PMID(sorted descending)
serogroups of campylobacter jejuni, campylobacter coli, and campylobacter fetus defined by direct immunofluorescence.rabbits were inoculated with whole, formalinized campylobacter jejuni, c. coli, and c. fetus cells; the c. jejuni and c. coli immunogens were identified by their dna relatedness at the species level to the type strains of c. jejuni or c. coli. the designation c. coli was not used among the other c. jejuni strains; they were classified as hippurate-positive or hippurate-negative c. jejuni. immunoglobulin g was isolated from the antisera and labeled with fluorescein isothiocyanate. these conjugate ...19836341405
characterization of tetracycline resistance plasmids from campylobacter jejuni and campylobacter coli.tetracycline resistance in strains of campylobacter jejuni and campylobacter coli was mediated by plasmids. intra- and interspecies transfer was demonstrated within the genus campylobacter. buoyant densities of plasmid dnas ranged from 1.691 to 1.694 g/cm3 (31 to 33% guanine plus cytosine). restriction enzymes acci, bcli, bglii, and psti were found to be most useful for comparing the plasmids. the molecular weight of c. jejuni plasmid pmak175 was 44.7 kilobases (29 x 10(6), and the other plasmid ...19836318666
structural and antigenic heterogeneity of lipopolysaccharides of campylobacter jejuni and campylobacter coli.the techniques of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, silver staining, and immunoblotting were used to analyze the lipopolysaccharide (lps) structure of 20 strains of campylobacter jejuni and 4 strains of campylobacter coli belonging to more than 22 thermostable serotypes. the lpss of all strains examined were shown to be of a low-molecular-weight type, and these low-molecular-weight lpss conferred heat-stable serospecificity. high-molecular-weight banding observed with bo ...19846203838
nucleic acids in the classification of campylobacters.the importance of campylobacters in human disease has stimulated improvements in the methods for identification of strains from hospitals and the environment. reliable and accurate identification depends on a sound classification for which nucleic acid analyses provide fundamental information about species relationships. studies on the genus campylobacter show that the genome dna of species have base compositions of 29 to 38 mol% g + c and molecular weights of 1.54 x 10(9) to 2.31 x 10(9). campy ...19836194994
toxins produced by campylobacter jejuni and campylobacter coli. 19846141372
identification of campylobacter coli isolates from animals and humans by bacterial restriction endonuclease dna analysis.ninety-nine campylobacter coli isolates were examined by bacterial restriction endonuclease dna analysis (brenda) with hindiii. isolates from poultry from the same environment had identical patterns, patterns of isolates carried by suckling piglets were generally the same as those of isolates recovered from their dams, and one human patient yielded the same brenda type when sampled 6 weeks later. the 14 human isolates examined produced 11 distinct brenda types. forty-three c. coli isolates from ...19846093690
[experiments on the electron microscopic differentiation of vibrio fetus, vibrio bubulus, vibrio coli and vibrio metschnikovi]. 19655881830
gnotobiotic pigs exposed to vibrio coli. 19714943837
a method of isolating vibrio coli from pigs. 19694894006
transmission of swine dysentery with treponema hyodysenteriae and vibrio coli. 19744836152
the etiology of swine dysentery. i. oral inoculation of germ-free swine with treponema hyodysenteriae and vibrio coli. 19744466031
a preliminary study on the intestinal flora of cecum and colon of eight, ten and 12 week old swine.using a replica-plating method and differential culture media, members of the enterobacteriaceae and pseudomonaceae were determined quantitatively in the colon and cecum content of pigs from different litters, born on the same farm and kept under the same environmental conditions, from the age of six weeks. the bacterial flora in pigs of eight, ten and 12 weeks of age showed little significant quantitative variation. pigs of 12 weeks of age differed significantly from younger ones with respect t ...19734270808
pathogenicity of vibrio coli for swine. ii. experimental infection of conventional pigs with vibrio coli.the pathogenicity of v. coli for conventional swine was studied by inoculating pigs with cultures of v. coli and v. coli infected gut of gnotobiotic pigs. thus, six conventional pigs were inoculated with strains of v. coli freshly isolated from infected gnotobiotic pigs. the cultures were grown in simulated sows milk, and added to the feed. two other groups, of three pigs each, were infected by administration of minced intestine from two gnotobiotic pigs, heavily infected with the organism. vibr ...19684234783
pathogenicity of vibrio coli for swine. i. experimental infection of gnotobiotic pigs with vibrio coli.fifteen gnotobiotic pigs varying in age from three to eight weeks were exposed to 23 strains of v. coli isolated from swine with clinical and/or pathological signs of swine dysentery and also from clinically healthy pigs. clinical or pathological signs of swine dysentery were not produced, although the organism was readly established in the sedimentary tract. culture of feces, alimentary tract and environment revealed v. coli in large numbers, but no bacterial growth was obtained from other orga ...19684234782
[isolation and identification of vibrio coli and borrelia like germs in the pig's dysentery]. 19724210742
susceptibility of campylobacter jejuni and campylobacter coli to macrolides and related compounds.the susceptibility of 105 thermophilic campylobacters from human and swine origins to eight macrolides and related compounds was tested. erythromycin, josamycin, clindamycin, and ase 136 bs (a new erythromycin derivative) were the most active against the human strains. the swine strains were highly resistant, except to pristinamycin. the human campylobacter coli strains (except for two strains) behaved like the c. jejuni strains.19854091531
