Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| most campylobacter subtypes from sporadic infections can be found in retail poultry products and food animals. | the subtypes of campylobacter isolates from human infections in two danish counties were compared to isolates from retail food samples and faecal samples from chickens, pigs and cattle. during a 1-year period, 1285 campylobacter isolates from these sources were typed by two methods: 'penner' heat-stable serotyping and automated ribotyping (riboprinting). c. jejuni was the dominating species, but c. coli was more prevalent among food and chicken isolates (16%) compared to human isolates (4%). in ... | 2006 | 16316490 |
| an investigation of the molecular mechanisms contributing to high-level erythromycin resistance in campylobacter. | the molecular mechanisms contributing to high-level erythromycin resistance in campylobacter jejuni and campylobacter coli isolates were investigated. the a2075g mutation in the 23s rrna target genes was identified in all high-level erythromycin-resistant isolates. a number of amino acid substitutions together with insertions and deletions were identified in the corresponding genes encoding l4 and l22 ribosomal proteins both of resistant and susceptible isolates. amino acid substitutions identif ... | 2006 | 16318913 |
| australian multicentre comparison of subtyping methods for the investigation of campylobacter infection. | in order to identify subtyping methods able to contribute to the surveillance or investigation of australian campylobacter infection, six genotypic and three phenotypic subtyping methods were evaluated on a collection of 84 clinical isolates collected over a 30-month period from one region in australia. the aim was to compare the logistics of various subtyping methods and examine their ability to assist in finding outbreaks or common sources of sporadic infection. the genotypic subtyping methods ... | 2006 | 16436218 |
| distribution of tetracycline resistance genes in actinobacillus pleuropneumoniae isolates from spain. | actinobacillus pleuropneumoniae is the etiological agent of porcine pleuropneumonia. tetracycline is used for therapy of this disease, and a. pleuropneumoniae carrying the tet(b) gene, coding for an efflux protein that reduces the intercellular tetracycline level, has been described previously. of the 46 tetracycline-resistant (tc(r)) spanish a. pleuropneumoniae isolates used in this study, 32 (70%) carried the tet(b) gene, and 30 of these genes were associated with plasmids. eight (17%) isolate ... | 2006 | 16436729 |
| application of pulsed-field gel electrophoresis to identify potential outbreaks of campylobacteriosis in new zealand. | since 2002, new zealand's incidence of campylobacteriosis has exceeded 300 cases per 100,000 people per annum. to evaluate genetic variation in human isolates, 183 campylobacter isolates were collected from a single clinical laboratory in christchurch: 77 during an 8-week period in spring, and the rest 3 months later over a second 8-week period in autumn. isolates were identified to the species level and subtyped using penner serotyping (campylobacter jejuni only) and pulsed-field gel electropho ... | 2006 | 16455892 |
| isolation and characterization of a cyanophage infecting the toxic cyanobacterium microcystis aeruginosa. | we isolated a cyanophage (ma-lmm01) that specifically infects a toxic strain of the bloom-forming cyanobacterium microcystis aeruginosa. transmission electron microscopy showed that the virion is composed of anisometric head and a tail complex consisting of a central tube and a contractile sheath with helical symmetry. the morphological features and the host specificity suggest that ma-lmm01 is a member of the cyanomyovirus group. using semi-one-step growth experiments, the latent period and bur ... | 2006 | 16461672 |
| natural transformation-mediated transfer of erythromycin resistance in campylobacter coli strains from turkeys and swine. | erythromycin resistance in campylobacter coli from meat animals is frequently encountered and could represent a substantial barrier to antibiotic treatment of human infections. erythromycin resistance in this organism has been associated with a point mutation (a2075g) in the 23s rrna gene. however, the mechanisms responsible for possible dissemination of erythromycin resistance in c. coli remain poorly understood. in this study, we investigated transformation-mediated acquisition of erythromycin ... | 2006 | 16461682 |
| random amplified polymorphic dna analysis of campylobacter jejuni and campylobacter coli isolated from healthy cattle and sheep. | the genetic heterogeneity among campylobacter jejuni and campylobacter coli isolates obtained from apparently healthy cattle and sheep was investigated by random amplified polymorphic dna (rapd) analysis. a total of 348 campylobacter isolates, consisting of c. jejuni (n = 218) and c. coli (n = 130), were analysed. all these isolates were successfully typed by rapd analysis. the total numbers of band patterns defined by rapd in cattle and sheep were 42 and 45, respectively. of the 42 distinct typ ... | 2006 | 16476798 |
| study on the prevalence of campylobacter spp. from chicken meat in hanoi, vietnam. | campylobacter spp. is considered to be the most common bacterial cause of human gastroenteritis worldwide. in developing countries, campylobacter spp. diarrhea is an important cause of childhood morbidity. chicken meat is known to be a major source of campylobacteriosis infection in the world. the purpose of this study was to investigate the prevalence of campylobacter spp. in chicken meat. a total of 100 samples from breast part of chicken carcass were collected from retail market in hanoi. the ... | 2006 | 17135525 |
| a pilot study on post-evisceration contamination of broiler carcasses and ready-to-sell livers and intestines (mala) with campylobacter jejuni and campylobacter coli in a high-throughput south african poultry abattoir. | to assess post-evisceration contamination of broiler carcasses, 300 samples were randomly selected during routine slaughter in the winter of 2004. the samples originated from 50 chicken carcasses, taken directly after evisceration, as well as 25 samples from ready-to-sell packages of fresh intestines (mala) and livers. the samples were taken in batches over a period of 4 weeks to allow randomised sampling from different farms of origin. conventional culture-based detection methods of campylobact ... | 2006 | 17137050 |
| relationship between the severity of hepatitis c virus-related liver disease and the presence of helicobacter species in the liver: a prospective study. | to determine the presence of helicobacter species dna in the liver of chronic hepatitis c (chc) patients with and without cirrhosis as compared to controls, and to identify the bacterial species involved. | 2006 | 17143941 |
| in vitro and in vivo complementation of the helicobacter pylori arginase mutant using an intergenic chromosomal site. | gene complementation strategies are important in validating the roles of genes in specific phenotypes. complementation systems in helicobacter pylori include shuttle vectors, which transform h. pylori at relatively low frequencies, and chromosomally based approaches. chromosomal complementation strategies are susceptible to polar effects and disruption of other h. pylori genes, leading to unwanted pleiotropic effects. | 2006 | 16961811 |
