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measles virus replication in lungs of hispid cotton rats after intranasal inoculation.hispid cotton rats were inoculated intranasally with either measles virus (mv) edmonston, a multipassaged, tissue culture-adapted strain of mv, or with one of three clinical mv isolates that had limited passages (three to five times) in tissue culture cells. mv edmonston was recovered from the lungs of every (n = 37) hispid cotton rat inoculated with this virus for at least 7 days after virus inoculation. peak pulmonary titers occurred on day +4 (3.3-4.4 log10/g lung). scattered areas of inflamm ...19921528912
expression of measles virus v protein is associated with pathogenicity and control of viral rna synthesis.nonstructural proteins encoded by measles virus (mv) include the v protein which is translated from an edited p mrna. v protein is not associated with intracellular or released viral particles and has recently been found to be dispensable for mv propagation in cell culture (h. schneider, k. kaelin, and m. a. billeter, virology 227:314-322, 1997). using recombinant mvs (strain edmonston [ed]) genetically engineered to overexpress v protein (ed-v+) or to be deficient for v protein (ed-v-), we foun ...19989733853
cotton rats (sigmodon hispidus): an animal model to study the pathogenesis of measles virus infection.measles is still the most lethal infectious disease of infants worldwide. in spite of research efforts, two major problems associated with measles virus (mv) infection have not been resolved. one is the marked immune suppression leading to subsequent (often lethal) opportunistic infections and the second is waning of maternal antibodies which do not protect against wild type virus infection any longer, but impair vaccination. monkeys are an animal model in which mv infection most closely resembl ...199910065626
replication of clinical measles virus strains in hispid cotton rats.an alternative model to nonhuman primates to study measles virus (mv) pathogenesis, to evaluate potential mv vaccines, or to screen for potential antivirals effective against this virus is highly desirable. the laboratory-adapted edmonston strain of mv has been reported to replicate in the lungs of hispid cotton rats following intranasal inoculation, immunosuppress infected animals, and disseminate widely from the lungs, making these animals a candidate model. however, clinical mv strains have g ...199910320632
dna vaccination with both the haemagglutinin and fusion proteins but not the nucleocapsid protein protects against experimental measles virus infection.plasmids that expressed the nucleocapsid, haemagglutinin and fusion proteins of measles virus (mv) were used to immunize cotton rats (sigmodon hispidus) against intranasal mv infection. after immunization with all three plasmids, t cell responses and mv-specific antibodies were induced. a reduction in virus titre was observed in lung tissue from animals immunized with plasmids expressing the viral glycoproteins. histologically, however, a moderate peribronchitis was observed after immunization w ...200010769075
use of cotton rats to evaluate the efficacy of antivirals in treatment of measles virus infections.no practical animal models for the testing of chemotherapeutic or biologic agents identified in cell culture assays as being active against measles virus (mv) are currently available. cotton rats may serve this purpose. to evaluate this possibility, 5-ethynyl-1-beta-d-ribofuranosylimidazole-4-carboxamide (eicar) and poly(acrylamidomethyl propanesulfonate) (pamps), two compounds that have been reported to inhibit mv in vitro, and ribavirin, an established antiviral drug with mv-inhibitory activit ...200010770743
successful vaccine-induced seroconversion by single-dose immunization in the presence of measles virus-specific maternal antibodies.in humans, maternal antibodies inhibit successful immunization against measles, because they interfere with vaccine-induced seroconversion. we have investigated this problem using the cotton rat model (sigmodon hispidus). as in humans, passively transferred antibodies inhibit the induction of measles virus (mv)-neutralizing antibodies and protection after immunization with mv. in contrast, a recombinant vesicular stomatitis virus (vsv) expressing the mv hemagglutinin (vsv-h) induces high titers ...200010775601
studying experimental measles virus vaccines in the presence of maternal antibodies in the cotton rat model (sigmodon hispidus).the inhibition of vaccine-induced seroconversion after vaccination is one of the problems associated with measles virus (mv) immunization. in cotton rats, after transfer of human mv specific antibodies, vaccine-induced seroconversion is inhibited. with this model, it was shown that plasmid immunization (although successful in seronegative animals) was inhibited by maternal antibodies. in contrast, immunization via a mucosal surface with a vesicular stomatitis virus expressing the mv hemagglutini ...200111257342
successful mucosal immunization of cotton rats in the presence of measles virus-specific antibodies depends on degree of attenuation of vaccine vector and virus dose.after passive transfer of measles virus (mv)-specific antibodies, vaccine-induced seroconversion and subsequent protection is inhibited in cotton rats (sigmodon hispidus). in this system, an attenuated, recombinant vesicular stomatitis virus expressing the mv haemagglutinin (vsv-h) was found previously to induce neutralizing antibodies and protection against mv challenge after intranasal (i.n.) immunization. here it is demonstrated that, after i.n. immunization, vsv-h is found in both lung and b ...200312867646
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