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comparative analysis of cytolethal distending toxin (cdt) genes among campylobacter jejuni, c. coli and c. fetus strains.the cytolethal distending toxin (cdt) gene clusters of campylobacter coli strain co1-243 and c. fetus strain col-187 were cloned and sequenced to understand the importance of cdt as a virulence factor. the cdt genes of c. coli and c. fetus consist of three closely linked genes termed cdta, cdtb, cdtc whose sizes are 774, 801, and 570 bp, and 702, 798, and 546 bp, respectively. the homologies of each subunit of cdt genes between c. jejuni and c. coli, c. jejuni and c. fetus, or c. coli and c. fet ...200717353111
genome sequence of a clinical isolate of campylobacter jejuni from thailand.campylobacter jejuni cg8486, which belongs to the hs4 complex, was isolated from a patient with inflammatory diarrhea in thailand. this strain caused a diarrheal disease in ferrets comparable to that caused by c. jejuni strain 81-176, but it was much less invasive for epithelial cells in vitro than 81-176. complete genome sequencing of cg8486 revealed a 1.65-mb genome that was very similar to the other two published genomes of clinical isolates of c. jejuni, the genomes of 81-176 and nctc 11168, ...200717438034
detection by pcr of eight groups of enteric pathogens in 4,627 faecal samples: re-examination of the english case-control infectious intestinal disease study (1993-1996).the english case-control infectious intestinal disease study (1993-1996) failed to detect an enteric pathogen or toxin in 49% of cases of gastroenteritis. in the present study, polymerase chain reaction (pcr) assays were applied to dna and cdna generated from 4,627 faecal samples from cases and controls archived during the original study for the detection of norovirus, rotavirus, sapovirus, campylobacter spp., salmonella spp., enteroaggregative escherichia coli, cryptosporidium spp., and giardia ...200717447091
prevalence, numbers, and subtypes of campylobacter jejuni and campylobacter coli in uncooked retail meat samples.a national quantitative survey of campylobacter jejuni and campylobacter coli in 1,011 uncooked retail meat samples (beef, unweaned veal, chicken, lamb and mutton, and pork) was undertaken from august 2003 to june 2004 to establish baseline proportionality data. the presence, number, and type of campylobacter present in each sample was assessed. prevalences of c. jejuni and c. coli were 89.1% in chicken, 9.1% in pork, 6.9% in lamb and mutton, 3.5% in beef, and 10% in unweaned veal. c. jejuni was ...200717388043
effect of macrolide usage on emergence of erythromycin-resistant campylobacter isolates in chickens.in this work we conducted both in vitro and in vivo experiments to examine the development and mechanisms of erythromycin (ery) resistance in campylobacter jejuni and campylobacter coli. in vitro plating revealed that both campylobacter species had similar but low spontaneous mutation frequencies (3 x 10(-9) to <5.41 x 10(-10)) for ery resistance. chickens infected with c. jejuni or c. coli were subjected to single or multiple treatments with medicated water containing tylosin (0.53 g/liter), wh ...200717353243
a phylogenomic analysis of the actinomycetales mce operons.the genome of mycobacterium tuberculosis harbors four copies of a cluster of genes termed mce operons. despite extensive research that has demonstrated the importance of these operons on infection outcome, their physiological function remains obscure. expanding databases of complete microbial genome sequences facilitate a comparative genomic approach that can provide valuable insight into the role of uncharacterized proteins.200717324287
helicobacter pylori is invasive and it may be a facultative intracellular organism.the pathogenicity of many bacteria colonizing the gastrointestinal tract often depends on their ability to gain access to cells that are normally non-phagocytic. helicobacter pylori colonizes the stomach of over half the world population and is the main cause of peptic ulcer disease and gastric cancer. it is generally considered to be a non-invasive pathogen present only in the lumen of the stomach and attached to gastric epithelial cells although a number of in vivo and in vitro studies have de ...200717388791
detection of campylobacter jejuni and campylobacter coli in chicken meat samples by real-time nucleic acid sequence-based amplification with molecular beacons.a nucleic acid sequence-based amplification (nasba) assay based on molecular beacons was used for real-time detection of campylobacter jejuni and campylobacter coli in samples of chicken meat. a set of specific primers and beacon probe were designed to target the 16s rrna of both species. the real-time nasba protocol including the rna isolation was valid for both of the cell suspensions in buffered saline and the artificially contaminated chicken meat samples. the presence of rrna could be corre ...200717395329
kinetics of the human antibody response against salmonella enterica serovars enteritidis and typhimurium determined by lipopolysaccharide enzyme-linked immunosorbent assay.two indirect enzyme-linked immunosorbent assays (elisas) were employed to measure levels of immunoglobulin g (igg), igm, and iga antibodies against salmonella in sera from 303 danish patients diagnosed by fecal culture with either salmonella enterica serovar enteritidis or salmonella enterica serovar typhimurium infections. the elisas were based on serovar enteritidis lipopolysaccharide (lps) and serovar typhimurium lps. the antibody levels were assessed approximately 1, 3, 6, and 12 months afte ...200717329442
relapsing campylobacter coli bacteremia with reactive arthritis in a patient with x-linked agammaglobulinemia.a patient genetically diagnosed with x-linked agammaglobulinemia repeatedly developed bacteremia due to campylobacter coli (c. coli) for one year and seven months in spite of immunoglobulin replacement therapy. throughout the clinical course, c. coli with identical genetic patterns was repeatedly isolated from both blood and stool cultures, thus indicating that the patient had latent intestinal infection. the bacteremia was always accompanied by reactive arthritis. since the immunoglobulin level ...200717473499
campylobacter in finnish organic laying hens in autumn 2003 and spring 2004.a total of 642 fecal samples and 360 table eggs from finnish organic laying hens were collected in autumn 2003 (19 farms) and spring 2004 (17 farms) and studied for the presence of campylobacter. in autumn, 84% of the farms were positive for campylobacter and in spring, 76%. the percentage of positive samples within a flock varied between 5 and 100%. in addition, campylobacter was isolated in a single eggshell sample. campylobacter jejuni was the species isolated most often, although campylobact ...200717495096
detection and genotyping of arcobacter and campylobacter isolates from retail chicken samples by use of dna oligonucleotide arrays.to explore the use of dna microarrays for pathogen detection in food, we produced dna oligonucleotide arrays to simultaneously determine the presence of arcobacter and the presence of campylobacter in retail chicken samples. probes were selected that target housekeeping and virulence-associated genes in both arcobacter butzleri and thermotolerant campylobacter jejuni and campylobacter coli. these microarrays showed a high level of probe specificity; the signal intensities detected for a. butzler ...200717416693