| ganglioside molecular mimicry and its pathological roles in guillain-barré syndrome and related diseases. | 2006 | 16966405 | |
| bacteriocins reduce campylobacter colonization and alter gut morphology in turkey poults. | campylobacter is a leading cause of food-borne illness in the united states. recent evidence has demonstrated that bacteriocins produced by bacillus circulans and paenibacillus polymyxa reduce cecal campylobacter colonization in broiler chickens infected with campylobacter jejuni. as campylobacter coli is the most prevalent campylobacter isolate recovered in turkeys, the objectives of the present study were to evaluate the efficacy of these bacteriocins against c. coli colonization and their inf ... | 2006 | 16977842 |
| mechanisms of transcription activation exerted by gadx and gadw at the gada and gadbc gene promoters of the glutamate-based acid resistance system in escherichia coli. | in escherichia coli the gad system protects the cell from the extreme acid stress encountered during transit through the host stomach. the structural genes gada, gadb, and gadc encode two glutamate decarboxylase isoforms and a glutamate/gamma-aminobutyrate (gaba) antiporter, respectively. glutamate decarboxylation involves both proton consumption and production of gaba, a neutral compound which is finally exported via the gadc antiporter. regulation of gada and gadbc transcription is very comple ... | 2006 | 16980449 |
| intestinal carriage of campylobacter jejuni and campylobacter coli among cattle from south-western norway and comparative genotyping of bovine and human isolates by amplified-fragment length polymorphism. | in a survey conducted in 1999-2001, the carriage of thermotolerant campylobacters in cattle was investigated, and the genetic diversity of c. jejuni within one herd was examined and compared with human isolates. c. jejuni, c. coli and other thermotolerant campylobacter spp. were isolated from intestinal contents from 26%, 3% and 2% of 804 cattle, respectively. the carriage rate was higher in calves (46%) than in adults (29%). twenty-nine c. jejuni isolates from one herd and 31 human isolates fro ... | 2006 | 16987400 |
| effect of oral administration of butyrivibrio fibrisolvens mdt-1 on experimental enterocolitis in mice. | butyrivibrio fibrisolvens mdt-1, a butyrate-producing strain, was evaluated for use as a probiotic to prevent enterocolitis. oral administration of the mdt-1 strain (10(9) cfu/dose) alleviated the symptoms of colitis (including body weight loss, diarrhea, bloody stool, organic disorder, and mucosal damage) that are induced in mice drinking water that contains 3.0% dextran sulfate sodium. in addition, myeloperoxidase (mpo) activity levels in colonic tissue were reduced, suggesting that mdt-1 miti ... | 2006 | 16988006 |
| recovery methods for detection and quantification of campylobacter depend on meat matrices and bacteriological or pcr tools. | campylobacter is one of the main causes of human foodborne bacterial disease associated with meat consumption in developed countries. therefore, the most effective approach for recovery and detection of campylobacter from meat should be determined. two hundred ninety pork skin and chine samples were inoculated with campylobacter jejuni nctc 11168 and two strains of campylobacter coli. campylobacter cells were then recovered from suspensions and enumerated by direct plating. campylobacter recover ... | 2006 | 16995511 |
| fingerprinting of campylobacter jejuni by using resolution-optimized binary gene targets derived from comparative genome hybridization studies. | the aim of this investigation was to exploit the vast comparative data generated by comparative genome hybridization (cgh) studies of campylobacter jejuni in developing a genotyping method. we examined genes in c. jejuni that exhibit binary status (present or absent between strains) within known plasticity regions, in order to identify a minimal subset of gene targets that provide high-resolution genetic fingerprints. using cgh data from three studies as input, binary gene sets were identified w ... | 2006 | 16997982 |
| detection and identification of food-borne pathogens of the genera campylobacter, arcobacter and helicobacter by multiplex pcr in poultry and poultry products. | the objective of this study was to develop a multiplex polymerase chain reaction (pcr) to detect and differentiate food-borne pathogens of the three genera campylobacter, arcobacter and helicobacter in a single step procedure. one common reverse primer and three genus-specific forward primers were designed by hybridizing to the 16s rrna of selected reference strains. besides the species with significance as food-borne pathogens isolated from poultry meat--campylobacter jejuni, campylobacter coli ... | 2006 | 17010041 |
| natural presence of campylobacter spp. in various internal organs of commercial broiler breeder hens. | campylobacter are known to cause acute bacterial gastroenteritis in humans. poultry products have been implicated as a significant source of these infections. six experiments were performed to determine whether campylobacter could be isolated naturally from the primary and secondary lymphoid organs, liver/gallbladder, and ceca of commercial broiler breeder hens. broiler breeder hens were acquired from different commercial sources during the early, middle, and late lay cycles. the birds were euth ... | 2006 | 17039849 |
| characterization of macrolide-resistant campylobacter coli isolates from food-producing animals on farms across japan during 2004. | we investigated the susceptibilities against 7 antimicrobial agents in campylobacter jejuni and c. coli isolates from food-producing animals in 2004. in comparison with the results of past surveillance, no significant difference was observed in resistance rates against all of the antimicrobials tested in campylobacter isolates. however, slight increase of erythromycin (em) resistance was found in c. coli isolates from pigs. we examined the mutation of the 23s rrna gene and their susceptibilities ... | 2006 | 17085893 |
| campylobacter jejuni multilocus sequence types in humans, northwest england, 2003-2004. | detailed understanding of the epidemiology of campylobacter is increasingly facilitated through use of universal and reproducible techniques for accurate strain differentiation and subtyping. multilocus sequence typing (mlst) enables discriminatory subtyping and grouping of isolate types into genetically related clonal complexes; it also has the advantage of ease of application and repeatability. recent studies suggest that a measure of host association may be distinguishable with this system. w ... | 2006 | 17176563 |
| prevalence and antimicrobial resistance of campylobacter jejuni and campylobacter coli isolated from raw chicken meat and human stools in korea. | prevalence of campylobacter in raw chicken meat and human stools and subsequent antibiotic resistance profiles of the pathogenic isolates obtained from 2000 through 2002 were investigated. campylobacter jejuni and campylobacter coli were isolated from 570 of the 923 raw chicken meat samples collected from traditional markets, large retail stores, or department stores in korea, resulting in the isolation rate of 61.8%. a total of 579 campylobacter isolates were obtained from raw chicken (36.3% fo ... | 2006 | 17186659 |
