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a quantitative pcr method for assessing the presence of pasteurella testudinis dna in nasal lavage samples from the desert tortoise (gopherus agassizii).pasteurella testudinis has been associated with upper respiratory tract disease (urtd) in the threatened desert tortoise (gopherus agassizii). our goal was to develop a sensitive and specific qpcr method for detecting dna from p. testudinis in nasal lavage fluid collected from desert tortoises in the field. probes for 16s ribosomal rna and rna polymerase β-subunit (rpob) genes were designed. a standard curve generated with dna extracted from known numbers of bacterial cells determined by flow cy ...201223000631
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