[occurrence of 3-amino-3,6-dideoxy-d-glucose in a lipopolysaccharide from campylobacter coli]. 19854087160
inhibition of campylobacter coli and campylobacter jejuni by antibiotics used in selective growth media.the ability of campylobacter coli and campylobacter jejuni to grow in the presence of antibiotics used in selective growth media was compared. mic data for c. coli indicated that some strains were more susceptible to the antibiotics than were the c. jejuni strains tested. a reduction of greater than 1 log cycle in the numbers of cells growing on plates containing antibiotics was considered to be a marked level of inhibition. only one of nine of the antibiotic combinations studied did not markedl ...19854077962
[seasonal distribution of campylobacter jejuni and campylobacter coli (1 september 1982--31 august 1983)]. 19854068889
occurrence of plasmids and antibiotic resistance among campylobacter jejuni and campylobacter coli isolated from healthy and diarrheic animals.serologically defined strains of campylobacter jejuni and campylobacter coli from healthy and diarrheic animals were examined for the occurrence of plasmid dna in association with the antibiotic susceptibility of the bacterial host and the health status of the animal host. of all campylobacter organisms surveyed, 53% (116 of 200) contained plasmid dna. a plasmid occurrence rate of 73.8% was obtained for c. coli from healthy pigs, contrasted by lower plasmid occurrence rates for c. coli from diar ...19854044793
biotypes and serotypes of campylobacter jejuni and campylobacter coli strains isolated from patients, pigs and chickens in the region of rotterdam.a comparison was made of the distribution of the bio-and serotypes of campylobacter jejuni and campylobacter coli isolated from human patients (205 isolates), intestinal contents of pigs (163 isolates) and intestinal contents and livers of chickens (147 isolates). all strains were isolated in the region of rotterdam. campylobacter jejuni biotype 1 accounted for 86%, 1% and 82% of the human, pig and chicken isolates respectively; c. coli was present in 7%, 98% and 14% respectively. serotyping was ...19854031520
enrichment medium for isolation of campylobacter jejuni-campylobacter coli.a broth enrichment medium for the improvement of isolation of campylobacter jejuni-campylobacter coli from stool samples and other specimens is presented. of 1,228 samples examined in parallel, positive results were obtained from 81 by direct inoculation of selective media and from 112 after enrichment. thus, an increase of 27.7% in the isolation rate was obtained by using the enrichment medium. the same medium without antibiotics allows the preservation of isolates of c. jejuni-c. coli for at l ...19854026287
development of a bacteriophage typing system for campylobacter jejuni and campylobacter coli.a bacteriophage typing system for campylobacter jejuni and campylobacter coli was developed with phages isolated from poultry feces. data for phage selection were generated from a set of isolates of c. jejuni and c. coli from humans in illinois. selection of 14 phages from the 47 phages available was assisted by determination of the sneath-jaccard similarity coefficients and subsequent unweighted pair-group arithmetic averaging cluster analysis. the typing set was reproducible and stable in the ...19854019737
serotyping of campylobacter jejuni, campylobacter coli, and campylobacter laridis from domestic and wild using 50 unabsorbed antisera, we were able to serotype 272 (65.7%) of 414 thermotolerant campylobacters from wild and domestic animals, on the basis of heat-stable antigens identified by means of passive hemagglutination. forty-two serotypes were recognized. the pattern of serotypes detected in the various animal species was compared to human clinical isolates by using the czekanowski index (proportional similarity index). the highest degree of similarity to the clinical isolates was observed ...19854015088
antigenicity of lipopolysaccharides from campylobacter jejuni and campylobacter coli in passive haemagglutination tests and enzyme-linked immunosorbent assays.passive haemagglutination tests and elisa were used to study the serological activity of homologous and heterologous rabbit antisera against lps prepared from various strains of c. jejuni/coli. in both test systems the homologous antisera exhibited serological activity against lps. the heterologous antisera showed some degree of intra- and inter- species cross-reactivity. the cross-reacting was most pronounced in the elisa. erythrocytes sensitized with untreated lps gave higher antibody-titres t ...19854013751
cadmium chloride susceptibility, a characteristic of campylobacter spp.we report a simple diagnostic characteristic useful in the presumptive identification of campylobacter jejuni and campylobacter coli. filter paper disks impregnated with cadmium chloride were placed on streaked agar medium. zones of growth inhibition for campylobacter spp. occurred at 1.25 micrograms per disk. other enteropathogens (salmonella spp., shigella spp., vibrio cholerae, vibrio parahaemolyticus, escherichia coli, and yersinia enterocolitica) were resistant to at least 40 micrograms per ...19853998099
typing of heat-stable and heat-labile antigens of campylobacter jejuni and campylobacter coli by coagglutination.a coagglutination system has been devised for typing heat-stable and heat-labile antigens of campylobacter jejuni and c. coli. the use of protein a-positive staphylococcus aureus cells carrying campylobacter sp. serotype antibody and the treatment of campylobacter sp. cells with dnase in the antigen suspension permitted rapid and specific coagglutination of rough (autoagglutinable) as well as smooth cultures. cells of s. aureus were sensitized with campylobacter sp. serotype antisera. four to fi ...19853998098