| genetic characterisation of the cjaab operon of campylobacter coli. | we investigated the regulation of the cjaa and cjab genes of campylobacter coli. these genes are seemingly arranged into one operon but appear to encode functionally different proteins i.e. an extracytoplasmic solute receptor and a mhs - metabolite: h+ symporter transport protein. analysis of various transcriptional cjaa and/or cjab lacz fusion constructs revealed that both genes are arranged in an operon. race analysis located the transcription start site of the cjaab operon 46 bp upstream of t ... | 2006 | 17419285 |
| the prokaryotic antecedents of the ubiquitin-signaling system and the early evolution of ubiquitin-like beta-grasp domains. | ubiquitin (ub)-mediated signaling is one of the hallmarks of all eukaryotes. prokaryotic homologs of ub (this and moad) and e1 ligases have been studied in relation to sulfur incorporation reactions in thiamine and molybdenum/tungsten cofactor biosynthesis. however, there is no evidence for entire protein modification systems with ub-like proteins and deconjugation by deubiquitinating enzymes in prokaryotes. hence, the evolutionary assembly of the eukaryotic ub-signaling apparatus remains unclea ... | 2006 | 16859499 |
| the flik protein and flagellar hook-length control. | the bacterial flagellum is a highly complex prokaryotic organelle. it is the motor that drives bacterial motility, and despite the large amount of energy required to make and operate flagella, motile organisms have a strong adaptive advantage. flagellar biogenesis is both complex and highly coordinated and it typically involves at least three two-component systems. part of the flagellum is a type iii secretion system, and it is via this structure that flagellar components are exported. the assem ... | 2007 | 17456739 |
| assessing sensitivity to antibacterial topoisomerase ii inhibitors. | both prokaryotes and eukaryotes have two major classes of topoisomerases that make transient single- or double-strand cuts in dna. while these enzymes play critical roles in cellular processes, they are also important targets of therapeutic agents. this unit describes assays to use in characterizing topoisomerase ii-targeting agents in vitro and in bacterial cells. it provides protocols for characterizing the action of small molecules against bacterial type ii topoisomerases in vitro and the in ... | 2007 | 21948169 |
| role of poultry meat in sporadic campylobacter infections in bosnia and herzegovina: laboratory-based study. | to investigate genetic diversity and specificity of campylobacter jejuni and campylobacter coli strains isolated from humans, retail poultry meat, and live farm chickens in zenica-doboj canton, bosnia and herzegovina, and identify the role of poultry meat in sporadic campylobacter infections. | 2007 | 18074419 |
| a laboratory-based survey of campylobacter infections in prahova county. | in developing countries, as well as in many western countries, members of the genus campylobacter are recognized as one of the most common cause of acute bacterial enteritis. campylobacter jejuni and campylobacter coli isolation rates have been shown to be equal to, and sometimes higher than those of other enteric pathogens. the microbiology laboratory of the local public health authority in prahova county conducted a one and a half-year laboratory-based survey of campylobacter infections in pat ... | 2007 | 18928068 |
| the absence of intervening sequences in 23s rrna genes of campylobacter coli isolates from turkeys is a unique attribute of a cluster of related strains which also lack resistance to erythromycin. | certain campylobacter strains harbor a transcribed intervening sequence (ivs) in their 23s rrna genes. following transcription, the ivs is excised, leading to fragmentation of the 23s rrna. the origin and possible functions of the ivs are unknown. furthermore, the distribution of ivs-harboring strains within campylobacter populations is poorly understood. in this study, 104 strains of campylobacter coli from turkeys, representing 27 different multilocus sequence typing-based sequence types (sts) ... | 2007 | 17189444 |
| susceptibility of bacteria isolated from pigs to tiamulin and enrofloxacin metabolites. | susceptibilities to metabolites of tiamulin (tia) and enrofloxacin (enr) were tested using selected bacteria with previously defined minimal inhibitory concentrations (mic). the tia metabolites tested were: n-deethyl-tiamulin (dtia), 2beta-hydroxy-tiamulin (2beta-htia) and 8alpha-hydroxy-tiamulin (8alpha-htia), and the enr metabolites were: ciprofloxacin (cip) and enrofloxacin n-oxide (enr-n). bacteria, all of porcine origin, were selected as representatives of bacterial infections (staphylococc ... | 2007 | 17194550 |
| antibiotic resistance of campylobacter jejuni and campylobacter coli clinical isolates from poland. | we tested 102 campylobacter jejuni and 6 campylobacter coli clinical isolates from poland. all were susceptible to erythromycin. among the tested c. jejuni isolates 55.9% and 13.7% were resistant to ciprofloxacin and tetracycline, respectively. replacement of thr86 with ile in gyra and a plasmid-borne tet(o) gene were the main resistance mechanisms for fluoroquinolones and tetracycline, respectively. | 2007 | 17210776 |
| the orphan response regulator hp1021 of helicobacter pylori regulates transcription of a gene cluster presumably involved in acetone metabolism. | helicobacter pylori is a gastric pathogen for which no nonhuman reservoir is known. in accordance with the tight adaptation to its unique habitat, the human stomach, h. pylori is endowed with a very restricted repertoire of regulatory proteins. nevertheless, the three complete two-component systems of h. pylori were shown to be involved in the regulation of important virulence traits like motility and acid resistance and in the control of metal homeostasis. hp1021 is an orphan response regulator ... | 2007 | 17220217 |
| evaluation of genetic markers and molecular typing methods for prediction of sources of campylobacter jejuni and c. coli infections. | campylobacter jejuni and c. coli isolates from poultry, cattle, and humans were studied using pulsed-field gel electrophoresis (pfge) and pcr of candidate livestock-associated marker genes. human isolates showed 5.7 and 61% overlap with cattle and poultry isolates, respectively, by use of pfge. no unambiguous association was found between marker genes (the cj1321 and cj1324 genes) and livestock-associated isolates. | 2007 | 17220252 |
| antibiotic resistance and resistance mechanisms in campylobacter jejuni and campylobacter coli. | campylobacter jejuni and campylobacter coli are recognized as the most common causative agents of bacterial gastroenteritis in the world and infections with these organisms occur more frequently than do infections due to salmonella species, shigella species, or escherichia coli 0157:h7. the incidence of human campylobacter infections has increased markedly in both developed and developing countries worldwide and, more significantly, so has the rapid emergence of antibiotic-resistant campylobacte ... | 2007 | 18031331 |
| gastrointestinal disease in simian immunodeficiency virus-infected rhesus macaques is characterized by proinflammatory dysregulation of the interleukin-6-janus kinase/signal transducer and activator of transcription3 pathway. | gastrointestinal disease and inflammation are common sequelae of human and simian immunodeficiency virus (siv) infection. nevertheless, the molecular mechanisms that lead to gastrointestinal dysfunction remain unclear. we investigated regulation of the interleukin (il)-6-jak-stat3 pathway in jejunum and colon, collected at necropsy, from 10 siv-infected macaques with diarrhea (group 1), 10 non-siv-infected macaques with diarrhea (group 2), and 7 control uninfected macaques (group 3). all group 1 ... | 2007 | 18055558 |
