| calves are susceptible to infection with the newly emerged porcine deltacoronavirus, but not with the swine enteric alphacoronavirus, porcine epidemic diarrhea virus. | fecal virus shedding, seroconversion and histopathology were evaluated in 3-7-year-old gnotobiotic calves orally inoculated with porcine deltacoronavirus (pdcov) (9.0-9.6 log10 genomic equivalents [ge] of oh-fd22-p5; n=4) or porcine epidemic diarrhea virus (pedv) (10.2-12.5 log10 ge of pc21a; n=3). in pdcov-inoculated calves, an acute but persisting fecal viral rna shedding and pdcov-specific serum igg antibody responses were observed, but without lesions or clinical disease. however, no fecal s ... | 2017 | 28374120 |
| characterization of a novel bat-hku2-like swine enteric alphacoronavirus (seacov) infection in cultured cells and development of a seacov infectious clone. | swine enteric alphacoronavirus (seacov), also known as swine acute diarrhea syndrome coronavirus (sads-cov), belongs to the species rhinolophus bat coronavirus hku2. herein, we report on the primary characterization of seacov in vitro. four antibodies against the seacov spike, membrane, nucleocapsid and nonstructural protein 3 capable of reacting with viral antigens in seacov-infected vero cells were generated. we established a dna-launched seacov infectious clone based on the cell adapted passa ... | 2019 | 31419711 |
| porcine epidemic diarrhea virus deficient in rna cap guanine-n-7 methylation is attenuated and induces higher type i and iii interferon responses. | the 5' cap methylation of viral rna plays important roles in rna stability, efficient translation, and immune evasion. thus, rna cap methylation is an attractive target for antiviral discovery and development of new live attenuated vaccines. for coronaviruses, rna cap structure is first methylated at the guanine-n-7 (g-n-7) position by nonstructural protein 14 (nsp14), which facilitates and precedes the subsequent ribose 2'-o methylation by the nsp16-nsp10 complex. using porcine epidemic diarrhe ... | 2020 | 32461321 |
| broad cross-species infection of cultured cells by bat hku2-related swine acute diarrhea syndrome coronavirus and identification of its replication in murine dendritic cells in vivo highlight its potential for diverse interspecies transmission. | outbreaks of severe diarrhea in neonatal piglets in guangdong, china, in 2017 resulted in the isolation and discovery of a novel swine enteric alphacoronavirus (seacov) derived from the species rhinolophus bat coronavirus hku2 (y. pan, x. tian, p. qin, b. wang, et al., vet microbiol 211:15-21, 2017). seacov was later referred to as swine acute diarrhea syndrome cov (sads-cov) by another group (p. zhou, h. fan, t. lan, x.-l. yang, et al., nature 556:255-258, 2018). the present study was set up to ... | 2019 | 31554686 |
| discovery of a novel swine enteric alphacoronavirus (seacov) in southern china. | outbreaks of diarrhea in newborn piglets without detection of transmissible gastroenteritis virus (tgev), porcine epidemic diarrhea virus (pedv) and porcine deltacoronavirus (pdcov), have been recorded in a pig farm in southern china since february 2017. isolation and propagation of the pathogen in cell culture resulted in discovery of a novel swine enteric alphacoronavirus (tentatively named seacov) related to the bat coronavirus hku2 identified in the same region a decade ago. specific fluores ... | 2017 | 29102111 |
| swine enteric alphacoronavirus (swine acute diarrhea syndrome coronavirus): an update three years after its discovery. | discovered in 2017, swine enteric alphacoronavirus (seacov), also known as swine acute diarrhea syndrome coronavirus (sads-cov) or porcine enteric alphacoronavirus (peav), is the fifth porcine cov identified in diarrheal piglets. the presumed name "sads-cov" may not be appropriate since current studies have not provided strong evidence for high pathogenicity of the virus. seacov was the most recently recognized cov of potential bat origin prior to the novel human severe acute respiratory syndrom ... | 2020 | 32482591 |