newcastle disease virus (ndv) marker vaccine: an immunodominant epitope on the nucleoprotein gene of ndv can be deleted or replaced by a foreign epitope.the nucleoprotein (np) of newcastle disease virus (ndv) functions primarily to encapsidate the virus genome for the purpose of rna transcription, replication, and packaging. this conserved multifunctional protein is also efficient in inducing ndv-specific antibody in chickens. here, we localized a conserved b-cell immunodominant epitope (ide) spanning residues 447 to 455 and successfully generated a recombinant ndv lacking the ide by reverse genetics. despite deletion of np residues 443 to 460 e ...200212239288
coronaviruses from pheasants (phasianus colchicus) are genetically closely related to coronaviruses of domestic fowl (infectious bronchitis virus) and turkeys.reverse-transcriptase polymerase chain reactions (rt-pcrs) were used to examine rna extracted from mouth/nasal swabs from pheasants exhibiting signs of respiratory disease. the oligonucleotides used were based on sequences of infectious bronchitis virus (ibv), the coronavirus of domestic fowl. a rt-pcr for the highly conserved region ii of the 3' untranslated region of the ibv genome detected a coronavirus in swabs from 18/21 estates. sequence identity with the corresponding region of ibvs and c ...200212425795
coronavirus pathogenesis and the emerging pathogen severe acute respiratory syndrome coronavirus.coronaviruses are a family of enveloped, single-stranded, positive-strand rna viruses classified within the nidovirales order. this coronavirus family consists of pathogens of many animal species and of humans, including the recently isolated severe acute respiratory syndrome coronavirus (sars-cov). this review is divided into two main parts; the first concerns the animal coronaviruses and their pathogenesis, with an emphasis on the functions of individual viral genes, and the second discusses t ...200516339739
h5n1 influenza marker vaccine for serological differentiation between vaccinated and infected chickens.using plasmid-based reverse genetics, we generated a molecularly altered virus, h5n1/pr8-5b19, containing modified ha and na genes from a/goose/guangdong/1/96 (gs/gd/1/96). in the h5n1/pr8-5b19 virus, the ha cleavage site was modified to resemble that of low-pathogenic avian strains and a portion of the na stalk region was replaced by the immunodominant 5b19 epitope of the s2 glycoprotein of murine hepatitis virus (mhv). h5n1/pr8-5b19 is not lethal to embryonated eggs or chickens. chickens immun ...200818501701
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