| multiplex pcr for direct identification of campylobacter spp. in human and chicken stools. | differentiation between campylobacter jejuni and campylobacter coli is problematic in clinical specimens due to fastidious growth requirements and limited biochemical tests. this study describes a rapid, multiplex pcr protocol for the direct detection and differentiation of c. jejuni and c. coli in stools. an evaluation was carried out of this multiplex protocol based on the detection of cadf (genus specific), and hipo (c. jejuni) and asp (c. coli) genes, using stool from patients with campyloba ... | 2007 | 17893173 |
| composite sequence proteomic analysis of protein biomarkers of campylobacter coli, c. lari and c. concisus for bacterial identification. | protein biomarkers observed in the matrix-assisted laser desorption/ionization time-of-flight mass spectra (maldi-tof-ms) of cell lysates of three strains of campylobacter coli, two strains of c. lari and one strain of c. concisus have been identified by 'bottom-up' proteomic techniques. the significant findings are as follows. first, the protein biomarkers identified were: phna-related protein, 4-oxalocrotonate tautomerase (dmpi)-related protein, nifu-like protein, cytochrome c, dna-binding pro ... | 2007 | 17893805 |
| prevalence and strain diversity of thermophilic campylobacters in cattle, sheep and swine farms. | to determine prevalence and strain diversity of thermophilic campylobacters in healthy ruminants and swine. | 2007 | 17897201 |
| quantification and differentiation of campylobacter jejuni and campylobacter coli in raw chicken meats using a real-time pcr method. | campylobacter species are one of the most common causes of bacterial diarrhea in humans worldwide. the consumption of foods contaminated with two campylobacter species, c. jejuni and c. coli, is usually associated with most of the infections in humans. in this study, a rapid, reliable, and sensitive multiplex real-time quantitative pcr was developed for the simultaneous detection, identification, and quantification of c. jejuni and c. coli. in addition, the developed method was applied to the 50 ... | 2007 | 17900077 |
| predictive model for inactivation of campylobacter spp. by heat and high hydrostatic pressure. | campylobacter represents one of the leading causes of foodborne enteritis. poultry and its products frequently transmit the pathogen. the objective of the present study was to model predictively the short-term inactivation of campylobacter in a ready-to-eat poultry product to develop an economic high-pressure treatment. we inactivated baroresistant strains of campylobacter jejuni and campylobacter coli, grown to stationary phase on nutrient agar and inoculated in poultry meat slurry, by heat and ... | 2007 | 17900078 |
| spontaneous mutation frequency and emergence of ciprofloxacin resistance in campylobacter jejuni and campylobacter coli. | we analysed the contribution of spontaneous mutation frequency to the evolution of ciprofloxacin resistance and the diversity of mutations in the quinolone resistance-determining region (qrdr) of gyra and in the intergenic region, cmer-cmea, of the cmeabc efflux pump in campylobacter jejuni and campylobacter coli. | 2007 | 17911389 |
| campylobacter flagella: not just for motility. | campylobacter jejuni and campylobacter coli are among the major causes of diarrheal disease worldwide. the motility imparted by the polar flagella of these pathogens is required for colonization of the mucus lining of the gastrointestinal tract. however, recent studies have revealed a more complex role for flagella in campylobacter pathogenesis that includes the ability to secrete non-flagellar proteins that modulate virulence and the co-regulation of secreted and non-secreted virulence factors ... | 2007 | 17920274 |
| expanding the helicobacter pylori genetic toolbox: modification of an endogenous plasmid for use as a transcriptional reporter and complementation vector. | helicobacter pylori is an important human pathogen. however, the study of this organism is often limited by a relative shortage of genetic tools. in an effort to expand the methods available for genetic study, an endogenous h. pylori plasmid was modified for use as a transcriptional reporter and as a complementation vector. this was accomplished by addition of an escherichia coli origin of replication, a kanamycin resistance cassette, a promoterless gfpmut3 gene, and a functional multiple clonin ... | 2007 | 17921278 |
| epidemiology, relative invasive ability, molecular characterization, and competitive performance of campylobacter jejuni strains in the chicken gut. | one hundred forty-one campylobacter jejuni isolates from humans with diarrhea and 100 isolates from retailed poultry meat were differentiated by flaa typing. the bacteria were isolated in a specific geographical area (dunedin) in new zealand over a common time period. twenty nine flaa types were detected, one of which (flaa restriction fragment length polymorphism type 15 [flaa-15]) predominated among isolates from humans ( approximately 30% of isolates). this strain was of low prevalence (5% of ... | 2007 | 17921281 |
| diversity of campylobacter coli genotypes in the lower porcine gastrointestinal tract at time of slaughter. | to compare the genotypes of campylobacter coli obtained from the rectal and ileal samples of pigs at the time of slaughter. | 2007 | 17922816 |
| characterization of campylobacter phages including analysis of host range by selected campylobacter penner serotypes. | the predominant food borne pathogen in the western world today is campylobacter. campylobacter specific bacteriophages (phages) have been proposed as an alternative agent for reducing the burden of campylobacter in broilers. one concern in relation to phage biocontrol is the narrow host range often displayed by phages. to identify the potential of phages as a campylobacter reducing agent we needed to determine their infectivity on a panel of isolates representing the campylobacter strains found ... | 2007 | 17945022 |
| development of a multiplex pcr assay for identification of campylobacter coli, campylobacter fetus, campylobacter hyointestinalis subsp. hyointestinalis, campylobacter jejuni, campylobacter lari and campylobacter upsaliensis. | a multiplex pcr assay has been developed for the identification of the six common campylobacter taxa associated with human gastroenteritis and/or septicaemia, namely campylobacter coli, campylobacter fetus, campylobacter hyointestinalis subsp. hyointestinalis, campylobacter jejuni, campylobacter lari and campylobacter upsaliensis. the assay was developed using a combination of newly designed and published primers. it provided a specific pcr product for each of the five campylobacter species and ... | 2007 | 17965346 |
| effects of short-chain nitrocompounds against campylobacter jejuni and campylobacter coli in vitro. | effects of 2-nitro-1-propanol, 2-nitroethanol, nitroethane, and 2-nitro-methyl-propionate (0, 10, and 20 mm) on growth of campylobacter jejuni were tested during culture in bolton broth adjusted to ph 5.6, 7.0, or 8.2. the nitrocompounds were similarly tested against c. coli but at ph 8.2 only. viable cell counts measured during incubation revealed main effects (p < 0.05) of all nitrocompounds on the survivability of c. jejuni. an effect of ph (p < 0.05) on the survivability of c. jejuni during ... | 2007 | 17995842 |
