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detection of c. botulinum types in honey by mpcr.the aim of this study was to determine the prevalence of clostridium botulinum in honey samples using conventional methods and multiplex pcr (mpcr). a total number of 150 honey samples were randomly collected from apiaries, retail shops, weekly open bazaars, and supermarkets in samsun, turkey. of 150 honey samples, 4 (2.6%) were positive for the botulinum neurotoxin gene by mpcr analysis. a total of 4 c. botulinum isolates were obtained from the mpcr positive samples, of which 3 were type a and ...201424621137
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