| genetic and serologic analysis of black creek canal virus and its association with human disease and sigmodon hispidus infection. | black creek canal (bcc) virus is a newly identified virus associated with hantavirus pulmonary syndrome (hps) in the southeastern united states. nucleotide sequences were determined for the complete s and m and partial l genomic segments of a bcc virus isolate. phylogenetic analysis indicates that each of the virus segments is unique, and there is no evidence of genetic reassortment having occurred between this and other previously characterized hantaviruses. all hantavirus-seropositive, wild-ca ... | 1995 | 7618284 |
| isolation of black creek canal virus, a new hantavirus from sigmodon hispidus in florida. | numerous rodents were trapped for serologic and virologic studies following the identification of a hantavirus pulmonary syndrome (hps) case in dade county, florida. cotton rats (sigmodon hispidus) were the most frequently capture rodent and displayed the highest seroprevalence to a variety of hantavirus antigens. hantavirus genome rna was detected in all the seropositive cotton rats tested, using a reverse transcriptase-polymerase chain reaction (rt-pcr) assay. a virus was isolated from tissues ... | 1995 | 7623004 |
| hantavirus pulmonary syndrome in florida: association with the newly identified black creek canal virus. | hantavirus pulmonary syndrome (hps) is a recently recognized viral zoonosis. the first recognized cases were caused by a newly described hantavirus. sin nombre virus (previously known as muerto canyon virus), isolated from peromyscus maniculatus (deer mouse). we describe a 33-year-old floridian man who resided outside the ecologic range of p maniculatus but was found to have serologic evidence of a hantavirus infection during evaluation of azotemia associated with adult respiratory distress synd ... | 1996 | 8579086 |
| polarized entry and release in epithelial cells of black creek canal virus, a new world hantavirus. | black creek canal (bcc) virus is a newly identified hantavirus from florida which is carried by the cotton rat (sigmodon hispidus) and is associated with hantavirus pulmonary syndrome (hps). we have investigated the interaction of bcc virus with polarized epithelial cells to examine whether entry and release of this virus occur at specific plasma membrane domains. the polarized vero c1008 monkey kidney cell line was grown on permeable filters and infected with bcc virus either through the apical ... | 1997 | 8995636 |
| genetic reassortment among viruses causing hantavirus pulmonary syndrome. | in order to determine the frequency and characteristics of reassortment among viruses causing hantavirus pulmonary syndrome (hps), mixed infections were initiated in tissue culture by using two closely related strains of sin nombre virus, cc107 (from eastern california) and nmr11 (from new mexico), which share the same species of rodent host in nature, the deer mouse (peromyscus maniculatus). potential reassortant virus plaques were screened by multiplex rt-pcr, using primers specific for indivi ... | 1998 | 9501041 |
| pathogenesis of a north american hantavirus, black creek canal virus, in experimentally infected sigmodon hispidus. | this report describes the first detailed analysis of the replication, persistence, and excretion of a north american hantavirus in its natural rodent reservoir. black creek canal virus was isolated from sigmodon hispidus (cotton rat) shortly after the identification of a hantavirus pulmonary syndrome (hps) case occurring in southern florida. six-week-old male cotton rats were inoculated subcutaneously with 1,000 tissue culture infectious doses. viral complementary rna (vcrna) was quantified as a ... | 1998 | 9684629 |
| black creek canal virus infection in sigmodon hispidus in southern florida. | a total of 1,500 small mammals were collected and tested for antibodies cross-reactive to sin nombre virus (hantavirus: bunyaviridae) at 89 sites in a 1,600 km2 study area of southern florida. more than 95% of the 123 seropositive animals were cotton rats (sigmodon hispidus), suggesting infection by black creek canal virus, although seroreactive rattus rattus (5 of 294) and peromyscus gossypinus (1 of 39) also were captured. crude seroprevalence in s. hispidus was 11%. seroprevalence increased w ... | 1998 | 9840584 |
| transmission of black creek canal virus between cotton rats. | black creek canal (bcc) virus is a hantavirus associated with hantavirus pulmonary syndrome in southeastern north america. the virus was isolated from the spleen of a cotton rat (sigmodon hispidus) trapped in southern florida. our previous studies have shown that we could consistently infect male cotton rats with bcc virus in the laboratory. these animals became persistently infected and virus could be detected in salivary glands, urine, and feces. in this report we show: (1) female and male cot ... | 2000 | 10568766 |
| pathology of black creek canal virus infection in juvenile hispid cotton rats (sigmodon hispidus). | the purpose of this study was to assess the effect of inoculum dose on the pathogenesis of black creek canal virus (bccv) infection in the hispid cotton rat (sigmodon hispidus), the principal host of bccv. no sign of illness was observed in any of the 52 juvenile hispid cotton rats inoculated with 3.1, 1.1, -0.9, or -2.9 log(10) median infectious doses(veroe6) (id(50-veroe6)) of bccv and euthanized on day 9, 18, 27, or 54 postinoculation (pi). analysis of virus assay and serological data indicat ... | 2010 | 20455779 |