| pulsed-plasma gas-discharge inactivation of microbial pathogens in chilled poultry wash water. | a pulsed-plasma gas-discharge (ppgd) system was developed for the novel decontamination of chilled poultry wash water. treatment of poultry wash water in the plasma generation chamber for up to 24 s at 4 degrees c reduced escherichia coli nctc 9001, campylobacter jejuni atcc 33560, campylobacter coli atcc 33559, listeria monocytogenes nctc 9863, salmonella enterica serovar enteritidis atcc 4931, and s. enterica serovar typhimurium atcc 14028 populations to non-detectable levels (< or = 8 log cfu ... | 2007 | 18095434 |
| the complete genome sequence and analysis of the epsilonproteobacterium arcobacter butzleri. | arcobacter butzleri is a member of the epsilon subdivision of the proteobacteria and a close taxonomic relative of established pathogens, such as campylobacter jejuni and helicobacter pylori. here we present the complete genome sequence of the human clinical isolate, a. butzleri strain rm4018. | 2007 | 18159241 |
| [enzyme-linked immunosorbent assay in the diagnosis of campylobacteriosis]. | campylobacter jejuni and campylobacter coli are the bacterial cause of human gastroenteritis commonly reported worldwide. the serodiagnosis of campylobacter infections is not routinely done in poland so the aim of this study was to evaluation of elisa in the diagnosis ofcampylobacteriosis. serum samples obtained from 145 patients with gastroenteritis were tested by elisa with 7 different heat-stable antigens of c. jejuni and one of c. coli and by the commercial virion/serion elisa with purified ... | 2007 | 18416128 |
| tigecycline in the treatment of complicated intra-abdominal and complicated skin and skin structure infections. | tigecycline, a glycylcycline related to the tetracycline class of antibiotics, represents a new option for the treatment of complicated intra-abdominal and complicated skin and skin structure infections. it displays favorable activity in vitro against the most common causative gram-positive, gram-negative and anaerobic pathogens. in addition, tigecycline demonstrates activity against drug-resistant pathogens such as methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococci, a ... | 2007 | 18516315 |
| clonal complexes of campylobacter jejuni identified by multilocus sequence typing are reliably predicted by restriction fragment length polymorphism analyses of the flaa gene. | multilocus sequence typing (mlst) has provided important new insights into the population structure of campylobacter jejuni and is rapidly becoming the gold standard for typing this species. however, the methodology is comparatively costly and slow to perform for the routine surveillance testing of large numbers of isolates required by public health laboratories. restriction fragment length polymorphism analysis of the flaa gene (rflp-flaa) and sequencing of the variable region in the fla locus ... | 2007 | 17093018 |
| clonal complexes of campylobacter jejuni identified by multilocus sequence typing are reliably predicted by restriction fragment length polymorphism analyses of the flaa gene. | multilocus sequence typing (mlst) has provided important new insights into the population structure of campylobacter jejuni and is rapidly becoming the gold standard for typing this species. however, the methodology is comparatively costly and slow to perform for the routine surveillance testing of large numbers of isolates required by public health laboratories. restriction fragment length polymorphism analysis of the flaa gene (rflp-flaa) and sequencing of the variable region in the fla locus ... | 2007 | 17093018 |
| identification of helicobacter pylori genes that contribute to stomach colonization. | chronic infection of the human stomach by helicobacter pylori leads to a variety of pathological sequelae, including peptic ulcer and gastric cancer, resulting in significant human morbidity and mortality. several genes have been implicated in disease related to h. pylori infection, including the vacuolating cytotoxin and the cag pathogenicity island. other factors important for the establishment and maintenance of infection include urease enzyme production, motility, iron uptake, and stress res ... | 2007 | 17101654 |
| identification of helicobacter pylori genes that contribute to stomach colonization. | chronic infection of the human stomach by helicobacter pylori leads to a variety of pathological sequelae, including peptic ulcer and gastric cancer, resulting in significant human morbidity and mortality. several genes have been implicated in disease related to h. pylori infection, including the vacuolating cytotoxin and the cag pathogenicity island. other factors important for the establishment and maintenance of infection include urease enzyme production, motility, iron uptake, and stress res ... | 2007 | 17101654 |
| specific detection of campylobacter jejuni from faeces using single nucleotide polymorphisms. | specimens of human faeces were tested by a rapid strategy for detection of campylobacter jejuni lineages by the presence of specific single nucleotide polymorphisms (snps) based on the c. jejuni multi locus sequence typing (mlst) scheme. this strategy was derived from analysis of the mlst databases to identify clonal complex specific snps followed by the design of real-time pcr assays to enable identification of six major c. jejuni clonal complexes associated with cases of human infection. the o ... | 2007 | 17109769 |
| specific detection of campylobacter jejuni from faeces using single nucleotide polymorphisms. | specimens of human faeces were tested by a rapid strategy for detection of campylobacter jejuni lineages by the presence of specific single nucleotide polymorphisms (snps) based on the c. jejuni multi locus sequence typing (mlst) scheme. this strategy was derived from analysis of the mlst databases to identify clonal complex specific snps followed by the design of real-time pcr assays to enable identification of six major c. jejuni clonal complexes associated with cases of human infection. the o ... | 2007 | 17109769 |
| comparison of antimicrobial susceptibility testing of campylobacter spp. by the agar dilution and the agar disk diffusion methods. | the correlation and the level of agreement between the standardized agar dilution and the agar disk diffusion methods for antimicrobial susceptibility testing of campylobacter were investigated. a high-level agreement between the two methods was evident for aminoglycosides and fluoroquinolones, while a low-level agreement was observed for other antibiotics. | 2007 | 17122005 |
| comparison of antimicrobial susceptibility testing of campylobacter spp. by the agar dilution and the agar disk diffusion methods. | the correlation and the level of agreement between the standardized agar dilution and the agar disk diffusion methods for antimicrobial susceptibility testing of campylobacter were investigated. a high-level agreement between the two methods was evident for aminoglycosides and fluoroquinolones, while a low-level agreement was observed for other antibiotics. | 2007 | 17122005 |
