detection of turkey enteric coronavirus by enzyme-linked immunosorbent assay and differentiation from other coronaviruses.a double-antibody elisa for the detection of coronaviruses in intestinal contents from turkey poults with diarrhea was developed. antibodies were raised in rabbits and guinea pigs against a minnesota isolate of turkey enteric coronavirus (tcv) propagated in embryonating turkey eggs and were purified by density-gradient centrifugation. the specificity of antisera was confirmed by hemagglutination-inhibition and immunoelectron microscopy. absorption of anti-tcv hyperimmune sera with egg extracts o ...19892541641
physicochemical properties of transmissible gastroenteritis virus hemagglutinin.transmissible gastroenteritis virus was readily adsorbed onto chicken erythrocytes at 4 degrees c. the hemagglutinin thus adsorbed could be eluted from the erythrocytes by incubating in phosphate buffered saline at 37 degrees c. the receptor on chicken erythrocytes for the hemagglutinin was inactivated by neuraminidase and potassium periodate, but not by trypsin, 2-mercaptoethanol and formalin. the hemagglutinin was inactivated by trypsin, papain, pepsin, alpha-amylase, phospholipase c, neuramin ...19882835945
cloning, sequencing and expression of the s protein gene from two geographically distinct strains of canine coronavirus.the gene encoding the spike (s) protein from two geographically distinct strains (american and british) of canine coronavirus (ccv) was cloned and sequenced. the nucleotide sequence revealed open reading frames of 1443 or 1453 amino acids, respectively. structural features include an n-terminal hydrophobic signal sequence, a hydrophilic cysteine-rich cluster near the c-terminus, two heptad repeats and 29 or 33 potential n-glycosylation sites. pairwise comparisons of s amino acid sequences from t ...19958607285
establishment of a stable cho cell line with high level expression of recombinant porcine ifn-ß.a cho cell clone (cho-poifn-ß) with stable porcine ifn-ß expression under control of cmv promoter was selected under g418 pressure. in a 25cm(2) cell culture flask (5 ml culture medium), the cumulative protein yield of recombinant poifn-ß reached 2.3×10(6) iu/ml. this cells clone maintained stable expression for at least 20 generations even in the absence of g418 selection pressure. the expressed recombinant poifn-ß could induce the expression of porcine mx protein in pk15 cells, and activate th ...201121459017
preparation and physicochemical property of chicken yolk immunoglobulin (igy) against porcine transmissible gastroenteritis virus (tgev).oral administration of immunoglobulin prepared from the egg yolk of hens immunized with porcine transmissible gastroenteritis virus (tgev) has been demonstrated to reduce piglets mortality significantly in our previous studies. in the present study, we investigated the stability of chicken yolk immunoglobulin (igy) specific to tgev by measuring the remaining activity by elisa. the results showed that the igy was stable between ph 4 and ph 11. in the incubation with pepsin at ph 4 and ph 6, about ...200932214987
coronaviruses from pheasants (phasianus colchicus) are genetically closely related to coronaviruses of domestic fowl (infectious bronchitis virus) and turkeys.reverse-transcriptase polymerase chain reactions (rt-pcrs) were used to examine rna extracted from mouth/nasal swabs from pheasants exhibiting signs of respiratory disease. the oligonucleotides used were based on sequences of infectious bronchitis virus (ibv), the coronavirus of domestic fowl. a rt-pcr for the highly conserved region ii of the 3' untranslated region of the ibv genome detected a coronavirus in swabs from 18/21 estates. sequence identity with the corresponding region of ibvs and c ...200212425795
hemagglutination with transmissible gastroenteritis virus.transmissible gastroenteritis virus grown in primary swine kidney cell cultures agglutinated erythrocytes from chicken, guinea pig and cattle but not erythrocytes from mouse and goose. the optimal incubation temperature was at 4 degrees c. the hemagglutination (ha) reaction was inhibited by specific antiserum. some factors involved in the ha and ha-inhibition (hi) were investigated and standard ha and hi tests were established. hi antibody titers of individual pig sera showed a significant posit ...19873039944
chicken egg yolk antibodies (igy) as non-antibiotic production enhancers for use in swine production: a recent years, the use of in-feed antibiotics for growth and disease prevention in livestock production has been under severe scrutiny. the use and misuse of in-feed antibiotics has led to problems with drug residues in animal products and increased bacterial resistance. chicken egg yolk antibodies (igy) have attracted considerable attention as an alternative to antibiotics to maintain swine health and performance. oral administration of igy possesses many advantages over mammalian igg such as ...201526309735
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