production of polyclonal antibodies against pelargonium zonate spot virus coat protein expressed in escherichia coli and application for immunodiagnosis.pelargonium zonate spot virus (pzsv) is identified recently in tomato plants in the united states. to develop serological diagnostic tools for the detection of this virus, the production of good quality antibodies is a necessity. the coat protein (cp) gene of a california isolate of pzsv was cloned into a bacterial expression vector (ptriex-4 ek/lic). the plasmid ptriex-4-pzsv-cp was transformed into escherichia coli rosetta 2(de3)placi and the recombinant pzsv-cp was expressed as a fusion prote ...200919397928
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