molecular characterization of kata from campylobacter jejuni and generation of a catalase-deficient mutant of campylobacter coli by interspecific allelic exchange.a gene encoding catalase (hydrogen-peroxide:hydrogen-peroxide oxidoreductase; ec from campylobacter jejuni was cloned by functional complementation of a catalase-deficient mutant of escherichia coli. the catalase structural gene, designated kata, was assigned by subcloning and its nucleotide sequence determined. the deduced protein product of 508 amino acids, which had a calculated molecular mass of 58,346 da, was found to be structurally and enzymically similar to hydrogen-peroxidases ...19957670638
common somatic o and heat-labile serotypes among campylobacter strains from sporadic infections in the united states.somatic o (formerly heat-stable) and heat-labile (hl) serotyping methods are commonly used to type campylobacter jejuni and campylobacter coli isolates. although both systems are effective, the labor and time required for each have limited their application. these systems can be simplified by reducing the number of antisera used. to find an appropriate panel of antisera, we determined the distribution of common serotypes in the united states among a representative sample of 298 campylobacter iso ...19937686183
cross-reactive antigens shared by pseudomonas aeruginosa, helicobacter pylori, campylobacter jejuni, and haemophilus influenzae may cause false-positive titers of antibody to h. pylori.cystic fibrosis (cf) patients suffer from many of the gastrointestinal conditions which occur in non-cf individuals, e.g., dyspepsia and peptic ulceration. these symptoms may be caused by helicobacter pylori but could also be due to either pancreatic insufficiency or the intensive antibiotic treatment used in cf patients. since cf patients chronically infected with pseudomonas aeruginosa produce antibodies against a wide range of antigens, including antigens common to many other bacteria, e.g., ...19957697522
occurrence of plasmids and tetracycline resistance among campylobacter jejuni and campylobacter coli isolated from whole market chickens and clinical samples.twenty whole market chickens, purchased from 10 different stores in the taipei metropolitan area, were examined for the presence of campylobacter jejuni and campylobacter coli. the microorganisms were recovered from 95% of the chickens. a survey of different sites on--breast, thigh and tail--showed that contamination was equally common on all these sites. one hundred and sixty-seven chicken isolates and the 41 clinical isolates of campylobacter jejuni were examined for the occurrence of plasmid ...19947703010
[diarrheal diseases in 1 to 4 week old suckling piglets from problem herds: microbial spectrum, histology, enzyme histochemistry].postmortem investigation was performed on 54 scouring (soon after onset of diarrhea) and 23 healthy suckling piglets. the animals were 1 to 4 weeks of age and were collected from 27 herds. in 21 herds diarrhea had been observed for a long time in piglets in this age group. coccidia were found in 34 piglets, rotavirus in 2, cryptosporidia in 2, enterotoxigenic e. coli in 6, attaching and effacing e. coli in 1, cl. perfringens type c in 1, campylobacter coli in 53 and c. hyointestinalis in 8 pigle ...19947709191
expression and characterization of campylobacter jejuni benzoylglycine amidohydrolase (hippuricase) gene in escherichia coli.the basis for the difference between campylobacter jejuni and campylobacter coli is the presence and expression of the n-benzoylglycine amidohydrolase (hippuricase) gene only in c. jejuni. a pbr322 recombinant clone (phip-o) of c. jejuni tgh9011 capable of converting hippuric acid into benzoic acid and glycine, the hallmark of hippuricase activity, was characterized and sequenced. the hippuricase gene (hipo) was identified by use of deletion subclones and insertional inactivation. the transcript ...19957730270
a pcr assay for the detection of campylobacter jejuni and campylobacter coli in water.a pcr assay has been developed for the detection of campylobacter jejuni and camp. coli in water samples. the sample is filtered through a membrane which is subjected to sonication to release the impacted cells. after removal of the filter from the cell suspension and a freeze/thaw cell lysis step, a semi-nested pcr is carried out on the filtrate using the primers cf02, cf03 and cf04 (camp. jejuni flaa and flab gene sequences). incorporation of a sonication stage allows removal of the filter mem ...19947765441
incidence and transmission of antibiotic resistance in campylobacter jejuni and campylobacter and two campylobacter clinical isolates were characterized for their in-vitro resistance to erythromycin (1.9%), three fluoroquinolones (31.3-34.3%), tetracycline (43.1%), kanamycin (4.8%), ampicillin (18.6%) and other 16 antimicrobial agents. conjugative transfer of tetracycline and kanamycin resistances among these strains was achieved and small plasmids of 4.3, 4 and 1.9 kb were observed in kanamycin-resistant campylobacter coli strains.19957768766
investigation of helicobacter pylori ascorbic acid oxidating activity.helicobacter pylori sonicate was shown to oxidize ascorbic acid. ascorbic acid oxidation was determined by chromatography combined with electrochemical detection. water soluble ascorbic acid oxidase activity was rather independent of ph with a ph optimum around 2. by gel filtration the oxidizing activity co-eluted with an absorbency peak at 408 nm. the relative molecular mass (mr) was approximately 14,000. it is suggested that this oxidating activity was caused by a cytochrome c-like molecule. a ...19957773246
restriction fragment length polymorphisms among the flagellar genes of the lior heat-labile serogroup reference strains and field strains of campylobacter jejuni and c. coli.several typing systems have been described for campylobacter jejuni and c. coli, to assess the complex epidemiology of these important enteric pathogens. in the present study two typing methods, slide agglutination according to the lior scheme, and the demonstration of restriction-fragment length polymorphisms (rflp) of flagellar genes, have been used in parallel on a set of 194 strains. this set comprised 118 sero-reference strains of c. jejuni and c. coli of the lior scheme, as well as 76 clin ...19957781730
identification of campylobacter jejuni on the basis of a species-specific gene that encodes a membrane facilitate discrimination between the closely related enteropathogens campylobacter jejuni and c. coli, unique differences in antigenic surface structure were examined. a genomic library of c. jejuni 81116 was constructed in plasmid pbluescriptiisk- and expressed in escherichia coli k-12. rabbit hyperimmune serum raised against c. jejuni atcc 29428 recognized a clone expressing a c. jejuni 24-kda membrane-associated protein. antiserum raised against sonicated recombinant e. coli expressing th ...19957790451
urinary tract infection caused by quinolone-resistant campylobacter coli. 19947813508
identification of campylobacter jejuni, campylobacter coli and campylobacter lari by the nucleic acid amplification system nasbar.nasbar, an isothermal amplification technique for nucleic acids, was evaluated for the specific identification of campylobacter jejuni, camp. coli and camp. lari. a set of primers and a probe were chosen from the 16s rrna sequence of campylobacter. the probe was hybridized in solution with the amplified nucleic acids of 12 campylobacter species and nine other gram-negative bacteria. the probe was shown to hybridize specifically to the amplified single-stranded rna of camp. jejuni, camp. coli and ...19947822229
