter strains by traditional enzymatic tests and the gene amplification reaction].39 strains of campylobacter isolated from 153 diarrhoeal children (0-3 years) were comparatively identified by the traditional enzymatic tests and by the polymerase chain reaction (pcr). the hippurate hydrolysis test appreciated 27 strains as campylobacter jejuni (69.2%) and 12 strains as campylobacter coli (30.8%). the p.c.r. realised in france has appreciated 29 strains as campylobacter jejuni (74.4%) and 10 strains as campylobacter coli (25.6%). the analysis of the results discrepancy permitt ...19958640005
invasive and enterotoxic properties in campylobacter jejuni and campylobacter coli strains isolated from humans and animals.invasive properties of 15 strains of thermotolerant campylobacter (12 c. jejuni and 3 c. coli) were studied using hela cells cultures. in four of them (3 c. jejuni and 1 c. coli), randomly selected, intestinal perfusion experiments were conducted in order to asses enterotoxigenicity. all strains were able to invade hela cells. the number of invaded hela cells ranged from 3 to 46%. in addition to their invasive properties, the 4 strains used in perfusion experiments were able to induce either a n ...19959251750
enrichment medium for isolation of campylobacter jejuni-campylobacter coli.a kaseini-broth enrichment medium (kbem) for the isolation improvement of campylobacter jejuni-campylobacter coli from stool samples is described. isolation of campylobacters from stool specimens by direct inoculation onto solid selective medium was compared with isolation after twenty-four hours enrichment at 37 degrees c in kbem, followed by subculture onto the same solid selective medium. of 156 examined stool samples from diarrhoeal children positive results were obtained from 17 patients al ...19959524671
detection and identification of campylobacter coli and campylobacter jejuni by two-step polymerase chain reaction.flagellin gene was used as target sequence to detect and distinguish c. coli and c. jejuni by a "nested pcr" technique. the method shows a high level of sensitivity and specificity. application of this rapid diagnostic tool could provide further information about epidemiological and pathogenetic implications of each of these two microorganisms.19957552696
high-resolution genotyping of campylobacter coli identifies clones of epidemiologic and evolutionary significance.campylobacter coli strains from clinical and other sources were examined in terms of o (heat-stabile; hs) serotype and by several molecular typing techniques. restriction fragment length polymorphism (rflp) around the three 16s rrna genes revealed 10 variants, none found in campylobacter jejuni. rflp analysis of a polymerase chain reaction amplicon generated from the flagellin gene (flaa) yielded 11 polymorphism groups, some of them linked to hs serotypes. enlarged flaa genes, contributing three ...19957561196
effect of the complement system on the sensitivity of campylobacter jejuni and campylobacter coli to human blood serum.we determined the sensitivity of five strains of campylobacter jejuni and c. coli isolated from children with diarrhea and from chicken feces to normal human blood serum (undiluted and at concentrations of 10, 30, 50 and 70%), a hypogammaglobulinemic serum and a complement-deficient serum. both species were highly sensitive to the bactericidal activity of human serum, regardless of their source. the highest bactericidal activity was observed with pooled fresh normal serum, with bacterial surviva ...19957581046
comparison of two selective media and a membrane filter technique for isolation of campylobacter species from diarrhoeal stools.diarrhoeal stool specimens from 415 patients were examined for campylobacter spp. by culture on charcoal cefoperazone deoxycholate agar (ccda), skirrow medium and columbia blood agar overlaid with a 0.65 micron pore size membrane filter. forty-eight campylobacter strains were isolated from 45 (10.8%) specimens by all media; 44 were campylobacter jejuni (91.7%), three were campylobacter coli (6.3%) and one was campylobacter hyointestinalis (2.0%). the percentages of campylobacter-positive specime ...19957588831
targeted and random mutagenesis of the campylobacter coli chromosome with integrational plasmid vectors.a number of integrational vectors were developed for use as genetic tools in the food-borne pathogen campylobacter coli. integration of the plasmids occurred following genetic recombination via a campbell-like mechanism. for an integrative plasmid containing a dna fragment internal to the c. coli catalase gene, the insertion was mutagenic and led to a catalase-deficient phenotype. a procedure for generating random mutations in the c. coli chromosome, with these suicide-plasmids, was developed. i ...19957606191
