TitleAbstractYear(sorted ascending)
intersubspecific recombination in xylella fastidiosa strains native to the united states: infection of novel hosts associated with an unsuccessful invasion.the bacterial pathogen xylella fastidiosa infects xylem and causes disease in many plant species in the americas. different subspecies of this bacterium and different genotypes within subspecies infect different plant hosts, but the genetics of host adaptation are unknown. here we examined the hypothesis that the introduction of novel genetic variation via intersubspecific homologous recombination (ihr) facilitates host shifts. we investigated ihr in 33 x. fastidiosa subsp. multiplex isolates pr ...201424296499
biological roles of the o-methyl phosphoramidate capsule modification in campylobacter jejuni.campylobacter jejuni is a major cause of bacterial gastroenteritis worldwide, and the capsular polysaccharide (cps) of this organism is required for persistence and disease. c. jejuni produces over 47 different capsular structures, including a unique o-methyl phosphoramidate (meopn) modification present on most c. jejuni isolates. although the meopn structure is rare in nature it has structural similarity to some synthetic pesticides. in this study, we have demonstrated, by whole genome comparis ...201424498018
presence and analysis of plasmids in human and animal associated arcobacter this study, we report the screening of four arcobacter species for the presence of small and large plasmids. plasmids were present in 9.9% of the 273 examined strains. one arcobacter cryaerophilus and four arcobacter butzleri plasmids were selected for further sequencing. the size of three small plasmids isolated from a. butzleri and the one from a. cryaerophilus strains ranged between 4.8 and 5.1 kb, and the size of the large plasmid, isolated from a. butzleri, was 27.4 kbp. the g+c content ...201424465575
detection of cdt toxin genes in campylobacter spp. strains isolated from broiler carcasses and vegetables in são paulo, brazil.campylobacteriosis is a worldwide distributed zoonosis. one of the main virulence factors related to campylobacter spp. in animals and humans is the cytolethal distending toxin (cdt), encoded by three adjacent genes (cdta, cdtb, cdtc). the occurrence of campylobacter spp. in samples of vegetables has not been reported in brazil yet, and has seldom been described in the international literature. the detection of cdt in these strains has not been reported, either. the objectives of the present stu ...201424516435
isolation and antimicrobial susceptibility patterns of campylobacter species among diarrheic children at jimma, ethiopia.introduction. campylobacter is one of the leading bacterial causes of food-borne disease. the prevalence of campylobacter species resistant to antimicrobial agents is increasing. this study is intended to determine prevalence and antimicrobial susceptibility patterns of campylobacter species among under-five children with diarrhea. methodology. a cross-sectional study was conducted among 227 under-five children with diarrhea from july to october 2012 at jimma town. isolation and identification o ...201426904735
the renaissance of bacillosamine and its derivatives: pathway characterization and implications in pathogenicity.prokaryote-specific sugars, including n,n'-diacetylbacillosamine (dinacbac) and pseudaminic acid, have experienced a renaissance in the past decade because of their discovery in glycans related to microbial pathogenicity. dinacbac is found at the reducing end of oligosaccharides of n- and o-linked bacterial protein glycosylation pathways of gram-negative pathogens, including campylobacter jejuni and neisseria gonorrhoeae. further derivatization of dinacbac results in the nonulosonic acid known a ...201424383882
comparison of characteristics of patients infected by campylobacter jejuni, campylobacter coli, and campylobacter fetus.a large database of campylobacter isolates precisely identified at the species level was used to compare patients' characteristics. in a multivariate analysis, campylobacter coli was found more often in older patients and in patients having traveled abroad and less often in summertime than campylobacter jejuni. campylobacter fetus infection occurred in much older patients and in hospitalized patients with a systemic disease.201424197884
lipooligosaccharide locus class of campylobacter jejuni: sialylation is not needed for invasive infection.campylobacter jejuni is a highly diverse enteropathogen that is commonly detected worldwide. it can sometimes cause bacteraemia, but the bacterial characteristics facilitating bloodstream infection are not known. a total of 73 c. jejuni isolates, consecutively collected from blood-borne infections during a 10-year period all over finland and for which detailed clinical information of the patients were available, were included. we screened the isolates by pcr for the lipooligosaccharide (los) loc ...201424102802
target optimization for peptide nucleic acid (pna)-mediated antisense inhibition of the cmeabc multidrug efflux pump in campylobacter jejuni.cmeabc is a resistance-nodulation-cell division (rnd)-type multidrug efflux pump conferring resistance to clinically important antibiotics in campylobacter. this study aimed to identify the optimal target sites for the inhibition of cmeabc with antisense peptide nucleic acid (pna).201424084637
an epidemiological investigation of campylobacter in pig and poultry farms in the mekong delta of vietnam.campylobacter are zoonotic pathogens commonly associated with gastroenteritis. to assess the relevance of campylobacter in vietnam, an economically transitioning country in se asia, we conducted a survey of 343 pig and poultry farms in the mekong delta, a region characterized by mixed species farming with limited biosecurity. the animal-level prevalence of campylobacter was 31·9%, 23·9% and 53·7% for chickens, ducks and pigs, respectively. c. jejuni was predominant in all three host species, wit ...201424067502
identification and characterization of an invasion antigen b gene from the oral pathogen campylobacter rectus.the oral bacterium, campylobacter rectus, is an etiological agent of periodontitis. the virulence genes of c. rectus are largely unknown. the aim of this study was to query c. rectus for the presence of an invasion antigen b (ciab) gene, which is needed for cell invasion by the related species campylobacter jejuni. pcr and pcr-walking identified a ciab from c. rectus. in silico analyses of c. rectus 314 ciab (cr-ciab) revealed an orf of 1,830 base pairs. the cr-ciab protein shared significant se ...201424426164
genotypes and antibiotic resistance of canine campylobacter jejuni isolates.campylobacter jejuni is the most important cause of bacterial gastroenteritis in humans. it is a commensal in many wild and domestic animals, including dogs. whereas genotypes of human and chicken c. jejuni isolates have been described in some detail, only little information on canine c. jejuni genotypes is available. to gain more information on genotypes of canine c. jejuni and their zoonotic potential, isolates from routine diagnostics of diarrheic dogs as well as isolates of a prevalence stud ...201424210812