prenatal diagnosis of microbial invasion of the amniotic cavity with campylobacter coli in preterm labour. 19957833319
[an enzyme-linked immunosorbent assay for the detection of igg antibodies against urease of helicobacter pylori].we have developed an enzyme-linked immunosorbent assay (elisa) for the detection of igg antibodies against helicobacter pylori (hp) using purified hp urease as an antigen. the urease was purified from ultrasonicated extract of hp by nacl linear gradient system on deae-sepharose 4b chromatography. two molecular weight bands, 65kd and 27kd were observed on a sds-page gel in the purified urease sample. the urease antigen did not crossreact to rabbit antibodies prepared against campylobacter coli an ...19947844892
cryptolepis sanguinolenta activity against diarrhoeal bacteria.cryptolepine is the main alkaloid of cryptolepis sanguinolenta (lindl.) schlechter, a plant used in traditional medicine in west africa. the minimal inhibitory concentrations (mics) of cryptolepine, ethanol and aqueous extracts of cryptolepis sanguinolenta root were determined for 65 strains of campylobacter jejuni, 41 strains of campylobacter coli isolated from sporadic cases of gastroenteritis in portugal and 86 strains of vibrio cholerae isolated from patients with enteric infections in angol ...19947853867
campylobacter hyoilei sp. nov., associated with porcine proliferative enteritis.campylobacter hyoilei sp. nov. is the name proposed for an organism formerly described as strain rmit 32at (t = type strain) and a group of similar bacteria isolated from intestinal lesions of pigs with proliferative enteritis. the phenotypic characteristics of these organisms indicated that they are closely related to each other and are not strains of other campylobacter spp. commonly isolated from pigs. the results of probing of clai-, ecorv-, or bglii-cleaved genomic dnas from c. hyoilei stra ...19957857809
biotyping of campylobacter jejuni and campylobacter coli infections in spain.biochemical profiles were studied in 102 campylobacter isolates from patients affected by enteric disease in orense (spain) over a 1-year period. the isolates were identified as hippurate-positive campylobacter jejuni (n = 90), campylobacter coli (n = 10) and hippurate-negative c. jejuni (n = 2). seventy-seven of the hippurate-positive and both hippurate-negative c. jejuni isolates were biotyped as c. jejuni subsp. jejuni biotype 1, nine as c. jejuni subsp. jejuni biotype 2 and four as c. jejuni ...19947884224
seroreactivity to campylobacter jejuni and gangliosides in patients with guillain-barré syndrome.seroreactivities to campylobacter jejuni or campylobacter coli and to the gangliosides gm1 and gd1b were studied by quantitative and immunoglobulin class-specific elisas in 42 patients with recent onset of acute guillain-barré syndrome (gbs), in 39 patients with positive campylobacter serology but no neurologic disease, and in 52 healthy blood donors. gbs patients showed positive reactivities to c. jejuni in 57%, 26%, and 2% for igg, iga, and igm, respectively, while blood donors had correspondi ...19947930724
detection of flagellar antigen of campylobacter jejuni and campylobacter coli in canine faeces with an enzyme-linked immunosorbent assay (elisa)--new prospects for diagnosis.a new diagnostic procedure was developed to detect the flagellar antigen of campylobacter jejuni and campylobacter coli in canine faecal specimens and was tested on faecal samples from random-source dogs obtained from the local dog pound. extraction of acid-soluble proteins was performed on faecal specimens and the extracted material was evaluated using species-specific monoclonal antibodies in an enzyme-linked immunosorbent assay. the assay detected all c. jejuni or c. coli infected specimens c ...19947975201
cloning, nucleotide sequence and characterization of a gene encoding superoxide dismutase from campylobacter jejuni and campylobacter coli.genes encoding superoxide dismutase (sod: ec were cloned from campylobacter jejuni nctc 11351 and campylobacter coli ua585 by heterologous complementation of a sod-deficient escherichia coli mutant. deletion analysis of the cloned c. jejuni dna assigned the sod gene to a 1.2 kb insert and this contained an open reading frame of 660 bp. the deduced gene product of 220 amino acids was 71% identical to the e. coli iron-containing sod and 60% identical to the e. coli manganese-containing s ...19948025686
phylogenetic and molecular characterization of a 23s rrna gene positions the genus campylobacter in the epsilon subdivision of the proteobacteria and shows that the presence of transcribed spacers is common in campylobacter spp.the nucleotide sequence of a 23s rrna gene of campylobacter coli vc167 was determined. the primary sequence of the c. coli 23s rrna was deduced, and a secondary-structure model was constructed. comparison with escherichia coli 23s rrna showed a major difference in the c. coli rrna at approximately position 1170 (e. coli numbering) in the form of an extra sequence block approximately 147 bp long. pcr analysis of 31 other strains of c. coli and c. jejuni showed that 69% carried a transcribed space ...19948045890
characterization of the sat4 gene encoding a streptothricin acetyltransferase in campylobacter coli be/g4.the sat4 streptothricin resistance gene from campylobacter coli be/g4 was cloned into puc18, and its nucleotide sequence was determined. streptothricin acetyltransferase activity was detected in escherichia coli cells containing recombinant plasmid pat132 which carries the sat4 gene as an insert. the deduced amino acid sequence displayed 21-27% amino acid identity with streptothricin acetyltransferases from e. coli and streptothricin producers streptomyces lavendulae and streptomyces noursei. th ...19948056285
macro-fingerprinting analysis at the chromosomal genomic dna level of isolates of thermophilic campylobacter coli and c. jejuni, by pulsed-field gel electrophoresis.chromosomal genomic dna from 25 strains of thermophilic campylobacter coli and c. jejuni were analysed after pulsed-field gel electrophoresis (pfge) of restriction fragments generated by sali and smai to evaluate the utility of macro-fingerprinting analysis and to assess the possible subtyping of the isolates. two rare-cutting restriction enzymes, sali and smai, allowed the subclassification of eight strains of c. coli into six types. sali facilitated the subclassification of seventeen strains o ...19948070288
distribution and polymorphism of the flagellin genes from isolates of campylobacter coli and campylobacter jejuni.the complex flagellar filaments of the lio8 serogroup member campylobacter coli vc167 are composed of two highly related subunit proteins encoded by the flaa and flab genes which share 92% identity. using oligonucleotide primers based on the known dna sequence of both the flaa and flab genes from c. coli vc167 in the polymerase chain reaction, we have shown conservation of both fla genes among isolates within the lio8 heat-labile serogroup by digestion of the amplified product with psti and ecor ...19938098328
