Publications

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design and validation of four new primers for next-generation sequencing to target the 18s rrna genes of gastrointestinal ciliate protozoa.four new primers and one published primer were used to pcr amplify hypervariable regions within the protozoal 18s rrna gene to determine which primer pair provided the best identification and statistical analysis. pcr amplicons of 394 to 498 bases were generated from three primer sets, sequenced using roche 454 pyrosequencing with titanium, and analyzed using the blast database (ncbi) and mothur version 1.29. the protozoal diversity of rumen contents from moose in alaska was assessed. in the pre ...201424973070
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