a helicobacter hepaticus catalase mutant is hypersensitive to oxidative stress and suffers increased dna damage.catalase (kata) is known to play an important role in oxidative stress resistance in many bacterial species and a homologue exists in helicobacter hepaticus, a member of the enterohepatic helicobacter species. here, a kata mutant was constructed by insertional mutagenesis and its oxidative stress phenotype was investigated. catalase activity was readily detected [196 units (mg protein crude cell extract)(-1)] in the wild-type, whereas the mutant strain was deficient in, but not devoid of, activi ...200717374900
susceptibility of bacteria isolated from pigs to tiamulin and enrofloxacin metabolites.susceptibilities to metabolites of tiamulin (tia) and enrofloxacin (enr) were tested using selected bacteria with previously defined minimal inhibitory concentrations (mic). the tia metabolites tested were: n-deethyl-tiamulin (dtia), 2beta-hydroxy-tiamulin (2beta-htia) and 8alpha-hydroxy-tiamulin (8alpha-htia), and the enr metabolites were: ciprofloxacin (cip) and enrofloxacin n-oxide (enr-n). bacteria, all of porcine origin, were selected as representatives of bacterial infections (staphylococc ...200717194550
emerging fluoroquinolone and macrolide resistance of campylobacter jejuni and campylobacter coli isolates and their serotypes in thai children from 1991 to 2000.this study investigated fluoroquinolone, macrolide resistances and serotype distributions among campylobacter jejuni and campylobacter coli isolated from children in bangkok and rural settings during 1991-2000. phenotypic identification, serotyping, and susceptibility testing were performed by standard microbiological procedures. the predominant serotypes of c. jejuni were lior 36, 2 and 4 and of c. coli were lior 8, 29 and 55. resistance to nalidixic acid increased significantly during 1991-200 ...200717306054
conjugative transfer of chromosomally encoded antibiotic resistance from helicobacter pylori to campylobacter jejuni.many strains of helicobacter pylori are naturally competent for transformation and able to transfer chromosomal dna among different isolates using a conjugation-like mechanism. in this study, we sought to determine whether h. pylori can transfer dna into campylobacter jejuni, a closely related species of the campylobacterales group. to monitor the transfer, a chromosomally encoded streptomycin resistance cassette prearranged by a specific mutation in the rpsl gene of h. pylori was used. mating o ...200717135441
identification of genomic differences between campylobacter jejuni subsp. jejuni and c. jejuni subsp. doylei at the nap locus leads to the development of a c. jejuni subspeciation multiplex pcr method.the human bacterial pathogen campylobacter jejuni contains two subspecies: c. jejuni subsp. jejuni (cjj) and c. jejuni subsp. doylei (cjd). although cjd strains are isolated infrequently in many parts of the world, they are obtained primarily from human clinical samples and result in an unusual clinical symptomatology in that, in addition to gastroenteritis, they are associated often with bacteremia. in this study, we describe a novel multiplex pcr method, based on the nitrate reductase (nap) lo ...200717328805
conjugative transfer of chromosomally encoded antibiotic resistance from helicobacter pylori to campylobacter jejuni.many strains of helicobacter pylori are naturally competent for transformation and able to transfer chromosomal dna among different isolates using a conjugation-like mechanism. in this study, we sought to determine whether h. pylori can transfer dna into campylobacter jejuni, a closely related species of the campylobacterales group. to monitor the transfer, a chromosomally encoded streptomycin resistance cassette prearranged by a specific mutation in the rpsl gene of h. pylori was used. mating o ...200717135441
campylobacter reactive arthritis: a systematic review.to review the literature on the epidemiology of campylobacter-associated reactive arthritis (rea).200717360026
novel campylobacter isolation method using hydrophobic grid membrane filter and semisolid medium.culture procedures for isolation of thermophilic campylobacters from food matrices are complex, labor intensive, and time-consuming. most available methods include the use of antibiotics as selective agents to prevent the growth of competing microflora. a simple procedure for isolation of thermophilic campylobacters after enrichment in rosef's enrichment broth was developed using a hydrophobic grid membrane filter (hgmf) on semisolid medium (ssm). ssm contains no antibiotics, and the hgmf physic ...200717340869
prevalence of campylobacter spp. in cattle in finland and antimicrobial susceptibilities of bovine campylobacter jejuni strains.the study investigated the prevalence of campylobacter spp. in finnish cattle at slaughter and carcass contamination after slaughter. during the period january to december 2003, bovine rectal fecal samples (n=952) and carcass surface samples (n=948) from 12 out of 15 finnish slaughterhouses were examined. in total, campylobacters were detected in 31.1% of fecal samples and in 3.5% of carcass surface samples. campylobacter jejuni was isolated from 19.5%, campylobacter coli from 2.2%, and presumpt ...200717369335
characterization of genetically matched isolates of campylobacter jejuni reveals that mutations in genes involved in flagellar biosynthesis alter the organism's virulence potential.phenotypic and genotypic evidence suggests that not all campylobacter jejuni isolates are pathogenic for humans. we hypothesized that differences in gene content or gene expression alter the degree of pathogenicity of c. jejuni isolates. a c. jejuni isolate (turkey) recovered from a turkey and a second c. jejuni isolate (cs) recovered from a chicken differed in their degrees of in vitro and in vivo virulence. the c. jejuni turkey isolate invaded int 407 human epithelial cells and secreted the ci ...200717369342
targeted metabolomics analysis of campylobacter coli vc167 reveals legionaminic acid derivatives as novel flagellar glycans.glycosylation of campylobacter flagellin is required for the biogenesis of a functional flagella filament. recently, we used a targeted metabolomics approach using mass spectrometry and nmr to identify changes in the metabolic profile of wild type and mutants in the flagellar glycosylation locus, characterize novel metabolites, and assign function to genes to define the pseudaminic acid biosynthetic pathway in campylobacter jejuni 81-176 (mcnally, d. j., hui, j. p., aubry, a. j., mui, k. k., gue ...200717371878
evaluation of an enzyme immunoassay-based stool antigen test to detect campylobacter jejuni and campylobacter coli.an enzyme immunoassay-based antigen test (ridascreen campylobacter; r-biopharm, darmstadt, germany) was evaluated for the detection of campylobacter jejuni and campylobacter coli in 1050 clinical stool samples as compared with culture on selective medium. after routine inoculation for salmonella, shigella, yersinia, aeromonas, plesiomonas, and campylobacter, the same swab specimens were used for the antigen test. the positivity rate for campylobacter was 9.3% in culture, and the antigen test gav ...200717300899
evaluating antibiotics for use in medicine using a poloxamer biofilm model.wound infections, due to biofilms, are a constant problem because of their recalcitrant nature towards antibiotics. appropriate antibiotic selection for the treatment of these biofilm infections is important. the traditional in vitro disc diffusion method for antibiotic selection uses bacterial cultures grown on agar plates. however, the form of bacterial growth on agar is not representative of how bacteria grow in wounds and other tissue sites as here bacteria grow naturally in a biofilm. the a ...200717302978