azithromycin resistance in campylobacter jejuni and campylobacter coli.the mics of erythromycin, azithromycin and ciprofloxacin were determined for 60 human fecal isolates of campylobacter. of these, 30 strains selected on the basis of their resistance to erythromycin by disk diffusion were highly resistant to both erythromycin and azithromycin. nine of these selected isolates were resistant to ciprofloxacin. the remaining 30 strains were non-selected, consecutive isolates of campylobacter susceptible to erythromycin by disk diffusion and were shown to be two- to f ...19938112360
bacteraemia caused by campylobacter spp.the genus campylobacter has become increasingly recognised as the cause of various infections. campylobacter jejuni and c coli cause acute gastroenteritis in man all over the world. c jejuni enteritis can lead to bacteraemia, but its actual incidence remains unknown. seven cases of bacteraemia caused by c jejuni or c coli are reported, from the blood of seven patients: five immune deficient adults; a newborn baby; and a patient who had had abdominal surgery. patients who develop diarrhoea as a r ...19948132835
iron-regulated proteins in outer membranes of campylobacter jejuni diarrhoea isolates and immune response to the proteins in patients.the outer membrane protein (omp) profiles from 8 campylobacter jejuni and 5 campylobacter coli fecal isolates grown under various conditions were compared by sds-page. the bacteria were grown under usual conditions, in iron-deficient medium (dip) and on iron-supplemented medium (fe). the omp profiles of most bacterial strains grown under usual conditions, or in the fe-supplemented medium, contained four major bands of approximately 31, 45, 63-66 and 97 kda, and in addition, a number of minor ban ...19948167428
use of nonradioactive dna probes to identify a campylobacter jejuni strain causing abortion.a case of human abortion due to a campylobacter infection is reported. cultures revealed two morphologically different isolates with large and small colonies respectively. using conventional methods of identification, the large colonies were identified as campylobacter jejuni and the small colonies as campylobacter coli. dot blot hybridization and determination of rdna restriction fragment patterns revealed that both colony types were the same strain of campylobacter jejuni. this observation ill ...19938223663
transformation of helicobacter pylori by chromosomal metronidazole resistance and by a plasmid with a selectable chloramphenicol resistance marker.most strains of helicobacter pylori are naturally competent for uptake of chromosomal dna. transformation frequencies for streptomycin resistance or rifampicin resistance markers ranged from 1 x 10(-4) to 1 x 10(-3) per viable cell using a plate transformation procedure. transformation of a metronidazole resistance marker (mtrr) was demonstrated when either a laboratory-derived mutant or a mtrr clinical isolate were used as the source of donor dna. mtrr was transformed at a frequency of 3 x 10(- ...19938254319
[unusual infectious complication in a pregnant woman. spontaneous abortion caused by campylobacter coli]. 19938259031
interaction of helicobacter pylori (strain 151) and campylobacter coli with human peripheral polymorphonuclear granulocytes.helicobacter pylori associated gastritis is characterized by dense mucosal inflammatory infiltrations with predominantly neutrophilic granulocytes, together with a local and systemic immune response. nevertheless, the natural course of the infection is chronic in nature, and active phagocytosis of h. pylori by mucosal granulocytes was only rarely observed. the aim of the present study was to investigate with electronmicroscopic methods the interaction of h. pylori with freshly harvested human pe ...19938280958
efficacy of filter types for detecting campylobacter jejuni and campylobacter coli in environmental water samples by polymerase chain reaction.a previously developed polymerase chain reaction (pcr) amplification of a target region in the flaa campylobacter flagellin gene was evaluated and adapted for use with environmental water samples. the ability to detect campylobacter jejuni or campylobacter coli in seeded water samples was tested with various filters after concentration and freeze-thaw lysis of the bacterial cells. a nonradioactive probe for the amplified flagellin gene fragment detected as little as 1 to 10 fg of genomic dna and ...19938285708
development of species-specific dna probes for campylobacter jejuni, campylobacter coli, and campylobacter lari by polymerase chain reaction fingerprinting.the application of polymerase chain reaction (pcr) fingerprinting assays enables discrimination between species and strains of microorganisms. pcr primers aiming at arbitrary sequences in combination with primers directed against the repetitive extragenic palindrome (rep) or enterobacterial repetitive intergenic consensus (eric) motifs generate isolate-specific dna banding patterns. analysis of these pcr fingerprints obtained for 33 isolates of campylobacter jejuni, 30 isolates of campylobacter ...19938314996
the campylobacter sigma 54 flab flagellin promoter is subject to environmental regulation.the complex flagellum of campylobacter coli vc167 is encoded by two tandemly oriented flagellin genes which are transcribed as two discrete transcriptional units from two different classes of promoters. the flab gene, which encodes the minor flab filament protein, is controlled by a sigma 54 promoter. a transcriptional fusion between a promoterless chloramphenicol acetyltransferase (cat) reporter gene cartridge and c. coli vc167 dna carrying flab transcription and translation signals, including ...19938331072
an antigenic polysaccharide from campylobacter coli serotype o:30. structure of a teichoic acid-like antigenic polysaccharide associated with the lipopolysaccharide.a water-soluble antigenic polysaccharide of high m(r) associated with the lipopolysaccharide has been isolated from phenol-water extraction of cells of campylobacter coli serotype o:30. the polysaccharide and oligosaccharide degradation products formed on o-dephosphorylation and by periodate oxidation followed by reduction have been investigated by one- and two-dimensional 1h, 13c, and 31p nmr. it is concluded that the antigenic polysaccharide has a teichoic acid-like structure with a poly-ribit ...19938349707
direct polymerase chain reaction detection of campylobacter jejuni and campylobacter coli in raw milk and dairy products.a polymerase chain reaction (pcr) method designed to sensitively detect and identify campylobacter jejuni and campylobacter coli without the need for isolating and culturing strains is described. the intergenic sequence between the flagellin genes flaa and flab was amplified and characterized with a triple primer or seminested primer approach. a total of 50 bacterial strains, 27 of c. jejuni and c. coli and 23 of other species, were tested, giving no false-positive or false-negative results. the ...19938357250
peb1, the major cell-binding factor of campylobacter jejuni, is a homolog of the binding component in gram-negative nutrient transport systems.the protein peb1 (28 kda) is a common antigen and a major cell adherence molecule of campylobacter jejuni and campylobacter coli. we created a bank of chromosomal dna fragments of c. jejuni strain 81-176 using lambda gt11. screening this bank in escherichia coli y1090 cells with antibody raised against purified peb1 enabled us to isolate and to purify a clone with a 2.6-kilobase insert expressing an immunoreactive protein of 28 kda. dna sequencing revealed that the insert contains three complete ...19938360165
