| isolation of a novel insect-specific flavivirus from culiseta melanura in the northeastern united states. | the genus flavivirus includes a number of newly recognized viruses that infect and replicate only within mosquitoes. to determine whether insect-specific flaviviruses (isfs) may infect culiseta (cs.) melanura mosquitoes, we screened pools of field-collected mosquitoes for virus infection by rt-pcr targeting conserved regions of the ns5 gene. ns5 nucleotide sequences amplified from cs. melanura pools were genetically similar to other isfs and most closely matched calbertado virus from culex tarsa ... | 2016 | 26807512 |