antimicrobial resistance of arcobacter and campylobacter from broiler carcasses.the antimicrobial resistance of arcobacter (n=174) and campylobacter (n=215) isolated from broiler carcasses in a us poultry processing plant was examined. for arcobacter, 93.7% (n=163) were resistant to one or more antimicrobials and 71.8% (n=125) were resistant to two or more antimicrobials. for campylobacter, 99.5% (n=214) were resistant to one or more antimicrobials and 28.4% (n=61) were resistant to two or more antimicrobials. arcobacter butzleri isolates were particularly resistant to clin ...200717303391
a genotyping investigation of the colonization of piglets by campylobacter coli in the first 10 weeks of life.to examine the campylobacter genotypes colonizing a litter of piglets during the first 10 weeks of life and compare them with those of the sow.200717381734
characterization of campylobacter jejuni biofilms under defined growth conditions.campylobacter jejuni is a major cause of human diarrheal disease in many industrialized countries and is a source of public health and economic burden. c. jejuni, present as normal flora in the intestinal tract of commercial broiler chickens and other livestock, is probably the main source of human infections. the presence of c. jejuni in biofilms found in animal production watering systems may play a role in the colonization of these animals. we have determined that c. jejuni can form biofilms ...200717259368
the orphan response regulator hp1021 of helicobacter pylori regulates transcription of a gene cluster presumably involved in acetone metabolism.helicobacter pylori is a gastric pathogen for which no nonhuman reservoir is known. in accordance with the tight adaptation to its unique habitat, the human stomach, h. pylori is endowed with a very restricted repertoire of regulatory proteins. nevertheless, the three complete two-component systems of h. pylori were shown to be involved in the regulation of important virulence traits like motility and acid resistance and in the control of metal homeostasis. hp1021 is an orphan response regulator ...200717220217
evaluation of genetic markers and molecular typing methods for prediction of sources of campylobacter jejuni and c. coli infections.campylobacter jejuni and c. coli isolates from poultry, cattle, and humans were studied using pulsed-field gel electrophoresis (pfge) and pcr of candidate livestock-associated marker genes. human isolates showed 5.7 and 61% overlap with cattle and poultry isolates, respectively, by use of pfge. no unambiguous association was found between marker genes (the cj1321 and cj1324 genes) and livestock-associated isolates.200717220252
dissemination of antimicrobial resistant strains of campylobacter coli and campylobacter jejuni among cattle in washington state and california.the purpose of this study was to investigate the genetic similarity of campylobacter jejuni and campylobacter coli with similar antimicrobial resistance phenotypes, isolated from cattle on different farms and at different times, in order to evaluate the possible existence of disseminated antimicrobial resistant clones. pfge after smai and kpni restriction identified 23 and 16 distinct pfge patterns among 29 c. jejuni and 66 c. coli isolates, respectively. in c. coli, 51 (77%) of the resistant is ...200717321701
peptide transport in helicobacter pylori: roles of dpp and opp systems and evidence for additional peptide transporters.despite research into the nutritional requirements of helicobacter pylori, little is known regarding its use of complex substrates, such as peptides. analysis of genome sequences revealed putative abc-type transporter genes for dipeptide (dppabcdf) and oligopeptide (oppabcd) transport. genes from each system were pcr amplified, cloned, and disrupted by cassette insertion either individually (dppa, dppb, dppc, oppa, oppb, and oppc) or to create double mutants (dppa oppa, dppb oppb, dppb dppc, and ...200717322309
identification of putative zinc hydrolase genes of the metallo-beta-lactamase superfamily from campylobacter jejuni.dna fragments encoding two putative zinc-dependent hydrolases, designated glx2-1 and glx2-2, from a clinical isolate of campylobacter jejuni, strain 012, were cloned and sequenced. glx2-1 was encoded by a sequence of 798 bp and glx2-2 by a sequence of 597 bp. the amino acid sequences deduced from c. jejuni dna showed 99% and 100% identity, respectively, to putative zinc hydrolases reported from c. jejuni atcc strain 11168, and also shared identity (28-43%) with several hypothetical conserved pro ...200717266723
succession in the intestinal microbiota of preadolescent turkeys.in the present study, automated ribosomal intergenic spacer analysis (arisa), library sequence analysis, real-time pcr detection of bacteroides uniformis and campylobacter coli and dot-blot hybridizations of clostridiaceae were used to identify trends in microbial colonization of the ceca of male turkeys. two separate trials were performed with six and five birds, respectively. arisa community profiles identified a period of community transition at week 12 of age in both trials. a significant in ...200717284250
new consensus guidelines from the clinical and laboratory standards institute for antimicrobial susceptibility testing of infrequently isolated or fastidious bacteria.the clinical and laboratory standards institute (clsi) recently published a new laboratory guideline for antimicrobial susceptibility testing of infrequently encountered or fastidious bacteria not covered in previous clsi publications. the organisms include aeromonas species, bacillus species, and vibrio species that may cause infections following environmental exposure. fastidious organisms that may cause endocarditis or medical device infections include abiotrophia and granulicatella species; ...200717173232
the absence of intervening sequences in 23s rrna genes of campylobacter coli isolates from turkeys is a unique attribute of a cluster of related strains which also lack resistance to erythromycin.certain campylobacter strains harbor a transcribed intervening sequence (ivs) in their 23s rrna genes. following transcription, the ivs is excised, leading to fragmentation of the 23s rrna. the origin and possible functions of the ivs are unknown. furthermore, the distribution of ivs-harboring strains within campylobacter populations is poorly understood. in this study, 104 strains of campylobacter coli from turkeys, representing 27 different multilocus sequence typing-based sequence types (sts) ...200717189444
comparison of disk diffusion and agar dilution methods for erythromycin and ciprofloxacin susceptibility testing of campylobacter jejuni subsp. jejuni.disk diffusion was a reliable, easy, and inexpensive method for testing the susceptibility of campylobacter jejuni to erythromycin (215 susceptible and 45 resistant isolates) and to ciprofloxacin (154 susceptible, two intermediate, and 124 resistant isolates) using, respectively, an erythromycin disk and ciprofloxacin and nalidixic acid disks.200717261628
campylobacter coli isolates derived from chickens in senegal: diversity, genetic exchange with campylobacter jejuni and quinolone resistance.we used the multilocus sequence typing (mlst) method to study the genetic diversity of campylobacter coli isolated from chickens in senegal, and to check the presence of genetic exchange with campylobacter jejuni. in addition, we assessed the resistance of the isolates to ciprofloxacin and nalidixic acid, and their gyra sequences. mlst revealed a low level of diversity and the absence of lineages among c. coli isolates. in addition, an exchange of alleles with c. jejuni was found. twenty percent ...200717258435
