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purification and coat protein gene sequence of a montana rmv-like isolate of barley yellow dwarf virus.a montana barley yellow dwarf virus (bydv) isolate, bydv-rmv-mt, is serologically identical to the new york rmv type isolate (rmv-ny) but differs in aphid transmission phenotype. a purification procedure for bydv-rmv-mt was developed and cdnas encompassing the entire coat protein gene and a portion of the putative polymerase gene of both rmv-mt and rmv-ny were cloned and sequenced. diameters of rmv-mt virions averaged 24.7 nm. average virus yield was 4.2 mg/kg plant tissue. there was 81% sequenc ...19968645094
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