prevalence of three campylobacter species, c. jejuni, c. coli, and c. lari, using multilocus sequence typing in wild birds of the mid-atlantic region, usa.campylobacter jejuni is responsible for the majority of bacterial foodborne gastroenteritis in the us, usually due to the consumption of undercooked poultry. research on which avian species transmit the bacterium is limited, especially in the us. we sampled wild birds in three families-anatidae, scolopacidae, and laridae-in eastern north america to determine the prevalence and specific strains of campylobacter. the overall prevalence of campylobacter spp. was 9.2% for all wild birds sampled (n = ...201424171567
[spontaneous bacteremia due to campylobacter coli in a cirrhotic patient]. 201424280064
multilocus sequence typing of campylobacter jejuni and campylobacter coli from humans and chickens in north-eastern italy.this paper reports the multilocus sequence typing (mlst) of 57 c. jejuni and c. coli isolates from humans and chickens in italy and the identification of 17 new sequence types (sts). a high genetic diversity was detected among c. jejuni/c. coli and human/chicken isolates, with a predominance of clonal complexes cc21 and cc828. although human sts were not the same as those found in chickens, 3 ccs overlapped between human and chicken isolates. genotyping of campylobacter strains by mlst should be ...201425387294
prevalence of salmonella and campylobacter on broiler chickens from farm to slaughter and efficiency of methods to remove visible fecal contamination.a study was conducted to investigate the prevalence of salmonella and campylobacter from farm to slaughter. the efficiency of trimming and water spray (490 to 588 kpa pressure) on the removal of visible fecal contamination from broiler carcasses before chilling was also investigated. drag swabs were used to sample litter from the farm houses. samples of ceca and carcasses without and with visible fecal contamination before and after trimming or spray washing of fecal contamination were taken dur ...201425364917
[enumeration and antimicrobial resistance of campylobacter species from retail chicken carcasses].to determine campylobacter contamination level and antimicrobial resistance patterns from chicken carcasses in supermarkets and farmer's markets of 9 districts in beijing.201425573127
seasonality and antibiotic resistance of campylobacter in turkish chicken meat.this study investigated the seasonal prevalence and the antimicrobial susceptibility of campylobacter jejuni and campylobacter coli in 264 samples of chicken meat. the samples encompassed wings (n=88), breasts (n=79) and thighs (n=97) and were purchased from different butcheries and markets in elazig province, in eastern turkey, between december 2009 and november 2010. the meat samples were tested for campylobacter presence and the collected isolates were identified as campylobacter jejuni and c ...201425546065
interaction between campylobacter and intestinal epithelial cells leads to a different proinflammatory response in human and porcine host.campylobacter jejuni and campylobacter coli are recognized as the leading causes of human diarrheal disease throughout the development world. unlike human beings, gastrointestinal tract of pigs are frequently colonized by campylobacter to a high level in a commensal manner. the aim of this study was to identify the differences underlying the divergent outcome following campylobacter challenge in porcine versus human host. in order to address this, a comparative in vitro infection model was combi ...201425307769
method-dependent variability in determination of prevalence of campylobacter jejuni and campylobacter coli in canadian retail poultry.campylobacter is the most frequent cause of bacterial gastroenteritis in canada, and the illness is commonly associated with poultry consumption. whereas canadian retail poultry is often contaminated with campylobacters, studies on the prevalence of this organism are inconsistent due to variability in sampling and microbiological methodology. to determine the current microbiological status of canadian poultry, and to evaluate two commonly used microbiological methods, 348 raw poultry samples wer ...201425285484
sensitive and rapid detection of campylobacter species from stools of children with diarrhea in japan by the loop-mediated isothermal amplification method.we detected campylobacter spp. in 5% (20/380) of diarrheal stool samples collected at an outpatient clinic in kyoto using a commercial loop-mediated isothermal amplification (lamp) kit with a fluorescent detection reagent after dna extraction. the sensitivity and specificity were 100% in comparison with those of semi-nested pcr for the differentiation of campylobacter jejuni and campylobacter coli. fourteen of the 20 samples were already determined as c. jejuni by the culture method. all 20 samp ...201425241688
isolation of campylobacter from brazilian broiler flocks using different culturing procedures.conventional culturing methods enable the detection of campylobacter in broiler flocks. however, laboratory culture of campylobacter is laborious because of its fastidious behavior and the presence of competing nontarget bacteria. this study evaluated different protocols to isolate campylobacter from broiler litter, feces, and cloacal and drag swabs. samples taken from commercial brazilian broiler flocks were directly streaked onto preston agar (pa), campy-line agar (cla), and modified charcoal ...201425172927
prevalence of thermotolerant campylobacter spp. in farmed hares (lepus europaeus).thermotolerant campylobacter spp. were isolated from 118/240 (49.2%) rectal swabs from commercially farmed hares (lepus europaeus) in southern italy. using multiplex pcr, campylobacter coli was identified in 118/118 (100%) positive samples, while 17/118 (14.4%) positive samples were also positive for campylobacter jejuni. adult hares had a higher prevalence of infection with campylobacter spp. than juvenile hares.201425168717
identification and characterisation of new campylobacter group iii phages of animal origin.campylobacter-specific bacteriophages (phages) are considered as an alternative intervention strategy to decrease the level of poultry contamination with campylobacter, a leading cause of gastroenteritis worldwide. eradication efficiency depends primarily on phage-host interaction mediated by phage tail-spike proteins and bacterial receptors. here, this interaction was characterised using tail-spike gene sequence analysis, phage neutralisation by antiserum and host range analysis of newly isolat ...201425168177
experimental study of the impact of antimicrobial treatments on campylobacter, enterococcus and pcr-capillary electrophoresis single-strand conformation polymorphism profiles of the gut microbiota of experiment was conducted to compare the impact of antimicrobial treatments on the susceptibility of campylobacter, enterococcus faecium and enterococcus faecalis, and on the diversity of broiler microbiota. specific-pathogen-free chickens were first orally inoculated with strains of campylobacter and enterococcus faecium. birds were then orally treated with recommended doses of oxytetracycline, sulfadimethoxine/trimethoprim, amoxicillin or enrofloxacin. faecal samples were collected before, d ...201425142966