antisense rna modulation of alkyl hydroperoxide reductase levels in helicobacter pylori correlates with organic peroxide toxicity but not infectivity.much of the gene content of the human gastric pathogen helicobacter pylori ( approximately 1.7-mb genome) is considered essential. this view is based on the completeness of metabolic pathways, infrequency of nutritional auxotrophies, and paucity of pathway redundancies typically found in bacteria with larger genomes. thus, genetic analysis of gene function is often hampered by lethality. in the absence of controllable promoters, often used to titrate gene function, we investigated the feasibilit ...200717337572
comparison of the localization and post-translational modification of campylobacter coli cjac and its homolog from campylobacter jejuni, cj0734c/hisj.campylobacter is an asaccharolytic microorganism which uses amino acids as a source of carbon and energy. cjac/hisj is a ligand-binding protein, a component of the abc transport system. campylobacter cjac/hisj is post-translationally modified by glycosylation. the number of glycosylation motifs present in the cjac protein is species-specific. c. coli cjac has two and c. jejuni one motif (e/dxnys/t) which serves as a glycan acceptor. although the two c. coli cjac motifs have identical amino-acid ...200717351673
identification of helicobacter pylori genes that contribute to stomach colonization.chronic infection of the human stomach by helicobacter pylori leads to a variety of pathological sequelae, including peptic ulcer and gastric cancer, resulting in significant human morbidity and mortality. several genes have been implicated in disease related to h. pylori infection, including the vacuolating cytotoxin and the cag pathogenicity island. other factors important for the establishment and maintenance of infection include urease enzyme production, motility, iron uptake, and stress res ...200717101654
antibiotic resistance of campylobacter jejuni and campylobacter coli clinical isolates from poland.we tested 102 campylobacter jejuni and 6 campylobacter coli clinical isolates from poland. all were susceptible to erythromycin. among the tested c. jejuni isolates 55.9% and 13.7% were resistant to ciprofloxacin and tetracycline, respectively. replacement of thr86 with ile in gyra and a plasmid-borne tet(o) gene were the main resistance mechanisms for fluoroquinolones and tetracycline, respectively.200717210776
identification of helicobacter pylori genes that contribute to stomach colonization.chronic infection of the human stomach by helicobacter pylori leads to a variety of pathological sequelae, including peptic ulcer and gastric cancer, resulting in significant human morbidity and mortality. several genes have been implicated in disease related to h. pylori infection, including the vacuolating cytotoxin and the cag pathogenicity island. other factors important for the establishment and maintenance of infection include urease enzyme production, motility, iron uptake, and stress res ...200717101654
cryptic plasmids isolated from campylobacter strains represent multiple, novel incompatibility groups.three small, cryptic plasmids from the multi-drug-resistant (mdr) campylobacter coli strain rm2228 and one small, cryptic plasmid from the mdr campylobacter jejuni strain rm1170 were sequenced and characterized. pcc2228-1 has some similarity to firmicutes repl family plasmids that replicate via a rolling-circle mechanism. pcc2228-2 is a theta-replicating, iteron-containing plasmid (icp) that is a member of the same incompatibility (inc) group as previously described campylobacter shuttle vectors ...200717064774
specific detection of campylobacter jejuni from faeces using single nucleotide polymorphisms.specimens of human faeces were tested by a rapid strategy for detection of campylobacter jejuni lineages by the presence of specific single nucleotide polymorphisms (snps) based on the c. jejuni multi locus sequence typing (mlst) scheme. this strategy was derived from analysis of the mlst databases to identify clonal complex specific snps followed by the design of real-time pcr assays to enable identification of six major c. jejuni clonal complexes associated with cases of human infection. the o ...200717109769
specific detection of campylobacter jejuni from faeces using single nucleotide polymorphisms.specimens of human faeces were tested by a rapid strategy for detection of campylobacter jejuni lineages by the presence of specific single nucleotide polymorphisms (snps) based on the c. jejuni multi locus sequence typing (mlst) scheme. this strategy was derived from analysis of the mlst databases to identify clonal complex specific snps followed by the design of real-time pcr assays to enable identification of six major c. jejuni clonal complexes associated with cases of human infection. the o ...200717109769
distribution and ecology of campylobacters in coastal plain streams (georgia, united states of america).campylobacter is the leading cause of bacterium-associated diarrhea in the united states and most developed countries. while this disease is considered a food-borne disease, many clinical cases cannot be linked to a food source. in rural and agrarian areas environmental transmission may be an important factor contributing to case loads. here we investigated the waterborne prevalence of campylobacters in a mixed-use rural watershed in the coastal plain of southern georgia (united states). six sit ...200717172457
distribution and ecology of campylobacters in coastal plain streams (georgia, united states of america).campylobacter is the leading cause of bacterium-associated diarrhea in the united states and most developed countries. while this disease is considered a food-borne disease, many clinical cases cannot be linked to a food source. in rural and agrarian areas environmental transmission may be an important factor contributing to case loads. here we investigated the waterborne prevalence of campylobacters in a mixed-use rural watershed in the coastal plain of southern georgia (united states). six sit ...200717172457
clonal complexes of campylobacter jejuni identified by multilocus sequence typing are reliably predicted by restriction fragment length polymorphism analyses of the flaa gene.multilocus sequence typing (mlst) has provided important new insights into the population structure of campylobacter jejuni and is rapidly becoming the gold standard for typing this species. however, the methodology is comparatively costly and slow to perform for the routine surveillance testing of large numbers of isolates required by public health laboratories. restriction fragment length polymorphism analysis of the flaa gene (rflp-flaa) and sequencing of the variable region in the fla locus ...200717093018
longitudinal study of finnish campylobacter jejuni and c. coli isolates from humans, using multilocus sequence typing, including comparison with epidemiological data and isolates from poultry and cattle.we describe a study on the application of multilocus sequence typing for the analysis of campylobacter jejuni and c. coli isolates from human domestically acquired infections in the helsinki-uusimaa area of finland in 1996, 2002, and 2003. in addition, isolates from poultry meat and fecal samples of cattle from the seasonal peak (july to september) in 2003 were included in the study. in total, 361 finnish c. jejuni and c. coli strains were typed. sequence type 45 (st-45) (45%), st-21 (21%), and ...200717085689