| a multi-centre prospective case-control study of campylobacter infection in persons aged 5 years and older in australia. | there are an estimated 277,000 cases of campylobacteriosis in australia each year, most of which are thought to be sporadically acquired. to explore causes for these infections, we conducted a multi-centre case-control study of patients and community controls across five australian states during 2001-2002. a total of 881 campylobacter cases and 833 controls aged 5 years were recruited into the study. crude logistic analyses were conducted within various food and non-food exposure groups. a final ... | 2007 | 17134530 |
| a multi-centre prospective case-control study of campylobacter infection in persons aged 5 years and older in australia. | there are an estimated 277,000 cases of campylobacteriosis in australia each year, most of which are thought to be sporadically acquired. to explore causes for these infections, we conducted a multi-centre case-control study of patients and community controls across five australian states during 2001-2002. a total of 881 campylobacter cases and 833 controls aged 5 years were recruited into the study. crude logistic analyses were conducted within various food and non-food exposure groups. a final ... | 2007 | 17134530 |
| conjugative transfer of chromosomally encoded antibiotic resistance from helicobacter pylori to campylobacter jejuni. | many strains of helicobacter pylori are naturally competent for transformation and able to transfer chromosomal dna among different isolates using a conjugation-like mechanism. in this study, we sought to determine whether h. pylori can transfer dna into campylobacter jejuni, a closely related species of the campylobacterales group. to monitor the transfer, a chromosomally encoded streptomycin resistance cassette prearranged by a specific mutation in the rpsl gene of h. pylori was used. mating o ... | 2007 | 17135441 |
| conjugative transfer of chromosomally encoded antibiotic resistance from helicobacter pylori to campylobacter jejuni. | many strains of helicobacter pylori are naturally competent for transformation and able to transfer chromosomal dna among different isolates using a conjugation-like mechanism. in this study, we sought to determine whether h. pylori can transfer dna into campylobacter jejuni, a closely related species of the campylobacterales group. to monitor the transfer, a chromosomally encoded streptomycin resistance cassette prearranged by a specific mutation in the rpsl gene of h. pylori was used. mating o ... | 2007 | 17135441 |
| cryptic plasmids isolated from campylobacter strains represent multiple, novel incompatibility groups. | three small, cryptic plasmids from the multi-drug-resistant (mdr) campylobacter coli strain rm2228 and one small, cryptic plasmid from the mdr campylobacter jejuni strain rm1170 were sequenced and characterized. pcc2228-1 has some similarity to firmicutes repl family plasmids that replicate via a rolling-circle mechanism. pcc2228-2 is a theta-replicating, iteron-containing plasmid (icp) that is a member of the same incompatibility (inc) group as previously described campylobacter shuttle vectors ... | 2007 | 17064774 |
| longitudinal study of finnish campylobacter jejuni and c. coli isolates from humans, using multilocus sequence typing, including comparison with epidemiological data and isolates from poultry and cattle. | we describe a study on the application of multilocus sequence typing for the analysis of campylobacter jejuni and c. coli isolates from human domestically acquired infections in the helsinki-uusimaa area of finland in 1996, 2002, and 2003. in addition, isolates from poultry meat and fecal samples of cattle from the seasonal peak (july to september) in 2003 were included in the study. in total, 361 finnish c. jejuni and c. coli strains were typed. sequence type 45 (st-45) (45%), st-21 (21%), and ... | 2007 | 17085689 |
| genome sequence of a clinical isolate of campylobacter jejuni from thailand. | campylobacter jejuni cg8486, which belongs to the hs4 complex, was isolated from a patient with inflammatory diarrhea in thailand. this strain caused a diarrheal disease in ferrets comparable to that caused by c. jejuni strain 81-176, but it was much less invasive for epithelial cells in vitro than 81-176. complete genome sequencing of cg8486 revealed a 1.65-mb genome that was very similar to the other two published genomes of clinical isolates of c. jejuni, the genomes of 81-176 and nctc 11168, ... | 2007 | 17438034 |
| detection by pcr of eight groups of enteric pathogens in 4,627 faecal samples: re-examination of the english case-control infectious intestinal disease study (1993-1996). | the english case-control infectious intestinal disease study (1993-1996) failed to detect an enteric pathogen or toxin in 49% of cases of gastroenteritis. in the present study, polymerase chain reaction (pcr) assays were applied to dna and cdna generated from 4,627 faecal samples from cases and controls archived during the original study for the detection of norovirus, rotavirus, sapovirus, campylobacter spp., salmonella spp., enteroaggregative escherichia coli, cryptosporidium spp., and giardia ... | 2007 | 17447091 |
| use of suppression subtractive hybridisation to extend our knowledge of genome diversity in campylobacter jejuni. | previous studies have sought to identify a link between the distribution of variable genes amongst isolates of campylobacter jejuni and particular host preferences. the genomic sequence data available currently was obtained using only isolates from human or chicken hosts. in order to identify variable genes present in isolates from alternative host species, five subtractions between c. jejuni isolates from different sources (rabbit, cattle, wild bird) were carried out, designed to assess genomic ... | 2007 | 17470265 |
| campylobacter jejuni colonization and transmission in broiler chickens: a modelling perspective. | campylobacter jejuni is one of the most common causes of acute enteritis in the developed world. the consumption of contaminated poultry, where c. jejuni is believed to be a commensal organism, is a major risk factor. however, the dynamics of this colonization process in commercially reared chickens is still poorly understood. quantification of these dynamics of infection at an individual level is vital to understand transmission within populations and formulate new control strategies. there are ... | 2007 | 17472905 |
| relapsing campylobacter coli bacteremia with reactive arthritis in a patient with x-linked agammaglobulinemia. | a patient genetically diagnosed with x-linked agammaglobulinemia repeatedly developed bacteremia due to campylobacter coli (c. coli) for one year and seven months in spite of immunoglobulin replacement therapy. throughout the clinical course, c. coli with identical genetic patterns was repeatedly isolated from both blood and stool cultures, thus indicating that the patient had latent intestinal infection. the bacteremia was always accompanied by reactive arthritis. since the immunoglobulin level ... | 2007 | 17473499 |
| campylobacter antimicrobial drug resistance among humans, broiler chickens, and pigs, france. | we describe isolates from human campylobacter infection in the french population and the isolates' antimicrobial drug resistance patterns since 1986 and compare the trends with those of isolates from broiler chickens and pigs from 1999 through 2004. among 5,685 human campylobacter isolates, 76.2% were c. jejuni, 17.2% c. coli, and 5.0% c. fetus. resistance to nalidixic acid increased from 8.2% in 1990 to 26.3% in 2004 (p < 10(-3)), and resistance to ampicillin was high over time. nalidixic acid ... | 2007 | 17479889 |