a pre-enrichment step is essential for detection of campylobacter sp. in turbid pond water.this work aimed to detect campylobacter species from naturally contaminated turbid pond water by pcr. a total of 16 water samples were collected from a turbid village pond. four methods of dna extraction were applied to centrifuge pellets from eight 100 ml pond water samples prior to attempted detection of campylobacter by pcr without an enrichment step. these methods were (1) tris-hcl and sodium dodecyl sulfate followed by phenol:chloroform:isoamylalcohol extraction followed by treatment with d ...201425134901
human, food and animal campylobacter spp. isolated in portugal: high genetic diversity and antibiotic resistance rates.infections by campylobacter jejuni and campylobacter coli are considered the major cause of bacterial gastroenteritis in humans, with food being the main source of infection. in this study, a total of 196 campylobacter strains (125 isolates from humans, 39 from retail food and 32 from food animal sources) isolated in portugal between 2009 and 2012 were characterised by multilocus sequence typing (mlst) and flaa short variable region (svr) typing. susceptibility to six antibiotics as well as the ...201425130097
quantitative effects of in-line operations on campylobacter and escherichia coli through two australian broiler processing plants.campylobacter is an important food borne pathogen, mainly associated with poultry. a lack of through-chain quantitative campylobacter data has been highlighted within quantitative risk assessments. the aim of this study was to quantitatively and qualitatively measure campylobacter and escherichia coli concentration on chicken carcasses through poultry slaughter. chickens (n=240) were sampled from each of four flocks along the processing chain, before scald, after scald, before chill, after chill ...201425104301
campylobacter spp. and birds of prey.a total of 170 birds of prey admitted to two wildlife rescue and rehabilitation centers of italy were examined. birds were divided by diurnal (n = 15) and nocturnal (n = 7) species, sampled by cloacal swabs, and examined for campylobacter spp. by cultural and molecular methods. campylobacter spp. were isolated in 43 out of the 170 (25.3%) birds of prey examined. among these, 43/43 (100%) were identified as campylobacter jejuni and 10/43 (23.3%) were identified as campylobacter coli recovered fro ...201425055637
development and assessment of molecular diagnostic tests for 15 enteropathogens causing childhood diarrhoea: a multicentre study.childhood diarrhoea can be caused by many pathogens that are difficult to assay in the laboratory. molecular diagnostic techniques provide a uniform method to detect and quantify candidate enteropathogens. we aimed to develop and assess molecular tests for identification of enteropathogens and their association with disease.201425022434
prevalence of and risk factors for campylobacter colonisation in broiler flocks at the end of the rearing period in france.1. a study was conducted to estimate the prevalence and quantification by species of campylobacter infection in broiler flocks at the end of the rearing period and to identify associated risk factors. 2. a questionnaire about the rearing period was completed and caecal samples were collected from 121 broiler flocks in brittany, france, during 2008. 3. campylobacter was isolated in 87 out of 121 flocks--a prevalence of 71.9% (95% ci, 63.7-80.1%), including 40.5% of campylobacter jejuni and 29.8% ...201425010255
antibiotic susceptibility pattern and genotyping of campylobacter species isolated from children suffering from study the prevalence and the antimicrobial resistance of campylobacter species isolated from children suffering from gastroenteritis .201425008814
a national investigation of the prevalence and diversity of thermophilic campylobacter species in agricultural watersheds in canada.the occurrence and diversity of thermophilic campylobacter species (c. jejuni, coli, and lari) were studied in water samples from four river basins located across canada. these basins located in quebec (bras d'henri), alberta (oldman), ontario (south nation), and british columbia (sumas) represented some of the most intensive farming areas in canada for hog, beef cattle, dairy cattle, and poultry, respectively. this study analyzed 769 water samples collected from 23 monitoring sites with agricul ...201424930011
rapid detection and differentiation of important campylobacter spp. in poultry samples by dot blot and pcr.the detection of campylobacter, the most commonly reported cause of foodborne gastroenteritis in the european union, is very important for human health. the most commonly recognised risk factor for infection is the handling and/or consumption of undercooked poultry meat. the methods typically applied to evaluate the presence/absence of campylobacter in food samples are direct plating and/or enrichment culture based on the horizontal method for detection and enumeration of campylobacter spp. (iso ...201424929879
antimicrobial resistance among campylobacter spp. strains isolated from different poultry production systems at slaughterhouse level.the aim of the current work was to evaluate the prevalence and antimicrobial susceptibility of campylobacter spp. isolated from different chicken production systems at the slaughterhouse level. chicken sampling at slaughterhouse was performed for cecum, carcass, and breast meat from flocks of organic (n = 6), extensive indoor (n = 14), and intensive production (n = 14), totaling 34 ceca pools, 64 neck skin pools, and 132 breasts, representing 96,386 chickens. a collection of 167 strains were ide ...201424879708
molecular subtyping and antimicrobial susceptibilities of campylobacter coli isolates from diarrheal patients and food-producing animals in china.the aim of this study was to determine the molecular subtyping and antimicrobial susceptibility characteristics of campylobacter coli isolates from different sources in china. one hundred thirteen c. coli isolates were subtyped by pulsed-field gel electrophoresis (pfge), multilocus sequence typing (mlst), and pora and flaa short variable region (svr) nucleotide sequences. cluster analysis was performed based on the pfge and sequence types (st). eighty-four pfge patterns (smai) were observed in 1 ...201424844559
supplementation of bolton broth with triclosan improves detection of campylobacter jejuni and campylobacter coli in chicken carcass rinse.we compared bolton enrichment broth supplemented with antimicrobial triclosan (t-bolton broth) and normal bolton broth for the isolation of campylobacter jejuni (c. jejuni) and campylobacter coli (c. coli) from chicken carcass rinse. whole chickens were rinsed with buffered peptone water prior to enrichment in normal bolton broth or t-bolton broth, followed by inoculation onto modified charcoal-cefoperazone-deoxycholate agar (mccda). suspect colonies were confirmed by pcr. we observed a signific ...201424813626
loads and antimicrobial resistance of campylobacter spp. on fresh chicken meat in nueva ecija, philippines.this study was performed to determine the prevalence and to semiquantify campylobacter spp. on chicken meat samples at 4 selected local wet markets in nueva ecija, philippines, and to determine the antimicrobial resistance patterns of the campylobacter isolates. out of 120 chicken meat samples, 57 (47.5%) were campylobacter spp. positive. the majority of isolated campylobacter strains were identified as campylobacter coli (54.4%) and 45.6% as campylobacter jejuni. most of these positive samples ...201424795322