estimation of campylobacter spp. in broth culture by bioluminescence assay of atp.the luciferin-luciferase bioluminescence reaction was used to estimate cell numbers of campylobacter jejuni and campylobacter coli in broth cultures based on a linear relationship between cell numbers (in excess of 10(4) to 10(5] and atp levels. the sensitivity was lower than that obtained with escherichia coli. the calculated amount of intracellular atp per cell of c. jejuni and c. coli ranged from 1.7 to 2.1 fg.19853994377
[season-related incidence of campylobacter jejuni and campylobacter coli in fecal samples of healthy slaughter cattle]. 19853993275
use of auxotyping for epidemiological studies of campylobacter jejuni and campylobacter coli infections.a chemically defined medium developed for neisseria gonorrhoeae was modified to support the growth of campylobacter jejuni and campylobacter coli. a total of 76 isolates of c. jejuni and 14 isolates of c. coli were tested on this medium, which was designated campylobacter defined medium (cdm), over a 3-month period. although none of the c. coli isolates appeared to require amino acids, 51% of the c. jejuni tested required one and 7% required multiple amino acids for growth. an analysis of isolat ...19853988340
in vitro activities of 47 antimicrobial agents against three campylobacter spp. from pigs.the in vitro activities of 47 antimicrobial agents against 30 isolates of campylobacter species from pigs were determined by the agar dilution technique. the isolates were obtained from pigs with proliferative enteritis and included 10 strains each of campylobacter coli, campylobacter sputorum subsp. mucosalis, and "campylobacter hyointestinalis gebhart et al." (this name is not on the approved lists). carbadox, furazolidone, nitrofurantoin, gentamicin, and dimetridazole were the most active dru ...19853985597
[isolation of campylobacter jejuni and campylobacter coli in fecal samples of healthy slaughter swine depending on the season]. 19853976311
comparison of basal media for culturing campylobacter jejuni and campylobacter coli.four strains of campylobacter jejuni and four strains of campylobacter coli were used to compare the quantitative growth of campylobacter cells on blood agar base no. 2 (oxoid), brucella agar (bbl microbiology systems and difco laboratories), campylobacter agar base (difco), columbia blood agar base (difco and oxoid), and mueller-hinton agar (difco and oxoid). columbia blood agar base and blood agar base no. 2 were inhibitory to most of the strains tested, as evidenced by reduced (10- to 1,000-f ...19853972990
susceptibility of campylobacter isolates to the bactericidal activity of human serum.although campylobacter jejuni and related thermophilic organisms are more common human pathogens than are campylobacter fetus, most bloodstream or systemic isolates are c. fetus. to understand the pathophysiology related to this observation, the authors studied susceptibility to the bactericidal activity of normal human serum of campylobacter coli, c. jejuni, and c. fetus isolates from feces and blood. in standardized assays, 10 of 15 c. jejuni and related isolates showed 90% kill (mean, 90.6% + ...19853968449
distribution and serotypes of campylobacter jejuni and campylobacter coli in enteric campylobacter strains isolated from children in the central african hundred eighty-five enteric campylobacter strains isolated from diarrheic or healthy children in bangui (central african republic) were studied to determine their species and serotypes. c. coli was identified in 38.9% of all strains and in 43.9% of strains from diarrheic children. by the hemagglutination technique for heat-stable antigens, 73.5% of the strains could be serotyped. of the typeable strains, 75% were distributed among 13 more frequent serotypes. c. coli serotype pen 37,56 was th ...19863958146
in vivo transfer of genetic information between gram-positive and gram-negative bacteria.a 1427-bp dna fragment containing the kanamycin resistance gene, apha-3, of plasmid pip1433 from campylobacter coli was inserted into a shuttle vector. full expression of apha-3 was obtained in bacillus subtilis and in escherichia coli. this dna fragment was sequenced in its entirety and the starting point for apha-3 transcription in b. subtilis, c. coli and e. coli was determined by s1 nuclease mapping. the sequence of the promoter consists of the hexanucleotides ttgaca and tataat, with a spaci ...19853937729
[isolation of campylobacter jejuni and campylobacter coli from intestinal contents and feces of chickens]. 19853925041
in vitro blastogenic response and immunoglobulin synthesis in murine spleen cells stimulated with lipopolysaccharides from campylobacter jejuni and campylobacter coli.lipopolysaccharides isolated from strains of campylobacter jejuni and campylobacter coli were mitogenic for spleen cells as measured by 3h-thymidine incorporation. the incorporation was dose dependent with an increase with increased concentration of lps. addition of lps beyond 500 micrograms/ml gave inhibition of incorporation. stimulation of the spleen cells for 3 days with lps led to a polyclonal activation of immunoglobulin synthesis. the amount of immunoglobulins synthesised showed a maximum ...19853909739
isolation, characterization, and serotyping of campylobacter jejuni and campylobacter coli from slaughter cattle.a total of 525 specimens from 100 slaughter beef cattle were examined for the presence of campylobacter jejuni and campylobacter coli by direct plating and enrichment techniques. isolates were identified by cultural, biochemical, antibiotic sensitivity, and immunofluorescence tests and further characterized with the aid of recently developed biotyping and serotyping methods. fifty animals were positive for c. jejuni; only one was positive for c. coli. the distribution pattern of c. jejuni-positi ...19853888109
comparative in vitro activities of twelve antimicrobial agents against campylobacter species.the in vitro susceptibility of 27 campylobacter jejuni, 31 campylobacter coli, and 30 campylobacter fetus subsp. fetus strains to 12 antimicrobial agents was determined. ciprofloxacin, a new quinoline derivative, was the most active agent tested. antimicrobial susceptibility differed among the three species tested.19853873216