differentiation of campylobacter jejuni and campylobacter coli strains by using restriction endonuclease dna profiles and dna fragment polymorphisms.the chromosomal dna fragment patterns from a total of 169 campylobacter jejuni and campylobacter coli isolates from poultry and humans were analyzed by using dna restriction endonucleases clai and ecorv. the dna restriction patterns produced by clai and ecorv consisted of unique dna fragments of 9 to 9.5 kb and 3.5 kb generated with clai and a single unique fragment of 3.0 kb produced by ecorv. these patterns were obtained with all strains of c. jejuni tested. the dna restriction patterns were f ...19957615717
[infectivity of campylobacter jejuni and campylobacter coli in chickens].the infectivity of four campylobacter (c.) jejuni strains and one c. coli strain was evaluated by the reisolation rate from cloacal swabs after oral infection of chickens at four weeks of age. one strain did not colonize the digestive tract, another infected only a part of the experimental group and a third was excreted at a lower percentage in the first two weeks p.i. compared to the other two strains. in correlation to age, the excretion rate was highest in 7-week-old birds. in groups of 4 and ...19957646423
rapid detection of campylobacter jejuni and campylobacter coli isolated from clinical specimens using the polymerase chain reaction.seventeen campylobacter strains isolated from 16 children hospitalised with acute diarrhea were analysed by in vitro enzymatic amplification using two sets of oligonucleotide primers specific for campylobacter jejuni and campylobacter coli, respectively. thirteen strains (76%) were identified as campylobacter jejuni and four strains (24%) as campylobacter coli. subsequent bacteriological identification confirmed the identity of the same 13 campylobacter jejuni strains and the 4 campylobacter col ...19957649203
anti-gm1 igg antibodies and campylobacter bacteria in guillain-barré syndrome: evidence of molecular mimicry.in guillain-barré syndrome antibodies to gm1 and the presence of an antecedent campylobacter jejuni infection are correlated with a more severe course of the disease. from a group of 137 consecutive gbs patients, 11 sera had elevated titers of anti-gm1 igg antibodies during the acute stage of disease. each serum sample was preincubated with three different penner serotypes of whole c. jejuni (pen o:4/59, pen o:41) and campylobacter coli (pen o:22) bacteria. the pen o:4/59 serotype, isolated from ...19957654064
a survey of campylobacter bacteremia in three danish counties, 1989 to 1994.over a 5-year period (april 1989 to march 1994) bacteremia caused by campylobacter species was diagnosed in 15 patients in a population of 1.3 million in three counties in jutland province, denmark. the incidence was 0.2/100,000/year and the ratio of blood to fecal isolates, was 0.008. campylobacter coli and campylobacter jejuni accounted for 6 cases each, campylobacter fetus for 2, and in 1 case the species diagnosis was uncertain. seven patients were males and 8 females; median age was 42 (ran ...19957660078
molecular characterization of kata from campylobacter jejuni and generation of a catalase-deficient mutant of campylobacter coli by interspecific allelic exchange.a gene encoding catalase (hydrogen-peroxide:hydrogen-peroxide oxidoreductase; ec 1.11.1.6) from campylobacter jejuni was cloned by functional complementation of a catalase-deficient mutant of escherichia coli. the catalase structural gene, designated kata, was assigned by subcloning and its nucleotide sequence determined. the deduced protein product of 508 amino acids, which had a calculated molecular mass of 58,346 da, was found to be structurally and enzymically similar to hydrogen-peroxidases ...19957670638
production of monoclonal antibody specific to campylobacter jejuni and its potential in diagnosis of campylobacter enteritis.a monoclonal antibody (mab 3g6) specific for campylobacter jejuni and campylobacter coli was produced by immunizing balb/c mice with a local strain of c.jejuni (28.1). no cross-reactivity was observed with enterobacteriaceae controls. by immunoprecipitation, mab 3g6 identified a major protein band of molecular weight 45 kda and also gave a slight reactivity with 30 and 55 kda proteins. using an indirect enzyme-linked immunosorbent assay, mab 3g6 was able to detect c.jejuni suspended in stool wit ...19957488346
Displaying items 1901 - 2000 of 2341