[hematologic manifestations in campylobacter coli septicemia. apropos of a case].we report the case of a 71-year old male patient with a chronic lymphoid leukaemia of 10 years' duration who abruptly suffered deterioration of this general condition and haematological disturbances suggesting worsening of this blood disease (richter's syndrome). blood cultures demonstrated a campylobacter coli septicaemia. treatment with antibiotics resulted in a return to the previous clinical and biological situation. the various visceral manifestation of the infection are discussed.19938362107
characterization of a new class of tetracycline-resistance gene tet(s) in listeria monocytogenes bm4210.the nucleotide sequence of the tetracycline (tc)-minocycline (mc)-resistance determinant of plasmid pip811 from listeria monocytogenes bm4210 has been determined. the gene, designated tet(s), was identified by analysis of the start and stop codons as a coding sequence of 1923 bp, corresponding to a protein with a calculated m(r) of 72,912. the apparent 68-kda size estimated by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis of the protein characterized in a cell-free coupled transcript ...19938370538
helicobacter acinonyx sp. nov., isolated from cheetahs with gastritis.four strains of a novel helicobacter species were isolated from the stomachs of cheetahs (acinonyx jubilatus) with gastritis. these isolates were phenotypically similar to helicobacter pylori. the isolates were gram-negative, spiral bacteria which grew under microaerophilic conditions at 37 degrees c, but not at 25 or 42 degrees c, and produced urease, catalase, oxidase, alkaline phosphatase, and gamma-glutamyl transpeptidase. the isolates did not ferment glucose, mannitol, inositol, sorbitol, r ...19938379970
cloning and nucleotide sequence of the campylobacter jejuni gyra gene and characterization of quinolone resistance mutations.the gyra gene of campylobacter jejuni ua580, which encodes the a subunit of dna gyrase, was cloned and its nucleotide sequence was determined. an open reading frame of 2,589 nucleotides was identified, which could code for a polypeptide of 863 amino acids with a m(r) of 97 kda. both the nucleotide sequence and the putative amino acid sequence show ca. 50% identity with those of other gyra genes from gram-positive and gram-negative bacteria. the locations of the gyra gene on genome maps of both c ...19938384814
susceptibilities of beta-lactamase-positive and -negative strains of campylobacter coli to beta-lactam agents.the percentages of susceptibility of 28 strains of campylobacter coli to beta-lactam agents were 96% for amoxicillin and ampicillin, 57% for ticarcillin, 4% for cefoxitin and cefuroxime, 61% for cefotaxime, and 11% for ceftazidime. none of the strains were susceptible to penicillin g, piperacillin, cefazolin, cephalothin, cefamandole, and cefoperazone. all strains were susceptible to imipenem and ciprofloxacin, and 21% were susceptible to erythromycin. a beta-lactamase was detected in 68% of the ...19938390812
heterologous gene expression in campylobacter coli: the use of bacterial luciferase in a promoter probe vector.a novel promoter probe plasmid (psp73) was constructed to allow the analysis of environmentally regulated gene expression in campylobacter. the vector utilizes the luxab genes from xenorhabdus luminescens, which encode a thermostable luciferase, as reporters of gene expression. the utility of this reporter system was demonstrated by placing the expression of luxab under the transcriptional control of the flaa gene promoter.19938405932
characterization of viable but nonculturable stage of c. coli, characterized with respect to electron microscopic findings, whole cell protein and lipooligosaccharide (los) patterns.campylobacter coli ck 205, isolated from swine feces, was examined for changes in cell morphology, protein and lipooligosaccharide (los) patterns during starvation-survival experiments. bottles filled with sterile filtered a. dest. were seeded with campylobacters and incubated at 4 degrees c and 37 degrees c. transition to the nonculturable stage occurred within 48 hours (37 degrees c) and 2 weeks (4 degrees c), respectively. in contrast to the culturability the electrophoretic studies showed no ...19938451879
effect of host lineage on the virulence of campylobacter jejuni/coli in the chick embryo model.the chorioallantoic membrane (cam) inoculated chick embryo model was used to study the effect of host lineage on the virulence of campylobacter jejuni and campylobacter coli. ld50 values were used to compare the susceptibilities of chick embryos from eight inbred chicken lines to infection by four strains of c. jejuni and one strain of c. coli. differences in susceptibility were found between inbred chicken lines. these were shown not to be due to maternal antibody status, nor transfer of antibo ...19938454192
lipopolysaccharide of campylobacter coli serotype o:30. fractionation and structure of liberated core oligosaccharide.lipopolysaccharide from phenol-water extraction of cells of campylobacter coli serotype o:30 was separated as a water-insoluble gel of low m(r) from a water-soluble antigenic polysaccharide of high m(r). acetic acid hydrolysis of the ketosidic linkages to lipid a in the lipopolysaccharide furnished a core oligosaccharide. structural investigations were performed using 1h and 13c nmr, fast atom bombardment-mass spectrometry of permethylated derivatives, and methylation linkage analysis on the cor ...19938454598
evaluation of commercial antisera for serotyping heat-labile antigens of campylobacter jejuni and campylobacter coli.commercial antisera for serotyping 22 heat-labile antigens of campylobacter jejuni and campylobacter coli were evaluated by using 66 isolates from human and nonhuman sources. test results were compared with results of tests using antisera produced at the centers for disease control (cdc), atlanta, ga. all strains (three isolates of each of the 22 serotypes) were typeable with the cdc antisera. of 66 test strains, 39 (59%) were typed as the same serotype with both sets of antisera. twenty-four st ...19938463402
significance of duplicated flagellin genes in campylobacter.the complex flagellum of campylobacter coli vc167 contains two highly related (98%) flagellin subunit proteins which are produced from two 92% homologous, tandemly orientated genes, flaa and flab. mutants expressing only flaa form a full-length flagellar filament that confers slightly less than wild-type motility to the bacterium. however, flagellin mutants expressing only flab produce extremely short, truncated filaments, and are only slightly motile. we have shown that the presence of two esse ...19938464049
the role of management systems in the epidemiology of thermophilic campylobacters among poultry in eastern zone of tanzania.a total of 255 samples of droppings collected from a total of 22 different poultry units were examined for the presence of thermophilic campylobacters and the isolates biotyped using skirrow's protocol. the organisms were isolated from 90 (35.3%) of all samples. among the 22 units investigated, 13 (59%) were found to have unsatisfactory management systems, while 7 (32%) and 2 (9%) were found to have unsatisfactory and good systems respectively. significantly large numbers of isolations, 68 of 14 ...19938472771