[epidemiology and epizootiology of enteritis caused by campylobacter jejuni and campylobacter coli]. 19863822876
demonstration of a flagellar antigen shared by a diverse group of spiral-shaped bacteria that colonize intestinal mucus.western blot analysis showed that there is little immunological cross-reactivity of the human pathogens campylobacter jejuni and campylobacter coli with campylobacter pyloridis, except for a very strong cross-reaction between the flagellins. this same antigenic cross-reaction was found with two isolates of gram-negative spiral microaerophilic bacteria that are known to colonize the intestinal mucosa of rodents, but not with the flagellins of a number of other motile bacteria. it is proposed that ...19873818099
monosaccharide composition of lipopolysaccharides from campylobacter jejuni and campylobacter coli.the monosaccharide composition of the lps from 5 campylobacter jejuni strains and 7 campylobacter coli strains has been studied. all lps's contained kdo, heptose, glucosamine, glucose, and (with one exception) galactose. all c. jejuni and 3 c. coli lps's contained greater than 1% galactosamine. 3-amino-3.6-dideoxyglucose was present in all but one c. coli lps and in only one c. jejuni lps.19863772734
rate of campylobacter spp. isolation in three regions of ontario, canada, from 1978 to 1985.isolation rates of campylobacter spp. were analyzed for an 8-year period, 1978 through 1985. three laboratories of the ontario ministry of health examined 146,842 human feces samples for bacterial pathogens, including campylobacter jejuni and campylobacter coli. campylobacter spp. were isolated from 5,580 specimens (3.8%), with monthly isolation rates ranging from 1.1 to 7.4%. the data showed a seasonal distribution of isolations, with peaks during the summer months (june to september). most inf ...19863771774
biotype and serogroup distribution of campylobacter isolates from children in nigeria.a total of 101 campylobacter isolates from nigerian children with or without gastroenteritis were biotyped and serogrouped by using the lior typing schemes (h. lior, j. clin. microbiol. 20:636-640, 1984; h. lior, d. l. woodward, j. a. edgar, l. j. laroche, and p. gill, j. clin. microbiol. 15:761-768, 1982). fifty-three (52.5%) of the isolates were campylobacter jejuni biotype i, 29 (28.7%) were c. jejuni biotype ii, 10 (9.9%) were campylobacter coli biotype i, and 9 (8.9%) were c. coli biotype i ...19863771772
modified selective medium for isolation of campylobacter spp. from feces: comparison with preston medium, a blood-free medium, and a filtration system.our previously described (h. goossens, m. de boeck, and j. p. butzler, eur. j. clin. microbiol. 2:389-393, 1983) selective medium, consisting of cefoperazone (15 mg/liter), rifampin (10 mg/liter), colistin (10,000 iu/liter), and amphotericin b (2 mg/liter) (medium m1), for the isolation of campylobacter jejuni and campylobacter coli from stool specimens was modified as follows: cefoperazone (30 mg/liter), rifampin (10 mg/liter), and amphotericin b (2 mg/liter) (medium m2). a comparative study of ...19863771769
isolation and characterization of cephalothin-susceptible campylobacter coli from slaughter a recent meat survey, 10 of 13 (77%) campylobacter coli isolates were susceptible to cephalothin. these organisms were isolated from nine slaughter cattle from eight meat packing establishments. all 10 isolates grew at 43 degrees c but not at 25 degrees c, were catalase and oxidase positive, and were susceptible to nalidixic acid (30 micrograms) and cephalothin (30 micrograms). the cultures were subsequently identified as c. coli serogroup 20, biotype i (lior scheme). sodium dodecyl sulfate-p ...19863771747
association with hela cells of campylobacter jejuni and campylobacter coli isolated from human feces.we developed a rapid in vitro test for determining the association of campylobacter jejuni and c. coli with hela cells. association was expressed as a weighted mean of the number of bacteria associated with one cell in an association index (ai). the reproducibility of the ai was checked by repeating the test six times, using four strains chosen at random. means and standard deviations of the means were 7.3 +/- 1.2, 6.8 +/- 0.9, 1.8 +/- 1.2, and 0.1 +/- 0.2. the experimental conditions for which ...19863770943
the chicken embryo as a model for campylobacter invasion: comparative virulence of human isolates of campylobacter jejuni and campylobacter coli.eleven-day-old chicken embryos were used to compare the relative virulence of minimally passaged human isolates of campylobacter jejuni and campylobacter coli. graded doses of bacteria were inoculated onto the chorioallantoic membrane, and 50% lethal doses were calculated at 72 h postinfection. strains varied markedly in their ability to invade the chorioallantoic membrane and kill the embryos. the 50% lethal doses varied by about 6 logs for 25 strains of c. jejuni, and by 2 logs for 5 strains o ...19863759232
strain characterization and grouping of campylobacter jejuni and campylobacter coli by interaction with lectins.strains of campylobacter jejuni and campylobacter coli were characterized and grouped by their distinct reaction patterns with lectins. heating of the campylobacter cultures to 100 degrees c and holding for 30 to 60 min greatly enhanced their reactivity with lectins and permitted the grouping of all but 3 of 155 cultures tested in this study without interference of autoagglutination and other nonspecific activities. the lectin reaction patterns of the heated cultures were stable and reproducible ...19863754264
[waste water from poultry coops, a potential source of human infection with campylobacter coli]. 19863749726
campylobacter colitis in ranch mink in ontario.outbreaks of colitis, where campylobacter jejuni and campylobacter coli were the only pathogens isolated occurred in weanling mink (mustella vision) on two commercial mink ranches in ontario. lesions were restricted to the proximal colon and were characterized by multiple 1 mm focal or 1 mm linear erosions/ulcers in the region 2 cm distal to the ileal-colonic junction. histological changes included thickening of the colonic mucosa, inflammatory cell infiltrate in the lamina propria and submucosa ...19863742356