marinade with thyme and orange oils reduces salmonella enteritidis and campylobacter coli on inoculated broiler breast fillets and whole wings.essential oils have been reported to possess antimicrobial properties and therefore have potential usage as natural antimicrobials in food. in a previous study, thyme orange essential oil combination (toc) used at the 0.5% level as a dip application on chicken cut-up parts had a significant antibacterial effect against salmonella and campylobacter. a study was designed to evaluate the effect of salt-phosphate marinade solution containing 0.5% toc to 1) reduce salmonella enteritidis and campyloba ...201424795320
outbreak of campylobacteriosis associated with a long-distance obstacle adventure race--nevada, october 2012.on october 12, 2012, the nellis air force base public health flight (nellis public health), near las vegas, nevada, was notified by the mike o'callaghan federal medical center (mofmc) emergency department (ed) of three active-duty military patients who went to the ed during october 10-12 with fever, vomiting, and hemorrhagic diarrhea. initial interviews by clinical staff members indicated that all three patients had participated october 6-7 in a long-distance obstacle adventure race on a cattle ...201424785983
molecular epidemiology and antimicrobial susceptibility of campylobacter coli clinical isolates.campylobacter spp. is a major cause of acute bacterial diarrhea in humans worldwide, and c. coli is responsible for 10% of the cases.201424768104
prevalence of zoonotic campylobacter spp. in rectal swabs from dogs in slovakia: special reference to c. jejuni and c. coli.this work focused on the isolation of thermophilic campylobacter spp. in samples obtained from dog droppings. there were 135 samples collected and examined from both clinically healthy and diseased dogs from households, clinics, rehabilitation centres and dog shelters in eastern slovakia. the isolation of the campylobacter spp. was achieved by the use of combined selective cultivation methods, followed by confirmation and species identification of the isolates using the pcr method.the overall pr ...201424693660
molecular mechanisms of quinolone, macrolide, and tetracycline resistance among campylobacter isolates from initial stages of broiler production.the aim of this study was to investigate the resistance mechanisms of quinolones, macrolides and tetracycline in campylobacter isolates from grandparent and parent broiler breeders in spain. twenty-six isolates were investigated for quinolone resistance, three isolates for macrolide resistance and 39 for tetracycline resistance. all of the quinolone-resistant isolates possessed the mutation thr86ile in the quinolone resistance-determining region of gyra and one isolate possessed the mutation pro ...201424689432
evaluation and single-laboratory verification of a proposed modification to the u.s. food and drug administration method for detection and identification of campylobacter jejuni or campylobacter coli from raw silo milk.the current u.s. food and drug administration (fda) methodology for detection of campylobacter, a leading source for foodborne illness, is outdated. the purpose of this study, therefore, was to improve and update the cultural and identification methods found in the fda/bacteriological analytical manual (bam). raw silo milk samples containing typical and atypical strains of campylobacter jejuni and campylobacter coli at different levels (5 cfu/25 g, 50 cfu/25 g, and 125 cfu/25 g) were analyzed. v ...201424645512
validation according to iso 16140:2003 of a commercial real-time pcr-based method for detecting campylobacter jejuni, c. coli, and c. lari in foods.campylobacteriosis was the most frequently reported zoonosis in the european union (eu) in 2010, with campylobacter jejuni, campylobacter coli, and campylobacter lari as the most frequently reported species in foodborne outbreaks (fbos). relatively sensitive to environmental factors, these species may be present in low numbers. in line with eu policy for food control and fbo detection and in view of the need to reduce response time, we validated an alternative molecular method according to iso 1 ...201424607425
a pcr-rflp assay for the detection and differentiation of campylobacter jejuni, c. coli, c. fetus, c. hyointestinalis, c. lari, c. helveticus and c. upsaliensis.although campylobacter jejuni and campylobacter coli are the most common bacterial causes of human gastrointestinal diseases, other campylobacter species are also involved in human and animal infections. in this study, we developed a cytolethal distending toxin (cdt) gene-based pcr-rflp assay for the detection and differentiation of c. jejuni, c. coli, c. fetus, c. hyointestinalis, c. lari, c. helveticus and c. upsaliensis. previously designed common primers, which can amplify the cdtb gene of c ...201424568882
prevalence of campylobacter among goats and retail goat meat in congo.the prevalence of campylobacter jejuni and campylobacter coli was determined in goat and goat meat sold at retail outlets in lubumbashi, democratic republic of congo (dr congo).201424518626
[microbiological diagnosis of infections caused by campylobacter jejuni and campylobacter coli in humans].campylobacter jejuni and campylobacter coli are gram-negative, microaerophilic bacteria which are worldwide in distribution, causing a zoonotic disease in humans called campylobacteriosis. these infections are mainly caused by eating contaminated food products, most often improperly prepared poultry meat. campylobacteriosis usually takes the form of gastroenteritis, or inflammation of the intestines, and the characteristic symptoms are watery-mucous diarrhea often with the presence of blood in s ...201424491895
campylobacter coli cultured from the stools of a patient with immunoproliferative small intestinal disease.campylobacter has been associated with immunoproliferative small intestinal disease (ipsid), on the basis of 16s rdna sequencing, in situ hybridization, and immunohistochemistry. here, for the first time, we have cultured campylobacter from the stools of a patient with ipsid. phenotypic analysis and whole genome sequencing identified campylobacter coli. pcr on a ipsid tissue biopsy sample was positive for campylobacter coli and negative for campylobacter jejuni. these findings further support a ...201424438451
diversity of campylobacter in retail meat and liver of lambs and goat kids.the presence, genetic diversity, and antimicrobial susceptibility profile of campylobacter spp. in retail lamb and goat kid carcasses were assessed. a total of 200 samples consisting of 100 meat and 100 liver surface swabs were collected from 47 lamb and 53 goat kid carcasses at 23 retail markets in northern greece, and 125 campylobacter isolates were recovered from 32 meat surfaces (32%) and 44 liver surfaces (44%). multiplex polymerase chain reaction and restriction fragment length polymorphis ...201424437705