arcobacter (campylobacter) butzleri-associated diarrheal illness in a nonhuman primate population.after dna hybridization identified an isolate from an ill rhesus macaque (macaca mulatta) as arcobacter (campylobacter) butzleri, we initiated a study to determine whether a. butzleri was associated with diarrheal disease in nonhuman primates at the yerkes primate research center. by using campy-cva medium incubated at 35 degrees c, 15 a. butzleri isolates were obtained from 14 macaques; 7 macaques were coinfected with campylobacter coli and campylobacter jejuni. a. butzleri was not isolated fro ...19938478115
quinolone resistance in clinical strains of campylobacter jejuni and campylobacter coli. 19958537280
the speciation and subtyping of campylobacter isolates from sewage plants and waste water from a connected poultry abattoir using molecular this study the distribution of phenotypes of campylobacter strains in sewage and surface waters was investigated by subtyping and by speciation of isolates from various aquatic environments. these environments included two municipal sewage plants (spa and spb) and waste water from a poultry abattoir (wwa). both the sewage plants spa and spb collected domestic and industrial waste, and spa received drain water from wwa. spb received no waste water from any meat-processing plant. the isolates w ...19958557080
enterochelin acquisition in campylobacter coli: characterization of components of a binding-protein-dependent transport system.siderophore-mediated iron uptake systems play a central role in the pathogenesis of infection for many bacterial pathogens. campylobacter species are not thought to produce siderophores, yet they are able to utilize both ferrichrome and enterochelin as sources of iron. part of an operon named ceubcde, encoding components of a periplasmic binding-protein-dependent transport (pbt) system for the uptake of a ferric siderophore from campylobacter coli, was cloned directly into escherichia coli using ...19958574410
characterization of the type strain of campylobacter coli, cip 70.80, by plasmid typing.a 1.9-kb plasmid dna fragment from the type strain of campylobacter coli, cip 70.80, was used as a probe to characterize this type strain, other c. coli type strains obtained from several culture collections, and other c. coli strains. a specific hybridization pattern was obtained, and this pattern can be used to identify, characterize, and follow up c. coli type strains in culture collections.19958590689
[the comparative identification of campylobacter strains by traditional enzymatic tests and the gene amplification reaction].39 strains of campylobacter isolated from 153 diarrhoeal children (0-3 years) were comparatively identified by the traditional enzymatic tests and by the polymerase chain reaction (pcr). the hippurate hydrolysis test appreciated 27 strains as campylobacter jejuni (69.2%) and 12 strains as campylobacter coli (30.8%). the p.c.r. realised in france has appreciated 29 strains as campylobacter jejuni (74.4%) and 10 strains as campylobacter coli (25.6%). the analysis of the results discrepancy permitt ...19958640005
ribotypes and ap-pcr fingerprints of thermophilic campylobacters from marine recreational waters.thirty-two strains of thermophilic campylobacters isolated from marine recreational water and seven reference strains were biotyped and analysed by chromosomal dna haeiii ribopatterns and ap-pcr profiles based on a random 10-mer primer (5'-caa tcg ccg t-3'). the majority of seawater isolates (90%) were campylobacter coli, and three strains were camp. jejuni. southern blot hybridization analysis showed differences between the strains, and in a numerical analysis three main clusters were formed at ...19968642012
polymerase chain reaction assay for detection of campylobacter coli and campylobacter jejuni in poultry meat.primers of 16s rrna and flaa genes were used to optimise a pcr technique for detecting thermotolerant campylobacters in poultry meat. different methods for crude dna extraction were also evaluated. the use of flaa primers and extraction of nucleic acid by boiling and proteinase k gave good results in the detection of campylobacter either in artificially or naturally contaminated foodstuffs. the lowest sensitivity limit for the pcr reaction was 10(1)-10(2) thermophilic campylobacter cells either ...19958672221
rgfgigs is an amino acid sequence required for acetyl coenzyme a binding and activity of human spermidine/spermine n1acetyltransferase.polyamine catabolism is rate limited by spermidine/spermine n1-acetyltransferase (ssat). although the amino acid sequence of ssat is known, the substrate binding and catalytic sites are not. the goal of this study was to define the region responsible for acetyl coenzyme a binding. human ssat contains a region of 20 amino acids homologous to several microbial antibiotic n-acetyltransferases. the highest homology is represented in the campylobacter coli streptothricin acetyltransferase sat4 gene, ...19968702554
ciprofloxacin resistant campylobacter spp. in humans: an epidemiological and laboratory study.from the end of april 1991 until the end of 1991, 2209 isolates of campylobacter spp. have been collected in plymouth phl of which 91 (4.1%) were resistant to ciprofloxacin. none of the 91 patients involved had taken a quinolone, but 30/91 (33%) had travelled abroad (16 to the iberian peninsula) in the three months preceding isolation of the ciprofloxacin-resistant campylobacter spp. in the case-control study 12/15 (80%) of the cases had recently consumed poultry as had 20/24 (83%) of controls w ...19968722540
restriction fragment length polymorphism of flagellin genes of campylobacter jejuni and/or c. coli isolates from egypt.the conservation of flagellin genes from thermophilic campylobacter spp. strains isolated in egypt was evaluated by a restriction fragment length polymorphism (rflp) assay. the flaa and flab genes were amplified from 59 independent clinical isolates and digested with ecori and psti, and the resulting patterns were compared with each other and with previously described rflp groups. the results indicate that the isolates fell into 14 groups for flaa and 11 groups for flab, 9 of which have been des ...19968727905
soma, a novel gene that encodes a major outer-membrane protein of synechococcus sp. pcc 7942.the outer membrane of a cyanobacterium (synechococcus sp. strain pcc 7942) contains only a few major proteins. a gene encoding one of them, soma, was cloned and characterized. based on the nucleotide sequence, soma was predicted to comprise 531 amino acids with a calculated molecular mass of 57,136 da. the deduced amino acid sequence of soma shares similarities with two bacterial cell-surface proteins, the s-layer protein of thermus thermophilus and the flagellin of campylobacter coli. the predi ...19968760925
comprehensive ribotyping scheme for heat-stable serotypes of campylobacter jejuni.strains from diverse sources belonging to all 47 heat-stable penner serotypes of campylobacter jejuni were examined for polymorphism around the 16s rrna genes. penner serotype reference strains and a group of nonserotypeable isolates were included in the study. complete typeability was obtained; 30 distinct psti and 42 haeiii polymorphisms were found. three bands were detected in almost all strains with these enzymes, confirming that three copies of the 16s rrna gene are typical for c.jejuni. by ...19968788998