| host-associated genetic import in campylobacter jejuni. | host association of campylobacter jejuni was analyzed by using multilocus sequence typing data for 713 isolates from chickens and bovids (cattle and sheep). commonly used summary measures of genotypes (sequence type and clonal complex) showed poor accuracy, but a method using the full allelic profile showed 80% accuracy in distinguishing isolates from these 2 host groups. we explored the biologic basis of more accurate results with allelic profiles. strains isolated from specific hosts have impo ... | 2007 | 17479890 |
| survey of campylobacter, salmonella and mycoplasmas in house crows (corvus splendens) in malaysia. | house crows (corvus splendens) in selangor, malaysia were examined for the presence of campylobacter species, salmonella species, mycoplasma gallisepticum and mycoplasma synoviae by serology, culture and pcr. for the detection of campylobacter and salmonella species swabs were taken either from the intestine or cloaca. for the detection of mycoplasmas, swabs were taken either from the choanal cleft or trachea for culture and pcr and serum samples were tested by the rapid serum agglutination (rsa ... | 2007 | 17483380 |
| campylobacter in finnish organic laying hens in autumn 2003 and spring 2004. | a total of 642 fecal samples and 360 table eggs from finnish organic laying hens were collected in autumn 2003 (19 farms) and spring 2004 (17 farms) and studied for the presence of campylobacter. in autumn, 84% of the farms were positive for campylobacter and in spring, 76%. the percentage of positive samples within a flock varied between 5 and 100%. in addition, campylobacter was isolated in a single eggshell sample. campylobacter jejuni was the species isolated most often, although campylobact ... | 2007 | 17495096 |
| development and application of real-time pcr assays for quantification of erm genes conferring resistance to macrolides-lincosamides-streptogramin b in livestock manure and manure management systems. | erythromycin and tylosin are commonly used in animal production, and such use is perceived to contribute to the overall antimicrobial resistance (ar) reservoirs. quantitative measurements of this type of ar reservoir in microbial communities are required to understand ar ecology (e.g., emergence, persistence, and dissemination). we report here the development, validation, and use of six real-time pcr assays for quantifying six classes of erm genes (classes a through c, f, t, and x) that encode t ... | 2007 | 17496134 |
| phylogenetic relationships of campylobacter jejuni based on pora sequences. | campylobacter porins are the dominant major outer membrane protein (momp) of these bacteria. they are composed of hypervariable, surface-exposed, peptide loops and membrane-embedded, conserved peptide regions. porins are functionally important and may also be useful for molecular subtyping methods but have not yet been well characterized. we therefore sequenced the pora gene from 39 campylobacter isolates, including multilocus sequence type (mlst) reference strains, isolates from patients with t ... | 2007 | 17496947 |
| role of the plasmid-encoded tet(o) gene in tetracycline-resistant clinical isolates of campylobacter jejuni and campylobacter coli. | the prevalence of tetracycline resistance, tetracycline mics and tet(o) gene localization were investigated in 83 campylobacter isolates from patients suffering from acute gastroenteritis in germany. combined biochemical and molecular markers identified 74 isolates (89 %) as campylobacter jejuni, including seven atypical isolates that failed to hydrolyse hippurate, and nine isolates (11 %) as campylobacter coli. tetracycline resistance was detected in six out of nine campylobacter coli isolates ... | 2007 | 17510271 |
| development of experimental genetic tools for campylobacter fetus. | molecular analysis of the virulence mechanisms of the emerging pathogen campylobacter fetus has been hampered by the lack of genetic tools. we report the development and functional analysis of escherichia coli-campylobacter shuttle vectors that are appropriate for c. fetus. some vectors were constructed based on the known campylobacter coli plasmid pip1455 replicon, which confers a wide host range in campylobacter spp. versatility in directing gene expression was achieved by introducing a strong ... | 2007 | 17513579 |
| heterogeneity of a campylobacter jejuni protein that is secreted through the flagellar filament. | cj0859c, or fspa, is a small, acidic protein of campylobacter jejuni that is expressed by a sigma(28) promoter. analysis of the fspa gene in 41 isolates of c. jejuni revealed two overall variants of the predicted protein, fspa1 and fspa2. secretion of fspa occurs in broth-grown bacteria and requires a minimum flagellar structure. the addition of recombinant fspa2, but not fspa1, to int407 cells in vitro resulted in a rapid induction of apoptosis. these data define a novel c. jejuni virulence fac ... | 2007 | 17517862 |
| common genomic features of campylobacter jejuni subsp. doylei strains distinguish them from c. jejuni subsp. jejuni. | campylobacter jejuni has been divided into two subspecies: c. jejuni subsp. jejuni (cjj) and c. jejuni subsp. doylei (cjd). nearly all of the c. jejuni strains isolated are cjj; nevertheless, although cjd strains are isolated infrequently, they differ from cjj in two key aspects: they are obtained primarily from human clinical samples and are associated often with bacteremia, in addition to gastroenteritis. in this study, we utilized multilocus sequence typing (mlst) and a dna microarray-based c ... | 2007 | 17535437 |
| prevalence of campylobacter spp isolated from grower-finisher pigs in ontario. | this study aimed to establish the prevalence of campylobacter spp. in 80 ontario grower-finisher pig herds. ninety-nine percent of the isolates yielded campylobacter, c coli being the most common species detected. control of this microorganism must rely on careful food processing and storage of pork, rather than on an on-farm approach. | 2007 | 17542372 |
| expression patterns and role of the cadf protein in campylobacter jejuni and campylobacter coli. | binding of campylobacter jejuni and campylobacter coli to host fibronectin is mediated by the 37 kda outer membrane protein cadf. immunoblot analysis of 58 c. jejuni and c. coli isolates of human and animal origin showed that cadf is expressed in every strain. in most c. jejuni isolates, a 37 kda band (p37) and a less-prominent 32 kda band (p32) reacted with the antibodies. in c. coli isolates, cadf was consistently larger with sizes of 39 kda (p39) and 34 kda (p34), respectively. pcr analysis a ... | 2007 | 17573935 |
| evaluation of different plate media for direct cultivation of campylobacter species from live broilers. | accurate identification and optimal culturing procedures for campylobacter spp. from live broilers are needed for epidemiological studies. because there is no standardized protocol, we designed and conducted studies to evaluate different selective media for the culturing and isolation of campylobacter spp. from cecal and fecal samples obtained from battery-reared and commercial broilers. five media selective for campylobacter were evaluated: campylobacter agar base, campylobacter, campy-line, mo ... | 2007 | 17575175 |