campylobacter in small ruminants at slaughter: prevalence, pulsotypes and antibiotic resistance.the present study aimed to address the prevalence, pulsotypes, and antimicrobial susceptibility patterns of campylobacter species present in sheep and goat carcasses at slaughter. in total, 851 samples were collected (343 meat surfaces, 282 ileum contents, 226 liver surfaces) and 835 campylobacter isolates were detected in 274 out of 343 carcasses (116 kids, 110 lambs, 63 goats and 54 sheep). the contamination rates per carcass category were 78.4% for kids, 94.5% for lambs, 63.5% for goats, and ...201424412959
molecular typing of campylobacter jejuni and campylobacter coli isolated from various retail meats by mlst and pfge.campylobacter species are one of the leading causes of foodborne disease in the united states. campylobacter jejuni and campylobacter coli are the two main species of concern to human health and cause approximately 95% of human infections. molecular typing methods, such as pulsed-field gel electrophoresis (pfge) and multilocus sequence typing (mlst) are often used to source track foodborne bacterial pathogens. the aim of the present study was to compare pfge and mlst in typing strains of c. jeju ...201428234305
report of ribosomal rna methylase gene erm(b) in multidrug-resistant campylobacter coli.campylobacter is a major foodborne enteric pathogen and macrolides are the drug of choice for the clinical therapy of campylobacteriosis. macrolide resistance among campylobacter compromises clinical treatment, is associated with adverse health events and is a significant public health concern. here, we report the first identification of a horizontally transferrable macrolide resistance mechanism in porcine campylobacter coli zc113 that is mediated by a ribosomal rna methylase, erm(b).201424335515
antimicrobial resistance of campylobacter jejuni and campylobacter coli from poultry in italy.this study was aimed at assessing the antimicrobial resistance (amr) of campylobacter isolates from broilers and turkeys reared in industrial farms in northern italy, given the public health concern represented by resistant campylobacters in food-producing animals and the paucity of data about this topic in our country. thirty-six campylobacter jejuni and 24 campylobacter coli isolated from broilers and 68 c. jejuni and 32 c. coli from turkeys were tested by disk diffusion for their susceptibili ...201424320689
specific pathogen-free pig herds also free from campylobacter?as specific pathogen-free (spf) pig herds are designed and managed to prevent specific pig diseases, it might be feasible to expand the list of micro-organisms also including zoonotic pathogens such as campylobacter coli as this agent has its origin in pigs. in a previous survey, 15 of 16 of spf herds were found free from human pathogenic yersinia enterocolitica. accordingly, three nucleus and seven multiplying herds were surveyed for campylobacter to investigate whether the norwegian spf pig py ...201524798507
[detection of biofilm formation by selected pathogens relevant to the food industry].detection of biofilm formation by microbial pathogens relevant to the food industry and comparison of biofilm formation under different conditions of culture.201526448305
prevalence and antimicrobial resistance of campylobacterjejuni and campylobacter coli from adult hospitalized patients with diarrhea in thailand.infection with campylobacter jejuni and c. coli are recognized as the major cause of bacterial gastroenteritis in humans.201526749592
eucast recommendations for antimicrobial susceptibility testing applied to the three main campylobacter species isolated in humans.antimicrobial susceptibility testing of campylobacter isolates is of great importance for treatment options especially in systemic diseases. the european committee for antimicrobial susceptibility testing (eucast) recently proposed epidemiological cut-offs (ecoffs) for a limited number of antimicrobial compounds and for campylobacter jejuni and campylobacter coli only. in the present study, the eucast method was used after minor modifications to define antimicrobial susceptibility patterns for, ...201526519770
efficacy of a typing scheme for campylobacter based on the combination of true and questionable crispr.this study evaluates an improved scheme for campylobacter genotyping based on the combination of true and questionable crispr (clustered regularly interspaced short palindromic repeats) elements. a total of 180 campylobacter strains (campylobacter jejuni n=93 and campylobacter coli n=87), isolated from neck skin and caecal content of broilers, poultry meat and sewage water were analysed. another 97 c. jejuni dna samples from cases of human campylobacteriosis were assessed. sixty-three genotypes ...201526518609
campylobacter in poultry: ecology and potential interventions.avian hosts constitute a natural reservoir for thermophilic campylobacter species, primarily campylobacter jejuni and campylobacter coli, and poultry flocks are frequently colonized in the intestinal tract with high numbers of the organisms. prevalence rates in poultry, especially in slaughter-age broiler flocks, could reach as high as 100% on some farms. despite the extensive colonization, campylobacter is essentially a commensal in birds, although limited evidence has implicated the organism a ...201526473668
campylobacter species in animal, food, and environmental sources, and relevant testing programs in canada.campylobacter species, particularly thermophilic campylobacters, have emerged as a leading cause of human foodborne gastroenteritis worldwide, with campylobacter jejuni, campylobacter coli, and campylobacter lari responsible for the majority of human infections. although most cases of campylobacteriosis are self-limiting, campylobacteriosis represents a significant public health burden. human illness caused by infection with campylobacters has been reported across canada since the early 1970s. m ...201526422448
risk of escherichia coli o157:h7, non-o157 shiga toxin-producing escherichia coli, and campylobacter spp. in food animals and their products in qatar.escherichia coli o157:h7, non-o157 e. coli, and campylobacter spp. are among the top-ranked pathogens that threaten the safety of food supply systems around the world. the associated risks and predisposing factors were investigated in a dynamic animal population using a repeat-cross-sectional study design. animal and environmental samples were collected from dairy and camel farms, chicken processing plants, and abattoirs and analyzed for the presence of these pathogens using a combination of bac ...201526408129
antimicrobial susceptibilities of campylobacter jejuni and campylobacter coli recovered from organic turkey farms in germany.the popularity of food produced from animals kept under an organic regimen has increased in recent years. in germany, turkey meat consumption has increased. despite several studies assessing the susceptibility of campylobacters to various antibiotics in poultry, no sufficient data exists regarding the antimicrobial resistance of campylobacters in organic-reared turkeys. this study provides information about antibiotic resistance in campylobacter isolated from turkeys reared on organic farms in g ...201526371330