analysis of hl and o serotypes of campylobacter strains by the flagellin gene typing system.we recently developed a molecular typing system for campylobacter jejuni and campylobacter coli based on restriction fragment length polymorphism analysis of the flagellin gene,flaa (i.nachamkin, k. bohachick, and c.m. patton, j. clin. microbiol. 31:1531-1536, 1993). we extended the typing system to 83 flagellin types (designated flaa-1,flaa-2, etc.) on the basis of analysis of 404 isolates of c. jejuni and c. coli including common serotypes isolated in the united states, a selection of less com ...19968789000
an environmentally regulated pilus-like appendage involved in campylobacter pathogenesis.examination of strains of campylobacter jejuni, campylobacter coli, and campylobacter fetus by electron microscopy revealed that they produced peritrichous pilus-like appendages when the bacteria were grown in the presence of bile salts. various bile-salt supplements were used and it was found that deoxycholate and chenodeoxycholic acid caused a significant enhancement of pilus production and resulted in a highly aggregative phenotype. morphologically, the pili were between 4 and 7 nm in width a ...19968793884
identification and characterization of genes required for post-translational modification of campylobacter coli vc167 flagellin.two genes have been identified in campylobacter coli vc167 which are required for the biosynthesis of post-translational modifications on flagellin proteins. the ptma gene encodes a protein of predicted m(r) 28,486 which shows significant homology to a family of alcohol dehydrogenases from a variety of bacteria. the ptmb gene encodes a protein of predicted m(r) 26,598 with significant homology to cmp-n-acetylneuraminic acid synthetase enzymes involved in sialic acid capsular biosynthesis in neis ...19968825781
antimicrobial susceptibilities of campylobacter jejuni and campylobacter coli to 12 beta-lactam agents and combinations with beta-lactamase inhibitors.the in vitro activities of 12 beta-lactam agents against 100 thermophilic campylobacter strains were tested. beta-lactamase production was detected in 88% of all strains tested. clavulanic acid was the best inhibitor by susceptibility testing. the beta-lactams which displayed high levels of in vitro activity against campylobacter isolates were imipenem, amoxicillin-clavulanic acid, and cefepime and, to a lesser degree, amoxicillin, ampicillin, and cefotaxime.19968843305
hazard identification in swine slaughter with respect to foodborne bacteria.swine slaughter is an open process with many opportunities for the contamination of the pork carcass with potentially pathogenic bacteria; however, it does not contain any point where hazards are completely eliminated. data on the prevalence of various pathogenic bacteria (aeromonas hydrophila, campylobacter coli/jejuni, listeria monocytogenes, salmonella spp., staphylococcus aureus and yersinia enterocolitica) in pigs, their growth and survival characteristics and ability to become established ...19968856371
sub-typing of animal and human campylobacter spp. using rapd.based on a 10-mer primer (5'-cctgttagcc-3'), a random amplified polymorphic dna (rapd) method for typing campylobacter coli isolated from pigs was developed. the method proved effective with a high discrimination and good reproducibility. in contrast with serotyping no untypable strains were found out of a total of 269 isolates (veterinary, food and clinical) examined. the method was also successfully applied to typing campylobacter jejuni from a similar range of sources.19968862022
the lipooligosaccharides of pathogenic gram-negative bacteria.lipooligosaccharides (loss) are the major glycolipids expressed on mucosal gram-negative bacteria, including members of the genera neisseria, haemophilus, bordetella, and branhamella. they can also be expressed on some enteric bacteria such as campylobacter jejuni and campylobacter coli strains. los is analogous to the lipopolysaccharide (lps) found in other gram-negative families. loss share similar lipid a structures with an identical array of functional activities as lpss. loss lack o-antigen ...19968894399
development of a rapid and specific colony-lift immunoassay for detection and enumeration of campylobacter jejuni, c. coli, and c. lari.contamination of retail poultry by campylobacter spp. is a significant source of human diarrheal disease. we have developed a colony-lift immunoassay (cli) for the detection of campylobacter jejuni, c. coli, and c. lari isolated from such sources and grown on selective agar medium or on filter membranes. this technique has been successfully utilized to quantify campylobacter colonies within 18 to 28 h after sampling. hydrophobic, high-protein-binding membranes were prewet with methanol and used ...19968914757
a combined polymerase chain reaction and restriction endonuclease enzyme assay for discriminating between campylobacter coli and campylobacter jejuni.a combined polymerase chain reaction and restriction endonuclease (re) enzyme assay was developed to discriminate between campylobacter coli and campylobacter jejuni. amplimers of the flaa gene obtained by pcr were digested with alui and hinfi to distinguish c. coli from c. jejuni. with alui digestion c. jejuni-specific bands were observed at 110, 140 and 160 bp and c. coli-specific bands at 293 and 147 bp. c. jejuni-specific bands of 349 and 109 bp were found by hinfi digestion but hinfi did no ...19968954352
detection of campylobacter jejuni and camp. coli in chicken faecal samples by pcr.pcr primers were selected from the flagellin gene sequences flaa and flab of campylobacter coli to amplify dna from camp. jejuni and camp. coli. when the pcr products were analysed by hybridization to an internal probe immobilized in microtitre wells, positive reactions were observed only for strains of camp. jejuni and camp. coli. the assay was used to analyse 31 chicken faecal samples. full correspondence was found between the pcr assay conducted on the enriched cultures and the standard cultu ...19968987720
specific identification of the enteropathogens campylobacter jejuni and campylobacter coli by using a pcr test based on the ceue gene encoding a putative virulence determinant.a pcr method for the rapid identification and discrimination of thermophilic campylobacter jejuni and campylobacter coli was developed by using a gene encoding a protein involved in siderophore transport (ceue). a nucleotide sequence divergence of approximately 13% in the ceue genes of c. jejuni and c. coli facilitated the design of two species-specific pcr primer sets. the specificity of the pcr amplification reactions was confirmed by using two nonradioactively labelled species-specific intern ...19979041429
integration of heterologous plasmid dna into multiple sites on the genome of campylobacter coli following natural transformation.the efficiency of homologous recombination in campylobacter coli following the introduction of dna by natural transformation was determined by using a series of nonreplicating integrative vectors containing dna fragments derived from the c. coli catalase gene. homologous recombination occurred with as little as 286 homologous bp present and was not detected when 270 bases of homology was provided. instead, when plasmids with little or no homology to the chromosome were introduced by natural tran ...19979045845