comparison of antimicrobial susceptibility testing of campylobacter spp. by the agar dilution and the agar disk diffusion methods.the correlation and the level of agreement between the standardized agar dilution and the agar disk diffusion methods for antimicrobial susceptibility testing of campylobacter were investigated. a high-level agreement between the two methods was evident for aminoglycosides and fluoroquinolones, while a low-level agreement was observed for other antibiotics.200717122005
comparison of antimicrobial susceptibility testing of campylobacter spp. by the agar dilution and the agar disk diffusion methods.the correlation and the level of agreement between the standardized agar dilution and the agar disk diffusion methods for antimicrobial susceptibility testing of campylobacter were investigated. a high-level agreement between the two methods was evident for aminoglycosides and fluoroquinolones, while a low-level agreement was observed for other antibiotics.200717122005
clonal complexes of campylobacter jejuni identified by multilocus sequence typing are reliably predicted by restriction fragment length polymorphism analyses of the flaa gene.multilocus sequence typing (mlst) has provided important new insights into the population structure of campylobacter jejuni and is rapidly becoming the gold standard for typing this species. however, the methodology is comparatively costly and slow to perform for the routine surveillance testing of large numbers of isolates required by public health laboratories. restriction fragment length polymorphism analysis of the flaa gene (rflp-flaa) and sequencing of the variable region in the fla locus ...200717093018
detection of thermotolerant campylobacter in ostriches (struthio camelus) in italy.during the period january 2004 to december 2005 four different ostrich farms were investigated to evaluate the presence of thermotolerant campylobacter. a total of 150 ostriches were examined and all were found to be clinically healthy. campylobacter spp. were isolated from 60/150 cloacal swabs. among the isolates, 48 were identified as campylobacter jejuni whereas the remainder were identified as campylobacter coli. campylobacter lari was not isolated. the ostriches, although apparently in a he ...200716959506
sensitive and rapid detection of cholera toxin-producing vibrio cholerae using a loop-mediated isothermal amplification.vibrio cholerae is widely acknowledged as one of the most important waterborne pathogen causing gastrointestinal disorders. cholera toxin (ct) is a major virulence determinant of v. cholerae. detection of ct-producing v. cholerae using conventional culture-, biochemical- and immunological-based assays is time-consuming and laborious, requiring more than three days. thus, we developed a novel and highly specific loop-mediated isothermal amplification (lamp) assay for the sensitive and rapid detec ...200818547441
characterization of two campylobacter jejuni strains for use in volunteer experimental-infection studies.the development of vaccines against campylobacter jejuni would be facilitated by the ability to perform phase ii challenge studies. however, molecular mimicry of the lipooligosaccharide (los) of most c. jejuni strains with human gangliosides presents safety concerns about the development of guillain-barré syndrome. clinical isolates of c. jejuni that appeared to lack genes for the synthesis of ganglioside mimics were identified by dna probe analyses. two clinical isolates from southeast asia (st ...200818809665
antimicrobial resistance of campylobacter coli isolates from swine.eighty-three swine isolates of campylobacter coli were tested for mechanisms underlying resistance to nalidixic acid, ciprofloxacin, erythromycin and tetracycline. four isolates harbored class 1 integrons but none carried class 2 and 3 integrons. most of the tetracycline-resistant isolates (97%) possessed tet(o). a thr-86-ile substitution in gyra and an a-2230-g mutation in 23s rrna were the main resistance mechanisms for quinolones and erythromycin, respectively.200818945505
role of the helicobacter hepaticus flagellar sigma factor flia in gene regulation and murine colonization.the enterohepatic helicobacter species helicobacter hepaticus colonizes the murine intestinal and hepatobiliary tract and is associated with chronic intestinal inflammation, gall stone formation, hepatitis, and hepatocellular carcinoma. thus far, the role of h. hepaticus motility and flagella in intestinal colonization is unknown. in other, closely related bacteria, late flagellar genes are mainly regulated by the sigma factor flia (sigma(28)). we investigated the function of the h. hepaticus fl ...200818689480
[evaluation of antigenic properties of campylobacter jejuni and campylobacter coli proteins in a western-immunoblot].campylobacter jejuni and campylobacter coli are the most common bacterial cause for acute diarrheal illnesses in developed countries. the aim of this study was to evaluate the antigenic properties of campylobacterjejuni and campylobacter coli proteins in western-blot assay. whole-cell components of campulobacter jejuni and campylobacter coli were separated by sodium dodecyl sulfate-polyacrylamide gel electroforesis. using this method we detected in all seven c. jejuni strains 21 peptides migrati ...200818819448
pediatric campylobacteriosis in northern taiwan from 2003 to 2005.there has been a marked increase in the incidence of, and concern regarding, human campylobacter jejuni and c. coli infections worldwide during the last decade. as the highest infectious disease control apparatus in taiwan, we aimed to describe the character of campylobacter isolates from infected children, as well as basic information about the patients, from december 2003 to february 2005.200818973701
assessment of the stability of cell-surface components of microorganisms by maldi-tof-ms following preservation on lenticule discs.strains representing the species campylobacter coli, escherichia coli, listeria monocytogenes, pseudomonas aeruginosa, salmonella enterica, and staphylococcus aureus were randomly selected to assess the consistency of cells preserved on lenticule discs to those archived in traditional freeze-dried ampoules. each matched pair was cultured using identical conditions and analysed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (ms) to profile the surface-associated m ...200818717884
molecular mechanisms involved in vascular interactions of the lyme disease pathogen in a living host.hematogenous dissemination is important for infection by many bacterial pathogens, but is poorly understood because of the inability to directly observe this process in living hosts at the single cell level. all disseminating pathogens must tether to the host endothelium despite significant shear forces caused by blood flow. however, the molecules that mediate tethering interactions have not been identified for any bacterial pathogen except e. coli, which tethers to host cells via a specialized ...200818833295
demographic determinants for campylobacter infection in england and wales: implications for future epidemiological studies.despite a significant public health burden the epidemiology of human campylobacter infection remains blurred. the identification of demographic determinants for campylobacter infection is therefore essential for identifying potential areas for intervention. demographic data from an active, population-based sentinel surveillance system for campylobacter infection (from 2000 until 2003, n=15 907) were compared with appropriate denominator data from the 2001 united kingdom census. incidence was hig ...200819000328