effect of iron concentration on toxin production in campylobacter jejuni and campylobacter coli.the effect of iron concentrations in culture media on supernatant yields of campylobacter cytotonic toxin (cct) was studied. of the 118 campylobacter spp. strains surveyed, 78.8% produced toxin in brucella broth or in casamino acids--yeast extract (cye) broth. when the iron concentration of cye was increased from 0.44 microgram/ml (7.9 microm) to 0.65 microgram/ml (11.6 microm) by the addition of ferric chloride, 94.9% of the strains were positive for toxin in a ganglioside gm1 based, enzyme-lin ...19863719459
bacteria in enteric lesions of horses.thirty-three species of bacteria were isolated from the gastrointestinal mucosa of 23 adult horses and two foals. the bacteria isolated could be related to gross and microscopical lesions in some cases. clostridium perfringens type a, actinobacillus equuli, salmonella typhimurium and campylobacter coli biotype 1 could all be associated with gastrointestinal lesions. c jejuni biotype 1 and aeromonas hydrophila were both recovered in this study and have been identified as causes of enteritis in ho ...19863716108
rapid and improved gas-liquid chromatography technique for detection of hippurate hydrolysis by campylobacter jejuni and campylobacter coli.a gas-liquid chromatographic method which requires no chloroform extraction of the split products has been investigated for the detection of hippurate hydrolysis by campylobacter spp. this technique gave better reproducibility than other tests also used in this study and allows the routine use of the gas-liquid chromatographic method for identification of campylobacter isolates.19873654950
naturally occurring auxotrophs of campylobacter jejuni and campylobacter coli.the nutritional requirements for 439 campylobacter jejuni isolates and 46 campylobacter coli isolates were determined by using a previously described chemically defined medium, campylobacter defined medium. with this medium, 45% of both human and nonhuman c. jejuni isolates demonstrated auxotrophic requirements. none of the 46 c. coli isolates studied demonstrated requirements for amino acids on campylobacter defined medium. the most common auxotrophic requirement among c. jejuni isolates was fo ...19873654939
evaluation of gamma radiation levels for reducing pathogenic bacteria and fungi in animal sewage and laboratory effluents.sewage samples collected from animal wastes and from effluents at an animal disease laboratory were inoculated with known numbers of pathogenic organisms and subjected to various doses of gamma radiation from a 60co source. surviving test organisms were quantitatively determined by selective and enrichment techniques. the experiment was modeled as a quantal assay in which probit analysis was applied to obtain d10 values. the d10 value represents the irradiating dose required to reduce the popula ...19873651881
in vitro susceptibility of campylobacter jejuni and campylobacter coli isolated in denmark to fourteen antimicrobial agents.the in vitro susceptibility of 95 recent danish human clinical isolates of campylobacter jejuni and campylobacter coli to 14 antimicrobial agents was determined by an agar dilution technique. c. jejuni and c. coli were considered together. doxycycline, ciprofloxacin, gentamicin, clindamycin, nitrofurantoin and erythromycin were the most active drugs. 2% of the strains tested were resistant to erythromycin (mic greater than 3.1 micrograms/ml). therefore susceptibility testing is suggested if anti ...19873630712
campylobacter jejuni/campylobacter coli-associated guillain-barré syndrome. immunoblot confirmation of the serological response.immunoblot (western blot) examination of the sera of 45 patients with the guillain-barré syndrome demonstrated that between three and five immunoreactive bands that were characteristic of a recent campylobacter jejuni/campylobacter coli infection were present in 22 of these patients. the immunoblots paralleled the serological response that is found in campylobacter enteritis and confirmed the specificity of our previous enzyme-linked immunosorbent assays (elisas), which indicates that antecedent ...19873626926
utilization of monoclonal antibodies to evaluate the involvement of campylobacter jejuni in proliferative ileitis in syrian hamsters (mesocricetis auratus).the role of campylobacter jejuni in the pathogenesis of proliferative ileitis in syrian hamsters was evaluated with monoclonal antibodies of different specificities. monoclonal antibodies were produced with two different specificities: one for all members of the genus campylobacter tested (antibody 8322-2e6) and one for c. jejuni and campylobacter coli (antibodies 841-2a11, 841-4c6, and 841-5b1). heal sections from healthy hamsters, from hamsters with naturally occurring proliferative ileitis, a ...19873623701
effect of clavulanic acid on susceptibility of campylobacter jejuni and campylobacter coli to eight beta-lactam antibiotics.the effect of clavulanic acid on the susceptibility of 32 strains of campylobacter jejuni and campylobacter coli to eight beta-lactam agents was studied. almost all strains tested became susceptible to amoxicillin and ticarcillin with 1 microgram of clavulanic acid per ml. this compound had little or no effect on susceptibility to penicillin g, cephalothin, cefamandole, and cefoxitin. clavulanic acid had a marginal effect on cefotaxime and moxalactam susceptibility.19873619428
atypical campylobacters associated with gastroenteritis.nine strains of campylobacter species other than campylobacter jejuni, campylobacter coli, and campylobacter laridis were isolated from patients with acute diarrhea. all nine strains showed preferred growth at 37 degrees c under microaerophilic conditions. conventional microbiological tests and dna-dna dot blotting were used to identify these strains. three of the nine campylobacter strains hydrolyzed hippurate, reduced nitrate, produced catalase, were resistant to cephalothin, and were shown to ...19873611317