campylobacter coli infection causing second trimester intrauterine growth restriction (iugr): a case report and review of the literature.campylobacter is a gram-negative, microaerophilic, curved rod and a normal resident of the gastrointestinal flora and may be the cause of disease in animals. transmission to humans occurs by ingestion of contaminated food or by direct contact with infected animals. in the past few decades, an increasing number of reports have implicated the presence of this organism in human abortions as well. an infectious mechanism due to primary placental inflammatory damage followed by secondary damage to th ...201526340229
antibiotic susceptibility of clinical isolates of campylobacter jejuni and campylobacter coli in ontario, canada during 2011-2013.a total of 219 clinical isolates of campylobacter spp. including 180 campylobacter jejuni and 39 campylobacter coli were assessed for in vitro antimicrobial susceptibility. resistance among c. coli was higher for ciprofloxacin (41% versus 30.80%), erythromycin (12.80% versus 3.90%) and lower for tetracycline (53.80% versus 64.60%) compared to c. jejuni.201526320936
development of a novel chromogenic medium for improved campylobacter detection from poultry samples.the presence of expanded-spectrum β-lactamase (esbl)-producing escherichia coli is a common problem in the isolation of campylobacter from poultry samples using conventional cefoperazone-based selective media. a novel chromogenic medium (cm-ht), based on modified charcoal cefoperazone deoxycholate agar (mccda), has been developed as a solution for improved campylobacter detection from poultry samples. although the basic components of cm-ht are the same as mccda, cm-ht uses both granular charcoal ...201526319731
are campylobacters now capable of carbo-loading?campylobacters are a leading cause of gastrointestinal morbidity worldwide and the majority of human infections are triggered by eating foods contaminated with campylobacter jejuni or campylobacter coli. campylobacters are equally notorious for their ability to mimic human glycoconjugate structures and for their capacity to synthesize both n- and o-linked glycoproteins. these species were once considered to be asaccharolytic, but it was recently shown that several strains possess a pathway for f ...201526259768
a transferable plasticity region in campylobacter coli allows isolates of an otherwise non-glycolytic food-borne pathogen to catabolize glucose.thermophilic campylobacter species colonize the intestine of agricultural and domestic animals commensally but cause severe gastroenteritis in humans. in contrast to other enteropathogenic bacteria, campylobacter has been considered to be non-glycolytic, a metabolic property originally used for their taxonomic classification. contrary to this dogma, we demonstrate that several campylobacter coli strains are able to utilize glucose as a growth substrate. isotopologue profiling experiments with (1 ...201526259566
high prevalence and genetic diversity of campylobacter jejuni in wild crows and pigeons.the occurrence, seasonal variation and genetic diversity of campylobacter spp. in pigeons and crows over a 1-year period were evaluated. campylobacter spp. were isolated from 166 (34.6 %) out of 480 wild bird faecal samples. the occurrence of campylobacter spp. in faecal samples was higher among crows (39.2 %) than pigeons (30.0 %), (p < 0.05). campylobacter jejuni was the most common species detected among wild bird faecal samples (98.2 %). meanwhile, campylobacter coli prevalence in wild bird ...201526228635
a new variant of cytolethal distending toxin in a clinical isolate of campylobacter hyointestinalis.increasing numbers of campylobacter hyointestinalis have been isolated from humans and animals with gastroenteritis, although the virulence mechanism of this species remains largely unknown. here, we show that c. hyointestinalis isolated from a patient with diarrhoea in thailand produced a novel variant of cytolethal distending toxin (cdt). sequencing of a 13 965 bp genomic region of c. hyointestinalis carrying the genes coding for ch-cdt revealed three orfs of 798, 804 and 537 bp, which code fo ...201526220191
adherence reduction of campylobacter jejuni and campylobacter coli strains to hep-2 cells by mannan oligosaccharides and a high-molecular-weight component of cranberry extract.campylobacter infections are a leading cause of human bacterial gastroenteritis in the united states and are a major cause of diarrheal disease throughout the world. colonization and subsequent infection and invasion of campylobacter require that the bacteria adhere to the surface of host cells. agents that inhibit adherence could be used prophylactically to reduce campylobacter carriage and infection. mannan oligosaccharides (mos) have been used as a feed supplement in livestock animals to impr ...201526219363
microarray on digital versatile disc for identification and genotyping of salmonella and campylobacter in meat products.highly portable, cost-effective, and rapid-response devices are required for the subtyping of the most frequent food-borne bacteria; thereby the sample rejection strategies and hygienization techniques along the food chain can be tailor-designed. here, a novel biosensor is presented for the generic detection of salmonella and campylobacter and the discrimination between their most prevalent serovars (salmonella enteritidis, salmonella typhimurium) and species (campylobacter jejuni, campylobacter ...201526198111
rapid detection of campylobacter jejuni, campylobacter coli, and campylobacter lari in fresh chicken meat and by-products in bangkok, thailand, using modified multiplex pcr.a multiplex pcr assay for simultaneous detection and differentiation of campylobacter jejuni, campylobacter coli, and campylobacter lari was developed and validated to assess the occurrence of these bacteria in fresh chicken meat and by-products in bangkok, thailand, by using a new combination of four previously published pcr primers for c. jejuni, c. coli, c. lari, and a universal 16s rdna gene as an internal control. the specificity was determined by using 13 strains of other bacteria. with pu ...201526197289
real-time pcr detection of campylobacter spp.: a comparison to classic culturing and enrichment.the major disadvantage of the current gold standard for detection of the food pathogen campylobacter, i.e. culturing, is the lengthy procedure. in this study we assessed the use of real-time pcr for detection of campylobacter. to this end, 926 poultry samples, taken from transport containers and broiler caeca in the netherlands in 2007, were subjected to three different real-time pcr detection methods: one targeting the campylobacter jejuni hipo gene, one targeting the campylobacter coli glya ge ...201526187833
restoring the selectivity of modified charcoal cefoperazone deoxycholate agar for the isolation of campylobacter species using tazobactam, a β-lactamase inhibitor.extended spectrum β-lactamase (esbl) producing escherichia coli have emerged as a contaminant on modified charcoal cefoperazone deoxycholate agar (mccda) when attempting to selectively isolate campylobacter spp. from poultry. e. coli are particularly problematic given their ability to grow under microaerophilic conditions and have been shown to outcompete campylobacter species making campylobacter detection or enumeration difficult. this paper recommends a novel method for restoring the selectiv ...201526119190