the origin of a novel gene through overprinting in escherichia coli.overlapped genes originate by a) loss of a stop codon among contiguous genes coded in different frames; b) shift to an upstream initiation codon of one of the contiguous genes; or c) by overprinting, whereby a novel open reading frame originates through point mutation inside an existing gene. although overlapped genes are common in viruses, it is not clear whether overprinting has led to new genes in prokaryotes.200818226237
development and evaluation of a loop-mediated isothermal amplification assay for rapid and simple detection of campylobacter jejuni and campylobacter coli.we developed a loop-mediated isothermal amplification (lamp) assay for the rapid and simple detection of campylobacter jejuni and campylobacter coli. the assay provides a specific lamp product for each of these two species. the assay correctly identified 65 c. jejuni and 45 c. coli strains, but not 75 non-c. jejuni/coli strains. the sensitivity of the lamp assay for c. jejuni and c. coli in spiked human stool specimens was 5.6 x 10(3) c.f.u. g(-1) (1.4 c.f.u. per test tube) and 4.8 x 10(3) c.f.u ...200818349363
functional characterization and mutagenesis of the proposed behavioral sensor tlpd of helicobacter pylori.helicobacter pylori requires flagellar motility and chemotaxis to establish and maintain chronic infection of the human stomach. the ph gradient in the stomach mucus is essential for bacterial orientation and guides the bacterium toward a narrow layer of the mucus, suggesting that h. pylori is capable of energy sensing or taxis. in the present study, h. pylori wild-type behavior in a temporal swimming assay could be altered by electron transport inhibitors, indicating that a connection between m ...200818245281
comparison of antimicrobial resistance of campylobacter jejuni and campylobacter coli isolated from humans and chicken carcasses in poland.campylobacter-associated gastroenteritis remains an important cause of morbidity worldwide, and some evidence suggests that poultry is an important source of this foodborne infection in humans. this study was conducted to analyze the prevalence and genetic background of resistance of 149 campylobacter jejuni and 54 campylobacter coli strains isolated from broiler chicken carcasses and from stool samples of infected children in poland from 2003 through 2005. nearly all isolates were susceptible t ...200818389707
extended sequence typing of campylobacter spp., united kingdom.supplementing campylobacter spp. multilocus sequence typing with nucleotide sequence typing of 3 antigen genes increased the discriminatory index achieved from 0.975 to 0.992 among 620 clinical isolates from oxfordshire, united kingdom. this enhanced typing scheme enabled identification of clusters and retained data required for long-range epidemiologic comparisons of isolates.200818826829
restoration of flagellar biosynthesis by varied mutational events in campylobacter jejuni.both a complex regulatory cascade involving the flgsr two-component system and phase variation control expression of sigma(54)-dependent flagellar genes in campylobacter jejuni. in this study, mutational mechanisms influencing production of the flgs histidine kinase were discovered. random non-motile, non-flagellated flgs variants were impaired for growth in the chick intestinal tract. spontaneous revertants restored for flagellar biosynthesis, gene expression, and motility identified by in vivo ...200818761684
prevalence of virulence genes and cytolethal distending toxin production in campylobacter jejuni isolates from diarrheal patients in bangladesh.from 300 stool samples, 58 campylobacter strains were isolated by standard microbiological and biochemical methods. of these, 40 strains were identified as campylobacter jejuni and 5 as campylobacter coli. the presence of flaa (100%), cadf (100%), racr (100%), dnaj (100%), plda (100%), ciab (95%), virb11 (0%), ceue (82.5%), cdta (97.5%), cdtb (97.5%), cdtc (97.5%), and wlan (7.5%) genes was detected in c. jejuni by pcr. all c. jejuni strains but one produced cytolethal distending toxin in a hela ...200818287317
helicobacter hepaticus hhgi1 is a pathogenicity island associated with typhlocolitis in b6.129-il10 tm1cgn mice.helicobacter hepaticus strain 3b1 (h. hepaticus) contains a genomic island of approximately 71 kb, hhgi1, with some of the common features shared among known bacterial pathogenicity islands. in this study, we characterized the pathogenic potential of hhgi1 by infecting b6.129-il10 tm1cgn (il10-/-) mice with an isogenic mutant (namely hhpaid1) lacking 19 predicted genes within hhgi1. in contrast to h. hepaticus (p<0.001), hhpaid1 did not cause typhlocolitis and hyperplasia in il10-/- mice. coloni ...200818538610
expression, purification, and structural characterization of cfra, a putative iron transporter from campylobacter jejuni.the gene for the campylobacter ferric receptor (cfra), a putative iron-siderophore transporter in the enteric food-borne pathogen campylobacter jejuni, was cloned, and the membrane protein was expressed in escherichia coli, affinity purified, and then reconstituted into model lipid membranes. fourier transform infrared spectra recorded from the membrane-reconstituted cfra are similar to spectra that have been recorded from other iron-siderophore transporters and are highly characteristic of a be ...200818556796
genomic evidence for interspecies acquisition of chromosomal dna from campylobacter jejuni by campylobacter coli strains of a turkey-associated clonal group (cluster ii).previous multilocus sequence typing studies of campylobacter coli from meat animals identified an unusual cluster of strains, primarily from turkeys, termed "cluster ii" and characterized by the presence of the c. jejuni aspa103 allele. to characterize the extent of genomic input from c. jejuni in the aspa region of cluster ii c. coli, we sequenced the 6.1 kb genomic region upstream of and including aspa from two turkey-derived cluster ii strains (c. coli 6979 and c. coli 7474, of st-1150 and st ...200818673073
competing isogenic campylobacter strains exhibit variable population structures in vivo.consumption of poultry contaminated with campylobacter jejuni is a risk factor for human gastrointestinal disease. the rational development of control strategies for campylobacter within chickens requires an understanding of the colonization process at the molecular and population levels, both within and between hosts. experiments employing competing strains of campylobacter have been used to investigate colonization. implicit in these studies is the assumption that the behavior of competing str ...200818424530
longitudinal study of the molecular epidemiology of campylobacter jejuni in cattle on dairy farms.multilocus sequence typing (mlst), an accurate and phylogenetically robust characterization method for population studies of campylobacter, was applied to campylobacter jejuni isolates (n = 297) from the fecal samples of cattle from five dairy farms in cheshire, united kingdom, collected throughout 2003. the population dynamics of the c. jejuni strains, as identified by the occurrence of sequence types and clonal complexes, demonstrated variations within and between cattle populations over time. ...200818424539