[the epidemiology of campylobacter jejuni and campylobacter coli in the fattening chicken]. 19873589638
seasonal occurrence of campylobacter spp. in surface waters and their correlation with standard indicator bacteria.campylobacter jejuni, campylobacter coli, and a campylobacter-like organism were isolated from a number of natural water sources in central washington, including ponds, lakes, and small mountain streams at elevations ranging from 1,460 to 5,400 feet (ca. 445 to 1,646 m) above sea level. at the two sites where extensive sampling was done, the bacteria were recovered throughout the year. generally, the recovery rates were highest in the fall and winter months and lowest during the spring and summe ...19873579268
erythromycin-resistant campylobacter infections in thailand.erythromycin therapy was compared with no treatment in a prospective trial of acute diarrheal disease among 100 infants in an orphanage in bangkok. within 24 h of the onset of diarrhea, 50 children received erythromycin ethylsuccinate (40 mg/kg per day) in four divided doses for 5 days. campylobacter jejuni isolated from 31, campylobacter coli isolated from 21, and shigella spp. isolated from 21 of 100 children were the most commonly recognized pathogens; use of a sensitive, nonselective method ...19873579261
prevalence of campylobacter jejuni and campylobacter coli among wild and domestic animals in yamaguchi prefecture. 19863574158
spontaneous bacterial peritonitis due to campylobacter coli.a patient with alcoholic liver disease and ascites who developed fatal spontaneous bacterial peritonitis due to campylobacter coli is reported herein. this is the first case report of spontaneous bacterial peritonitis due to this organism. campylobacter infections and their relationship to spontaneous bacterial peritonitis are reviewed.19873569775
a study of factors affecting the sensitivity of the passive haemagglutination method for serotyping campylobacter jejuni and campylobacter coli and recommendations for a more rapid procedure.factors affecting the sensitivity of the passive haemagglutination method for serotyping campylobacters have been studied. the concentration of red blood cells during the haemagglutination stage of the procedure markedly affected the titer obtained. an increase in concentration of red blood cells resulted in a lower titer, with titers being inversely proportional to red blood cell concentration. no differences in titer were observed when erythrocytes were sensitized at a range of ph values betwe ...19873567742
[experimental animal studies with campylobacter jejuni and campylobacter coli in chickens]. 19873566434
methods for detecting and enumerating campylobacter jejuni and campylobacter coli in poultry.methods, media, and biochemical tests for detecting, enumerating, isolating, and identifying campylobacter jejuni and c. coli in foods are summarized with special consideration of poultry and poultry products. information is drawn largely from the american public health association compendium of methods for the microbiological examination of foods and the us food and drug administration bacteriological analytical manual for foods. reference is also made to recently advanced techniques and proced ...19863554203
abortion and perinatal sepsis associated with campylobacter infection.fetal loss or neonatal sepsis associated with campylobacter infection during pregnancy is infrequently recognized. as reported herein, one case of premature labor and neonatal sepsis due to campylobacter fetus subspecies fetus was treated successfully with ampicillin and gentamicin. only 19 similar cases have been cited in the literature. a review of these 19 cases reveals that the campylobacter species involved were probably c. fetus subspecies fetus in nine instances, campylobacter jejuni in n ...19863523697
two-dimensional thin-layer chromatography for the specific detection of hippurate hydrolysis by microorganisms.glycine, one of the end products of hippurate hydrolysis by microorganisms, was detected by a rapid, specific technique utilizing two-dimensional thin-layer chromatography. a loopful of growth of each organism from its suitable agar medium was washed, suspended, and incubated with 0.1% sodium hippurate for 30 min at 37 degrees c. the supernatant of the incubated suspension from each organism was then dansylated, and the dansyl derivatives were separated by two-dimensional thin-layer chromatograp ...19863517036
western blot analysis of intestinal secretory immunoglobulin a response to campylobacter jejuni antigens in patients with naturally acquired campylobacter enteritis.secretory immunoglobulin a (siga) response at the intestinal mucosa is a primary defense against enteric infections. we sought to determine which antigens of campylobacter jejuni outer membranes elicited siga responses in 8 patients with naturally acquired campylobacter enteritis using western blot analysis of fecal extracts. naturally acquired campylobacter infection elicited an siga response in 7 of 8 patients. of these 7 patients, 5 had campylobacter-specific siga titers of 1:16 and two had t ...19863514359
extraintestinal campylobacter jejuni and campylobacter coli infections: host factors and strain determine whether extraintestinal isolates of campylobacter jejuni and campylobacter coli are the consequence of unusual host or bacterial characteristics, we studied clinical and bacteriologic features of 24 extraintestinal infections. common serotypes and auxotypes were present among the extraintestinal isolates. gastrointestinal isolates were more susceptible to normal human serum than were the systemic isolates; however, the ranges overlapped considerably. predispositions to systemic spre ...19863512731