the evolution of campylobacter jejuni and campylobacter coli.the global significance of campylobacter jejuni and campylobacter coli as gastrointestinal human pathogens has motivated numerous studies to characterize their population biology and evolution. these bacteria are a common component of the intestinal microbiota of numerous bird and mammal species and cause disease in humans, typically via consumption of contaminated meat products, especially poultry meat. sequence-based molecular typing methods, such as multilocus sequence typing (mlst) and whole ...201526101080
comparative analysis of antimicrobial resistance and genetic diversity of campylobacter from broilers slaughtered in the current study, the relationship of campylobacter jejuni and campylobacter coli strains isolated at slaughter was investigated using comparative analysis of antimicrobial resistance (amr), virulence gene (vg) and pfge profiling. a total of 254 campylobacter isolates from poultry caeca and corresponding carcasses, including 139 c. jejuni and 115 c. coli strains were tested. the most prevalent resistance profiles observed in c. jejuni were ciprofloxacin, nalidixic acid and tetracycline (46 o ...201526092707
microbiological survey of birds of prey pellets.a microbiological survey of 73 pellets collected from different birds of prey species housed at the wildlife rescue and rehabilitation center of napoli (southern italy) was performed. pellets were analyzed by culture and biochemical methods as well as by serotyping and polymerase chain reaction. we isolated a wide range of bacteria some of them also pathogens for humans (i.e. salmonella enterica serotype typhimurium, campylobacter coli, escherichia coli o serogroups). this study highlights the p ...201526026881
prevalence of campylobacter spp. in retail chicken, turkey, pork, and beef meat in poland between 2009 and 2013.the purpose of the present study was to determine the prevalence of thermophilic campylobacter in poultry, pork, and beef meat at the retail level and to identify the main categories of meat representing the most significant reservoirs of campylobacter. a monitoring study was conducted throughout poland from 2009 to 2013. a total of 1,700 fresh meat samples were collected from supermarkets, large retail outlets, and smaller stores. thermophilic campylobacter species were detected in 690 (49.3%) ...201525951401
development of transient phage resistance in campylobacter coli against the group ii phage cp84.recently, there is a growing interest in the use of bacteriophages for pre- and post-harvest applications to reduce foodborne pathogens (including campylobacter) along the food chain. quantitative campylobacter reductions of up to three log10 units have been achieved by phage application. however, possible phage resistance might limit this approach. in campylobacter (c.) jejuni, phage resistance mechanisms have been described in detail but data on these mechanisms in c. coli are still missing. t ...201525876274
a five-year study on prevalence and antimicrobial resistance of campylobacter from poultry carcasses in poland.during 2009-2013 a total of 2114 swab samples collected from broiler carcasses in all 16 voivodeships (administrative districts) of poland were examined for the presence of campylobacter jejuni and campylobacter coli. the antimicrobial resistance of the isolates to ciprofloxacin, tetracycline and erythromycin using the mic method was also tested. it was found that 1151 (54.4%) carcasses were contaminated with campylobacter, with 50% of c. jejuni and c. coli species isolated from positive samples ...201525846926
effect of exposure to stress conditions on propidium monoazide (pma)-qpcr based campylobacter enumeration in broiler carcass rinses.campylobacter quantification by qpcr is unable to distinguish viable vs. dead cells in contrast to the culture-based iso 10272-2 reference method. propidium monoazide (pma) has been used to overcome this disadvantage. a campylobacter pma-qpcr enumeration method was evaluated for its consistency and compared to the culture-based enumeration for both artificially and natural contaminated broiler carcass rinses. the pma effect was further evaluated on stressed cells. five conditions, commonly encou ...201525791007
higher resistance of campylobacter coli compared to campylobacter jejuni at chicken order to compare the prevalence of campylobacter coli and campylobacter jejuni during the processing of broilers at slaughterhouse a total of 848 samples were analyzed during 2012 in southern spain. four hundred and seventy six samples were collected from cloaca, carcass surfaces and quartered carcasses. moreover, 372 environmental swabs from equipment and scalding water were collected. minimum inhibitory concentration (mic) to ciprofloxacin, erythromycin, streptomycin, tetracycline and genta ...201525770597
characteristics of metroxylon sagu resistant starch type iii as prebiotic substance.resistant starch type iii (rs3 ) was produced from sago (metroxylon sagu) and evaluated for its characteristics as a prebiotic. two rs3 samples designated sago rs and hcl-sago rs contained 35.71% and 68.30% rs, respectively, were subjected to hydrolyses by gastric juice and digestive enzymes and to absorption. both sago rs and hcl-sago rs were resistant to 180 min hydrolysis by gastric acidity at ph 1 to 4 with less than 0.85% hydrolyzed. both samples were also resistant toward hydrolysis by gas ...201525739421
identification of campylobacter species isolates with phenotypic methods and polymerase chain reaction.campylobacter jejuni (c. jejuni) and campylobacter coli (c. coli) are the most common bacterial causes of enterocolitis in humans. however, identification of the species level is not always possible using standard biochemical tests.201525731001
survival after cryogenic freezing of campylobacter species in ground turkey patties treated with polyphosphates.the use of polyphosphate-based marinades in the processing of poultry has been previously shown to increase the survival of campylobacter species present in the exudates derived from these products. this study investigates the effects that some of the same polyphosphates have on the survival of campylobacter species within a ground turkey product subjected to cryogenic freezing. ground turkey patties with two different polyphosphate formulations added in two different concentrations were artific ...201525710161
genotypes and antibiotic resistance of bovine campylobacter and their contribution to human campylobacteriosis.campylobacter jejuni and campylobacter coli are the most important bacterial causes of human gastroenteritis. chicken has been recognized as a major source for human infection, whereas cattle might also contribute to a lesser extent. however, there is a paucity of information available regarding campylobacter in swiss cattle and their role for human campylobacteriosis. to gain more information on genotypes and antibiotic resistance of bovine c. jejuni and c. coli and on their contribution to hum ...201525511436
antimicrobial activity of gallic acid against thermophilic campylobacter is strain specific and associated with a loss of calcium ions.gallic acid has been suggested as a potential antimicrobial for the control of campylobacter but its effectiveness is poorly studied. the minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) of gallic acid against campylobacter jejuni (n = 8) and campylobacter coli (n = 4) strains was determined. gallic acid inhibited the growth of five c. jejuni strains and three c. coli strains (mic: 15.63-250 μg ml(-1)). gallic acid was only bactericidal to two c. coli strains ( ...201525475290
