detection of hepatozoon felis in ticks collected from free-ranging amur tigers ( panthera tigris altaica), russian far east, 2002-12.we collected 69 ticks from nine, free-ranging amur tigers ( panthera tigris altaica) between 2002 and 2011 and investigated them for tick-borne pathogens. dna was extracted using alkaline digestion and pcr was performed to detect apicomplexan organisms. partial 18s rdna amplification products were obtained from 14 ticks from four tigers, of which 13 yielded unambiguous nucleotide sequence data. comparative sequence analysis revealed all 13 partial 18s rdna sequences were most similar to those be ...201627243154
Displaying items 1 - 1 of 1