[the survival of campylobacter coli in raw milk].the survival of three strains of campylobacter coli (two isolated from milk of a cow with mastitis, one from poultry intestine) was studied in unpasteurized milk. survival of c. coli strains at 37 degrees c varied greatly: bovine strains showed a 1-log10 decrease in viable cells, and the poultry strain showed 5-log10 decrease after 48 hours. bovine strains lived longer (5 days) than poultry strain (2 days) at 37 degrees c. there was no difference between the strains from different origin in unpa ...19873503963
high frequency of isolation of campylobacter coli from poultry meat in france. 19873440462
serogroups and biotypes of human strains of campylobacter jejuni and campylobacter coli isolated in france.the lior schemes were used for biotyping and serogrouping campylobacter strains isolated during a three year period in bordeaux, france. of the 226 strains tested, 176 were campylobacter jejuni and 50 campylobacter coli. campylobacter jejuni biotype i was the most prevalent (48.2%). biotypes iii and iv of campylobacter jejuni were rare (3.9% and 1.3% respectively). serogroup 4 (17.7%) was the most common serogroup followed by serogroups 46 (11.0%), 29 (10.1%), 9 and 36 (7.9%). eight serogroups c ...19873440456
susceptibility of campylobacter species to metronidazole, its bioactive metabolites and tinidazole.the susceptibilities of 115 campylobacters, belonging to five species, to metronidazole, its principal oxidative metabolites and tinidazole were determined by an agar dilution method. the activities of metronidazole and tinidazole were comparable. the hydroxy metabolite was more active than the parent drug against most species, particularly campylobacter jejuni and campylobacter pyloridis. although the acid derivative was less active against c. jejuni and campylobacter coli, it demonstrated an e ...19873436679
dna probes for identification of tetracycline resistance genes in campylobacter species isolated from swine and cattle.tetracycline-resistant strains of campylobacter jejuni and campylobacter coli from swine and cattle colons were isolated and characterized by hybridization with dna probes. a probe consisting of the 1.8-kilobase (kb) hincii fragment from pua466 was highly specific for the detection of tetracycline resistance (tcr) in c. jejuni and c. coli. the 5-kb tetm dna probe from streptococcus agalactiae plasmid pji3 which has homology with the 1.8-kb hincii fragment from pua466 could also be used to detect ...19873435114
antibody response to campylobacter coli in children during intestinal infection and the central african republic, etiological studies of diarrhea have shown that campylobacter coli accounts for almost 40% of campylobacter enteric isolations. this prompted us to investigate the antibody response to c. coli infection in children. as expected from the literature on campylobacter jejuni infections, our results show that both infection and carriage elicited antibodies against glycine-extracted membrane antigens, flagella, and cholera toxin. the human antibody response to c. coli ...19883410956
[animal experiments with campylobacter coli using suckling pigs]. 19883395182
serovars and biovars of campylobacter strains isolated from humans and slaughterhouse animals in northern germany.a total of 318 campylobacter strains from sporadic cases of human enteritis (109 strains) and healthy slaughterhouse animals in northern germany (209 strains) were bio- and serotyped according to the lior typing schemes. three hundred strains were typable (94.3%) and 38 serovars were identified. among human strains 28 serovars were identified with 30% of them belonging to serovar 4. strains from pigs were associated with 25 serovars, the most frequent being serovar 20 (21.2%). fourteen serovars ...19883372398
characterization of freshly isolated campylobacter coli strains and suitability of selective media for their growth.typical and atypical campylobacter strains were isolated from the colons of cattle and swine by techniques that enabled the selective pressures of antibiotics to be avoided. some cephalothin-susceptible strains and a strain with an indeterminate hippurate reaction were classified as campylobacter coli by dna homology testing. tetracycline-resistant isolates were obtained from animals with no recorded exposure to antibiotics. a selection of 12 c. coli and 6 c. jejuni strains was used to determine ...19883356790
ovine abortion associated with campylobacter coli. 19883354164
characterization of an unclassified microaerophilic bacterium associated with gastroenteritis.four isolates of an unclassified microaerophilic bacterium resembling campylobacter species were characterized by growth requirements, microscopic examination, biochemical characteristics, antimicrobial susceptibility tests, and protein profile analysis. the unclassified isolates were differentiated from campylobacter jejuni, campylobacter coli, campylobacter fetus subsp. fetus, campylobacter laridis, campylobacter pylori, and an ovine isolate. the bacterium was fusiform shaped with a corrugated ...19883343302
expression of campylobacter jejuni invasiveness in cell cultures coinfected with other bacteria.enteroinvasive salmonella, shigella, and escherichia coli strains were found to exert an effect which rendered campylobacter jejuni capable of intracellular localization in epithelial cells in vitro. when monolayers of hep-2 or a-549 cells were challenged with pure cultures of c. jejuni or campylobacter coli, none of the eight strains tested invaded the cells. in contrast, four of these strains were able to localize intracellularly when the cells were challenged with a mixture of campylobacters ...19873312015
small rodents and other mammals associated with mountain meadows as reservoirs of giardia spp. and campylobacter spp.sixty-five percent (469 of 722) of the fecal samples collected from small rodents in the central washington cascade mountains were positive for giardia spp. trapping studies showed that microtines of the genus microtus were heavily infected with the parasite. morphologically the cysts and trophozoites were of the giardia duodenalis type. small-rodent populations appear to maintain their infection throughout the year. our data suggest that there is no difference in the percentage of positive anim ...19873310881