comparison of anti-campylobacter activity of free thymol and thymol-β-d-glucopyranoside in absence or presence of β-glycoside-hydrolysing gut bacteria.thymol is a natural product that exhibits antimicrobial activity in vitro but in vivo results indicate that absorption within the proximal alimentary tract precludes its delivery to the distal gut. presently, the anti-campylobacter activity of thymol was compared against that of thymol-β-d-glucopyranoside, the latter being resistant to absorption. when treated with 1 mm thymol, campylobacter coli and jejuni were reduced during pure or co-culture with a β-glycoside-hydrolysing parabacteroides dis ...201525465999
prevalence, virulence, and antimicrobial resistance of campylobacter jejuni and campylobacter coli in white stork ciconia ciconia in poland.the aim of this study was to investigate the role of white stork ciconia ciconia as a potential reservoir of campylobacter spp. antimicrobial resistance and the presence of putative virulence genes of the isolates were also examined. a total of 398 white stork chicks sampled in western poland in habitats with high density of breeding were examined. rectal swabs were collected during breeding season 2009-2012 from storks developing in a relatively pure environment (odra meadows), in polluted area ...201525456607
characterization of campylobacter jejuni and campylobacter coli genotypes in poultry flocks by restriction fragment length polymorphism (rflp) analysis.we describe a simple, rapid, and discriminatory methodology that allows the routine molecular characterization of campylobacter jejuni and campylobacter coli isolates. the proposed approach is built on one of the earliest and simplest molecular typing methods ever, consisting on the analysis of the fragments of different lengths generated by digestion of homologous dna sequences with specific restriction endonucleases, a process known as restriction fragment length polymorphism (rflp) analysis. ...201525399105
[antimicrobial susceptibility and resistance mutations in campylobacter jejuni and c. coli isolates from human and meat sources].recently, there has been a marked increase in the number of reports of fluoroquinolone-resistant campylobacter jejuni and campylobacter coli. the aim of this study was to evaluate the prevalence of antimicrobial resistance and its genetic determinants in campylobacter species isolated from meat and human subjects in fukuoka prefecture, japan. between 2011 and 2013, 55 and 64 isolates were collected from meat (chicken meat and beef liver) and humans, respectively, in this prefecture. antimicrobia ...201526552121
novel gentamicin resistance genes in campylobacter isolated from humans and retail meats in the understand the molecular epidemiology of gentamicin-resistant campylobacter and investigate aminoglycoside resistance mechanisms.201525645207
rapid identification and classification of campylobacter spp. using laser optical scattering technology.campylobacter jejuni and campylobacter coli are the two important species responsible for most of the campylobacter infections in humans. reliable isolation and detection of campylobacter spp. from food samples are challenging due to the interferences from complex food substances and the fastidious growth requirements of this organism. in this study, a novel biosensor-based detection called bardot (bacterial rapid detection using optical scattering technology) was developed for high-throughput s ...201525583335
a receptor-binding protein of campylobacter jejuni bacteriophage nctc 12673 recognizes flagellin glycosylated with acetamidino-modified pseudaminic acid.bacteriophage receptor-binding proteins (rbps) confer host specificity. we previously identified a putative rbp (gp047) from the campylobacter lytic phage nctc 12673 and demonstrated that gp047 has a broader host range than its parent phage. while nctc 12673 recognizes the capsular polysaccharide (cps) of a limited number of campylobacter jejuni isolates, gp047 binds to a majority of c. jejuni and related campylobacter coli strains. in this study, we demonstrate that gp047 also binds to acapsula ...201525354466
free-living waterfowl as a source of zoonotic bacteria in a dense wild bird population area in northeastern spain.salmonella spp. and campylobacter spp. are zoonotic bacteria that represent an economic and public health concern worldwide. due to the difficulty to collect samples from free-living waterfowl, little is known on their importance as a reservoir of zoonotic agents. thus, a study was conducted to determine the prevalence, genotypic diversity and antimicrobial susceptibility of salmonella and campylobacter from waterfowl in ebro delta (northeastern spain), a geographical area with a dense wild bird ...201524112278
survival in water of campylobacter jejuni strains isolated from the slaughterhouse.campylobacter jejuni cause gastroenteritis in humans. the main transmission vector is the consumption or handling of contaminated chicken meat, since chicken can be colonized asymptomatically by c. jejuni. however, water has been implicated as the transmission vector in a few outbreaks. one possibility is the contamination of water effluent by c. jejuni originating from chicken farm. the ability of c. jejuni to be transmitted by water would be closely associated to its ability to survive in wate ...201526702388
smrt sequencing of the campylobacter coli bfr-ca-9557 genome sequence reveals unique methylation motifs.campylobacter species are the most prevalent bacterial pathogen causing acute enteritis worldwide. in contrast to campylobacter jejuni, about 5 % of campylobacter coli strains exhibit susceptibility to restriction endonuclease digestion by dpni cutting specifically 5'-g(m)atc-3' motifs. this indicates significant differences in dna methylation between both microbial species. the goal of the study was to analyze the methylome of a c. coli strain susceptible to dpni digestion, to identify its meth ...201526689587
molecular identification of campylobacter jejuni and coli from chicken, calves and dogs to determine its potential threat on human being.campylobacter is an emerging zoonotic pathogen and one of the leading cause of foodborne infection worldwide and it has been isolated from a variety of animal species. the aim of this study was to identify campylobacter jejuni and campylobacter coli from dogs, calves, and poultry using polymerase chain reaction (pcr).201527047055
a flagellar glycan-specific protein encoded by campylobacter phages inhibits host cell growth.we previously characterized a carbohydrate binding protein, gp047, derived from lytic campylobacter phage nctc 12673, as a promising diagnostic tool for the identification of campylobacter jejuni and campylobacter coli. we also demonstrated that this protein binds specifically to acetamidino-modified pseudaminic acid residues on host flagella, but the role of this protein in the phage lifecycle remains unknown. here, we report that gp047 is capable of inhibiting c. jejuni growth both on solid an ...201526694450
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