the campylobacter jejuni/coli cjaa (cj0982c) gene encodes an n-glycosylated lipoprotein localized in the inner membrane.the campylobacter coli 72dz/92 cjaa gene (orthologue of cj0982c of c. jejuni nctc 11168) product is a highly immunogenic, amino acid-binding protein. cjaa was palmitic acid-modified when processed in e. coli. in addition, site-directed mutagenesis of the cys residue of the laac motif of its signal sequence confirmed that cjaa is a lipoprotein when processed in campylobacter. localization of the protein appeared to be host dependent. in campylobacter, cjaa was recovered mainly as an inner-membran ...200818584243
the haemophilus influenzae hmw1 adhesin is a glycoprotein with an unusual n-linked carbohydrate modification.the haemophilus influenzae hmw1 adhesin mediates adherence to respiratory epithelial cells, a critical early step in the pathogenesis of h. influenzae disease. in recent work, we demonstrated that hmw1 undergoes glycosylation. in addition, we observed that glycosylation of hmw1 is essential for hmw1 tethering to the bacterial surface, a prerequisite for hmw1-mediated adherence to host epithelium. in this study, we examined hmw1 proteolytic fragments by mass spectrometry, achieved 89% amino acid ...200818621734
real-time pcr detection of pathogenic microorganisms in roof-harvested rainwater in southeast queensland, australia.in this study, the microbiological quality of roof-harvested rainwater was assessed by monitoring the concentrations of escherichia coli, enterococci, clostridium perfringens, and bacteroides spp. in rainwater obtained from tanks in southeast queensland, australia. samples were also tested using real-time pcr (with sybr green i dye) for the presence of potential pathogenic microorganisms. of the 27 rainwater samples tested, 17 (63%), 21 (78%), 13 (48%), and 24 (89%) were positive for e. coli, en ...200818621865
detection of campylobacter jejuni and campylobacter coli from broiler chicken-related samples using bax pcr and conventional international organization for standardization culture.in this study, the conventional international organization for standardization (iso) culture method was compared with the dupont qualicon bax system, a high-throughput, rapid molecular assay that can be used to detect several bacterial species, including campylobacter jejuni and campylobacter coli in diverse sample types. standard enrichment culture is a time-consuming process, taking up to 6 days to obtain a confirmed result. rapid molecular assays have been developed that provide results withi ...200818468043
host-pathogen interactions in campylobacter infections: the host perspective.campylobacter is a major cause of acute bacterial diarrhea in humans worldwide. this study was aimed at summarizing the current understanding of host mechanisms involved in the defense against campylobacter by evaluating data available from three sources: (i) epidemiological observations, (ii) observations of patients, and (iii) experimental observations including observations of animal models and human volunteer studies. analysis of available data clearly indicates that an effective immune syst ...200818625685
recombination-based in vivo expression technology identifies helicobacter pylori genes important for host colonization.here we undertook to identify colonization and gastric disease-promoting factors of the human gastric pathogen helicobacter pylori as genes that were induced in response to the stomach environment. using recombination-based in vivo expression technology (rivet), we identified six promoters induced in the host compared to laboratory conditions. three of these promoters, designated pivi10, pivi66, and pivi77, regulate genes that h. pylori may use to interact with other microbes or the host. pivi10 ...200818794279
monoclonal antibody binding to the major outer membrane protein of campylobacter coli.campylobacter species are major enteric pathogens causing diarrhea illness in humans and animals. immunological tests are needed for accurate and rapid identification of c. coli, in conjunction with the use of standard biochemical tests. we initiated the creation of monoclonal antibodies (mabs) using whole c. coli cells as antigen. four positive clones were identified, namely mab2g6, mab3b9, mab4a10 and mab5b9. dot-blot assay and elisa revealed that only mab2g6 did not cross react with c. jejuni ...200818796306
prevalence of thermotolerant campylobacter in pheasants (phasianus colchicus).the present study was undertaken with the aim to evaluate the prevalence of thermotolerant campylobacter spp. in living pheasants in italy. to achieve this goal, a total of 240 living pheasants, equally shared between female and male birds, were examined. thermotolerant campylobacter spp. was isolated in 104 out of 204 (43.3%) living pheasants analysed. campylobacter coli (100%) and campylobacter jejuni (13.5%) were identified by polymerase chain reaction. adult pheasants showed a significantly ...200818798025
identification of campylobacter jejuni proteins recognized by maternal antibodies of chickens.campylobacter jejuni is one of the leading bacterial causes of food-borne gastroenteritis. infection with c. jejuni is frequently acquired through the consumption of undercooked poultry or foods cross-contaminated with raw poultry. given the importance of poultry as a reservoir for campylobacter organisms, investigators have performed studies to understand the protective role of maternal antibodies in the ecology of campylobacter colonization of poultry. in a previous study, chicks with maternal ...200818805999
flagellar glycosylation in clostridium botulinum.flagellins from clostridium botulinum were shown to be post-translationally modified with novel glycan moieties by top-down ms analysis of purified flagellin protein from strains of various toxin serotypes. detailed analyses of flagellin from two strains of c. botulinum demonstrated that the protein is modified by a novel glycan moiety of mass 417 da in o-linkage. bioinformatic analysis of available c. botulinum genomes identified a flagellar glycosylation island containing homologs of genes rec ...200818671733
longitudinal study of prevalence of campylobacter jejuni and campylobacter coli from turkeys and swine grown in close proximity.eastern north carolina is a major contributor to both turkey and swine production in the united states. in this region, turkeys and swine are frequently grown in close proximity and by common growers. to further characterize colonization of turkeys and swine with campylobacter in such a setting, we investigated the prevalence of thermophilic campylobacters in eight paired operations involving turkey farms in close proximity to finishing swine farms. all 15 surveyed flocks and 15 herds were campy ...200818810862
a large waterborne outbreak of campylobacteriosis in norway: the need to focus on distribution system safety.on 7 may 2007 the medical officer in røros (population 5600) reported 15 patients with gastroenteritis. three days later he estimated hundreds being ill. untreated tap water from a groundwater source was suspected as the vehicle and chlorination was started 11 may. campylobacter was isolated from patients' stool samples. we conducted an investigation to identify the source and describe the extent of the outbreak.200818816387
comparative genomic analysis of clinical strains of campylobacter jejuni from south africa.campylobacter jejuni is a common cause of acute gastroenteritis and is also associated with the post-infectious neuropathies, guillain-barré and miller fisher syndromes. in the cape town area of south africa, c. jejuni strains with penner heat-stable (hs) serotype hs:41 have been observed to be overrepresented among cases of guillain-barré syndrome. the present study examined the genetic content of a collection of 32 south african c. jejuni strains with different serotypes, including 13 hs:41 st ...200818431496