acid and alkaline campylobacter extracts in the demonstration of antibody response in hyperimmunized rabbits.acid and alkaline extracts from several campylobacter strains were used as antigens in enzyme immunoassay (eia). antibodies were detected in rabbits immunized with formalin-treated, boiled, or autoclaved whole bacteria of different campylobacter subspecies. for the preparation of the extracts, three campylobacter fetus subsp. venerealis strains, three campylobacter fetus subsp. fetus strains, four campylobacter coli, and five campylobacter jejuni strains were used. the acid extracts of campyloba ...19873296648
the response of campylobacter jejuni and campylobacter coli in the sydney rock oyster (crassostrea commercialis), during depuration and rock oysters, when allowed to feed in waters containing approximately 10(4) cfu of campylobacter cells per ml, concentrated between 10(2) and 10(3) cfu of the organism per g of oyster tissue, within 1 h. when these contaminated oysters were subjected to depuration, they were effectively cleaned in 48 h. the survival of campylobacter jejuni and campylobacter coli was also investigated. oysters contaminated by feeding and injection were processed as half shells and bottled oysters and were ...19883275320
[campylobacter jejuni and campylobacter coli: epidemiologic markers of isolated strains in santiago, chile]. 19883267894
prevalence of campylobacter in infant, juvenile and adult laboratory primates.a cross-sectional study of campylobacter spp. infection was conducted on 125 infant (macaca nemestrina and macaca fascicularis) in an infant primate nursery housing infants from birth to 18 months of age, and 145 m. nemestrina aged from 4 months to 15 years at another facility (primate field station) housing animals from birth to aged adults. the objective was to determine the prevalence of campylobacter spp. in various age groups and to investigate the correlation with diarrhea. in the infant p ...19883265462
antimicrobial susceptibility of campylobacter jejuni, campylobacter coli, and campylobacter laridis from cats, dogs, pigs, and seagulls. 19883210481
detection of two different kanamycin resistance genes in naturally occurring isolates of campylobacter jejuni and campylobacter coli.a total of 225 isolates of campylobacter jejuni and 54 isolates of campylobacter coli were screened for resistance to kanamycin. among these, five resistant isolates of c. jejuni and six resistant isolates of c. coli, all with different plasmid patterns, were identified. each contained at least one plasmid greater than or equal to 41 kilobases in size. the mic of kanamycin for all 11 strains was determined to be greater than or equal to 256 micrograms/ml by an agar dilution method. in addition, ...19883190204
infection with campylobacter coli and campylobacter fetus in a patient on continuous ambulatory peritoneal dialysis. 19883183412
the in-vitro activity of pd127,391, a new quinolone.mics of pd127,391 a new 4-quinolone, and of ci934 and ciprofloxacin, two previously reported 4-quinolones, were determined for common clinical bacterial isolates by an agar-dilution method. pd127,391 was the most active drug against enterobacteriaceae and acinetobacter spp (mics less than 0.12 mg/l) and as active as ciprofloxacin against aeromonas spp. (mics less than 0.008 mg/l) and pseudomonas aeruginosa (mics less than 1 mg/l). it was more active than ciprofloxacin against pseudomonas spp. in ...19883182416
a specific dna probe for the identification of campylobacter jejuni.a 6.1 kb dna probe for the human enteric pathogen campylobacter jejuni has been isolated from a genomic library constructed in the plasmid vector pbr322 in escherichia coli. the dna sequence used as a probe was identified from recombinant plasmids following immunological screening of transformants using polyclonal antisera to whole cells and to membrane antigens of c. jejuni. restriction endonuclease fragment mapping of c. jejuni dna inserts from three of the recombinant plasmids showed an overl ...19883171548
[survey for the contamination of campylobacter jejuni and campylobacter coli among raw meat and the environment of poultry processing plants]. 19883137280
reassessment of selective agars and filtration techniques for isolation of campylobacter species from faeces.four different studies were conducted in order to re-evaluate conventional methods and assess the efficacy of new selective agars and a filtration method for the isolation of campylobacters. skirrow's medium, preston agar, modified ccd agar and fennell's medium, incubated microaerobically at 37 degrees c for 48 h, gave similar campylobacter isolation rates from 225 faecal samples, but the latter two media were more selective. evaluation of modified ccd agar demonstrated that campylobacters could ...19883134202
[quantitative and qualitative studies of campylobacter in a sewage treatment plant].the occurrence and distribution of campylobacter spp. in the wastewater of a sewage treatment plant was examined from july 1985 to july 1986. the number of campylobacter was determined in a double set-up by the mpn-method. preston broth and -agar containing fbp supplement were used as nutrient media. the samples were incubated at 37 degrees c and 42 degrees c, respectively. subsequently 1345 isolated campylobacter strains were biotyped. the number of fecal and total coliforms, ph-value and tempe ...19883131984
[quantitative and qualitative studies of campylobacter in the sewage of a large city].in a one-year-study wastewater from the sewage system of a big town was examined for the occurrence and distribution of campylobacter spp. the sewage consisted mainly of domestic and industrial wastewater, but also to a small degree of agricultural wastewater and abattoir effluent. preston broth and agar were used as culture media. the number of campylobacter was determined parallel at 37 degrees c and 42 degrees c by the mpn-method. three colonies of each agar plate were isolated and biotyped. ...19883131983
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