| prevalence and antimicrobial resistance of campylobacter in us dairy cattle. | to obtain an overview of the prevalence and antimicrobial resistance of campylobacter in faeces of us dairy cows in 2002. | 2007 | 17578422 |
| distribution and ecology of campylobacters in coastal plain streams (georgia, united states of america). | campylobacter is the leading cause of bacterium-associated diarrhea in the united states and most developed countries. while this disease is considered a food-borne disease, many clinical cases cannot be linked to a food source. in rural and agrarian areas environmental transmission may be an important factor contributing to case loads. here we investigated the waterborne prevalence of campylobacters in a mixed-use rural watershed in the coastal plain of southern georgia (united states). six sit ... | 2007 | 17172457 |
| distribution and ecology of campylobacters in coastal plain streams (georgia, united states of america). | campylobacter is the leading cause of bacterium-associated diarrhea in the united states and most developed countries. while this disease is considered a food-borne disease, many clinical cases cannot be linked to a food source. in rural and agrarian areas environmental transmission may be an important factor contributing to case loads. here we investigated the waterborne prevalence of campylobacters in a mixed-use rural watershed in the coastal plain of southern georgia (united states). six sit ... | 2007 | 17172457 |
| new consensus guidelines from the clinical and laboratory standards institute for antimicrobial susceptibility testing of infrequently isolated or fastidious bacteria. | the clinical and laboratory standards institute (clsi) recently published a new laboratory guideline for antimicrobial susceptibility testing of infrequently encountered or fastidious bacteria not covered in previous clsi publications. the organisms include aeromonas species, bacillus species, and vibrio species that may cause infections following environmental exposure. fastidious organisms that may cause endocarditis or medical device infections include abiotrophia and granulicatella species; ... | 2007 | 17173232 |
| multilocus sequence typing performed on campylobacter coli isolates from humans, broilers, pigs and cattle originating in denmark. | to assess whether campylobacter coli isolated from different sources in denmark constitute separate populations. | 2007 | 17584467 |
| use of multilocus sequence typing to investigate the association between the presence of campylobacter spp. in broiler drinking water and campylobacter colonization in broilers. | the presence of campylobacters in broiler chickens and throughout the broiler water delivery systems of 12 farms in northeastern scotland was investigated by sensitive enrichment methods and large-volume filtration. campylobacter presence was independent of the water source and whether the water was treated. the genotypes of campylobacter jejuni isolates recovered from chickens and various locations within the water delivery systems were compared by multilocus sequence typing. matching strains i ... | 2007 | 17586665 |
| [antimicrobial susceptibilities of campylobacter sp strains isolated from humans in 2005 to 2006 in bielsko-biala region, poland]. | campylobacter jejuni and campylobacter coli are frequent causes of bacterial gastroeneritis in humans worldwide. campylobacteriois is usually a self-limiting disease and therapy with antibiotics is required in severe clinical infections. the objective [corrected] of this study was to determine the antibiotic resistance of c. jejuni and c. coli isolated from humans with diarrhea during 2005-2006 in bielsko-biala region in poland. the mics of ciprofloxacin, tetracycline, erythromycin, gentamicin a ... | 2007 | 17601030 |
| campylobacter succession in broiler chickens. | the competitive ability of campylobacter coli or12 over c. jejuni or1 has been examined in experimental broiler chickens following the observation that c. coli replaced an established c. jejuni intestinal colonisation within commercial chicken flocks reared outdoors [el-shibiny, a., connerton, p.l., connerton, i.f., 2005. enumeration and diversity of campylobacters and bacteriophages isolated during the rearing cycles of free-range and organic chickens. appl. environ. microbiol. 71, 1259-1266]. ... | 2007 | 17628357 |
| the persistence of fluoroquinolone-resistant campylobacter in poultry production. | the use of antibiotics in food animal production has been associated with antibiotic-resistant infections in humans. in 2005, the food and drug administration (fda) banned fluoroquinolone use in u.s. poultry production in order to reduce the prevalence of fluoroquinolone-resistant campylobacter. little is known about the potential efficacy of this policy. | 2007 | 17637919 |
| flagellin glycans from two pathovars of pseudomonas syringae contain rhamnose in d and l configurations in different ratios and modified 4-amino-4,6-dideoxyglucose. | flagellins from pseudomonas syringae pv. glycinea race 4 and pseudomonas syringae pv. tabaci 6605 have been found to be glycosylated. glycosylation of flagellin is essential for bacterial virulence and is also involved in the determination of host specificity. flagellin glycans from both pathovars were characterized, and common sites of glycosylation were identified on six serine residues (positions 143, 164, 176, 183, 193, and 201). the structure of the glycan at serine 201 (s201) of flagellin ... | 2007 | 17644592 |
| sequence variation in the outer membrane protein-encoding gene cmec, conferring multidrug resistance among campylobacter jejuni and campylobacter coli strains isolated from different hosts. | a novel pcr primer pair was used to detect the presence of cmec in 131 campylobacter jejuni and c. coli strains isolated from various hosts (cattle, turkeys, humans, and pigs). dna sequence analysis revealed a high degree of genetic variation between the two species, while extremely limited genetic variation among isolates of the same species was detected. | 2007 | 17652470 |
| development of a real-time fluorescence resonance energy transfer pcr to detect arcobacter species. | a real-time pcr targeting the gyrase a subunit gene outside the quinolone resistance-determining region has been developed to detect arcobacter species. the species identification was done by probe hybridization and melting curve analysis, using fluorescence resonance energy transfer technology. discrimination between arcobacter species was straightforward, as the corresponding melting points showed significant differences with the characteristic melting temperatures of 63.5 degrees c, 58.4 degr ... | 2007 | 17652482 |
| commonality and biosynthesis of the o-methyl phosphoramidate capsule modification in campylobacter jejuni. | in this study we investigated the commonality and biosynthesis of the o-methyl phosphoramidate (meopn) group found on the capsular polysaccharide (cps) of campylobacter jejuni. high resolution magic angle spinning nmr spectroscopy was used as a rapid, high throughput means to examine multiple isolates, analyze the cecal contents of colonized chickens, and screen a library of cps mutants for the presence of meopn. sixty eight percent of c. jejuni strains were found to express the meopn with a hig ... | 2007 | 17675288 |
| prevalence of campylobacter jejuni and campylobacter coli in chicken hybrids with different growth rates, reared according to conventional and "free-range" production methods. | 2007 | 17682919 |