antimicrobial resistance of campylobacter spp. isolated from broiler flocks in iceland 2001-2005.minimum inhibitory concentrations of six antimicrobial agents were determined for one campylobacter sp. isolate from each of the 362 campylobacter-positive commercial chicken flocks in iceland in the years 2001-2005. of all isolates tested, 6.9% were resistant, although none were multiresistant. resistance to ampicillin was most commonly observed (3.6%) followed by resistance to enrofloxacin (3%), nalidixic acid (1.9%), and oxytetracyclin (0.3%), with cross-resistance between enrofloxacin and na ...200818321203
the effect of chilling in cold air or ice water on the microbiological quality of broiler carcasses and the population of campylobacter.cold air or ice water can be used to chill poultry carcasses after slaughter. the objective of this study was to compare the effect of 2 chill methods on broiler carcass bacteria. broiler carcasses were cut in half along the dorsal-ventral midline; one half was subjected to an ice-water immersion chill in an agitated bath for 50 min, whereas the reciprocal half was subjected to an air chill in a 1 degrees c cold room for 150 min. total aerobic bacteria, coliforms, escherichia coli, and campyloba ...200818420993
prevalence of gastrointestinal bacterial pathogens in a population of zoo animals.faecal prevalence of gastrointestinal bacterial pathogens, including campylobacter, escherichia coli o157:h7, salmonella, shigella, yersinia, as well as arcobacter, were examined in 317 faecal specimens from 44 animal species in belfast zoological gardens, during july-september 2006. thermophilic campylobacters including campylobacter jejuni, campylobacter coli and campylobacter lari, were the most frequently isolated pathogens, where members of this genus were isolated from 11 animal species (1 ...200818331520
real-time pcr approach for detection of environmental sources of campylobacter strains colonizing broiler flocks.reducing colonization of poultry flocks by campylobacter spp. is a key strategy in the control and prevention human campylobacteriosis. horizontal transmission of campylobacters, from in and around the farm, is the presumed route of flock colonization. however, the identification and prioritization of sources are confounded by the ubiquitous nature of these organisms in the environment, their poor rates of recovery by standard culture methods, and the need for cost-effective and timely methods f ...200818203857
risk factors for infection with campylobacter jejuni flaa genotypes.we aimed to explore campylobacter genotype-specific risk factors in australia. isolates collected prospectively from cases recruited into a case-control study were genotyped using flaa restriction fragment-length polymorphism typing (flaa genotyping). exposure information for cases and controls was collected by telephone interview. risk factors were examined for major flaa genotypes using logistic and multinomial regression. five flaa genotypes accounted for 325 of 590 (55%) cases - flaa-6b (n=1 ...200818205975
campylobacter jejuni survives within epithelial cells by avoiding delivery to lysosomes.campylobacter jejuni is one of the major causes of infectious diarrhea world-wide, although relatively little is know about its mechanisms of pathogenicity. this bacterium can gain entry into intestinal epithelial cells, which is thought to be important for its ability to persistently infect and cause disease. we found that c. jejuni is able to survive within intestinal epithelial cells. however, recovery of intracellular bacteria required pre-culturing under oxygen-limiting conditions, suggesti ...200818225954
the first report on campylobacter coli family outbreak detected in poland in 2006.a family outbreak of gastroenteritis caused by campylobacter coli occurred in may 2006 in bielsko-biala, in the south of poland. four members of a family had non-bloody diarrhea and abdominal cramps. c. coli were isolated in three of the four patients. pfge and pcr-rflp-flaa patterns confirmed the link between cases, showing the usefulness of these methods in outbreak investigation. at the same time, the epidemiological and environmental investigations of this outbreak were very limited and did ...200818445405
comparative performance of different pcr assays for the identification of campylobacter jejuni and campylobacter coli.the sensitivity and specificity of 7 pcr assays described for the identification of campylobacter jejuni and campylobacter coli were examined using alkaline cell lysates from a collection of 100 well characterized reference strains of c. jejuni, c. coli, campylobacter lari and related campylobacter, helicobacter and arcobacter species. based on a preliminary evaluation, one multiplex test was excluded from further evaluation. the various assays differed considerably in sensitivity and specificit ...200818272350
antimicrobial use through feed, water, and injection in 20 swine farms in alberta and saskatchewan.antimicrobial resistance (amr) is an emerging animal welfare and public health issue linked to antimicrobial use (amu) in livestock. this study was conducted in 2004 on 20 swine farms in alberta and saskatchewan. on-farm records and questionnaires were used to retrospectively describe the antimicrobial exposures of pigs through feed, water, and injection. antimicrobial use in all production categories was described over 12 months. on-farm records and questionnaires provided sufficient data to de ...200818505203
performance of a 70-mer oligonucleotide microarray for genotyping of campylobacter jejuni.campylobacter jejuni is widespread in the environment and is the major cause of bacterial gastroenteritis in humans. in the present study we use microarray-based comparative genomic hybridizations (cgh), pulsed-field gel electrophoresis (pfge) and multilocus sequence typing (mlst) to analyze closely related c. jejuni isolates from chicken and human infection.200818462507
comparison of agar dilution and e-test for antimicrobial susceptibility testing of campylobacter coil isolates recovered from 80 ontario swine farms.the primary aim of this study was to evaluate the level of agreement of the e-test for in vitro antimicrobial susceptibility testing of campylobacter coli using the agar dilution technique, which is the approved method. a convenience sample of 80 ontario swine farms was chosen for this study; each farm was visited from january to june 2004. a total of 233 isolates of c. coli were tested for susceptibility to 10 antimicrobials by agar dilution and the e-test. performance of the tests was evaluate ...200818505206
the omp50 gene is transcriptionally controlled by a temperature-dependent mechanism conserved among thermophilic campylobacter species.the thermophilic campylobacters are enteropathogenic for humans. we recently showed that omp50 is a campylobacter species-specific porin produced in campylobacter jejuni and campylobacter lari but not in campylobacter coli. in the present study, we investigated regulation of the omp50 gene and found that its expression in c. jejuni was temperature-dependent, but independent of growth phase or medium viscosity. the use of rt-pcr and omp50::lacz fusions showed that growth temperature control occur ...200818485677
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