campylobacter-host cell interactions.the enteric pathogens campylobacter jejuni and campylobacter coli are a major cause of infectious diarrhoea. their ability to adhere to human epithelial cells is ubiquitous and their propensity to invade cells is also well documented and requires motility and de novo protein synthesis, as well as several host factors. the molecular basis of the interaction between campylobacters and host cells is only beginning to be elucidate. the characteristics of this interaction promise to be interesting an ...19979080607
molecular characterization of plda, the structural gene for a phospholipase a from campylobacter coli, and its contribution to cell-associated hemolysis.a gene (plda) encoding a 35.0-kda protein with significant homology to the escherichia coli outer membrane phospholipase was identified upstream of an operon encoding an enterochelin transport system in campylobacter coli. the results of this study suggest that this gene encodes an outer membrane phospholipase a in c. coli. first, expression of the plda gene product in a plda-deficient mutant of e. coli led to the restoration of phospholipase a activity. the recombinant product also partitioned ...19979119448
identification of the tetracycline resistance gene, tet(o), in streptococcus pneumoniae.five isolates of streptococcus pneumoniae resistant to tetracycline but lacking tet(m) were studied. the tetracycline resistance gene, tet(o), was detected for the first time in the pneumococcus. the gene was amplified and sequenced and found to share 99% nucleotide sequence identity and 99, 99, and 98% deduced amino acid sequence identity with the tet(o) resistance genes of streptococcus mutans, campylobacter coli, and campylobacter jejuni, respectively.19969124862
the serotype distribution of campylobacter jejuni and c. coli isolated from patients with diarrhoea and controls at tikur anbassa hospital, addis ababa, ethiopia.sixty-eight isolates of campylobacter jejuni and c. coli isolated from patients with diarrhoea (n = 630) and controls (n = 220) at tikur anbassa hospital, addis ababa, ethiopia were serotyped on the basis of the heat-labile (hl) and the heat-stable (hs) antigens, by using 16 and 34 antisera, respectively, for the two methods. with the antisera against heat labile antigens, 89.3% of the c. jejuni and 75% of the c. coli were typable. the hl serotypes 1, 2, 3, 4, 5, 6 and 7 were the most common amo ...19979129584
enterotoxigenic capacity of campylobacter coli strains isolated from humans and bovines. 19969137747
a genetic locus involved in iron utilization unique to some campylobacter strains.two genes involved in iron utilization in campylobacter coli vc167 t1 have been characterized. the cfra gene encodes a protein with a predicted mr of 77,653 which, after processing of the leader sequence, has a predicted mr of 75,635. this protein has significant sequence identity to siderophore receptors of several bacteria, and site-specific mutants defective in cfra do not synthesize one of two major iron-repressible outer membrane proteins. an adjacent gene encodes a tonb-like protein; a mut ...19979190817
comparison of disc diffusion and agar dilution methods for antibiotic susceptibility testing of campylobacter jejuni subsp. jejuni and campylobacter coli.the correlation between disc diffusion and agar dilution susceptibility testing of five antibiotics was studied against 145 campylobacter strains: 99 campylobacter jejuni subsp. jejuni and 46 campylobacter coli. the percentages of true results and 95% ci for disc diffusion for resistant strains were 100% (93.2-100%) for tetracycline (53 strains tested), 100% (77.2-100%) for ciprofloxacin (13 strains tested), 86.7% (62.1-96.3%) for nalidixic acid (15 strains tested), 100% (56.6-100%) for erythrom ...19979222038
indwelling device-related bacteremia caused by serum-susceptible campylobacter coli.two isolates of serum-susceptible campylobacter coli were recovered in a 7-day interval from blood from a patient with hepatocellular carcinoma and liver cirrhosis whose peritoneal-caval (denver's) shunt malfunctioned. identical random amplified polymorphic dna fingerprints, cellular fatty acid chromatograms, and antibiograms of the two isolates indicate that c. coli has the ability to cause catheter-related bacteremia following its colonization of the catheter.19979230411
molecular discrimination between campylobacter jejuni, campylobacter coli, campylobacter lari and campylobacter upsaliensis by polymerase chain reaction based on a novel putative gtpase gene.polymerase chain reaction (pcr) mediated dna fingerprinting has resulted in the identification of a novel campylobacter jejuni gene, encoding a gtpase protein. the gene, consisting of 383 amino acids contained semi-conserved gtp-binding sites (designated g-1 to g-4), that are characteristic for members of the gtpase protein superfamily. remarkably, this gene from c. jejuni appears to encode a member of a novel family of gtp-binding proteins, containing two separate putative gtp-binding domains, ...19979232616
differentiation of campylobacter jejuni and campylobacter coli by polymerase chain reaction.a multiplex pcr assay was developed using two primer sets for the identification and differentiation of campylobacter coli and campylobacter jejuni. primer set i amplifies a 460-bp fragment present in c. coli and c. jejuni. set ii amplifies a 160-bp target unique to c. jejuni. when the assay was performed on reference strains, amplification of c. coli yielded only the 460-bp fragment. amplification of c. jejuni generated both the 160- and 460-bp fragments. campylobacter field strains (n = 85) is ...19979232618
the flge gene of campylobacter coli is under the control of the alternative sigma factor sigma54.the flge gene encoding the flagellar hook protein of campylobacter coli vc167-t1 was cloned by immunoscreening of a genomic library constructed in lambdazap express. the flge dna sequence was 2,553 bp in length and encoded a protein with a deduced molecular mass of 90,639 da. the sequence had significant homology to the 5' and 3' sequences of the flge genes of helicobacter pylori, treponema phagedenis, and salmonella typhimurium. primer extension analysis indicated that the vc167 flge gene is co ...19979244248
invasive and enterotoxic properties in campylobacter jejuni and campylobacter coli strains isolated from humans and animals.invasive properties of 15 strains of thermotolerant campylobacter (12 c. jejuni and 3 c. coli) were studied using hela cells cultures. in four of them (3 c. jejuni and 1 c. coli), randomly selected, intestinal perfusion experiments were conducted in order to asses enterotoxigenicity. all strains were able to invade hela cells. the number of invaded hela cells ranged from 3 to 46%. in addition to their invasive properties, the 4 strains used in perfusion experiments were able to induce either a n ...19959251750
[serodiagnosis of helicobacter pylori infections by detection of immunoglobulin g antibodies with an immunoblot technique].serological testing has recently been proposed as an aid in diagnosis of h. pylori infections. in this study, an immunoblot method has been evaluated to diagnose h. pylori infection serologically by comparing 104 serum specimens from patients with a positive gram stain and/or culture result and a positive urease test on biopsy material, as well as 30 serum specimens from patients with negative urease test, and negative microscopy and culture results. thirteen bands could be identified by immunob ...19969275637
