Publications

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detection of turkey enteric coronavirus by enzyme-linked immunosorbent assay and differentiation from other coronaviruses.a double-antibody elisa for the detection of coronaviruses in intestinal contents from turkey poults with diarrhea was developed. antibodies were raised in rabbits and guinea pigs against a minnesota isolate of turkey enteric coronavirus (tcv) propagated in embryonating turkey eggs and were purified by density-gradient centrifugation. the specificity of antisera was confirmed by hemagglutination-inhibition and immunoelectron microscopy. absorption of anti-tcv hyperimmune sera with egg extracts o ...19892541641
complement-dependent neutralization of transmissible gastroenteritis virus by monoclonal antibodies.monoclonal antibodies (mab) to each of the 3 major structural proteins of transmissible gastroenteritis virus (tgev) of swine were compared as to their virus neutralizing activity in the presence or absence of guinea pig complement. mabs to the peplomer protein had neutralizing activity for tgev with or without complement and the titers were similar in either case. mabs to the matrix protein had neutralizing activity for tgev only in the presence of complement. antibodies to the nucleocapsid pro ...19872829565
[isolation of a concentrated purified preparation of the transmissible gastroenteritis virus of swine and a study of its antigenic properties].pretreatment of the transmissible gastroenteritis virus of swine (tge) (coronavirus) with trypsin increased the yield of the infectious virus approximately 100-fold when propagated in roller cultures of spev cells, the time required for the development of marked cpe being twice shorter than without treatment. the method of concentration and purification of tge virus is described which yields a preparative amount of highly purified virions retaining their structure. such preparations were used fo ...19872833035
neutralization of porcine transmissible gastroenteritis virus by complement-dependent monoclonal antibodies.monoclonal antibodies (mab) to each of the 3 major structural proteins of transmissible gastroenteritis virus (tgev) of swine were compared, using their virus-neutralizing (vn) activity in the presence or absence of guinea pig, rabbit, or swine complement. the mab against the peplomer protein had similar vn titers for tgev in the presence or absence of complement from any source. the mab against the matrix protein had vn activity for tgev only in the presence of complement, whereas mab specific ...19882833862
[development of test systems for immunoenzyme analysis for the detection of the antigen of the transmissible gastroenteritis virus of swine].direct and indirect variants of enzyme immunoassay (eia) were developed for the detection of specific antigen of swine transmissible gastroenteritis virus (tgev). preparation of igg were obtained from highly specific hyperimmune sera of guinea pigs and gnotobiotic piglets and their optimal concentration for the tests were determined. for the direct eia, a peroxidase conjugate was prepared from guinea pig igg and its working dilution was determined. the developed eia systems proved to be highly s ...19882845669
hemagglutination with transmissible gastroenteritis virus.transmissible gastroenteritis virus grown in primary swine kidney cell cultures agglutinated erythrocytes from chicken, guinea pig and cattle but not erythrocytes from mouse and goose. the optimal incubation temperature was at 4 degrees c. the hemagglutination (ha) reaction was inhibited by specific antiserum. some factors involved in the ha and ha-inhibition (hi) were investigated and standard ha and hi tests were established. hi antibody titers of individual pig sera showed a significant posit ...19873039944
direct immunoelectron microscopy of transmissible gastroenteritis virus with immunoglobulins g and a and guinea pig complement.porcine colostral immunoglobulin (ig)g and iga, isolated from transmissible gastroenteritis virus-infected sows, were compared by direct immunoelectron microscopy. it was estimated, using antibodies with a less than a twofold difference in virus-neutralizing activity, that igg was 500 times more efficient than was iga for coating transmissible gastroenteritis virions. guinea pig complement enhanced the antibody coating with igg, but did not increase virus-neutralizing activity of igg or iga.19806244762
prospects for the use of spherical gold nanoparticles in immunization.recent years have seen extremely fast development of new viral nanovaccines and diagnostic agents using nanostructures prepared by biological and chemical synthesis. we used spherical gold nanoparticles (average diameter, 15 nm) as a platform for the antigen for swine transmissible gastroenteritis virus (tgev). the literature data demonstrate that immunization of animals with the tgev antigen coupled to gold nanoparticles (gnps) not only activates antigen-presenting cells but also increases the ...201930402771
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