| distribution of naturally occurring norovirus genogroups i, ii, and iv in oyster tissues. | this study evaluated different tissues of naturally contaminated oysters (crassostrea belcheri) for the presence of noroviruses. rna from digestive tissues, gills, and mantle of the oysters was extracted and tested for norovirus genogroup (g) i, gii, and giv using rt-nested pcr. in spiking experiments with a known norovirus, gii.4, the detection limits were 2.97 × 10(2) rna copies/g of digestive tissues, 2.62 × 10(2) rna copies/g of gills, and 1.61 × 10(3) rna copies/g of mantle. a total of 85 o ... | 2017 | 28550646 |
| prevalence and molecular genotyping of noroviruses in market oysters, mussels, and cockles in bangkok, thailand. | noroviruses are the most common cause of acute gastroenteritis associated with bivalve shellfish consumption. this study aimed to detect and characterize noroviruses in three bivalve shellfish species: oysters (saccostrea forskali), cockles (anadara nodifera), and mussels (perna viridis). the virus concentration procedure (adsorption-twice elution-extraction) and a molecular method were employed to identify noroviruses in shellfish. rt-nested pcr was able to detect known norovirus gii.4 of 8.8 × ... | 2016 | 26872638 |