norfloxacin resistance in campylobacter jejuni and campylobacter coli isolates from swedish patients.this study focused on the frequency of campylobacter jejuni and campylobacter coli strains resistant to norfloxacin. included were 1659 consecutive stool specimens isolated between 1992 and 1995, from as many swedish patients with diarrhoea. the patients were divided with regard to place of infection and age. all strains were tested for susceptibility to norfloxacin by means of disc diffusion test on blood-agar plates. norfloxacin-resistant strains (n = 310) were furthermore tested for resistanc ...19979301992
evaluation of antibodies reactive with campylobacter jejuni in egyptian diarrhea patients.serum and stool samples were collected from 128 individuals: 96 diarrhea patients and 32 apparently healthy controls. stool specimens were cultured for enteric bacterial pathogens, while sera were screened by enzyme-linked immunosorbent assay for campylobacter jejuni-reactive antibodies. of 28 diarrhea patients who demonstrated c. jejuni-reactive antibodies (titers, > 100), 14 were culture positive for this organism. the 32 healthy controls showed significantly lower antibody titers (p < 0.05) w ...19979302201
pcr detection, identification to species level, and fingerprinting of campylobacter jejuni and campylobacter coli direct from diarrheic samples.three sets of primers were designed for pcr detection and differentiation of campylobacter jejuni and campylobacter coli. the first pcr assay was designed to coidentify c. jejuni and c. coli based on their 16s rrna gene sequences. the second pcr assay, based on the hippuricase gene sequence, identified all tested reference strains of c. jejuni and also strains of that species which lack detectable hippuricase activity. the third pcr assay, based on the sequence of a cloned (putative) aspartokina ...19979316909
antimicrobial susceptibility patterns of thermophilic campylobacter spp. from humans, pigs, cattle, and broilers in denmark.the mics of 16 antimicrobial agents were determined for 202 campylobacter jejuni isolates, 123 campylobacter coli isolates, and 6 campylobacter lari isolates from humans and food animals in denmark. the c. jejuni isolates originated from humans (75), broilers (95), cattle (29), and pigs (3); the c. coli isolates originated from humans (7), broilers (17), and pigs (99); and the c. lari isolates originated from broilers (5) and cattle (1). all isolates were susceptible to apramycin, neomycin, and ...19979333055
campylobacter hyoilei alderton et al. 1995 and campylobacter coli véron and chatelain 1973 are subjective synonyms.the taxonomic affiliation of campylobacter hyoilei was reevaluated by examining a variety of phenotypic and genotypic criteria. whole-cell protein electrophoresis and a comparison of 66 phenotypic characters revealed that reference strains of c. hyoilei were indistinguishable from campylobacter coli strains. these data were confirmed by a dna-dna hybridization level of 67% between the type strains of the two species. several species-specific assays based on pcr amplification or probe hybridizati ...19979336905
specific detection and confirmation of campylobacter jejuni by dna hybridization and pcr.conventional detection and confirmation methods for campylobacter jejuni are lengthy and tedious. a rapid hybridization protocol in which a 1,475-bp chromogen-labelled dna probe (pdt1720) and campylobacter strains filtered and grown on 0.22-micron-pore-size hydrophobic grid membrane filters (hgmfs) are used was developed. among the environmental and clinical isolates of c. jejuni, campylobacter coli, campylobacter jejuni subsp. doylei, campylobacter lari, and arcobacter nitrofigilis and a panel ...19979361442
nucleotide sequence of the staphylococcus aureus transposon, tn5405, carrying aminoglycosides resistance genes.tn5405 is a 12 kb staphylococcal composite transposon delimited by two inverted copies of the insertion sequence is1182. this transposon carries two aminoglycosides resistance genes, apha-3 and aade, an altered gene similar to sat4 from campylobacter coli be/g4, and three open reading frames of unknown functions.19979373952
[the sat4 streptothricin acetyltransferase gene of campylobacter coli: its distribution in the environment and use as epidemiological marker].the main of this study is to give evidence about the spread of streptothricin resistance within campylobacters which were isolated from animals, men and environmental sources. streptothricin resistant campylobacters were isolated over a five years period, when the use of streptothricin for ergotropic purposes was not allowed, from slurry of swine, slurry of cattle, waste water of a goose farm and waste water of a communal sewage treatment plant. the streptothricin resistance was found to be conn ...19969376053
expression of campylobacter hyoilei lipo-oligosaccharide (los) antigens in escherichia coli.campylobacter spp. are well recognized as primary pathogens in animals and in people. to isolate and define the genetic regions encoding major surface antigens of campylobacter hyoilei, genomic dna of the type strain of the species, rmit-32a, was cloned into a cosmid vector, pla2917, in escherichia coli and the resulting genomic library was screened using antiserum raised to the parent c. hyoilei strain. six cosmid clones were found to express a series of immunoreactive bands in the 15-25 kda ra ...19979387226
analysis of distribution of campylobacter jejuni and campylobacter coli in broilers by using restriction fragment length polymorphism of flagellin gene.the incidence of campylobacter jejuni and campylobacter coli in broiler farms was 33.9% (19/56). c. jejuni-positive flocks accounted for 20.0% (17/85) and c. coli-positive ones was 4.7% (4/85). there were 14 patterns (fla type) of restriction fragment length polymorphism (rflp) of flagellin a gene among these 22 strains of c. jejuni and c. coli including the standard strain c. jejuni atcc 33560. different fla types of campylobacter were isolated from broilers in different growing cycles on the s ...19979409516
differentiation of campylobacter isolates on the basis of sensitivity to boiling in water as measured by pcr-detectable dna.differential sensitivity for the release of pcr-detectable genomic dna upon boiling in water is reported for 45 campylobacter jejuni and campylobacter coli strains isolated in egypt. all of the strains released pcr-detectable dna when treated with proteinase k and sodium dodecyl sulfate. when dna was extracted from these strains by boiling in water, nine (20%) of the strains were pcr negative or resistant to boiling, suggesting the presence of boiling-sensitive and boiling-resistant phenotypes.19989435091
comparison of a novel microaerobic system with three other gas-generating systems for the recovery of campylobacter species from human faecal samples.three commercial gas-generating systems--campygen (oxoid, uk), oxoid br56 (oxoid, uk), and campypak plus (becton dickinson, usa)--and the evacuation replacement technique were compared for the recovery of campylobacter spp. from 500 human faecal samples collected from patients with gastroenteritis. four hundred fifty faecal samples were tested upon receipt in the laboratory. fifty faecal samples that had been previously found to be positive for campylobacter spp. were tested retrospectively; the ...19979447907
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